SwePub - search: WFRF:(Jacob )

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1.	Beal, Jacob, et al. (author) Robust estimation of bacterial cell count from optical density 2020 In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1 Journal article (peer-reviewed)abstract Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
2.	Anjou, Nils Jacob, 1781-1851 (author) Dissertatio academica authentiam epistolarum Pauli ad Timotheum et Titum a dubiis Cel. Eichhornii vindicatura. Cujus partem secundam venia ampliss. facult. philos. Ups. p. p. mag. Nicol. Jacob. Anjou ... et Ericus Jacobus Alner Roslagi in audit. Gustav. die XXX Nov. MDCCCXVI. h. p. m. s. 1816 Doctoral thesis (other academic/artistic)
3.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio historico-politica de divisione imperii Hebræorum, quam, consentiente amplissimo Collegio Philosophico inclytæ Academiæ Upsaliensis, sub præsidio ... Jacobi Arrhenii ... pro gradu, ad examen publicum modeste defert ... Jacobus Walldorf, O-gothus, in audit. Gustav. maj. ad d. XX. Martii anni MDCCIX. 1709 Doctoral thesis (other academic/artistic)
4.	Arrhenius, Jacob, 1642-1725 (author) Dissertationem historicam de Sardanapalo. Cum consensu ampl. facult. phil. Upsal. sub præsidio ... Jacobi Arrhenii ... publico bonorum examini modeste subjicit, s:æ r:æ m:tis alumnus Jacobus Jacobi Arrhenius Upsaliensis, in audit. Gust. maj. a. d. 25. Maji, anni MDCCI. 1701 Doctoral thesis (other academic/artistic)
5.	Lindblom, Jacob Axelsson, 1746-1819 (author) Dissertatio politica de formis imperii mixtis earumque in rempublicam effectu, quam ... præside mag. Jacob. Axel. Lindblom ... pro gradu proponit Johannes Jacob. Schultz, Westmannus. In audit. Gust. maj. d. 18 Maji. 1782. 1782 Doctoral thesis (other academic/artistic)
6.	Neikter, Jacob Fredrik, 1744-1803 (author) Dissertatio academica De colonia svecorum in helvetiam egressa. Quam ... præside mag. Jac. Fr. Neikter ... pro gradu p. p. Jacobus Ek, stipend. Stiegler. Ostro-Gothus. In audit. Gust. majori d. VIII jun. MDCCXCVII. 1797 Doctoral thesis (other academic/artistic)
7.	Neikter, Jacob Fredrik, 1744-1803 (author) Dissertatio historica, de efficacia climatum ad variam gentium indolem, præcipue ingenia et mores. Cujus partem quartam venia amplissimæ facult. philos. Upsal. Præside mag. Jac. Fred. Neikter,... Publice ventilandam sistit Andreas Jacobus Godenius Dalekarlus. In auditorio Gust. maj. d. XVI Jun. MDCCLXXXVII. H. a. m. s., d. 4 1787 Doctoral thesis (other academic/artistic)
8.	Wallrave, Jacob, 1688-1739 (author) Dissertatio politico-juridica, de privilegiis, eorumque in republica usu et abusu, cum brevi applicatione ad statum regni Sveciæ, quam ... sub præsidio ... Dn. Jacobi Wallrave, ... publico eruditorum examini offert alumnus Helmfeldianus Jacobus Silfwerstedt, in audit. Gustav. major. ad diem 17 Decemb. an. MDCCXXXVII. horis ante meridiem solitis. 1737 Doctoral thesis (other academic/artistic)
9.	Åkerman, Jacob, 1770-1829 (author) Herniæ gangrænosæ casum ... præside Jacobo Åkerman, ... pro gradu medico p. p. auctor Jacobus Bågenholm Vermelandus. In auditor. med. die XXVII Febr. MDCCCXXVIII 1828 Doctoral thesis (other academic/artistic)
10.	Åkerman, Jacob, 1770-1829 (author) In casum morbi paralytici meditationes venia exp. facult. med. Upsal. præside Jacobo Åkerman ... pro gradu medico p.p. auctor Gustavus Jacobus Åkerman ... stip. Sparfeld. Gothlandus. In auditor. med. die XIV Mart. MDCCCXXIX. 1829 Doctoral thesis (other academic/artistic)
11.	Åkerman, Jacob, 1770-1829 (author) Observationes anatomico-pathologiæ circa uterum atque ovaria casu singulari illustratæ quas venia exp. facult. med. Upsal. præside Jacobo Åkerman ... pro gradu publicæ censuræ submittit auctor Jacobus Nicolaus Lindström Gothlandus. In audit. Gustav. die VIII Junii MDCCCXXII. Horis consuetis 1822 Doctoral thesis (other academic/artistic)
