| 1. |
- Glaeser, Stefanie P., et al.
(författare)
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Contrasting Effects of Singlet Oxygen and Hydrogen Peroxide on Bacterial Community Composition in a Humic Lake
- 2014
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 9:3, s. e92518-
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Tidskriftsartikel (refereegranskat)abstract
- Light excitation of humic matter generates reactive oxygen species (ROS) in surface waters of aquatic ecosystems. Abundant ROS generated in humic matter rich lakes include singlet oxygen (O-1(2)) and hydrogen peroxide (H2O2). Because these ROS differ in half-life time and toxicity, we compared their effects on microbial activity (C-14-Leucine incorporation) and bacterial community composition (BCC) in surface waters of humic Lake Grosse Fuchskuhle (North-eastern Germany). For this purpose, experiments with water samples collected from the lake were conducted in July 2006, September 2008 and August 2009. Artificially increased O-1(2) and H2O2 concentrations inhibited microbial activity in water samples to a similar extent, but the effect of the respective ROS on BCC varied strongly. BCC analysis by 16S rRNA gene clone libraries and RT-PCR DGGE revealed ROS specific changes in relative abundance and activity of major bacterial groups and composition of dominating phylotypes. These changes were consistent in the three experiments performed in different years. The relative abundance of Polynucleobacter necessarius, Limnohabitans-related phylotypes (Betaproteobacteria), and Novosphingobium acidiphilum (Alphaproteobacteria) increased or was not affected by photo-sensitized O-1(2) exposure, but decreased after H2O2 exposure. The opposite pattern was found for Actinobacteria of the freshwater AcI-B cluster which were highly sensitive to O-1(2) but not to H2O2 exposure. Furthermore, group-specific RT-PCR DGGE analysis revealed that particle-attached P. necessarius and Limnohabitans-related phylotypes exhibit higher resistance to O-1(2) exposure compared to free-living populations. These results imply that O-1(2) acts as a factor in niche separation of closely affiliated Polynucleobacter and Limnohabitans-related phylotypes. Consequently, oxidative stress caused by photochemical ROS generation should be regarded as an environmental variable determining abundance, activity, and phylotype composition of environmentally relevant bacterial groups, in particular in illuminated and humic matter rich waters.
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| 2. |
- Kämpfer, Peter, et al.
(författare)
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Proposal of Coetzeea brasiliensis gen. nov. sp. nov. isolated from larvae of Anopheles darlingi
- 2016
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Ingår i: International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society. - 1466-5026 .- 1466-5034. ; 66, s. 5211-5217
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Tidskriftsartikel (refereegranskat)abstract
- A Gram-stain-negative, rod-shaped strain, Braz8T, isolated from larvae ofAnopheles darlingiwas investigatedusing a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strainBraz8Twas related most closely to species of the genusThorsellia, with 95.6, 96.5 and 96.6% similarity to thetype strains ofThorsellia anophelis,Thorsellia kandunguensisandThorsellia kenyensis, respectively, andformed a separate branch in the phylogenetic tree next to the monophyletic cluster of the genusThorsellia.Chemotaxonomic data supported the allocation of the strain to the familyThorselliaceae. The major fatty acidswere C18:1!7c, C16:0and C14:0. The quinone system was composed of ubiquinones Q-8 and Q-7 (1:0.3),the predominant polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the polyamine patternshowed the major compound putrescine. However, qualitative and quantitative differences in the majorpolyamine, polar lipid profile and fatty acid patterns distinguished strain Braz8Tfrom species of the genusThorsellia. Phylogenetic analysis based on 16S rRNA gene sequences, average nucleotide identity, DNA–DNAhybridization, multilocus sequence analysis as well as physiological and biochemical tests distinguished strainBraz8Tboth genotypically and phenotypically from the threeThorselliaspecies but also showed its placementin the familyThorselliaceae. Thus, strain Braz8Tis considered to represent a novel species of a new genus mostclosely related to the genusThorsellia, for which the nameCoetzeea brasiliensisgen. nov., sp. nov. is proposed.The type strain ofCoetzeea brasiliensisis Braz8T(=LMG 29552T=CIP 111088T)
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| 3. |
- Kaempfer, Peter, et al.
