| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
|
|
| 11. |
|
|
| 12. |
- Olsson, E., et al.
(författare)
-
Ultra-high field magnetic resonance imaging parameter mapping in the posterior horn of ex vivo human menisci
- 2019
-
Ingår i: Osteoarthritis and Cartilage. - : Elsevier BV. - 1063-4584. ; 27:3, s. 476-483
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: To investigate the relationship between meniscus magnetic resonance (MR) relaxation parameters and meniscus degradation through quantitative imaging of ex vivo posterior horns of menisci from subjects with and without knee osteoarthritis (OA). Design: We sampled medial and lateral menisci from ten medial compartment knee OA patients (mean age 63 years) undergoing total knee replacement and from ten deceased donors (references, mean age 51 years). MR relaxation parameters T2*, T2 and T1 of the posterior horn were measured at a 9.4 T scanner. Comparisons were made between OA patients and references (with adjustment for age) as well as between medial and lateral menisci from the same knees. Results: Mean values (standard deviation) of mean T2* were 13 (3.8), 6.9 (2.3), 7.2 (1.9) and 7.2 (1.7) ms for the medial and lateral patient menisci and the medial and lateral reference menisci, respectively. Corresponding values were 17 (3.7), 9.0 (2.2), 12 (4) and 9.0 (1.3) ms for T2 and 1810 (150), 1630 (30), 1580 (90) and 1560 (50) ms for T1. All three relaxation times were significantly longer in medial OA menisci compared to the other groups. Among medial reference menisci, relaxation times (mainly T1) tended to increase with age. Conclusions: MR relaxation times T2*, T2 and T1 in the posterior horn are longer in the medial menisci of patients with end-stage medial compartment knee OA compared to the corresponding lateral menisci and to reference menisci. The meniscus seems to undergo intrasubstance alterations related to both OA and ageing.
|
|
| 13. |
- Björkman, Sven E., et al.
(författare)
-
In vivo recovery of factor VIII and factor IX : intra- and interindividual variance in a clinical setting
- 2007
-
Ingår i: Haemophilia. - : Wiley. - 1351-8216 .- 1365-2516. ; 13:1, s. 2-8
-
Tidskriftsartikel (refereegranskat)abstract
- In vivo recovery (IVR) is traditionally used as a parameter to characterize the pharmacokinetic properties of coagulation factors. It has also been suggested that dosing of factor VIII (FVIII) and factor IX (FIX) can be adjusted according to the need of the individual patient, based on an individually determined IVR value. This approach, however, requires that the individual IVR value is more reliably representative for the patient than the mean value in the population, i.e. that there is less variance within than between the individuals. The aim of this investigation was to compare intra- and interindividual variance in IVR (as U dL−1 per U kg−1) for FVIII and plasma-derived FIX in a cohort of non-bleeding patients with haemophilia. The data were collected retrospectively from six clinical studies, yielding 297 IVR determinations in 50 patients with haemophilia A and 93 determinations in 13 patients with haemophilia B. For FVIII, the mean variance within patients exceeded the between-patient variance. Thus, an individually determined IVR value is apparently no more informative than an average, or population, value for the dosing of FVIII. There was no apparent relationship between IVR and age of the patient (1.5–67 years). For FIX, the mean variance within patients was lower than the between-patient variance, and there was a significant positive relationship between IVR and age (13–69 years). From these data, it seems probable that using an individual IVR confers little advantage in comparison to using an age-specific population mean value. Dose tailoring of coagulation factor treatment has been applied successfully after determination of the entire single-dose curve of FVIII:C or FIX:C in the patient and calculation of the relevant pharmacokinetic parameters. However, the findings presented here do not support the assumption that dosing of FVIII or FIX can be individualized on the basis of a clinically determined IVR value.
|
|
| 14. |
- Eriksson, Leif, 1970, et al.
