| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
- Arvidson, Magnus, et al.
(author)
-
The VINNOVA water mist research project : A description of the 500 m3 machinery space tests
- 2003
-
Reports (peer-reviewed)abstract
- This report describes water mist and water spray system tests inside a simulated machinery space. The tests were conducted inside a compartment measuring 8,0 m by 12,5 m with a ceiling height of 5,0 m. The corresponding volume was 500 m3. The walls and the ceiling were constructed from steel plates. The test compartment replicates an intermediate size machinery space compartment onboard a ship. Either diesel oil or heptane pool fires were used as the fire source. The fires had nominal heat release rates of 500 kW, 1 MW and 2 MW, respectively and were either fully exposed to the water spray or completely shielded by a horizontal obstruction steel plate measuring 2 m by 2 m. Three different systems were tested; (1) a water spray system flowing 500 L/min at 2 bar (this system was designed according to the SOLAS convention), (2) a low-pressure system flowing 97 L/min at 12 bar and (3) a high-pressure system flowing 60 L/min at 70 bar. In addition to the tests using these three systems, free burn tests were conducted inside the compartment. This report contains a description of the test set-up, its instrumentation, the fire test procedures and a limited presentation of the results. A more thorough analysis of the test results will be made in subsequent reports, articles and papers.
|
|
| 11. |
|
|
| 12. |
|
|
| 13. |
- Aspeborg, Henrik, 1970-, et al.
(author)
-
Vegetabile material, plants and a method of producing a plant having altered lignin properties
- 2008
-
Patent (pop. science, debate, etc.)abstract
- The present invention is related to a set of genes, which when modified in plants gives altered lignin properties. The invention provides DNA construct such as a vector useful in the method of the invention. Further, the invention relates to a plant cell or plant progeny of the plants and wood produced by the plants according to the invention Lower lignin levels will result in improved saccharification for bio-refining and ethanol production and improved pulp and paper. Increased lignin levels will utilise lignin properties for energy production. The genes and DNA constructs may be used for the identification of plants having altered lignin characteristics as compared to the wild-type. According to the invention genes and DNA constructs may also be used as candidate genes in marker assisted breeding.
|
|
| 14. |
- Curci, Pasquale Luca, et al.
(author)
-
Identification of growth regulators using cross-species network analysis in plants
- 2022
-
In: Plant Physiology. - : Oxford University Press. - 0032-0889 .- 1532-2548. ; 190:4, s. 2350-2365
-
Journal article (peer-reviewed)abstract
- With the need to increase plant productivity, one of the challenges plant scientists are facing is to identify genes that play a role in beneficial plant traits. Moreover, even when such genes are found, it is generally not trivial to transfer this knowledge about gene function across species to identify functional orthologs. Here, we focused on the leaf to study plant growth. First, we built leaf growth transcriptional networks in Arabidopsis (Arabidopsis thaliana), maize (Zea mays), and aspen (Populus tremula). Next, known growth regulators, here defined as genes that when mutated or ectopically expressed alter plant growth, together with cross-species conserved networks, were used as guides to predict novel Arabidopsis growth regulators. Using an in-depth literature screening, 34 out of 100 top predicted growth regulators were confirmed to affect leaf phenotype when mutated or overexpressed and thus represent novel potential growth regulators. Globally, these growth regulators were involved in cell cycle, plant defense responses, gibberellin, auxin, and brassinosteroid signaling. Phenotypic characterization of loss-of-function lines confirmed two predicted growth regulators to be involved in leaf growth (NPF6.4 and LATE MERISTEM IDENTITY2). In conclusion, the presented network approach offers an integrative cross-species strategy to identify genes involved in plant growth and development.
|
|
| 15. |
- Dahlstedt, Magnus, 1975-, et al.
(author)
-
Democracy the Swedish way? : The exclusion of 'immigrants' in Swedish politics
- 2007
-
In: Scandinavian Political Studies. - : Wiley. - 0080-6757 .- 1467-9477. ; 30:2, s. 175-203
-
Research review (peer-reviewed)abstract
- Official declarations state that Sweden is today a multicultural society. At the same time, ethnic hierarchies have become increasingly conspicuous in contemporary Sweden. Recently, a governmental inquiry on structural discrimination in Swedish society presented a report analysing the relationship between the multi-ethnic composition of the Swedish population and participation in Swedish politics. This article discusses some of the main findings of the report. On the basis of a number of case studies, it illustrates how inequalities in terms of participation and influence in Swedish politics are (re)produced. One of the main conclusions drawn in the article is that all citizens that participate in Swedish politics are faced with a series of routines, conventions and idea(l)s categorising citizens according to their perceived closeness to a Swedish `normality'. Thus, democracy not only constitutes a formalised system of impartial procedures and conventions, routines and norms that regulate the political process in a way that guarantees freedom and equality to all participants. Rather, political participation also reflects exclusionary practices long well-documented in, for example, the housing and labour markets. In order to understand these practices, it is necessary to examine the historical interconnections between nationalism and democracy. By means of the recurrent characterisation of Swedish democracy as specifically Swedish, it becomes the job of Swedes to `enlighten' the `immigrants' to become `Swedish democrats'. This specific conceptualisation of democracy is founded on the ideal of an archaic national community, which in contemporary multi-ethnic Sweden is not capable of including the whole population on equal terms.
