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Search: WFRF:(Ederth J)

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  • Kish, L.B., et al. (author)
  • In situ electrical transport measurements and self-organization in gold nanoparticle films during and after deposition
  • 2001
  • In: Surface & Coatings Technology. - 0257-8972 .- 1879-3347. ; 142-144, s. 1088-1093
  • Journal article (peer-reviewed)abstract
    • In the first part of this paper, a new method is shown which is able to predict the particle size of conductive nanoparticle films in situ, during deposition. The method is used for the measurement of the time-derivative of conductance fluctuations during deposition. The second part of the paper deals with in situ conductance measurements on gold films during deposition. During the measurement, various electrical fields have been applied in order to study the possible influence of the field magnitude on the film formation. The observed effects have been interpreted by the biased percolation model. A possible technological application of the observed phenomenon is the fabrication of nanoparticle films with controlled disorder, e.g. in chemical sensors. © 2001 Elsevier Science B.V. All rights reserved.
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  • Kish, L.B., Otten, F., Vandamme, L.K.J., Vajtai, R., Granqvist, C.G., Marlow, B., Kruis, E., Fissan, H., Ederth, J., Chaoguang, P. (author)
  • Noise Measurements and Fluctuation Analysis in Nanoparticle Films
  • 2001
  • In: Advanced Research Workshop on Semiconductor Nanostructures, 2001, New Zealand. Paper presented by L.B. Kish, as an invited talk, at the Advanced Research Workshop on Semiconductor Nanostructures, 5-9 February, 2001, Qeenstown, New Zealand..
  • Conference paper (other academic/artistic)
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  • Schmitt, F-J, et al. (author)
  • Direct force measurements on bulk polystyrene using the bimorph surface forces apparatus
  • 1999
  • In: Journal of Adhesion Science and Technology. - 0169-4243 .- 1568-5616. ; 13, s. 79-96
  • Journal article (peer-reviewed)abstract
    • Interfacial properties of bulk polymer samples have been characterized by direct measurements of long-range and adhesion forces using a non-interferometric bimorph surface forces apparatus. For this purpose we developed a method where bulk polystyrene was melted and formed into drop-shaped surfaces with a cylindrical shaft. In the case of very smooth polystyrene samples the adhesion in air and water was very strong and when brought into contact a cold welding of the polymers sometimes took place. Upon separation, it was found that a cohesive failure occurred and the surfaces were locally damaged. In the case of less flat polystyrene surfaces, the adhesion increased with the applied (external) load to a saturation value. We attribute this to a local flattening of nanometer sized protrusions. The long-range interaction in water is dominated by a weak electrostatic double-layer force. At separations below about 10 nm, an attractive force component predominates the interaction. The adhesion in aqueous solutions decreased dramatically in the presence of a nonionic surfactant, n-octyl b-D-glucopyranoside. The surfactant adsorbs onto hydrophobic surfaces, such as the polystyrene surface used here, and close to the critical micellar concentration it forms an oriented monolayer where the polar sugar units are directed towards the solution. The range of the short-range repulsion between polystyrene surfaces coated with a monolayer of n-octyl b-D-glucopyranoside is about 2-3 nm, slightly larger than observed previously for such surfactant layers adsorbed onto very flat hydrophobized mica surfaces. Further, a considerably stronger attractive force is observed upon separation of the surfactant coated surfaces in case of polystyrene compared to when hydrophobized mica is used. The influence of the substrate surface on the interaction can be rationalized by their differences in surface roughness.
