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| 2. |
- Hjerdin, A, et al.
(författare)
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Genetic Variation Among Wild and Cultivated Beets of the Section Beta as Revealed by RFLP Analysis
- 1994
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Ingår i: Journal of sugarbeet research. - 0899-1502. ; 31:1&2, s. 59-67
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Tidskriftsartikel (refereegranskat)abstract
- The level of genetic variation detected among 7 sugar beet and 4 fodder beet breeding lines was compared to the variation found among 21 accessions of wild beets of the section Beta. RFLP analysis used a set of 32 sugar beet DNA sequences as probes to score a total of 351 bands over all accessions. The band data was used to calculate genetic distances between all pairs of accessions. The distance estimates were subsequently used in a cluster analysis to produce a dendrogram of genetic distances. The analysis unambiguously defined all accessions and clearly defined a fodder beet cluster within the sugar beet cluster. The cultivated beets were all separated from the wild beets. The sugar beet breeding lines showed a considerable amount of genetic variation, comparable with the level of variation detected among the wild beet accessions.
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| 3. |
- Kraft, T., et al.
(författare)
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Estimating genetic variation in sugar beets and wild beets using pools of individuals
- 1997
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Ingår i: Genome. - 0831-2796 .- 1480-3321. ; 40:4, s. 527-533
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Tidskriftsartikel (refereegranskat)abstract
- The study describes the genetic structure in sugar beets and in wild beets (Beta vulgaris) using 30 RFLP markers. Samples consisting of pooled plant material of 100 individuals from each line and population were used to analyse 120 sugar beet breeding lines and 91 wild beet populations. Greater variation was found among the wild populations than among the breeding lines. Although the two major groups of breeding lines, monogerm and multigerm, had approximately equal amounts of genetic variation, in the monogerm group more of this variation was partitioned among the lines than within the lines. Furthermore, despite most of the variation being shared by the two groups, the two groups were found to be separated along the first two components in a principal component analysis. Computer simulations were carried out to evaluate the usefulness of the pooled-sample strategy employed in the investigation. These simulations showed the use of pooled samples to be a better alternative than that of analysing a few plants individually.
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| 4. |
- Nilsson, N. O., et al.
(författare)
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Comparing the distribution of RAPD and RFLP markers in a high density linkage map of sugar beet
- 1997
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Ingår i: Genome. - 0831-2796 .- 1480-3321. ; 40:5, s. 644-651
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Tidskriftsartikel (refereegranskat)abstract
- The distribution of RAPD markers was compared with that of RFLP markers in a high density linkage map of sugar beet. The same mapping population of 161 F2 individuals was used to generate all the marker data. The total map comprises 160 RAPD and 248 RFLP markers covering 508 cM. Both the RAPD and the RFLP markers show a high degree of clustering over the nine linkage groups. The pattern is compatible with a strong distal localization of recombination in the sugar beet. It leads generally to one major cluster of markers in the centre of each linkage group. In regions of high marker density, dominant RAPD markers present in either linkage phase and codominant RFLP markers are subclustered relative to each other. This phenomenon is shown to be attributable to: (i) effects of the mapping procedure when dominant and codominant data are combined, (ii) effects of the mapping procedure when dominant data in both linkage phases are combined, and (iii) genuine differences in the way RAPD and RFLP markers are recruited.
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