(författare)
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Elizabethkingia anophelis sp nov., isolated from the midgut of the mosquito Anopheles gambiae
- 2011
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Ingår i: International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society. - 1466-5026 .- 1466-5034. ; 61, s. 2670-2675
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Tidskriftsartikel (refereegranskat)abstract
- The taxonomic position, growth characteristics and antibiotic resistance properties of a slightly yellow-pigmented bacterial strain, designated R26(T), isolated from the midgut of the mosquito Anopheles gambiae, were studied. The isolate produced rod-shaped cells, which stained Gram-negative. The bacterium had two growth optima at 30-31 degrees C and 37 degrees C. Strain R26(T) demonstrated natural antibiotic resistance to ampicillin, chloramphenicol, kanamycin, streptomycin and tetracycline. 16S rRNA gene sequence analysis revealed that the isolate showed 98.6% sequence similarity to that of Elizabethkingia meningoseptica ATCC 13253(T) and 98.2 % similarity to that of Elizabethkingia miricola GTC 862(T). The major fatty acids of strain R26(T) were iso-C(15:0), iso-C(17:0) 3-OH and summed feature 4 (iso-C(15:0) 2-OH and/or C(16:1)omega 7c/t. Strain R26(T) contained only menaquinone MK-6 and showed a complex polar lipid profile consisting of diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and unknown polar lipids and glycolipids. DNA-DNA hybridization experiments with E. meningoseptica CCUG 214(T) (=ATCC 13253(T)) and E. miricola KCTC 12492(T) (=GTC 862(T)) gave relatedness values of 34.5 % (reciprocal 41.5 %) and 35.0 % (reciprocal 25.7 %), respectively. DNA-DNA hybridization results and some differentiating biochemical properties indicate that strain R26T represents a novel species, for which the name Elizabethkingia anophelis sp. nov. is proposed. The type strain is R26(T) (=CCUG 60038(T) =CCM 7804(T)).
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| 4. |
- Kämpfer, Peter, et al.
(författare)
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Proposal of Thorsellia kenyensis sp. nov. and Thorsellia kandunguensis sp. nov., isolated from the larvae of Anopheles arabiensis as members of the family Thorselliaceae fam. nov.
- 2015
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Ingår i: International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society. - 1466-5026 .- 1466-5034. ; 65, s. 444-451
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Tidskriftsartikel (refereegranskat)abstract
- Two Gram-negative, rod-shaped strains, T2.1T and W5.1.1T, isolated from larvae of the mosquito Anopheles arabiensis, were investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity studies, strains T2.1T and W5.1.1T were shown to belong to the genus Thorsellia, both showing 97.8 % similarity to the type strain of Thorsellia anophelis, with 98.1 % similarity to each other. Chemotaxonomic data supported the allocation of the strains to the genus Thorsellia: their major fatty acids were C18 : 1ω7c, C16 : 0 and C14 : 0 and they harboured a ubiquinone Q-8 quinone system and a polyamine pattern with the major compound 1,3-diaminopropane. Qualitative and quantitative differences in their polar lipid profiles distinguished strains T2.1T and W5.1.1T from each other and from T. anophelis. Average nucleotide identity (ANI), DNA–DNA hybridization, multilocus sequence analysis (MLSA) as well as physiological and biochemical tests allowed T2.1T and W5.1.1T to be distinguished both genotypically and phenotypically from each other and from the type strain of T. anophelis. Thus, we propose that these isolates represent two novel species of the genus Thorsellia, named Thorsellia kenyensis sp. nov. (type strain T2.1T = CCM 8545T = LMG 28483T = CIP 110829T) and Thorsellia kandunguensis sp. nov. (type strain W5.1.1T = LMG 28213T = CIP 110794T). Furthermore, phylogenetic analysis based on nearly full-length 16S rRNA gene sequences showed that the genus Thorsellia forms a separate branch, distinct from the families Enterobacteriaceae, Pasteurellaceae and Orbaceae. As a consequence, a new family Thorselliaceae fam. nov. is proposed. An emended description of Thorsellia anophelis is also provided.
