| 1. |
- Danielsson-Tham, Marie-Louise, 1947-, et al.
(författare)
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Causes behind a human cheese-borne outbreak of gastrointestinal listeriosis
- 2004
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Ingår i: Foodborne pathogens and disease. - New York, USA : Mary Ann Liebert. - 1535-3141 .- 1556-7125. ; 1:3, s. 153-159
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Tidskriftsartikel (refereegranskat)abstract
- In a previous paper, we reported an outbreak of gastrointestinal listeriosis due to consumption of fresh cheese made from raw milk and manufactured on a summer farm. The aim of the present study was to investigate why the cheese harbored Listeria monocytogenes. To our knowledge, this is the first documented outbreak of listeriosis caused by raw milk cheese where the human epidemic strain has been cultured from a dairy animal, whose milk has been used for cheese production. The conditions on a summer farm can hardly fulfil the requirements for hygienic and strictly controlled conditions necessary for safe processing of fresh cheese.
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| 2. |
- Ingermaa, Fredrika, et al.
(författare)
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Sheep, humans and listeriosis
- 2004
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Ingår i: ISOPOL XV. XV International Symposium on Problems of Listeriosis. Uppsala, Sweden, September 12–15, 2004. - Uppsala : Dep of Food Hygiene, Faculty of Veterinary Medicine. ; , s. Abstract no. 129-
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Konferensbidrag (refereegranskat)
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| 3. |
- Lindstedt, Bjørn-Arne, et al.
(författare)
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Multiple-locus variable-number tandem-repeats analysis of Listeria monocytogenes using multicolour capillary electrophoresis and comparison with pulsed-field gel electrophoresis typing
- 2008
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Ingår i: Journal of Microbiological Methods. - : Elsevier BV. - 0167-7012 .- 1872-8359. ; 72:2, s. 141-148
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Tidskriftsartikel (refereegranskat)abstract
- The multiple-locus variable-number tandem-repeats analysis (MLVA) method for genotyping has proven to be a fast and reliable typing tool in several bacterial species. MLVA is in our laboratory the routine typing method for Salmonella enterica subsp. enterica serovar Typhimurium and Escherichia coli O157. The gram-positive bacteria Listeria monocytogenes, while not isolated as frequent as S. Typhimurium and E. coli, causes severe illness with an overall mortality rate of 30%. Thus, it is important that any outbreak of this pathogen is detected early and a fast trace to the source can be performed. In view of this, we have used the information provided by two fully sequenced L. monocytogenes strains to develop a MLVA assay coupled with high-resolution capillary electrophoresis and compared it to pulsed-field gel electrophoresis (PFGE) in two sets of isolates, one Norwegian (79 isolates) and one Swedish (61 isolates) set. The MLVA assay could resolve all of the L. monocytogenes serotypes tested, and was slightly more discriminatory than PFGE for the Norwegian isolates (28 MLVA profiles and 24 PFGE profiles) and opposite for the Swedish isolates (42 MLVA profiles and 43 PFGE profiles).
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| 4. |
- Lopez-Valladares, Gloria, 1963-, et al.
(författare)
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Human isolates of Listeria monocytogenes in Sweden during half a century (1958-2010)
- 2014
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Ingår i: Epidemiology and Infection. - 0950-2688 .- 1469-4409. ; 142, s. 2251-2260
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Tidskriftsartikel (refereegranskat)abstract
- Isolates of Listeria monocytogenes (n=932) isolated in Sweden during 1958–2010 from human patients with invasive listeriosis were characterized by serotyping and pulsed-field gel electrophoresis (PFGE) (AscI). Of the 932 isolates, 183 different PFGE types were identified, of which 83 were each represented by only one isolate. In all, 483 serovar 1/2a isolates were distributed over 114 PFGE types; 90 serovar 1/2b isolates gave 32 PFGE types; 21 serovar 1/2c isolates gave nine PFGE types; three serovar 3b isolates gave one PFGE type; and, 335 serovar 4b isolates gave 31 PFGE types. During the 1980s in Sweden, several serovar 4b cases were associated with the consumption of European raw soft cheese. However, as cheese-production hygiene has improved, the number of 4b cases has decreased. Since 1996, serovar 1/2a has been the dominant L. monocytogenes serovar in human listeriosis in Sweden. Therefore, based on current serovars and PFGE types, an association between human cases of listeriosis and the consumption of vacuum-packed gravad and cold-smoked salmon is suggested.
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| 5. |
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| 6. |
- Parihar, Vishal Singh, et al.