12.	Abbafati, Cristiana, et al. (author) 2020 Journal article (peer-reviewed)
13.	Alner, Eric Jacob (author) Commentariolum in vaticinia prophetæ Haggæi quod venia ampl. facult. philos. Upsal. p. p. mag. Ericus Jacobus Alner ... et Christoph. Maur. Årre stip. reg. Roslagi in audit. Gustav. die VIII Maji MDCCCXXII., p. 1 1822 Doctoral thesis (other academic/artistic)
14.	Anjou, Nils Jacob, 1781-1851 (author) Dissertatio academica authentiam epistolarum Pauli ad Timotheum et Titum a dubiis Cel. Eichhornii vindicatura. Cujus partem primam venia ampliss. facult. philos. Ups. p. p. mag. Nicol. Jacob. Anjou ... et Matthias Fredericus Ihre Gothlandus. In audit. Gustav. die XXX Nov. MDCCCXVI. h. a. m. s. 1816 Doctoral thesis (other academic/artistic)
15.	Arrhenius, Jacob, 1642-1725 (author) Abdolominum sua sorte contentum dissertatione philosophica cum venia & consensu. amplissimi collegii philosophici in regia ad Salam academia, præside ... Jacobo Arrhenio ... brevissime pro gradv exhibet s:æ r:æ m:tis alumnus Andreas Browallius Dalekarlvs. In audit: Gustav: maj: ad d, XX. Novemb: anno M DC XCVII 1697 Doctoral thesis (other academic/artistic)
16.	Arrhenius, Jacob, 1642-1725 (author) Babylon Orientalis stans & cadens dissertatione graduali delineata &, adprobante amplissimo senatu philosophico Upsaliensi, sub moderamine ... Jacobi Arrhenii ... publico subjecta examini in audit. Gustav. maj. ad diem 21 Junii an. 1698. a Samuele Petronio Calmariensi. Alumno regio. 1698 Doctoral thesis (other academic/artistic)
17.	Arrhenius, Jacob, 1642-1725 (author) Brevis dissertatio de caussis bellorum inter Persas & Græcos, quam consensu ampliss. facult. philosophicæ sub præsidio ... Jacobi Arrhenii ... pro honoribus in philosophia, publico submittit examini in auditorio Gustaviano majori a. d. 17. Nov. anno 1694. Andreas L. Törner, O. Gothus 1694 Doctoral thesis (other academic/artistic)
18.	Arrhenius, Jacob, 1642-1725 (author) Consul Romanus, quem, dissertatione historico-politica, ex consensu amplissimæ Facultatis Philosophicæ in Regia Academia Upsaliensi, sub præsidio ... Jacobi Arrhenii ... ad publicum examen modeste defert Marcus Engelsman O-Gothus: In aud. Gust. maj. ad Cal. Martias Anni MDCCV. 1705 Doctoral thesis (other academic/artistic)
19.	Arrhenius, Jacob, 1642-1725 (author) De antiqvitate monarchiæ sveo-gothicæ ... moderatore ... Dn. Jacobo Arrhenio ... publico examini exponit ... Gudmundi Choriæ alumnus Sveno Wahlberg, W. Gothus in audit. Gust. maj. ad d. XXI maji anni M DC XCVIII 1698 Doctoral thesis (other academic/artistic)
20.	Arrhenius, Jacob, 1642-1725 (author) De claritate virtutis & artis circa ardua exercitium academicum, quod cum consensu ampliss. Facult. Philos: in Regia Academia Upsalensi, præside ... Jacobo Arrhenio ... publicæ ventilationi modeste sistit ... Carolus Echman, O-Gothus, in audit. Gustav. maj. ad diem XX. Dec. anni MDCCXV. 1715 Doctoral thesis (other academic/artistic)
21.	Arrhenius, Jacob, 1642-1725 (author) De congiariis et donativis dissertatio gradualis, quam, permissu amplissimi collegii philosophici Upsaliensis, sub praesidio ... Jacobi Arrhenii ... publico bonorum examini modeste submittit Carolus Enhörning eloqu. & poës. lect. in gymn. Streng. In auditorio Gustaviano majore ad diem XIII. Novemb: anni M. DCC. 1700 Doctoral thesis (other academic/artistic)
