| 1. |
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| 2. |
- Dupont, Chris L., et al.
(författare)
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Functional Tradeoffs Underpin Salinity-Driven Divergence in Microbial Community Composition
- 2014
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 9:2, s. e89549-
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Tidskriftsartikel (refereegranskat)abstract
- Bacterial community composition and functional potential change subtly across gradients in the surface ocean. In contrast, while there are significant phylogenetic divergences between communities from freshwater and marine habitats, the underlying mechanisms to this phylogenetic structuring yet remain unknown. We hypothesized that the functional potential of natural bacterial communities is linked to this striking divide between microbiomes. To test this hypothesis, metagenomic sequencing of microbial communities along a 1,800 km transect in the Baltic Sea area, encompassing a continuous natural salinity gradient from limnic to fully marine conditions, was explored. Multivariate statistical analyses showed that salinity is the main determinant of dramatic changes in microbial community composition, but also of large scale changes in core metabolic functions of bacteria. Strikingly, genetically and metabolically different pathways for key metabolic processes, such as respiration, biosynthesis of quinones and isoprenoids, glycolysis and osmolyte transport, were differentially abundant at high and low salinities. These shifts in functional capacities were observed at multiple taxonomic levels and within dominant bacterial phyla, while bacteria, such as SAR11, were able to adapt to the entire salinity gradient. We propose that the large differences in central metabolism required at high and low salinities dictate the striking divide between freshwater and marine microbiomes, and that the ability to inhabit different salinity regimes evolved early during bacterial phylogenetic differentiation. These findings significantly advance our understanding of microbial distributions and stress the need to incorporate salinity in future climate change models that predict increased levels of precipitation and a reduction in salinity.
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| 3. |
- Akner, Gunnar, 1953-, et al.
(författare)
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Vi står gärna bakom en utfallsbaserad vård
- 2017
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Ingår i: Dagens Samhälle. - 1652-6511.
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- Jörgen Nordenström försöker få det till att vår kritik av värdebaserad vård egentligen handlar om att vi vill ha mer resurser. Han har helt missuppfattat oss, skriver 26 specialistläkare i en replik.
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| 8. |
- Granéli, Edna, et al.
(författare)
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The ecophysiology and bloom dynamics of Prymnesium spp.
- 2012
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Ingår i: Harmful Algae. - : Elsevier BV. - 1568-9883 .- 1878-1470. ; 14:SI, s. 260-270
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Tidskriftsartikel (refereegranskat)abstract
- Members of Prymnesium belong to the division Haptophyta, class Prymnesiophyceae, order Prymnesialesand family Prymnesiaceae. As most haptophytes, members of the genus Prymnesium are unicellular andplanktonic. The most known of these species is the ichthyotoxic P. parvum, which may form nearlymonospecific dense blooms in coastal and inland waters. This species possesses extraordinary plasticityconcerning life survival strategies, and is specifically addressed in this review.Toxins produced by P. parvum have hemolytic properties, that not only kill fish but also co-existingplankton. These substances are allelopathic (when other algae are killed) and grazer deterrent (whengrazers are killed). Allelopathy enables P. parvum to utilize inorganic nutrients present in the surroundingwater without competition from other algal species; and by eliminating its grazers P. parvum reduces celllosses. The paralized microalgae and/or zooplankton, are therefter ingested by the P. parvum cells, aprocess called phagotrophy. P. parvum is also able of osmotrophy, i.e. utilization of dissolved organicmatter. In this review, the cellular characteristics, life cycles, bloom formation, and factors affectingtoxicity, allelopathy, phagotrophy, and osmotrophy of P. parvum are discussed.
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| 9. |
- Hagström, Johannes, et al.
(författare)
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Domoic acid production and elemental composition of two Pseudo-nitzschia multiseries strains, from the NW and SW Atlantic Ocean, growing in phosphorus- or nitrogen-limited chemostat cultures.
