| 1. |
- Rosendal, Ebba, et al.
(författare)
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Serine Protease Inhibitors Restrict Host Susceptibility to SARS-CoV-2 Infections
- 2022
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Ingår i: mBio. - : American Society for Microbiology. - 2161-2129 .- 2150-7511. ; 13:3
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Tidskriftsartikel (refereegranskat)abstract
- The coronavirus disease 2019, COVID-19, is a complex disease with a wide range of symptoms from asymptomatic infections to severe acute respiratory syndrome with lethal outcome. Individual factors such as age, sex, and comorbidities increase the risk for severe infections, but other aspects, such as genetic variations, are also likely to affect the susceptibility to SARS-CoV-2 infection and disease severity. Here, we used a human 3D lung cell model based on primary cells derived from multiple donors to identity host factors that regulate SARS-CoV-2 infection. With a transcriptomics-based approach, we found that less susceptible donors show a higher expression level of serine protease inhibitors SERPINA1, SERPINE1, and SERPINE2, identifying variation in cellular serpin levels as restricting host factors for SARS-CoV-2 infection. We pinpoint their antiviral mechanism of action to inhibition of the cellular serine protease, TMPRSS2, thereby preventing cleavage of the viral spike protein and TMPRSS2-mediated entry into the target cells. By means of single-cell RNA sequencing, we further locate the expression of the individual serpins to basal, ciliated, club, and goblet cells. Our results add to the importance of genetic variations as determinants for SARS-CoV-2 susceptibility and suggest that genetic deficiencies of cellular serpins might represent risk factors for severe COVID-19. Our study further highlights TMPRSS2 as a promising target for antiviral intervention and opens the door for the usage of locally administered serpins as a treatment against COVID-19.
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| 2. |
- Gröning, Remigius, et al.
(författare)
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Intravenous immunoglobulin therapy for COVID-19 in immunocompromised patients : A retrospective cohort study
- 2024
-
Ingår i: International Journal of Infectious Diseases. - : Elsevier. - 1201-9712 .- 1878-3511. ; 144
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: To investigate the effectiveness of intravenous immunoglobulin (IVIG) as treatment for COVID-19 in immunocompromised patients. Methods: This retrospective study investigated outcomes for immunocompromised, vaccine non-responsive, patients that between September 2022 and April 2023 received IVIG as treatment for COVID-19 in the region of Västerbotten, Sweden. We analyzed clinical data, viral load, and anti-SARS-CoV-2 IgG binding and neutralization levels of patient serum samples and IVIG production batches. Primary and secondary outcomes were clinical cure and viral clearance, respectively.Results: Sixteen patients were analyzed. After a median COVID-19 duration of 4 weeks, a median 60 g IVIG infusion increased SARS-CoV-2 binding and neutralizing antibody levels, with broad in vitro activity against tested variants. The treatment resulted in abrogation of viremia in all patients and general improvement in 15 survivors that all met the primary endpoint. Thirteen patients met the secondary endpoint at follow-up after a median of four months. Two subjects with persistent SARS-CoV-2 carriage relapsed but were successfully retreated with IVIG.Conclusions: Antibodies in IVIG efficiently neutralized several SARS-CoV-2 variants. Treatment with IVIG was associated with clinical cure and viral clearance in immunocompromised patients. Our data suggests that IVIG could be a novel treatment alternative for COVID-19 for this patient category.
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| 3. |
- Näslund, Johan, 1954-, et al.
(författare)
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Studerandes syn på grupparbete
- 2020
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Ingår i: Handbok för grupparbete. - Lund : Studentlitteratur AB. - 9789144132556 ; , s. 289-305
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 4. |
- Ahmad, Irma, et al.
(författare)
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High prevalence of persistent symptoms and reduced health-related quality of life 6 months after COVID-19
- 2023
-
Ingår i: Frontiers In Public Health. - : Frontiers Media S.A.. - 2296-2565. ; 11
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: The long-term sequelae after COVID-19 constitute a challenge to public health and increased knowledge is needed. We investigated the prevalence of self-reported persistent symptoms and reduced health-related quality of life (HRQoL) in relation to functional exercise capacity, 6 months after infection, and explored risk factors for COVID-19 sequalae.METHODS: This was a prospective, multicenter, cohort study including 434 patients. At 6 months, physical exercise capacity was assessed by a 1-minute sit-to-stand test (1MSTST) and persistent symptoms were reported and HRQoL was evaluated through the EuroQol 5-level 5-dimension (EQ-5D-5L) questionnaire. Patients with both persistent symptoms and reduced HRQoL were classified into a new definition of post-acute COVID syndrome, PACS+. Risk factors for developing persistent symptoms, reduced HRQoL and PACS+ were identified by multivariable Poisson regression.RESULTS: Persistent symptoms were experienced by 79% of hospitalized, and 59% of non-hospitalized patients at 6 months. Hospitalized patients had a higher prevalence of self-assessed reduced overall health (28 vs. 12%) and PACS+ (31 vs. 11%). PACS+ was associated with reduced exercise capacity but not with abnormal pulse/desaturation during 1MSTST. Hospitalization was the most important independent risk factor for developing persistent symptoms, reduced overall health and PACS+.CONCLUSION: Persistent symptoms and reduced HRQoL are common among COVID-19 survivors, but abnormal pulse and peripheral saturation during exercise could not distinguish patients with PACS+. Patients with severe infection requiring hospitalization were more likely to develop PACS+, hence these patients should be prioritized for clinical follow-up after COVID-19.
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| 5. |
- Björsell, Tove, et al.
(författare)
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Risk factors for impaired respiratory function post COVID-19 : A prospective cohort study of nonhospitalized and hospitalized patients
- 2023
-
Ingår i: Journal of Internal Medicine. - : Wiley-Blackwell Publishing Inc.. - 0954-6820 .- 1365-2796. ; 293:5, s. 600-614
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Severe COVID-19 increases the risk for long-term respiratory impairment, but data after mild COVID-19 are scarce. Our aims were to determine risk factors for reduced respiratory function 3-6 months after COVID-19 infection and to investigate if reduced respiratory function would relate to impairment of exercise performance and breathlessness.METHODS: Patients with COVID-19 were enrolled at the University Hospitals of Umeå and Örebro, and Karlstad Central Hospital, Sweden. Disease severity was defined as mild (nonhospitalized), moderate (hospitalized with or without oxygen treatment), and severe (intensive care). Spirometry, including diffusion capacity (DLCO ), was performed 3-6 months after hospital discharge or study enrollment (for nonhospitalized patients). Breathlessness (defined as ≥1 according to the modified Medical Research Council scale) and functional exercise capacity (1-min sit-to-stand test; 1-MSTST) were assessed.RESULTS: Between April 2020 and May 2021, 337 patients were enrolled in the study. Forced vital capacity and DLCO were significantly lower in patients with severe COVID-19. Among hospitalized patients, 20% had reduced DLCO , versus 4% in nonhospitalized. Breathlessness was found in 40.6% of the participants and was associated with impaired DLCO . A pathological desaturation or heart rate response was observed in 17% of participants during the 1-MSTST. However, this response was not associated with reduced DLCO .CONCLUSION: Reduced DLCO was the major respiratory impairment 3-6 months following COVID-19, with hospitalization as the most important risk factor. The lack of association between impaired DLCO and pathological physiological responses to exertion suggests that these physiological responses are not primarily related to decreased lung function.
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| 6. |
- Cagigi, Alberto, et al.
(författare)
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Airway antibodies emerge according to COVID-19 severity and wane rapidly but reappear after SARS-CoV-2 vaccination
- 2021
-
Ingår i: JCI Insight. - : American Society for Clinical Investigation. - 2379-3708. ; 6:22
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Tidskriftsartikel (refereegranskat)abstract
- Understanding the presence and durability of antibodies against SARS-CoV-2 in the airways is required to provide insights into the ability of individuals to neutralize the virus locally and prevent viral spread. Here, we longitudinally assessed both systemic and airway immune responses upon SARS-CoV-2 infection in a clinically well-characterized cohort of 147 infected individuals representing the full spectrum of COVID-19 severity, from asymptomatic infection to fatal disease. In addition, we evaluated how SARS-CoV-2 vaccination influenced the antibody responses in a subset of these individuals during convalescence as compared with naive individuals. Not only systemic but also airway antibody responses correlated with the degree of COVID-19 disease severity. However, although systemic IgG levels were durable for up to 8 months, airway IgG and IgA declined significantly within 3 months. After vaccination, there was an increase in both systemic and airway antibodies, in particular IgG, often exceeding the levels found during acute disease. In contrast, naive individuals showed low airway antibodies after vaccination. In the former COVID-19 patients, airway antibody levels were significantly elevated after the boost vaccination, highlighting the importance of prime and boost vaccinations for previously infected individuals to obtain optimal mucosal protection.