(författare)
-
ALOS PALSAR Calibration and Validation Results from Sweden
- 2007
-
Ingår i: IEEE Geoscience and Remote Sensing Symposium (IGARSS 2007), Barcelona, Spain, July 23-27, 2007. ; DOI: 10.1109/IGARSS.2007.4423115, s. 1589-1592
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- In 2006 calibration activities for ALOS PALSAR wereconducted in Sweden. Four five-metre trihedral corner reflectorsand three smaller dihedral reflectors were deployed and operatedduring eight months. 23 PALSAR scenes were acquired over thecalibration site allowing an evaluation of the quality and temporalstability of the data. Results show that the co-polarized datahave been stable during the whole calibration period with variationsin the trihedral responses lower than 0.7 dB. The measuredresolution in azimuth was 4.4 m and in slant range 4.7 m forsingle polarization images and 9.5 m for polarimetric data. Forthe cross-polarized data large variations in the dihedral responseswere found. It is assumed that this is caused by a larger sensitivityto pointing errors. For the polarimetric data, estimation ofFaraday rotation gave values ranging from 0.1º to 3º.
|
|
| 15. |
- Folkesson, E., et al.
(författare)
-
Proteomic comparison of osteoarthritic and reference human menisci using data-independent acquisition mass spectrometry
- 2020
-
Ingår i: Osteoarthritis and Cartilage. - : Elsevier BV. - 1063-4584. ; 28:8, s. 1092-1101
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: Recent research in knee osteoarthritis (OA) highlights the role of the meniscus in OA pathology. Our aim was to compare the proteomes of medial and lateral menisci from end-stage medial compartment knee OA patients, with reference menisci from knee-healthy deceased donors, using mass spectrometry. Design: Tissue plugs of Ø3 mm were obtained from the posterior horns of the lateral and medial menisci from one knee of 10 knee-healthy deceased donors and 10 patients undergoing knee replacement. Proteins were extracted and prepared for mass spectrometric analysis. Statistical analysis was conducted on abundance data that was log2-transformed, using a linear mixed effects model and evaluated using pathway analysis. Results: We identified a total of 835 proteins in all samples, of which 331 were included in the statistical analysis. The largest differences could be seen between the medial menisci from OA patients and references, with most proteins showing higher intensities in the medial menisci from OA patients. Several matrix proteins, e.g., matrix metalloproteinase 3 (MMP3) (4.3 times higher values [95%CI 1.8, 10.6]), TIMP1 (3.5 [1.4, 8.5]), asporin (4.1 [1.7, 10.0]) and versican (4.4 [1.8, 10.9]), all showed higher abundance in medial menisci from OA patients compared to medial reference menisci. OA medial menisci also showed increased activation of several pathways involved in inflammation. Conclusion: An increase in protein abundance for proteins such as MMP and TIMP1 in the medial menisci from OA patients suggests simultaneous activation of both catabolic and anabolic processes that warrants further attention.
|
|
| 16. |
|
|
| 17. |
- Folkesson, R, et al.
(författare)
-
Alzheimer transgenic models
- 2002
-
Ingår i: CURRENT OPINION IN PSYCHIATRY. - : Ovid Technologies (Wolters Kluwer Health). - 0951-7367. ; 15:4, s. 433-439
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
|
|
| 18. |
- Fransson, J.E.S., et al.
(författare)
-
Detection of clear-cuts using ALOS PALSAR satellite images
- 2008
-
Ingår i: Proceedings of the European Conference on Synthetic Aperture Radar, EUSAR. - 2197-4403. ; 1-4
-
Konferensbidrag (refereegranskat)abstract
- The objective of this study is to make a first evaluation of the possibilities to detect forest clear-cuts using high-resolution ALOS PALSAR FBD (Fine Beam Dual polarization) satellite images. New operational applications for mapping of changes in forest cover are of interest for government authorities in Sweden and in other countries with similar needs. The study was conducted in southern Sweden and included seven old coniferous stands located on flat terrain. Three of the stands were clear-felled and the remaining stands were left untreated for reference. Altogether, six PALSAR FBD images (look angle 34.3°, HH- and HV-polarization) acquired during the summer and fall seasons were analyzed. The difference in backscattering coefficient between the reference and the clear-felled stands was on average 2.4 dB and 2.9 dB for the HH- and HV-polarization, respectively. When comparing the backscattering coefficient before and after clear-felling the drop was found to be 1.7 dB and 2.3 dB for the HH- and HV-polarization, respectively.
|
|
| 19. |
- Fransson, J.E.S., et al.