- 2011
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Ingår i: Journal of Plankton Research. - : Oxford University Press (OUP). - 0142-7873 .- 1464-3774. ; 33:2, s. 297-308
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Tidskriftsartikel (refereegranskat)abstract
- Here we compare cell physiology and domoic acid (DA) production for two strains of the diatom Pseudo-nitzschia multiseries originating from two opposite latitudes: Canada (CA) and Brazil (BR). The algae were grown as chemostat cultures at 0.2, 0.3 and 0.4 day−1 under nitrogen (N)- and phosphorus (P)-deficient conditions. The level of deficiency significantly affected the atomic C:N, C:P, C:Si and N:P ratios in both strains. In both strains, P per cell was 2–4× higher in the N- than in the P-deficient cultures. The opposite was not found for N in the P-deficient cultures, as shown by the N:P ratios and C:N ratios. The C:N and C:P ratios were significantly lower in the CA strain, and this did not change due to the level of deficiency. The concentration and production of DA per cell per day were significantly higher for both strains under P deficiency as expected since the toxin is rich in N. However, DA was also produced by both strains during continuous cell division under N deficiency. High or low bacterial densities associated with P. multiseries did not increase or decrease DA production. Our data imply that more attention needs to be given to the N:P ratios and concentrations in the waters where these algae occur, as both N and P deficiencies affect DA production and cellular DA concentrations.
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| 10. |
- Hagström, Johannes, et al.
(författare)
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Potential methods for managing Prymnesium parvum blooms and toxicity, with emphasis on clay and barley straw: A review
- 2010
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Ingår i: Journal of the American Water Resources Association. - : Wiley. - 1093-474X .- 1752-1688. ; 46:1, s. 187-198
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Tidskriftsartikel (refereegranskat)abstract
- Harmful algal bloom (HAB) control and mitigation is a complex problem in ecosystem management. Phytoplankton play an important role in aquatic ecosystems as primary producers and food sources for many commercially important shellfish and there are limited options for targeting just a single species within the community. Chemical treatments (e.g., algaecides), rotting barley straw, nitrogen and phosphorus manipulation, and clay and/or flocculants are but a few techniques tested or used to reduce fish kills or shellfish contamination during a HAB event. Prymnesium parvum control has focused on the use of chemicals, nutrient manipulation, and clay flocculation. However, many HAB control methods have been rejected due to their effects on ecosystems, high costs, or limited effects on target organisms. For example, rotting barley straw (Hordeum vulgare) is considered to be an environmentally friendly alternative, but has been found to have very different results on the phytoplankton community depending on the dominating taxa and is ineffective against P. parvum and dinoflagellate blooms. Clay flocculation is a useful control/mitigation technique during fish kills in marine aquaculture sites in South Korea and can be effective in freshwater if the correct combination of clay and flocculent is used. Toxins produced by P. parvum and Karenia brevis also bind to phosphatic clay, thereby removing and/or neutralizing the toxins, but there is concern that the clay will have a negative effect on sessile organisms. Some shellfish suffer high mortalities and significant impacts on somatic and reproductive tissue growth at high clay loads; however, benthic communities appear to be unchanged after five years of clay treatment in South Korea. There are likely site-specific and ecosystem-specific characteristics that make generalizations about control options difficult and require careful assessment of options at each location.
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| 13. |
- Johansson, Håkan, et al.