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| 7. |
- Carrasco, Anna, et al.
(författare)
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The Tonsil Lymphocyte Landscape in Pediatric Tonsil Hyperplasia and Obstructive Sleep Apnea
- 2021
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Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- Tonsil hyperplasia is the most common cause of pediatric obstructive sleep apnea (OSA). Despite the growing knowledge in tissue immunology of tonsils, the immunopathology driving tonsil hyperplasia and OSA remains unknown. Here we used multi-parametric flow cytometry to analyze the composition and phenotype of tonsillar innate lymphoid cells (ILCs), T cells, and B cells from pediatric patients with OSA, who had previous polysomnography. Unbiased clustering analysis was used to delineate and compare lymphocyte heterogeneity between two patient groups: children with small tonsils and moderate OSA (n = 6) or large tonsils and very severe OSA (n = 13). We detected disturbed ILC and B cell proportions in patients with large tonsils, characterized by an increase in the frequency of naive CD27(-)CD21(hi) B cells and a relative reduction of ILCs. The enrichment of naive B cells was not commensurate with elevated Ki67 expression, suggesting defective differentiation and/or migration rather than cellular proliferation to be the causative mechanism. Finally, yet importantly, we provide the flow cytometry data to be used as a resource for additional translational studies aimed at investigating the immunological mechanisms of pediatric tonsil hyperplasia and OSA.
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| 8. |
- Dernstedt, Andy, et al.
(författare)
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Regulation of Decay Accelerating Factor Primes Human Germinal Center B Cells for Phagocytosis
- 2021
-
Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 11
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Tidskriftsartikel (refereegranskat)abstract
- Germinal centers (GC) are sites for extensive B cell proliferation and homeostasis is maintained by programmed cell death. The complement regulatory protein Decay Accelerating Factor (DAF) blocks complement deposition on host cells and therefore also phagocytosis of cells. Here, we show that B cells downregulate DAF upon BCR engagement and that T cell-dependent stimuli preferentially led to activation of DAF(lo) B cells. Consistent with this, a majority of light and dark zone GC B cells were DAF(lo) and susceptible to complement-dependent phagocytosis, as compared with DAF(hi) GC B cells. We could also show that the DAF(hi) GC B cell subset had increased expression of the plasma cell marker Blimp-1. DAF expression was also modulated during B cell hematopoiesis in the human bone marrow. Collectively, our results reveal a novel role of DAF to pre-prime activated human B cells for phagocytosis prior to apoptosis.
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| 9. |
- Eriksson, Ljusk Ola, et al.
(författare)
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Impacts of Long-term Strategies for the Swedish Forest Sector: Analyses with the BioFrame Integrated Modelling Framework
- 2024
-
Ingår i: Journal of Forest Economics. - 1104-6899 .- 1618-1530. ; 39:2, s. 137-185
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Tidskriftsartikel (refereegranskat)abstract
- The European Union (EU) does not have a common forestry policy but EU policies can indirectly affect the forest sector. This study departs from the EU “Fit for 55” package of legislation and uses a forest sector model to simulate and analyze three responses in the Swedish forest sector (2020–2100) to policy initiatives addressing climate change and biodiversity: (i) increasing the area of set-asides with 50%; (ii) prohibiting harvest of old forest (>120/140 years of age); and (iii) extending the minimum allowed age for final harvest with 30%. Results indicate that, while all three responses can reduce net carbon emissions compared to business-as-usual, extension of the minimum allowed age for final harvest reduces emissions the most. In general, the effects on net carbon emissions are highly correlated with the level of harvest. Increasing the area of set-asides and prohibiting old forest harvest help preserve old forest better than both business-as-usual and final felling age regulation. Longer-term results are uncertain as policies and technology development can radically change biomass use, product portfolios and displacement effects.
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| 10. |
- Forsell, Eskil, et al.
(författare)
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Predicting replication outcomes in the Many Labs 2 study
- 2019
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Ingår i: Journal of Economic Psychology. - : Elsevier. - 1872-7719 .- 0167-4870. ; 75:Part A SI
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Tidskriftsartikel (refereegranskat)abstract
- Understanding and improving reproducibility is crucial for scientific progress. Prediction markets and related methods of eliciting peer beliefs are promising tools to predict replication outcomes. We invited researchers in the field of psychology to judge the replicability of 24 studies replicated in the large scale Many Labs 2 project. We elicited peer beliefs in prediction markets and surveys about two replication success metrics: the probability that the replication yields a statistically significant effect in the original direction (p < 0.001), and the relative effect size of the replication. The prediction markets correctly predicted 75% of the replication outcomes, and were highly correlated with the replication outcomes. Survey beliefs were also significantly correlated with replication outcomes, but had larger prediction errors. The prediction markets for relative effect sizes attracted little trading and thus did not work well. The survey beliefs about relative effect sizes performed better and were significantly correlated with observed relative effect sizes. The results suggest that replication outcomes can be predicted and that the elicitation of peer beliefs can increase our knowledge about scientific reproducibility and the dynamics of hypothesis testing.
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| 11. |
- Forsell, Johan, 1980-
(författare)
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Assessment of abilities and knowledge learned in group
- 2017
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Ingår i: 18th Biennial Conference on Teachers and Teaching. - 9788469744185
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Konferensbidrag (refereegranskat)abstract
- Working cooperatively in groups is proven by numerous of studies to have positive effects on students learning. Researchers also describe how learning in groups comes with certain challenges and dilemmas.Teachers find it challenging to assess abilities and knowledge learned within a group. Students’, on the other hand, may consider unfairness as a problem when teachers assess individual abilities and knowledge based on the whole group. This ongoing research projects aim is to investigate how a short educational intervention can influence teachers´ understanding of assessment of abilities and knowledge learned in groups and how to develop new strategies to cope differently with the problems and dilemmas tied to the subject.The study is carried out in Swedish upper secondary high schools (students 15-18 years). The research includes six teachers who realize the same group task in their classes. Three of the teachers will function as a control group and get the intervention after the group project is carried out. The main content of the educational intervention is theories of how to assess abilities and knowledge learned in groups.Data is collected by individual interviews with the teachers and group interviews with students before and after the intervention and group project are carried out. Data will also be collected by video observation during the group project. This study will contribute with new important insights and knowledge of how teachers assess abilities and knowledge learned in groups and how they can develop strategies to cope with the challenges that comes with that.
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| 12. |
- Forsell, Johan
(författare)
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Development of photoreceptor organs in teleosts, Molecular and immunocytochemical studies
- 2000
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Many vertebrate animals are known to have multiple sites for photoreception that are not involved in vision. Extraretinal photoreception is indicated to play a crucial role in early development, physiology and behavior, as well as in later biological processes. This thesis encompasses four studies investigating the neuromorphological, neurochemical and molecular basis for extraretinal photosensitivity in teleosts (bony fishes) during development. The first study, investigating developing Atlantic halibut, identified the pineal organ as the only photoreceptor organ with a capacity to mediate photic information crucial for embryonic behavioral processes such as vertical migration and hatching. In the second study, Atlantic herring and cod were investigated, as they have very different early life strategies: during early development the herring is demersal (bottom-dwelling), wheras the cod is pelagic. In both species, extraretinal photosensory organs develop before the retina. The temporal difference was more pronounced in the demersal herring embryos than in the pelagic cod. Furthermore, herring embryos possess additional "deep" photoreceptors in the brain, previously not shown in developing teleosts. In the third study, two opsin (the protein part of the photopigment) genes were isolated from the halibut pineal, and characterized as coding for a UV-sensitive and a green-sensitive opsin. Both are expressed in the embryonic pineal organ. At first, UV-sensitive opsin predominates, but during subsequent development green-sensitive opsin prevails, possibly reflecting the switch from a pelagic to a demersal life. Finally, in the fourth study the presence of UV-sensitive opsin in extraretinal and retinal photoreceptors was investigated in various teleosts from different habitats and with different life strategies. UV-sensitive pineal photoreceptors were only found in the halibut, but UV-sensitive cones were identified in the retina of halibut, salmon, zebrafish,turbot and three cichlid species. In conclusion, a general developmental pattern can be outlined insofar that extraretinal photoreceptors differentiate earlier than retinal photoreceptors. An earlier differentiation of extraretinal photosensory structures in species with demersal eggs, as compared to those with pelagic embryonic life, is suggested to reflect (1) an early adaptation to the photic environment encountered by the eggs and larvae, and (2) the general developmental programme of the CNS.
