| 1. |
- Hansson Wahlberg, Tobias, et al.
(författare)
-
Are there any Institutional Facts?
- 2019
-
Ingår i: Maurinian Truths : Essays in Honour of Anna-Sofia Maurin on her 50th Birthday - Essays in Honour of Anna-Sofia Maurin on her 50th Birthday. - 9789188899538 - 9789188899545 ; , s. 83-88
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)
|
|
| 2. |
- Ekenberg, Love, et al.
(författare)
-
Deliberation, representation, equity : research approaches, tools and algorithms for participatory processes
- 2017
-
Bok (övrigt vetenskapligt/konstnärligt)abstract
- What can we learn about the development of public interaction in e-democracy from a drama delivered by mobile headphones to an audience standing around a shopping center in a Stockholm suburb? In democratic societies there is widespread acknowledgment of the need to incorporate citizens' input in decision-making processes in more or less structured ways. But participatory decision making is balancing on the borders of inclusion, structure, precision and accuracy. To simply enable more participation will not yield enhanced democracy, and there is a clear need for more elaborated elicitation and decision analytical tools. This rigorous and thought-provoking volume draws on a stimulating variety of international case studies, from flood risk management in the Red River Delta of Vietnam, to the consideration of alternatives to gold mining in Ro?ia Montana in Transylvania, to the application of multi-criteria decision analysis in evaluating the impact of e-learning opportunities at Uganda's Makerere University. Editors Love Ekenberg (senior research scholar, International Institute for Applied Systems Analysis [IIASA], Laxenburg, professor of Computer and Systems Sciences, Stockholm University), Karin Hansson (artist and research fellow, Department of Computer and Systems Sciences, Stockholm University), Mats Danielson (vice president and professor of Computer and Systems Sciences, Stockholm University, affiliate researcher, IIASA) and Göran Cars (professor of Societal Planning and Environment, Royal Institute of Technology, Stockholm) draw innovative collaborations between mathematics, social science, and the arts. They develop new problem formulations and solutions, with the aim of carrying decisions from agenda setting and problem awareness through to feasible courses of action by setting objectives, alternative generation, consequence assessments, and trade-off clarifications. As a result, this book is important new reading for decision makers in government, public administration and urban planning, as well as students and researchers in the fields of participatory democracy, urban planning, social policy, communication design, participatory art, decision theory, risk analysis and computer and systems sciences.
|
|
| 3. |
- Hansson, Mattias, et al.
(författare)
-
Amino acid residues His183 and Glu264 in Bacillus subtilis ferrochelatase direct and facilitate the insertion of metal ion into protoporphyrin IX
- 2007
-
Ingår i: Biochemistry. - : American Chemical Society (ACS). - 0006-2960 .- 1520-4995. ; 46:1, s. 87-94
-
Tidskriftsartikel (refereegranskat)abstract
- Ferrochelatase catalyzes the terminal step in the heme biosynthetic pathway, i.e., the incorporation of Fe(II) into protoporphyrin IX. Various biochemical and biophysical methods have been used to probe the enzyme for metal binding residues and the location of the active site. However, the location of the metal binding site and the path of the metal into the porphyrin are still disputed. Using site-directed mutagenesis on Bacillus subtilis ferrochelatase we demonstrate that exchange of the conserved residues His183 and Glu264 affects the metal affinity of the enzyme. We also present the first X-ray crystal structure of ferrochelatase with iron. Only a single iron was found in the active site, coordinated in a square pyramidal fashion by two amino acid residues, His183 and Glu264, and three water molecules. This iron was not present in the structure of a His183Ala modified ferrochelatase. The results strongly suggest that the insertion of a metal ion into protoporphyrin IX by ferrochelatase occurs from a metal binding site represented by His183 and Glu264.
|
|
| 4. |
- Hansson, Mattias, et al.
(författare)
-
Bacterial ferrochelatase turns human: Tyr13 determines the apparent metal specificity of Bacillus subtilis ferrochelatase.
- 2011
-
Ingår i: Journal of Biological Inorganic Chemistry. - : Springer Science and Business Media LLC. - 1432-1327 .- 0949-8257. ; 16:2, s. 235-242
-
Tidskriftsartikel (refereegranskat)abstract
- Ferrochelatase catalyzes the insertion of Fe(2+) into protoporphyrin IX. The enzymatic product heme (protoheme IX) is a well-known cofactor in a wide range of proteins. The insertion of metal ions other than Fe(2+) occurs rarely in vivo, but all ferrochelatases that have been studied can insert Zn(2+) at a good rate in vitro. Co(2+), but not Cu(2+), is known to be a good substrate of the mammalian and Saccharomyces cerevisiae ferrochelatases. In contrast, Cu(2+), but not Co(2+), has been found to be a good substrate of bacterial Bacillus subtilis ferrochelatase. It is not known how ferrochelatase discriminates between different metal ion substrates. Structural analysis of B. subtilis ferrochelatase has shown that Tyr13 is an indirect ligand of Fe(2+) and a direct ligand of a copper mesoporphyrin product. A structure-based comparison revealed that Tyr13 aligns with a Met residue in the S. cerevisiae and human ferrochelatases. Tyr13 was changed to Met in the B. subtilis enzyme by site-directed mutagenesis. Enzymatic measurements showed that the modified enzyme inserted Co(2+) at a higher rate than the wild-type B. subtilis ferrochelatase, but it had lost the ability to use Cu(2+) as a substrate. Thus, the B. subtilis Tyr13Met ferrochelatase showed the same metal specificity as that of the ferrochelatases from S. cerevisiae and human.
|
|
| 5. |
- Karlberg, Tobias, et al.
(författare)
-
Porphyrin Binding and Distortion and Substrate Specificity in the Ferrochelatase Reaction: The Role of Active Site Residues.
- 2008
-
Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 1089-8638 .- 0022-2836. ; 378:5, s. 1074-1083
-
Tidskriftsartikel (refereegranskat)abstract
- The specific insertion of a divalent metal ion into tetrapyrrole macrocycles is catalyzed by a group of enzymes called chelatases. Distortion of the tetrapyrrole has been proposed to be an important component of the mechanism of metallation. We present the structures of two different inhibitor complexes: (1) N-methylmesoporphyrin (N-MeMP) with the His183Ala variant of Bacillus subtilis ferrochelatase; (2) the wild-type form of the same enzyme with deuteroporphyrin IX 2,4-disulfonic acid dihydrochloride (dSDP). Analysis of the structures showed that only one N-MeMP isomer out of the eight possible was bound to the protein and it was different from the isomer that was earlier found to bind to the wild-type enzyme. A comparison of the distortion of this porphyrin with other porphyrin complexes of ferrochelatase and a catalytic antibody with ferrochelatase activity using normal-coordinate structural decomposition reveals that certain types of distortion are predominant in all these complexes. On the other hand, dSDP, which binds closer to the protein surface compared to N-MeMP, does not undergo any distortion upon binding to the protein, underscoring that the position of the porphyrin within the active site pocket is crucial for generating the distortion required for metal insertion. In addition, in contrast to the wild-type enzyme, Cu(2+)-soaking of the His183Ala variant complex did not show any traces of porphyrin metallation. Collectively, these results provide new insights into the role of the active site residues of ferrochelatase in controlling stereospecificity, distortion and metallation.
|
|
| 6. |
- Shipovskov, Stepan, et al.
