| 1. |
|
|
| 2. |
- Klionsky, Daniel J., et al.
(författare)
-
Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
-
Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
-
Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
|
|
| 3. |
- Tobias, Deirdre K, et al.
(författare)
-
Second international consensus report on gaps and opportunities for the clinical translation of precision diabetes medicine
- 2023
-
Ingår i: Nature Medicine. - 1546-170X. ; 29:10, s. 2438-2457
-
Forskningsöversikt (refereegranskat)abstract
- Precision medicine is part of the logical evolution of contemporary evidence-based medicine that seeks to reduce errors and optimize outcomes when making medical decisions and health recommendations. Diabetes affects hundreds of millions of people worldwide, many of whom will develop life-threatening complications and die prematurely. Precision medicine can potentially address this enormous problem by accounting for heterogeneity in the etiology, clinical presentation and pathogenesis of common forms of diabetes and risks of complications. This second international consensus report on precision diabetes medicine summarizes the findings from a systematic evidence review across the key pillars of precision medicine (prevention, diagnosis, treatment, prognosis) in four recognized forms of diabetes (monogenic, gestational, type 1, type 2). These reviews address key questions about the translation of precision medicine research into practice. Although not complete, owing to the vast literature on this topic, they revealed opportunities for the immediate or near-term clinical implementation of precision diabetes medicine; furthermore, we expose important gaps in knowledge, focusing on the need to obtain new clinically relevant evidence. Gaps include the need for common standards for clinical readiness, including consideration of cost-effectiveness, health equity, predictive accuracy, liability and accessibility. Key milestones are outlined for the broad clinical implementation of precision diabetes medicine.
|
|
| 4. |
- Battaglia, Manuela, et al.
(författare)
-
Introducing the Endotype Concept to Address the Challenge of Disease Heterogeneity in Type 1 Diabetes
- 2020
-
Ingår i: Diabetes Care. - : American Diabetes Association. - 1935-5548 .- 0149-5992. ; 43:1, s. 5-12
-
Tidskriftsartikel (refereegranskat)abstract
- The clinical diagnosis of new-onset type 1 diabetes has, for many years, been considered relatively straightforward. Recently, however, there is increasing awareness that within this single clinical phenotype exists considerable heterogeneity: disease onset spans the complete age range; genetic susceptibility is complex; rates of progression differ markedly, as does insulin secretory capacity; and complication rates, glycemic control, and therapeutic intervention efficacy vary widely. Mechanistic and immunopathological studies typically show considerable patchiness across subjects, undermining conclusions regarding disease pathways. Without better understanding, type 1 diabetes heterogeneity represents a major barrier both to deciphering pathogenesis and to the translational effort of designing, conducting, and interpreting clinical trials of disease-modifying agents. This realization comes during a period of unprecedented change in clinical medicine, with increasing emphasis on greater individualization and precision. For complex disorders such as type 1 diabetes, the option of maintaining the "single disease" approach appears untenable, as does the notion of individualizing each single patient's care, obliging us to conceptualize type 1 diabetes less in terms of phenotypes (observable characteristics) and more in terms of disease endotypes (underlying biological mechanisms). Here, we provide our view on an approach to dissect heterogeneity in type 1 diabetes. Using lessons from other diseases and the data gathered to date, we aim to delineate a roadmap through which the field can incorporate the endotype concept into laboratory and clinical practice. We predict that such an effort will accelerate the implementation of precision medicine and has the potential for impact on our approach to translational research, trial design, and clinical management.
|
|
| 5. |
|
|
| 6. |
- Gottlieb, Laura
(författare)
-
Designing Tools for Joint Inquiry : Making and thinking together
- 2020
-
Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Positioned within the research program and subject area of Innovation and Design, this research focuses on the relationship between tools and joint inquiry. Joint inquiry – the collaborative exploration and definition of problems and possible solutions – plays an important part in Participatory Design processes. Tools, usually involving the making or using of artefacts in workshops, are developed and used to support joint inquiry between different actors. The aim of this research is to contribute to the deliberate design and systematic evaluation of tools for joint inquiry. This work addresses the literature gap comprising a lack of systematic evaluations in Participatory Design research and a need to evaluate designed activities and environments in relation to emerging designer-participant collaborations.This research is a starting point towards developing a systematic approach for designing tools for joint inquiry and introduces two frameworks for this purpose. The first is the Communities of Inquiry framework, from the field of computer-mediated communication in distance education, which is used to identify indicators in joint inquiry. The second framework is a categorisation used to distinguish different aspects of a tool. Combining the two frameworks aids the understanding of the relationships between tools and indicators for joint inquiry. A Research through Design and Research for Design approach is used to study conversations between people and in interaction with tools. Nine design experiments are described, all of which involve the design and testing of tools to support the initiation of joint inquiry. The tools were primarily created and tested within an academic context with design students and researchers, with a focus on the topic of co-production – that is, close collaboration between academia and external actors. One tool was created and used within a museum with youth and researchers.The results from the design experiments show that the tools supported the Communities of Inquiry indicators in the following ways: eliciting metaphors stimulated humour and encouraged contributions, renegotiating artefacts provoked brainstorming, commensality promoted phatic communication and humour, the relationship between material properties and metaphors prompted problem recognition, and formats directed towards personal experiences led to self-disclosure and emotional expression. The tools were a hinderance to the Communities of Inquiry framework when there was dominant participation and when the tools were considered to be inappropriate for certain work contexts. Future research will continue to develop means of systematically evaluating and designing tools that support communicative practices in Participatory Design processes.
