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- Rye, Tom, et al.
(författare)
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Parking Management
- 2014
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Ingår i: Parking: Issues and Policies. - 2044-9941. ; Transport and Sustainability, Volume 5, s. 157-284
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Bokkapitel (refereegranskat)abstract
- Purpose This chapter explains how and why local parking policies are developed, the sometimes conflicting relationship between parking, revenue raising and economic development and the circumstances in which it may be appropriate to use parking policy as a demand management tool. Methodology/approach This chapter offers a review of prior research and literature on the topic parking management and further explores the impacts and difficulties of parking management. Moreover, empirical data in this chapter comes from the authors’ own survey study of southern, eastern and southeastern European cities about their parking problems and policies. Findings The findings of this chapter show that there is a need to consider what parking solutions cities may choose and what solutions might work for them. It is difficult to say that very different solutions will suit cities of different sizes, but rather that the level of implementation of the solutions must be related to the scale of the problem in each city, and its citizens’ demands. In addition, each city must work within its particular legislative context, which is why certain solutions might not work in some cities. Practical implications Parking policy and parking management are key to urban mobility and to managing its negative effects. It is possible to develop a car parking policy that will manage the negative impacts of urban car use whilst also supporting business and the economy; but this is a balancing act, which is why it is important to learn from the experience of other places, as we have shown in this chapter. Parking demand and the response of different cities to it are very important when considering the rise of car use in Europe and other parts of the world. Future solutions for parking problems are detailed at the end of this chapter. Originality/value of paper This chapter offers a comprehensive overview of prior research in parking management and connects this overview to findings of the authors own survey in south, eastern and southeastern European cities. Very few studies have made similar connections and provided in-depth insights into parking management in European cities. Moreover, the research provides useful information for planners and professionals dealing with parking issues and what solutions might work in their city.
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| 2. |
- Seth-Smith, Helena M. B., et al.
(författare)
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Whole-genome sequences of Chlamydia trachomatis directly from clinical samples without culture
- 2013
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Ingår i: Genome Research. - : Cold Spring Harbor Laboratory Press (CSHL). - 1088-9051 .- 1549-5469. ; 23:5, s. 855-866
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Tidskriftsartikel (refereegranskat)abstract
- The use of whole-genome sequencing as a tool for the study of infectious bacteria is of growing clinical interest. Chlamydia trachomatis is responsible for sexually transmitted infections and the blinding disease trachoma, which affect hundreds of millions of people worldwide. Recombination is widespread within the genome of C. trachomatis, thus whole-genome sequencing is necessary to understand the evolution, diversity, and epidemiology of this pathogen. Culture of C trachomatis has, until now, been a prerequisite to obtain DNA for whole-genome sequencing; however, as C trachomatis is an obligate intracellular pathogen, this procedure is technically demanding and time consuming. Discarded clinical samples represent a large resource for sequencing the genomes of pathogens, yet clinical swabs frequently contain very low levels of C trachomatis DNA and large amounts of contaminating microbial and human DNA. To determine whether it is possible to obtain whole-genome sequences from bacteria without the need for culture, we have devised an approach that combines immunomagnetic separation (IMS) for targeted bacterial enrichment with multiple displacement amplification (MDA) for whole-genome amplification. Using IMS-MDA ill conjunction with high-throughput multiplexed Illumina sequencing, we have produced the first whole bacterial genome sequences direct from clinical samples. We also show that this method can be used to generate genome data from nonviable archived samples. This method will prove a useful tool in answering questions relating to the biology of many difficult-to-culture or fastidious bacteria of clinical concern.
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