| 1. |
- Arup, Ulf, et al.
(författare)
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Lavar – Lichens
- 2010
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Ingår i: Rödlistade arter i Sverige 2010 – The 2010 Red List of Swedish Species. - 9789188506351 ; , s. 285-300
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 2. |
- Ahlsson, Anders, 1962-, et al.
(författare)
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A Swedish consensus on the surgical treatment of concomitant atrial fibrillation
- 2012
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Ingår i: Scandinavian Cardiovascular Journal. - London, United Kingdom : Informa Healthcare. - 1401-7431 .- 1651-2006. ; 46:4, s. 212-218
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Forskningsöversikt (refereegranskat)abstract
- Atrial fibrillation (AF) is a common arrhythmia among patients scheduled for open heart surgery and is associated with increased morbidity and mortality. According to international guidelines, symptomatic and selected asymptomatic patients should be offered concomitant surgical AF ablation in conjunction with valvular or coronary surgery. The gold standard in AF surgery is the Cox Maze III ("cut-and-sew") procedure, with surgical incisions in both atria according to a specified pattern, in order to prevent AF reentry circuits from developing. Over 90% of patients treated with the Cox Maze III procedure are free of AF after 1 year. Recent developments in ablation technology have introduced several energy sources capable of creating nonconducting atrial wall lesions. In addition, simplified lesion patterns have been suggested, but results with these techniques have been unsatisfactory. There is a clear need for standardization in AF surgery. The Swedish Arrhythmia Surgery Group, represented by surgeons from all Swedish units for cardiothoracic surgery, has therefore reached a consensus on surgical treatment of concomitant AF. This consensus emphasizes adherence to the lesion pattern in the Cox Maze III procedure and the use of biatrial lesions in nonparoxysmal AF.
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| 3. |
- Arup, Ulf, et al.
(författare)
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Lavar Lichenes
- 2015
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Ingår i: Rödlistade arter i Sverige 2015. - 9789187853104 ; , s. 72-79
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 4. |
- Arup, Ulf, et al.
(författare)
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Rödlista över lavar Lichenes
- 2020
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Ingår i: Rödlistade arter i Sverige 2020. - 9789187853548 - 9789187853555 ; , s. 89-96
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 5. |
- Bornefalk Hermansson, Anna, 1971-
(författare)
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Resampling Evaluation of Signal Detection and Classification : With Special Reference to Breast Cancer, Computer-Aided Detection and the Free-Response Approach
- 2007
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The first part of this thesis is concerned with trend modelling of breast cancer mortality rates. By using an age-period-cohort model, the relative contributions of period and cohort effects are evaluated once the unquestionable existence of the age effect is controlled for. The result of such a modelling gives indications in the search for explanatory factors. While this type of modelling is usually performed with 5-year period intervals, the use of 1-year period data, as in Paper I, may be more appropriate.The main theme of the thesis is the evaluation of the ability to detect signals in x-ray images of breasts. Early detection is the most important tool to achieve a reduction in breast cancer mortality rates, and computer-aided detection systems can be an aid for the radiologist in the diagnosing process.The evaluation of computer-aided detection systems includes the estimation of distributions. One way of obtaining estimates of distributions when no assumptions are at hand is kernel density estimation, or the adaptive version thereof that smoothes to a greater extent in the tails of the distribution, thereby reducing spurious effects caused by outliers. The technique is described in the context of econometrics in Paper II and then applied together with the bootstrap in the breast cancer research area in Papers III-V.Here, estimates of the sampling distributions of different parameters are used in a new model for free-response receiver operating characteristic (FROC) curve analysis. Compared to earlier work in the field, this model benefits from the advantage of not assuming independence of detections in the images, and in particular, from the incorporation of the sampling distribution of the system's operating point.Confidence intervals obtained from the proposed model with different approaches with respect to the estimation of the distributions and the confidence interval extraction methods are compared in terms of coverage and length of the intervals by simulations of lifelike data.
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| 6. |
- Buskqvist, Ulf, 1975-, et al.
