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- Mazzuca, Silvia, et al.
(författare)
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Establishing research strategies, methodologies and technologies to link genomics and proteomics to seagrass productivity, community metabolism, and ecosystem carbon fluxes
- 2013
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Ingår i: Frontiers in Plant Science. - : Frontiers Media SA. - 1664-462X. ; 4
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Tidskriftsartikel (refereegranskat)abstract
- A complete understanding of the mechanistic basis of marine ecosystem functioning is only possible through integrative and interdisciplinary research. This enables the prediction of change and possibly the mitigation of the consequences of anthropogenic impacts. One major aim of the European Cooperation in Science and Technology (COST) Action ES0609 Seagrasses productivity. From genes to ecosystem management, is the calibration and synthesis of various methods and the development of innovative techniques and protocols for studying seagrass ecosystems. During 10 days, 20 researchers representing a range of disciplines (molecular biology, physiology, botany, ecology, oceanography, and underwater acoustics) gathered at The Station de Recherches Sous-marines et Oceanographiques (STARESO, Corsica) to study together the nearby Posidonia oceanica meadow. STARESO is located in an oligotrophic area classified as pristine site where environmental disturbances caused by anthropogenic pressure are exceptionally low. The healthy P. oceanica meadow, which grows in front of the research station, colonizes the sea bottom from the surface to 37 m depth. During the study, genomic and proteomic approaches were integrated with ecophysiological and physical approaches with the aim of understanding changes in seagrass productivity and metabolism at different depths and along daily cycles. In this paper we report details on the approaches utilized and we forecast the potential of the data that will come from this synergistic approach not only for P. oceanica but for seagrasses in general.
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| 2. |
- Pagula, Felisberto P., 1956-
(författare)
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Possibilities to Utilize Species from the Mozambican Forest Biomass for the Production of Essential Oils : Study of Eucalyptus and Pinus Species and Application of Citronellal as Raw Material for Flavour Chemistry
- 2004
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis deals partly with the isolation and chemicalanalysis of essential oils from selected Eucalyptus and Pinusspecies grown in Mozambique, and partly with the synthesis ofmore valuable compounds from citronellal isolated fromEucalyptus citriodora Hook oil.More than 60 constituents in the volatile oils wereidentified by gas chromatography and gas chromatography-massspectrometry by comparison with authentic substances andliterature data.The effects of distillation time, age (Eucalyptuscamaldulensis Dehn) and season, for harvesting (E. citriodoraHook) on the yield and the percentage composition of theessential oils were determined. It was found that 60 minuteswas the optimum distillation time required for the isolation ofessential oils from the leaves of Eucalyptus species. The ageof the trees, the harvesting season and the distillation timewere found to be important factors for the composition of theessential oils and for obtaining optimum yields. We showed thatthe yield was highest from the older trees and that the majorvariations in oil composition occurred among the monoterpenehydrocarbons (E. camaldulensis). In general, the highest yieldswere obtained during the warm summer and an increase ofcitronellal content (E. citriodora) was also observed at thatseason.The enantiomeric composition of selected chiral monoterpenehydrocarbons and oxygenated compounds in the essential oil fromE. camaldulensis were briefly investigated. Differences in theratios of (+) to (-) enantiomers of specific compounds werefound in oils from younger and older trees.The synthesis of valuable compounds, hydroxycitronellal andcis/trans-rose oxide, from citronellal were also studied. Thehydroxycitronellal synthesis focused on chemicaltransformations using two approaches. The procedure for thesynthesis of isomeric cis/trans-rose oxide was focused onsynthesizing 3,7-dimethyl-5-octene-1,7-diol and3,7-dimethyl-7-octene-1,6-diol from citronellal by simultaneousphotooxidation and reduction followed by acid catalyzedcyclization.Keywords:Eucalyptus species, Myrtaceae, essential oilcomposition, enantiomeric composition, monoterpene, seasonalvariation, citronellal, photooxidation, acetal, epoxidation,Swern oxidation, hydroxycitronellal, cis/trans-rose oxide.
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