| 2. |
- Nikolaev, Nikolai, et al.
(författare)
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The sensitivity of human mesenchymal stem cells to vibration and cold storage conditions representative of cold transportation
- 2012
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Ingår i: Journal of the Royal Society Interface. - : The Royal Society. - 1742-5689 .- 1742-5662. ; 9:75, s. 2503-2515
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Tidskriftsartikel (refereegranskat)abstract
- In the current study, the mechanical and hypothermic damage induced by vibration and cold storage on human mesenchymal stem cells (hMSCs) stored at 2-8°C was quantified by measuring the total cell number and cell viability after exposure to vibration at 50 Hz (peak acceleration 140 m s-2 and peak displacement 1.4 mm), 25 Hz (peak acceleration 140 m s-2, peak displacement 5.7 mm), 10 Hz (peak acceleration 20 m s-2, peak displacement 5.1 mm) and cold storage for several durations. To quantify the viability of the cells, in addition to the trypan blue exclusion method, the combination of annexin V-FITC and propidium iodide was applied to understand the mode of cell death. Cell granularity and a panel of cell surface markers for stemness, including CD29, CD44, CD105 and CD166, were also evaluated for each condition. It was found that hMSCs were sensitive to vibration at 25 Hz, with moderate effects at 50 Hz and no effects at 10 Hz. Vibration at 25 Hz also increased CD29 and CD44 expression. The study further showed that cold storage alone caused a decrease in cell viability, especially after 48 h, and also increased CD29 and CD44 and attenuated CD105 expressions. Cell death would most likely be the consequence of membrane rupture, owing to necrosis induced by cold storage. The sensitivity of cells to different vibrations within the mechanical system is due to a combined effect of displacement and acceleration, and hMSCs with a longer cold storage duration were more susceptible to vibration damage, indicating a coupling between the effects of vibration and cold storage.
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| 3. |
- Sakai, Reika, et al.
(författare)
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Fabrication of Polylactide-Based Biodegradable Thermoset Scaffolds for Tissue Engineering Applications
- 2013
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Ingår i: Macromolecular materials and engineering. - : Wiley. - 1438-7492 .- 1439-2054. ; 298:1, s. 45-52
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Tidskriftsartikel (refereegranskat)abstract
- The fabrication of tissue engineering scaffolds based on the polymerization of crosslinked polylactide using leaching and batch foaming to generate well-controlled and interconnected biodegradable polymer scaffolds is reported. The scaffold fabrication parameters are studied in relation to the interpore connectivity, pore morphology, and structural stability of the crosslinked PLA scaffold. In vitro cell culture and in vitro degradation are used to analyze the biocompatibility and biodegradability of the scaffolds. The new crosslinked PLA thermoset scaffolds are highly suitable for bone tissue engineering applications due to their complex internal architecture, thermal stability, and biocompatibility.
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