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| 13. |
- Forsell, Johan, 1980-, et al.
(författare)
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Editorial: Navigating in a Measurable Epistemic Landscape
- 2020
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Ingår i: Confero. - : Linköping University Electronic Press. - 2001-4562. ; 7:1, s. 5-9
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- For this special issue on Navigating in a Measurable Epistemic Landscape we invited contributions from scholars with various disciplinary backgrounds to debate the measurable epistemic values, logics and practices of educational institutions such as school and university. Hence, we further the discussion of Confero’s first issue Managing by Measuring: Academic Knowledge Production under the Ranks (Nylander et al., 2013) by highlighting the measurable epistemic landscape of the broader educational system.
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| 14. |
- Forsell, Johan, 1980-, et al.
(författare)
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Group Work Assessment : Assessing Social Skills at Group Level
- 2020
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Ingår i: Small Group Research. - : SAGE Publications. - 1046-4964 .- 1552-8278. ; 51:1, s. 87-124
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Tidskriftsartikel (refereegranskat)abstract
- Group work assessment is often described by teachers as complex and challenging, with individual assessment and fair assessment emerging as dilemmas. The aim of this literature review is to explore and systematize research about group work assessment in educational settings. This is an integrated research area consisting of research combining group work and classroom assessment. A database search was conducted, inspired by the guidelines of the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses). The analysis and categorization evolved into a typology consisting of five themes: (a) purpose of group work assessment, (b) what is assessed in group work, (c) methods for group work assessment, (d) effects and consequences of group work assessment, and (e) quality in group work assessment. The findings reveal that research in the field of group work assessment notably focuses on social skills and group processes. Peer assessment plays a prominent role and teachers as assessors are surprising absences in the reviewed research.
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| 15. |
- Forsell, Johan, 1980-
(författare)
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Grupparbetsbedömning på individnivå : Gymnasielärares möjligheter och utmaningar
- 2022
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Avhandlingens syfte är att öka kunskapen om och förståelsen av gymnasielärares möjligheter och utmaningar med att genomföra grupparbetsbedömning på individnivå av elevers kunskaper och förmågor utvecklade i samarbete. Fokus är på om och i så fall hur lärares perspektiv förändras i samband med genomförandet av en grupparbetsuppgift i klassrummet som inkluderar lärares grupparbetsbedömning på individnivå. Syftet med avhandlingen besvaras genom en kvalitativ forskningsansats. Avhandlingen omfattar fyra empiriska delstudier. Den första delstudien, en systematisk litteraturgenomgång omfattade 83 publikationer. Den andra och tredje delstudien baserades på 12 intervjuer med sex gymnasielärare. Den fjärde delstudien, baserad på 24 videoobservationer av sex gymnasielärare. Avhandlingens resultat visar att tidigare forskning om grupparbetsbedömning har fokuserat på att särskilja vem som bidragit med vad vid grupparbete. Fokus har legat på elevers sociala förmågor och grupprocesser snarare än bedömning av elevers kunskaper och förmågor. Utmaningar lärare beskriver med grupparbetsbedömning är att kunna särskilja enskilda prestationer från gruppens. Att kunna särskilja elevers individuella kunskaper och förmågor hänger också samman med att kunna skaffa ett underlag för grupparbetsbedömning, vilket är avgörande för att få både validitet och reliabilitet i bedömningen. En specifik utmaning med grupparbetsbedömning är att tillgodose dess tudelade syfte att både kunna genomföra bedömningar av god kvalitet och samtidigt stödja gruppers samarbete. Genom att använda en metod för individuell grupparbetsbedömning med muntliga tvärgruppspresentationer kan lärare samla underlag för elevers individuella kunskaper och förmågor och samtidigt stötta gruppers samarbete vid grupparbete. Vid muntliga tvärgruppspresentationer är interaktion mellan lärare och elever och mellan elever viktigt för att kunna möjliggöra ett underlag för bedömning av elevers individuella kunskaper och förmågor. För att interaktion ska uppstå krävs det att läraren tar en aktiv roll vid tvärgruppspresentationer och stimulerar med frågor och reflektioner.
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| 16. |
- Forsell, Johan, 1980-
(författare)
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Lärobok i kommunikation
- 2013. - 1
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- Lärobok i kommunikation är en nyskriven bok för 100p kursen i Kommunikation, tänkt för studieförberedandeprogram.Att kommunicera är något djupt mänskligt. Hela tiden sänder människor ut signaler med sitt kroppsspråk, sin röst eller kanske genom medier. I denna bok i kommunikation tittar vi närmare på vad kommunikation egentligen är.Författaren presenterar kommunikationsteorier men också en del handfasta och konkreta råd i hur man blir en bättre kommunikatör. I boken betonas särskilt delar som kommunikation i konflikter, hur det sociala sammanhanget påverkar vårt sätt att kommunicera och framväxten av mediesamhället och mediekulturer. Den löpande framställningen varvas med case, övningsuppgifter och intressanta fördjupningsrutor.Lärobok i kommunikation passar dig som:Vill få både teoretiska modeller och handfasta tips.Ha en bok framtagen för kursplanen i Gy 2011.Vill läsa en bok med ett tydligt berättande språk.
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| 17. |
- Forsell, Johan, 1980-
(författare)
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Lärobok i sociologi
- 2012. - 1
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- Lärobok i sociologi vänder sig till elever som läser 100p sociologi på studieförberedande gymnasieprogram.Boken är en introduktion till ämnet skriven direkt för gymnasiet. Läsaren presenteras för klassikerna, de grundläggande metodfrågorna, teorier och modeller. Centrala begrep som individ, identitet, genus, klass och etnicitet får också stort utrymme i presentationen.Tre kapitel har större metodavsnitt om sociologisk metod. Sist i varje kapitel finns en sammanfattning, uppgifter och tips på litteratur för vidare studier.Lärobok i sociologi är:en nyskriven boken bok anpassad för gymnasieten bok med ett berättande språk
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| 18. |
- Forsell, Johan, 1980-
(författare)
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Läromedel i sociologi
- 2017. - 2
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Bok (övrigt vetenskapligt/konstnärligt)
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| 19. |
- Forsell, Johan, et al.
(författare)
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Molecular identification and developmental expression of UV and green opsin mRNAs in the pineal organ of the Atlantic halibut.
- 2002
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Ingår i: Developmental Brain Research. - 0165-3806. ; 136:1, s. 51-62
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Tidskriftsartikel (refereegranskat)abstract
- The pineal organ is the only differentiated photoreceptor organ present in embryos and early larvae of the Atlantic halibut (Hippoglossus hippoglossus). We investigated the molecular identity of opsins in the pineal organ, and their expression during different life stages. Using RT-PCR we identified two 681-bp gene sequences, named HPO1 and HPO4, in cDNA from adult pineal and whole embryos. The predicted amino acid sequences showed highest identity to the transmembrane regions of teleostean RH2 green cone opsins (HPO1, 72-91%) and SWS-1 UV cone opsins (HPO4, 71-83%). In situ hybridization revealed expression of HPO1 and HPO4 mRNA transcripts in photoreceptors in the pineal organ of embryos, larvae and adults. HPO1 and HPO4 mRNA transcripts were also expressed in the larval retina. Our study provides molecular evidence for short and middle wavelength light sensitive photoreceptors in the pineal organ of Atlantic halibut throughout life, and suggests that pineal photoreception may play an important role during embryonic and larval life stages, especially at the time when the retina does not possesses corresponding photoreceptor capacity.
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| 20. |
- Forsell, Johan, et al.