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  • Ederth, J, et al. (author)
  • Ag-Mn nanoparticles: Three-dimensional finite size effect of the spin glass state
  • 1999
  • In: JOURNAL OF APPLIED PHYSICS. - : AMER INST PHYSICS. - 0021-8979. ; 86:11, s. 6571-6575
  • Journal article (peer-reviewed)abstract
    • Ag-Mn nanoparticles were prepared by advanced gas evaporation. Their composition corresponded to Ag0.89Mn0.11, and the particle size distribution was log normal. The particles demonstrated an unambiguous spin glass behavior with the temperature depen
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  • Ederth, Thomas, et al. (author)
  • Anomalous settlement behavior of Ulva linza zoospores on cationic oligopeptide surfaces
  • 2008
  • In: Biofouling (Print). - : Informa UK Limited. - 0892-7014 .- 1029-2454. ; 24:4, s. 303-312
  • Journal article (peer-reviewed)abstract
    • Identification of settlement cues for marine fouling organisms opens up new strategies and methods for biofouling prevention, and enables the development of more effective antifouling materials. To this end, the settlement behaviour of zoospores of the green alga Ulva linza onto cationic oligopeptide self-assembled monolayers (SAMs) has been investigated. The spores interact strongly with lysine- and arginine-rich SAMs, and their settlement appears to be stimulated by these surfaces. Of particular interest is an arginine-rich oligopeptide, which is effective in attracting spores to the surface, but in a way which leaves a large fraction of the settled spores attached to the surface in an anomalous fashion. These 'pseudo-settled' spores are relatively easily detached from the surface and do not undergo the full range of cellular responses associated with normal commitment to settlement. This is a hitherto undocumented mode of settlement, and surface dilution of the arginine-rich peptide with a neutral triglycine peptide demonstrates that both normal and anomalous settlement is proportional to the surface density of the arginine-rich peptide. The settlement experiments are complemented with physical studies of the oligopeptide SAMs, before and after extended immersion in artificial seawater, using infrared spectroscopy, null ellipsometry and contact angle measurements.
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  • Ederth, Thomas, et al. (author)
  • Interactions of Zoospores of Ulva linza with Arginine-Rich Oligopeptide Monolayers
  • 2009
  • In: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 25:16, s. 9375-9383
  • Journal article (peer-reviewed)abstract
    • We recently reported oil the strong interactions of zoospores of the green alga, Ulva linza with all arginine-rich oligopeptide self-assembled monolayer (SAM) [Biofouling 2008, 24, 303-312], where the arginine-rich peptide induced not only high spore settlement, but also a form of abnormal settlement, or "pseudo-settlement", whereby it proportion of spores do not go through the normal process of surface exploration, adhesive exocytosis, and loss of flagella. Further. it was demonstrated that both the total number of settled spores and the fraction of pseudosettled spores were related to the surface density of the arginine-rich peptide. Here we present a further investigation of the interactions of zoospores of ulva with a set of oligomeric, de nom designed, arginine-rich peptides, specifically aimed to test the effect of peptide primary structure on the interaction. Via variations in the peptide length and by permutations in the amino acid sequences, we gain further insight into the spore-surface interactions. The interpretation of the biological assays is supported by physicochemical characterization of the SAMs using infrared spectroscopy, ellipsometry, and contact angle measurement. Results confirm the importance of arginine residues for the anomalous pseudosettlement, and we found that settlement is modulated by variations in both the total length and peptide primary structure. To elucidate the Causes of the anomalous settlement and the possible relation to peptide-membrane interactions, we also compared the settlement of the "naked" zoospores of Ulva(which present it lipoprotein membrane to the exterior without a discrete polysaccharide cell wall), with the settlement of diatoms (unicellular algae that are surrounded by it silica cell wall), onto the peptide SAMs. Cationic SAMs do not notably affect settlement (attachment), adhesion strength, or viability of diatom cells, Suggesting that the effect of the peptides on zoospores of Ulva is mediated via specific peptide-membrane interactions.
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  • Ederth, Thomas, et al. (author)
  • Resistance of Galactoside-Terminated Alkanethiol Self-Assembled Monolayers to Marine Fouling Organisms
  • 2011
  • In: ACS Applied Materials and Interfaces. - : American Chemical Society. - 1944-8244 .- 1944-8252. ; 3:10, s. 3890-3901
  • Journal article (peer-reviewed)abstract
    • Self-assembled monolayers (SAMs) of galactoside-terminated alkanethiols have protein-resistance properties which can be tuned via the degree of methylation [Langmuir 2005, 21, 2971-2980]. Specifically, a partially methylated compound was more resistant to nonspecific protein adsorption than the hydroxylated or fully methylated counterparts. We investigate whether this also holds true for resistance to the attachment and adhesion of a range of marine species, in order to clarify to what extent resistance to protein adsorption correlates with the more complex adhesion of fouling organisms. The partially methylated galactoside-terminated SAM was further compared to a mixed monolayer of omega-substituted methyl- and hydroxyl-terminated alkanethiols with wetting properties and surface ratio of hydroxyl to methyl groups matching that of the galactoside. The settlement (initial attachment) and adhesion strength of four model marine fouling organisms were investigated, representing both micro- and macrofoulers; two bacteria (Cobetia marina and Marinobacter hydrocarbonoclasticus), barnacle cypris larvae (Balanus amphitrite), and algal zoospores (Ulva linza). The minimum in protein adsorption onto the partially methylated galactoside surface was partly reproduced in the marine fouling assays, providing some support for a relationship between protein resistance and adhesion of marine fouling organisms. The mixed alkanethiol SAM, which was matched in wettability to the partially methylated galactoside SAM, consistently showed higher settlement (initial attachment) of test organisms than the galactoside, implying that both wettability and surface chemistry are insufficient to explain differences in fouling resistance. We suggest that differences in the structure of interfacial water may explain the variation in adhesion to these SAMs.