22.	Arrhenius, Jacob, 1642-1725 (author) De excidio Carthaginis dissertatio historico-politica, quam ex consensu ampliss. Facult. Philos. in Regia Academia Upsalensi, præside ... Jacobo Arrhenio ... pro gradu, publicæ ventilatione modeste sistit ... Samuel Reuselius, O-Gothus, in audit. Gustav. maj. ad diem XII Decemb. anni MDCCXII. 1712 Doctoral thesis (other academic/artistic)
23.	Arrhenius, Jacob, 1642-1725 (author) De fine bonorum philosophica dissertatio brevissima quam ... præside ... Jacobo Arrhenio ... publico bonorum examini modeste submittit Johannes Trautzel, Sudermannus in audit. Gustav. majori ad diem [ ] anni M DC XCVIII. 1698 Doctoral thesis (other academic/artistic)
24.	Arrhenius, Jacob, 1642-1725 (author) De Hannibale Poenorum disputatio gradualis, quam consensu ampliss. Ord. Philosoph. in illustri Academia Upsaliensi, præside ... Jacobo Arrhenio ... publico bonorum examini modeste exhibet ... Magnus Leverin Ostro-Gothus. In audit. Gust. majori die 15. Maji A. 1707 horis ante meridiem solitis. 1707 Doctoral thesis (other academic/artistic)
25.	Arrhenius, Jacob, 1642-1725 (author) De jure hospitii dissertatio historica; quam, ex consensu ampliss. Senatus. Philososoph. in Regia, quæ Upsaliæ floret, Academia, præside ... Jacobo Arrhenio ... publicæ ventilationi, ad. d. 28 mensis Aprilis anni MDCCXIV. in audit Gustav majori ... modeste submittit ... Israël S. Melander, Ostro-gothus. 1714 Doctoral thesis (other academic/artistic)
26.	Arrhenius, Jacob, 1642-1725 (author) De templo Upsala, jdololatriæ quondam per septentrionem sede, dissertationem historicam, permissu amplissimi ordinis philosophici, in illustri Regiaque Academia Upsalensi, sub præsidio viri amplissimi dn. Jacobi Arrhenii ... qua par est modestia, publico bonorum submittit examini Petrus Hesselius dalekarlus. In audit. Gust. maj. ad d. 7. decemb. anno 1698. Horis ante meridiem consvetis. 1698 Doctoral thesis (other academic/artistic)
27.	Arrhenius, Jacob, 1642-1725 (author) De Vexore seu Sesostri, qvi in sacris dicitur Sesacus, dissertio historica, quam consensu amplissimæ facultatis philosophicæ in regia academia Upsaliensi sub præsidio ... Jacobi Arrhenii ... pro solitis in philosophia honoribus publico examini submittit s:æ r:æ m:tis alumnus Johannes Venelius Ost:-Gothus in auditorio Gust: maj: d: [7] Novemb. ann. 1696. 1696 Doctoral thesis (other academic/artistic)
28.	Arrhenius, Jacob, 1642-1725 (author) Disputatio historica de Belschatzare, quam cum consensu ampliss. Facultatis Philosoph. in Reg. Acad. Upsal. præside ... Jacobo Arrhenio ... pro gradu publico examini modeste submittit Petrus Schiffer Westmannus. In audit. Gustav. maj. ad 10. Junii A:o 1705. 1705 Doctoral thesis (other academic/artistic)
29.	Arrhenius, Jacob, 1642-1725 (author) Disputatio historica de imperio Salomonis, qvam bono cum Deo, consensu & permissu ampliss. facult. philosoph. in Regia academia Upsal, sub præsidio ... Jacobi Arrhenii ... pro solitis in philos. honoribus eruditorum examini modeste submittit Johannes Florander jun. In aud. Gust. maj. ad d. [11 Septembris] a. 1697, horis ante meridiem solitis. 1697 Doctoral thesis (other academic/artistic)
30.	Arrhenius, Jacob, 1642-1725 (author) Disputatio historico-politica de regni Romani destructione, qvam consensu amplissimæ facultatis philos. in regia academia Upsaliensi, sub præsidio ... Jacobi Arrhenii ... publico ... examini in audit. Gust. maj. ad diem XXV Aprilis M DC XCVI. modeste submittit Olaus Kumblæus ... . 1696 Doctoral thesis (other academic/artistic)
31.	Arrhenius, Jacob, 1642-1725 (author) Disputatio philosophica de fortuna bellica Alexandri Magni, quam ... sub moderamine ... Jacobi Arrhenius ... Daniel Sedelius ... . In auditorio Gust. majori ad d. 25. Octobr. anno 1693 1693 Doctoral thesis (other academic/artistic)
