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- Ekblom, Robert, Docent, 1976-, et al.
(författare)
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Development of transcriptome genetic markers for the great snipe (Gallinago media)
- 2017
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Ingår i: Conservation Genetics Resources. - : SPRINGER. - 1877-7252 .- 1877-7260. ; 9:4, s. 643-645
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Tidskriftsartikel (refereegranskat)abstract
- We sequenced the transcriptomes of 14 great snipe (Gallinago media) males from the Gavalia study population (central Norway) using Roche 454 technology. The assembled transcriptome sequences (RNA-Seq) was used to identify 140 microsatellite repeat sequences with sufficient flanking sequence information for primer design. In addition several 1000 single nucleotide polymorphisms in the transcriptome were identified, and a small subset of these were verified by independent genotyping.
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| 10. |
- Ekblom, Robert, Docent, 1976-
(författare)
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Dubbelbeckasinens spel
- 2007
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Ingår i: Fauna och flora. - 0014-8903. ; 102, s. 2-4
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 11. |
- Ekblom, Robert, Docent, 1976-
(författare)
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Dubbelbeckasinens spel
- 2006
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Ingår i: Vår Fågelvärld. - 0042-2649. ; :3, s. 20-21
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 12. |
- Ekblom, Robert, Docent, 1976-, et al.
(författare)
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Genome sequencing and conservation genomics in the Scandinavian wolverine population
- 2018
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Ingår i: Conservation Biology. - : WILEY. - 0888-8892 .- 1523-1739. ; 32:6, s. 1301-1312
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Tidskriftsartikel (refereegranskat)abstract
- Genetic approaches have proved valuable to the study and conservation of endangered populations, especially for monitoring programs, and there is potential for further developments in this direction by extending analyses to the genomic level. We assembled the genome of the wolverine (Gulo gulo), a mustelid that in Scandinavia has recently recovered from a significant population decline, and obtained a 2.42 Gb draft sequence representing >85% of the genome and including >21,000 protein-coding genes. We then performed whole-genome resequencing of 10 Scandinavian wolverines for population genomic and demographic analyses. Genetic diversity was among the lowest detected in a red-listed population (mean genome-wide nucleotide diversity of 0.05%). Results of the demographic analyses indicated a long-term decline of the effective population size (N-e) from 10,000 well before the last glaciation to N-e appeared even lower. The genome-wide F-IS level was 0.089 (possibly signaling inbreeding), but this effect was not observed when analyzing a set of highly variable SNP markers, illustrating that such markers can give a biased picture of the overall character of genetic diversity. We found significant population structure, which has implications for population connectivity and conservation. We used an integrated microfluidic circuit chip technology to develop an SNP-array consisting of 96 highly informative markers that, together with a multiplex pre-amplification step, was successfully applied to low-quality DNA from scat samples. Our findings will inform management, conservation, and genetic monitoring of wolverines and serve as a genomic roadmap that can be applied to other endangered species. The approach used here can be generally utilized in other systems, but we acknowledge the trade-off between investing in genomic resources and direct conservation actions.
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- Ekblom, Robert, Docent, 1976-
(författare)
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På vinterinventering
- 2006
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Ingår i: Vår Fågelvärld. - 0042-2649. ; :8, s. 32-34
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 23. |
- Ekblom, Robert, Docent, 1976-, et al.