|
|
| 16. |
- Dahlstedt, Magnus, 1975-, et al.
(author)
-
Demokrati på svenska? Avslutande reflektioner
- 2005
-
In: Demokrati på svenska? Om strukturell diskriminering och politiskt deltagande. - Stockholm : Fritzes. - 9138224887 ; , s. 261-300
-
Book chapter (other academic/artistic)abstract
- Utredningen om Makt, integration och strukturell diskriminering startade i april 2004 och har i uppdrag att identifiera och kartlägga mekanismer bakom strukturell/institutionell diskriminering på grund av etnisk och religiös tillhörighet. Rapporten Demokrati på svenska? behandlar den svenska demokratins grundläggande dilemma, nämligen deklarationen av en formell politisk jämlikhet och en reell ojämlikhet som drabbar många människor med invandrarbakgrund och exkluderar dem från politisk deltagande.
|
|
| 17. |
- Dahlstedt, Magnus, 1975-, et al.
(author)
-
Den entreprenörskapande skolan : Styrning, subjektskapande och entreprenörskapspedagogik
- 2011
-
In: Pedagogisk forskning i Sverige. - 1401-6788 .- 2001-3345. ; 16:3, s. 179-198
-
Journal article (peer-reviewed)abstract
- Departing from Michel Foucault’s concept of governmentality, the focus of this article is the introduction of entrepreneurial education in Swedish education policy at the turn of the millennium. We analyze the various meanings attached to the concepts of entrepreneur and entrepreneurship in education policy documents, as well as the main arguments for introducing entrepreneurial education. In policy documents, the entrepreneur is portrayed as being flexible, creative, enterprising and independent, as having the ability to take initiative, solve problems and make decisions. Here, there is an emphasis made on economical utility, and its priority over other values. In conclusion, entrepreneurial education may be seen as a particular kind of governmentality, connecting students and their subjectivity to the rationality of the market.
|
|
| 18. |
- Dahlstedt, Magnus, 1975-, et al.
(author)
-
Det är inte mitt samhälle : Misstro och levd erfarenhet i mångetniska förortsmiljöer
- 2005
-
In: Demokrati på svenska? Om strukturell diskriminering och politiskt deltagande. - Stockholm : Fritzes. - 9138224887 ; , s. 113-152
-
Book chapter (other academic/artistic)abstract
- Utredningen om Makt, integration och strukturell diskriminering startade i april 2004 och har i uppdrag att identifiera och kartlägga mekanismer bakom strukturell/institutionell diskriminering på grund av etnisk och religiös tillhörighet. Rapporten Demokrati på svenska? behandlar den svenska demokratins grundläggande dilemma, nämligen deklarationen av en formell politisk jämlikhet och en reell ojämlikhet som drabbar många människor med invandrarbakgrund och exkluderar dem från politisk deltagande.
|
|
| 19. |
- Dahlstedt, Magnus, 1975-, et al.
(author)
-
Governmentality og entreprenörskap
- 2012
-
In: Inspirert av Foucault. - Oslo : Fagbokforlaget. - 9788245011883 ; , s. -258
-
Book chapter (other academic/artistic)abstract
- I denne boken presenterer artikkelforfatterne nyere pedagogisk empiri med utgangspunkt i Foucaults ideer. Forfatterne analyserer ulike utsnitt av hverdagen i barnehage, grunnskole, videregående skole og høyere utdanning. Boken er inndelt i metodologiske, læreplanteoretiske, barnehagepedagogiske og spesialpedagogiske diskurser. Ved å bruke Foucaults ideer på det empiriske materialet synliggjør forfatterne ulike måter å vurdere problemstilling, teori, metode, empiri og analyse på. Både masterstudenter, doktorgradsstudenter, undervisere i høyere utdanning og forskere kan ha nytte av boken.
|
|
| 20. |
- Dahlstedt, Magnus, 1975-, et al.