(författare)
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Utvärdering av länsstyrelsernas gemensamma delprogram för provbankning och analys av miljögifter i fisk under perioden 2013–2019
- 2020
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Haltmätningar av olika miljögifter i fisk har ibland använts som underlag för att utfärda kostrekommendationer. Upprepade haltmätningar från samma lokaler kan även användas till att utvärdera hur miljögiftsbelastningen förändras över tiden. Efter införlivandet av EU:s ramvattendirektiv i svensk lagstiftning har dessa haltmätningar fått ytterligare en uttalad funktion – att ge underlag till bedömning av olika vattens status och åtgärdsbehov gällande de kemiska ämnen som har givna gränsvärden för biota.Åldern och storleken hos insamlad fisk har varierat kraftigt inom det gemensamma delprogrammet. Fisk bäst lämpad för löpande miljögiftsanalyser tillhör inte samma ålders- och storlekskategori som den till human konsumtionvanligen använda. Detta innebär i praktiken att båda dessa syften med övervakningen är svårförenliga med användning av en gemensam, enhetlig metodik.Fisk har hittills insamlats från alltför få tillfällen för att möjliggöra statistiskt säkra trendanalyser. Provbankad fisk har hanterats enligt protokoll utfärdade av Naturhistoriska riksmuseet, vilket möjliggör framtida studier av miljögiftshalteri dessa prover. En del redan upparbetade och analyserade individer föll utanför det rekommenderade storleksintervallet enligt museets protokoll för insamling, preparation och provbankning av fisk.Resultaten från fisk i de undersökta vattenförekomsterna går delvis att använda för bedömning av kemisk ytvattenstatus för de ämnen som har gränsvärden för biota. Även i detta fall är det viktigt att protokollet följs, vilket inte alltid har gjorts inom delprogrammet. Här är det främst abborre som har undersökts, vilket är positivt med tanke på det stora referensmaterialet som finns för just den arten. I regel har åldern hos insamlad abborre varit mellan tre till sex år, men från Indalsälven var den insamlade fisken av jämförelsevis hög ålder; i intervallet 7 till 15 år. För bästa möjliga utvärdering av mätvärdena är det viktigast att känna till åldern och hälsotillståndet (konditionsfaktorn) hos denfisk som analyserats. För ämnesgrupper som inte har gränsvärden för biotaenligt Havs- och vattenmyndighetens föreskrifter (HVMFS 2019:25) undersöktes om halterna var mätbara i fiskmuskel och lever. Med undantag för kvicksilver låg metallhalterna i muskel från samtliga lokaler under rapporteringsgränsen.Metallhalterna i lever låg mestadels över rapporteringsgränsen.Troligen behövs särskilda rutiner för varje delsyfte. Beroende på syftet skiljerbland annat valet av den kroppsvävnad hos fisk som ska upparbetas och analyseras. För både kostrekommendations- och statusklassningssyften krävs helst analyser av halter i muskel, så att halterna direkt kan jämföras med av HVMFS 2019:25 givna gränsvärden för ämnen i fiskmuskel. Beroende på de kemiska ämnenas olika fördelningspreferenser är muskel inte alltid den bästa vävnaden att analysera. För vattenförvaltningens syfte kan det ibland vara lämpligt att välja annan vävnad än muskel och istället tillämpa de omvandlingsfaktorer som är framtagna för jämförelse mellan halter av ett ämne i olika vävnader. Omvandlingsberäkningarna kan däremot ha en negativ effekt på resultatets tillförlitlighet, eftersom den bakomliggande variationen i sambanden mellan halter i olika vävnader tillför en ökad statistisk mätosäkerhet att ta hänsyn till.Inom delprogrammet har vi även undersökt hur skillnader i analysresultatmellan olika laboratorier avseende ett urval av ämnen från ett och samma provkan försvåra tolkning av statusklassificering inom vattenförvaltningen. Dettaresulterar i en rekommendation om att använda medelfetthalter vid fetthaltnormalisering och att begära uppgifter från analyslaboratorierna om den använda metodiken för samtliga bearbetningar.Behovet av ett fortsatt länsstyrelsegemensamt delprogram är därför stort, framförallt med fokus på enhetlig hantering av paketering, märkning och storleksurval av fisk som skickas in för provbankning. Att kunna göra gemensamma upphandlingar mellan olika parter är en kostnadseffektiv och resurseffektiv satsning även framöver. En annan viktig samordningsfråga som kan hanteras inom delprogrammet är tolkning och implementering av analysdata för uppföljning av vattnets kemiska och ekologiska status.
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| 14. |
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| 15. |
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| 16. |
- Mahmoodi, Bakhtawar K., et al.
