| 1. |
- Kobel, M., et al.
(författare)
-
p53 and ovarian carcinoma survival: an Ovarian Tumor Tissue Analysis consortium study
- 2023
-
Ingår i: Journal of Pathology Clinical Research. - : Wiley. - 2056-4538. ; 9:3, s. 208-222
-
Tidskriftsartikel (refereegranskat)abstract
- Our objective was to test whether p53 expression status is associated with survival for women diagnosed with the most common ovarian carcinoma histotypes (high-grade serous carcinoma [HGSC], endometrioid carcinoma [EC], and clear cell carcinoma [CCC]) using a large multi-institutional cohort from the Ovarian Tumor Tissue Analysis (OTTA) consortium. p53 expression was assessed on 6,678 cases represented on tissue microarrays from 25 participating OTTA study sites using a previously validated immunohistochemical (IHC) assay as a surrogate for the presence and functional effect of TP53 mutations. Three abnormal expression patterns (overexpression, complete absence, and cytoplasmic) and the normal (wild type) pattern were recorded. Survival analyses were performed by histotype. The frequency of abnormal p53 expression was 93.4% (4,630/4,957) in HGSC compared to 11.9% (116/973) in EC and 11.5% (86/748) in CCC. In HGSC, there were no differences in overall survival across the abnormal p53 expression patterns. However, in EC and CCC, abnormal p53 expression was associated with an increased risk of death for women diagnosed with EC in multivariate analysis compared to normal p53 as the reference (hazard ratio [HR] = 2.18, 95% confidence interval [CI] 1.36-3.47, p = 0.0011) and with CCC (HR = 1.57, 95% CI 1.11-2.22, p = 0.012). Abnormal p53 was also associated with shorter overall survival in The International Federation of Gynecology and Obstetrics stage I/II EC and CCC. Our study provides further evidence that functional groups of TP53 mutations assessed by abnormal surrogate p53 IHC patterns are not associated with survival in HGSC. In contrast, we validate that abnormal p53 IHC is a strong independent prognostic marker for EC and demonstrate for the first time an independent prognostic association of abnormal p53 IHC with overall survival in patients with CCC.
|
|
| 2. |
- Langer, Judith, et al.
(författare)
-
Present and Future of Surface-Enhanced Raman Scattering
- 2020
-
Ingår i: ACS Nano. - : American Chemical Society (ACS). - 1936-086X .- 1936-0851. ; 14:1, s. 28-117
-
Forskningsöversikt (refereegranskat)abstract
- The discovery of the enhancement of Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and analytical techniques. Significant experimental and theoretical effort has been directed toward understanding the surface-enhanced Raman scattering (SERS) effect and demonstrating its potential in various types of ultrasensitive sensing applications in a wide variety of fields. In the 45 years since its discovery, SERS has blossomed into a rich area of research and technology, but additional efforts are still needed before it can be routinely used analytically and in commercial products. In this Review, prominent authors from around the world joined together to summarize the state of the art in understanding and using SERS and to predict what can be expected in the near future in terms of research, applications, and technological development. This Review is dedicated to SERS pioneer and our coauthor, the late Prof. Richard Van Duyne, whom we lost during the preparation of this article. ©
|
|
| 3. |
- Hakonen, Aron, 1970, et al.
(författare)
-
Hand-Held Femtogram Detection of Hazardous Picric Acid with Hydrophobic Ag Nanopillar SERS Substrates and Mechanism of Elasto-Capillarity
- 2017
-
Ingår i: ACS Sensors. - : American Chemical Society (ACS). - 2379-3694. ; 2:2, s. 198-202
-
Tidskriftsartikel (refereegranskat)abstract
- Picric acid (PA) is a severe environmental and security risk due to its unstab e, toxic, and explosive properties. It is also challenging to detect in trace amounts and in situ because of its highly acidic and anionic character. Here, we assess sensing of PA under nonlaboratory conditions using surface-enhanced Raman scattering (SERS) silver nanopillar substrates and handheld Raman spectroscopy equipment. The advancing elasto-capillarity effects are explained by molecular dynamics simulations. We obtain a SERS PA detection limit on the order of 20 ppt, corresponding attomole amounts, which together with the simple analysis methodology demonstrates that the presented approach is highly competitive for ultrasensitive analysis in the field.
|
|
| 4. |
- Tenje, Maria, et al.
