| 1. |
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| 2. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 3. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 4. |
- Kanoni, Stavroula, et al.
(författare)
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Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis.
- 2022
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Ingår i: Genome biology. - : Springer Science and Business Media LLC. - 1474-760X .- 1465-6906 .- 1474-7596. ; 23:1
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Tidskriftsartikel (refereegranskat)abstract
- Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery.To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (N=1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3-5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism.Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk.
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| 5. |
- Kristan, Matej, et al.
(författare)
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The first visual object tracking segmentation VOTS2023 challenge results
- 2023
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Ingår i: 2023 IEEE/CVF International conference on computer vision workshops (ICCVW). - : Institute of Electrical and Electronics Engineers Inc.. - 9798350307443 - 9798350307450 ; , s. 1788-1810
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking Segmentation VOTS2023 challenge is the eleventh annual tracker benchmarking activity of the VOT initiative. This challenge is the first to merge short-term and long-term as well as single-target and multiple-target tracking with segmentation masks as the only target location specification. A new dataset was created; the ground truth has been withheld to prevent overfitting. New performance measures and evaluation protocols have been created along with a new toolkit and an evaluation server. Results of the presented 47 trackers indicate that modern tracking frameworks are well-suited to deal with convergence of short-term and long-term tracking and that multiple and single target tracking can be considered a single problem. A leaderboard, with participating trackers details, the source code, the datasets, and the evaluation kit are publicly available at the challenge website1
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| 6. |
- Chen, Fu qiang, et al.
(författare)
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Turbulent compressible flow analysis on multi-stage high pressure reducing valve
- 2018
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Ingår i: Flow Measurement and Instrumentation. - : Elsevier BV. - 0955-5986. ; 61, s. 26-37
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Tidskriftsartikel (refereegranskat)abstract
- Pressure reducing valve plays an important role in thermodynamic systems. Under extreme operating conditions, greater demands are requested on pressure reducing systems. In this paper, a novel multi-stage high pressure reducing valve (MSHPRV) is proposed, which can achieve multi-stage pressure reducing processes, improve the flow characteristics and deal with complex conditions. Here, the effects of different structural parameters on turbulent compressible flow inside MSHPRV are numerically investigated to achieve low valve noise and energy consumption. Mach number is taken as the parameter to reflect the fluid compressibility. Higher Mach number can cause serious aerodynamic noise and large amount of energy consumption. Based on this, transmission loss of MSHPRV is also studied to achieve better noise control performances. Meanwhile, larger turbulent dissipation rate means larger degree of energy consumption, so it is with the exergy loss. Thus, numerical models with different valve openings, perforated plate diameters, chamfer radii of perforated plates, pressure ratios and stages of perforated plates are established, and the effects of these structural parameters on the compressible turbulent flow and energy consumption of MSHPRV are investigated. Results show that different structural parameters have significant impacts on compressible turbulent flow and energy consumption performance in MSHPRV. The best noise control and least energy consumption of MSHPRV is achieved with such parameters as pressure ratio 7, perforated plate diameter 4 mm and 4 stage plates. This work can benefit the further research work on energy saving and multi-stage design of pressure reducing devices.
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| 7. |
- Xia, Xinxin, et al.
(författare)
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Revealing the crystalline packing structure of Y6 in the active layer of organic solar cells: the critical role of solvent additives
- 2023
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Ingår i: Journal of Materials Chemistry A. - : ROYAL SOC CHEMISTRY. - 2050-7488 .- 2050-7496. ; 11:40, s. 21895-21907
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Tidskriftsartikel (refereegranskat)abstract
- The bulk heterojunction (BHJ) morphology of photovoltaic materials is crucial to the fundamental optoelectronic properties of organic solar cells (OSCs). However, in the photoactive layer, the intrinsic crystalline packing structure of Y6, currently the hallmark molecule among Y-series non-fullerene acceptors (NFAs), has not been unambiguously determined. Here, employing grazing-incidence wide-angle X-ray scattering (GIWAXS), we managed to uncover the intrinsic crystalline packing structure of Y6 in the BHJ active layer of OSCs, which is found to be different from its single-crystal structure reported previously. Moreover, we find that solvent additive 1-chloronaphthalene (CN) can induce highly ordered packing of Y6 in BHJ thin films. With the help of atomistic molecular dynamics simulations, it is revealed that pi-pi interactions generally exist between naphthalene derivatives and IC terminals of Y6 analogues, which would essentially improve their long-range ordering. Our work reveals the intrinsic crystalline packing structure of Y6 in the BHJ active layer as well as its crystallization mechanism in thin films, thus providing direct correlations between this crystalline packing and the device characteristics and photophysical properties.
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| 8. |
- Chen, Min Rui, et al.