(författare)
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Role of the pineal organ in the photoregulated hatching of the Atlantic halibut
- 1997
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Ingår i: International Journal of Developmental Biology. - 0214-6282. ; 41:4, s. 591-595
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Tidskriftsartikel (refereegranskat)abstract
- The timing of hatching in the Atlantic halibut (Hippoglossus hippoglossus) has been suggested to be regulated by environmental light conditions. However, the photosensory organ that perceives the triggering light has not been identified. In the present study, we investigated the morphogenesis of the pineal organ and the neurochemical differentiation of photoreceptors in the pineal organ and the retina of the Atlantic halibut during embryonic development. Immunocytochemical techniques were used for detection of integral protein components of the phototransduction process: opsins, arrestin (S-antigen) and α-transducin. We also studied the expression of serotonin (5-HT), a precursor of the neurohormone melatonin known to be synthesized by pineal photoreceptors. In the pineal anlage, opsin immunoreactive (ir) cells appear at 11 days post-fertilization (pf), arrestin, α-transducin and serotonin ir cells appear at 14 days pf; hatching took place 15 days pf. The retina contained no immunoreactive cells in embryos or in newly hatched larva. During this period, the pineal anlage is morphologically discernible only as a wedge-shaped region in the diencephalic roof, where elongated cells are aligned with their long axes converging toward a centrally located presumptive pineal lumen. The results show that the pineal photoreceptors contain serotonin and molecules involved in the phototransduction cascade before hatching. We suggest that the pineal organ has the capacity to perceive and mediate photic information before hatching in halibut embryos, and may thereby influence the timing of hatching.
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| 21. |
- Forsell, Johan, 1980-, et al.
(författare)
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Teachers’ perceived challenges in group work assessment
- 2021
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Ingår i: Cogent Education. - : Taylor & Francis. - 2331-186X. ; 8:1, s. -16
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Tidskriftsartikel (refereegranskat)abstract
- Group work assessment is a challenging and complex practice for teachers. This study focuses on the challenges teachers perceive before and after participating in a group work assessment project that emphasizes individual assessment. By conducting a qualitative thematic analysis of twelve interviews with six teachers at upper secondary schools in Sweden, several challenges could be identified. The most prominent challenge concerning group work assessment is how to discern students’ individual performance within groups. This challenge has consequences for both the validity and the fairness of the assessment. Further, teachers experienced challenges with (un)fairness in group work assessment, in terms of both achieving fairness and having to deal with students’ emotions regarding perceived unfairness. The results also show how teachers perceive inadequate conditions, such as a lack of time and methods, and generate challenges in their practice, which is also related to reliability.
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| 22. |
- Forsell, Johan, 1980-, et al.
(författare)
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The “Bashing” of Education Research
- 2018
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Ingår i: Confero. - : Linköping University Electronic Press. - 2001-4562. ; 6:1, s. 5-12
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- This special issue of Confero presents six essays with different aims and scope, which relate to the criticism or “bashing” of educational research. Starting with Martin Malmström’s essay How do you think it feels? On Being the Epitome of Pseudoscience that clearly connects to the bashing theme, the other essays elucidate and question related aspects of the universities, of pedagogy, teacher education and educational research. Together the essays form a dialogue on the underpinning perspectives on science and learning, not only in the field on education but academia at large. To clarify, the intention of this issue is not to constrain the critics, but an ambition to deepen the conversation and open up for different perspectives and voices.
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| 23. |
- Forsell, Joakim, et al.
(författare)
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The relation between Blastocystis and the intestinal microbiota in Swedish travellers
- 2017
-
Ingår i: BMC Microbiology. - : Springer Science and Business Media LLC. - 1471-2180. ; 17
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Tidskriftsartikel (refereegranskat)abstract
- Background: Blastocystis sp. is a unicellular eukaryote that is commonly found in the human intestine. Its ability to cause disease is debated and a subject for ongoing research. In this study, faecal samples from 35 Swedish university students were examined through shotgun metagenomics before and after travel to the Indian peninsula or Central Africa. We aimed at assessing the impact of travel on Blastocystis carriage and seek associations between Blastocystis and the bacterial microbiota.Results: We found a prevalence of Blastocystis of 16/35 (46%) before travel and 15/35 (43%) after travel. The two most commonly Blastocystis subtypes (STs) found were ST3 and ST4, accounting for 20 of the 31 samples positive for Blastocystis. No mixed subtype carriage was detected. All ten individuals with a typable ST before and after travel maintained their initial ST. The composition of the gut bacterial community was not significantly different between Blastocystis-carriers and non-carriers. Interestingly, the presence of Blastocystis was accompanied with higher abundances of the bacterial genera Sporolactobacillus and Candidatus Carsonella. Blastocystis carriage was positively associated with high bacterial genus richness, and negatively correlated to the Bacteroides-driven enterotype. These associations were both largely dependent on ST4 – a subtype commonly described from Europe – while the globally prevalent ST3 did not show such significant relationships.Conclusions: The high rate of Blastocystis subtype persistence found during travel indicates that long-term carriage of Blastocystis is common. The associations between Blastocystis and the bacterial microbiota found in this study could imply a link between Blastocystis and a healthy microbiota as well as with diets high in vegetables. Whether the associations between Blastocystis and the microbiota are resulting from the presence of Blastocystis, or are a prerequisite for colonization with Blastocystis, are interesting questions for further studies.
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| 24. |
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| 25. |
- Furukawa, Toshi A., et al.
(författare)
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Dismantling, optimising, and personalising internet cognitive behavioural therapy for depression : a systematic review and component network meta-analysis using individual data
- 2021
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Ingår i: Lancet psychiatry. - London, United Kingdom : Elsevier. - 2215-0374 .- 2215-0366. ; 8:6, s. 500-511
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Forskningsöversikt (refereegranskat)abstract
- Findings We identified 76 RCTs, including 48 trials contributing individual participant data (11 704 participants) and 28 trials with aggregate data (6474 participants). The participants' weighted mean age was 42.0 years and 12 406 (71%) of 17 521 reported were women. There was suggestive evidence that behavioural activation might be beneficial (iMD -1.83 [95% credible interval (CrI) -2.90 to -0.80]) and that relaxation might be harmful (1.20 [95% CrI 0.17 to 2.27]). Baseline severity emerged as the strongest prognostic factor for endpoint depression. Combining human and automated encouragement reduced dropouts from treatment (incremental odds ratio, 0.32 [95% CrI 0.13 to 0.93]). The risk of bias was low for the randomisation process, missing outcome data, or selection of reported results in most of the included studies, uncertain for deviation from intended interventions, and high for measurement of outcomes. There was moderate to high heterogeneity among the studies and their components. 511
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| 26. |
- Giron, Maria Stella T, et al.
(författare)
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Psychotropic drug use in elderly people with and without dementia
- 2001
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Ingår i: International Journal of Geriatric Psychiatry. - : Wiley. - 0885-6230 .- 1099-1166. ; 16:9, s. 900-906
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Tidskriftsartikel (refereegranskat)abstract
- ObjectiveTo determine the prevalence of psychotropic drug use in very old persons with and without dementia in two time periods, and describe the patterns of psychotropic drug use between institutions and non-institutions.MethodsDescriptive analysis on a sample of subjects aged 81+ from a population-based study in Stockholm, Sweden. Psychotropic drug use data were collected from the 1987-1989 and 1994-1996 periods of the study. The diagnosis of dementia was based on the DSM III-R.ResultsAbout 41% of the subjects used at least one psychotropic drug in both periods. Women and subjects in institutions more commonly used psychotropic drugs. The most commonly reported were, in rank order, hypnotics-sedatives, anxiolytics, antipsychotics and antidepressants. Hypnotics-sedatives and anxiolytics were the most commonly used in both institutions and non-institutions. More persons with dementia used psychotropic drugs in both periods. The use of newer drugs, for example, SSRI, was evident. Multivariate analyses showed increased risk for psychotropic drug use among subjects in institutions.ConclusionsThis study confirms the high rate of psychotropic drug use in the very old, particularly in persons with dementia. Psychotropic drug use was high among subjects living in institutions.
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| 27. |
- Giron, Maria Stella T, et al.
