| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Liang, Zhenyu, et al.
(författare)
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The influence factors of the stability of tailings dam based on multi-source information fusion method
- 2019
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Ingår i: Journal of Intelligent & Fuzzy Systems. - : IOS PRESS. - 1064-1246 .- 1875-8967. ; 37:3, s. 3365-3372
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Tidskriftsartikel (refereegranskat)abstract
- The BP neural network algorithm is used to construct a stable slope angle prediction model for open-pit mines, which can successfully predict the ultimate slope angle of the mine. At the same time, a multi-tool combination of Surpac, Madis, and Flac3D is used to create a numerical model for stable slope angle in mines. The model is used to test the final slope angle prediction structure for stable mines. Through a comprehensive analysis of the ore deposit model technology constructed by the series of realms, the economic parameters involved in the generation of realm boundaries are known, and specific primaries are pointed out with the error requirements of each parameter analyzed in the realm of series. A concrete solution is put forward to the issue of realm gap. From the point of view of the power and responsibility parameters, the series of realm production methods are analyzed, and the rapid generation methods of the realm of open-pit mine series are pointed out.
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| 3. |
- Lin, Yuze, et al.
(författare)
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Balanced Partnership between Donor and Acceptor Components in Nonfullerene Organic Solar Cells with > 12% Efficiency
- 2018
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Ingår i: Advanced Materials. - : WILEY-V C H VERLAG GMBH. - 0935-9648 .- 1521-4095. ; 30:16
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Tidskriftsartikel (refereegranskat)abstract
- Relative to electron donors for bulk heterojunction organic solar cells (OSCs), electron acceptors that absorb strongly in the visible and even near-infrared region are less well developed, which hinders the further development of OSCs. Fullerenes as traditional electron acceptors have relatively weak visible absorption and limited electronic tunability, which constrains the optical and electronic properties required of the donor. Here, high-performance fullerene-free OSCs based on a combination of a medium-bandgap polymer donor (FTAZ) and a narrow-bandgap nonfullerene acceptor (IDIC), which exhibit complementary absorption, matched energy levels, and blend with pure phases on the exciton diffusion length scale, are reported. The single-junction OSCs based on the FTAZ:IDIC blend exhibit power conversion efficiencies up to 12.5% with a certified value of 12.14%. Transient absorption spectroscopy reveals that exciting either the donor or the acceptor component efficiently generates mobile charges, which do not suffer from recombination to triplet states. Balancing photocurrent generation between the donor and nonfullerene acceptor removes undesirable constraints on the donor imposed by fullerene derivatives, opening a new avenue toward even higher efficiency for OSCs.
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