(författare)
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Inbetween Literacy Desirings and Following Commands : Rethinking Digitalization in Swedish Early Childhood Education
- 2023
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Ingår i: Nordic Journal of Studies in Educational Policy. - : Taylor & Francis. - 2002-0317. ; 9:2, s. 210-220
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Tidskriftsartikel (refereegranskat)abstract
- This article is an empirically grounded contribution to the understanding of how digitalization in education is interpreted and made into being by actors in everyday lives, in this case children and teachers in Swedish early childhood education. The focus is on interactions in an early childhood classroom upon and around a digital interactive floor setting. Drawing on the theoretical concept of socio-spatiality, this literacy event is understood as an enactment of policy where both teacher and children become actors and subjects. Using observations and video recordings as a methodological approach, the study shows how the literacy event in the digital setting is enacted and constituted as a relational process between literacy desirings and education policy regarding digitalization. The authors suggest that engaging with both children’s processes of literacy desirings and educational commands in ECE digital activities serves as a productive way to investigate how digitalization is enacted in contemporary ECE.
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| 7. |
- Dyverfeldt, Petter, et al.
(författare)
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Hemodynamic aspects of mitral regurgitation assessed by generalized phase-contrast MRI
- 2011
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Ingår i: Journal of Magnetic Resonance Imaging. - : John Wiley and Sons. - 1053-1807 .- 1522-2586. ; 33:3, s. 582-588
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: Mitral regurgitation creates a high velocity jet into the left atrium (LA), contributing both volume andpressure; we hypothesized that the severity of regurgitation would be reflected in the degree of LA flowdistortion.Material and Methods: Three-dimensional cine PC-MRI was applied to determine LA flow patterns andturbulent kinetic energy (TKE) in seven subjects (five patients with posterior mitral leaflet prolapse, two normalsubjects). In addition, the regurgitant volume and the time-velocity profiles in the pulmonary veins weremeasured.Results: The LA flow in the mitral regurgitation patients was highly disturbed with elevated values of TKE.Peak TKE occurred consistently at late systole. The total LA TKE was closely related to the regurgitant volume.LA flow patterns were characterized by a pronounced vortex in proximity to the regurgitant jet. In some patients,pronounced discordances were observed between individual pulmonary venous inflows, but these could not berelated to the direction of the flow jet or parameters describing global LA hemodynamics.Conclusion: PC-MRI permits investigations of atrial and pulmonary vein flow patterns and TKE in significantmitral regurgitation, reflecting the impact of the highly disturbed blood flow that accompanies this importantvalve disease.
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| 8. |
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| 9. |
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| 10. |
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| 11. |
- Escobar Kvitting, John-Peder, et al.
(författare)
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Resection of a plasma cell granuloma combining a conventional posterolateral left-sided thoracotomy with a minimally invasive valve approach
- 2019
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Ingår i: General Thoracic and Cardiovascular Surgery. - : SPRINGER JAPAN KK. - 1863-6705 .- 1863-6713. ; 67:10, s. 894-896
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Tidskriftsartikel (refereegranskat)abstract
- Plasma cell granuloma (PCG) is a rare benign tumor that is difficult to differentiate from malignancy. Depending on the location of the PCG, surgical management can be challenging. We describe a patient with a PCG involving the left lower lobe extending into the left atrium, that was resected en bloc using a conventional posterolateral thoracotomy combined with a surgical approach predominantly used for minimally invasive mitral valve surgery. This case illustrates how it is possible to utilize a technique used for cardiac surgery for tumors of pulmonary origin involving the heart.
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| 12. |
- Gisterå, A., et al.
