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- Fernaeus, Ylva, 1976-, et al.
(författare)
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Plei-Plei!
- 2012. - 1
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- Let us introduce an amazing crowd of researchers at Mobile Life Centre in Stockholm, Sweden, and some of their friends at Nokia Research Center, Microsoft Research Cambridge and Ericsson Research. These people are at the international forefront of research in the domain of mobile interactive technology – a situation that this book aims to celebrate!This is also a printed book, which means it may work a bit like a time capsule, showcasing a set of explorations that may appear peculiar and out-dated, depending on when you happen to read it. It may therefore be highlighted that all the work presented in here was conducted during the first five years of the Mobile Life Centre (2007-2012) — a time when the mobile mass market, as well as research in this field, was still new, fresh and explorative in nature.The title, Plei-Plei, refers to a playful approach towards research as characteristic in the work presented in this book. The term is also used by natives in the pacific islands of Vanuatu, to describe “mere play” in their everyday lives, as well as in their use of mobile phones. This means that the book is not just about fun and games, but rather an attempt to capture how research can be driven by a genuine curiosity of, and inspiration from, what people enjoy doing.Since many of our friends have told us that research papers are usually too long and also somewhat boring to read, we have chosen to present this work by highlighting some of our favourite results with illustrations and shorter texts that hopefully will be more inspirational and enjoyable to read. Thanks to massive help from Boris Design Studio, we are immensely impressed with the result that is now in your hand.Please Enjoy!
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| 2. |
- Ivarsson, Ylva, 1976-
(författare)
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Evolutionary Analysis and Posttranslational Chemical Modifications in Protein Redesign : A Study on Mu Class Glutathione Transferases
- 2006
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Glutathione transferases (GSTs) constitute a family of multifarious enzymes that conjugate glutathione (GSH) with a wide range of electrophiles. GSTs are grouped into different classes based on protein sequence similarities. Despite high sequence identities between GSTs of the same class they often display different substrate specificites. Human GST M1-1 is efficiently catalyzing the conjugation of GSH and various epoxide substrates, whereas the 84% sequence-identical GST M2-2 has low activities with the same substrates.Evolutionary rate analysis was used to identify hypervariable amino acid positions among GST Mu class sequences. A Thr to Ser conversion of the variable residue 210 in GST M2-2 elicited a drastic increase in catalytic activity with epoxides, which is the characteristic activity of GST M1-1. This provides support for the usefulness of evolutionary analysis in identifying functionally important residues, although the additional mutations of two other variable residues did not confer any noteworthy changes in activity.To further investigate the functional importance of residue T210 in GST M2-2 it was replaced by all other commonly occurring amino acids. The replacements caused marked changes in substrate specificity, stability, and expressivity, indicating how functionalities of a duplicated Mu class GST may easily be altered by point mutations. The stereo- and regioselectivity in epoxide-conjugation catalyzed by GSTs M1-1 and M2-2 was investigated. The results show that a serine in position 210 is beneficial for high enantioselectivity with trans-stilbene oxide. However, an alanine in position 210 is more favorable for stereo- and regioselectivity with the smaller epoxide substrate styrene-7,8-oxide. The low enantioselectivity of GST M1-1 was improved 10- and 9- fold with styrene-7,8-oxide and 1-phenylpropylene oxide, respectively, through different combination of site-specific mutations and posttranslational chemical modifications. The approach can be employed in more extensive screening experiments where a large variety of modifications easily can be tested.
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