|
|
| 7. |
- Gottlieb, Laura
(författare)
-
Exploratory workshop using abstract collaging to reflect on university-society collaborations
- 2018
-
Ingår i: Participatory Innovation Conference 2018 PIN-C 2018. ; , s. 303-309
-
Konferensbidrag (refereegranskat)abstract
- Collaborating with society (businesses, industry and municipalities) is a common practice for many universities today. To support such collaborations, there is a need to share and evaluate these practices. This research explores the use of creative and visual methods from Participatory Design to support reflective practice in university-society collaborations. The paper describes an exploratory workshop consisting of an abstract collage-making exercise and a list of questions. The purpose of this workshop is to see how the visual exercise could support discussions about university- society collaborations and to develop future studies. The results from the workshop show that using abstract collages generated multiple interpretations and metaphors about the visualized collaborations. The collage-making exercise prompted discussions about the interrelationship between collaboration, innovation processes and knowledge creation. It also stimulated playful interactions between participants. Moreover, the workshop participants gave valuable feedback on how to develop materials and questions to discuss university- society collaborations.
|
|
| 8. |
|
|
| 9. |
- Gottlieb, Laura
(författare)
-
Relational Sensitivity and Participatory Practice : Exploring Poise and Punctuation through an Empirical Study
- 2022
-
Ingår i: ACM International Conference Proceeding Series. - New York, NY, USA : Association for Computing Machinery. - 9781450396813 ; , s. 186-190
-
Konferensbidrag (refereegranskat)abstract
- Poise-self-awareness of how one is and acts, and punctuation-attunement to relational dynamics, describe integral relational sensitivities for practitioners in Participatory Design (PD). These concepts describe important personal and embodied "practices of readiness"for participating in contingent and relational processes of designing with others. However, more attention on these "practices of readiness"is required in design research and design training to better comprehend and develop relevant relational sensitivity and competences. Through an empirical study, this paper explores what relational sensitivity i.e., poise and punctuation, could be in a participatory practice and what it can do for the process. The paper also explores ways in which relational sensitivity could be nurtured through participatory practices. This research contributes to PD literature by connecting concepts related to relational sensitivity with an empirical study, elaborating on the role of relational sensitivity in PD practice.
|
|
| 10. |
- Gottlieb, Laura
(författare)
-
Relational Sensitivity in Participatory Design : Thinking and making together through joint inquiry
- 2023
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Participatory design processes can entail involving individuals, groups, and communities in joint inquiry to deliberate on their current situation, imagine alternative futures, and forge possible collaborations. Relational sensitivity – drawing attention to relationships in a given situation – is important when staging (i.e., organising and designing for) and facilitating joint inquiry. It is a valuable concept with which to illuminate and discuss contingent circumstances that may arise and, more generally, to support participation in joint inquiry. This PhD thesis explores and develops views of and a vocabulary for discussing relational sensitivity so as to support practitioners in staging joint inquiry. The thesis includes both a literature review and practice-based studies, applying a research through design approach to discuss relational sensitivity. The practice-based studies examine two participatory design processes: the collaborative designing of a communal space with university students, and the development of shared practices and rituals in a co-working space with professionals from the public sector. The practice-based studies also include nine design experiments in which situations for joint inquiry have been staged in academic contexts and in a museum. Through the literature- and practice-based studies, the thesis addresses two research questions: “How can relational sensitivity be understood when staging situations for joint inquiry?” and “How can the study of relational sensitivity inform practitioners when staging situations for joint inquiry?” Research methods include the use of field notes, journal notes, collective analysis, audio- and video-recordings, and questionnaires.The results include three perspectives from which to understand relational sensitivity when staging joint inquiry: sensitivity to self (e.g., reflexivity, self-awareness, and embodied awareness), sensitivity to intersubjective dynamics (e.g., attunement and responsiveness to group dynamics, affect, emotions, values, trust, and power dynamics), and sensitivity to materiality and process (e.g., the roles of materials, artefacts, and activities in reflection, social cohesion, and diverse contexts, and the role of the body in power dynamics). While relational sensitivity is primarily discussed in relation to practitioners – i.e., those staging joint inquiry – this thesis also discusses the importance of cultivating participants’ awareness of the self and others when staging joint inquiry to collectively support participation.Overall, this research contributes to the research fields of innovation and design and of participatory design by developing knowledge of relational sensitivity and its role in staging situations for joint inquiry. It provides insights into how practitioners can develop, discuss, and study their relational sensitivity and support participants in participatory design processes.
|
|
| 11. |
- Gottlieb, Laura, et al.