(författare)
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Effects of nanoparticles on hydraulic cavitation
- 2018
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Ingår i: XI International Conference on Computational Heat, Mass and Momentum Transfer (ICCHMT 2018). - : EDP Sciences. ; 240
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Konferensbidrag (refereegranskat)abstract
- When liquids flowing through a throttling element, such as a perforated plate, the velocity increases and the pressure decreases. If the pressure is below the saturated vapor pressure, the liquid will vaporize into small bubbles, which is called hydraulic cavitation. In fact, vaporization nucleus is another crucial condition for vaporizing. The nanoparticles contained in the nanofluids play a significant role in vaporization of liquids. In this paper, the effects of the nanoparticles on hydraulic cavitation are investigated. Firstly, a geometric model of a pipe channel equipped with a perforated plate is established. Then with different nanoparticle volume fractions and diameters, the nanofluids flowing through the channel is numerically simulated based on a validated numerical method. The operation conditions, such as the temperature and the pressure ratio of inlet to outlet, are the considered variables. As a significant parameter, cavitation numbers under different operation conditions are achieved to investigate the effects of nanoparticles on hydraulic cavitation. Meanwhile, the contours are extracted to research the distribution of bubbles for further investigation. This study is of interests for researchers working on hydraulic cavitation or nanofluids.
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| 9. |
- Chen, Min Rui, et al.
(författare)
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The hydraulic cavitation affected by nanoparticles in nanofluids
- 2018
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Ingår i: Computation. - : MDPI AG. - 2079-3197. ; 6:3
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Tidskriftsartikel (refereegranskat)abstract
- When liquids flow through a throttling element, the velocity increases and the pressure decreases. At this point, if the pressure is below the saturated vapor pressure of this liquid, the liquid will vaporize into small bubbles, causing hydraulic cavitation. In fact, a vaporization nucleus is another crucial condition for vaporizing, and particles contained in the liquid can also work as the vaporization nuclear. As a novel heat transfer medium, nanofluids have attracted the attention of many scholars. The nanoparticles contained in the nanofluids play a significant role in the vaporization of liquids. In this paper, the effects of the nanoparticles on hydraulic cavitation are investigated. Firstly, a geometric model of a perforated plate, the throttling element in this paper, is established. Then with different nanoparticle volume fractions and diameters, the nanofluids flowing through the perforated plate are numerically simulated based on a validated numerical method. The operation conditions, such as the ratio of inlet to outlet pressures and the temperature are the considered variables. Additionally, cavitation numbers under different operating conditions are achieved to investigate the effects of nanoparticles on hydraulic cavitation. Meanwhile, the contours are extracted to research the distribution of bubbles for further investigation. This study is of interest for researchers working on hydraulic cavitation or nanofluids.
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| 10. |
- Chen, Zhi, et al.
(författare)
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Large-Area Crystalline Zeolitic Imidazolate Framework Thin Films
- 2021
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Ingår i: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 60:25, s. 14124-14130
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Tidskriftsartikel (refereegranskat)abstract
- We report that continuous MOF films with highly controlled thickness (from 44 to 5100 nm) can be deposited over length scales greater than 80 centimeters by a facile, fast, and cost-effective spray-coating method. Such success relies on our discovery of unprecedented perfectly dispersed colloidal solutions consisting of amorphous MOF nanoparticles, which we adopted as precursors that readily converted to the crystalline films upon low-temperature in situ heating. The colloidal solutions allow for the fabrication of compact and uniform MOF films on a great deal of substrates such as fluorine-doped tin oxide, glass, SiO2, Al2O3, Si, Cu, and even flexible polycarbonate, widening their technological applications where substrates are essential. Despite the present work focuses on the fabrication of uniform cobalt-(2-methylimidazole)2 and zinc-(2-methylimidazole)2 films, our findings mark a great possibility in producing other high-quality MOF thin films on a large scale.
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| 11. |
- Du, Mulong, et al.
(författare)
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Cyp2a6 activity and cigarette consumption interact in smoking-related lung cancer susceptibility
- 2024
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Ingår i: Cancer Research. - : American Association For Cancer Research (AACR). - 0008-5472 .- 1538-7445. ; 84:4, s. 616-625
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Tidskriftsartikel (refereegranskat)abstract
- Cigarette smoke, containing both nicotine and carcinogens, causes lung cancer. However, not all smokers develop lung cancer, highlighting the importance of the interaction between host susceptibility and environmental exposure in tumorigenesis. Here, we aimed to delineate the interaction between metabolizing ability of tobacco carcinogens and smoking intensity in mediating genetic susceptibility to smoking-related lung tumorigenesis. Single-variant and gene-based associations of 43 tobacco carcinogen–metabolizing genes with lung cancer were analyzed using summary statistics and individual-level genetic data, followed by causal inference of Mendelian randomization, mediation analysis, and structural equation modeling. Cigarette smoke–exposed cell models were used to detect gene expression patterns in relation to specific alleles. Data from the International Lung Cancer Consortium (29,266 cases and 56,450 controls) and UK Biobank (2,155 cases and 376,329 controls) indicated that the genetic variant rs56113850 C>T located in intron 4 of CYP2A6 was significantly associated with decreased lung cancer risk among smokers (OR = 0.88, 95% confidence interval = 0.85–0.91, P = 2.18 X 10-16), which might interact (Pinteraction = 0.028) with and partially be mediated (ORindirect = 0.987) by smoking status. Smoking intensity accounted for 82.3% of the effect of CYP2A6 activity on lung cancer risk but entirely mediated the genetic effect of rs56113850. Mechanistically, the rs56113850 T allele rescued the downregulation of CYP2A6 caused by cigarette smoke exposure, potentially through preferential recruitment of transcription factor helicase-like transcription factor. Together, this study provides additional insights into the interplay between host susceptibility and carcinogen exposure in smoking-related lung tumorigenesis.
