| 1. |
- Fernström, Erik, et al.
(författare)
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Results of preoperative chemoradiotherapy for patients with advanced cancer of the nasal cavity and paranasal sinuses.
- 2017
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Ingår i: Acta oto-laryngologica. - : Informa UK Limited. - 1651-2251 .- 0001-6489. ; 137:12, s. 1292-1300
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Tidskriftsartikel (refereegranskat)abstract
- Curative treatment of nasal cavity and paranasal sinus cancer is challenging due to the proximity to critical anatomical structures. The purpose of this study was to analyze the impact of trimodality therapy with preoperative chemotherapy and reduced-dose radiotherapy followed by organ-preserving surgery for treating patients with nasal cavity and paranasal sinus cancer.This retrospective study included all 156 patients diagnosed with sinonasal cancer in western Sweden between 1986 and 2009. We determined the treatment selection pattern and treatment outcomes for 79 patients treated with preoperative chemoradiotherapy.Squamous cell carcinoma was the most common histology. The five-year overall survival was 54%, and 85% of these patients had T3 or T4 tumors. The five-year cumulative incidence rate of local recurrence was 32%. The five-year overall survival in patients with squamous cell carcinoma and adenocarcinoma was 45% and 76%, respectively. The median preoperative radiation dose was 48Gy. Orbital exenteration was performed in 7% of patients.Preoperative chemoradiotherapy may be beneficial for patients with advanced sinonasal cancer when primary radical surgery is challenging. Survival outcomes were comparable to outcomes reported in the literature despite conservative surgery and relatively low radiation doses in patients with locally advanced tumors.
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| 2. |
- Eimer, Johannes, et al.
(författare)
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Spiroplasma ixodetis Infections in Immunocompetent and Immunosuppressed Patients after Tick Exposure, Sweden
- 2022
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Ingår i: Emerging Infectious Diseases. - : Centers for Disease Control and Prevention (CDC). - 1080-6040 .- 1080-6059. ; 28:8, s. 1681-1685
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Tidskriftsartikel (refereegranskat)abstract
- We report 2 cases of Spiroplasma ixodetis infection in an immunocompetent patient and an immunocompromised patient who had frequent tick exposure. Fever, thrombocytopenia, and increased liver aminotransferase levels raised the suspicion of anaplasmosis, but 16S rRNA PCR and Sanger sequencing yielded a diagnosis of spiroplasmosis. Both patients recovered after doxycycline treatment.
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| 3. |
- Fernström, Anna-Linnea, et al.
(författare)
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Stress in cynomolgus monkeys (Macaca fascicularis) subjected to long-distance transport and simulated transport housing conditions
- 2008
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Ingår i: Stress. - : Informa UK Limited. - 1025-3890 .- 1607-8888. ; 11:6, s. 467-476
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Tidskriftsartikel (refereegranskat)abstract
- The stress associated with transportation of non-human primates used in scientific research is an important but almost unexplored part of laboratory animal husbandry. The procedures and routines concerning transport are not only important for the animals' physical health but also for their mental health as well. The transport stress in cynomolgus monkeys (Macaca fascicularis) was studied in two experiments. In Experiment 1, 25 adult female cynomolgus monkeys were divided into five groups of five animals each that received different diets during the transport phase of the experiment. All animals were transported in conventional single animal transport cages with no visual or tactile contact with conspecifics. The animals were transported by lorry for 24 h at ambient temperatures ranging between 20 degrees C and 35 degrees C. Urine produced before, during and after transport was collected and analysed for cortisol by enzyme-linked immunosorbent assay (ELISA). All monkeys exhibited a significant increase in cortisol excretion per time unit during the transport and on the first day following transport.Although anecdotal reports concerning diet during transport, including the provision of fruits and/or a tranquiliser, was thought likely to influence stress responses, these were not corrobated by the present study. In Experiment 2, behavioural data were collected from 18 cynomolgus macaques before and after transfer from group cages to either single or pair housing, and also before and after a simulated transport, in which the animals were housed in transport cages. The single housed monkeys were confined to single transport cages and the pair housed monkeys were kept in their pairs in double size cages. Both pair housed and singly housed monkeys showed clear behavioural signs of stress soon after their transfer out of their group cages.However, stress-associated behaviours were more prevalent in singly housed animals than in pair housed animals, and these behaviours persisted for a longer time after the simulated transport housing event than in the pair housed monkeys. Our data confirm that the transport of cynomolgus monkeys is stressful and suggest that it would be beneficial for the cynomolgus monkeys to be housed and transported in compatible pairs from the time they leave their group cages at the source country breeding facility until they arrive at their final laboratory destination in the country of use.
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| 4. |
- Fernström, Erik, et al.