(författare)
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Sleep problems in a very old population : drug use and clinical correlates
- 2002
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Ingår i: The journals of gerontology. Series A, Biological sciences and medical sciences. - 1079-5006 .- 1758-535X. ; 57:4, s. M236-40
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Tidskriftsartikel (refereegranskat)abstract
- Background. Complaints of disturbed or dissatisfied sleep are common among older people. This study aimed to evaluate the prevalence of sleep problems in very old persons and its relation to physical and mental health and drug use. Methods. This is a cross-sectional analysis of sleep problems in a population of old persons living in Stockholm, Sweden. There were 641 subjects aged 81+ years; 77.8% were women, 91.4% were noninstitutionalized, and 68.6% lived alone. All persons underwent a comprehensive medical and psychiatric interview and examination. Sleep problems were assessed using the Clinical Psychopathological Rating Scale (CPRS). Covariates included chronic medical conditions, depression, dementia, pain, self-rated health, activities of daily living, use of hypnotics-sedatives, use of other psychotropic drugs, and use of nonpsychotropic drugs. Results. More than one third of subjects were identified with sleep problems. They were more common among women and persons using a higher number of drugs. Poor self-rated health, depression, and pain were related to the presence of sleep problems. Among persons with sleep problems and depression, only 19.2% used antidepressants, and 46.2% used hypnotics-sedatives. Among persons with sleep problems and pain, 63.2% used analgesics, and 47.0% used hypnotics-sedatives. One or more chronic diseases, use of hypnotics-sedatives, use of other psychotropic drugs, and use of nonpsychotropic drugs were also related to sleep problems. After multivariate analysis, factors significantly related to sleep problems were female gender, depression, pain, and hypnotic-sedative use. Conclusions. Sleep problems were common in this very old population. These results suggest the importance of carefully assessing an older person's complaints to accurately diagnose and effectively treat sleep problems.
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| 28. |
- Granvik, Christoffer, et al.
(författare)
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Olfactory dysfunction as an early predictor for post-COVID condition at 1-year follow-up
- 2024
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Ingår i: Brain and Behavior. - : John Wiley & Sons. - 2162-3279. ; 14:6
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Tidskriftsartikel (refereegranskat)abstract
- Background: Olfactory dysfunction together with neurological and cognitive symptoms are common after COVID-19. We aimed to study whether performance on olfactory and neuropsychological tests following infection predict post-COVID condition (PCC), persisting symptoms, and reduced health-related quality of life.Methods: Both hospitalized (N = 10) and non-hospitalized individuals (N = 56) were enrolled in this prospective cohort study. Participants were evaluated 1–3 months after infection with an olfactory threshold test and neuropsychological tests, which was used as predictors of PCC. A questionnaire outlining persisting symptoms and the validated instrument EuroQol five-dimension five-level for health-related quality of life assessment were used as outcome data 1 year after infection (N = 59). Principal component analysis was used to identify relevant predictors for PCC at 1 year.Results: Objectively assessed olfactory dysfunction at 1–3 months post infection, but not subjective olfactory symptoms, predicted post-COVID condition with reduced health-related quality of life (PCC+) at 1 year. The PCC+ group scored more often below the cut off for mild cognitive impairment on the Montreal Cognitive Assessment (61.5% vs. 21.7%) and higher on the Multidimensional Fatigue Inventory-20, compared to the group without PCC+.Conclusion: Our results indicate that objectively assessed, olfactory dysfunction is a predictor for PCC+. These findings underscore the importance of objective olfactory testing. We propose that olfactory screening in the early post-acute phase of COVID-19 infection might identify individuals that are at higher risk of developing long-term health sequalae.
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| 29. |
- Gröning, Remigius, et al.
(författare)
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Immune response to SARS-CoV-2 mRNA vaccination in multiple sclerosis patients after rituximab treatment interruption
- 2023
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Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 14
-
Tidskriftsartikel (refereegranskat)abstract
- Peripheral B cell depletion via anti-CD20 treatment is a highly effective disease-modifying treatment for reducing new relapses in multiple sclerosis (MS) patients. A drawback of rituximab (RTX) and other anti-CD20 antibodies is a poor immune response to vaccination. While this can be mitigated by treatment interruption of at least six months prior to vaccination, the timing to resume treatment while maintaining subsequent vaccine responses remains undetermined. Here, we characterized SARS-CoV-2 S-directed antibody and B cell responses throughout three BNT162b2 mRNA vaccine doses in RTX-treated MS patients, with the first two doses given during treatment interruption. We examined B-cell mediated immune responses in blood samples from patients with RTX-treated MS throughout three BNT162b2 vaccine doses, compared to an age- and sex-matched healthy control group. The first vaccine dose was given 1.3 years (median) after the last RTX infusion, the second dose one month after the first, and the third dose four weeks after treatment re-initiation. We analyzed SARS-CoV-2 S-directed antibody levels using enzyme-linked immunosorbent assay (ELISA), and the neutralization capacity of patient serum against SARS-CoV-2 S-pseudotyped lentivirus using luciferase reporter assay. In addition, we assessed switched memory (CD19+CD20+CD27+IgD-), unswitched memory (CD19+CD20+CD27+IgD+), naïve (CD19+CD20+CD27-IgD+), and double negative (DN, CD19+CD20+CD27-IgD-) B cell frequencies, as well as their SARS-CoV-2 S-specific (CoV+) and Decay Accelerating Factor-negative (DAF-) subpopulations, using flow cytometry. After two vaccine doses, S-binding antibody levels and neutralization capacity in SARS-CoV-2-naïve MS patients were comparable to vaccinated healthy controls, albeit with greater variation. Higher antibody response levels and CoV+-DN B cell frequencies after the second vaccine dose were predictive of a boost effect after the third dose, even after re-initiation of rituximab treatment. MS patients also exhibited lower frequencies of DAF- memory B cells, a suggested proxy for germinal centre activity, than control individuals. S-binding antibody levels in RTX-treated MS patients after two vaccine doses could help determine which individuals would need to move up their next vaccine booster dose or postpone their next RTX infusion. Our findings also offer first indications on the potential importance of antigenic stimulation of DN B cells and long-term impairment of germinal centre activity in rituximab-treated MS patients.
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| 30. |
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| 31. |
- Gustafsson, Fredrik, et al.
(författare)
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Particle Filters for Positioning, Navigation and Tracking
- 2002
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Ingår i: IEEE Transactions on Signal Processing. - : Institute of Electrical and Electronics Engineers (IEEE). - 1053-587X .- 1941-0476. ; 50:2, s. 425-437
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Tidskriftsartikel (refereegranskat)abstract
- A framework for positioning, navigation and tracking problems using particle filters (sequential Monte Carlo methods) is developed. It consists of a class of motion models and a general non-linear measurement equation in position. A general algorithm is presented, which is parsimonious with the particle dimension. It is based on marginalization, enabling a Kalman filter to estimate all position derivatives, and the particle filter becomes low-dimensional. This is of utmost importance for high-performance real-time applications. Automotive and airborne applications illustrate numerically the advantage over classical Kalman filter based algorithms. Here the use of non-linear models and non-Gaussian noise is the main explanation for the improvement in accuracy. More specifically, we describe how the technique of map matching is used to match an aircraft's elevation profile to a digital elevation map, and a car's horizontal driven path to a street map. In both cases, real-time implementations are available, and tests have shown that the accuracy in both cases is comparable to satellite navigation (as GPS), but with higher integrity. Based on simulations, we also argue how the particle filter can be used for positioning based on cellular phone measurements, for integrated navigation in aircraft, and for target tracking in aircraft and cars. Finally, the particle filter enables a promising solution to the combined task of navigation and tracking, with possible application to airborne hunting and collision avoidance systems in cars.
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| 32. |
- Hellgren, Fredrika, et al.
(författare)
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Modulation of innate immune response to mRNA vaccination after SARS-CoV-2 infection or sequential vaccination in humans
- 2024
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Ingår i: JCI Insight. - : American Society for Clinical Investigation (ASCI). - 2379-3708. ; 9:9
-
Tidskriftsartikel (refereegranskat)abstract
- mRNA vaccines are likely to become widely used for the prevention of infectious diseases in the future. Nevertheless, a notable gap exists in mechanistic data, particularly concerning the potential effects of sequential mRNA immunization or preexisting immunity on the early innate immune response triggered by vaccination. In this study, healthy adults, with or without documented prior SARS-CoV-2 infection, were vaccinated with the BNT162b2/Comirnaty mRNA vaccine. Prior infection conferred significantly stronger induction of proinflammatory and type I IFN-related gene signatures, serum cytokines, and monocyte expansion after the prime vaccination. The response to the second vaccination further increased the magnitude of the early innate response in both study groups. The third vaccination did not further increase vaccine-induced inflammation. In vitro stimulation of PBMCs with TLR ligands showed no difference in cytokine responses between groups, or before or after prime vaccination, indicating absence of a trained immunity effect. We observed that levels of preexisting antigen-specific CD4 T cells, antibody, and memory B cells correlated with elements of the early innate response to the first vaccination. Our data thereby indicate that preexisting memory formed by infection may augment the innate immune activation induced by mRNA vaccines.