(författare)
-
Metallation of the transition-state inhibitor N-methyl mesoporphyrin by ferrochelatase: Implications for the catalytic reaction mechanism
- 2005
-
Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 1089-8638 .- 0022-2836. ; 352:5, s. 1081-1090
-
Tidskriftsartikel (refereegranskat)abstract
- Insertion of metals into various tetrapyrroles is catalysed by a group of enzymes called chelatases, e.g. nickel, cobalt, magnesium and ferrochelatase. It has been proposed that catalytic metallation includes distorting the porphyrin substrate by the enzyme towards a transition state-like geometry in which at least one of the pyrrole rings will be available for metal chelation. Here, we present a study of metal insertion into the transition-state inhibitor of protoporphyrin IX ferrochelatase, N-methyl mesoporphyrin (N-MeMP), by time-resolved crystallography and mass spectrometry with and without the presence of ferrochelatase. The results show that metallation of N-MeMP has a very limited effect on the conformation of the residues that participate in porphyrin and metal binding. These findings support theoretical data, which indicate that product release is controlled largely by the strain created by metal insertion into the distorted porphyrin. The results suggest that, similar to noncatalytic metallation of N-MeMP, the ferrochelatase-assisted metallation depends on the ligand exchange rate for the respective metal. Moreover, ferrochelatase catalyses insertion of Cu(II) and Zn(II) into N-MeMP with a rate that is about 20 times faster than non-enzymatic metallation in solution, suggesting that the catalytic strategy of ferrochelatase includes a stage of acceleration of the rate of ligand exchange for the metal substrate. The greater efficiency of N-MeMP metallation by Cu(II), as compared to Zn(II), contrasts with the Km values for Zn(II), (17 mu M) and Cu(II) (170 mu M) obtained for metallation of protoporphyrin IX. We suggest that this difference in metal specificity depends on the type of distortion imposed by the enzyme on protoporphyrin IX, which is different from the intrinsic non-planar distortion of N-MeMP. A mechanism of control of metal specificity by porphyrin distortion may be general for different chelatases, and may have common features with the mechanism of metal specificity in crown ethers.(c) 2005 Elsevier Ltd. All rights reserved.
|
|
| 7. |
- Skillbäck, Tobias, et al.
(författare)
-
A novel quantification-driven proteomic strategy identifies an endogenous peptide of pleiotrophin as a new biomarker of Alzheimer's disease
- 2017
-
Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7
-
Tidskriftsartikel (refereegranskat)abstract
- We present a new, quantification-driven proteomic approach to identifying biomarkers. In contrast to the identification-driven approach, limited in scope to peptides that are identified by database searching in the first step, all MS data are considered to select biomarker candidates. The endopeptidome of cerebrospinal fluid from 40 Alzheimer's disease (AD) patients, 40 subjects with mild cognitive impairment, and 40 controls with subjective cognitive decline was analyzed using multiplex isobaric labeling. Spectral clustering was used to match MS/MS spectra. The top biomarker candidate cluster (215% higher in AD compared to controls, area under ROC curve = 0.96) was identified as a fragment of pleiotrophin located near the protein's C-terminus. Analysis of another cohort (n = 60 over four clinical groups) verified that the biomarker was increased in AD patients while no change in controls, Parkinson's disease or progressive supranuclear palsy was observed. The identification of the novel biomarker pleiotrophin 151-166 demonstrates that our quantification-driven proteomic approach is a promising method for biomarker discovery, which may be universally applicable in clinical proteomics.
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
|
|
| 11. |
- Ahlberg, Erik, et al.
(författare)
-
"Vi klimatforskare stödjer Greta och skolungdomarna"
- 2019
-
Ingår i: Dagens nyheter (DN debatt). - 1101-2447.
-
Tidskriftsartikel (populärvet., debatt m.m.)abstract
- DN DEBATT 15/3. Sedan industrialiseringens början har vi använt omkring fyra femtedelar av den mängd fossilt kol som får förbrännas för att vi ska klara Parisavtalet. Vi har bara en femtedel kvar och det är bråttom att kraftigt reducera utsläppen. Det har Greta Thunberg och de strejkande ungdomarna förstått. Därför stödjer vi deras krav, skriver 270 klimatforskare.
|
|
| 12. |
- Anderson, Dan, 1943, et al.
(författare)
-
Parabolic similarity solutions of the Nonlinear Schrödinger and Wigner-Moyal equations
- 2008
-
Ingår i: in Proceedings of SIAM Conference on Nonlinear Waves and Coherent Structures, July 21-24, 2008, Rome, Italy, Society for Industrial and Applied Mathematics (SIAM), Philadelphia, PA, USA. ; , s. 56-
-
Konferensbidrag (refereegranskat)
|
|
| 13. |
- Andersson, Joel B. H., et al.
(författare)
-
Structural controls on sulphide (re)-distribution in Kiruna
- 2019
-
Ingår i: Proceedings of the 15th SGA Biennial Meeting, 27-30 August. - : University of Glasgow Publicity Services. ; , s. 115-118
-
Konferensbidrag (refereegranskat)abstract
- In this study, we investigate a potential structural control on sulphide distribution in phyllite from the Kiruna area, northern Norrbotten, Sweden. We use X-ray tomography and X-ray fluorescence analyzed in tandem on a 40 cm section of oriented drill core. Scanning Electron Microscopy and Energy Dispersive Spectrometry are used in combination with optical microscopy to analyze mineralogy and microstructures. The results show that sulphides are distributed along So bedding planes, re-distributed along S-2 axial planar cleavage planes, and trapped by F-2-hinge zones and shear bands visible as elevated sulphide concentrations. The results of this study underlines the strength of X-ray tomography to image 3D geological structures and their relation to mineral distributions.
|
|
| 14. |
- Arabi, C. Mas, et al.
(författare)
-
Localized structures formed through domain wall locking in cavity-enhanced second-harmonic generation
- 2020
-
Ingår i: Optics Letters. - : OPTICAL SOC AMER. - 0146-9592 .- 1539-4794. ; 45:20, s. 5856-5859
-
Tidskriftsartikel (refereegranskat)abstract
- We analyze the formation of localized structures in cavity-enhanced second-harmonic generation. We focus on the phase-matched limit, and consider that fundamental and generated waves have opposite signs of group velocity dispersion. We show that these states form due to the locking of domain walls connecting two stable homogeneous states of the system, and undergo collapsed snaking. We study the impact of temporal walk-off on the stability and dynamics of these localized states. (C) 2020 Optical Society of America
|
|
| 15. |
- Berglund, Per, et al.
(författare)
-
Linking Education and Research : A Roadmap for Higher Education Institutions at the Dawn of the Knowledge Society
- 2019
-
Ingår i: Linking education and research. - Basel, Switzerland : MDPI. ; , s. 11-33
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- In an era characterized by a move towards a “knowledge society”, universities are central in fostering “knowledgeability”, that is the reflexive understanding of knowledge in knowledge societies. The objective of “knowledgeability” can be met through creating a stronger link between education and research. Furthermore, overall student performance, for example in critical thinking and problem solving, can be improved if research-related activities are incorporated into the curriculum.The aim of this paper is to use international examples to discuss the research- education nexus from four different perspectives, namely context, policy, implementation and quality, with case studies from higher education institutions in Singapore and Sweden.We suggest that different integrative technologies can be used to enhance the links, but it will be essential to consider the inputs of training, service and support in using new technology. Interestingly, the act of evaluating the link between education and research will increase awareness of this linkage by stakeholders involved in both education and research. In turn the link can be strengthened, contributing to increased quality in both education and research.
|
|
| 16. |
- Boggio, J. M. Chavez, et al.
