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- Henriksson, Ida, 1990, et al.
(författare)
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Increased lipid accumulation and adipogenic gene expression of adipocytes in 3D bioprinted nanocellulose scaffolds
- 2017
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Ingår i: Biofabrication. - : IOP Publishing. - 1758-5082 .- 1758-5090. ; 9:1, s. Article Number: 015022 -
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Tidskriftsartikel (refereegranskat)abstract
- Compared to standard 2D culture systems, new methods for 3D cell culture of adipocytes could provide more physiologically accurate data and a deeper understanding of metabolic diseases such as diabetes. By resuspending living cells in a bioink of nanocellulose and hyaluronic acid, we were able to print 3D scaffolds with uniform cell distribution. After one week in culture, cell viability was 95%, and after two weeks the cells displayed a more mature phenotype with larger lipid droplets than standard 2D cultured cells. Unlike cells in 2D culture, the 3D bioprinted cells did not detach upon lipid accumulation. After two weeks, the gene expression of the adipogenic marker genes PPAR. and FABP4 was increased 2.0- and 2.2-fold, respectively, for cells in 3D bioprinted constructs compared with 2D cultured cells. Our 3D bioprinted culture system produces better adipogenic differentiation of mesenchymal stem cells and a more mature cell phenotype than conventional
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- Håkansson, Karl, 1984, et al.
(författare)
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Solidification of 3D printed nanofibril hydrogels into functional 3D cellulose structures
- 2016
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Ingår i: Advanced Materials Technologies. - : Wiley. - 2365-709X. ; 1:7, s. 1600096-
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Tidskriftsartikel (refereegranskat)abstract
- Cellulose nanofibrils isolated from trees have the potential to be used as raw material for future sustainable products within the areas of packaging, textiles, biomedical devices, and furniture. However, one unsolved problem has been to convert the nanofibril-hydrogel into a dry 3D structure. In this study, 3D printing is used to convert a cellulose nanofibril hydrogel into 3D structures with controlled architectures. Such structures collapse upon drying, but by using different drying processes the collapse can be controlled and the 3D structure can be preserved upon solidification. In addition, a conductive cellulose nanofibril ink is fabricated by adding carbon nanotubes. These findings enable the use of wood derived materials in 3D printing for fabrication of sustainable commodities such as packaging, textiles, biomedical devices, and furniture with conductive parts. Furthermore, with the introduction of biopolymers into 3D printing, the 3D printing technology itself can finally be regarded as sustainable.
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