SwePub
Sök i SwePub databas

  Utökad sökning

Träfflista för sökning "WFRF:(Jonsson Olle) "

Sökning: WFRF:(Jonsson Olle)

  • Resultat 1-50 av 67
Sortera/gruppera träfflistan
   
NumreringReferensOmslagsbildHitta
1.
  •  
2.
  •  
3.
  • Ahlgren, Kerstin M. (författare)
  • Immunological Studies using Human and Canine Model Disorders
  • 2011
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • The studies presented in this thesis focus on human and canine models for autoimmune disease, with the main aim to gain new knowledge about disease mechanisms and to further evaluate the dog as a model for autoimmune disease. Autoimmune Polyendocrine Syndrome type 1 (APS-1) is a hereditary human multiorgan disease caused by mutations in the autoimmune regulator (AIRE) gene. Hallmarks of APS-1 are chronic mucocutaneous candidiasis caused by Candida albicans, together with the autoimmune endocrine disorders hypoparathyroidism and adrenocortical failure. Many human diseases have an equivalent disease in dogs. Because humans share environment, and in part life style with the dogs they provide an interesting model for further genetic studies. Immune responses to Candida albicans in APS-1 patients displayed an increased secretion of the proinflammatory cytokine IL-17A and similar results were also found in AIRE deficient mice. Anticytokine autoantibodies to IL-17A, IL-17F and IL-22 were detected in APS-1 patients, and a radioligand binding assay for measuring these autoantibodies was developed and evaluated. In the canine studies we investigated whether canine diabetes mellitus could serve as a model for human autoimmune diabetes mellitus. Furthermore, we investigated type I IFN responses in Nova Scotia duck tolling retriever dogs with a systemic autoimmune disease resembling human SLE. Four assays were used in search for signs of humoral autoimmunity in diabetic dogs. However, no evidence for a type 1 diabetes-like phenotype in dogs was found. Sera from Nova Scotia duck tolling retrievers suffering from steroid-responsive meningitis arteritis elicited an increased expression of IFN-inducible genes in the canine MDCK cell line. This suggests that these dogs have an IFN signature, as seen in human SLE.
  •  
4.
  • Berglund, Lisa, et al. (författare)
  • Glucose-Dependent Insulinotropic Polypeptide (GIP) Stimulates Osteopontin Expression in the Vasculature via Endothelin-1 and CREB.
  • 2016
  • Ingår i: Diabetes. - : American Diabetes Association. - 1939-327X .- 0012-1797. ; 65:1, s. 239-254
  • Tidskriftsartikel (refereegranskat)abstract
    • Glucose-dependent insulinotropic polypeptide (GIP) is an incretin hormone with extrapancreatic effects beyond glycemic control. Here we demonstrate unexpected effects of GIP signaling in the vasculature. GIP induces the expression of the pro-atherogenic cytokine osteopontin (OPN) in mouse arteries, via local release of endothelin-1 (ET-1) and activation of cAMP response element binding protein (CREB). Infusion of GIP increases plasma OPN levels in healthy individuals. Plasma ET-1 and OPN levels are positively correlated in patients with critical limb ischemia. Fasting GIP levels are higher in individuals with a history of cardiovascular disease (myocardial infarction, stroke) when compared to controls. GIP receptor (GIPR) and OPN mRNA levels are higher in carotid endarterectomies from patients with symptoms (stroke, transient ischemic attacks, amaurosis fugax) than in asymptomatic patients; and expression associates to parameters characteristic of unstable and inflammatory plaques (increased lipid accumulation, macrophage infiltration and reduced smooth muscle cell content). While GIPR expression is predominantly endothelial in healthy arteries from human, mouse, rat and pig; remarkable up-regulation is observed in endothelial and smooth muscle cells upon culture conditions yielding a "vascular disease-like" phenotype. Moreover, a common variant rs10423928 in the GIPR gene associated with increased risk of stroke in type 2 diabetes patients.
  •  
5.