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| 12. |
- Luo, Zhenghui, et al.
(författare)
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Fine-Tuning Energy Levels via Asymmetric End Groups Enables Polymer Solar Cells with Efficiencies over 17%
- 2020
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Ingår i: Joule. - : CELL PRESS. - 2542-4351. ; 4:6, s. 1236-1247
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Tidskriftsartikel (refereegranskat)abstract
- Generally, it is important to fine-tune the energy levels of donor and acceptor materials in the field of polymer solar cell (PSCs) to achieve a minimal highest occupied molecular orbital (HOMO) energy offset, which yet is still sufficient for charge separation. Based on the high-performance small-molecule acceptor (SMA) of BTP-4F, we modified the end groups of BTP-4F from IC-2F to CPTCN-Cl. It was found that when both end groups were substituted by CPTCN-Cl, the energy level upshift was too large that caused unfavorable energetic alignment, thus poor device performance. By using the strategy of asymmetric end groups, we were able to achieve near optimal energy level match, resulting in higher open-circuit voltage (V-OC) and power conversion efficiency (PCE) compared with those given by the PM6:BTP-4F system. Our strategy can be useful and potentially applied to othermaterial systems for maximizing efficiency of non-fullerene PSCs.
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| 13. |
- Qian, Jin yuan, et al.
(författare)
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A numerical study of heat transfer effects and aerodynamic noise reduction in superheated steam flow passing a temperature and pressure regulation valve
- 2020
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Ingår i: Numerical Heat Transfer; Part A: Applications. - : Informa UK Limited. - 1040-7782 .- 1521-0634. ; 77:10, s. 873-889
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Tidskriftsartikel (refereegranskat)abstract
- Steam is a common medium in thermal engineering. When it flows through a throttling element, the aerodynamic noise may occur due to the disturbance. In this investigation, superheated steam flowing through a Venturi tube, one of the main parts in a temperature and pressure regulation valve, at different thermal conditions is studied to analyze to effects of heat transfer on the acoustic power. With a high temperature and a low pressure, the superheated steam is treated as ideal gas. The flow velocity is high, so the k-epsilon turbulent model is used, with the compressible steam. The results show that under the adiabatic condition, the acoustic power mainly influenced by the turbulent characteristics, such as the dissipation rate and the turbulent kinetic energy. Comparing the acoustic power levels at different thermal conditions, it is found that a lower temperature results to a lower acoustic power.
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| 14. |
- Yang, Chen, et al.
(författare)
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Heat transfer study on a hybrid smooth and spirally corrugated tube
- 2018
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Ingår i: XI International Conference on Computational Heat, Mass and Momentum Transfer (ICCHMT 2018). - : EDP Sciences. ; 240
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Konferensbidrag (refereegranskat)abstract
- Corrugated tubes are widely used in a range of applications for heat transfer enhancement. The spirally corrugated tube has a better heat transfer performance than the smooth tube. In this paper, the heat transfer performance of a hybrid smooth and six-start spirally corrugated tube is studied. With a validated numerical model, the effects of the corrugation part length on the vortex in the downstream smooth tube are studied for a range of high Reynolds numbers, where the existence of the corrugation part can turn out the secondary flow and enhance heat transfer. Meanwhile, it is found that in the smooth part, the fluid flow part with whirling can reach a maximum length, even if the length of the corrugation part continuously increases. Thus a series of critical corrugation lengths can be obtained. This work can reveal the enhanced heat transfer mechanism of the hybrid smooth and spirally corrugated tube and be of interest to researchers in heat transfer issues of corrugated tubes.
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| 15. |
- Zheng, Hou-Feng, et al.
(författare)
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Whole-genome sequencing identifies EN1 as a determinant of bone density and fracture
- 2015
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 526:7571, s. 112-
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Tidskriftsartikel (refereegranskat)abstract
- The extent to which low-frequency (minor allele frequency (MAF) between 1-5%) and rare (MAF <= 1%) variants contribute to complex traits and disease in the general population is mainly unknown. Bone mineral density (BMD) is highly heritable, a major predictor of osteoporotic fractures, and has been previously associated with common genetic variants(1-8), as well as rare, population specific, coding variants(9). Here we identify novel non-coding genetic variants with large effects on BMD (n(total) = 53,236) and fracture (n(total) = 508,253) in individuals of European ancestry from the general population. Associations for BMD were derived from whole-genome sequencing (n = 2,882 from UK10K (ref. 10); a population-based genome sequencing consortium), whole-exome sequencing (n = 3,549), deep imputation of genotyped samples using a combined UK10K/1000 Genomes reference panel (n = 26,534), and de novo replication genotyping (n = 20,271). We identified a low-frequency non-coding variant near a novel locus, EN1, with an effect size fourfold larger than the mean of previously reported common variants for lumbar spine BMD8 (rs11692564(T), MAF51.6%, replication effect size510.20 s.d., P-meta = 2 x 10(-14)), which was also associated with a decreased risk of fracture (odds ratio = 0.85; P = 2 x 10(-11); ncases = 98,742 and ncontrols = 409,511). Using an En1cre/flox mouse model, we observed that conditional loss of En1 results in low bone mass, probably as a consequence of high bone turnover. We also identified a novel low frequency non-coding variant with large effects on BMD near WNT16 (rs148771817(T), MAF = 1.2%, replication effect size +10.41 s.d., P-meta = 1 x 10(-11)). In general, there was an excess of association signals arising from deleterious coding and conserved non-coding variants. These findings provide evidence that low-frequency non-coding variants have large effects on BMD and fracture, thereby providing rationale for whole-genome sequencing and improved imputation reference panels to study the genetic architecture of complex traits and disease in the general population.
