| 1. |
- Rabionet, Mariona, et al.
(författare)
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Epidermal 1-O-acylceramides appear with the establishment of the water permeability barrier in mice and are produced by maturating keratinocytes
- 2022
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Ingår i: Lipids. - : Wiley. - 0024-4201 .- 1558-9307. ; 57:3, s. 183-195
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Tidskriftsartikel (refereegranskat)abstract
- 1-O-Acylceramides (1-OACs) have a fatty acid esterified to the 1-hydroxyl of the sphingosine head group of the ceramide, and recently we identified these lipids as natural components of human and mouse epidermis. Here we show epidermal 1-OACs arise shortly before birth during the establishment of the water permeability barrier in mice. Fractionation of human epidermis indicates 1-OACs concentrate in the stratum corneum. During in vitro maturation into reconstructed human epidermis, human keratinocytes dramatically increase 1-OAC levels indicating they are one source of epidermal 1-OACs. In search of potential enzymes responsible for 1-OAC synthesis in vivo, we analyzed mutant mice with deficiencies of ceramide synthases (Cers2, Cers3, or Cers4), diacylglycerol acyltransferases (Dgat1 or Dgat2), elongase of very long fatty acids 3 (Elovl3), lecithin cholesterol acyltransferase (Lcat), stearoyl-CoA desaturase 1 (Scd1), or acidic ceramidase (Asah1). Overall levels of 1-OACs did not decrease in any mouse model. In Cers3 and Dgat2-deficient epidermis they even increased in correlation with deficient skin barrier function. Dagt2 deficiency reshapes 1-OAC synthesis with an increase in 1-OACs with N-linked non-hydroxylated fatty acids and a 60% decrease compared to control in levels of 1-OACs with N-linked hydroxylated palmitate. As none of the single enzyme deficiencies we examined resulted in a lack of 1-OACs, we conclude that either there is functional redundancy in forming 1-OAC and more than one enzyme is involved, and/or an unknown acyltransferase of the epidermis performs the final step of 1-OAC synthesis, the implications of which are discussed.
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| 2. |
- Wintersberger, Philipp, et al.
(författare)
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BikeSimWS : Workshop on Simulators, Scenarios, and Test Standard for Bicycle Research
- 2023
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Ingår i: Proceedings of MUM 2023 - The 22nd International Conference on Mobile and Ubiquitous Multimedia. - : Association for Computing Machinery (ACM). ; , s. 546-549
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Konferensbidrag (refereegranskat)abstract
- Research on cyclists"safety and comfort is a growing topic. Existing works address support systems with novel interaction concepts such as augmented reality but also the design and evaluation of high-fidelity bicycle simulators. Since the field is still in its exploratory phase, there have been few attempts to systematically provide guidance for conducting experiments. For example, there is no consensus on the choice of representative driving scenarios, the proper choice of different bicycle simulators, and measurement standards to systematically compare the results of different studies. With this workshop, we want the community to gather and discuss a roadmap for the future of HCI bicycle research so that these issues can be overcome.
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