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  • Halvardson, Jonatan, et al. (author)
  • Mutations in HECW2 are associated with intellectual disability and epilepsy
  • 2016
  • In: Journal of Medical Genetics. - : BMJ. - 0022-2593 .- 1468-6244. ; 53:10, s. 697-704
  • Journal article (peer-reviewed)abstract
    • BACKGROUND: De novo mutations are a frequent cause of disorders related to brain development. We report the results of screening patients diagnosed with both epilepsy and intellectual disability (ID) using exome sequencing to identify known and new causative de novo mutations relevant to these conditions.METHODS: Exome sequencing was performed on 39 patient-parent trios to identify de novo mutations. Clinical significance of de novo mutations in genes was determined using the American College of Medical Genetics and Genomics standard guidelines for interpretation of coding variants. Variants in genes of unknown clinical significance were further analysed in the context of previous trio sequencing efforts in neurodevelopmental disorders.RESULTS: In 39 patient-parent trios we identified 29 de novo mutations in coding sequence. Analysis of de novo and inherited variants yielded a molecular diagnosis in 11 families (28.2%). In combination with previously published exome sequencing results in neurodevelopmental disorders, our analysis implicates HECW2 as a novel candidate gene in ID and epilepsy.CONCLUSIONS: Our results support the use of exome sequencing as a diagnostic approach for ID and epilepsy, and confirm previous results regarding the importance of de novo mutations in this patient group. The results also highlight the utility of network analysis and comparison to previous large-scale studies as strategies to prioritise candidate genes for further studies. This study adds knowledge to the increasingly growing list of causative and candidate genes in ID and epilepsy and highlights HECW2 as a new candidate gene for neurodevelopmental disorders.
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  • Kish, LB, et al. (author)
  • Noise measurements and fluctuation analysis in nanoparticle films
  • 2001
  • In: PHYSICA E. - : ELSEVIER SCIENCE BV. - 1386-9477. ; 11:2-3, s. 131-136
  • Journal article (peer-reviewed)abstract
    • This work reports two different ways of study providing potentially important information about nanoparticle films. The first study is about conductance noise in PbS nanoparticle films. Monocrystalline and single-sized PbS nanoparticles are synthesized vi
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  • Klenkar, Goran, et al. (author)
  • Addressable adsorption of lipid vesicles and subsequent protein interaction studies
  • 2008
  • In: Biointerphases. - : American Vacuum Society. - 1559-4106 .- 1934-8630. ; 3:2, s. 29-37
  • Journal article (peer-reviewed)abstract
    • We demonstrate a convenient chip platform for the addressable immobilization of protein-loaded vesicles on a microarray for parallelized, high-throughput analysis of lipid-protein systems. Self-sorting of the vesicles on the microarray was achieved through DNA bar coding of the vesicles and their hybridization to complementary strands, which are preimmobilized in defined array positions on the chip. Imaging surface plasmon resonance in ellipsometric mode was used to monitor vesicle immobilization, protein tethering, protein-protein interactions, and chip regeneration. The immobilization strategy proved highly specific and stable and presents a mild method for the anchoring of vesicles to predefined areas of a surface, while unspecific adsorption to both noncomplementary regions and background areas is nonexistent or, alternatively, undetectable. Furthermore, histidine-tagged receptors have been stably and functionally immobilized via bis-nitrilotriacetic acid chelators already present in the vesicle membranes. It was discovered though that online loading of proteins to immobilized vesicles leads to cross contamination of previously loaded vesicles and that it was necessary to load the vesicles offline in order to obtain pure protein populations on the vesicles. We have used this cross-binding effect to our benefit by coimmobilizing two receptor subunits in different ratios on the vesicle surface and successfully demonstrated ternary complex formation with their ligand. This approach is suitable for mechanistic studies of complex multicomponent analyses involving membrane-bound systems.