(author)
-
Schooling entrepreneurs : Entrepreneurship, governmentality and education policy in Sweden at the turn of the millennium.
- 2012
-
In: Journal of Pedagogy. - : Walter de Gruyter GmbH. - 1338-1563 .- 1338-2144. ; 3:2, s. 242-262
-
Journal article (peer-reviewed)abstract
- Departing from Michel Foucault’s concept of governmentality, the focus of this article is the introduction of entrepreneurial education in Swedish education policy at the turn of the millennium. We analyze the various meanings attached to the concepts of “entrepreneur” and “entrepreneurship” in education policy documents, as well as the main arguments for introducing entrepreneurial education. In policy documents, the “entrepreneur” is portrayed as being flexible, creative, enterprising and independent, as having the ability to take initiative, solve problems and make decisions. Here, there is an emphasis made on economical utility, and its priority over other values. With an increasing mobilization of entrepreneurship in school, previous pedagogical and educational doctrines - focusing on equality, universalism and redistribution - are challenged. Other visions, stating other educational purposes and goals emerge. In the vision of the entrepreneurial school, it becomes logical and natural to emphasize the value education has for the economic system. In conclusion, entrepreneurial education may be seen as a particular kind of governmentality, connecting students and their subjectivity to the rationality of the market - fostering subjects in line with the imperatives of the “advances liberal society”.
|
|
| 21. |
- Dahlstedt, Magnus, 1975-, et al.
(author)
-
Skola i samverkan : Miljonprogrammet och visionen om den öppna skolan
- 2011
-
Book (other academic/artistic)abstract
- Under den senare av delen av 1990-talet och början på 2000 talet var "skolan mitt i förorten" något av ett slagord inom utbildningspolitiken. Tanken var att skolorna i så kallade "utsatta områden" skulle utvecklas till en resurs och ett kraftcentrum i lokalsamhället. Skolorna skulle sjuda av aktivitet efter lektionstidens slut. Föreningslivet, lokala företag och engagerade medborgare skulle bli delaktiga i skolans verksamhet, och med sina särskilda kunskaper bidra till skolans verksamhet. Förhoppningen var att denna samverkan skulle stärka såväl lokalsamhället som skolan, och möjligheterna till inkludering i samhället skulle bli större för ungdomar med utländsk bakgrund. Boken tar sin utgångspunkt i denna utbildningspolitiska inriktning. Magnus Dahlstedt och Fredrik Hertzberg analyserar och diskuterar politikens målsättningar, utformning och premisser, med ett särskilt fokus på frågor om svenskhet, invandrarskap, tillhörighet och rättvisa. Vad ville man åstadkomma med de ambitioner som sammanfattades i slagorden samverkan och "skolan mitt i förorten"? Vilket samhälle ville man skapa? Hur såg man på olika elevgruppers möjligheter att bli fullvärdiga medborgare i detta samhälle? Vad passade in, och vad skulle förändras? På vilka sätt fick lokalsamhällets aktörer vara med i skolans verksamhet? Vilka möjligheter hade de att påverka? Med sina försök att svara på dessa frågor har Dahlstedt och Hertzberg skrivit en bok som kan ge både nya perspektiv och konkreta råd till dem som är intresserade av skolfrågor, inte minst av frågan om hur skolan ska kunna ge alla elever likvärdiga möjligheter inför sitt framtida liv och arbetsliv.
|
|
| 22. |
- Dahlstedt, Magnus, 1975-, et al.
(author)
-
Strukturell diskriminering och politiskt deltagande : Inledande tankegångar
- 2005
-
In: Demokrati på svenska? Om strukturell diskriminering och politiskt deltagande. - Stockholm : Fritzes. - 9138224887 ; , s. -301
-
Book chapter (other academic/artistic)abstract
- Utredningen om Makt, integration och strukturell diskriminering startade i april 2004 och har i uppdrag att identifiera och kartlägga mekanismer bakom strukturell/institutionell diskriminering på grund av etnisk och religiös tillhörighet. Rapporten Demokrati på svenska? behandlar den svenska demokratins grundläggande dilemma, nämligen deklarationen av en formell politisk jämlikhet och en reell ojämlikhet som drabbar många människor med invandrarbakgrund och exkluderar dem från politisk deltagande.
|
|
| 23. |
|
|
| 24. |
|
|
| 25. |
|
|
| 26. |
- Derba-Maceluch, Marta, et al.