(författare)
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Cerebrospinal fluid markers of extracellular matrix remodelling, synaptic plasticity and neuroinflammation before and after cranial radiotherapy
- 2018
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Ingår i: Journal of Internal Medicine. - : Wiley. - 0954-6820. ; 284:2, s. 211-225
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Tidskriftsartikel (refereegranskat)abstract
- Background Advances in the treatment of brain tumours have increased the number of long-term survivors, but at the cost of side effects following cranial radiotherapy ranging from neurocognitive deficits to outright tissue necrosis. At present, there are no tools reflecting the molecular mechanisms underlying such side effects, and thus no means to evaluate interventional effects after cranial radiotherapy. Therefore, fluid biomarkers are of great clinical interest. Objective Cerebrospinal fluid (CSF) levels of proteins involved in inflammatory signalling, synaptic plasticity and extracellular matrix (ECM) integrity were investigated following radiotherapy to the brain. Methods Patients with small-cell lung cancer (SCLC) eligible for prophylactic cranial irradiation (PCI) were asked to participate in the study. PCI was prescribed either as 2 Gy/fraction to a total dose of 30 Gy (limited disease) or 4 Gy/fraction to 20 Gy (extensive disease). CSF was collected by lumbar puncture at baseline, 3 months and 1 year following PCI. Protein concentrations were measured using immunobased assays or mass spectrometry. Results The inflammatory markers IL-15, IL-16 and MCP-1/CCL2 were elevated in CSF 3 months following PCI compared to baseline. The plasticity marker GAP-43 was elevated 3 months following PCI, and the same trend was seen for SNAP-25, but not for SYT1. The investigated ECM proteins, brevican and neurocan, showed a decline following PCI. There was a strong correlation between the progressive decline of soluble APP and brevican levels. Conclusion To our knowledge, this is the first time ECM-related proteins have been shown to be affected by cranial radiotherapy in patients with cancer. These findings may help us to get a better understanding of the mechanisms behind side effects following radiotherapy.
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| 5. |
- Fernström, Erik, et al.
(författare)
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Die Gebirgsexpedition Trantre
- 1979
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Annan publikation (populärvet., debatt m.m.)abstract
- Im Winter 1978 wurde die Gebirgsexpedition „Fjällmarsch TranTre“ von sechs jungen Männern auf Skiern durchgeführt. Er begann am 1. März in Transtrand und ging entlang der schwedischen Bergkette bis nach Treriksröset. Dann reisten sie nach Süden zum Torne Träsk bahnhof, den sie am 27. April erreichten.Sie wanderten 50 Tage lang und legten eine Strecke von 1500 km zurück. Die jungen Männer dokumentierten ihre Skireise und die verfassten Texte handeln von ihren Vorbereitungen, der Reiseroute, den Etappen, der Ausrüstung, dem Gepäck, dem Essen, den Campingplätzen, der Fußpflege, den Skiern, dem Wetter, der Sonne, der Kälte, dem Schlaf sowie den Tieren und der Natur.Die körperliche Anstrengung wurde wissenschaftlich untersucht und führte zu vier Veröffentlichungen, die nachfolgend als verschiedene Einträge in digitales wissenschaftliches Archiv (DiVA) aufgeführt sind:Schantz, Peter et al. 1982. Training-induced increase in myofibrillar ATPase intermediate fibers in human skeletal muscle. Muscle & Nerve 5:628-636.Schantz, Peter et al. 1983. Adaptation of human skeletal muscle to endurance training of long duration. Clin. Physiol 3:141-151.Schantz, Peter. 1986. Plasticity of human skeletal muscle - with special reference to effectsof physical training on enzyme levels of the NADH shuttles and phenotypicexpression of slow and fast isoforms of myofibrillar proteins, Acta Physiol Scand, vol 128, Suppl.558, pp 1-64. Schantz, Peter. 1980. Långtur - om 150 turåkning längs den svenska fjällkedjan. Stockholm: Trygg-Hansa.
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| 6. |
- Fernström, Erik
(författare)
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Fjällmarsch Trantre
- 1979
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Annan publikation (populärvet., debatt m.m.)abstract
- Under vintern 1978 genomfördes Fjällmarsch TranTre på skidor av de sex unga män som har skrivit denna dokumentation av skidturen. Den startade den 1 mars i Transtrand och fortsatte längs den svenska fjällkedjan upp till Treriksröset. Därefter färdades de söderut till tågstationen Torne träsk, som de kom till den 27 april. De hade då tillryggalagt en sträcka på 1500 km under 50 dagar.Texterna handlar om deltagarna, deras förberedelser, färdvägen, dagsetapperna, utrustningen, packningen, maten, lägerplatserna, fotvården, skidorna, vädret, solen, kylan, sömnen, samt djuren och naturen. Det fysiska arbetet studerades vetenskapligt och gav upphov till fyra publikationer som anges nedan som olika poster i DiVA:Schantz, Peter et al. 1982. Training-induced increase in myofibrillar ATPase intermediate fibers in human skeletal muscle. Muscle & Nerve 5:628-636.Schantz, Peter et al. 1983. Adaptation of human skeletal muscle to endurance training of long duration. Clin. Physiol 3:141-151.Schantz, Peter. 1986. Plasticity of human skeletal muscle - with special reference to effectsof physical training on enzyme levels of the NADH shuttles and phenotypicexpression of slow and fast isoforms of myofibrillar proteins, Acta Physiol Scand, vol 128, Suppl.558, pp 1-64. Schantz, Peter. 1980. Långtur - om 150 turåkning längs den svenska fjällkedjan. Stockholm: Trygg-Hansa.