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| 16. |
- Abolfathi, Bela, et al.
(författare)
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The Fourteenth Data Release of the Sloan Digital Sky Survey : First Spectroscopic Data from the Extended Baryon Oscillation Spectroscopic Survey and from the Second Phase of the Apache Point Observatory Galactic Evolution Experiment
- 2018
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Ingår i: Astrophysical Journal Supplement Series. - : IOP Publishing Ltd. - 0067-0049 .- 1538-4365. ; 235:2
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Tidskriftsartikel (refereegranskat)abstract
- The fourth generation of the Sloan Digital Sky Survey (SDSS-IV) has been in operation since 2014 July. This paper describes the second data release from this phase, and the 14th from SDSS overall (making this Data Release Fourteen or DR14). This release makes the data taken by SDSS-IV in its first two years of operation (2014-2016 July) public. Like all previous SDSS releases, DR14 is cumulative, including the most recent reductions and calibrations of all data taken by SDSS since the first phase began operations in 2000. New in DR14 is the first public release of data from the extended Baryon Oscillation Spectroscopic Survey; the first data from the second phase of the Apache Point Observatory (APO) Galactic Evolution Experiment (APOGEE-2), including stellar parameter estimates from an innovative data-driven machine-learning algorithm known as "The Cannon"; and almost twice as many data cubes from the Mapping Nearby Galaxies at APO (MaNGA) survey as were in the previous release (N = 2812 in total). This paper describes the location and format of the publicly available data from the SDSS-IV surveys. We provide references to the important technical papers describing how these data have been taken (both targeting and observation details) and processed for scientific use. The SDSS web site (www.sdss.org) has been updated for this release and provides links to data downloads, as well as tutorials and examples of data use. SDSS-IV is planning to continue to collect astronomical data until 2020 and will be followed by SDSS-V.
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| 17. |
- Chen, Min Rui, et al.
(författare)
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Numerical Simulation of Droplet Generation in Cross-Junction Microchannel under Changing Dispersion Phase Velocities
- 2018
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Ingår i: Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities. - 1003-9015. ; 32:3, s. 522-528
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Tidskriftsartikel (refereegranskat)abstract
- Micro-droplets with excellent physical properties and size effects are widely used in different fields. Peristaltic pumps are often used for droplet generation. The thrust force of pumps is periodic as a periodic disturbance when the time scale is short enough. Numerical simulation based on a validated model was applied to study the periodic disturbance effects on droplet generation in a cross-junction microchannel. A volume of fluid (VOF) method was adopted with the continuous phase of silicon oil and the disperse phase of water. When the velocity of the continuous and dispersed phases is both 0.01 m∙s-1, the droplet generation time is 0.08 s. Sine functions with cycles of 0.04, 0.08, 0.12 and 0.16 s and amplitudes of 0.0025, 0.005, 0.0075 and 0.01 were imported as the inlet velocity of the disperse phase. Droplet sizes and generation times were obtained by simulating different flow processes. The results show that when the cycle is less than 0.08 s, changes of cycle time and amplitude have few effects on droplet size and generation time. However, when the cycle time is larger than 0.08 s, droplet size and generation time change periodically. Furthermore, amplitude has few effects on droplet generation.
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| 18. |
- Chen, Ying, et al.
(författare)
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Enhanced colonic tumorigenesis in alkaline sphingomyelinase (NPP7) knockout mice.
- 2015
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Ingår i: Molecular Cancer Therapeutics. - 1538-8514. ; 14:1, s. 259-267
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Tidskriftsartikel (refereegranskat)abstract
- Intestinal alkaline sphingomyelinase (alk-SMase) generates ceramide and inactivates platelet-activating factor (PAF) and is previously suggested to have anticancer properties. The direct evidence is still lacking. We studied colonic tumorigenesis in alk-SMase knockout (KO) mice. Formation of aberrant crypt foci (ACF) was examined after azoxymethane (AOM) injection. Tumor was induced by AOM alone, a conventional AOM/dextran sulfate sodium (DSS) treatment, and an enhanced AOM/DSS method. beta-catenin was determined by immunohistochemistry, PAF levels by ELISA and sphingomyelin metabolites by mass spectrometry. Without treatment, spontaneous tumorigenesis was not identified but the intestinal mucosa appeared thicker in KO than in wild type (WT) littermates. AOM alone induced more ACF in KO mice but no tumors 28 weeks after injection. However, combination of AOM/DSS treatments induced colonic tumors and the incidence was significantly higher in KO than in WT mice. By the enhanced AOM/DSS method tumor number per mouse increased 4.5 times and tumor size 1.8 times in KO compared to WT mice. While all tumors were adenomas in WT mice, 32% were adenocarcinomas in KO mice. Compared to WT mice, cytosol expression of beta-catenin was significantly decreased and nuclear translocation in tumors was more pronounced in KO mice. Lipid analysis showed decreased ceramide in small intestine and increased sphingosine-1-phosphate in both small intestine and colon in nontreated KO mice. PAF levels in feces were significantly higher in the KO mice after AOM/DSS treatment. In conclusion lack of alk-SMase markedly increases AOM/DSS induced colonic tumorigenesis associated with decreased ceramide and increased sphingosine-1-phosphate and PAF levels.