(författare)
-
Efficient Kerr soliton comb generation in micro-resonator with interferometric back-coupling
- 2022
-
Ingår i: Nature Communications. - : Nature Portfolio. - 2041-1723. ; 13:1
-
Tidskriftsartikel (refereegranskat)abstract
- Nonlinear Kerr micro-resonators have enabled fundamental breakthroughs in the understanding of dissipative solitons, as well as in their application to optical frequency comb generation. However, the conversion efficiency of the pump power into a soliton frequency comb typically remains below a few percent. We fabricate and characterize a hybrid Mach-Zehnder ring resonator geometry, consisting of a micro-ring resonator embedded in an additional cavity with twice the optical path length of the ring. The resulting interferometric back coupling enables to achieve an unprecedented control of the pump depletion: pump-to-frequency comb conversion efficiencies of up to 55% of the input pump power is experimentally demonstrated with a soliton crystal comb. We assess the robustness of the proposed on-chip geometry by generating a large variety of dissipative Kerr soliton combs, which require a lower amount of pump power to be accessed, when compared with an isolated micro-ring resonator with identical parameters. Micro-resonators with feedback enable accessing new regimes of coherent soliton comb generation, and are well suited for comb applications in astronomy, spectroscopy and telecommunications. Increasing the conversion efficiency of soliton crystals will enable further application of optical frequency comb. Here the authors engineer an hybrid Mach-Zehnder micro-ring resonator to achieve 80% pump-to-comb conversion efficiency based on dissipative Kerr solitons.
|
|
| 17. |
- Boggio, J. M. Chavez, et al.
(författare)
-
Frequency comb generation in silicon nitride resonators with amplitude modulated pump
- 2021
-
Ingår i: LASER RESONATORS, MICRORESONATORS, AND BEAM CONTROL XXIII. - : SPIE-INT SOC OPTICAL ENGINEERING. - 9781510641808
-
Konferensbidrag (refereegranskat)abstract
- We investigate frequency comb generation in silicon nitride ring resonators by using a pump subject to a weak amplitude modulation. We show that a partial locking is obtained when the external modulation frequency differs from the resonator free-spectral-range by up to hundreds of MHz.
|
|
| 18. |
- Bramer, Tobias, et al.
(författare)
-
Implications of regular solution theory on the release mechanism of catanionic mixtures from gels
- 2009
-
Ingår i: Colloids and Surfaces B. - : Elsevier BV. - 0927-7765 .- 1873-4367. ; 71:2, s. 214-225
-
Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to apply the regular solution theory of mixed micelles to gain new insights on the drug release mechanism, when using catanionic mixtures as a method of obtaining prolonged release from gels. Synergistic effects were investigated at equilibrium and quantified in terms of regular solution theory interaction parameters. The drug release from catanionic aggregates was studied both in a polymer free environment, using dialysis membranes, and in gels, using a modified LISP paddle method. The drug release kinetics was modelled theoretically by combining the regular solution theory with Fick's diffusion laws assuming a contribution to the transport only from monomeric species (stationary aggregates). The theoretical predictions were found to be in reasonably good agreement with experiments. An analysis of the calculated distribution of species between aggregated and monomeric states was shown to provide further insights into the release mechanism.
|
|
| 19. |
- Bramer, Tobias, 1974-
(författare)
-
Prolonged Drug Release from Gels, using Catanionic Mixtures
- 2007
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The use of catanionic drug-surfactant mixtures was proven to be an efficient novel method of obtaining prolonged drug release from gels. It was shown that various commonly used drug compounds are able to form catanionic mixtures together with oppositely charged surfactants. These mixtures exhibited interesting phase behaviour, where, among other structures, vesicles and large worm-like or branched micelles were found. The size of these aggregates makes them a potential means of prolonging the drug release from gels, as only monomer drugs in equilibrium with larger aggregates were readily able to diffuse through the gel. When the diffusion coefficient for drug release from the formulation based upon a catanionic mixture was compared to that obtained for the drug substance and gel alone, the coefficient was some 10 to 100 times smaller.The effects of changes in the pH and ionic strength on the catanionic aggregates was also investigated, and this method of prolonging the release was found to be quite resilient to variations in both. Although the phase behaviour was somewhat affected, large micelles and vesicles were still readily found. The drug release was significantly prolonged even under physiological conditions, that is, at a pH of 7.4 and an osmolality corresponding to 0.9% NaCl.Surfactants of low irritancy, capric and lauric acid, may successfully be used instead of the more traditional surfactants, such as sodium lauryl sulfate (SDS), and prolonged release can still be obtained with ease.Some attempts to deduce the release mechanism from the proposed systems have also been made using transient current measurements, dielectric spectroscopy, and modelling of the release using the regular solution theory. In these studies, the previous assumptions made concerning the mechanism responsible for the release were confirmed to a large extent. Only small amounts of the drug existed in monomer form, and most seemed to form large catanionic aggregates with the oppositely charged surfactant.
|
|
| 20. |
- Bridel, Claire, et al.
(författare)
-
Diagnostic Value of Cerebrospinal Fluid Neurofilament Light Protein in Neurology : A Systematic Review and Meta-analysis
- 2019
-
Ingår i: JAMA Neurology. - : American Medical Association (AMA). - 2168-6149 .- 2168-6157. ; 76:9, s. 1035-1048
-
Forskningsöversikt (refereegranskat)abstract
- Importance Neurofilament light protein (NfL) is elevated in cerebrospinal fluid (CSF) of a number of neurological conditions compared with healthy controls (HC) and is a candidate biomarker for neuroaxonal damage. The influence of age and sex is largely unknown, and levels across neurological disorders have not been compared systematically to date.Objectives To assess the associations of age, sex, and diagnosis with NfL in CSF (cNfL) and to evaluate its potential in discriminating clinically similar conditions.Data Sources PubMed was searched for studies published between January 1, 2006, and January 1, 2016, reporting cNfL levels (using the search terms neurofilament light and cerebrospinal fluid) in neurological or psychiatric conditions and/or in HC.Study Selection Studies reporting NfL levels measured in lumbar CSF using a commercially available immunoassay, as well as age and sex.Data Extraction and Synthesis Individual-level data were requested from study authors. Generalized linear mixed-effects models were used to estimate the fixed effects of age, sex, and diagnosis on log-transformed NfL levels, with cohort of origin modeled as a random intercept.Main Outcome and Measure The cNfL levels adjusted for age and sex across diagnoses.Results Data were collected for 10 059 individuals (mean [SD] age, 59.7 [18.8] years; 54.1% female). Thirty-five diagnoses were identified, including inflammatory diseases of the central nervous system (n = 2795), dementias and predementia stages (n = 4284), parkinsonian disorders (n = 984), and HC (n = 1332). The cNfL was elevated compared with HC in a majority of neurological conditions studied. Highest levels were observed in cognitively impaired HIV-positive individuals (iHIV), amyotrophic lateral sclerosis, frontotemporal dementia (FTD), and Huntington disease. In 33.3% of diagnoses, including HC, multiple sclerosis, Alzheimer disease (AD), and Parkinson disease (PD), cNfL was higher in men than women. The cNfL increased with age in HC and a majority of neurological conditions, although the association was strongest in HC. The cNfL overlapped in most clinically similar diagnoses except for FTD and iHIV, which segregated from other dementias, and PD, which segregated from atypical parkinsonian syndromes.Conclusions and Relevance These data support the use of cNfL as a biomarker of neuroaxonal damage and indicate that age-specific and sex-specific (and in some cases disease-specific) reference values may be needed. The cNfL has potential to assist the differentiation of FTD from AD and PD from atypical parkinsonian syndromes.
|
|
| 21. |
- Cattani, F., et al.
