| 1. |
- Boamah, David, et al.
(författare)
-
Peptidoglycan deacetylation controls type IV secretion and the intracellular survival of the bacterial pathogen Legionella pneumophila
- 2023
-
Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences (PNAS). - 0027-8424 .- 1091-6490. ; 120:23
-
Tidskriftsartikel (refereegranskat)abstract
- Peptidoglycan is a critical component of the bacteria cell envelope. Remodeling of the peptidoglycan is required for numerous essential cellular processes and has been linked to bacterial pathogenesis. Peptidoglycan deacetylases that remove the acetyl group of the N-acetylglucosamine (NAG) subunit protect bacterial pathogens from immune recognition and digestive enzymes secreted at the site of infection. However, the full extent of this modification on bacterial physiology and pathogenesis is not known. Here, we identify a polysaccharide deacetylase of the intracellular bacterial pathogen Legionella pneumophila and define a two-tiered role for this enzyme in Legionella pathogenesis. First, NAG deacetylation is important for the proper localization and function of the Type IVb secretion system, linking peptidoglycan editing to the modulation of host cellular processes through the action of secreted virulence factors. As a consequence, the Legionella vacuole mis-traffics along the endocytic pathway to the lysosome, preventing the formation of a replication permissive compartment. Second, within the lysosome, the inability to deacetylate the peptidoglycan renders the bacteria more sensitive to lysozyme-mediated degradation, resulting in increased bacterial death. Thus, the ability to deacetylate NAG is important for bacteria to persist within host cells and in turn, Legionella virulence. Collectively, these results expand the function of peptidoglycan deacetylases in bacteria, linking peptidoglycan editing, Type IV secretion, and the intracellular fate of a bacterial pathogen.
|
|
| 2. |
- Gilmore, Sarah, et al.
(författare)
-
Writing differently
- 2019
-
Ingår i: Management Learning. - : SAGE PUBLICATIONS LTD. - 1350-5076 .- 1461-7307. ; 50:1, s. 3-10
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- This special issue of Management Learning on 'Writing Differently' builds on a groundswell of resistance to 'scientific' norms of academic writing. These norms are restrictive, inhibit the development of knowledge and excise much of what it is to be human from our learning, teaching and research. Contributors to the special issue explore how, released from these restrictions, it is possible to touch vulnerable flesh and invoke new political and ethical practices. Through changing our norms of writing, we explore different modes of learning and change how and what we teach. By bringing the previously excised vast hinterlands of life and lives to the fore, we create the intellectual space to engender new ideas as well as more collaborative forms of learning. In so doing, we foster alternative conversations as to how we might constitute new, highly ethical and humanitarian organisations.
|
|
