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- Jitschin, Regina
(författare)
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Crosstalk of human mesenchymal stromal cells with the cellular components of the immune system
- 2013
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Using the potential of immune regulatory cell populations for cellular therapy constitutes an attractive tool to obliterate imbalances of immune responses in inflammatory disorders. In this context, adoptive transfer of mesenchymal stromal cells (MSCs) represents a relatively novel approach and its impact on the immune system has not been completely clarified. In this thesis we aimed to study the effects of MSCs on key immune cell types, which led us amongst others to investigate regulatory T-cells (TRegs), and myeloid cells. We show that MSCs utilize the anti-oxidative, immune regulatory enzyme hemeoxygenase-1 (HO-1) for suppressing T-cell activation directly and for inducing TRegs (=indirect T-cell suppression). An inflammatory milieu generated by alloreactive T-cells led to the so-called ‘licensing’ of the MSCs boosting their regulatory capacity. Interestingly, HO-1 expression was substantially diminished during this process and its functions were taken over by other (up-regulated) molecules such as cyclooxygenase-2 thereby highlighting (functional) MSC plasticity. Most MSC-based trials lack a systemic immune monitoring, which is key for interpreting the in vivo effects of MSCs. Performing a comprehensive flow cytometry-based immune screening in patients with acute graft-versus-host disease (aGVHD), treated with either third-party MSC or placebo infusions (in a double-blinded fashion), we were, most importantly, able to further corroborate the notion that MSCs function in vivo partly by promoting TReg-subsets. Thereby, our data underscores the need for accompanying extensive immune analyses to better comprehend such “bench-to-bedside” approaches. Accordingly, we carried out thorough, laboratory investigations when we were the first to apply MSCs in a patient with treatment-refractory hemophagocytic lymphohistocytosis. Upon MSC infusion we could observe an increase of the immune modulating cytokine interleukin (IL)-10 in the serum and a preferential appearance of regulatory type 2 macrophages in the patients’ bone marrow. Altogether, this data confirmed previous findings from in vitro and animal model studies regarding the MSCs’ impact on myeloid cell populations. Driven by these observations we sought out to assess whether MSCs induce so-called myeloid derived suppressor cells (MDSCs) in aGVHD patients. Although we did not find an MSC-associated effect, we were the first to identify monocytic CD14+HLA-DRlow/neg MDSCs accumulating after allogeneic hematopoietic transplantation. We characterized their suppressive function (via indoleamine-2,3-dioxygenase) and established a significant association with inflammatory cytokines and aGVHD. In fact, our data indicates that MDSCs are part of an immune regulating feedback mechanism that is activated during hyper-inflammations (such as in aGVHD). Overall, our results indicate that immune regulatory populations play a decisive role in various inflammatory diseases and MSCs could boost their responses. Furthermore our work suggests that combining basic and translational research is pre-requisite for understanding the MSCs’ multifaceted interactions and for optimizing their clinical use.
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| 2. |
- Simonson, Oscar E., et al.
(författare)
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In Vivo Effects of Mesenchymal Stromal Cells in Two Patients With Severe Acute Respiratory Distress Syndrome
- 2015
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Ingår i: Stem Cells Translational Medicine. - : Oxford University Press (OUP). - 2157-6564 .- 2157-6580. ; 4:10, s. 1199-1213
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Tidskriftsartikel (refereegranskat)abstract
- Mesenchymal stromal cells (MSCs) have been investigated as a treatment for various inflammatory diseases because of their immunomodulatory and reparative properties. However, many basic questions concerning their mechanisms of action after systemic infusion remain unanswered. We performed a detailed analysis of the immunomodulatory properties and proteomic profile of MSCs systemically administered to two patients with severe refractory acute respiratory distress syndrome (ARDS) on a compassionate use basis and attempted to correlate these with in vivo anti-inflammatory actions. Both patients received 2 x 10(6) cells per kilogram, and each subsequently improved with resolution of respiratory, hemodynamic, and multiorgan failure. In parallel, a decrease was seen in multiple pulmonary and systemic markers of inflammation, including epithelial apoptosis, alveolar-capillary fluid leakage, and proinflammatory cytokines, microRNAs, and chemokines. In vitro studies of the MSCs demonstrated a broad anti-inflammatory capacity, including suppression of T-cell responses and induction of regulatory phenotypes in T cells, monocytes, and neutrophils. Some of these in vitro potency assessments correlated with, and were relevant to, the observed in vivo actions. These experiences highlight both the mechanistic information that can be gained from clinical experience and the value of correlating in vitro potency assessments with clinical effects. The findings also suggest, but do not prove, a beneficial effect of lung protective strategies using adoptively transferred MSCs in ARDS. Appropriate randomized clinical trials are required to further assess any potential clinical efficacy and investigate the effects on in vivo inflammation. STEM CELLS TRANSLATIONAL MEDICINE 2015;4:1199-1213
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