(författare)
-
Cantilver based sensors
- 2013
-
Ingår i: Optochemical Nanosensors. - : CRC Press. - 9781439854891 - 9780429063169
-
Bokkapitel (refereegranskat)
|
|
| 5. |
|
|
| 6. |
- Andersson, Malte, 1941, et al.
(författare)
-
”Minskande befolkning är inte problemet”
- 2020
-
Ingår i: Dagens Nyheter. ; :1 augusti, DN-debatt
-
Tidskriftsartikel (populärvet., debatt m.m.)abstract
- Nätverket Population Matters Sweden: En uppmärksammad studie i The Lancet pekar mot en lägre befolkningsökning i världen än tidigare prognoser. Men en miljard människor till är fortfarande långt över vad jorden klarar. Befolkningstrenden måste snarare vända neråt, och det kräver åtgärder för att stärka kvinnors rättigheter världen över.
|
|
| 7. |
- BENBADIS, L, et al.
(författare)
-
WORKING GROUP-VII - FOOD-PRODUCTS
- 1995
-
Ingår i: MICROBIAL ECOLOGY IN HEALTH AND DISEASE. - 0891-060X. ; 8, s. S43-S44
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
|
|
| 8. |
- Forsen, E, et al.
(författare)
-
Dry release of suspended nanostructures
- 2004
-
Ingår i: Microelectronic Engineering. - 1873-5568. ; 73-74, s. 487-490
-
Tidskriftsartikel (refereegranskat)abstract
- A dry release method for fabrication of suspended nanostructures is presented. The technique has been combined with an anti-stiction treatment for fabrication of nanocantilever based nanoelectromechanical systems (NEMS). The process combines a dry release method, using a supporting layer of photoresist which is removed using oxygen ashing in a reactive ion etcher (RIE), with CHF3 plasma induced deposition of an fluorocarbon (FC) film acting as an antistiction coating. All in a single RIE sequence. The dry release process is contamination free and batch process compatible. Furthermore, the technique enables long time storage and transportation of produced devices without the risk of stiction. By combining the dry release method with a plasma deposited anti-stiction coating both fabrication induced stiction, which is mainly caused by capillary forces originating from the dehydration of meniscuses formed between suspended structures and the substrate during processing, as well as in-use stiction, occurring during mechanical operation of the system, are avoided. (C) 2004 Elsevier B.V. All rights reserved.
|
|
| 9. |
|
|
| 10. |
- Forsen, E, et al.
(författare)
-
Fabrication of cantilever based mass sensors integrated with CMOS using direct write laser lithography on resist
- 2004
-
Ingår i: Nanotechnology. - : IOP Publishing. - 0957-4484 .- 1361-6528. ; 15:10, s. 628-633
-
Tidskriftsartikel (refereegranskat)abstract
- A CMOS compatible direct write laser lithography technique has been developed for cantilever fabrication on pre-fabricated standard CMOS. We have developed cantilever based sensors for mass measurements in vacuum and air. The cantilever is actuated into lateral vibration by electrostatic excitation and the resonant frequency is detected by capacitive readout. The device is integrated on standard CMOS circuitry. In the work a new direct write laser lithography (DWL) technique is introduced. This laser lithography technique is based on direct laser writing on substrates coated with a resist bi-layer consisting of poly(methyl methacrylate) (PMMA) on lift-off resist (LOR). Laser writing evaporates the PMMA, exposing the LOR. A resist solvent is used to transfer the pattern down to the substrate. Metal lift-off followed by reactive ion etching is used for patterning the structural poly-Si layer in the CMOS. The developed laser lithography technique is compatible with resist exposure techniques such as electron beam lithography. We demonstrate the fabrication of sub-micrometre wide suspended cantilevers as well as metal lift-off with feature line widths down to approximately 500 nm.
|
|
| 11. |
|
|
| 12. |
- Forsen, E, et al.