  • Bergström, Ulrica, 1970-, et al. (författare)
  • Fracture mechanisms and fracture pattern in men and women aged 50 years and older : a study of a 12-year population-based injury register, Umeå, Sweden
  • 2008
  • Ingår i: Osteoporosis International. - London : Springer International. - 0937-941X .- 1433-2965. ; 19:9, s. 1267-1273
  • Tidskriftsartikel (refereegranskat)abstract
    • Summary: In a study of a 12-year population-based injury register, Umeå, Sweden, we analyzed the fracture mechanisms and fracture pattern in men and women 50 years and older. Low-energy trauma was responsible for the major and costliest part of the fracture panorama, but the pattern differs between age groups. Introduction: Osteoporosis-related fracture is a major health problem: the number of hip fractures is expected to double to 2030. While osteoporosis is one of many risk factors, trauma is almost always involved. Therefore, we analyzed injury mechanisms in patients aged over 50. Methods: We registered injury mechanism, cause, diagnosis in all trauma patients at Umeå University hospital, Sweden. This population-based register (1993–2004) comprises a total of 113,668 injuries (29,189 fractures). Patients ≥50 years contributed to 13,279 fractures. Results: Low-energy trauma (fall <1 m) caused 53% of all fractures ≥50 years and older. In those over 75 low-energy trauma caused >80%. The seasonal variation of fractures was maximally 25%. With increasing age, proximal fractures became more common, in both upper and lower extremities. Proximal locations predominate in older age groups. Conclusions: Low-energy trauma was responsible for the largest and costliest part of the fracture panorama. In fact, almost all fractures in middle-aged and old people were caused by low-energy mechanisms; thus, most fractures in these patients have a fragility component, and the contribution of osteoporosis-related fractures is more important than previously thought. A better understanding of injury mechanisms also in low-energy trauma is a prerequisite for preventive interventions.
  •  
6.
  • Bergström, Ulrica, 1970-, et al. (författare)
  • The hip fracture incidence curve is shifting to the right : a forecast of the age-quake
  • 2009
  • Ingår i: Acta Orthopaedica. - : Medical Journals Sweden AB. - 1745-3674 .- 1745-3682. ; 80:5, s. 520-524
  • Tidskriftsartikel (refereegranskat)abstract
    • Background The number of hip fractures has doubled in the last 30–40 years in many countries. Age-adjusted incidence has been reported to be decreasing in Europe and North America, but is there a decreasing trend in all age groups? Patients and methods This population-based study included all hip-fracture patients over 50 years of age (a total of 2,919 individuals, 31% of whom were men) admitted to Umeå University Hospital, Sweden, from 1993 through 2005. Results The incidence of hip fracture declined between the periods 1993–1996 and 2001–2005: from 706 to 625 hip fractures per 105 women and from 390 to 317 hip fractures per 105 men. However, there was a 114% increase in the number of fractures in women aged 90 or older (12 and 25 hip fractures/year, respectively, in the two time periods). For the period 2001–05, women ≥ 90 years of age accounted for almost the same numbers of hip fractures as women aged 75–79 (27 fractures/year). The rate increased during this period, from 2,700 per 105 women to 3,900 per 105 women > 90 years. In men there were declining trends for both relative and absolute numbers. Interpretation Although age-adjusted incidence declined in the population > 50 years of age, absolute fracture rate and incidence increased in the very old. Women over 90 now have the same absolute number of hip fractures every year as women aged 75–79 years. There was a right-shift in hip fracture distribution towards the oldest old, probably due to an increased number of octo/nonagenarians, a new population of particularly frail old people that hardly existed earlier. Better health among septuagenarians may also have delayed the age at which fractures occurred. This changing pattern will strain orthopedic and geriatric resources even more.
  •  
7.
  • Brändström, Helena, et al. (författare)
  • Regulation of osteoprotegerin mRNA levels by prostaglandin E2 in human bone marrow stroma cells.