(författare)
-
Multihump soliton-like structures in interactions of lasers and Bose-Einstein condensates
- 2011
-
Ingår i: Europhysics Letters. - : IOP Publishing. - 0295-5075 .- 1286-4854. ; 94:5, s. 53003-
-
Tidskriftsartikel (refereegranskat)abstract
- An investigation is made of multihump and periodic solutions of the semiclassical coupled equations describing laser radiation copropagating with a Bose-Einstein condensate. Solutions reminiscent of optical vector solitons have been found and have been used to gain understanding of the dynamics observed in the numerical simulations, in particular to shed light on the phenomenon of jet emission from a condensate interacting with a laser.
|
|
| 22. |
- Ceoldo, D., et al.
(författare)
-
Kerr frequency combs in a bichromatically pumped nonlinear fiber ring cavity
- 2016
-
Ingår i: 42nd European Conference on Optical Communication, ECOC 2016; Dusseldorf; Germany; 18 September 2016 through 22 September 2016. - 9783800742745 ; , s. 538-540
-
Konferensbidrag (refereegranskat)abstract
- We report numerical and experimental studies of four-wave mixing processes emerging from dual-frequency pumping of a passive fibre ring cavity. We observe the emission of a periodic train of nearly-background-free soliton pulses associated with Kerr frequency combs.
|
|
| 23. |
|
|
| 24. |
- Cuapio, Angelica, et al.
(författare)
-
NK cell frequencies, function and correlates to vaccine outcome in BNT162b2 mRNA anti-SARS-CoV-2 vaccinated healthy and immunocompromised individuals
- 2022
-
Ingår i: Molecular Medicine. - : BioMed Central (BMC). - 1076-1551 .- 1528-3658. ; 28:1
-
Tidskriftsartikel (refereegranskat)abstract
- Adaptive immune responses have been studied extensively in the course of mRNA vaccination against COVID-19. Considerably fewer studies have assessed the effects on innate immune cells. Here, we characterized NK cells in healthy individuals and immunocompromised patients in the course of an anti-SARS-CoV-2 BNT162b2 mRNA prospective, open-label clinical vaccine trial. See trial registration description in notes. Results revealed preserved NK cell numbers, frequencies, subsets, phenotypes, and function as assessed through consecutive peripheral blood samplings at 0, 10, 21, and 35 days following vaccination. A positive correlation was observed between the frequency of NKG2C+ NK cells at baseline (Day 0) and anti-SARS-CoV-2 Ab titers following BNT162b2 mRNA vaccination at Day 35. The present results provide basic insights in regards to NK cells in the context of mRNA vaccination, and have relevance for future mRNA-based vaccinations against COVID-19, other viral infections, and cancer.Trial registration: The current study is based on clinical material from the COVAXID open-label, non-randomized prospective clinical trial registered at EudraCT and clinicaltrials.gov (no. 2021–000175-37). Description: https://clinicaltrials.gov/ct2/show/NCT04780659?term=2021-000175-37&draw=2&rank=1.
|
|
| 25. |
- Doving, Kjell B., et al.
(författare)
-
Visualizing a set of olfactory sensory neurons responding to a bile salt
- 2011
-
Ingår i: Journal of Experimental Biology. - : The Company of Biologists. - 0022-0949 .- 1477-9145. ; 214:1, s. 80-87
-
Tidskriftsartikel (refereegranskat)abstract
- In the present study, we exposed the olfactory epithelia of crucian carp, Carassius carassius, and brown trout, Salmo trutta, to dextran coupled with Alexa dyes together with odorants. Dye uptake was severely reduced after pre-exposure to nocodazole, an inhibitor of microtubule polymerization that impairs endocytosis, supporting the hypothesis that odour-activated olfactory receptor molecules undergo endocytosis. Application of the bile acid taurolithocholate, a potent and specific odorant for fish, resulted in the labelling of a sparse (less than 3%) cell population with the typical morphology of ciliated sensory neurons (CSNs) - long dendrites and cell somata deep in the sensory epithelium. The dye was distributed throughout the sensory neuron, also revealing axons and target glomeruli. Stained axons redistribute at the entrance of the olfactory bulb and terminate in two small target areas, a dorsal and a medial one. These results are consistent with the notion that taurolithocholate is detected specifically by a few ciliated sensory neurons. Application of the olfactory epithelium of brown trout to bile acid stained cells with the appearance of CSNs. Application of an alarm agonist, hypxanthine-3-N-oxide, to crucian carp olfactory organ caused staining of another set of sensory neurons. Furthermore, our results show that odour-induced uptake of a dye can serve to identify the subtype of olfactory sensory neurons responding to a particular odorant, and to pinpoint the target regions of these neurons in the olfactory bulb as a first step to elucidating the neuronal network responding to a particular odour.
|
|
| 26. |
|
|
| 27. |
- Ekström, Cecilia, 1989-
(författare)
-
Enabling and Coercive Control : Coexistence in the Case of Banking
- 2018
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis focuses on subjects of control and attitudinal outcomes of formalized control in organizations. Previous research have concluded contradicting results of whether formalized control is positive or negative for the employees and propose that not only degree of formalization, but also type of formalization, can explain attitudinal outcomes.With the theoretical perspective of Adler and Borys’ concepts of enabling and coercive types of control, this thesis explore the concepts and practices of enabling and coercive control, and their relationships with attitudinal outcomes. This is done with a focus on the banking industry, which serve as a case of an extensively regulated context. An assumption put forth in this thesis is that the context in which individuals are part must be considered in the study of attitudinal outcomes of control.The analysis show that both enabling and coercive control can be found in banking, for example in business plans and regulations. However, the picture emerging is more complex than enabling control leading to positive attitudes, and coercive control leading to negative attitudes. Also, coexistence of enabling and coercive control is responded to with decoupling and acquiescence, and by drawing on global transparency.Based on these findings, together with theoretical elaborations, this thesis contribute to the literature of enabling and coercive control in a number of ways. First, it make explicit central concepts and relationships within the theory, such as design vs. perception, the role of zone of indifference as an outcome of control, and enabling and coercive control as dual roles or qual-ities of control. Moreover, this thesis suggests that multiple-level explanations to attitudinal outcomes of control, where contextual and institutional structures are considered, helps us understand attitudes to control in this context. Lastly, this thesis contribute to the notion of coexistence of enabling and coercive control by showing that coexistence can be simultaneous systems, and simultaneous cognitions, where a control can be perceived as both enabling and coercive at the same time.
|
|
| 28. |
- Ekvall, Mikael T., et al.
(författare)
-
Behavioural responses to co-occurring threats of predation and ultraviolet radiation in Daphnia
- 2020
-
Ingår i: Freshwater Biology. - : Wiley. - 0046-5070 .- 1365-2427. ; 65:9, s. 1509-1517
-
Tidskriftsartikel (refereegranskat)abstract
- Organisms in the wild are faced with multiple threats and a common response is a change in behaviour. To disentangle responses to several threats, we exposed two differently sized species of the freshwater invertebrate Daphnia to solar ultraviolet radiation (UVR) and predation from either moving pelagic or benthic ambush predators. Using an advanced nanotechnology-based method, we tracked the three-dimensional movements of those mm-sized animals at the individual level. Each behavioural trial was performed both under conditions resembling night (no UVR) and day (UVR) and we examined patterns of the depth distribution and swimming speed by Daphnia across three treatments: no predator (control); bottom-dwelling damselfly (Calopteryx sp.); and fish (stickleback, Pungitius pungitius) predators. We also quantified the actual predation rate by the two predators on the two Daphnia species, Daphnia manga and Daphnia pulex. We show that individual Daphnia are able to identify predators with different feeding habitats, rank multiple and simultaneously occurring risks and respond in accordance with the actual threat; complex responses that are generally associated with larger animals. In a broader context, our results highlight and quantify how a cocktail of everyday threats is perceived and handled by invertebrates, which advances our understanding of species distribution in space and time, and thereby of population dynamics and ecosystem function in natural ecosystems.
|
|
| 29. |
- Elgán, Tobias H., et al.