(författare)
-
Laser lithography on resist bi-layer for nanoelectromechanical systems prototyping
- 2004
-
Ingår i: Microelectronic Engineering. - 1873-5568. ; 73-74, s. 491-495
-
Tidskriftsartikel (refereegranskat)abstract
- We present a laser lithography technique based on lift-off, for fast and flexible prototyping of micro and nanoelectromechanical systems (MEMS/NEMS). The technique is based on direct laser writing on substrates coated with a resist bi-layer consisting of polymethyl methacrylate (PMMA) on lift-off resist (LOR). Laser writing melts and evaporates the PMMA exposing the LOR. Oxygen ashing removes PMMA residues within the lithography pattern. A resist solvent is used to transfer the pattern down to the substrate. The LOR is dissolved isotropically while the PMMA is unaffected by the solvent, hence creating an undercut profile. After metal evaporation a two-step lift-off process prevents metal flakes from adhering to the surface. First, warm acetone dissolves the PMMA and lifts off the metal layer, then warm Remover PG removes the LOR and any remaining metal. Metal structures with line widths down to 600 nm and dots with 600 mn diameters are presented. (C) 2004 Elsevier B.V. All rights reserved.
|
|
| 13. |
- Forsen, E, et al.
(författare)
-
Ultrasensitive mass sensor fully integrated with complementary metal-oxide-semiconductor circuitry
- 2005
-
Ingår i: Applied Physics Letters. - : AIP Publishing. - 0003-6951 .- 1077-3118. ; 87:4
-
Tidskriftsartikel (refereegranskat)abstract
- Nanomechanical resonators have been monolithically integrated on preprocessed complementary metal-oxide-semiconductor (CMOS) chips. Fabricated resonator systems have been designed to have resonance frequencies up to 1.5 MHz. The systems have been characterized in ambient air and vacuum conditions and display ultrasensitive mass detection in air. A mass sensitivity of 4 ag/Hz has been determined in air by placing a single glycerine drop, having a measured weight of 57 fg, at the apex of a cantilever and subsequently measuring a frequency shift of 14.8 kHz. CMOS integration enables electrostatic excitation, capacitive detection, and amplification of the resonance signal directly on the chip.
|
|
| 14. |
- Ghatnekar-Nilsson, Sara, et al.
(författare)
-
Resonators with integrated CMOS circuitry for mass sensing applications, fabricated by electron beam lithography
- 2005
-
Ingår i: Nanotechnology. - : IOP Publishing. - 0957-4484 .- 1361-6528. ; 16:1, s. 98-102
-
Tidskriftsartikel (refereegranskat)abstract
- A resonator system has been fabricated directly on a pre-processed CMOS chip. The system is to be used for high sensitivity mass sensing applications in air and vacuum. The resonator system, corresponding of a cantilever and structures for electrostatic actuation and capacitive read-out, have been defined by electron beam lithography on top of a charge and radiation sensitive CMOS layer in predefined areas as a post-process step. This has been accomplished without affecting the electronic properties of the pre-processed CMOS circuits. The subsequent etching steps to fully release the cantilevers have been obtained without stiction of the cantilevers to the substrate. Cantilevers are driven at their mechanical resonance in a lateral mode, and the frequency is monitored by capacitive read-out on the chip. CMOS integration enables signal detection directly on the chip, which radically decreases the parasitic capacitances. Consequently, low-noise electrical measurements with a very high mass sensitivity are obtained. Fabricated resonator systems were characterized to have resonance frequencies of approximately 1.49 MHz, which is in good agreement with a theoretical estimation of 1.41 MHz. The theoretical mass resolution, partial derivativem/partial derivativef, is approximately 17 ag Hz(-1) using a Young modulus value of 160 GPa.
|
|
| 15. |
- Hakonen, Aron, 1970, et al.