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| 19. |
- Feng, Ruizhi, et al.
(författare)
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Mutations in TUBB8 and Human Oocyte Meiotic Arrest.
- 2016
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Ingår i: The New England journal of medicine. - 1533-4406. ; 374:3, s. 223-232
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Tidskriftsartikel (refereegranskat)abstract
- Background Human reproduction depends on the fusion of a mature oocyte with a sperm cell to form a fertilized egg. The genetic events that lead to the arrest of human oocyte maturation are unknown. Methods We sequenced the exomes of five members of a four-generation family, three of whom had infertility due to oocyte meiosis I arrest. We performed Sanger sequencing of a candidate gene, TUBB8, in DNA samples from these members, additional family members, and members of 23 other affected families. The expression of TUBB8 and all other β-tubulin isotypes was assessed in human oocytes, early embryos, sperm cells, and several somatic tissues by means of a quantitative reverse-transcriptase-polymerase-chain-reaction assay. We evaluated the effect of the TUBB8 mutations on the assembly of the heterodimer consisting of one α-tubulin polypeptide and one β-tubulin polypeptide (α/β-tubulin heterodimer) in vitro, on microtubule architecture in HeLa cells, on microtubule dynamics in yeast cells, and on spindle assembly in mouse and human oocytes. Results We identified seven mutations in the primate-specific gene TUBB8 that were responsible for oocyte meiosis I arrest in 7 of the 24 families. TUBB8 expression is unique to oocytes and the early embryo, in which this gene accounts for almost all the expressed β-tubulin. The mutations affect chaperone-dependent folding and assembly of the α/β-tubulin heterodimer, disrupt microtubule behavior on expression in cultured cells, alter microtubule dynamics in vivo, and cause catastrophic spindle-assembly defects and maturation arrest on expression in mouse and human oocytes. Conclusions TUBB8 mutations have dominant-negative effects that disrupt microtubule behavior and oocyte meiotic spindle assembly and maturation, causing female infertility. (Funded by the National Basic Research Program of China and others.).
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| 20. |
- Li, Xiao Juan, et al.
(författare)
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Research progress of frost formation on microchannel heat exchangers
- 2017
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Ingår i: Xiandai Huagong/Modern Chemical Industry. - 0253-4320. ; 37:11, s. 47-52
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Forskningsöversikt (refereegranskat)abstract
- Focusing on the frost formation in microchannel heat exchangers, this paper analyzes the effects of frost formation on the thermal performance of microchannel heat exchangers, and summarizes the influence factors that cause the microchannel heat exchangers to form frost, including environmental factors and structure factors. The latest research progress in this issue at home and abroad is reviewed.
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| 21. |
- Qian, Jin Yuan, et al.
(författare)
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A geometric study on shell side heat transfer and flow resistance of a six-start spirally corrugated tube
- 2018
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Ingår i: Numerical Heat Transfer; Part A: Applications. - : Informa UK Limited. - 1040-7782 .- 1521-0634. ; 73:8, s. 565-582
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Tidskriftsartikel (refereegranskat)abstract
- Heat transfer enhancement is of great importance for energy efficiency improvement. The utilization of spirally corrugated tubes is one of the efficient ways to strengthen heat transfer. In this article, based on a validated numerical model, the effects of geometric parameters of a six-start spirally corrugated tube, including the pitch p and the corrugation depth e, on the shell side heat transfer and flow resistance performance are numerically investigated, in high Reynolds number conditions ranging from 10,000 to 60,000. The shell side secondary flow velocity distribution, longitudinal vortex distribution, and temperature distribution of a six-start spirally corrugated tube are presented, respectively. In addition, the heat transfer and flow resistance characteristics are evaluated by comparing the Nusselt number and the flow resistance coefficient with these of smooth tubes. Results show that the utilization of six-start spirally corrugated tubes can enhance the heat transfer performance at the expense of an increase of the flow resistance. However, with the same geometric parameters, the Nusselt number increases and the flow resistance coefficient decreases as Reynolds number increases. With the pitch increasing, the Nusselt number and the flow resistance coefficient decrease at a fixed Reynolds number. In contrast, as the corrugation depth increasing, the Nusselt number changes irregularly, and the flow resistance coefficient increases. Finally, correlations for the shell side Nusselt number and flow resistance coefficient of the six-start spirally corrugated tube are established. This work is of significance for engineers and scientists focusing on the heat transfer and the flow resistance characteristics of spirally corrugated tubes and their applications.
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| 22. |
- Qian, Jin Yuan, et al.