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| 7. |
- Johansson, Pegah, 1978, et al.
(författare)
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In-solution staining and arraying method for the immunofluorescence detection of γH2AX foci optimized for clinical applications.
- 2011
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Ingår i: BioTechniques. - : Future Science Ltd. - 1940-9818 .- 0736-6205. ; 51:3, s. 185-9
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Tidskriftsartikel (refereegranskat)abstract
- Immunofluorescence quantification of γH2AX foci is a powerful approach to quantify DNA double-strand breaks induced by cancer therapy or accidental exposure to ionizing radiation. Here we report a modification to the γH2AX immunofluorescence labeling method, whereby cells are stained in-solution before being spotted and fixed onto microscope slides. Our modified method allows arraying of 16 patient samples/slide ready for foci counting in 2 h and demonstrated reliably detection of γH2AX foci in mononuclear cells prepared from patients who had undergone radiation therapy.
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| 8. |
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| 9. |
- Paramastri, Yasmina, et al.
(författare)
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Urinary and fecal immunoglobulin A, cortisol and 11-17 dioxoandrostanes, and serum cortisol in metabolic cage housed female cynomolgus monkeys (Macaca fascicularis)
- 2007
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Ingår i: Journal of Medical Primatology. - : Wiley. - 0047-2565 .- 1600-0684. ; 36:6, s. 355-364
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Tidskriftsartikel (refereegranskat)abstract
- Background and methods: Quantitative enzyme-immunoassays of urinary and fecal immunoglobulin A (IgA), cortisol and 11-17-dioxoandrostanes (11,17-DOA), and serum cortisol in eight metabolic-cage-housed female cynomolgus monkeys were performed. The monkeys were divided into two groups, B and NB. Group B animals were blood sampled every 6 hours, whereas Group NB animals were not handled/blood sampled. Results: No differences were recorded between the amounts of feces and urine excreted by the two groups. Group B animals excreted more urinary cortisol than did Group NB animals indicating that restraint-blood sampling resulted in a stress response. Excreted amounts of IgA and 11,17-DOA (urine and feces) did not differ between the groups. Conclusion: Urinary cortisol was a reliable marker of the stress associated with repeated blood sampling. Declining amounts of excreted urinary cortisol indicated that cynomolgus monkeys acclimated quickly to repeated blood sampling in metabolism cages. Within and between animal variation in amounts of feces voided demonstrated the importance of expressing fecal markers as 'amounts excreted per time unit per kg body weight' rather than just measuring the concentrations in fecal samples.
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| 10. |
- Rydén, Isabelle, et al.
(författare)
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Neuropsychological functioning in childhood cancer survivors following cranial radiotherapy - results from a long-term follow-up clinic
- 2022
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Ingår i: Neurocase. - : Informa UK Limited. - 1355-4794 .- 1465-3656. ; 28:2, s. 163-172
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Tidskriftsartikel (refereegranskat)abstract
- Treatment of malignant childhood posterior fossa tumors (CPFT) often includes surgical resection and craniospinal radiotherapy (CSI). Nasopharyngeal tumors in childhood (CNPHT) are often treated with surgery and radiotherapy (RT), leading to incidental brain irradiation. RT to the developing brain is associated with risks for cognitive impairments. We studied cognitive functioning, health-related quality of life (HRQOL), fatigue, and psychological distress, in adult survivors of CPFT and CNPHT, representing two groups, which had received high and low radiation dose-exposure to the brain, respectively. Cognitive tests were used to compare CPFT (n = 12) and CNPHT (n = 7) survivors to matched healthy controls (n = 28). HRQOL data was compared to the general population (GP) (n = 1415-1459). Average follow-up was 23 (CPFT) and 19 years (CNPHT). CPFT survivors had significant deficits in all cognitive domains. CNPHT survivors showed results below the control group but differed statistically only on one executive test. HRQOL-ratings indicated that both groups had similar self-reported cognitive problems. CPFT survivors reported more emotional problems and fatigue. Anxiety was seen in both CPFT and CNPHT survivors. This study confirmed long-term cognitive sequelae after RT in adult survivors of CPFT,and possible RT-induced cognitive deficits in adult CNPHT survivors.
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