  • 1998
  • Ingår i: Biochemical and biophysical research communications. - : Elsevier BV. - 0006-291X. ; 247:2, s. 338-41
  • Tidskriftsartikel (refereegranskat)abstract
    • The recently cloned osteoclastogenesis inhibitory factor, or osteoprotegerin (OPG), has been shown to be a potent inhibitor of osteoclast formation. The inhibition is believed to be mediated through specific binding of OPG to a cell surface ligand on osteoblastic stromal cells. In this report we have studied the effect of the bone resorbing agent prostaglandin E2 (PGE2) on OPG mRNA levels in primary cultures of human bone marrow stroma cells (hBMSC). PGE2 dose- and time-dependently down-regulated the mRNA levels of OPG, as measured by RNAse protection assay. After 4 hours of stimulation with 1 microM PGE2, OPG mRNA levels were significantly decreased. The inhibitory effect was seen at and above 1 nM of PGE2. To elucidate whether the OPG mRNA levels are regulated via the proteinkinase A and/or the proteinkinase C pathways we stimulated cells with either forskolin (FSK) or phorbolic ester (PDbu) respectively. FSK (10 microM) decreased OPG mRNA levels to 50 % of control, whereas PE (10 nM) upregulated the mRNA levels to 250 % of control. These data show that PGE2 down-regulates the expression of OPG mRNA in hBMSC, probably via an increase in cAMP. This mechanism might be involved in PGE2-induced bone resorption.
  •  
8.
  • Brändström, Helena, et al. (författare)
  • Tumor necrosis factor-alpha and -beta upregulate the levels of osteoprotegerin mRNA in human osteosarcoma MG-63 cells.
  • 1998
  • Ingår i: Biochemical and biophysical research communications. - : Elsevier BV. - 0006-291X. ; 248:3, s. 454-7
  • Tidskriftsartikel (refereegranskat)abstract
    • Osteoprotegerin (OPG) is a recently cloned soluble member of the tumor necrosis factor receptor family. OPG has been shown to inhibit osteoclast recruitment by binding to OPG-ligand, an osteoclast differentiating factor on osteoblastic stromal cells, thereby blocking osteoclastogenesis. In this report we have examined the effect of tumor necrosis factor-alpha (TNF-alpha) and tumor necrosis factor-beta (TNF-beta) on OPG mRNA levels in the human osteosarcoma cell line MG-63. We demonstrate that both TNF-alpha and TNF-beta dose- and time-dependently upregulate the mRNA levels of OPG. The effect is significant at and above 5 pM of TNF-alpha and 1 pM of TNF-beta. The stimulatory effect on OPG mRNA levels in MG-63 cells was detected after 2 hrs of incubation with TNF-alpha or TNF-beta. These data demonstrate that the expression of OPG in osteoblasts, with subsequent effects on osteoclastogenesis, is regulated by TNFs.
  •  
9.
  •  
10.
  • Fava, Cristiano, et al. (författare)
  • Novel mutations in the SLC12A3 gene causing Gitelman's syndrome in Swedes
  • 2007
  • Ingår i: DNA Sequence. - : Informa UK Limited. - 1029-2365 .- 1042-5179. ; 18:5, s. 395-399
  • Tidskriftsartikel (refereegranskat)abstract
    • PURPOSE: Gitelman's syndrome (GS) is an inherited autosomal recessive disorder due to loss of function mutations in the SLC12A3 gene encoding the Na-Cl co-transporter (NCCT), the target of thiazide diuretics. The defective function of the NCCT, which normally is expressed in the apical membrane of the distal convolute tubule in the kidney, leads to mild hypotension, hypokalemia, hyperreninemic hyperaldosteronism, mild metabolic alkalosis, hypomagnesemia and hypocalciuria. Up to now, more than 100 mutations of the SLC12A3 gene have been described in GS patients. METHODS: We have collected 30 patients from Sweden with a clinical diagnosis of GS and undertaken a mutation screening by SSCP and successive sequencing of the 26 exons and intronic boundaries. Both mutations were identified in most (n = 28, 93%) and at least one mutation was identified in all patients. RESULTS: We found 22 different mutations evenly distributed throughout the gene, 11 of which have not been described previously. The new variants include 8 missense mutations (Glu68Lys, His69Asn, Argl45His, Vall53Met, Gly230Asp, Gly342Ala, Val677Leu and Gly867Ser), 1 insertion (c.834_835insG on exon 6) and 2 splice-site mutations (c.2667 + lT>G substitution in splicing donor site after exon 22, c.1569-1G>A substitution in the splicing acceptor site before exon 13). CONCLUSION: In Swedish patients with the clinical features of GS, disease-causing mutations in the SLC12A3 gene were identified in most patients. The spectrum of GS mutations is wide making full mutation screening of the SLC12A3 gene necessary to confirm the diagnosis.