(författare)
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Effects of a dynamic injection flow rate on slug generation in a cross-junction square microchannel
- 2019
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Ingår i: Processes. - : MDPI AG. - 2227-9717. ; 7:10
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Tidskriftsartikel (refereegranskat)abstract
- The injection flow rates of two liquid phases play a decisive role in the slug generation of the liquid-liquid slug flow. However, most injection flow rates so far have been constant. In order to investigate the effects of dynamic injection flow rates on the slug generation, including the slug size, separation distance and slug generation cycle time, a transient numerical model of a cross-junction square microchannel is established. The Volume of Fluid method is adopted to simulate the interface between two phases, i.e., butanol and water. The model is validated by experiments at a constant injection flow rate. Three different types of dynamic injection flow rates are applied for butanol, which are triangle, rectangular and sine wave flow rates. The dynamic injection flow rate cycles, which are related to the constant slug generation cycle time t0, are investigated. Results show that when the cycle of the disperse phase flow rate is larger than t0, the slug generation changes periodically, and the period is influenced by the cycle of the disperse phase flow rate. Among the three kinds of dynamic disperse flow rate, the rectangular wave influences the slug size most significantly, while the triangle wave influences the separation distance and the slug generation time more prominently.
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| 23. |
- Qian, Jin Yuan, et al.
(författare)
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Pressure fluctuations of liquid-liquid slug flow in cross-junction square microchannels
- 2018
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Ingår i: Proceeding: International Heat Transfer Conference 16. - 2377-424X. ; , s. 7231-7241
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Konferensbidrag (refereegranskat)abstract
- Microchannels are widely used for heat transfer enhancement. The pressure characteristics are one of the most important factors affecting the heat transfer performance, and the pressure regulation may also control the heat transfer precisely in turn. As a common flow pattern of two-phase flow, slug flow has obvious advantages for heat and mass transfer, like steady flow rate and large interface area etc. Due to the interface stress and the velocity difference between the continuous phase and the dispersed phase, pressure fluctuations occur in both the flowing direction and the cross section. In oil and natural gas industries, pressure fluctuations of slug flow can be used for the slug size and velocity measurement, and also to analyze the principle of slug generation. However, when it comes to micro scale, pressure fluctuations of slug flow in microchannels is difficult to measure, not only due to the pressure difference within a low pressure range (10 to 103 Pa), but also because of the small size, for which conventional pressure sensors cannot be utilized. In this paper, a numerical method is adopted for the liquid-liquid slug flow (butanol/water) pressure prediction in a cross-junction square microchannel. To begin with, the validation of the numerical method is carried out by comparing the slug size with experiments under the same working conditions. Then, both pressure fluctuations in the flow direction and in the cross section are investigated. With a transient flow model, pressure fluctuations in the cross section at different flowing time are observed. Finally, effects of the dispersed phase (butanol) injection velocity on pressure fluctuations are performed. This work can be used for further study of the slug generation in microchannels.
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| 24. |
- Qian, Zhen, et al.
(författare)
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Vectorized dataset of roadside noise barriers in China using street view imagery
- 2022
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Ingår i: Earth System Science Data. - : Copernicus GmbH. - 1866-3508 .- 1866-3516. ; 14:9, s. 4057-4076
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Tidskriftsartikel (refereegranskat)abstract
- Roadside noise barriers (RNBs) are important urban infrastructures to ensure that cities remain liveable. However, the absence of accurate and large-scale geospatial data on RNBs has impeded the increasing progress of rational urban planning, sustainable cities, and healthy environments. To address this problem, this study creates a vectorized RNB dataset in China using street view imagery and a geospatial artificial intelligence framework. First, intensive sampling is performed on the road network of each city based on OpenStreetMap, which is used as the georeference for downloading 6 x 10(6) Baidu Street View (BSV) images. Furthermore, considering the prior geographic knowledge contained in street view images, convolutional neural networks incorporating image context information (IC-CNNs) based on an ensemble learning strategy are developed to detect RNBs from the BSV images. The RNB dataset presented by polylines is generated based on the identified RNB locations, with a total length of 2667.02 km in 222 cities. Last, the quality of the RNB dataset is evaluated from two perspectives, i.e., the detection accuracy and the completeness and positional accuracy. Specifically, based on a set of randomly selected samples containing 10 000 BSV images, four quantitative metrics are calculated, with an overall accuracy of 98.61 %, recall of 87.14 %, precision of 76.44 %, and F-1 score of 81.44 %. A total length of 254.45 km of roads in different cities are manually surveyed using BSV images to evaluate the mileage deviation and overlap level between the generated and surveyed RNBs. The root mean squared error for the mileage deviation is 0.08 km, and the intersection over union for overlay level is 88.08% +/- 2.95 %. The evaluation results suggest that the generated RNB dataset is of high quality and can be applied as an accurate and reliable dataset for a variety of large-scale urban studies, such as estimating the regional solar photovoltaic potential, developing 3D urban models, and designing rational urban layouts. Besides that, the benchmark dataset of the labeled BSV images can also support more work on RNB detection, such as developing more advanced deep learning algorithms, fine-tuning the existing computer vision models, and analyzing geospatial scenes in BSV. The generated vectorized RNB dataset and the benchmark dataset of labeled BSV imagery are publicly available at https://doi.org/10.11888/Others.tpdc.271914 (Chen, 2021).
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| 25. |
- Senanayake, Indunil C., et al.
