| 1. |
- Pierzynowski, Stefan, et al.
(författare)
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Exogenous pancreatic-like enzymes are recovered in the gut and improve growth of exocrine pancreatic insufficient pigs
- 2012
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Ingår i: Journal of Animal Science. - : Oxford University Press (OUP). - 1525-3163 .- 0021-8812. ; 90, s. 324-326
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Tidskriftsartikel (refereegranskat)abstract
- The exocrine pancreatic insufficient (EPI) pigs grow less due to different disturbances in feed digestion, absorption, and retention. Use of pancreatic-like enzymes of microbial origin in pigs may improve feed use and performance in slow-growing pigs. The aim was to study gut recovery and effectiveness of pancreatic-like enzymes of microbial origin supplementation on pig performance. Six male pigs 10 to 12 kg BW underwent pancreatic duct ligation surgery to induce total exocrine pancreatic insufficiency (EPI). Three cannulas to access the gastrointestinal tract content were installed in stomach, duodenum, and ileum in EPI pigs and in 3 control (healthy) pigs. One month after surgery, enzymes were given before feeding and digesta samples were collected for analyses. The BW of EPI pigs did not increase during 1 mo following surgery (11.7 vs. 11.6 kg BW); however, BW increased after 1 wk of enzyme supplementation (12.1 kg BW). Coefficient of fat and N absorption increased (P < 0.05) in EPI pigs after enzyme supplementation. Activity of amylase, lipase, and protease in chyme samples of EPI pigs was very low compared to controls. In EPI pigs after enzyme supplementation, amylase activity increased from 5.32 to 72.9 units/mL but remained lower than that of healthy pigs (162.7 units/mL). Lipase activity increased from 79.1 to 421.6 units/mL, which was similar to that of controls (507.3 units/mL). Proteolytic activity increased from 7.8 to 69.7 units/mL but still did not reach control pigs (164.3 units/mL). In conclusion, exogenous microbial enzymes mimic endogenous pancreatic enzymes being recovered along the lumen of the gastrointestinal tract. These enzymes might be a useful tool to stimulate growth of slower-growing pigs after the weaning period.
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| 2. |
- Prykhodko, O., et al.
(författare)
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Early treatment with pancreatic-like microbial-derived enzymes during the preweaning period promotes growth in growing–finishing pigs
- 2016
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Ingår i: Journal of Animal Science. - : Oxford University Press (OUP). - 0021-8812 .- 1525-3163. ; 94:7, s. 150-152
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Tidskriftsartikel (refereegranskat)abstract
- Treatment with pancreatic or pancreatic-like microbial enzymes during the suckling period has positive effects on the gut, resulting in a better fat absorption and improved barrier function in pigs. The present study investigated the effects of pancreaticlike enzyme treatment during the pre- or postweaning period on pig growth and nitrogen utilization. Suckling 7- to 14-d-old pigs were gavage fed with a microbial-derived enzyme preparation (amylase, protease, and lipase) in a split-litter mode (13 litters) once (n = 29), twice (n = 35), or untreated (controls; n = 66). All pigs were then raised under the same standard production conditions and received the same diets, and their BW and feed intake were monitored until slaughter. Another set of 35-d-old pigs, on the day of weaning, were placed in metabolic cages and fed a diet with or without enzyme supplementation for 2 wk (n = 4/group). Feed consumption was measured daily, and at the end of each week, BW was recorded and urine and feces were collected during 72 h for nitrogen analysis. Dietary enzyme supplementation after weaning in selected doses had no major effect on feed consumption, nitrogen retention, or growth compared with the control. In contrast, enzyme treatment during the suckling period gave improved growth, with increased BW at 6 mo of age, 105 ± 16 vs. 97 ± 15 kg for the controls (P < 0.05), and those pigs reached slaughter weight earlier than untreated controls. In addition, the feed conversion ratio was significantly decreased, thus lowering nitrogen excretion per kilogram BW gain in the enzymetreated group compared with the untreated controls (3.0 vs. 2.7, respectively). We conclude that enzyme supplementation during the suckling period was beneficial for both pig performance and environment protection, probably due to an induced improved maturation of the gut having a long-term impact on feed utilization and growth after weaning.
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| 3. |
- Slupecka, M., et al.
(författare)
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Stimulating effect of pancreatic-like enzymes on the development of the gastrointestinal tract in piglets
- 2012
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Ingår i: Journal of Animal Science. - 1525-3163. ; 90, s. 311-314
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Tidskriftsartikel (refereegranskat)abstract
- Use of nutritional components from the milk and eventually from the solid feed relates to the growth and development of gastrointestinal tract (GIT). We studied the effect of pancreatic-like enzymes [porcine pancreatic enzymes (Creon) or microbial-derived amylase, protease, and lipase] on GIT morphology and lipid absorption in suckling piglets. Both enzyme preparations, in low or high dose, were fed via a stomach tube twice a day for 7 d starting at 8 d of age and controls received vehicle, n = 6. The day after treatments ended, lipid absorption was tested after which pigs were euthanized and GIT was examined. Enzyme cocktails, irrespective of their origin, increased (P < 0.001) triglyceride level in blood. Enzyme preparation affected (P < 0.001) small intestinal mucosal thickness, villi length, and crypt depth and (P < 0.01) mitotic division of enterocytes. In addition, the external administration of pancreatic enzymes stimulated pancreatic growth as observed by increased (P < 0.05) mitotic division of pancreatic cells. The study revealed that pancreatic or pancreatic-like enzymes of microbial origin administrated in the early postperinatal period enhance GIT development and may be used to better prepare the GIT of piglets for milk use and weaning.
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