(author)
-
Impact of xylan on field productivity and wood saccharification properties in aspen
- 2023
-
In: Frontiers in Plant Science. - : Frontiers Media SA. - 1664-462X. ; 14
-
Journal article (peer-reviewed)abstract
- Xylan that comprises roughly 25% of hardwood biomass is undesirable in biorefinery applications involving saccharification and fermentation. Efforts to reduce xylan levels have therefore been made in many species, usually resulting in improved saccharification. However, such modified plants have not yet been tested under field conditions. Here we evaluate the field performance of transgenic hybrid aspen lines with reduced xylan levels and assess their usefulness as short-rotation feedstocks for biorefineries. Three types of transgenic lines were tested in four-year field tests with RNAi constructs targeting either Populus GT43 clades B and C (GT43BC) corresponding to Arabidopsis clades IRX9 and IRX14, respectively, involved in xylan backbone biosynthesis, GATL1.1 corresponding to AtGALT1 involved in xylan reducing end sequence biosynthesis, or ASPR1 encoding an atypical aspartate protease. Their productivity, wood quality traits, and saccharification efficiency were analyzed. The only lines differing significantly from the wild type with respect to growth and biotic stress resistance were the ASPR1 lines, whose stems were roughly 10% shorter and narrower and leaves showed increased arthropod damage. GT43BC lines exhibited no growth advantage in the field despite their superior growth in greenhouse experiments. Wood from the ASPR1 and GT43BC lines had slightly reduced density due to thinner cell walls and, in the case of ASPR1, larger cell diameters. The xylan was less extractable by alkali but more hydrolysable by acid, had increased glucuronosylation, and its content was reduced in all three types of transgenic lines. The hemicellulose size distribution in the GALT1.1 and ASPR1 lines was skewed towards higher molecular mass compared to the wild type. These results provide experimental evidence that GATL1.1 functions in xylan biosynthesis and suggest that ASPR1 may regulate this process. In saccharification without pretreatment, lines of all three constructs provided 8-11% higher average glucose yields than wild-type plants. In saccharification with acid pretreatment, the GT43BC construct provided a 10% yield increase on average. The best transgenic lines of each construct are thus predicted to modestly outperform the wild type in terms of glucose yields per hectare. The field evaluation of transgenic xylan-reduced aspen represents an important step towards more productive feedstocks for biorefineries. Copyright © 2023 Derba-Maceluch, Sivan, Donev, Gandla, Yassin, Vaasan, Heinonen, Andersson, Amini, Scheepers, Johansson, Vilaplana, Albrectsen, Hertzberg, Jönsson and Mellerowicz.
|
|
| 27. |
- Donev, Evgeniy N., et al.
(author)
-
Field testing of transgenic aspen from large greenhouse screening identifies unexpected winners
- 2023
-
In: Plant Biotechnology Journal. - : Wiley. - 1467-7644 .- 1467-7652. ; 21:5, s. 1005-1021
-
Journal article (peer-reviewed)abstract
- Trees constitute promising renewable feedstocks for biorefinery using biochemical conversion, but their recalcitrance restricts their attractiveness for the industry. To obtain trees with reduced recalcitrance, large-scale genetic engineering experiments were performed in hybrid aspen blindly targeting genes expressed during wood formation and 32 lines representing seven constructs were selected for characterization in the field. Here we report phenotypes of five-year old trees considering 49 traits related to growth and wood properties. The best performing construct considering growth and glucose yield in saccharification with acid pretreatment had suppressed expression of the gene encoding an uncharacterized 2-oxoglutarate-dependent dioxygenase (2OGD). It showed minor changes in wood chemistry but increased nanoporosity and glucose conversion. Suppressed levels of SUCROSE SYNTHASE, (SuSy), CINNAMATE 4-HYDROXYLASE (C4H) and increased levels of GTPase activating protein for ADP-ribosylation factor ZAC led to significant growth reductions and anatomical abnormalities. However, C4H and SuSy constructs greatly improved glucose yields in saccharification without and with pretreatment, respectively. Traits associated with high glucose yields were different for saccharification with and without pretreatment. While carbohydrates, phenolics and tension wood contents positively impacted the yields without pretreatment and growth, lignin content and S/G ratio were negative factors, the yields with pretreatment positively correlated with S lignin and negatively with carbohydrate contents. The genotypes with high glucose yields had increased nanoporosity and mGlcA/Xyl ratio, and some had shorter polymers extractable with subcritical water compared to wild-type. The pilot-scale industrial-like pretreatment of best-performing 2OGD construct confirmed its superior sugar yields, supporting our strategy.
|
|
| 28. |
- Evegren, Franz, et al.