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| 33. |
- Hellgren, Johan, 1966-, et al.
(författare)
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Bias of feedback cancellation algorithms in hearing aids based on direct closed loop identification
- 2001
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Ingår i: IEEE transactions on speech and audio processing. - : Institute of Electrical and Electronics Engineers (IEEE). - 1063-6676 .- 1558-2353. ; 9:8, s. 906-913
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Tidskriftsartikel (refereegranskat)abstract
- The undesired effects of acoustic feedback of hearing aids can be reduced with an internal feedback path that is an estimate of the external feedback path. This paper analyzes the limiting estimate of the feedback for feedback cancellation schemes that apply some recursive prediction error method with a quadratic norm, e.g., least mean square (LMS) and recursive least squares (RLS), to the output and input signals of the hearing aid to identify the feedback path. The data used for identification is then collected in closed loop and the estimate used in one recursion will affect the data used in succeeding recursions. These properties have to be considered in the analysis. The analysis shows that the limiting estimate may be biased if there is an error in the used model of the input signal to the hearing aid, and that the system is not identifiable unless a second input signal to the system is added to the output of the hearing aid or the signal processing of the hearing aid used to modify the signal to the impaired ear is nonlinear. The limiting estimate is presented as the solution to an optimization problem in the frequency domain. An analytical expression of the limiting estimate is presented for a special case. For other cases an algorithm is presented that can be used to find a numerical solution. The results can be useful when the model structure used with the recursive identification is chosen.
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| 34. |
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| 35. |
- Holmqvist, Bo, et al.
(författare)
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The early ontogeny of neuronal nitric oxide synthase systems in the zebrafish.
- 2004
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Ingår i: Journal of Experimental Biology. - : The Company of Biologists. - 1477-9145 .- 0022-0949. ; 207:Pt 6, s. 923-935
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Tidskriftsartikel (refereegranskat)abstract
- To examine a putative role for neuronal nitric oxide synthase (nNOS) in early vertebrate development we investigated nNOS mRNA expression and cGMP production during development of the zebrafish Danio rerio. The nNOS mRNA expression in the central nervous system (CNS) and periphery showed a distinct spatio-temporal pattern in developing zebrafish embryo and young larvae. nNOS mRNA expression was first detected at 19 h postfertilisation (h.p.f.), in a bilateral subpopulation of the embryonic ventrorostral cell cluster in the forebrain. The number of nNOS mRNA-expressing cells in the brain slowly increased, also appearing in the ventrocaudal cell cluster from about 26 h.p.f., and in the dorsorostral and hindbrain cell cluster and in the medulla at 30 h.p.f. A major increase in nNOS mRNA expression started at about 40 h.p.f., and by 55 h.p.f. the expression constituted cell populations in differentiated central nuclei and in association with the proliferation zones of the brain, and in the medulla and retina. In parts of the skin, nNOS mRNA expression started at 20 h.p.f. and ended at 55 h.p.f. Between 40 and 55 h.p.f., nNOS mRNA expression started in peripheral organs, forming distinct populations after hatching within or in the vicinity of the presumptive swim bladder, enteric ganglia, and along the alimentary tract and nephritic ducts. Expression of nNOS mRNA correlated with the neuronal differentiation pattern and with the timing and degree of cGMP production. These studies indicate spatio-temporal actions by NO during embryogenesis in the formation of the central and peripheral nervous system, with possible involvement in processes such as neurogenesis, organogenesis and early physiology.
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| 36. |
- Isfoss, Björn Logi, et al.
(författare)
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Stellate cells and mesenchymal stem cells in benign mammary stroma are associated with risk factors for breast cancer - an observational study
- 2018
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Ingår i: BMC Cancer. - : Springer Science and Business Media LLC. - 1471-2407. ; 18:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: It is not known whether stromal cells in benign breast tissue can mediate risk of breast cancer. We recently described aldehyde dehydrogenase 1 A1 (ALDH1) positive (+) cells in morphologically normal breast stroma of premenopausal women, and the data indicated that their distribution is associated with clinical risk factors for breast cancer. The aim of the present study was to define the identities of these cells using histologic and immunohistologic methods, and to investigate associations between those cells and hormonal and genetic risk factors in pre- and postmenopausal women. Methods: Stroma of morphologically normal tissue was analyzed in samples from 101 well-characterized women whose breasts had been operated. Morphology and immunolabeling were applied to determine cell identities based on the putative stem cell markers ALDH1 and stage-specific embryonic antigen-3 (SSEA3), and immunophenotypes indicating mast cells or stellate cells. The results were compared with the patients' risk factors using regression analysis (two-tailed). Results: ALDH1+ round/oval cells were associated with low parity in BRCA1/2 carriers (p=0.022), while in non-BRCA1/2-carriers they were negatively associated with nulliparity (p=0.057). In premenopausal women ALDH1+ round/oval cells were associated with family history (p=0.058). SSEA3+ round/oval cells were morphologically and immunohistologically consistent with multilineage stress-enduring (Muse) cells, and these cells were independently associated with the breast cancer risk factors low parity (p=0.015), family history (p=0.021), and hormone use after menopause (p=0.032). ALDH1+ spindle-shaped/polygonal cells were immunohistologically consistent with stellate cells, and were negatively associated with family history of breast cancer (p=0.001). Conclusion: This study identified novel stromal cell types in benign breast tissue that have a potential for stratifying women for breast cancer risk.
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| 37. |
- Kaku, Chengzi I., et al.
(författare)
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Broad anti–SARS-CoV-2 antibody immunity induced by heterologous ChAdOx1/mRNA-1273 vaccination
- 2022
-
Ingår i: Science. - : American Association for the Advancement of Science. - 0036-8075 .- 1095-9203. ; 375:6584, s. 1041-1047
-
Tidskriftsartikel (refereegranskat)abstract
- Heterologous prime-boost immunization strategies have the potential to augment COVID-19 vaccine efficacy We longitudinally profiled severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S)–specific serological and memory B cell (MBC) responses in individuals who received either homologous (ChAdOx1: ChAdOx1) or heterologous (ChAdOx1:mRNA-1273) prime-boost vaccination. Heterologous messenger RNA (mRNA) booster immunization induced higher serum neutralizing antibody and MBC responses against SARS-CoV-2 variants of concern (VOCs) compared with that of homologous ChAdOx1 boosting. Specificity mapping of circulating B cells revealed that mRNA-1273 boost immunofocused ChAdOx1-primed responses onto epitopes expressed on prefusion-stabilized S. Monoclonal antibodies isolated from mRNA-1273–booste participants displayed overall higher binding affinities and increased breadth of reactivity against VOCs relativ to those isolated from ChAdOx1-boosted individuals. Overall, the results provide molecular insight into the enhanced quality of the B cell response induced after heterologous mRNA booster vaccination.
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| 38. |
- Kaku, Chengzi I., et al.
(författare)
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Recall of preexisting cross-reactive B cell memory after Omicron BA.1 breakthrough infection
- 2022
-
Ingår i: Science immunology. - : American Association for the Advancement of Science. - 2470-9468. ; 7:73
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Tidskriftsartikel (refereegranskat)abstract
- Understanding immune responses after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) breakthrough infection will facilitate the development of next-generation vaccines. Here, we profiled spike (S)-specific B cell responses after Omicron/BA.1 infection in messenger RNA-vaccinated donors. The acute antibody response was characterized by high levels of somatic hypermutation and a bias toward recognition of ancestral SARS-CoV-2 strains, suggesting the early activation of vaccine-induced memory B cells. BA.1 breakthrough infection induced a shift in B cell immunodominance hierarchy from the S2 subunit, which is highly conserved across SARS-CoV-2 variants of concern (VOCs), and toward the antigenically variable receptor binding domain (RBD). A large proportion of RBD-directed neutralizing antibodies isolated from BA.1 breakthrough infection donors displayed convergent sequence features and broadly recognized SARS-CoV-2 VOCs. Together, these findings provide insights into the role of preexisting immunity in shaping the B cell response to heterologous SARS-CoV-2 variant exposure.