(författare)
-
Detection of nerve gases using surface-enhanced Raman scattering substrates with high droplet adhesion
- 2016
-
Ingår i: Nanoscale. - : Royal Society of Chemistry (RSC). - 2040-3372 .- 2040-3364. ; 8:3, s. 1305-1308
-
Tidskriftsartikel (refereegranskat)abstract
- Threats from chemical warfare agents, commonly known as nerve gases, constitute a serious security issue of increasing global concern because of surging terrorist activity worldwide. However, nerve gases are difficult to detect using current analytical tools and outside dedicated laboratories. Here we demonstrate that surface-enhanced Raman scattering (SERS) can be used for sensitive detection of femtomol quantities of two nerve gases, VX and Tabun, using a handheld Raman device and SERS substrates consisting of flexible gold-covered Si nanopillars. The substrate surface exhibits high droplet adhesion and nanopillar clustering due to elasto-capillary forces, resulting in enrichment of target molecules in plasmonic hot-spots with high Raman enhancement. The results may pave the way for strategic life-saving SERS detection of chemical warfare agents in the field.
|
|
| 16. |
- Juhlin, Lars, et al.
(författare)
-
Selective surface-enhanced Raman scattering detection of Tabun, VX and Cyclosarin nerve agents using 4-pyridine amide oxime functionalized gold nanopillars
- 2020
-
Ingår i: Talanta. - : Elsevier BV. - 0039-9140 .- 1873-3573. ; 211
-
Tidskriftsartikel (refereegranskat)abstract
- We have earlier demonstrated sensitive detection of low the volatile nerve agents Tabun, Cyclosarin and VX by using handheld Raman instrumentation in conjunction with surface-enhanced Raman scattering (SERS) attained with gold and silver coated Si nanopillar substrates. In the present proof-of-concept study, the gold substrates chemically are functionalized to realize selectivity towards organophosphorus compounds (OPs) with high sensitivity. A potential capturer and reporter molecule, chemical nerve agent antidote, 4-pyridine amide oxime, is evaluated due to its high Raman cross section, high chemical affinity towards gold, and binding specificity to the target substances Tabun, VX and Cyclosarin via the oxime group. Upon selective and covalent binding, the SERS probe undergoes structural changes which are reflected in the spectral SERS responses, making it suitable for indirect monitoring of nerve agents in aqueous solution. With the probe attached to the hotspots of Au-coated Si nanopillars, the SERS signals distinctly discriminate between specific and non-specific analyte binding of Tabun, Cyclosarin and VX down to sub ppm levels. SERS spectrum of 4-PAO is measured after microliter drop coating of aqueous sample solution onto the functionalized substrates and subsequent water evaporation from surfaces. This binding assay is complemented by letting functionalized substrates being immersed into sample solutions 1 h before measuring. Binding specific SERS response decreases in following order: Tabun > VX > Cyclosarin. Overall, the concept looks promising, as expected the candidate probe 4-PAO introduces selectivity to the nanopillar gold substrates without loss of sensitivity.
|
|
| 17. |
- Li, Haipeng, et al.
(författare)
-
Democratizing robust SERS nano-sensors for food safety diagnostics
- 2023
-
Ingår i: Chemical Engineering Journal. - 1385-8947 .- 1873-3212. ; 470
-
Tidskriftsartikel (refereegranskat)abstract
- Pesticide residues in food products cause human health concerns through food contamination, thereby necessitating their rapid and facile detection. Although surface-enhanced Raman scattering (SERS) technique can rapidly and reliably detect pesticide residues, its application in food safety diagnostics is restricted by its high expense, low scalability, and low reproducibility of the necessary sensors. Herein, we present a low-cost, large-scale, and highly reproducible nanofabrication route for SERS nano-sensors, based on the thermophoresis-assisted direct deposition of plasmonic core–shell structured Ag-SiO2 nanoparticles produced in the gas phase, on temperature-controlled inexpensive glass substrates. The high-performance SERS substrates were fabricated at a laboratory production rate of 100 samples/hour, demonstrating the scalability and cost-effectiveness of our aerosol manufacturing strategy. Our highly sensitive SERS substrates rapidly and quantitatively detected pesticide residues in fresh orange, indicating their practical applicability for food safety diagnostics.
|
|
| 18. |
- Nissen, NI, et al.