(author)
-
Fire testing of external combustible ship surfaces
- 2014
-
In: Proceedings of 11th International Symposium on Fire Safety Science. Christchurch, New Zealand. 2014-02-13. - : International Association for Fire Safety Science. ; , s. 905-918
-
Conference paper (peer-reviewed)abstract
- Reduced weight makes use of fiber reinforced polymer composite desirable in maritime construction applications. Exterior ship surfaces in combustible materials are although not covered by firesafety regulations and their fire protection is therefore a key issue. This paper reports how SP FIRE 105, a standardized test method for testing reaction to fire properties of façade systems, was adjusted and used to evaluate the potential for fire growth on external combustible ship surfaces; in particular fiber reinforced polymer composite surfaces protected with active or passive measures. The trials show that the test method is highly suitable but that some adjustments could be made to reduce uncertainties; in particular to use a gas burner instead of a heptane pool fire source and to add a strong criterion for when to activate active measures. Further efforts should also be made to develop suitable performance criteria, which were suggested to be based on the produced heat and the gas temperatures at the top of the panel.
|
|
| 29. |
- Hertzberg, Magnus, et al.
(author)
-
cDNA microarray analysis of small tissue samples using a cDNA tag target amplification protocol
- 2001
-
In: The Plant Journal. - : Wiley. - 0960-7412 .- 1365-313X. ; 25:5, s. 585-591
-
Journal article (peer-reviewed)abstract
- Microarray technology is becoming an important comprehensive tool to study gene expression in plants. However, the use of this technology is limited by the large amount of sample tissue needed for microarray analysis. Generally, 50-200 mug of total RNA and 1-2 mug of mRNA is required for each hybridisation, which is equivalent to 50-100 mg of plant tissue. This requirement for large amounts of starting material severely constrains the use of microarrays for transcript profiling in specific tissues and cell types during plant development. Here we report on a robust and reliable target amplification method that enables transcript profiling from sub-mg amounts of plant tissue. Using 0.1 mug of total RNA we show that twofold expression differences are possible to distinguish with 99% confidence. We also demonstrate the application of this method in an analysis of secondary phloem development in hybrid aspen using defined tissue sections, corresponding to 2-4 cell layers with a fresh weight of similar to 0.5 mg.
|
|
| 30. |
- Hertzberg, Tommy, et al.
(author)
-
Brandsäkerhet till sjöss
- 2005
-
In: BrandPosten. ; :33, s. 4-5
-
Journal article (pop. science, debate, etc.)
|
|
| 31. |
- Hertzberg, Tommy, et al.
(author)
-
Fire safety at sea
- 2006
-
In: BrandPosten. ; :33, s. 4-5
-
Journal article (pop. science, debate, etc.)
|
|
| 32. |
- Hertzberg, Tommy, et al.
(author)
-
Fire tests for ro‑pax composites
- 2008
-
In: The Naval Architect. ; :4, s. 32-35:38, s. 22-24
-
Journal article (other academic/artistic)
|
|
| 33. |
|
|
| 34. |
|
|
| 35. |
|
|
| 36. |
|
|
| 37. |
- Jonasson, Simon, et al.
(author)
-
The Effect of High Lignin Content on Oxidative Nanofibrillation of Wood Cell Wall
- 2021
-
In: Nanomaterials. - : MDPI. - 2079-4991. ; 11:5
-
Journal article (peer-reviewed)abstract
- Wood from field-grown poplars with different genotypes and varying lignin content (17.4 wt % to 30.0 wt %) were subjected to one-pot 2,2,6,6-Tetramethylpiperidin-1-yl)oxyl catalyzed oxidation and high-pressure homogenization in order to investigate nanofibrillation following simultaneous delignification and cellulose oxidation. When comparing low and high lignin wood it was found that the high lignin wood was more easily fibrillated as indicated by a higher nanofibril yield (68% and 45%) and suspension viscosity (27 and 15 mPa·s). The nanofibrils were monodisperse with diameter ranging between 1.2 and 2.0 nm as measured using atomic force microscopy. Slightly less cellulose oxidation (0.44 and 0.68 mmol·g−1) together with a reduced process yield (36% and 44%) was also found which showed that the removal of a larger amount of lignin increased the efficiency of the homogenization step despite slightly reduced oxidation of the nanofibril surfaces. The surface area of oxidized high lignin wood was also higher than low lignin wood (114 m2·g−1 and 76 m2·g−1) which implicates porosity as a factor that can influence cellulose nanofibril isolation from wood in a beneficial manner.
|
|
| 38. |
- Niculaes, Claudiu, et al.