(författare)
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Vaccination against T-cell epitopes of native ApoB100 reduces vascular inflammation and disease in a humanized mouse model of atherosclerosis
- 2017
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Ingår i: Journal of Internal Medicine. - : Wiley. - 0954-6820 .- 1365-2796. ; 281:4, s. 383-397
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Tidskriftsartikel (refereegranskat)abstract
- Background and Objectives: The T-cell response to low-density lipoprotein (LDL) in the vessel wall plays a critical role in atherosclerotic plaque formation and stability. In this study, we used a new translational approach to investigate epitopes from human apolipoprotein B100 (ApoB100), the protein component of LDL, which triggers T-cell activation. We also evaluated the potential of two selected native ApoB100 epitopes to modulate atherosclerosis in human ApoB100-transgenic Ldlr-/- (HuBL) mice. Methods and Results: HuBL mice were immunized with human atherosclerotic plaque homogenate to boost cellular autoimmune response to tissue-derived ApoB100 epitopes. In vitro challenge of splenocytes from immunized mice with a library of overlapping native peptides covering human ApoB100 revealed several sequences eliciting T-cell proliferation. Of these sequences, peptide (P) 265 and P295 were predicted to bind several human leucocyte antigen (HLA) haplotypes and induced high levels of interferon (IFN)-γ. Vaccination of HuBL mice with these peptides mounted a strong adaptive immune response to native ApoB100, including high levels of epitope-specific plasma IgGs. Interestingly, P265 and P295 vaccines significantly decreased plaque size, reduced macrophage infiltration and increased IgG1 deposition in the plaques. Purified IgGs from vaccinated mice displayed anti-inflammatory properties against macrophages in vitro, reducing their response to LPS in a dose-dependent manner. Conclusion: We identified two specific epitopes from human native ApoB100 that trigger T-cell activation and protect HuBL mice against atherosclerosis when used in a vaccine. Our data suggest that vaccination-induced protective mechanisms may be mediated at least in part through specific antibody responses to LDL that inhibit macrophage activation.
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| 13. |
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| 14. |
- Helldahl, Per, 1980-
(författare)
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The Challenge from Nationalism : Problems of Community in Democracy
- 2013
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The dissertation examines the relationship between democracy and nationalism from a normative standpoint. A point of departure is the assumption that any democracy requires a referent community, or demos. Nationalism has, in practice, frequently provided democracies with this sense of community during the last two centuries. The author argues, firstly, that this connection has led to an entanglement of the concepts of democracy and nationalism, so that democrats tend to rely, often unknowingly, on the thought structures of nationalism as they seek to make explicit the identity of their respective communities. The mechanism by which this connection is upheld is demonstrated through two contextualized studies of discourse on common society-wide identity in, respectively, the contemporary United States of America and the contemporary Federal Republic of Germany. Secondly, it is argued (also on the basis of these contextualized studies) that the nationalist features which tend to ‘leak’ into the overarching, society-wide identities that are constructed in these debates contain an inherently exclusionary potential; however, this leakage is often glossed over by superficial anti-nationalism and phrases such as ‘civic nationalism’, which is contrasted with ‘ethnic nationalism’. Rather than hidden behind such rhetoric, the author argues, the nationalist thought structures that democrats tend to rely on should be brought into the light of day, so that the potentially destructive features of nationalism can be handled in the best way possible. Thirdly, it is claimed that deliberative models (such as that of Jürgen Habermas) are better suited than liberal nationalist models (such as that of David Miller) for this task.
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| 15. |
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| 16. |
- Hermansson-Borrebaeck, Rasmus, et al.
(författare)
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Beliefs about medications when treating hypertension in primary health care : results from "PERson-centredness in hypertension management using information Technology (PERHIT)"
- 2023
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Ingår i: Blood Pressure. - 0803-7051. ; 32:1
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Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: Hypertension is a major global health concern. Despite of efficient antihypertensive medications a low percentage of patients reach a blood pressure (BP) of <140/90. Nonadherence is a great concern in hypertension treatment and patients' beliefs about medications has been shown to have a strong impact on adherence. The objective of this study is to examine beliefs about medications and its impact on BP treatment in a group of Swedish primary healthcare patients treated for hypertension with or without an E-health platform.MATERIALS AND METHOD: In a randomised unblinded controlled trial, 949 patients with hypertension from Swedish primary health care centres were included. The intervention group used a web-based system to support self-management of hypertension for eight weeks. Beliefs about medication questionnaire (BMQ) were administered to all patients at inclusion, 8-week follow up and 1-year follow up.RESULTS: Data were collected from the 862 patients who completed the trial. No statistically significant difference was found in BMQ-scores between the intervention and the control group. An association between lower scores in the BMQ subsection 'General-Harm' and achieving target BP of <140/90 mmHg were noted ( p = 0.021). CONCLUSION: This study shows a significant association between beliefs about medication and BP levels, on hypertensive patients in the Swedish primary care setting, in only one out of four subsections of the BMQ. The intervention did not have a significant effect on changing patients' beliefs about medication. Further emphasis on patients' beliefs about medications could be useful in the clinical setting.