(författare)
-
Mapping of Wind-Thrown Forests Using VHF/UHF SAR Images
- 2007
-
Ingår i: IEEE 2007 International Geoscience and Remote Sensing Symposium (IGARSS 2007), Barcelona, Spain, July 23-27 2007. ; DOI: 10.1109/IGARSS.2007.4423313, s. 2350-2353
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- SAR images from the Swedish airborne CARABAS-II and LORA systems have been visually analyzed over simulated wind-thrown forest at both single tree and stand level. In ideal conditions, the results show that LORA is more accurate than CARABAS-II at detecting wind-thrown trees, regardless of tree size and direction of the fallen trees relative to flight heading. Furthermore, the visible single trees in the LORA images appeared more distinct than in the CARABAS-II images, which could be explained by the high resolution in the LORA images. Based on visual interpretation, it is likely that the detection of wind-thrown forests could be improved using VHF/UHF SAR images acquired both prior to and after a storm event.
|
|
| 20. |
- Karjalainen, V. P., et al.
(författare)
-
Quantitative three-dimensional collagen orientation analysis of human meniscus posterior horn in health and osteoarthritis using micro-computed tomography
- 2021
-
Ingår i: Osteoarthritis and Cartilage. - : Elsevier BV. - 1063-4584. ; 29:5, s. 762-772
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: Knee osteoarthritis (OA) is associated with meniscal degeneration that may involve disorganization of the meniscal collagen fiber network. Our aims were to quantitatively analyze the microstructural organization of human meniscus samples in 3D using micro-computed tomography (μCT), and to compare the local microstructural organization between OA and donor samples. Method: We collected posterior horns of both medial and lateral human menisci from 10 end-stage medial compartment knee OA patients undergoing total knee replacement (medial & lateral OA) and 10 deceased donors without knee OA (medial & lateral donor). Posterior horns were dissected and fixed in formalin, dehydrated in ascending ethanol concentrations, treated with hexamethyldisilazane (HMDS), and imaged with μCT. We performed local orientation analysis of collagenous microstructure in 3D by calculating structure tensors from greyscale gradients within selected integration window to determine the polar angle for each voxel. Results: In donor samples, meniscus bundles were aligned circumferentially around the inner border of meniscus. In medial OA menisci, the organized structure of collagen network was lost, and main orientation was shifted away from the circumferential alignment. Quantitatively, medial OA menisci had the lowest mean orientation angle compared to all groups, −24° (95%CI -31 to −18) vs medial donor and −25° (95%CI -34 to −15) vs lateral OA. Conclusions: HMDS-based μCT imaging enabled quantitative analysis of meniscal collagen fiber bundles and their orientations in 3D. In human medial OA menisci, the collagen disorganization was profound with overall lower orientation angles, suggesting collagenous microstructure disorganization as an important part of meniscus degradation.
|
|
| 21. |
- Kestilä, I., et al.
(författare)
-
Three-dimensional microstructure of human meniscus posterior horn in health and osteoarthritis
- 2019
-
Ingår i: Osteoarthritis and Cartilage. - : Elsevier BV. - 1063-4584. ; 27:22, s. 1790-1799
-
Tidskriftsartikel (refereegranskat)abstract
- Objective: To develop and perform ex vivo 3D imaging of meniscus posterior horn microstructure using micro-computed tomography (μCT), and to compare specimens from healthy references against end-stage osteoarthritis (OA) using conventional section-based histology and qualitative μCT. Design: We retrieved human medial and lateral menisci from 10 deceased donors without knee OA (healthy references) and medial and lateral menisci from 10 patients having total knee replacement for medial compartment OA. Meniscal posterior horns were dissected and fixed in formalin. One subsection underwent hexamethyldisilazane (HMDS) treatment and μCT imaging. Pauli's histopathological scoring was performed for 3 other subsections. The differences in histopathological scores were estimated using mixed linear regression, resulting in fixed effects estimates for within-knee comparisons and adjusted for age and body mass index for between-subjects comparisons. Results: 3D visualization with μCT qualitatively revealed similar microstructural changes in the posterior horns as conventional histology. The mean histopathological score was higher for medial menisci from OA knees vs both medial reference menisci (mean difference [95% CI], 3.9 [2.6,5.3]), and lateral menisci from OA knees (3.9 [2.9,5.0]). The scores were similar between lateral menisci from OA knees and lateral reference menisci (0.8 [−0.6,2.2]), and between medial and lateral reference menisci (0.8 [−0.3,1.9]). Conclusions: HMDS-based μCT protocol allows unique 3D visualization of meniscus microstructures. Posterior horns of medial menisci from medial compartment OA knees had higher histopathological scores than both the lateral posterior horns from the same OA knees and medial reference menisci, suggesting a strong association between meniscus degradation and unicompartmental knee OA.
|
|
| 22. |
- Ketels, Christian, et al.