(author)
-
Phenylcoumaran Benzylic Ether Reductase Prevents Accumulation of Compounds Formed under Oxidative Conditions in Poplar Xylem
- 2014
-
In: The Plant Cell. - : Oxford University Press (OUP). - 1040-4651 .- 1532-298X. ; 26:9, s. 3775-3791
-
Journal article (peer-reviewed)abstract
- Phenylcoumaran benzylic ether reductase (PCBER) is one of the most abundant proteins in poplar (Populus spp) xylem, but its biological role has remained obscure. In this work, metabolite profiling of transgenic poplar trees downregulated in PCBER revealed both the in vivo substrate and product of PCBER. Based on mass spectrometry and NMR data, the substrate was identified as a hexosylated 8-5-coupling product between sinapyl alcohol and guaiacylglycerol, and the product was identified as its benzyl-reduced form. This activity was confirmed in vitro using a purified recombinant PCBER expressed in Escherichia coli. Assays performed on 20 synthetic substrate analogs revealed the enzyme specificity. In addition, the xylem of PCBER-downregulated trees accumulated over 2000-fold higher levels of cysteine adducts of monolignol dimers. These compounds could be generated in vitro by simple oxidative coupling assays involving monolignols and cysteine. Altogether, our data suggest that the function of PCBER is to reduce phenylpropanoid dimers in planta to form antioxidants that protect the plant against oxidative damage. In addition to describing the catalytic activity of one of the most abundant enzymes in wood, we provide experimental evidence for the antioxidant role of a phenylpropanoid coupling product in planta.
|
|
| 39. |
- Robinson, Kathryn M., 1975-, et al.
(author)
-
Variation in non-target traits in genetically modified hybrid aspens does not exceed natural variation
- 2021
-
In: New Biotechnology. - : Elsevier. - 1871-6784 .- 1876-4347. ; 64, s. 27-36
-
Journal article (peer-reviewed)abstract
- Genetically modified hybrid aspens (Populus tremula L. x P. tremuloides Michx.), selected for increased growth under controlled conditions, have been grown in highly replicated field trials to evaluate how the target trait (growth) translated to natural conditions. Moreover, the variation was compared among genotypes of ecologically important non-target traits: number of shoots, bud set, pathogen infection, amount of insect herbivory, composition of the insect herbivore community and flower bud induction. This variation was compared with the variation in a population of randomly selected natural accessions of P. tremula grown in common garden trials, to estimate how the “unintended variation” present in transgenic trees, which in the future may be commercialized, compares with natural variation. The natural variation in the traits was found to be typically significantly greater. The data suggest that when authorities evaluate the potential risks associated with a field experiment or commercial introduction of transgenic trees, risk evaluation should focus on target traits and that unintentional variation in non-target traits is of less concern.
|
|
| 40. |
- Rui Climaco, Pinto, et al.
(author)
-
Design of experiments on 135 cloned poplar trees to map environmental influence in greenhouse
- 2011
-
In: Analytica Chimica Acta. - : Elsevier. - 0003-2670 .- 1873-4324. ; 685:2, s. 127-131
-
Journal article (peer-reviewed)abstract
- To find and ascertain phenotypic differences, minimal variation between biological replicates is always desired. Variation between the replicates can originate from genetic transformation but also from environmental effects in the greenhouse. Design of experiments (DoE) has been used in field trials for many years and proven its value but is underused within functional genomics including greenhouse experiments. We propose a strategy to estimate the effect of environmental factors with the ultimate goal of minimizing variation between biological replicates, based on DoE. DoE can be analyzed in many ways. We present a graphical solution together with solutions based on classical statistics as well as the newly developed OPLS methodology.In this study, we used DoE to evaluate the influence of plant specific factors (plant size, shoot type, plant quality, amount of fertilizer) and rotation of plant positions on height and section area of 135 cloned wild type poplar trees grown in the greenhouse. Statistical analysis revealed that plant position was the main contributor to variability among biological replicates and applying a plant rotation scheme could reduce this variation.
|
|
| 41. |
- Sania Ali, Sofia, et al.