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| 17. |
- Hermansson, Christoffer, 1975, et al.
(författare)
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Artificiell intelligens och juristens kompetens
- 2021
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Ingår i: Noll, G. (Red.) (2021). AI, digitalisering och rätten : en lärobok. Studentlitteratur. - Lund : Studentlitteratur. - 9789144137858 ; , s. 159-201
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 18. |
- Hermansson, Karin, et al.
(författare)
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Ultra-clean Si/Si interface formation by surface preparation and direct bonding in ultra-high vacuum
- 1998
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Ingår i: Proceedings of the Fourth International Symposium on Semiconductor Wafer Bonding: Science, Technology, and Applications. ; , s. 401-
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Konferensbidrag (refereegranskat)abstract
- Silicon surfaces have been cleaned and bonded in ultra-high vacuum at a pressure in the 10-10 torr range. The bonded interfaces show extremely low contamination levels as measured by secondary ion mass spectroscopy. Nevertheless, a potential barrier could be detected at the interface spreading resistance and current vs. temperature measurements. This suggests that the barrier is caused by inevitable dislocation networks due to wafer misorientation, as well as residual oxygen at the interface
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| 19. |
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| 20. |
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| 21. |
- Hermansson, Ruth S., et al.
(författare)
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Incidence of oncogenic HPV and HPV-related dysplasia five years after a negative HPV test by self-sampling in elderly women
- 2022
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Ingår i: Infectious Agents and Cancer. - : Springer Nature. - 1750-9378. ; 17:42
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: Cervical cancer prevention for older women can be challenging since there are no specific guidelines for this group. This study aimed to determine the incidence of oncogenic HPV and HPV-related dysplasia in elderly women 5 years after being HPV negative.Methods: Invited women participated five years earlier in a study where self-sampling for HPV testing was applied, at this time, they were all HPV negative. The women were now, five years later invited to perform self-sampling for HPV testing. Women with a positive result performed a repeat HPV test. Those with a positive repeat HPV test were examined by colposcopy, biopsy and cytology.Results: Of the 804 invited women, 634 (76.9%) agreed to participate in the study and a self-sampling kit was sent to them. Of these, 99.6% (632/634) sent a sample to the HPV laboratory. The participation rate in each age group was 93.3% at age 65, 74.0% at age 70, 80.7% at age 75 and 64.6% at age 80. Overall 18 women (2.8%, 95% CI 3.2 to 6.0) were HPV positive in the first test and 8 (1.3%, 95% CI 0.6 to 2.6) in the second test. Sampling for the second test was done on average 5.4 months after the first test. Fifty per cent (4/8) of the women with a positive repeat test had dysplasia in histology.Conclusions: The incidence of HPV in previously HPV-negative elderly women was low. Among women who were HPV positive in a repeat test, there was a high prevalence of low grade dysplasia.
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| 22. |
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| 23. |
- Hermansson, Veronica, et al.
(författare)
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Pharmacokinetics of the adrenocorticolytic compounds 3-methylsulphonyl-DDE and o,p′-DDD (mitotane) in Minipigs
- 2008
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Ingår i: Cancer Chemotherapy and Pharmacology. - : Springer Science and Business Media LLC. - 0344-5704 .- 1432-0843. ; 61:2, s. 267-274
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Tidskriftsartikel (refereegranskat)abstract
- The pharmacokinetics of the adrenocorticolytic drug candidate 3-Methylsulphonyl-DDE (3-MeSO2-DDE) and the anticancer drug o,p′-DDD (mitotane) were studied in Göttingen minipigs. The animals were given 3-MeSO2-DDE or o,p′-DDD as single oral doses (30 mg/kg). Concentrations in plasma and subcutaneous fat were measured by gas chromatography at different time points during 180 days. Maximal plasma concentrations appeared within 24 h for both compounds, but were about 2 times higher for 3-MeSO2-DDE. o,p′-DDD plasma concentrations declined rapidly to low levels during 4 days. 3-MeSO2-DDE also decreased rapidly, but remained at high concentrations throughout the study. In fat, 3-MeSO2-DDE reached about 25-fold higher levels than o,p′-DDD at 30 days, and both substances were eliminated slowly from this tissue. 3-MeSO2-DDE liver concentrations were about 18-fold higher than those in plasma at 180 days. In contrast, o,p′-DDD liver and plasma levels were about equal at 180 days. o,p′-DDD had roughly 45 times larger CL/F than 3-MeSO2-DDE, confirming that the elimination of this compound was more rapid. Both compounds were characterised by their localisation and retention in fat tissue, and the individual size of the fat stores clearly determined the plasma concentrations. It is concluded that although 3-MeSO 2-DDE is an interesting candidate for therapeutic use due to its potential characteristics to specifically target adrenocortical tumour cells the slow elimination of the compound might make it challenging to design appropriate dosage regimes.