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| 5. |
- Orthová, Ivana, et al.
(författare)
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Massilia norwichensis sp. nov., isolated from an air sample
- 2015
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Ingår i: International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society. - 1466-5026 .- 1466-5034. ; 65:1, s. 56-64
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Tidskriftsartikel (refereegranskat)abstract
- A Gram-negative, rod-shaped and motile bacterial isolate, designated strain NS9T, isolated from air of the Sainsbury Centre for Visual Arts in Norwich, UK, was subjected to a polyphasic taxonomic study including phylogenetic analyses based on partial 16S rRNA, gyrB and lepA gene sequences and phenotypic characterization. The 16S rRNA gene sequence of NS9T identified Massilia haematophila CCUG 38318T, M. niastensis 5516S-1T (both 97.7 % similarity), M. aerilata 5516S-11T (97.4 %) and M. tieshanensis TS3T (97.4 %) as the next closest relatives. In partial gyrB and lepA sequences, NS9T shared the highest similarities with M. haematophila CCUG 38318T (94.5 %) and M. aerilata 5516-11T (94.3 %), respectively. These sequence data demonstrate the affiliation of NS9T to the genus Massilia. The detection of the predominant ubiquinone Q-8, a polar lipid profile consisting of the major compounds diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol and a polyamine pattern containing 2-hydroxyputrescine and putrescine were in agreement with the assignment of strain NS9T to the genus Massilia. Major fatty acids were summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0, C18 : 1ω7c and C10 : 0 3-OH. Dissimilarities in partial lepA and gyrB gene sequences as well as results from DNA–DNA hybridizations demonstrate that strain NS9T is a representative of an as-yet undescribed species of the genus Massilia that is also distinguished from its close relatives based on physiological and biochemical traits. Hence, we describe a novel species, for which we propose the name Massilia norwichensis sp. nov., with the type strain NS9T ( = CCUG 65457T = LMG 28164T).
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| 6. |
- Sanchez-Castro, Ivan, et al.
(författare)
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Stenotrophomonas bentonitica sp nov., isolated from bentonite formations
- 2017
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Ingår i: International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society. - 1466-5026 .- 1466-5034. ; 67:8, s. 2779-2786
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Tidskriftsartikel (refereegranskat)abstract
- A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7(T), was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern Spain. Comparative 16S rRNA gene sequence analysis showed that BII-R7T represented a member of the genus Stenotrophomonas (class Gammaproteobacteria), and was related most closely to Stenotrophomonas rhizophila e-p10(T) (99.2% sequence similarity), followed by Stenotrophomonas pavanii ICB 89(T) (98.5 %), Stenotrophomonas maltophilia IAM 12423(T), Stenotrophomonas chelatiphaga LPM-5(T) and Stenotrophomonas tumulicola T5916-2-1b(T) (all 98.3 %). Pairwise sequence similarities to all other type strains of species of the genus Stenotrophomonas were below 98 %. Genome- based calculations (orthologous average nucleotide identity, original average nucleotide identity, genome-to-genome distance and DNA G+C percentage) indicated clearly that the isolate represents a novel species within this genus. Different phenotypic analyses, such as the detection of a quinone system composed of the major compound ubiquinone Q-8 and a fatty acid profile with iso-C-15:0 and anteiso-C-15:0 as major components, supported this finding at the same time as contributing to a comprehensive characterization of BII-R7(T). Based on this polyphasic approach comprising phenotypic and genotypic/molecular characterization, BII-R7(T) can be differentiated clearly from its phylogenetic neighbours, establishing a novel species for which the name Stenotrophomonas bentonitica sp. nov.is proposed with BII-R7(T) as the type strain (= LMG 29893(T) = CECT 9180(T) = DSM 103927(T)).
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