| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Deng, Min, et al.
(författare)
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Genome-wide association analyses in Han Chinese identify two new susceptibility loci for amyotrophic lateral sclerosis
- 2013
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Ingår i: Nature Genetics. - : Nature Publishing Group. - 1061-4036 .- 1546-1718. ; 45:6, s. 697-
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Tidskriftsartikel (refereegranskat)abstract
- To identify susceptibility genes for amyotrophic lateral sclerosis (ALS), we conducted a genome-wide association study (GWAS) in 506 individuals with sporadic ALS and 1,859 controls of Han Chinese ancestry. Ninety top SNPs suggested by the current GWAS and 6 SNPs identified by previous GWAS were analyzed in an independent cohort of 706 individuals with ALS and 1,777 controls of Han Chinese ancestry. We discovered two new susceptibility loci for ALS at 1q32 (CAMK1G, rs6703183, P-combined = 2.92 x 10(-8), odds ratio (OR) = 1.31) and 22p11 (CABIN1 and SUSD2, rs8141797, P-combined = 2.35 x 10(-9), OR = 1.52). These two loci explain 12.48% of the overall variance in disease risk in the Han Chinese population. We found no association evidence for the previously reported loci in the Han Chinese population, suggesting genetic heterogeneity of disease susceptibility for ALS between ancestry groups. Our study identifies two new susceptibility loci and suggests new pathogenic mechanisms of ALS.
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| 3. |
- Jia, Pan, et al.
(författare)
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The combination of 2d layered graphene oxide and 3d porous cellulose heterogeneous membranes for nanofluidic osmotic power generation
- 2021
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Ingår i: Molecules. - : MDPI AG. - 1420-3049 .- 1420-3049 .- 1431-5157. ; 26:17
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Tidskriftsartikel (refereegranskat)abstract
- Salinity gradient energy, as a type of blue energy, is a promising sustainable energy source. Its energy conversion efficiency is significantly determined by the selective membranes. Recently, nanofluidic membrane made by two-dimensional (2D) nanomaterials (e.g., graphene) with densely packed nanochannels has been considered as a high-efficient membrane in the osmotic power generation research field. Herein, the graphene oxide-cellulose acetate (GO–CA) heterogeneous membrane was assembled by combining a porous CA membrane and a layered GO membrane; the combination of 2D nanochannels and 3D porous structures make it show high surface-charge-governed property and excellent ion transport stability, resulting in an efficient osmotic power harvesting. A power density of about 0.13 W/m2 is achieved for the sea–river mimicking system and up to 0.55 W/m2 at a 500-fold salinity gradient. With different functions, the CA and GO membranes served as ion storage layer and ion selection layer, respectively. The GO–CA heterogeneous membrane open a promising avenue for fabrication of porous and layered platform for wide potential applications, such as sustainable power generation, water purification, and seawater desalination.
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| 4. |
- Xiao, Tian, et al.
(författare)
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Effect of natural convection on charging of phase change materials in graded metal foam : Pore-scale simulation
- 2023
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Ingår i: International Communications in Heat and Mass Transfer. - 0735-1933. ; 149
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Tidskriftsartikel (refereegranskat)abstract
- Open-cell metal foams (MFs) possess exceptional thermal conductivity and are often utilized to enhance the phase transition efficiency of phase change materials (PCMs) that have insufficient thermal conductivity. The movement of energy within MFs during phase transition is influenced by natural convection and heat conduction. In this study, pore-scale numerical simulation (PNS) was employed to study the effect of natural convection on the phase transition of graded MFs (positive gradient foam-PGF, homogenous foam-HF, and negative gradient foam-NGF). Simplified tetrakaidecahedron cells were used for PNS to capture the primary geometric features of MFs. Results have shown that natural convection significantly influences the phase transition process of PCMs. Compared to pure thermal conductivity, the full melting times (FMTs) of PCMs in PGF, HF, and NGF were reduced by 85.2% - 88.3% when natural convection was considered. Furthermore, the integrated average temperature response rates of PCMs in PGF, HF, and NGF, considering natural convection, were improved by 378.4%, 301.9%, and 342.5%, respectively. The presence of natural convection resulted in a gradient in the phase interface distribution and temperature field. The PNS method proved useful in illustrating the influence of metallic ligaments on phase interface and temperature distributions.
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