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| 24. |
- Jönsson, Anders, et al.
(författare)
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Microwave Ablation in Mitral Valve Surgery for Atrial Fibrillation (MAMA)
- 2012
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Ingår i: Journal of Atrial Fibrillation. - : Cardiofront. - 1941-6911. ; 5:2, s. 13-22
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Tidskriftsartikel (refereegranskat)abstract
- Objective: Microwave ablation in conjunction with open heart surgery is effective in restoring sinus rhythm (SR) in patients with atrial fibrillation (AF). In patients assigned for isolated mitral valve surgery no prospective randomized trial has reported its efficacy.Methods: 70 patients with longlasting AF where included from 5 different centres. They were randomly assigned to mitral valve surgery and atrial microwave ablation or mitral valve surgery alone.Results: Out of 70 randomized, 66 and 64 patients were available for evaluation at 6 and 12 months. At 12 months SR was restored and preserved in 71.0 % in the ablation group vs 36.4 % in the control group (P=0.006), corresponding figures at 6 months was 62.5 % vs 26.5 % (P=0.003). The 30-day mortality rate was 1.4 %, with one death in the ablation group vs zero deaths in the control group. At 12 months the mortality rate was 7,1 % (Ablation n=3 vs Control n=2). No significant differences existed between the groups with regard to the overall rate of serious adverse events (SAE) during the perioperative period or at the end of the study. 16 % of patients randomized to ablation were on antiarrhytmic drugs compared to 6 % in the control group after 1 year (p=0.22).Conclusion: Microwave ablation of left and right atrium in conjunction with mitral valve surgery is safe and effectively restores sinus rhythm in patients with longlasting AF as compared to mitral valve surgery alone.
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| 25. |
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| 26. |
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| 27. |
- Lindström, Annika K., 1953-, et al.
(författare)
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Cervical dysplasia in elderly women performing repeated self-sampling for HPV testing
- 2018
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Ingår i: PLOS ONE. - : Public Library of Science. - 1932-6203. ; 13:12
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: About 30% of the cervical cancer cases in Sweden occur in women older than 60. The primary aim was to evaluate the acceptability of repeated self-sampling at home for HPV-testing in elderly women. The prevalence of HPV and HPV related dysplasia as well as the sensitivity of cytology was evaluated.METHODS: Repeated self-sampling at home for HPV testing was offered 375 women in each of the four age groups 60, 65, 70 and 75 years. Women with two consecutive positive HPV tests were examined with sampling for histology and cytology.FINDINGS: A self-sample was provided by 59.5% (893/1500) of the invited women. The overall prevalence of HPV was 4.4% (95% CI 3.2-6.0, n = 39) in the first test, and 2.5% were persistent positive in the second test (95% C 1.6-3.8, n = 22) collected on average 5.5 months later. Dysplasia, was found in 1.8% (16/893) (95% CI 1.1-3.0) and CIN 2+ in 1.0% (9/893) (95%CI 0.5-2.0) of the women. Of the 16 women with dysplasia in histology, 13 (81.2%) had a normal cytology.INTERPRETATION: Repeated self-sampling at home combined with HPV testing was well accepted among elderly women. A high prevalence of CIN was diagnosed by histology. Cytology showed extremely low sensitivity and should not be recommended for this age group.
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| 28. |
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| 29. |
- Löf, Liza, et al.