(författare)
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Sample identification and pedigree reconstruction in Wolverine (Gulo gulo) using SNP genotyping of non-invasive samples
- 2021
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Ingår i: Conservation Genetics Resources. - : Springer Nature. - 1877-7252 .- 1877-7260. ; 13:3, s. 261-274
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Tidskriftsartikel (refereegranskat)abstract
- For conservation genetic studies using non-invasively collected samples, genome-wide data may be hard to acquire. Until now, such studies have instead mostly relied on analyses of traditional genetic markers such as microsatellites (SSRs). Recently, high throughput genotyping of single nucleotide polymorphisms (SNPs) has become available, expanding the use of genomic methods to include non-model species of conservation concern. We have developed a 96-marker SNP array for use in applied conservation monitoring of the Scandinavian wolverine (Gulo gulo) population. By genotyping more than a thousand non-invasively collected samples, we were able to obtain precise estimates of different types of genotyping errors and sample dropout rates. The SNP panel significantly outperforms the SSR markers (and DBY intron markers for sexing) both in terms of precision in genotyping, sex assignment and individual identification, as well as in the proportion of samples successfully genotyped. Furthermore, SNP genotyping offers a simplified laboratory and analysis pipeline with fewer samples needed to be repeatedly genotyped in order to obtain reliable consensus data. In addition, we utilised a unique opportunity to successfully demonstrate the application of SNP genotype data for reconstructing pedigrees in wild populations, by validating the method with samples from wild individuals with known relatedness. By offering a simplified workflow with improved performance, we anticipate this methodology will facilitate the use of non-invasive samples to improve genetic management of many different types of populations that have previously been challenging to survey.
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| 24. |
- Ekblom, Robert, Docent, 1976-
(författare)
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Smörbollsflugornas fantastiska värld
- 2007
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Ingår i: Fauna och flora. - 0014-8903. ; 201, s. 20-22
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 26. |
- Gunnarsson, Karl, et al.
(författare)
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Review of the diet specialisation of the Blue-cheeked bee-eater (Merops persicus)
- 2019
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Ingår i: Journal of Ornithology. - : SPRINGER HEIDELBERG. - 2193-7192 .- 2193-7206. ; 160:1, s. 275-279
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Forskningsöversikt (refereegranskat)abstract
- Ecological specialisation, e.g. in terms of prey preferences, can have important implications for population biology. Bee-eaters are considered diet specialists; they hunt aerial insects, particularly hymenopterans. However, the Blue-cheeked bee-eater (Merops persicus) has been considered a special case, as it preferentially feeds upon dragonflies (Odonata). Here we review current literature on the diet of the Blue-cheeked bee-eater, and conclude that this species is not as restricted to dragonflies as previously suggested. Instead, populations in different parts of the breeding and wintering ranges seem to specialise on various types of insects (such as wasps, bees, dragonflies, butterflies and mosquitoes).
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| 27. |
- Lindström, Åke, et al.
(författare)
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Extreme altitude changes between night and day during marathon flights of great snipes
- 2021
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Ingår i: Current Biology. - : Elsevier BV. - 0960-9822 .- 1879-0445. ; 31:15, s. 3433-3439
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Tidskriftsartikel (refereegranskat)abstract
- Summary Several factors affect the flight altitude of migratory birds, such as topography, ambient temperature, wind conditions, air humidity, predation avoidance, landmark orientation, and avoiding over-heating from direct sunlight.1, 2, 3, 4, 5, 6 Recent tracking of migratory birds over long distances has shown that migrants change flight altitude more commonly and dramatically than previously thought.4, 5, 6, 7, 8 The reasons behind these altitude changes are not well understood. In their seasonal migrations between Sweden and sub-Saharan Africa, great snipes Gallinago media make non-stop flights of 4,000–7,000 km, lasting 60–90 h.9,10 Activity and air pressure data from multisensor dataloggers showed that great snipes repeatedly changed altitudes around dawn and dusk, between average cruising heights about 2,000 m (above sea level) at night and around 4,000 m during daytime. Frequency and autocorrelation analyses corroborated a conspicuous diel cycle in flight altitude. Most birds regularly flew at 6,000 m and one bird reached 8,700 m, possibly the highest altitude ever recorded for an identified migrating bird. The diel altitude changes took place independently of climate zone, topography, and habitat overflown. Ambient temperature, wind condition, and humidity have no important diel variation at the high altitudes chosen by great snipes. Instead, improved view for orientation by landmarks, predator avoidance, and not least, seeking cold altitudes at day to counteract heating from direct sunlight are the most plausible explanations for the diel altitude cycle. Together with similar recent findings for a small songbird,6 the great snipes’ altitudinal performance sheds new light on the complexity and challenges of migratory flights.
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