  •  
11.
  • Fava, Cristiano, et al. (författare)
  • Subjects heterozygous for genetic loss of function of the thiazide-sensitive cotransporter have reduced blood pressure
  • 2008
  • Ingår i: Human Molecular Genetics. - : Oxford University Press (OUP). - 0964-6906 .- 1460-2083. ; 17:3, s. 413-418
  • Tidskriftsartikel (refereegranskat)abstract
    • Gitelmans syndrome (GS) is an inherited recessive disorder caused by homozygous or compound heterozygous loss of function mutations of the NaCl cotransporter (NCCT) gene encoding the kidney-expressed NCCT, the pharmacological target of thiazide diuretics. An observational study estimated the prevalence of GS to 19/1 000 000, in Sweden, suggesting that similar to 1% of the population carries one mutant NCCT allele. As the phenotype of GS patients, who always carry two mutant alleles, is indistinguishable from that seen in patients treated with high-dose thiazide diuretics, we aimed at investigating whether subjects carrying one mutated NCCT allele have a phenotype resembling that of treatment with low-dose thiazide diuretics. We screened first-degree relatives of 18 of our patients with an established clinical end genetic diagnosis of GS for NCCT loss of function mutations and identified 35 healthy subjects carrying one mutant allele (GS-heterozygotes). Each GS-heterozygote was assigned a healthy control subject matched for age, BMI and sex. GS-heterozygotes had markedly lower blood pressure (systolic 103.3 +/- 16.4 versus 123.2 +/- 19.4 mmHg; diastolic 62.5 +/- 10.5 versus 73.1 +/- 9.4 mmHg; P < 0.001) than controls. There was no significant difference between the groups either in plasma concentration or urinary excretion rate of electrolytes, however, GS-heterozygotes had higher fasting plasma glucose concentration. Similar to patients being treated with low-dose thiazide diuretics, GS-heterozygotes have markedly lower blood pressure and slightly higher fasting plasma glucose compared with control subjects. Our findings suggest that GS-heterozygotes, the prevalence of which can be estimated to 1%, are partially protected from hypertension through partial genetic loss of function of the NCCT. However, as our study had a case-control design, it is important to underline that any potential effects on population blood pressure and risk of future cardiovascular disease need to be examined in prospective and population-based studies.
  •  
12.
  • Fritzell, Peter, et al. (författare)
  • Cost-effectiveness of lumbar fusion and nonsurgical treatment for chronic low back pain in the Swedish lumbar spine study : A multicenter, randomized, controlled trial from the Swedish Lumbar Spine Study Group
  • 2004
  • Ingår i: Spine. - : Lippincott Williams & Wilkins. - 0362-2436 .- 1528-1159. ; 29:4, s. 421-434
  • Tidskriftsartikel (refereegranskat)abstract
    • Study Design. A cost-effectiveness study was performed from the societal and health care perspectives. Objective. To evaluate the costs-effectiveness of lumbar fusion for chronic low back pain (CLBP) during a 2-year follow-up. Summary of Background Data. A full economic evaluation comparing costs related to treatment effects in patients with CLBP is lacking. Patients and Methods. A total of 284 of 294 patients with CLBP for at least 2 years were randomized to either lumbar fusion or a nonsurgical control group. Costs for the health care sector ( direct costs), and costs associated with production losses ( indirect costs) were calculated. Societal total costs were identified as the sum of direct and indirect costs. Treatment effects were measured using patient global assessment of improvement, back pain ( VAS), functional disability (Owestry), and return to work. Results. The societal total cost per patient ( standard deviations) in the surgical group was significantly higher than in the nonsurgical group: Swedish kroner (SEK) 704,000 ( 254,000) vs. SEK 636,000 ( 208,000). The cost per patient for the health care sector was significantly higher for the surgical group, SEK 123,000 ( 60,100) vs. 65,200 ( 38,400) for the control group. All treatment effects were significantly better after surgery. The incremental cost-effectiveness ratio ( ICER), illustrating the extra cost per extra effect unit gained by using fusion instead of nonsurgical treatment, were for improvement: SEK 2,600 ( 600 - 5,900), for back pain: SEK 5,200 ( 1,100 - 11,500), for Oswestry: SEK 11,300 ( 1,200 - 48,000), and for return to work: SEK 4,100 ( 100 21,400). Conclusion. For both the society and the health care sectors, the 2-year costs for lumbar fusion was significantly higher compared with nonsurgical treatment but all treatment effects were significantly in favor of surgery. The probability of lumbar fusion being cost-effective increased with the value put on extra effect units gained by using surgery.