(författare)
-
A web-based group course intervention for 15-25-year-olds whose parents have substance use problems or mental illness : Study protocol for a randomized controlled trial
- 2016
-
Ingår i: BMC Public Health. - : Springer Science and Business Media LLC. - 1471-2458. ; 16:1
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Depending on the definitions used, between 5 and 20 % of all Swedish children grow up with at least one parent suffering from alcohol problems, while 6 % have at least one parent who has received inpatient psychiatric care, conditions that may affect the children negatively. Nine out of ten Swedish municipalities therefore provide support resources, but less than 2 % of these children are reached by such support. Delivering intervention programs via the Internet is a promising strategy. However, web-based programs targeting this at-risk group of children are scarce. We have previously developed a 1.5-h-long web-based self-help program, Alcohol & Coping, which appears to be effective with regards to adolescents' own alcohol consumption. However, there is a need for a more intense program, and therefore we adapted Kopstoring, a comprehensive Dutch web-based psycho-educative prevention program, to fit the Swedish context. The purpose of the program, which in Swedish has been called Grubbel, is to strengthen protective factors, such as coping skills and psychological well-being, prevent the development of psychological disorders, and reduce alcohol consumption. Methods/design: The aim of the current study is to evaluate the effectiveness of Grubbel, which targets 15-25-year-olds whose parents have substance use problems and/or mental illness. Specific research questions relate to the participants' own coping strategies, mental health status and substance use. The study was initiated in the spring of 2016 and uses a two-armed RCT design. Participants will be recruited via social media and also through existing agencies that provide support to this target group. The assessment will consist of a baseline measurement (t0) and three follow-ups after six (t1), 12 (t2), and 24 months (t3). Measures include YSR, CES-DC, Ladder of Life, Brief COPE, AUDIT-C, and WHOQOL-BREF. Discussion: Studies have revealed that the majority of children whose parents have substance use or mental health problems are not reached by the existing support. Thus, there is an urgent need to develop, implement, and evaluate novel intervention programs and disseminate successful programs to a broader audience. This study, investigating the effects of a web-based intervention, therefore makes an important contribution to this field of research. Trial registration: ISRCTN10099247. Retrospectively registered on August 31, 2016.
|
|
| 30. |
- Elgan, Tobias H., et al.
(författare)
-
Design of a Web-based individual coping and alcohol-intervention program (web-ICAIP) for children of parents with alcohol problems: study protocol for a randomized controlled trial
- 2012
-
Ingår i: BMC Public Health. - : Springer Science and Business Media LLC. - 1471-2458. ; 12
-
Tidskriftsartikel (refereegranskat)abstract
- Background: It has been estimated that approximately 20% of all Swedish children grow up with parents having alcohol problems, which may result in negative outcomes among these children. Therefore, most Swedish municipalities provide resources for support, but at the same time figures reveal that not even 2% receive support, mainly due to difficulties in identifying and recruiting these children into support programs. Delivering intervention programs to children and adolescents via the Internet seems a promising strategy, but to date, the number of web-based interventions aimed at this target group is very scarce. We have therefore developed a novel internet-delivered therapist assisted self-management intervention called the web-ICAIP (Individual Coping and Alcohol Intervention Program) for adolescents having parents with alcohol problems. The purpose of the program is to strengthen adolescents' coping behavior, improve their mental health, and postponing the onset or decreasing risky alcohol consumption. This paper describes the web-ICAIP and the design of a randomized controlled trial (RCT) to measure the efficacy of this intervention. Methods/Design: The RCT will include at least 183 adolescents (15-19 year old) who will be randomly allocated to two conditions where one group has access to the web-ICAIP and the other is a waiting list control group. Participants will be recruited from websites containing information and facts for adolescents about alcohol and other drugs. Possible participants will be screened using the short version of the Children of Alcoholics Screening Test (CAST-6). The assessment consists of a baseline and two follow-up measurements taking place after two and six months, respectively. The primary outcomes include the Center for Epidemiological Studies Depression Scale (CES-DC), a coping behavior scale, and also the short version of the Alcohol Use Disorders Identification Test (AUDIT-C). Additional outcomes include the "Ladder of life" which measures overall life satisfaction and questions concerning program adherence. Discussion: There is an urgent need for developing and evaluating web-based intervention programs which target children having parents with alcohol problems. This study will therefore make an important contribution to this novel field of research.
|
|
| 31. |
- Erhardt, Tobias, et al.
(författare)
-
High-resolution aerosol concentration data from the Greenland NorthGRIP and NEEM deep ice cores
- 2022
-
Ingår i: Earth System Science Data. - : Copernicus GmbH. - 1866-3508 .- 1866-3516. ; 14:3, s. 1215-1231
-
Tidskriftsartikel (refereegranskat)abstract
- Records of chemical impurities from ice cores enable us to reconstruct the past deposition of aerosols onto polar ice sheets and alpine glaciers. Through this they allow us to gain insight into changes of the source, transport and deposition processes that ultimately determine the deposition flux at the coring location. However, the low concentrations of the aerosol species in the ice and the resulting high risk of contamination pose a formidable analytical challenge, especially if long, continuous and highly resolved records are needed. Continuous flow analysis, CFA, the continuous melting, decontamination and analysis of ice-core samples has mostly overcome this issue and has quickly become the de facto standard to obtain high-resolution aerosol records from ice cores after its inception at the University of Bern in the mid-1990s.Here, we present continuous records of calcium (Ca2+), sodium (Na+), ammonium (NH+4), nitrate (NO-3) and electrolytic conductivity at 1 mm depth resolution from the NGRIP (North Greenland Ice Core Project) and NEEM (North Greenland Eemian Ice Drilling) ice cores produced by the Bern Continuous Flow Analysis group in the years 2000 to 2011 (Erhardt et al., 2021). Both of the records were previously used in a number of studies but were never published in full 1 mm resolution. Alongside the 1 mm datasets we provide decadal averages, a detailed description of the methods, relevant references, an assessment of the quality of the data and its usable resolution. Along the way we will also give some historical context on the development of the Bern CFA system.
|
|
| 32. |
- Erkintalo, M., et al.
(författare)
-
Frequency combs in quadratically nonlinear resonators
- 2016
-
Ingår i: Optics InfoBase Conference Papers. - 2162-2701. - 9781943580170 ; , s. paper NM3A.1-
-
Konferensbidrag (refereegranskat)abstract
- We describe the physics and modelling of frequency combs and corresponding temporal patterns in coherently driven, quadratically nonlinear resonators.
|
|
| 33. |
- Erkintalo, M., et al.
(författare)
-
Numerical modelling of frequency comb generation in nonlinear resonators
- 2016
-
Ingår i: 16th International Conference on Numerical Simulation of Optoelectronic Devices, NUSOD 2016, Sydney, Australia, 11-15 July 2016. - 2158-3234. - 9781467386036 ; , s. 187-188
-
Konferensbidrag (refereegranskat)abstract
- We present a comprehensive review of recent work on numerical modelling of frequency comb generation in coherently-driven nonlinear resonators. Specifically, we discuss modelling and nonlinear dynamics of frequency combs generated in Kerr nonlinear microresonators, emphasizing links to similar dynamics studied in macroscopic fibre cavities. In addition to established Kerr frequency comb generators, we will also discuss emerging comb generation platforms based on continuously-driven quadratically nonlinear resonators.
|
|
| 34. |
- Gao, Yu, et al.