(författare)
-
Prognostic value of blood-based fibrosis biomarkers in patients with metastatic colorectal cancer receiving chemotherapy and bevacizumab
- 2021
-
Ingår i: Scientific reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 11:1, s. 865-
-
Tidskriftsartikel (refereegranskat)abstract
- A desmoplastic colorectal cancer stroma, characterized by excess turnover of the cancer-associated fibroblast derived collagens type III and VI, can lead to reduced drug-uptake and poor treatment response. We investigated the association between biomarkers of collagen type III and VI and overall survival (OS) in patients with metastatic colorectal cancer (mCRC). Serum samples were collected from 252 patients with mCRC prior to treatment with bevacizumab and chemotherapy. Serum concentrations of biomarkers reflecting formation of collagen type III (PRO-C3) and VI (PRO-C6) and degradation of collagen type VI (C6M and C6Mα3) were determined by ELISA. The biomarkers were evaluated for associations with OS, individually, combined, and after adjusting for carcinoembryonic antigen (CEA), lactate dehydrogenase (LDH) and performance status (PS). High baseline levels (> median) of each collagen biomarker were significantly associated with shorter OS (PRO-C3: HR = 2.0, 95%CI = 1.54–2.63; PRO-C6: HR = 1.6, 95%CI = 1.24–2.11; C6M: HR = 1.4, 95%CI = 1.05–1.78; C6Mα3: HR = 1.6, 95%CI = 1.16–2.07). PRO-C3 and PRO-C6 remained significant after adjustment for CEA, LDH and PS. Weak correlations were seen between the collagen biomarkers (r = 0.03–0.59) and combining all improved prognostic capacity (HR = 3.6, 95%CI = 2.30–5.76). Collagen biomarkers were predictive of shorter OS in patients with mCRC. This supports that collagen- and CAF biology is important in CRC.
|
|
| 19. |
- Tenje, Maria, et al.
(författare)
-
Cantilever-based sensors
- 2016
-
Ingår i: Optochemical Nanosensors. - 9781439854907 - 9781439854891 ; , s. 119-145
-
Bokkapitel (refereegranskat)
|
|
| 20. |
- Wu, K., et al.
(författare)
-
Wafer-Scale Leaning Silver Nanopillars for Molecular Detection at Ultra-Low Concentrations
- 2015
-
Ingår i: Journal of Physical Chemistry C. - : American Chemical Society (ACS). - 1932-7447 .- 1932-7455. ; 119:4, s. 2053-2062
-
Tidskriftsartikel (refereegranskat)abstract
- Wafer-scale surface-enhanced Raman scattering (SERS) substrates fabricated using maskless lithography are important for scalable production targets. Large-area, leaning silver-capped silicon nanopillar (Ag NP) structures suitable for SERS molecular detection at extremely low analyte concentrations are investigated. Theoretical results show that isolated Ag NPs essentially support two localized surface plasmon (LSP) modes. The most prominent LSP resonance is observed in the near-infrared region (similar to 800 nm) and can be tuned by changing the diameter of the silicon nanopillars (Si NPs). The corresponding electric field distribution maps indicate that the maximum E-field enhancement is found at the Ag cavity, i.e., the bottom part of the Ag cap. We argue that the plasmon coupling between the resonant Ag cap cavities contributes most to the enhancement of the Raman signal. We experimentally evaluate these findings and show that by exposing Si NPs to an O-2-plasma the average Ag NP cluster size, and thus the overall interpillar coupling, can be systematically reduced. We show that deposition of Cr adhesion layers on Si NPs (>3 nm) introduces plasmon coupling loss to the Ag NP LSP cavity mode that significantly reduces the SERS intensity. Results also show that short exposures to the O-2-plasma and the use of 1-3 nm Cr adhesion layers are advantageous for reducing the signal background noise from Ag NPs. In addition, the influence of the Ag NP height and Ag metal thickness on SERS intensities is investigated and optimal fabrication process parameters are evaluated. Finally, the SERS spectrum from 100 pM trans-1,2-bis(4-pyridyl) ethylene (BPE) is recorded, showing distinct characteristic Raman vibrational modes. The calculated enhancement factor is of the order of 108, and the SERS signal intensity exhibits a standard deviation of around 14% (660 data points) across a 5 x 5 mm(2) surface area.
|
|