(author)
-
Arbete och övergångar – viljan att bidra
- 2018
-
In: Förortsdrömmar. - Linköping : Linköping University Electronic Press. - 9789176853542 ; , s. 165-179
-
Book chapter (other academic/artistic)abstract
- I detta kapitel är det återigen ungas berättelser om samtiden och om framtiden som står i förgrunden, denna gång med fokus på ungas berättelser om arbete och mer specifikt om arbete som en väg in i samhället. Utgångspunkten är, liksom i föregående kapitel, ungas egna erfarenheter och meningsskapande. Vi återvänder här till Staden, precis som i kapitel 5, för att ta del av berättelser om utmaningar i inträdet i arbetslivet bland en särskild kategori av unga som på senare tid har uppmärksammats som en växande riskgrupp, närmare bestämt unga med utländsk bakgrund som varken studerar eller arbetar (Bäckman 2010; Nilsson & Bäckman 2014; Schröder 2014). I fokus för kapitlet står de ungas berättelser om sociala relationer – i form av relationer till arbetslivet och till samhället i stort, till sina närstående (föräldrar, släkt och vänner) men även mer avlägsna personer i omgivningen – och deras betydelse för de ungas framtida inträde i arbetslivet.
|
|
| 42. |
- Srivastava, Vaibhav, et al.
(author)
-
Downregulation of high-isoelectric-point extracellular superoxide dismutase mediates alterations in the metabolism of reactive oxygen species and developmental disturbances in hybrid aspen
- 2007
-
In: The Plant Journal. - : Blackwell Publishing. - 0960-7412 .- 1365-313X. ; 49:1, s. 135-148
-
Journal article (peer-reviewed)abstract
- Transgenic hybrid aspen (Populus tremula L. x P. tremuloides Michx.) plants expressing a high-isoelectric-point superoxide dismutase (hipI-SOD) gene in antisense orientation were generated to investigate its function. Immunolocalization studies showed the enzyme to be localized extracellularly, in the secondary cell wall of xylem vessels and phloem fibers. The antisense lines of hipI-SOD exhibited a distinct phenotype; growth rate was reduced, stems were thinner and leaves smaller than in wild-type (WT) plants. The abundance of hipI-SOD was reduced in the bark and xylem of plants from these antisense lines. The vascular tissue of transgenic lines became lignified earlier than in WT plants and also showed an increased accumulation of reactive oxygen species (ROS). Xylem fibers and vessels were shorter and thinner in the transgenic lines than in WT plants. The total phenolic content was enhanced in the antisense lines. Furthermore, microarray analysis indicated that several enzymes involved in cell signaling, lignin biosynthesis and stress responses were upregulated in apical vascular tissues of transgenic plants. The upregulation of selected genes involved in lignin biosynthesis was also verified by real-time PCR. The results suggest that, in the transgenic plants, a premature transition into maturation occurs and the process is discussed in terms of the effects of increased accumulation of ROS due to reduced expression of hipI-SOD during development and differentiation.
|
|
| 43. |
|
|
| 44. |
- Trygg, Johan, et al.
(author)
-
Vegetabile material, plants and a method of producing a plant having altered lignin properties
- 2012
-
Patent (pop. science, debate, etc.)abstract
- The present invention is related to a set of genes, which when modified in plants gives altered lignin properties. The invention provides DNA construct such as a vector useful in the method of the invention. Further, the invention relates to a plant cell or plant progeny of the plants and wood produced by the plants according to the invention Lower lignin levels will result in improved saccharification for bio-refining and ethanol production and improved pulp and paper. Increased lignin levels will utilise lignin properties for energy production. The genes and DNA constructs may be used for the identification of plants having altered lignin characteristics as compared to the wild-type. According to the invention genes and DNA constructs may also be used as candidate genes in marker assisted breeding.
|
|
| 45. |
-
Utbildning, arbete, medborgarskap : strategier för social inkludering i den mångetniska staden
- 2016. - 3
-
Editorial collection (other academic/artistic)abstract
- Tillgång till utbildning är en förutsättning för jämlika levnadsvillkor och ett fullvärdigt medborgarskap. Men med en allt mer polari¬serad och etniskt skiktad arbetsmarknad är utbildning inte längre en tillräcklig förutsättning för social mobilitet, trots att samhället är mer beroende av kunskap och utbildning än någonsin.I denna bok beskrivs relationen mellan utbildning och möjligheten att uppnå de levnadsvillkor som det sociala medborgarskapet föreskriver. Vilka strategier utvecklar barn till invandrare för att övervinna hinder i samhället och erfarenheter av exkludering och stigmatisering? Framför allt fokuseras övergången mellan skola och arbete: Hur präglas utbildningsval och syn på arbete och karriär av sociala bakgrundsfaktorer som klass, kön och etnisk tillhörighet? Vilken betydelse har boendemiljön och den lokala skolan för valet av skola? Hur inverkar föräldragrupper, föreningar och andra sociala rörelser på övergången mellan skola och arbetsmarknad?