32.	Arrhenius, Jacob, 1642-1725 (author) Disputatio philosophica, de præcepti et exempli adplicatione politico necessaria, quam ex consensu & adprobatione amplissimi ordinis philosophici in acad. Ups. sub præsidio ... Jacobi Arrhenii ... pro gradu publice ventilandam proponit s.æ r.æ m.tis alumnus, Michael E. Frodlin Hels. Ad. d. [15] Novemb. 1694. in aud. Gust. maj. consvetis ante merid. horis. 1694 Doctoral thesis (other academic/artistic)
33.	Arrhenius, Jacob, 1642-1725 (author) Disputatio philosophica de Pyrrhonia secta, quam ex consensu & approbatione ampliss. Colleg. Philosoph. in illustri Reg. Acad. Upsal. præside ... Jacobo Arrhenio ... pro magisteri philosophici gradu publico bonorum examini modeste submittit ... Bothvidus Liviin, Ostrogothus. in audit. Gust. majori pridii iduum Octob. an: MDCCVIII horis ante meridiem solitis. 1708 Doctoral thesis (other academic/artistic)
34.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio academica de restituto Prester Chan recognitæ Habeshiæ, quam cum consensu ampliss: facultatis philos: in regio ad Salam Athenæo, sub præsidio ... Jacobi Arrhenii ... publico examini modeste submittit Johannes J. Jsopedius Uplandus. In audit. Gustav: maj: 14 Mart: anno MDCC. 1700 Doctoral thesis (other academic/artistic)
35.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio academica prior, de supremis Augusti. Quam ... sub præsidio ... Jacobi Arrhenii ... publico bonorum examini modeste submittit Jahannes Phoenix Er: fil: O-Gothus. In audit. Gustav: majori ad diem 22. mens: Maji An: 1691 1691 Doctoral thesis (other academic/artistic)
36.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio de erudita conversatione, quam ... sub præsidio ... Jacobi Arrhenii ... pro gradu ad publicum examen modeste defert ... Magnus M. Pontinus O: gothus. In aud. Gust. maj. d. 26. Septemb. anno MDCC 1700 Doctoral thesis (other academic/artistic)
37.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio de incrementis reip. Romanæ. Quam ex consensu ampl. fac. philos. in regia Upsal. academ. præside ... Jacobo Arrhenio ... publico examini submittit s.æ r.æ m:tis alumnus Gustavus Fult, Holm. in auditor. Gustavian. maj. d. [2] Septembr. anno M DC XCVI. 1696 Doctoral thesis (other academic/artistic)
38.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio de Mora-steen quam cum consensu ampl. collegii philosoph. in reg. acad. Upsal. sub moderamine ... Jacobi Arrhenii ... pro honoribus in philosophia consvetis, publico examini modeste subjicit s.æ r.æ m.tis alumnus Johannes L. Törner O-Gothus. In audit. Gust. maj. ad d. 13 Octob. a. 1700 1700 Doctoral thesis (other academic/artistic)
39.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio de zelo Bruti in vindicanda Romanis libertate, quam permissu ampliss. facult. philos. in regia Upsal. academia, præside ... Jacobo Arrhenio ... publico examini modeste submittit s.æ r.æ m:tis alumnus Andreas Lodin Rosl. in auditorio Gustaviano majori ad diem 18. Novemb. anni 1696. 1696 Doctoral thesis (other academic/artistic)
40.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio gradualis de Græcia triumphante; quam cum consensu amplissimæ facultatis philosophicæ in regia academia Upsal. præside ... Jacobo Arrhenio ... candidorum examini modeste offert Haquinus Brand Vermelandus; in auditorio Gustaviano majore, ad diem 20 Maji anno MDC.XCIII. 1693 Doctoral thesis (other academic/artistic)
41.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio gradualis de numero oratorio, quam, annuente ampliss. Facult. Phil. in Reg. Acad. Upsal. sub præsidio ... Jacobi Arrhenii ... ad publicum examen modeste defert ... Laurentius J. Arrhenius Upsaliensis. In audit. Gust. majori a. d. 26 Maji A: MDCCV. 1705 Doctoral thesis (other academic/artistic)