(författare)
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Association of Factor V Leiden With Subsequent Atherothrombotic Events A GENIUS-CHD Study of Individual Participant Data
- 2020
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Ingår i: Circulation. - : Ovid Technologies (Wolters Kluwer Health). - 0009-7322 .- 1524-4539. ; 142:6, s. 546-555
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Tidskriftsartikel (refereegranskat)abstract
- Background: Studies examining the role of factor V Leiden among patients at higher risk of atherothrombotic events, such as those with established coronary heart disease (CHD), are lacking. Given that coagulation is involved in the thrombus formation stage on atherosclerotic plaque rupture, we hypothesized that factor V Leiden may be a stronger risk factor for atherothrombotic events in patients with established CHD.Methods: We performed an individual-level meta-analysis including 25 prospective studies (18 cohorts, 3 case-cohorts, 4 randomized trials) from the GENIUS-CHD (Genetics of Subsequent Coronary Heart Disease) consortium involving patients with established CHD at baseline. Participating studies genotyped factor V Leiden status and shared risk estimates for the outcomes of interest using a centrally developed statistical code with harmonized definitions across studies. Cox proportional hazards regression models were used to obtain age- and sex-adjusted estimates. The obtained estimates were pooled using fixed-effect meta-analysis. The primary outcome was composite of myocardial infarction and CHD death. Secondary outcomes included any stroke, ischemic stroke, coronary revascularization, cardiovascular mortality, and all-cause mortality.Results: The studies included 69 681 individuals of whom 3190 (4.6%) were either heterozygous or homozygous (n=47) carriers of factor V Leiden. Median follow-up per study ranged from 1.0 to 10.6 years. A total of 20 studies with 61 147 participants and 6849 events contributed to analyses of the primary outcome. Factor V Leiden was not associated with the combined outcome of myocardial infarction and CHD death (hazard ratio, 1.03 [95% CI, 0.92-1.16];I-2=28%;P-heterogeneity=0.12). Subgroup analysis according to baseline characteristics or strata of traditional cardiovascular risk factors did not show relevant differences. Similarly, risk estimates for the secondary outcomes including stroke, coronary revascularization, cardiovascular mortality, and all-cause mortality were also close to identity.Conclusions: Factor V Leiden was not associated with increased risk of subsequent atherothrombotic events and mortality in high-risk participants with established and treated CHD. Routine assessment of factor V Leiden status is unlikely to improve atherothrombotic events risk stratification in this population.
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| 17. |
- McNamee, Sara E., et al.
(författare)
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Distribution, occurrence and biotoxin composition of the main shellfish toxin producing microalgae within European waters : A comparison of methods of analysis
- 2016
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Ingår i: Harmful Algae. - : Elsevier BV. - 1568-9883 .- 1878-1470. ; 55, s. 112-120
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Tidskriftsartikel (refereegranskat)abstract
- Harmful algal blooms (HABs) are a natural global phenomena emerging in severity and extent. Incidents have many economic, ecological and human health impacts. Monitoring and providing early warning of toxic HABs are critical for protecting public health. Current monitoring programmes include measuring the number of toxic phytoplankton cells in the water and biotoxin levels in shellfish tissue. As these efforts are demanding and labour intensive, methods which improve the efficiency are essential. This study compares the utilisation of a multitoxin surface plasmon resonance (multitoxin SPR) biosensor with enzyme-linked immunosorbent assay (ELISA) and analytical methods such as high performance liquid chromatography with fluorescence detection (HPLC-FLD) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) for toxic HAB monitoring efforts in Europe. Seawater samples (n = 256) from European waters, collected 2009-2011, were analysed for biotoxins: saxitoxin and analogues, okadaic acid and dinophysistoxins 1/2 (VDU /DTX2) and domoic acid responsible for paralytic shellfish poisoning (PSP), diarrheic shellfish poisoning (DSP) and amnesic shellfish poisoning (ASP), respectively. Biotoxins were detected mainly in samples from Spain and Ireland. France and Norway appeared to have the lowest number of toxic samples. Both the multitoxin SPR biosensor and the RNA microarray were more sensitive at detecting toxic HABs than standard light microscopy phytoplankton monitoring. Correlations between each of the detection methods were performed with the overall agreement, based on statistical 2 x 2 comparison tables, between each testing platform ranging between 32% and 74% for all three toxin families illustrating that one individual testing method may not be an ideal solution. An efficient early warning monitoring system for the detection of toxic HABs could therefore be achieved by combining both the multitoxin SPR biosensor and RNA microarray. (C) 2016 Elsevier B.V. All rights reserved.