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| 39. |
- Maleki, Kimia T., et al.
(författare)
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MAIT cell activation is associated with disease severity markers in acute hantavirus infection
- 2021
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Ingår i: Cell Reports Medicine. - : Saunders Elsevier. - 2666-3791. ; 2:3
-
Tidskriftsartikel (refereegranskat)abstract
- Hantaviruses are zoonotic RNA viruses that cause severe acute disease in humans. Infected individuals have strong inflammatory responses that likely cause immunopathology. Here, we studied the response of mucosal-associated invariant T (MAIT) cells in peripheral blood of individuals with hemorrhagic fever with renal syndrome (HFRS) caused by Puumala orthohantavirus, a hantavirus endemic in Europe. We show that MAIT cell levels decrease in the blood during HFRS and that residual MAIT cells are highly activated. This activation correlates with HFRS severity markers. In vitro activation of MAIT cells by hantavirus-exposed antigen-presenting cells is dependent on type I interferons (IFNs) and independent of interleukin-18 (IL-18). These findings highlight the role of type I IFNs in virus-driven MAIT cell activation and suggest a potential role of MAIT cells in the disease pathogenesis of viral infections.
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| 40. |
- Mattsson, Maria, et al.
(författare)
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Background factors as determinants of satisfaction with care Background factors as determinants of satisfaction with care among first-episode psychosis patients.
- 2005
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Ingår i: Soc Psychiatry Psychiatry Epidemiology:1–6. ; 1-6:DOI 10.1007, s. s00127-005
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To evaluate the impact of demographic and psychosocial background factors and psychiatric and functional status before and at admission on the patients' satisfaction with care (PSC) among, first-episode psychosis (FEP) patients. Method: One year after entering the Parachute Project, 134 FEP patients completed a patient satisfaction questionnaire. Results Twenty-nine per cent of the variance of PSC was explained by factors such as educational level, social network, duration of untreated psychosis (DUP) and Global Assessment of Functioning (GAF) the year prior to onset. Conclusion: Affecting the public knowledge in psychiatric problems and psychiatric treatment, together with early intervention strategies aiming to decrease the prodromal and DUP period among FEP patients, can positively influence the pa:ients' experience of given care. By increasing the cnowledge of available psychiatric treatment, the sense of powerlessness might decrease among the affected persons, and the possibility of early help seeking might increase.
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| 41. |
- Melin, Åsa
(författare)
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Olika vägar till enhetlig skola? : En studie av grundskolans etablering på kommunal nivå, 1950-1968
- 2022
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The importance of the local community for the development of the school system during the 19th century has been touched upon by several researchers. However, interest in the changes to the school system in Sweden during the 1950s and 1960s has largely focused on developments at the national level. The picture has mostly been that the state, the formulating arena, set the rules and the municipalities, the realization arena, implemented nationally decided welfare policy. Here the interest is different; by focusing on municipal conditions and local interests, the implementation of political reforms, specifically the nine-year compulsory school, can be nuanced.By a case study comparing two municipalities in the county of Värmland, Storfors and Arvika, with different local conditions and traditions of education, this study aims to investigate and analyse the establishment of the nine-year compulsory school at the local level, thereby making local interests and the importance of local conditions visible for the establishment of the compulsory school.The processes in the two municipalities were affected by the state formulation arena's decisions but, as previous research has shown, there was a high degree of variation in how the school system was shaped locally. The same applies to local decision-making. It too was influenced and shaped based on local conditions. From the analysis of the process in Storfors and Arvika, three main results can be discerned: 1) the establishment of the primary school at the local level was largely about initiating and completing processes sideways, within and between municipalities, not only vertical processes between state and municipality. 2) The local tradition, which can also be called the local culture or the social mechanisms, influenced the processes in the two municipalities, and 3) the process in the local realization arena was largely driven by what we would call civil servants.
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| 42. |
- Nordström, Eva-Maria, et al.
(författare)
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Impacts of global climate change mitigation scenarios on forests and harvesting in Sweden
- 2016
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Ingår i: Canadian Journal of Forest Research. - : Canadian Science Publishing. - 0045-5067 .- 1208-6037. ; 46:12, s. 1427-1438
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Tidskriftsartikel (refereegranskat)abstract
- Under climate change, the importance of biomass resources is likely to increase and new approaches are needed to analyze future material and energy use of biomass globally and locally. Using Sweden as an example, we present an approach that combines global and national land-use and forest models to analyze impacts of climate change mitigation ambitions on forest management and harvesting in a specific country. National forest impact analyses in Sweden have traditionally focused on supply potential with little reference to international market developments. In this study, we use the global greenhouse gas concentration scenarios from the Intergovernmental Panel for Climate Change to estimate global biomass demand and assess potential implications on harvesting and biodiversity in Sweden. The results show that the short-term demand for wood is close to the full harvesting potential in Sweden in all scenarios. Under high bioenergy demand, harvest levels are projected to stay high over a longer time and particularly impact the harvest levels of pulpwood. The area of old forest in the managed landscape may decrease. This study highlights the importance of global scenarios when discussing national-level analysis and pinpoints trade-offs that policy making in Sweden may need to tackle in the near future.
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| 43. |
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| 44. |
- Rolf, Hannes, 1980-
(författare)
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En fackförening för hemmen : Kollektiv mobilisering, hyresgästorganisering och maktkamp på hyresmarknaden i Stockholm och Göteborg 1875–1942
- 2020
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis is a study of the collective mobilisation and organisation of tenants in Gothenburg and Stockholm between 1875 and 1942. Of special interest are the power relations and the power struggle between the landlords and the organised tenants in the same period. The similarities and differences between the tenants’ movement in Gothenburg and Stockholm played an important role in the historical process and both cities thus need to be studied and compared to each other. The concept of contentious repertoire, developed mainly in the works of Charles Tilly and Sidney Tarrow, is used to explain the methods employed by the tenants in their collective mobilisation. Other important factors considered in the thesis are the opportunity structures available and the periods of international radicalisation where the rent struggle also seems to have intensified worldwide. Both the Swedish organised landlords and tenants modelled their organisations after labour market organisation and both parties came to understand their relation as part of a class struggle. A concept borrowed from Klas Åmark, exchangeability, will be used to illustrate an important factor – the harder it was for a landlord to replace a tenant with another tenant, the better the tenants’ position. The tenants’ unions knew this and tried with militant means as well as with advocacy for tenants’ rights and increased housing construction to make it harder for the landlords to replace their tenants. Episodes of tenant militancy were frequent, in Gothenburg especially between 1923 and 1937 and in Stockholm especially between 1928 and 1936. The collective mobilisation and organisation of the tenants did alter the power relations between landlords and tenants, which can be seen both in the concessions made by landlords in numerous conflicts and in the fact that the landlords altered their organisations to defend themselves against the tenant offensive. By the end of the period, centralised collective bargaining had been largely implemented. Other strategies aimed at reducing the exchangeability were also used by the tenants. Tenant housing cooperative enterprises, first seen as a form of protest action and as an alternative to privately owned housing, eventually took on more centralised form in the organisation HSB. The close ties between HSB and the tenants’ unions gave the latter some economical backing and the former some additional legitimacy. By the end of the research period, the idea of large-scale municipal housing had taken over the role the idea of cooperative housing once had, and even though HSB was to play an important part in the post-war housing projects it would be reduced to a secondary position. When it comes to new legislation, the rent law of 1939 did little to alter the power relations,even though it did recognise the tenants’ movement as the natural representative for the tenants’ cause. The 1942 rent act, however, did give the tenants some leverage but it also overrode the system of collective bargaining that had been worked out by the tenants and landlords. All in all, the directactions, the housing production and the new legislation reduced the exchangeability of the tenants and altered the power relations in favour of the tenants.
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| 45. |
- Schagatay, Felix, et al.