42.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio gradualis virtutes militantium breviter exhibens, quam consentiente ampliss. Facult. Philos. in Regia Academia Upsaliensi, præside ... Jacobo Arrhenio ... ad publicum examen modeste defert ... Johannes Wettrenius, O-Gothus, in aud. Gust. majori ad diem XXV Aprilis anno MDCCXIII. 1713 Doctoral thesis (other academic/artistic)
43.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio historica de Ahasvero Esther quam adprobante amplissima facultate philosophica in regia academia Upsal. sub præsidio ... Jacobi Arrhenii ... defendere conabitur Andreas Söderman Holmia-Roslagus in aud: Gustav: majori ad diem 28. Novemb: anno 1696. 1696 Doctoral thesis (other academic/artistic)
44.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio historica de captivitate Babelonica, quam annuente amplissimo ordine philosophico in regio Athenæo Upsal: sub præsidio ... Jacobi Arrhenii ... in auditorio Gustaviano majori a. d. 22. Febr. an. 1702. publico bonorum examini modeste subjicit Sven O. Norman, Sudermannus. 1702 Doctoral thesis (other academic/artistic)
45.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio historica de Dario Medo, quam consentiente amplissimo collegio philos. in regia Upsaliensi academia, sub moderamine ... Jacobi Arrhenii ... publico examini modeste submittit Johannes Norström, O-Gothus, ad d. [19] Junii 1697. In audit. Gustav. majori. 1697 Doctoral thesis (other academic/artistic)
46.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio historica de fatis populi Judaici, quam ex consensu ampliss. Facult. Philosophicæ in illustri Upsaliensi Academica sub præsidio ... Jacobi Arrhenii ... publico bonorum examini modeste subjicit ... Johannes Hagström Dalekarlus. In auditorio Gustav. maj. a. d. 8. Junii, anni 1703. 1703 Doctoral thesis (other academic/artistic)
47.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio historica de initiis monarchiae Babyloniorum, quam, cum cons. ampliss. Colleg. Philos. in Reg. Acad. Upsal. sub praesidio ... Jacobi Arrhenii ... publico examini modeste subjicit Petrus Hagberg Gestr. In audit. Gustav. maj. ad d. 25. Maji. Anni MDCCV. 1705 Doctoral thesis (other academic/artistic)
48.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio historica de initio qvartæ monarchiæ. Quam consensu ampliss: facultatis philosophicæ Upsaliensis, sub præsidio ... Jacobi Arrhenii ... publico examini modeste subjicit, s.æ r.æ m.tis alumnus Benedictus L. Trozelius, O: Gothus. In aud. Gust maj. ad diem 22. Septemb. A. MDCC. horis ante meridiem consvetis. 1700 Doctoral thesis (other academic/artistic)
49.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio historica de militia veterum Gothorum. Quam cum consensu ampliss: facult: philosophicæ in regia academia Upsaliensi sub præsidio ... Jacobi Arrhenii ... publico examini modeste submittit, Samuel Elfving, Sam. fil. Dalekarlus. In auditorio Gust. maj. ad diem 19 Nov. anni MDCXCVIII. horis ante meridiem consvetis. 1698 Doctoral thesis (other academic/artistic)
50.	Arrhenius, Jacob, 1642-1725 (author) Dissertatio historica de monarchia Assyriorum Ctesiana, quam ex consensu amplissimæ Facultatis Philosophicæ in Regia Academia Upsaliensi, sub praesidio ... Jacobi Arrhenii ... publicæ censuræ modeste subjicit Carolus Enström O-Gothus. In audit. Gustaviano majori ad d. [15] Martii Anni MDCCV. 1705 Doctoral thesis (other academic/artistic)

Beal, Jacob, et al. (author)
Robust estimation of bacterial cell count from optical density
2020
In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
Journal article (peer-reviewed)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.

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