(författare)
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Fungal diversity notes 1611–1716: taxonomic and phylogenetic contributions on fungal genera and species emphasis in south China
- 2023
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Ingår i: Fungal Diversity. - 1560-2745 .- 1878-9129. ; 122, s. 161-403
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Tidskriftsartikel (refereegranskat)abstract
- This article is the 15th contribution in the Fungal Diversity Notes series, wherein 115 taxa from three phyla, nine classes, 28 orders, 48 families, and 64 genera are treated. Fungal taxa described and illustrated in the present study include a new family, five new genera, 61 new species, five new combinations, one synonym, one new variety and 31 records on new hosts or new geographical distributions. Ageratinicolaceae fam. nov. is introduced and accommodated in Pleosporales. The new genera introduced in this study are Ageratinicola, Kevinia, Pseudomultiseptospora (Parabambusicolaceae), Marasmiellomycena, and Vizzinia (Porotheleaceae). Newly described species are Abrothallus altoandinus, Ageratinicola kunmingensis, Allocryptovalsa aceris, Allophoma yuccae, Apiospora cannae, A. elliptica, A. pallidesporae, Boeremia wisteriae, Calycina papaeana, Clypeococcum lichenostigmoides, Coniochaeta riskali-shoyakubovii, Cryphonectria kunmingensis, Diaporthe angustiapiculata, D. campylandrae, D. longipapillata, Diatrypella guangdongense, Dothiorella franceschinii, Endocalyx phoenicis, Epicoccum terminosporum, Fulvifomes karaiensis, F. pannaensis, Ganoderma ghatensis, Hysterobrevium baoshanense, Inocybe avellaneorosea, I. lucida, Jahnula oblonga, Kevinia lignicola, Kirschsteiniothelia guangdongensis, Laboulbenia caprina, L. clavulata, L. cobiae, L. cosmodisci, L. nilotica, L. omalii, L. robusta, L. similis, L. stigmatophora, Laccaria rubriporus, Lasiodiplodia morindae, Lyophyllum agnijum, Marasmiellomycena pseudoomphaliiformis, Melomastia beihaiensis, Nemania guangdongensis, Nigrograna thailandica, Nigrospora ficuum, Oxydothis chinensis, O. yunnanensis, Petriella thailandica, Phaeoacremonium chinensis, Phialocephala chinensis, Phytophthora debattistii, Polyplosphaeria nigrospora, Pronectria loweniae, Seriascoma acutispora, Setoseptoria bambusae, Stictis anomianthi, Tarzetta tibetensis, Tarzetta urceolata, Tetraploa obpyriformis, Trichoglossum beninense, and Tricoderma pyrrosiae. We provide an emendation for Urnula ailaoshanensis Agaricus duplocingulatoides var. brevisporus introduced as a new variety based on morphology and phylogeny.
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| 26. |
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| 27. |
- Yu, Han, et al.
(författare)
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Fluorinated End Group Enables High-Performance All-Polymer Solar Cells with Near-Infrared Absorption and Enhanced Device Efficiency over 14%
- 2021
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Ingår i: Advanced Energy Materials. - : WILEY-V C H VERLAG GMBH. - 1614-6832 .- 1614-6840. ; 11:4
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Tidskriftsartikel (refereegranskat)abstract
- Fluorination of end groups has been a great success in developing efficient small molecule acceptors. However, this strategy has not been applied to the development of polymer acceptors. Here, a dihalogenated end group modified by fluorine and bromine atoms simultaneously, namely IC-FBr, is first developed, then employed to construct a new polymer acceptor (named PYF-T) for all-polymer solar cells (all-PSCs). In comparison with its non-fluorinated counterpart (PY-T), PYF-T exhibits stronger and red-shifted absorption spectra, stronger molecular packing and higher electron mobility. Meanwhile, the fluorination on the end groups down-shifts the energy levels of PYF-T, which matches better with the donor polymer PM6, leading to efficient charge transfer and small voltage loss. As a result, an all-PSC based on PM6:PYF-T yields a higher power conversion efficiency (PCE) of 14.1% than that of PM6:PY-T (11.1%), which is among the highest values for all-PSCs reported to date. This work demonstrates the effectiveness of fluorination of end-groups in designing high-performance polymer acceptors, which paves the way toward developing more efficient and stable all-PSCs.
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| 28. |
- Zhang, Kai, et al.
(författare)
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Integrating photovoltaic noise barriers and electric vehicle charging stations for sustainable city transportation
- 2024
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Ingår i: Sustainable cities and society. - 2210-6707. ; 100
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Tidskriftsartikel (refereegranskat)abstract
- Photovoltaic noise barriers (PVNBs) offer a dual advantage of reducing traffic noise pollution and providing renewable electricity to cities. However, how the effective integration of PVNB-generated power into urban energy networks remains a critical area lacking research. To bridge this gap, this study proposes PVNBs-energy storage (ES)-charging station (CS; PVNBs-ES-CS) strategy. It can facilitate the actual consumption of PVNBs power and the mitigation the burden on the grid posed by electric vehicles (EVs) charging demands. The case study conducted in Guangzhou, China, reveals that PVNBs can support up to 5% of the total power demand for EVCSs. Under the PVNBs power maximization consumption scenario, PVNBs can meet up to 30% of the power demands from 60 EVCSs, with 58% of PVNBs generated power being consumed. In the PVNBs-ES-CS future utilization scenario, up to 30% of the power demand of 125 EVCSs can be met, and 36% of the power of PVNBs can be consumed. The combination of PVNBs and EVCSs offers a practical solution for incorporating renewable energy sources into urban energy networks. This application mode can be applied in various cities with EV demands and PVNB power generation data.