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| 18. |
- Patel, Riyaz S., et al.
(författare)
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Association of Chromosome 9p21 With Subsequent Coronary Heart Disease Events : A GENIUS-CHD Study of Individual Participant Data
- 2019
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Ingår i: Circulation. - 2574-8300. ; 12:4
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Genetic variation at chromosome 9p21 is a recognized risk factor for coronary heart disease (CHD). However, its effect on disease progression and subsequent events is unclear, raising questions about its value for stratification of residual risk.METHODS: A variant at chromosome 9p21 (rs1333049) was tested for association with subsequent events during follow-up in 103 357 Europeans with established CHD at baseline from the GENIUS-CHD (Genetics of Subsequent Coronary Heart Disease) Consortium (73.1% male, mean age 62.9 years). The primary outcome, subsequent CHD death or myocardial infarction (CHD death/myocardial infarction), occurred in 13 040 of the 93 115 participants with available outcome data. Effect estimates were compared with case/control risk obtained from the CARDIoGRAMplusC4D consortium (Coronary Artery Disease Genome-wide Replication and Meta-analysis [CARDIoGRAM] plus The Coronary Artery Disease [C4D] Genetics) including 47 222 CHD cases and 122 264 controls free of CHD.RESULTS: Meta-analyses revealed no significant association between chromosome 9p21 and the primary outcome of CHD death/myocardial infarction among those with established CHD at baseline (GENIUSCHD odds ratio, 1.02; 95% CI, 0.99-1.05). This contrasted with a strong association in CARDIoGRAMPlusC4D odds ratio 1.20; 95% CI, 1.18-1.22; P for interaction < 0.001 compared with the GENIUS-CHD estimate. Similarly, no clear associations were identified for additional subsequent outcomes, including all-cause death, although we found a modest positive association between chromosome 9p21 and subsequent revascularization (odds ratio, 1.07; 95% CI, 1.04-1.09).CONCLUSIONS: In contrast to studies comparing individuals with CHD to disease-free controls, we found no clear association between genetic variation at chromosome 9p21 and risk of subsequent acute CHD events when all individuals had CHD at baseline. However, the association with subsequent revascularization may support the postulated mechanism of chromosome 9p21 for promoting atheroma development.
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| 19. |
- Patel, Riyaz S., et al.
(författare)
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Subsequent Event Risk in Individuals With Established Coronary Heart Disease : Design and Rationale of the GENIUS-CHD Consortium
- 2019
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Ingår i: Circulation. - 2574-8300. ; 12:4
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: The Genetics of Subsequent Coronary Heart Disease (GENIUS-CHD) consortium was established to facilitate discovery and validation of genetic variants and biomarkers for risk of subsequent CHD events, in individuals with established CHD.METHODS: The consortium currently includes 57 studies from 18 countries, recruiting 185 614 participants with either acute coronary syndrome, stable CHD, or a mixture of both at baseline. All studies collected biological samples and followed-up study participants prospectively for subsequent events.RESULTS: Enrollment into the individual studies took place between 1985 to present day with a duration of follow-up ranging from 9 months to 15 years. Within each study, participants with CHD are predominantly of self-reported European descent (38%-100%), mostly male (44%-91%) with mean ages at recruitment ranging from 40 to 75 years. Initial feasibility analyses, using a federated analysis approach, yielded expected associations between age (hazard ratio, 1.15; 95% CI, 1.14-1.16) per 5-year increase, male sex (hazard ratio, 1.17; 95% CI, 1.13-1.21) and smoking (hazard ratio, 1.43; 95% CI, 1.35-1.51) with risk of subsequent CHD death or myocardial infarction and differing associations with other individual and composite cardiovascular endpoints.CONCLUSIONS: GENIUS-CHD is a global collaboration seeking to elucidate genetic and nongenetic determinants of subsequent event risk in individuals with established CHD, to improve residual risk prediction and identify novel drug targets for secondary prevention. Initial analyses demonstrate the feasibility and reliability of a federated analysis approach. The consortium now plans to initiate and test novel hypotheses as well as supporting replication and validation analyses for other investigators.