|
|
| 46. |
-
Utbildning, arbete, medborgarskap : strategier för social inkludering i den mångetniska staden
- 2007. - 1
-
Editorial collection (other academic/artistic)abstract
- Tillgång till utbildning är en förutsättning för jämlika levnadsvillkor och ett fullvärdigt medborgarskap. Men med en allt mer polari serad och etniskt skiktad arbetsmarknad är utbildning inte längre en tillräcklig förutsättning för social mobilitet, trots att samhället är mer beroende av kunskap och utbildning än någonsin.I denna bok beskrivs relationen mellan utbildning och möjligheten att uppnå de levnadsvillkor som det sociala medborgarskapet föreskriver. Vilka strategier utvecklar barn till invandrare för att övervinna hinder i samhället och erfarenheter av exkludering och stigmatisering? Framför allt fokuseras övergången mellan skola och arbete: Hur präglas utbildningsval och syn på arbete och karriär av sociala bakgrundsfaktorer som klass, kön och etnisk tillhörighet? Vilken betydelse har boendemiljön och den lokala skolan för valet av skola? Hur inverkar föräldragrupper, föreningar och andra sociala rörelser på övergången mellan skola och arbetsmarknad?I denna bok beskrivs relationen mellan utbildning och möjligheten att uppnå de levnadsvillkor som det sociala medborgarskapet föreskriver. Vilka strategier utvecklar barn till invandrare för att övervinna hinder i samhället och erfarenheter av exkludering och stigmatisering? Framför allt fokuseras övergången mellan skola och arbete:Hur präglas utbildningsval och syn på arbete och karriär av sociala bakgrundsfaktorer som klass, kön och etnisk tillhörighet?Vilken betydelse har boendemiljön och den lokala skolan för valet av skola?Hur inverkar föräldragrupper, föreningar och andra sociala rörelser på övergången mellan skola och arbetsmarknad?
|
|
| 47. |
-
Utbildning, arbete, medborgarskap
- 2007. - 1
-
Editorial collection (other academic/artistic)abstract
- Tillgång till utbildning är en förutsättning för jämlika levnadsvillkor och ett fullvärdigt medborgarskap. Men med en allt mer polari serad och etniskt skiktad arbetsmarknad är utbildning inte längre en tillräcklig förutsättning för social mobilitet, trots att samhället är mer beroende av kunskap och utbildning än någonsin.I denna bok beskrivs relationen mellan utbildning och möjligheten att uppnå de levnadsvillkor som det sociala medborgarskapet föreskriver. Vilka strategier utvecklar barn till invandrare för att övervinna hinder i samhället och erfarenheter av exkludering och stigmatisering? Framför allt fokuseras övergången mellan skola och arbete: Hur präglas utbildningsval och syn på arbete och karriär av sociala bakgrundsfaktorer som klass, kön och etnisk tillhörighet? Vilken betydelse har boendemiljön och den lokala skolan för valet av skola? Hur inverkar föräldragrupper, föreningar och andra sociala rörelser på övergången mellan skola och arbetsmarknad?
|
|
| 48. |
- Wang, Jiehua, et al.
(author)
-
Reduced expression of the SHORT-ROOT gene increases the rates of growth and development in hybrid poplar and arabidopsis
- 2011
-
In: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6:12
-
Journal article (peer-reviewed)abstract
- SHORT-ROOT (SHR) is a well characterized regulator of cell division and cell fate determination in the Arabidopsis primary root. However, much less is known about the functions of SHR in the aerial parts of the plant. In this work, we cloned SHR gene from Populus trichocarpa (PtSHR1) as an AtSHR ortholog and down-regulated its expression in hybrid poplar (Populus tremula x P. tremuloides Michx-clone T89) in order to determine its physiological functions in shoot development. Sharing a 90% similarity to AtSHR at amino acid level, PtSHR1 was able to complement the Arabidopsis shr mutant. Down regulation of PtSHR1 led to a strong enhancement of primary (height) and secondary (girth) growth rates in the transgenic poplars. A similar approach in Arabidopsis showed a comparable accelerated growth and development phenotype. Our results suggest that the response to SHR could be dose-dependent and that a partial down-regulation of SHR could lead to enhanced meristem activity and a coordinated acceleration of plant growth in woody species. Therefore, SHR functions in plant growth and development as a regulator of cell division and meristem activity not only in the roots but also in the shoots. Reducing SHR expression in transgenic poplar was shown to lead to significant increases in primary and secondary growth rates. Given the current interest in bioenergy crops, SHR has a broader role as a key regulator of whole plant growth and development and SHR suppression has considerable potential for accelerating biomass accumulation in a variety of species.
|
|