(författare)
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Characterization of human invasive isolates of Listeria monocytogenes in Sweden 1986-2007
- 2008
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Ingår i: Foodborne pathogens and disease. - : Mary Ann Liebert. - 1535-3141 .- 1556-7125. ; 5:6, s. 755-761
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Tidskriftsartikel (refereegranskat)abstract
- Since 1986, 68% of the Listeria monocytogenes isolates from human cases of invasive listeriosis in Sweden are available for retrospective studies. The aim of the present study was to characterize 601 human invasive isolates of L. monocytogenes in Sweden from 1986 to 2007 by using serotyping and pulsed-field gel electrophoresis. Since 1996, serovar 4b was permanently reduced to the second or third most common serovar in human cases in Sweden. During the latter period, 2000-2007, only 13% belonged to serovar 4b and 71% to 1/2a. The dendrogram, based on pulsovars, reveals two clusters with different serovars. Cluster 1 exhibits serovars 4b and 1/2b, whereas cluster 2 consists of serovar 1/2a. Serovar 1/2a seems to be more heterogeneous than serovar 4b.
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| 7. |
- Parihar, Vishal, 1979-, et al.
(författare)
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Zoonotic aspects of Listeria monocytogenes
- 2004
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Ingår i: ISOPOL XV – XV International Symposium on Problems of Listeriosis, Uppsala, Sweden, September 12–15, 2004. - Uppsala. ; , s. Abstract no. 128-
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Konferensbidrag (refereegranskat)
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| 8. |
- Peiris, Inoka Priyadarshani, et al.
(författare)
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Gravad (Gravlax) and cold-smoked salmon, still a potential source of listeriosis
- 2009
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Ingår i: Journal of Foodservice. - Oxford : Blackwell Publishing. - 1748-0140 .- 1748-0159. ; 20:1, s. 15-20
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Tidskriftsartikel (refereegranskat)abstract
- Gravad (Gravlax) and cold-smoked salmon are associated with human listeriosis in Sweden. The present investigation of Listeria monocytogenes in salmon was a follow-up of a similar study in the middle of the 1990s. Since our first study, there has been an increasing focus on food hygiene in general and specially on self-inspection and Hazard Analysis and Critical Control Points from the authorities and food producers. L. monocytogenes was isolated from 11 (three manufacturers) of the 56 products analyzed. The highest level of L. monocytogenes was 1500 cfu/g from a cold-smoked salmon product; however, the level was low (<100 cfu/g) in most products. Serovar 1/2a was predominant, followed by 4b. restriction enzyme analysis/ pulsed-field gel electrophoresis typing of the 56 salmon isolates identified five types of L. monocytogenes. One type was identical to a human type, whereas two other were closely related. These findings suggest that gravad and coldsmoked salmon are still possible sources of listeriosis in Sweden.
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| 9. |
- Tham, Wilhelm, 1951-, et al.
(författare)
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A listeriosis patient infected with two different Listeria monocytogenes strains
- 2002
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Ingår i: Epidemiology and Infection. - 0950-2688 .- 1469-4409. ; 128:1, s. 105-106
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Tidskriftsartikel (refereegranskat)abstract
- Normally, only one isolate of Listeria monocytogenes from a case of listeriosis is subjected to characterization. Here we show that two isolates from different sites of the body were not the same strain. Such a phenomenon may not have any clinical relevance, although it may confuse the epidemiologist trying to match infection source with infection target.
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| 10. |
- Tham, Wilhem, 1951-, et al.
(författare)
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Occurrence of Genetic Variants of Listeria monocytogenes Strains
- 2013
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Ingår i: Foodborne pathogens and disease. - : Mary Ann Liebert. - 1535-3141 .- 1556-7125. ; 10:9, s. 825-826
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Tidskriftsartikel (refereegranskat)abstract
- Isolates of Listeria monocytogenes saved from outbreaks of listeriosis, cases of sporadic listeriosis, and similar events do not always belong to a solitary genetic variant. Variants of the same strain may have evolved from a unique clone, and plasmid loss or gain and phage-mediated genetic changes are suggested as the main mechanism. Some of these reports are summarized in this short communication.
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| 11. |
- Tham, Wilhelm, 1951-, et al.
(författare)
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Swedish human cases of Listeriosis, 1958–1999 : Visulized by LEGO Bricks
- 2000
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Ingår i: Nordic PFGE meeting January 27–28, 2000. Collection of abstracts. Department of Food Hygiene, Faculty of Veterinary Medicine, SLU, Uppsala. - Uppsala : Dep of Food Hygiene, Faculty of Veterinary Medicine, SLU. - 9157659621 ; , s. 27-27
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Konferensbidrag (refereegranskat)
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