(författare)
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Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia
- 2017
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Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 7:1
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Tidskriftsartikel (refereegranskat)abstract
- Chronic myeloid leukemia (CML) is characterized in the majority of cases by a t(9;22)(q34;q11) translocation, also called the Philadelphia chromosome, giving rise to the BCR-ABL1 fusion protein. Current treatment with tyrosine kinase inhibitors is directed against the constitutively active ABL1 domain of the fusion protein, and minimal residual disease (MRD) after therapy is monitored by real-time quantitative PCR (RQ-PCR) of the fusion transcript. Here, we describe a novel approach to detect and enumerate cells positive for the BCR-ABL1 fusion protein by combining the in situ proximity ligation assay with flow cytometry as readout (PLA-flow). By targeting of the BCR and ABL1 parts of the fusion protein with one antibody each, and creating strong fluorescent signals through rolling circle amplification, PLA-flow allowed sensitive detection of cells positive for the BCR-ABL1 fusion at frequencies as low as one in 10,000. Importantly, the flow cytometric results correlated strongly to those of RQ-PCR, both in diagnostic testing and for MRD measurements over time. In summary, we believe this flow cytometry-based method can serve as an attractive approach for routine measurement of cells harboring BCR-ABL1 fusions, also allowing simultaneously assessment of other cell surface markers as well as sensitive longitudinal follow-up.
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| 30. |
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| 31. |
- Petäjä, Ulf, 1967-
(författare)
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Varför yttrandefrihet? : Om rättfärdigandet av yttrandefrihet med utgångspunkt från fem centrala argument i den demokratiska idétraditionen
- 2006
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis focuses primarily on the question ”why is freedom of speech valuable in a democratic context?” I argue that it is problematic that free-dom of speech takes for granted and that the main question therefore is absent in current political science research, in legal texts, and in public discourse. I also argue that in democratic states the focus, regarding freedom of speech, is often on its boundaries and limits rather than on its justification. But it is highly problematic to find and establish its limits without dis-cussion why freedom of speech is desirable in the first place.The thesis poses two questions. The first concerns how freedom of speech is justified by the five strongest available arguments. I analyze the arguments and conclude that they justify freedom of speech differently but that they are similar in one aspect. Freedom of speech is not primarily justified as an individual right. It is rather justified in terms of the public good.The second question asks if we can reach a better understanding of the central arguments. I argue that the arguments have something in common; all of them justify freedom of speech with reference to a common value. I argue that this common value is what I call, a “reliable communication process”. All five arguments claim that freedom of speech is valuable because it promotes a reliable communication process. This process is reliable in terms of its capacity to create a pluralistic public discourse that exposes citizens to ideas and perspectives that they would not have chosen in advance.This study results in the following findings. First, that freedom of speech is valuable in a democratic context because the reliable communication process supports the central democratic value of the enlightened understanding of the democratic citizen. Secondly, that I can give a principled reason for the boundaries of freedom of speech. This means that, according to the arguments, there are reasons to abolish or limit freedom of speech if the reliable communication process is damaged or absent, for example in case of war, anarchy, or violent circumstances. Third, that there are strong reasons in support of a public service media, and greater state intervention in media politics. One strong reason for that conclusion is that a public service media can ensure a pluralistic communication in society and counteract information conformity and intolerance among the members of society.
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| 32. |
- Simonsson, Martin, et al.
