| 1. |
- Tabiri, S, et al.
(författare)
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- 2021
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swepub:Mat__t
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| 2. |
- Bravo, L, et al.
(författare)
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- 2021
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swepub:Mat__t
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| 3. |
- Hardt, Jochen, 1975, et al.
(författare)
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Health care utilization in primary care: Meaning of psychiatric disorders and adverse childhood experiences
- 2009
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Ingår i: Zeitschrift Fur Gesundheitspsychologie. - : Hogrefe Publishing Group. - 0943-8149 .- 2190-6289. ; 17:1
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Tidskriftsartikel (refereegranskat)abstract
- There is evidence that the use of the health care system with respect to somatic treatment and diagnoses is strongly influenced by the psychological determinants of the patients. The aim of this study is to analyze the effect of psychic comorbidities and childhood adversities on the usage of the primary health care system. A total of 453 patients were examined in the practices of their general practitioners with regard to childhood adversities. Data of 366 patients could be combined with the records of the practitioners about health care utilisation, and somatic and psychiatric diagnoses. A graphical Markov model was used to analyse the interdependencies, Psychiatric disorders influence the number of doctor visits and the time the practitioner spends with the patient. The latter was found to be the case, not only with regard to psychiatric but also to somatic treatment and diagnostic procedures aiming to discover somatic disease. There is no evidence that conversely somatic disorders increase psychiatric oriented diagnosis. Childhood adversities are not associated with any parameter of health care use that was examined. To optimize diagnostic and therapy of psychiatric and somatic dysfunctions and to avoid detentions, the strict separation of somatic medicine (first) and (then) psychiatric diagnostics and treatment should become Subject to discussion.
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| 8. |
- Hardt, U, et al.
(författare)
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Analysis of IGH allele content in a sample group of rheumatoid arthritis patients demonstrates unrevealed population heterogeneity
- 2023
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Ingår i: Frontiers in immunology. - : Frontiers Media SA. - 1664-3224. ; 14, s. 1073414-
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Tidskriftsartikel (refereegranskat)abstract
- Immunoglobulin heavy chain (IGH) germline gene variations influence the B cell receptor repertoire, with resulting biological consequences such as shaping our response to infections and altering disease susceptibilities. However, the lack of information on polymorphism frequencies in the IGH loci at the population level makes association studies challenging. Here, we genotyped a pilot group of 30 individuals with rheumatoid arthritis (RA) to examine IGH allele content and frequencies in this group. Eight novel IGHV alleles and one novel IGHJ allele were identified in the study. 15 cases were haplotypable using heterozygous IGHJ6 or IGHD anchors. One variant, IGHV4-34*01_S0742, was found in three out of 30 cases and included a single nucleotide change resulting in a non-canonical recombination signal sequence (RSS) heptamer. This variant allele, shown by haplotype analysis to be non-expressed, was also found in three out of 30 healthy controls and matched a single nucleotide polymorphism (SNP) described in the 1000 Genomes Project (1KGP) collection with frequencies that varied between population groups. Our finding of previously unreported alleles in a relatively small group of individuals with RA illustrates the need for baseline information about IG allelic frequencies in targeted study groups in preparation for future analysis of these genes in disease association studies.
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| 10. |
- Westermann, Alexander J., et al.
(författare)
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The Major RNA-Binding Protein ProQ Impacts Virulence Gene Expression in Salmonella enterica Serovar Typhimurium
- 2019
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Ingår i: mBio. - : American Society for Microbiology. - 2161-2129 .- 2150-7511. ; 10:1
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Tidskriftsartikel (refereegranskat)abstract
- FinO domain proteins such as ProQ of the model pathogen Salmonella enterica have emerged as a new class of major RNA-binding proteins in bacteria. ProQ has been shown to target hundreds of transcripts, including mRNAs from many virulence regions, but its role, if any, in bacterial pathogenesis has not been studied. Here, using a Dual RNA-seq approach to profile ProQ-dependent gene expression changes as Salmonella infects human cells, we reveal dysregulation of bacterial motility, chemotaxis, and virulence genes which is accompanied by altered MAPK (mitogen-activated protein kinase) signaling in the host. Comparison with the other major RNA chaperone in Salmonella, Hfq, reinforces the notion that these two global RNA-binding proteins work in parallel to ensure full virulence. Of newly discovered infection-associated ProQ-bound small noncoding RNAs (sRNAs), we show that the 3′UTR-derived sRNA STnc540 is capable of repressing an infection-induced magnesium transporter mRNA in a ProQ-dependent manner. Together, this comprehensive study uncovers the relevance of ProQ for Salmonella pathogenesis and highlights the importance of RNA-binding proteins in regulating bacterial virulence programs.IMPORTANCE The protein ProQ has recently been discovered as the centerpiece of a previously overlooked “third domain” of small RNA-mediated control of gene expression in bacteria. As in vitro work continues to reveal molecular mechanisms, it is also important to understand how ProQ affects the life cycle of bacterial pathogens as these pathogens infect eukaryotic cells. Here, we have determined how ProQ shapes Salmonella virulence and how the activities of this RNA-binding protein compare with those of Hfq, another central protein in RNA-based gene regulation in this and other bacteria. To this end, we apply global transcriptomics of pathogen and host cells during infection. In doing so, we reveal ProQ-dependent transcript changes in key virulence and host immune pathways. Moreover, we differentiate the roles of ProQ from those of Hfq during infection, for both coding and noncoding transcripts, and provide an important resource for those interested in ProQ-dependent small RNAs in enteric bacteria.
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