(författare)
-
Immunodeficiency syndromes differentially impact the functional profile of SARS-CoV-2-specific T cells elicited by mRNA vaccination
- 2022
-
Ingår i: Immunity. - : Elsevier. - 1074-7613 .- 1097-4180. ; 55:9, s. 1732-1746.e5
-
Tidskriftsartikel (refereegranskat)abstract
- Many immunocompromised patients mount suboptimal humoral immunity after SARS-CoV-2 mRNA vaccination. Here, we assessed the single-cell profile of SARS-CoV-2-specific T cells post-mRNA vaccination in healthy individuals and patients with various forms of immunodeficiencies. Impaired vaccine-induced cell-mediated immunity was observed in many immunocompromised patients, particularly in solid-organ transplant and chronic lymphocytic leukemia patients. Notably, individuals with an inherited lack of mature B cells, i.e., X-linked agammaglobulinemia (XLA) displayed highly functional spike-specific T cell responses. Single-cell RNA-sequencing further revealed that mRNA vaccination induced a broad functional spectrum of spike-specific CD4+ and CD8+ T cells in healthy individuals and patients with XLA. These responses were founded on polyclonal repertoires of CD4+ T cells and robust expansions of oligoclonal effector-memory CD45RA+ CD8+ T cells with stem-like characteristics. Collectively, our data provide the functional continuum of SARS-CoV-2-specific T cell responses post-mRNA vaccination, highlighting that cell-mediated immunity is of variable functional quality across immunodeficiency syndromes.
|
|
| 35. |
- Gasch, Tobias, et al.
(författare)
-
Concrete Support Structure for Hydroelectric Generators Subjected to Rotor Dynamic Loads
- 2014
-
Ingår i: International Symposium on Dams in a Global Enviromental Challange. - Bali.
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- In earlier times, the generators of the hydropower plants ran more or less continuously, while nowadays there are many planned starts and stops. The hydropower stations are thereby, due to the new pattern of operation, subjected to loads that they were not originally designed for. The aim of this study is to understand the complex interaction between the power generating system and the supporting concrete structure, during this new operational pattern.During inspections, cracks were discovered in the concrete structure of the power house, near the stator and rotor spider supports, at several hydropower stations in Sweden. In a previous phase of this project it was shown that these cracks initiated due to the combined effect of drying shrinkage, mechanical loads and variations in temperature due to starts and stops. Cracking of the concrete structure reduces its stiffness, which may result in larger loads acting on the structure and vibrations exceeding the unit’s strict tolerance limits.In this part of the study, the behaviour of a concrete support structure subjected to rotor dynamic loads during normal operation has been studied. A detailed 3D numerical model has been developed which include hydropower unit. The results of this study show that a reduced structural stiffness of the concrete support structure, due to cracking, influences the behaviour of the rotating system.
|
|
| 36. |
- Gasch, Tobias, et al.
(författare)
-
Cracking in the concrete foundation for hydropower generators : Part II
- 2013
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- An extensive program for improvement of the hydropower plants in Sweden is currently on-going. The aims are to secure future production and to maintain and further develop an already high dam safety.During inspection, cracks were discovered in the concrete foundation, near the stator and rotor spider supports, at some hydropower stations in Sweden. The cracks were believed to be related to new patterns for generator operation, thereby changing the dynamic loading of the stator and rotor spider supports. Previously the generators ran continuously, while nowadays there are an increased number of stops and starts, sometimes even several times during one day. Increased dynamic forces due to runaways, and also other dynamic events such as emergency stops, may also contribute to increased stress levels and cracking of the foundation. Furthermore, although extreme loads such as short circuits of the generator seldom occurs, the influence on the dynamic forces acting on the supporting structure and concrete foundation may be strongly influenced during such events.The objective of this study is to understand the complex interaction between the power generating system (stator, rotor, turbine, etc.) and its supporting concrete structure. It is important from a dam safety perspective to determine the causes of existing structural cracks in the foundation. Furthermore, to be able to predict further crack propagation of the concrete foundation will help to determine future maintenance requirements.A three dimensional non-linear finite element model developed earlier was used to evaluate a methodology for analyses of the interaction between the generator and the concrete foundation. The influence of cracks in the concrete foundation was investigated by including the fracture pattern obtained in earlier FE analyses of time-dependent thermal and moisture gradients. These analyses showed that the drying shrinkage induced cracking inside the concrete foundation and especially close to the supports of the stator and the rotor spider. The obtained fracture pattern for the previous analysis was used as input for this study, with the concrete foundation’s changed structural properties and their influence on the interaction with the generator considered in the analyses. Furthermore, deadweight and operational load were also included in the analyses.The study show that FE models with a cracked concrete foundation can be used to analyse structural interaction betwee foundation and generator components during operation of a hydro power generator. The crack pattern can be determined by FE analyses, or by in-situ measurements of existing concrete cracks for a specific concrete foundation. The analyses show that further studies are needed regarding the combined effects from thermo-mechanical loads, drying shrinkage, creep and dynamical loads caused by the generator. The combined effects may further increase the stress levels for the concrete foundation, especially locally near perforations, and stator and rotor spider supports. These analyses should be performed with an increased numerical resolution for both the concrete foundation and the supporting structure for the generator, with an increased accuracy for the local stress variations near perforations of the foundation and also at the supports for the generator. This research area will be further investigated within a recently started research project at KTH, financed by the Swedish Hydropower Centre.
|
|
| 37. |
- Gautam, Rekha, et al.
(författare)
-
Nonlinear optical response and self-trapping of light in biological suspensions
- 2020
-
Ingår i: ADVANCES IN PHYSICS-X. - : TAYLOR & FRANCIS LTD. - 2374-6149. ; 5:1
-
Forskningsöversikt (refereegranskat)abstract
- In the past decade, the development of artificial materials exhibiting novel optical properties has become a major scientific endeavor. One particularly interesting system is synthetic soft matter, which plays a central role in numerous fields ranging from life sciences, chemistry to condensed matter and biophysics. In this paper, we review briefly the optical force-induced nonlinearities in colloidal suspensions, which can give rise to nonlinear self-trapping of light for enhanced propagation through otherwise highly scattering media such as dielectric and plasmonic nanosuspensions. We then focus on discussing our recent work with respect to nonlinear biological suspensions, including self-trapping of light in colloidal suspensions of marine bacteria and red blood cells, where the nonlinear response is largely attributed to the optical forces acting on the cells. Although it is commonly believed that biological media cannot exhibit high optical non-linearity, self-focusing of light and formation of soliton-like wave-guides in bio-soft matter have been observed. Furthermore, we present preliminary results on biological waveguiding and sensing, and discuss some perspectives towards biomedical applications. The concept may be developed for subsequent studies and techniques in situations when low scattering and deep penetration of light is desired.
|
|
| 38. |
- Gautam, Rekha, et al.
(författare)
-
Optical force-induced nonlinearity and self-guiding of light in human red blood cell suspensions
- 2019
-
Ingår i: Light. - : CHINESE ACAD SCIENCES, CHANGCHUN INST OPTICS FINE MECHANICS AND PHYSICS. - 2095-5545 .- 2047-7538. ; 8
-
Tidskriftsartikel (refereegranskat)abstract
- Osmotic conditions play an important role in the cell properties of human red blood cells (RBCs), which are crucial for the pathological analysis of some blood diseases such as malaria. Over the past decades, numerous efforts have mainly focused on the study of the RBC biomechanical properties that arise from the unique deformability of erythrocytes. Here, we demonstrate nonlinear optical effects from human RBCs suspended in different osmotic solutions. Specifically, we observe self-trapping and scattering-resistant nonlinear propagation of a laser beam through RBC suspensions under all three osmotic conditions, where the strength of the optical nonlinearity increases with osmotic pressure on the cells. This tunable nonlinearity is attributed to optical forces, particularly the forward-scattering and gradient forces. Interestingly, in aged blood samples (with lysed cells), a notably different nonlinear behavior is observed due to the presence of free hemoglobin. We use a theoretical model with an optical force-mediated nonlocal nonlinearity to explain the experimental observations. Our work on light self-guiding through scattering bio-soft-matter may introduce new photonic tools for noninvasive biomedical imaging and medical diagnosis.
|
|
| 39. |
- Glimelius, Bengt, et al.