(författare)
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Epidermal keratinocyte loss in response to daily 2 Gy fractions for 5 weeks of radiotherapy is associated with DSB-foci, growth arrest, apoptosis and lack of accelerated repopulation
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Background and purpose: Tissue-sparing due to repopulation is expected to occur in epithelial cell populations during a course of fractionated radiotherapy (RT). Recently, we established, in the clinical setting of RT, the dose response relationship of subtherapeutic doses in terms of epidermal keratinocyte loss in the basal layer throughout 7 weeks of RT. Surprisingly, in the case of daily dose fractions of 1.10Gy, the keratinocyte loss per dose unit increases over the last 4 weeks of the treatment period rather than being constant or decreasing. The aim of the present study is to elaborate on the issue of germinal keratinocyte response to daily dose fractions of 2.0Gy for 5 weeks. Here, we present assessments of keratinocyte loss, DSB foci, growth arrest, mitosis and apoptosis using methods earlier described by us. Materials and Methods: In total 240 skin punch biopsies, collected from 31 breast cancer patients, before, during and after postmastectomy radiotherapy given to the thoracic wall with daily 2.0Gy fractions for 5 weeks, were investigated. The dose response for basal keratinocyte density of the interfollicular epidermis was determined. The DNA damage response of keratinocytes was studied by immunostaining for molecular markers of DNA DSBs, growth arrest, mitosis and cell death using 53BP1, p21, phospho-H3 and γH2AX (apoptosis), respectively. The stainings of keratinocytes were counted manually or by digital image analysis. Results: The dose-response relationship for the loss of basal keratinocytes over 5 weeks of RT revealed a biphasic shape. An initial radioresistant phase was followed by an increase in radiosensitivity in the second part of RT. The rate of keratinocyte loss reflected the significant changes determined by 53BP1 and γH2AX foci 30 minutes after dose fractions over the treatment period. The highest induction of DSB foci per cell was observed towards the end of treatment. The increase in the fraction of p21 stained cells was also more prominent during the second half of the treatment as compared to the first period of RT. The apoptotic frequency was generally low but increased dramatically towards the end of RT. The mitotic cell number was significantly suppressed over 5 weeks, and did not recover during the weekend treatment-gaps. Notably, the mitotic rate increased more than threefold compared to unexposed skin, 2 weeks after the end of RT, followed by a rapid decline one week later. Conclusion: The dose response for germinal keratinocyte loss as a result of daily dose fractions of 2.0Gy over 5 weeks treatment deviates significantly from an exponential curve fit. The effectiveness of each dose fraction was less in the first half of the treatment when compared with the second half. No accelerated repopulation could be revealed over the 5 weeks, but was evident after completion of radiotherapy. The changes in keratinocyte response were associated with changes in induction of DSB foci and p21 protein expression, as well as apoptotic events over the treatment period. In particular, we highlight the existence of pre-mitotic apoptosis, which increased significantly towards the end of 5 weeks RT. These findings suggest that it is necessary to reconsider the current conceptions regarding DNA repair, cell-cycle redistribution and repopulation of normal epithelial cells to a long-lasting courses of fractionated radiotherapy.
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| 33. |
- Szabó, Zoltán, et al.
(författare)
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Kiegészítő klinikai módszer a nyitott szívműtéteknél fellépő légembolisatio csökkentésére : [A complementary clinical method to minimize air embolism during open-heart surgery]
- 2008
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Ingår i: Magyar Sebeszet. - : Akademiai Kiado. - 0025-0295 .- 1789-4301. ; 61, s. 57-59
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Tidskriftsartikel (refereegranskat)abstract
- Air from the left heart is ejected even up to several hours after cardiopulmonary bypass (CPB) despite the use of CO 2 . The following method is complementary in addition to surgical de-airing in order to further reduce the chance of air embolism, especially from the pulmonary veins. After re-expanding the lungs with standard bag inflation, the ventilation is restarted in consultation with the surgeon. The ventilator is set to the respiratory minute volume used before the CPB but at a respiratory frequency of 10/minutes whereas the regularly beating heart is filled from the heart lung machine. Transoesophageal echocardiography (TEE) reliably controls the effect.
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| 34. |
- Thor, Göran, et al.
(författare)
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Blylav återfunnen i Padjelanta nationalpark efter 79 år
- 2021
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Ingår i: Fauna och Flora: populär tidskrift för biologi. - 0014-8903. ; 116:2, s. 14-17
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Padjelanta nationalpark har en exceptionellt artrik och fort farande till stor del okänd lavflora inklusive många sällsynta eller rödlistade arter. Lavforskaren Gunnar Degelius hittade 1941 den rödlistade blylaven på ett klippblock vid fjället Unna Duvgge på sydvästra stranden av Virihaure vilket är den nordligaste lokalen för arten i Sverige. Sommaren 2020 återfann vi blylaven efter 79 långa år, med stor sannolikhet på samma stenblock men totalt fanns det bara 18 cm2.
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| 35. |
- Thor, Göran, et al.