(författare)
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Serum concentration of extracellular cold-inducible RNA-binding protein is associated with respiratory failure in COVID-19
- 2022
-
Ingår i: Frontiers in Immunology. - : Frontiers Media S.A.. - 1664-3224. ; 13
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Tidskriftsartikel (refereegranskat)abstract
- Uncontrolled release of damage-associated molecular patterns (DAMPs) is suggested to be a major trigger for the dysregulated host immune response that leads to severe COVID-19. Cold-inducible RNA-binding protein (CIRP), is a newly identified DAMP that aggravates inflammation and tissue injury, and induces respiratory failure in sepsis. Whether CIRP contributes to the pathogenesis of respiratory failure in COVID-19 has not yet been explored.Aim: To investigate if the concentration of extracellular CIRP (eCIRP) in serum associates with respiratory failure and lung involvement by chest computed tomography (CT) in COVID-19.Methods: Herein we report a prospective observational study of patients with COVID-19 included at two University Hospitals in Sweden between April 2020 and May 2021. Serum from hospitalized patients in Örebro (N=97) were used to assess the association between eCIRP and the level of respiratory support and its correlation with pulmonary involvement on chest CT and inflammatory biomarkers. A cohort of hospitalized and non-hospitalized patients from Umeå (N=78) was used as an external validation cohort. The severity of disease was defined according to the highest degree of respiratory support; mild disease (no oxygen), non-severe hypoxemia (conventional oxygen or high-flow nasal oxygen, HFNO <50% FiO2), and severe hypoxemia (HFNO ≥50% FiO2, mechanical ventilation). Unadjusted and adjusted linear regression was used to evaluate peak eCIRP day 0-4 in respect to severity, age, sex, Charlson comorbidity score, symptom duration, and BMI.Results: Peak eCIRP concentrations were higher in patients with severe hypoxemia and were independently associated with the degree of respiratory support in both cohorts (Örebro; p=0.01, Umeå; p<0.01). The degree of pulmonary involvement measured by CT correlated with eCIRP, rs=0.30, p<0.01 (n=97).Conclusion: High serum levels of eCIRP are associated with acute respiratory failure in COVID-19. Experimental studies are needed to determine if treatments targeting eCIRP reduces the risk of acute respiratory failure in COVID-19.
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| 46. |
- Tuiskunen-Bäck, Anne, et al.
(författare)
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Clinical and genomic characterisation of a fatal Puumala orthohantavirus case with low levels of neutralising antibodies
- 2022
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Ingår i: Infectious Diseases. - : Informa UK Limited. - 2374-4235 .- 2374-4243. ; 54:10, s. 766-772
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Orthohantaviruses are rodent-borne emerging viruses that cause haemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantavirus pulmonary syndrome in America. Transmission between humans have been reported and the case-fatality rate ranges from 0.4% to 40% depending on virus strain. There is no specific and efficient treatment for patients with severe HFRS. Here, we characterised a fatal case of HFRS and sequenced the causing Puumala orthohantavirus (PUUV).METHODS: PUUV RNA and virus specific neutralising antibodies were quantified in plasma samples from the fatal case and other patients with non-fatal PUUV infection. To investigate if the causing PUUV strain was different from previously known strains, Sanger sequencing was performed directly from the patient's plasma. Biopsies obtained from autopsy were stained for immunohistochemistry.RESULTS: The patient had approximately tenfold lower levels of PUUV neutralising antibodies and twice higher viral load than was normally seen for patients with less severe PUUV infection. We could demonstrate unique mutations in the S and M segments of the virus that could have had an impact on the severity of infection. Due to the severe course of infection, the patient was treated with the bradykinin receptor inhibitor icatibant to reduce bradykinin-mediated vessel permeability and maintain vascular circulation.CONCLUSIONS: Our data suggest that bradykinin receptor inhibitor may not be highly efficient to treat patients that are at an advanced stage of HFRS. Low neutralising antibodies and high viral load at admission to the hospital were associated with the fatal outcome and may be useful for future predictions of disease outcome.
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| 47. |
- Vikström, Linnea, et al.
(författare)
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Vaccine-induced correlate of protection against fatal COVID-19 in older and frail adults during waves of neutralization-resistant variants of concern : an observational study
- 2023
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Ingår i: The Lancet Regional Health. - : Elsevier. - 2666-7762. ; 30
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: To inform future preventive measures including repeated vaccinations, we have searched for a clinically useful immune correlate of protection against fatal COVID-19 among nursing homes residents.METHODS: We performed repeated capillary blood sampling with analysis of S-binding IgG in an open cohort of nursing home residents in Sweden. We analyzed immunological and registry data from 16 September 2021 to 31 August 2022 with follow-up of deaths to 30 September 2022. The study period included implementation of the 3rd and 4th mRNA monovalent vaccine doses and Omicron virus waves.FINDINGS: A total of 3012 nursing home residents with median age 86 were enrolled. The 3rd mRNA dose elicited a 99-fold relative increase of S-binding IgG in blood and corresponding increase of neutralizing antibodies. The 4th mRNA vaccine dose boosted levels 3.8-fold. Half-life of S-binding IgG was 72 days. A total 528 residents acquired their first SARS-CoV-2 infection after the 3rd or the 4th vaccine dose and the associated 30-day mortality was 9.1%. We found no indication that levels of vaccine-induced antibodies protected against infection with Omicron VOCs. In contrast, the risk of death was inversely correlated to levels of S-directed IgG below the 20th percentile. The death risk plateaued at population average above the lower 35th percentile of S-binding IgG.INTERPRETATION: In the absence of neutralizing antibodies that protect from infection, quantification of S-binding IgG post vaccination may be useful to identify the most vulnerable for fatal COVID-19 among the oldest and frailest. This information is of importance for future strategies to protect vulnerable populations against neutralization resistant variants of concern.FUNDING: Swedish Research Council, SciLifeLab via Knut and Alice Wallenberg Foundation, VINNOVA. Swedish Healthcare Regions, and Erling Persson Foundation.
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| 48. |
- Wessbo, Simon, 1980-, et al.
(författare)
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Open issue
- 2017
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Ingår i: Confero. - Linköping : Linköping University Electronic Press. - 2001-4562. ; 5:1, s. 5-10
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- This open issue of Confero presents four essays that all relate to questions of education and power. Laila Nielsen and Ralph Leighton compare how conditions of citizenship regarding ethnicity, gender, and social class are understood, based on interviews with teachers and students in upper secondary schools in England and Sweden. The second essay is written by Rasoul Nejadmehr who gives a thorough account of the "scientific education" as the dominant educational paradigm of the present. Through a historical analysis, Nejadmehr shows how this paradigm is deeply embedded with racial, colonial, and Eurocentric biases. The third essay by Marcus Samuelsson analyzes offical and unofficial inspections of the classroom that takes place when authorities conduct audits, but also when pupils post videos on social media. In the fourth essay of this issue, Tomas Wedin discusses changes in the Swedish school during the period of 1946-2000.
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| 49. |
- Wigren, Julia, et al.
(författare)
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At-home sampling to meet geographical challenges for serological assessment of SARS-CoV-2 exposure in a rural region of northern Sweden, March to May 2021 : a retrospective cohort study
- 2023
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Ingår i: Eurosurveillance. - : European Centre for Disease Control and Prevention (ECDC). - 1025-496X .- 1560-7917. ; 28:13
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Tidskriftsartikel (refereegranskat)abstract
- Background: The current SARS-CoV-2 pandemic has highlighted a need for easy and safe blood sampling in combination with accurate serological methodology. Venipuncture for testing is usually performed by trained staff at healthcare centres. Long travel distances to healthcare centres in rural regions may introduce a bias of testing towards relatively large communities with closer access. Rural regions are therefore often not represented in population-based data.Aim: The aim of this retrospective cohort study was to develop and implement a strategy for at-home testing in a rural region of Sweden during spring 2021, and to evaluate its role to provide equal health care for its inhabitants.Methods: We developed a sensitive method to measure antibodies to the S-protein of SARS-CoV-2 and optimised this assay for clinical use together with a strategy of at-home capillary blood sampling.Results: We demonstrated that our ELISA gave comparable results after analysis of capillary blood or serum from SARS-CoV-2-experienced individuals. We demonstrated stability of the assay under conditions that reflected temperature and humidity during winter or summer. By assessment of capillary blood samples from 4,122 individuals, we could show both feasibility of the strategy and that implementation shifted the geographical spread of testing in favour of rural areas.Conclusion: Implementation of at-home sampling enabled citizens living in remote rural areas access to centralised and sensitive laboratory antibody tests. The strategy for testing used here could therefore enable disease control authorities to get rapid access to information concerning immunity to infectious diseases, even across vast geographical distance.
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