(författare)
-
U-CAN : a prospective longitudinal collection of biomaterials and clinical information from adult cancer patients in Sweden.
- 2018
-
Ingår i: Acta Oncologica. - : Taylor & Francis. - 0284-186X .- 1651-226X. ; 57:2, s. 187-194
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Progress in cancer biomarker discovery is dependent on access to high-quality biological materials and high-resolution clinical data from the same cases. To overcome current limitations, a systematic prospective longitudinal sampling of multidisciplinary clinical data, blood and tissue from cancer patients was therefore initiated in 2010 by Uppsala and Umeå Universities and involving their corresponding University Hospitals, which are referral centers for one third of the Swedish population.Material and Methods: Patients with cancer of selected types who are treated at one of the participating hospitals are eligible for inclusion. The healthcare-integrated sampling scheme encompasses clinical data, questionnaires, blood, fresh frozen and formalin-fixed paraffin-embedded tissue specimens, diagnostic slides and radiology bioimaging data.Results: In this ongoing effort, 12,265 patients with brain tumors, breast cancers, colorectal cancers, gynecological cancers, hematological malignancies, lung cancers, neuroendocrine tumors or prostate cancers have been included until the end of 2016. From the 6914 patients included during the first five years, 98% were sampled for blood at diagnosis, 83% had paraffin-embedded and 58% had fresh frozen tissues collected. For Uppsala County, 55% of all cancer patients were included in the cohort.Conclusions: Close collaboration between participating hospitals and universities enabled prospective, longitudinal biobanking of blood and tissues and collection of multidisciplinary clinical data from cancer patients in the U-CAN cohort. Here, we summarize the first five years of operations, present U-CAN as a highly valuable cohort that will contribute to enhanced cancer research and describe the procedures to access samples and data.
|
|
| 40. |
- Gregersen, Henrik, et al.
(författare)
-
Carfilzomib and dexamethasone maintenance following salvage ASCT in multiple myeloma : A randomised phase 2 trial by the Nordic Myeloma Study Group
- 2022
-
Ingår i: European Journal of Haematology. - : John Wiley & Sons. - 0902-4441 .- 1600-0609. ; 108:1, s. 34-44
-
Tidskriftsartikel (refereegranskat)abstract
- Objective We investigated the efficacy and safety of carfilzomib-containing induction before salvage high-dose melphalan with autologous stem-cell transplantation (salvage ASCT) and maintenance with carfilzomib and dexamethasone after salvage ASCT in multiple myeloma. Methods This randomised, open-label, phase 2 trial included patients with first relapse of multiple myeloma after upfront ASCT who were re-induced with four cycles of carfilzomib, cyclophosphamide and dexamethasone. Two months after salvage, ASCT patients were randomised to either observation or maintenance therapy with iv carfilzomib 27 -> 56 mg/sqm and p.o. dexamethasone 20 mg every second week. The study enrolled 200 patients of which 168 were randomised to either maintenance with carfilzomib and dexamethasone (n = 82) or observation (n = 86). Results Median time to progression (TTP) after randomisation was 25.1 months (22.5-NR) in the carfilzomib-dexamethasone maintenance group and 16.7 months (14.4-21.8) in the control group (HR 0.46, 95% CI 0.30-0.71; P = .0004). The most common adverse events during maintenance were thrombocytopenia, anaemia, hypertension, dyspnoea and bacterial infections. Conclusion In summary, maintenance therapy with carfilzomib and dexamethasone after salvage ASCT prolonged TTP with 8 months. The maintenance treatment was in general well-tolerated with manageable toxicity.
|
|
| 41. |
- Hansson, Anders, 1953-, et al.
(författare)
-
Working together--primary care doctors' and nurses' attitudes to collaboration.
- 2010
-
Ingår i: Scandinavian journal of public health. - : SAGE Publications. - 1651-1905 .- 1403-4948. ; 38:1, s. 78-85
-
Tidskriftsartikel (refereegranskat)abstract
- Multidisciplinary teamwork is recommended for various disorders and it has been suggested that it is a way to meet the new challenges and demands facing general practitioners (GPs) in modern society. Attempts to introduce the method in primary care have failed partly due to GPs' unwillingness to participate. The aim of this study was to measure attitudes towards collaboration among GPs and district nurses (DN) and to investigate whether there is a correlation between a positive attitude toward collaboration and high self-esteem in the professional role.
|
|
| 42. |
- Hansson, Julia, et al.
(författare)
-
Costs for reducing GHG emissions from road and air transport with biofuels and electrofuels
- 2023
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Renewable fuels for transport are needed to reach future climate targets. However, the potential future role of different biofuels, hydrogen, and electrofuels (produced by electricity, water, and CO2) in different transportation sectors remains uncertain. Increased knowledge about the preconditions for different renewable fuels for road and air transport to contribute to the transformation of the transport sector is needed to ensure the transformation is done in a climate- and cost-effective way. The CO2 abatement cost, i.e., the cost of reducing a certain amount of greenhouse gas (GHG) emissions is central from both a societal and business perspective, the latter partly due to the design of the Swedish reduction obligation system.The abatement cost of a specific fuel value chain depends on the fuel production cost and the GHG reduction provided by the fuel. This report provides an updated summary of the CO2 abatement costs for various types of biofuels and electrofuels for road transport and aviation, relevant in a Swedish context. Fuel production costs and GHG performance (well to wheel) for the selected renewable fuel pathways are mapped based on published data. The estimated CO2 abatement cost ranges from -0.37 to 4.03 SEK/kg CO2-equivalent. Methane from anaerobic digestion of sewage sludge and ethanol from fermentation of sugarcane and maize end up with negative CO2 abatement cost given the assumptions made, meaning it is more economically beneficial to use than its fossil counterpart.Electrofuels pathways (particularly diesel and aviation fuels) have, on the other hand, relatively high CO2 abatement costs. Also, so-called bio-electrofuels produced from biogenic excess CO2 from biofuel production and electricity linked to biofuel production generally have higher CO2 abatement costs than the corresponding forest biomass-based biofuel pathway. For forest biomass-based biofuels, bio-electrofuels and electrofuels, methanol, and methane pathways in general have somewhat lower CO2 abatement costs than hydrocarbon-based fuels (gasoline, diesel, and aviation fuel).Since most of the assessed renewable fuel pathways achieve substantial GHG emission reduction compared to fossil fuels, the fuel production cost is, in general, more important than the GHG performance to achieve a low CO2 abatement cost. The production cost for fossil fuels also influences the CO2 abatement cost to a large extent. More estimates of cost and GHG performance for gasification of waste-based pathways are needed and for certain pathways under development (e.g., including hydropyrolysis).
|
|
| 43. |
- Hansson, Karl, 1985, et al.
