| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
- Hilson, P., et al.
(författare)
-
Versatile gene-specific sequence tags for Arabidopsis functional genomics : Trancript profiling and reverse genetics applications
- 2004
-
Ingår i: Genome Research. - : Cold Spring Harbor Laboratory. - 1088-9051 .- 1549-5469. ; 14:10B, s. 2176-2189
-
Tidskriftsartikel (refereegranskat)abstract
- Microarray transcript profiling and RNA interference are two new technologies crucial for large-scale gene function studies in multicellular eukaryotes. Both rely on sequence-specific hybridization between complementary nucleic acid strands, inciting us to create a collection of gene-specific sequence tags (GSTs) representing at least 21,500 Arabidopsis genes and which are compatible with both approaches. The GSTs were carefully selected to ensure that each of them shared no significant similarity with any other region in the Arabidopsis genome. They were synthesized by PCR amplification from genomic DNA. Spotted microarrays fabricated from the GSTs show good dynamic range, specificity, and sensitivity in transcript profiling experiments. The GSTs have also been transferred to bacterial plasmid vectors via recombinational cloning protocols. These cloned GSTs constitute the ideal starting point for a variety of functional approaches, including reverse genetics. We have subcloned GSTs on a large scale into vectors designed for gene silencing in plant cells. We show that in planta expression of GST hairpin RNA results in the expected phenotypes in silenced Arabidopsis lines. These versatile GST resources provide novel and powerful tools for functional genomics.
|
|
| 5. |
- Pinto, P. Costa, et al.
(författare)
-
Development and production of Non Evaporable Getter coatings for the vacuum chambers of the 3 GeV storage ring of MAX IV
- 2015
-
Ingår i: 6th International Particle Accelerator Conference, IPAC 2015. - 9783954501687 ; , s. 3145-3147
-
Konferensbidrag (refereegranskat)abstract
- MAX IV is presently under construction at Lund, Sweden, and the first beam for the production of synchrotron radiation is expected to circulate in 2016. The whole set of 3 GeV ring beam pipes is coated with Ti-Zr-V Non Evaporable Getter (NEG) thin film in order to fulfil the average pressure requirement of 1×10-9 mbar, despite the compact magnet layout and the large aspect ratio of the vacuum chambers. In this work, we present the optimizations of the coating process performed at CERN to coat different geometries and mechanical assembling used for the MAX IV vacuum chambers; the morphology of the thin films is analysed by Scanning Electron Microscopy; the composition and thickness is measured by Energy Dispersive X-ray analysis; the activation of the NEG thin film is monitored by X-ray Photoemission Spectroscopy; the vacuum performance of the coated beam pipes is evaluated by the measurement of hydrogen sticking coefficient. The results of the coating production characterization for the 84 units coated at CERN are presented.
|
|
