| 1. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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| 2. |
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| 3. |
- Clarke, Leonardo, et al.
(författare)
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An Old Stellar Population or Diffuse Nebular Continuum Emission Discovered in Green Pea Galaxies
- 2021
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Ingår i: Astrophysical Journal Letters. - : American Astronomical Society. - 2041-8205 .- 2041-8213. ; 912:2
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Tidskriftsartikel (refereegranskat)abstract
- We use new Hubble Space Telescope (HST) images of nine Green Pea galaxies (GPGs) to study their resolved structure and color. The choice of filters, F555W and F850LP, together with the redshift of the galaxies (z ∼ 0.25), minimizes the contribution of the nebular [O iii] and Hα emission lines to the broadband images. While these galaxies are typically very blue in color, our analysis reveals that it is only the dominant stellar clusters that are blue. Each GPG does clearly show the presence of at least one bright and compact star-forming region, but these are invariably superimposed on a more extended and lower surface brightness emission. Moreover, the colors of the star-forming regions are on average bluer than those of the diffuse emission, reaching up to 0.6 magnitudes bluer. Assuming that the diffuse and compact components have constant and single-burst star formation histories, respectively, the observed colors imply that the diffuse components (possibly the host galaxy of the star formation episode) have, on average, old stellar ages (>1 Gyr), while the star clusters are younger than 500 Myr. While a redder stellar component is perhaps the most plausible explanation for these results, the limitations of our current data set lead us to examine possible alternative mechanisms, particularly recombination emission processes, which are unusually prominent in systems with such strong line emission. With the available data, however, it is not possible to distinguish between these two interpretations. A substantial presence of old stars would indicate that the mechanisms allowing large escape fractions in these local galaxies may be different from those at play during the reionization epoch.
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| 4. |
- Lin, Yu-Heng, et al.
(författare)
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Low-metallicity Galaxies from the Dark Energy Survey
- 2023
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Ingår i: Astrophysical Journal. - 0004-637X .- 1538-4357. ; 951:2
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Tidskriftsartikel (refereegranskat)abstract
- We present a new selection of 358 blue compact dwarf galaxies (BCDs) from 5000 square degrees in the Dark Energy Survey, and the spectroscopic follow-up of a subsample of 68 objects. For the subsample of 34 objects with deep spectra, we measure the metallicity via the direct Te method using the auroral [O iii]λ 4363 emission line. These BCDs have an average oxygen abundance of 12+log(O/H) = 7.8, with stellar masses between 107 and 108M⊙ and specific star-formation rates between ∼10−9 and 10−7 yr−1. We compare the position of our BCDs with the mass–metallicity (M–Z) and luminosity–metallicity (L–Z) relation derived from the Local Volume Legacy sample. We find the scatter about the M–Z relation is smaller than the scatter about the L–Z relation. We identify a correlation between the offsets from the M–Z and L–Z relation that we suggest is due to the contribution of metal-poor inflows. Finally, we explore the validity of the mass–metallicity–SFR fundamental plane in the mass range probed by our galaxies. We find that BCDs with stellar masses smaller than 108M⊙ do not follow the extrapolation of the fundamental plane. This result suggests that mechanisms other than the balance between inflows and outflows may be at play in regulating the position of low-mass galaxies in the M–Z–SFR space.
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| 5. |
- Aad, G, et al.
(författare)
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- 2015
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swepub:Mat__t
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