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| 20. |
- Pérez Blanco, Eva, et al.
(författare)
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Detection of Heterosigma akashiwo (Hada) using specific RNA probes : Variability of RNA content with environmental conditions
- 2013
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Ingår i: Harmful Algae. - : Elsevier BV. - 1568-9883 .- 1878-1470. ; 24, s. 80-88
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Tidskriftsartikel (refereegranskat)abstract
- The raphidophyte Heterosigma akashiwo, which forms toxic blooms, causes major economical losses to the fish industry because of the fish kills involved. It is therefore important to be able to detect not only H. akashiwo but other toxic phytoplankton species as well, rapidly and accurately to reduce losses by fish kills. With this purpose, DNA sequences from H. akashiwo 18S and 28S rRNA gene regions were studied in silico to design species-specific probes to be used in a microarray format. Three strains of H. akashiwo (AC 265, AC 266 and GUMACC 120) were grown at optimal conditions and transferred into new environmental conditions changing either the light intensity, salinity, temperature or nutrient concentrations, to check if any of these environmental conditions induced changes in the cellular RNA concentration. The aim of this experiment was the calibration of several species-specific probes for the quantification of H. akashiwo. Differences on RNA content were not significant (p < 0.05) in any of the treatments, therefore the calibration curves were validated. The designed probes are reliable for the detection and quantification of H. akashiwo cells in natural waters. (C) 2013 Elsevier B.V. All rights reserved.
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| 21. |
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| 22. |
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| 23. |
- Tajic, Denis, 1984-
(författare)
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Mellan policy och praktik : En studie om nyanlända elevers pedagogiska och sociala inkludering i skolan
- 2022
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The overall aim of the thesis is to investigate how schools’ formal and informal structures affect the pedagogical and social inclusion of newly arrived students, as well as how newly arrived students in grades 7-9 and school staff themselves understand and handle these structures. Formal structures refer to forms of pedagogical measures for newly arrived students. Informal structures refer to opportunities for and the quality of interactions and social relations between, on the one hand, newly arrived students and, on the other, non-newly arrived students and school staff.The majority of the newly arrived students in this study came to Sweden during or immediately after the so-called refugee crisis of 2015 and 2016. This is also a period when an extensive educational policy reform was carried out in Sweden in order to provide better conditions for schools to organize the reception and education of newly arrived students.By using a critical policy analysis, the theoretical focus is placed on a critical and contextualized understanding of how policy implementation can be understood in the relation between local practices and national support measures. The thesis is based on an ethnographic approach, and the empirical material was collected in two primary schools in the Stockholm region during academic year 2018/2019. Besides fieldnotes, interviews with newly arrived students and school staff formed the basis for the analysis.The thesis involves three empirical studies. In Study I, the meaning of inclusion is constructed in relation to three contextual perspectives: the contextual requirements, contextual opportunities and contextual limits. The study shows that organizational and pedagogical "solutions", whose purpose is to include newly arrived students, sometimes have exclusionary premises. The school staff constantly find a way to legitimize their practices in relation to what the policy prescribes.Study II shows that the students and teachers can influence the school's institutional habitus through informal structures. This entails creating places where newly arrived students are accepted as a legitimate part of the school's educational and social environment, in which solidarity comes to the fore through the institutional habitus of multicultural incorporation.In Study III, the analyses show that the schools’ authorized policy actors, such as school leaders, or in some cases teachers, have a legitimate mandate to interpret and enact policy regarding multilingual classroom assistance, which sometimes turns out to be different than what was intended in a certain policy decision. However, there are some actors who do not have a legitimate mandate, but in some cases, they can influence the formal structures and adapt local policy as nonauthorized policy actors.In conclusion, the thesis shows how the school contexts can offer belonging when implementation of formal policy has already created otherness. The outcome is thus not a unilateral distribution of power by principals and teachers, but their power is reduced by the students' relational practices.