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  • Tilbury, Maura A., et al. (author)
  • The expression and characterization of recombinant cp19k barnacle cement protein from Pollicipes pollicipes
  • 2019
  • In: Philosophical Transactions of the Royal Society of London. Biological Sciences. - : ROYAL SOC. - 0962-8436 .- 1471-2970. ; 374:1784
  • Journal article (peer-reviewed)abstract
    • Adhesive proteins of barnacle cement have potential as environmentally friendly adhesives owing to their ability to adhere to various substrates in aqueous environments. By understanding the taxonomic breath of barnacles with different lifestyles, we may uncover commonalities in adhesives produced by these specialized organisms. The 19 kDa cement protein (cp19k) of the stalked barnacle Pollicipes pollicipes was expressed in Escherichia coli BL21 to investigate its adhesive properties. Initial expression of hexahistidine-tagged protein (rPpolcp19k-his) yielded low levels of insoluble protein. Co-overproduction of E. coli molecular chaperones GroEL-GroES and trigger factor (TF) increased soluble protein yields, although TF co-purified with the target protein (TF-rPpolcp19k-his). Surface coat analysis revealed high levels of adsorption of the TF-rPpolcp19k-his complex and of purified E. coli TF on both hydrophobic and hydrophilic surfaces, while low levels of adsorption were observed for rPpolcp19k-his. Tag-free rPpolcp19k protein also exhibited low adsorption compared to fibrinogen and Cell-Tak controls on hydrophobic, neutral hydrophilic and charged self-assembled monolayers under surface plasmon resonance assay conditions designed to mimic the barnacle cement gland or seawater. Because rPpolcp19k protein displays low adhesive capability, this protein is suggested to confer the ability to self-assemble into a plaque within the barnacle cement complex. This article is part of the theme issue Transdisciplinary approaches to the study of adhesion and adhesives in biological systems.
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  • Wibisono, Yusuf, et al. (author)
  • Hydrogel-coated feed spacers in two-phase flow cleaning in spiral wound membrane elements: A novel platform for eco-friendly biofouling mitigation
  • 2015
  • In: Water Research. - : Elsevier / IWA Publishing. - 0043-1354 .- 1879-2448. ; 71, s. 171-186
  • Journal article (peer-reviewed)abstract
    • Biofouling is still a major challenge in the application of nanofiltration and reverse osmosis membranes. Here we present a platform approach for environmentally friendly biofouling control using a combination of a hydrogel-coated feed spacer and two-phase flow cleaning. Neutral (polyHEMA-co-PEG(10)MA), cationic (polyDMAEMA) and anionic (polySPMA) hydrogels have been successfully grafted onto polypropylene (PP) feed spacers via plasma-mediated UV-polymerization. These coatings maintained their chemical stability after 7 days incubation in neutral (pH 7), acidic (pH 5) and basic (pH 9) environments. Anti-biofouling properties of these coatings were evaluated by Escherichia coli attachment assay and nanofiltration experiments at a TMP of 600 kPag using tap water with additional nutrients as feed and by using optical coherence tomography. Especially the anionic polySPMA-coated PP feed spacer shows reduced attachment of E. coli and biofouling in the spacer-filled narrow channels resulting in delayed biofilm growth. Employing this highly hydrophilic coating during removal of biofouling by two-phase flow cleaning also showed enhanced cleaning efficiency, feed channel pressure drop and flux recoveries. The strong hydrophilic nature and the presence of negative charge on polySPMA are most probably responsible for the improved antifouling behavior. A combination of polySPMA-coated PP feed spacers and two-phase flow cleaning therefore is promising and an environmentally friendly approach to control biofouling in NF/RO systems employing spiral-wound membrane modules.
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