(författare)
-
Clusters in the EU-10 new member countries
- 2007
-
Ingår i: INNOVATION CLUSTERS IN THE 10 NEW MEMBER STATES OF THE EUROPEAN UNION. - Brussels : European Communities. - 9279031961 ; , s. 49-62
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)
|
|
| 23. |
|
|
| 24. |
|
|
| 25. |
|
|
| 26. |
|
|
| 27. |
|
|
| 28. |
|
|
| 29. |
|
|
| 30. |
|
|
| 31. |
|
|
| 32. |
|
|
| 33. |
|
|
| 34. |
|
|
| 35. |
|
|
| 36. |
- Silva, CS, et al.
(författare)
-
High Dimensional Immune Profiling Reveals Different Response Patterns in Active and Latent Tuberculosis Following Stimulation With Mycobacterial Glycolipids
- 2021
-
Ingår i: Frontiers in immunology. - : Frontiers Media SA. - 1664-3224. ; 12, s. 727300-
-
Tidskriftsartikel (refereegranskat)abstract
- Upon infection withMycobacterium tuberculosis(Mtb) the host immune response might clear the bacteria, control its growth leading to latent tuberculosis (LTB), or fail to control its growth resulting in active TB (ATB). There is however no clear understanding of the features underlying a more or less effective response. Mtb glycolipids are abundant in the bacterial cell envelope and modulate the immune response to Mtb, but the patterns of response to glycolipids are still underexplored. To identify the CD45+leukocyte activation landscape induced by Mtb glycolipids in peripheral blood of ATB and LTB, we performed a detailed assessment of the immune response of PBMCs to the Mtb glycolipids lipoarabinomannan (LAM) and its biosynthetic precursor phosphatidyl-inositol mannoside (PIM), and purified-protein derivate (PPD). At 24 h of stimulation, cell profiling and secretome analysis was done using mass cytometry and high-multiplex immunoassay. PIM induced a diverse cytokine response, mainly affecting antigen-presenting cells to produce both pro-inflammatory and anti-inflammatory cytokines, but not IFN-γ, contrasting with PPD that was a strong inducer of IFN-γ. The effect of PIM on the antigen-presenting cells was partly TLR2-dependent. Expansion of monocyte subsets in response to PIM or LAM was reduced primarily in LTB as compared to healthy controls, suggesting a hyporesponsive/tolerance pattern derived from Mtb infection.
|
|
| 37. |
|
|
| 38. |
|
|
| 39. |
|
|
| 40. |
- Telemo, E., et al.
(författare)
-
Maternal dietary antigens and the immune response in the offspring of the guinea-pig
- 1987
-
Ingår i: Immunology. - 0019-2805. ; 62:1, s. 35-38
-
Tidskriftsartikel (refereegranskat)abstract
- Guinea-pig dams and their litters were raised on either a cow's milk protein-containing diet (MCD) or a milk-free diet (MFD). At 8 weeks of age all litters were challenged i.p. with 50 μg milk whey-protein concentrate (v67) and 100 mg A1(OH)3 in saline. The immune response was estimated 2 weeks later as the serum IgG antibody titres against V67, β-lactoglobulin (β-LG) and α-lactalbumin (α-LA) using enzyme-linked immunosorbent assay (ELISA) and the tracheal Schulze-Dale response to these antigens. Feeding milk protein antigen to dams from birth and during pregnancy induces antigen-specific hyporesponsiveness (tolerance) in their offspring, despite no direct contact between the offspring and the milk proteins. Tolerance seems to be induced by the antigen itself since withdrawal of the MCD 10 days before delivery reduced tolerance in the offspring. No tolerance was produced in the offspring of dams fed the antigen from 3 months of age (adult). β-LG appears to be a major antigen in milk whey while α-LA is a minor one since there was almost no antibody or tracheal response to α-LA in any of the animals tested. The results indicate that maternal antigen experience and antigens present during pregnancy are important for the subsequent immune response to these antigens in offspring.
|
|
| 41. |
|
|
| 42. |
|
|
| 43. |
|
|