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| 24. |
- Venge, Per, et al.
(författare)
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Equal clinical performance of a novel point-of-care cardiac troponin I (cTnI) assay with a commonly used high-sensitivity cTnI assay
- 2017
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Ingår i: Clinica Chimica Acta. - : ELSEVIER SCIENCE BV. - 0009-8981 .- 1873-3492. ; 469, s. 119-125
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Tidskriftsartikel (refereegranskat)abstract
- Background: Efficient rule-out of acute myocardial infarction (MI) facilitates early disposition of chest pain patients in emergency departments (ED). Point-of-care (POC) cardiac troponin (cTn) may improve patient throughput. We compared the diagnostic accuracy of a novel cTnI test (Minicare cTnI, Philips), with current POC cTnI (I-Stat, Abbott) and high-sensitivity central laboratory cTnI (hs-cTnI; Architect, Abbott) assays.Methods: The clinical performance of the assays were compared in samples from 450 patients from a previous clinical evaluation of Minicare cTnI.Results: Minicare cTnI correlated with Architect hs-cTnI (r(2) = 0.85, p < 0.0001) and I-Stat cTnI (r(2) = 0.93, p < 0.0001). Areas under the receiver operating characteristics curves were 0.87-0.91 at admission (p = ns) and 0.96-0.97 3 h after admission (p = ns). The negative predictive values (NPV) at admission were 95% ((92-97%, 95% CI) for Minicare cTnI and increased to 99% (97-100%) at 2-4 h, and similar to Architect hs-cTnI (98%, 96-100%), but higher than I-Stat cTnI (95%, 92-97%; p < 0.01). Negative likelihood ratios (LR) after 2-4 h were 0.06 (0.02-0.17, 95% CI) for Minicare cTnI, 0.11 (0.05-0.24) for Architect hs-cTnI (p = 0.02) and 0.28 (0.18-0.43) for I-Stat cTnI (p < 0.0001). The clinical concordances between Minicare cTnI and Architect hs-cTnI were 92% (admission) and 95% (2-4 h), with lower concordances between Minicare cTnI and I-Stat cTnI (83% and 78%, respectively; p = 0.007).Conclusions: The Minicare cTnI POC assay may become useful for prompt and safe ruling-out of AMI in ED patients with suspected AMI using a guideline supported 0/3 h sampling protocol.
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| 25. |
- Wen, Su, et al.
(författare)
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Effects of rice straw on the cell viability, photosynthesis, and growth of Microcystis aeruginosa
- 2014
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Ingår i: Chinese Journal of Oceanology and Limnology. - : Springer Science and Business Media LLC. - 0254-4059 .- 1993-5005. ; 32:1, s. 120-129
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Tidskriftsartikel (refereegranskat)abstract
- Rice straw is supposed to be an environment-friendly biomaterial for inhibiting the growth of harmful blooms of the cyanobacterium Microcystis aeruginosa. However, its potential mechanism is not well known. To explore this mechanism, the growth, cell viability (esterase activity, membrane potential, and membrane integrity), photosynthesis, and cell size of M. aeruginosa were determined using flow cytometry and Phyto-PAM after exposure to rice straw extracts (RSE). The results show that doses from 2.0 to 10.0 g/L of RSE efficiently inhibited the alga for 15 days, while the physiologic and morphologic responses of the cyanobacteria were time-dependent. RSE interfered with the cell membrane potential, cell size, and in vivo chlorophyll-a fluorescence on the first day. After 7 days of exposure, RSE was transported into the cytosol, which disrupted enzyme activity and photosynthesis. The cyanobacteria then started to repair its physiology (enzyme activity, photosynthesis) and remained viable, suggesting that rice straw act as an algistatic agent.
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