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| 29. |
- Zhang, Kai, et al.
(författare)
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Quantifying the photovoltaic potential of highways in China
- 2022
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Ingår i: Applied Energy. - : Elsevier BV. - 0306-2619 .- 1872-9118. ; 324, s. 119600-
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Tidskriftsartikel (refereegranskat)abstract
- Installing photovoltaic (PV) modules on highways is considered a promising way to support carbon neutrality in China. However, collecting the area of the highway, and precisely assessing the shadow area of the highway under complex terrain remain challenges. That severely hinders the assessment of highway PV potential. To address these challenges, a spatiotemporal model is developed in this study to estimate the annual solar PV potential on highways over the whole Chinese territory. First, the areas of different highway segments are calculated based on highway network and highway toll stations. Second, hourly shadow area on highways created by nearby terrain is estimated based on a digital elevation model (DEM). When calculating the highway PV potential, the solar irradiation received in these shadow areas is regarded as zero. Finally, the PV potential of all lanes and emergency lanes was estimated at the prefecture-level city scale using surface radiation data and radiation assessment models. Based on the highway data with a total mileage of 143,684 km at the end of 2020, the results show that the annual PV potential is 3,932 TW and that the corresponding installed capacity is 700.85 GW, which can generate clean electricity at a rate of up to 629.06 TWh. The annual PV potential of highways in the southeast is greater than that in the northwest owing to the higher highway density in the southeast. This study provides a reference basis for highway PV construction planning and suitably assessment in each region of China for PV highway development.
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| 30. |
- Zhang, Lu, et al.
(författare)
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Toward terabit digital radio over fiber systems : Architecture and key technologies
- 2019
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Ingår i: IEEE Communications Magazine. - : Institute of Electrical and Electronics Engineers Inc.. - 0163-6804 .- 1558-1896. ; 57:4, s. 131-137
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Tidskriftsartikel (refereegranskat)abstract
- To support massive deployment of broadband radio applications, such as 5G and high-definition videos for terrestrial televisions, large system capacity and high spectrum efficiency are highly demanded in radio over fiber (RoF) systems. In this article, we propose a terabit digital RoF system capable of providing high-speed transmission, where multicore fiber (MCF) is introduced for the access segment between the central unit and remote unit. Two key technologies that greatly enhance system capacity and spectrum efficiency, namely MCF enabled self-homodyne detection and compressed quantization, are demonstrated.
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| 31. |
- Zhang, Zhixin, et al.
(författare)
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Vectorized rooftop area data for 90 cities in China
- 2022
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Ingår i: Scientific Data. - : Springer Nature. - 2052-4463. ; 9:1
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Tidskriftsartikel (refereegranskat)abstract
- Reliable information on building rooftops is crucial for utilizing limited urban space effectively. In recent decades, the demand for accurate and up-to-date data on the areas of rooftops on a large-scale is increasing. However, obtaining these data is challenging due to the limited capability of conventional computer vision methods and the high cost of 3D modeling involving aerial photogrammetry. In this study, a geospatial artificial intelligence framework is presented to obtain data for rooftops using high-resolution open-access remote sensing imagery. This framework is used to generate vectorized data for rooftops in 90 cities in China. The data was validated on test samples of 180 km(2) across different regions with spatial resolution, overall accuracy, and F1 score of 1 m, 97.95%, and 83.11%, respectively. In addition, the generated rooftop area conforms to the urban morphological characteristics and reflects urbanization level. These results demonstrate that the generated dataset can be used for data support and decision-making that can facilitate sustainable urban development effectively.
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| 32. |
- Zhong, Teng, et al.
(författare)
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A city-scale estimation of rooftop solar photovoltaic potential based on deep learning
- 2021
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Ingår i: Applied Energy. - : Elsevier BV. - 0306-2619 .- 1872-9118. ; 298
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Tidskriftsartikel (refereegranskat)abstract
- The estimation of rooftop solar photovoltaic (PV) potential is crucial for policymaking around sustainable energy plans. But it is difficult to accurately estimate the availability of rooftop area for solar radiation on a city-scale. In this study, a generic framework for estimating the rooftop solar PV potential on a city-scale using publicly available high-resolution satellite images is proposed. A deep learning-based method is developed to extract the rooftop area with image semantic segmentation automatically. A spatial optimization sampling strategy is developed to solve the labor-intensive problem when training the rooftop extraction model based on prior knowledge of urban and rural spatial layout and land use. In the case study of Nanjing, China, the labor cost on preparing the dataset for training the rooftop extraction model has been reduced by about 80% with the proposed spatial optimization sampling strategy. Meanwhile, the robustness of the rooftop extraction model in districts with different architectural styles and land use has been improved. The total rooftop area extracted was 330.36 km(2), and the overall accuracy reached 0.92. The estimation results show that Nanjing has significant potential for rooftop-mounted PV installations, and the potential installed capacity reached 66 GW. The annual rooftop solar PV potential was approximately 311,853 GWh, with a corresponding estimated power generation of 49,897 GWh in 2019.
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