(författare)
-
Expanding the cerebrospinal fluid endopeptidome
- 2017
-
Ingår i: Proteomics. - : Wiley. - 1615-9853. ; 17:5
-
Tidskriftsartikel (refereegranskat)abstract
- Biomarkers of neurodegenerative disorders are needed to assist in diagnosis, to monitor disease progression and therapeutic interventions, and to provide insight into disease mechanisms. One route to identify such biomarkers is by proteomic and peptidomic analysis of cerebrospinal fluid (CSF). In the current study, we performed an in-depth analysis of the human CSF endopeptidome to establish an inventory thatmay serve as a basis for future targeted biomarker studies. High-pH RP HPLC was employed for off-line sample prefractionation followed by low-pH nano-LC-MS analysis. Different software programs and scoring algorithms for peptide identification were employed and compared. A total of 18 031 endogenous peptides were identified at a FDR of 1%, increasing the number of known endogenous CSF peptides 10fold compared to previous studies. The peptides were derived from 2 053 proteins of which more than 60 have been linked to neurodegeneration. Notably, among the findings were six peptides derived from microtubule-associated protein tau, three of which span the diagnostically interesting threonine-181 (Tau-F isoform). Also, 213 peptides from amyloid precursor protein were identified, 58 of which were partially or completely within the sequence of amyloid beta 1-40/42, as well as 109 peptides from apolipoprotein E, spanning sequences that discriminate between the E2/E3/E4 isoforms of the protein.
|
|
| 44. |
- Hansson, Karl, 1985, et al.
(författare)
-
Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid
- 2017
-
Ingår i: Jove-Journal of Visualized Experiments. - : MyJove Corporation. - 1940-087X. ; :130
-
Tidskriftsartikel (refereegranskat)abstract
- This protocol describes a method developed to identify endogenous peptides in human cerebrospinal fluid (CSF). For this purpose, a previously developed method based on molecular weight cut-off (MWCO) filtration and mass spectrometric analysis was combined with an offline high-pH reverse phase HPLC pre-fractionation step. Secretion into CSF is the main pathway for removal of molecules shed by cells of the central nervous system. Thus, many processes in the central nervous system are reflected in the CSF, rendering it a valuable diagnostic fluid. CSF has a complex composition, containing proteins that span a concentration range of 8-9 orders of magnitude. Besides proteins, previous studies have also demonstrated the presence of a large number of endogenous peptides. While less extensively studied than proteins, these may also hold potential interest as biomarkers. Endogenous peptides were separated from the CSF protein content through MWCO filtration. By removing a majority of the protein content from the sample, it is possible to increase the sample volume studied and thereby also the total amount of the endogenous peptides. The complexity of the filtrated peptide mixture was addressed by including a reverse phase (RP) HPLC pre-fractionation step at alkaline pH prior to LC-MS analysis. The fractionation was combined with a simple concatenation scheme where 60 fractions were pooled into 12, analysis time consumption could thereby be reduced while still largely avoiding co-elution. Automated peptide identification was performed by using three different peptide/protein identification software programs and subsequently combining the results. The different programs were complementary rather than comparable with less than 15% of the identifications overlapped between the three.
|
|
| 45. |
- Hansson, Oskar, et al.
(författare)
-
Cerebral hypoperfusion is not associated with an increase in amyloid β pathology in middle-aged or elderly people
- 2018
-
Ingår i: Alzheimer's and Dementia. - : Wiley. - 1552-5279 .- 1552-5260. ; 14:1, s. 54-61
-
Tidskriftsartikel (refereegranskat)abstract
- INTRODUCTION: It is hypothesized that cerebral hypoperfusion promotes the development of Alzheimer pathology. We therefore studied whether longstanding cerebral hypoperfusion is associated with Alzheimer pathology in nondemented humans.METHODS: Cerebral blood flow and amyloid β ((18)F-Flutemetamol) positron emission tomography retention were assessed in eleven patients with unilateral occlusion of precerebral arteries resulting in chronic and uneven hypoperfusion. A subset of patients underwent tau ((18)F-AV-1451) positron emission tomography.RESULTS: The blood flow was significantly reduced on the affected side of the brain in patients with unilateral occlusion of the internal carotid artery or stenosis of the middle cerebral artery. However, the cortical uptake of (18)F-Flutemetamol or (18)F-AV-1451 was not altered.DISCUSSION: Our results suggest that longstanding cerebral hypoperfusion in humans does not result in accumulation of amyloid β fibrils or tau aggregates.
|
|
| 46. |
- Hansson, Oskar, et al.
(författare)
-
CSF biomarkers of Alzheimer's disease concord with amyloid-β PET and predict clinical progression : A study of fully automated immunoassays in BioFINDER and ADNI cohorts
- 2018
-
Ingår i: Alzheimer's and Dementia. - : Wiley. - 1552-5260 .- 1552-5279. ; 14:11, s. 1470-1481
-
Tidskriftsartikel (refereegranskat)abstract
- Introduction: We studied whether fully automated Elecsys cerebrospinal fluid (CSF) immunoassay results were concordant with positron emission tomography (PET) and predicted clinical progression, even with cutoffs established in an independent cohort. Methods: Cutoffs for Elecsys amyloid-β1–42 (Aβ), total tau/Aβ(1–42), and phosphorylated tau/Aβ(1–42) were defined against [18F]flutemetamol PET in Swedish BioFINDER (n = 277) and validated against [18F]florbetapir PET in Alzheimer's Disease Neuroimaging Initiative (n = 646). Clinical progression in patients with mild cognitive impairment (n = 619) was studied. Results: CSF total tau/Aβ(1–42) and phosphorylated tau/Aβ(1–42) ratios were highly concordant with PET classification in BioFINDER (overall percent agreement: 90%; area under the curve: 94%). The CSF biomarker statuses established by predefined cutoffs were highly concordant with PET classification in Alzheimer's Disease Neuroimaging Initiative (overall percent agreement: 89%–90%; area under the curves: 96%) and predicted greater 2-year clinical decline in patients with mild cognitive impairment. Strikingly, tau/Aβ ratios were as accurate as semiquantitative PET image assessment in predicting visual read–based outcomes. Discussion: Elecsys CSF biomarker assays may provide reliable alternatives to PET in Alzheimer's disease diagnosis.
|
|
| 47. |
- Hansson, Tobias, 1983, et al.
(författare)
-
Bichromatically pumped microresonator frequency combs
- 2014
-
Ingår i: Physical Review A - Atomic, Molecular, and Optical Physics. - 2469-9926 .- 2469-9934. ; 90:1
-
Tidskriftsartikel (refereegranskat)abstract
- A study is made of the nonlinear dynamics of bichromatically pumped microresonator Kerr frequency combs described by a driven and damped nonlinear Schrodinger equation, with an additional degree of freedom in the form of the modulation frequency. A truncated four-wave model is derived for the pump modes and the dominant sideband pair, which is found to be able to describe much of the essential dynamical behavior of the full equation. The stability of stationary states within the four-wave model is investigated, and numerical simulations are made to demonstrate that a large range of solutions, including cavity solitons, are possible beyond previously considered low-intensity patterns.
|
|
| 48. |
- Hansson, Tobias, 1983-, et al.
(författare)
-
Bistable solitons in third-harmonic generation frequency combs
- 2023
-
Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE.
-
Konferensbidrag (refereegranskat)abstract
- We present a theoretical model for describing dissipative solitons and optical frequency combs formation in a dispersive and nonlinear χ(3)-based cavity system that is phase-matched for third-harmonic generation. We consider the importance of the stability properties of the homogeneous solution in generating various types of multi-frequency combs, and demonstrate a novel type of bistable cavity solitons.
|
|
| 49. |
- Hansson, Tobias, 1983, et al.
(författare)
-
Complex temporal dynamics in optical cavities
- 2015
-
Ingår i: 2015 Spatiotemporal Complexity in Nonlinear Optics, SCNO 2015, Como, Italy, 31 August-4 September. - 9781467380614
-
Konferensbidrag (refereegranskat)abstract
- We overview recent advances in the modeling and understanding of complex spatiotemporal dynamics associated with the generation of optical frequency combs in microresonators.
|
|
| 50. |
|
|