(författare)
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Eftersök av falsk guldskivlav Psora testacea på fastlandet och lavar på Fridö, Södermanland
- 2018
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Ingår i: Lavbulletinen. - 1651-6435. ; 2018:2, s. 66-77
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Fyra medlemmar i expertkommittén för lavar besökte 26 april 2018 Fridö och Furholmens naturreservat i närheten av Tullgarns slott. Syftet var att klargöra statusen för falsk guldskivlav Psora testacea, som rapporterades från Furholmen 1895, vilket är det enda rapporterade fyndet av arten på fastlandet. Arten hittades inte och då det inte heller finns något belägg är vårt förslag att rapporten av denna art från Södermanland är alltför osäker för att kunna valideras. En artlista inkluderande de arter som noterades från Fridö presenteras. Elva arter rapporteras som nya för Södermanland, Briancoppinsia cytospora, Calogaya arnoldii, Catillaria cryptophila,Endocarpon pusillum, Gyalidea subscutellaris, Muellerella erratica, Rinodina lecanorina, Scytinium aragonii, Verrucaria dolosa, V. macrostoma och V. tectorum.
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| 36. |
- Thor, Göran, et al.
(författare)
-
Lavar på Fridö, Södermanland, del 2
- 2019
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Ingår i: Lavbulletinen. - 1651-6435. ; 2019:2, s. 68-69
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- En artlista inkluderande de arter som noterades vid en exkursion till Fridö i Södermanland 2018 presenterades tidigare i Lavbulletinen. Några arter var då ännu inte bestämda eller bara preliminärt bestämda. Här kompletteras artlistan med Bacidina egenula (ny för Södermanland) och Rinodina calcarea (första fyndet utanför Öland och Gotland) vilken nu är konfirmerad genom DNA-analys.
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| 37. |
- Turesson, Ingela, et al.
(författare)
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Epidermal Keratinocyte Depletion during Five Weeks of Radiotherapy is Associated with DNA Double-Strand Break Foci, Cell Growth Arrest and Apoptosis: Evidence of Increasing Radioresponsiveness and Lack of Repopulation; The Number of Melanocytes Remains Unchanged
- 2020
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Ingår i: Radiation Research. - 0033-7587 .- 1938-5404. ; 193:5, s. 481-496
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Tidskriftsartikel (refereegranskat)abstract
- During fractionated radiotherapy, epithelial cell populations are thought to decrease initially, followed by accelerated repopulation to compensate cell loss. However, previous findings in skin with daily 1.1 Gy dose fractions indicate continued and increasing cell depletion. Here we investigated epidermal keratinocyte response with daily 2 Gy fractions as well as accelerated and hypofractionation. Epidermal interfollicular melanocytes were also assessed. Skin-punch biopsies were collected from breast cancer patients before, during and after mastectomy radiotherapy to the thoracic wall with daily 2 Gy fractions for 5 weeks. In addition, 2.4 Gy radiotherapy four times per week and 4 Gy fractions twice per week for 5 weeks, and two times 2 Gy daily for 2.5 weeks, were used. Basal keratinocyte density of the interfollicular epidermis was determined and immunostainings of keratinocytes for DNA double-strand break (DSB) foci, growth arrest, apoptosis and mitosis were quantified. In addition, interfollicular melanocytes were counted. Initially minimal keratinocyte loss was observed followed by pronounced depletion during the second half of treatment and full recovery at 2 weeks post treatment. DSB foci per cell peaked towards the end of treatment. p21-stained cell counts increased during radiotherapy, especially the second half. Apoptotic frequency was low throughout radiotherapy but increased at treatment end. Mitotic cell count was significantly suppressed throughout radiotherapy and did not recover during weekend treatment gaps, but increased more than threefold compared to unexposed skin 2 weeks post-radiotherapy. The number of melanocytes remained constant over the study period. Germinal keratinocyte loss rate increased gradually during daily 2 Gy fractions for 5 weeks, and similarly for hypofractionation. DSB foci number after 2 Gy irradiation revealed an initial radioresistance followed by increasing radiosensitivity. Growth arrest mediated by p21 strongly suggests that cells within or recruited into the cell cycle during treatment are at high risk of loss and do not contribute significantly to repopulation. It is possible that quiescent (G0) cells at treatment completion accounted for the accelerated post-treatment repopulation. Recent knowledge of epidermal tissue regeneration and cell cycle progression during genotoxic and mitogen stress allows for a credible explanation of the current finding. Melanocytes were radioresistant regarding cell depletion. © 2020 by Radiation Research Society. All rights of reproduction in any form reserved.
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| 38. |
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