(författare)
-
Students' comprehension of design collaborations with external organizations
- 2019
-
Ingår i: Proceedings of the 22nd International Conference on Engineering Design (ICED19). - : Cambridge University Press (CUP). ; , s. 579-588
-
Konferensbidrag (refereegranskat)abstract
- This pilot study examines how design students comprehend collaborations with external organizations— the roles and involvement of different actors in a design process. The study looks at two undergraduate courses where a total of 33 design students collaborate with a municipality and governmental agency. Data focuses on the students' terminology in regard to the external organizations and is collected through questionnaires, voice recording, workshops and written assignments. The data analysis is both quantitative and qualitative, focusing on the word frequency and semantics of the terminology. The results show that the students were not used to working closely to external organizations. This is reflected in the prevalent use of the term "client" instead of "collaborative partner". Not working closely with organizations nor users is reflected in the students’ inabilities to handle the complexities that emerge when working with multiple stakeholder and users. At the end of the paper, suggestions are made of ways to develop students’ comprehension of ways to involve external organizations and users in the design process.
|
|
| 12. |
- Gottlieb, Laura, et al.
(författare)
-
Teatime : Exploring ways to support diverse narratives on sustainability through design
- 2022
-
Ingår i: The Design Journal. - : ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD. - 1460-6925 .- 1756-3062. ; 25:1, s. 44-61
-
Tidskriftsartikel (refereegranskat)abstract
- In the design research community, diverse narratives and ontologies are discussed in relation to sustainability. Relational ontology is proposed as an alternative to the dominant dualist ontology as a way to reconnect people with their ecological embeddedness and responsibility. This work presents a dialogical tool called 'teatime' created to introduce diverse, immaterial perspectives on sustainability in a co-design project with youth and researchers. The study explores the role of the teatime design in eliciting diverse narratives and forming a dialogical space. The results show that the teatime supported reflections on immaterial perspectives, bringing out relational and social values related to the ecological crises. This study uses a systematic evaluation to reveal a micro-material perspective on ways in which the teatime design and facilitation supported the inquiry process. We propose that the design practitioners take on the role of crafting dialogical spaces that support social relationships and evoke immaterial perspectives.
|
|
| 13. |
- Gottlieb, Laura
(författare)
-
The Knowledge Triangle Cards : Supporting university-society collaborations
- 2017
-
Ingår i: Proceedings of the 11th European Conference on Games Based Learning, ECGBL 2017. - : Academic Conferences and Publishing International Limited. - 9781911218562 ; , s. 807-813
-
Konferensbidrag (refereegranskat)abstract
- The Knowledge Triangle Cards (KT Cards) is an educational tool designed for a pilot course at Mälardalen University which aims to help students to contextualize studies to global and national societal needs and to collaborate more closely with society (business and municipalities). The aim is to have students become better at choosing assignments, projects and thesis topics that tie in closer to the labour market and societal needs. The KT Cards are based on a traditional deck of cards and the suits represent different aspects of the knowledge context: opportunity, process, outcome, and stakeholder. Additionally, a set of gamified and non-gamified exercises were designed to prompt reflection and discussion about how student projects incorporate these aspects. A pilot study was carried out in order to test and develop the educational tools and future studies. A total of 24 students and teachers participated and data was collected through observations and feedback from participants. The study indicates that the gamified activities could influence reflection and discussion by speeding up pace of the activity and affecting group dynamics. Two aspects were identified as potentially prompting gamification-shorter time frames and having multiple groups completing the activities. Further studies will look more closely at these aspects for designing gamification and how this affects discussion. The next design iteration will implement minor changes to the visual design of the cards and review how the prototype could provide students with know-how on university-society collaborations and a deeper understanding of the Knowledge Triangle concept.
|
|
| 14. |
- Klionsky, Daniel J., et al.
(författare)
-
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
- 2008
-
Ingår i: Autophagy. - : Landes Bioscience. - 1554-8627 .- 1554-8635. ; 4:2, s. 151-175
-
Forskningsöversikt (refereegranskat)abstract
- Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
|
|
| 15. |
- Schaeffer, Jennie, PhD, 1974-, et al.
(författare)
-
Carbon Dioxide Theatre : at the museum
- 2020. - 1
-
Bok (populärvet., debatt m.m.)abstract
- This book is about the Carbon Dioxide Theatre project, in which we explored how participatory theatre and participatory design can be used in a museum to create engagement with climate issues.The Carbon Dioxide Theatre project focused on young people’s ability to reflect on and critically review social norms and practices around climate targets and carbon dioxide emissions, although the process affected all participants. Objects from the museum’s existing collections were central to the project to create reflections on past and present social norms.With this text, we want to inspire you to explore how museums can develop new ways of working with collections and communication related to our cultural heritage and contemporary global challenges.In the text, we have mixed descriptions of the process and the methods with personal stories from us in the project in order to share as many different perspectives of the project as possible with you. We hope that you will get inspired!
|
|