  •  
13.
  •  
14.
  • Gaulton, Kyle J, et al. (författare)
  • Genetic fine mapping and genomic annotation defines causal mechanisms at type 2 diabetes susceptibility loci.
  • 2015
  • Ingår i: Nature Genetics. - : Springer Science and Business Media LLC. - 1546-1718 .- 1061-4036. ; 47:12, s. 1415-1415
  • Tidskriftsartikel (refereegranskat)abstract
    • We performed fine mapping of 39 established type 2 diabetes (T2D) loci in 27,206 cases and 57,574 controls of European ancestry. We identified 49 distinct association signals at these loci, including five mapping in or near KCNQ1. 'Credible sets' of the variants most likely to drive each distinct signal mapped predominantly to noncoding sequence, implying that association with T2D is mediated through gene regulation. Credible set variants were enriched for overlap with FOXA2 chromatin immunoprecipitation binding sites in human islet and liver cells, including at MTNR1B, where fine mapping implicated rs10830963 as driving T2D association. We confirmed that the T2D risk allele for this SNP increases FOXA2-bound enhancer activity in islet- and liver-derived cells. We observed allele-specific differences in NEUROD1 binding in islet-derived cells, consistent with evidence that the T2D risk allele increases islet MTNR1B expression. Our study demonstrates how integration of genetic and genomic information can define molecular mechanisms through which variants underlying association signals exert their effects on disease.
  •  
15.
  • Glimskär, Anders, et al. (författare)
  • Inventering och utveckling för uppföljning av gräsmarks- och hällmarksnaturtyper 2017
  • 2018
  • Rapport (övrigt vetenskapligt/konstnärligt)abstract
    •  Rapporten beskriver resultat från biogeografisk uppföljning av några naturtyper med sparsam förekomst i landskapet. För hällmarkstorrängar (naturtyp 8230) visas resultat från tredje året av uppföljningen, som bland annat visar att en stor andel av hällmarkstorrängarna ligger utanför skyddade områden. För alvar, basiska berghällar och karsthällmark är 2017 det första året för ett förtätat stickprov på Öland och Gotland. Förutom variabler för artförekomst och vegetations­struktur ingår också beskrivning av vittringsmaterial och karstsprickor samt en särskild metodik för karstlevande snäckor. Svämängar har karterats i sin helhet längs Vindelälven, med ett stickprov av fältbesökta ytor. De kommande fem åren är planen att svämängar vid samtliga större vattendrag från Dalälven och norrut karteras. Förutom uppföljningen av dessa naturtyper, så har även utvecklingsinsatser gjorts för andra naturtyper, som underlag för framtida uppföljning. Ett mindre antal objekt av höglänta slåtterängar och slåtterängar i låglandet har inventerats, för att ge underlag för hur artsammansättningen kan ge stöd för en tillförlitlig klassning av slåtter- och betespräglade naturtyper. Första tester har gjorts för hur högörtängar och svämängar kan följas vid mindre vattendrag, men fler år behövs för att slutsatserna ska bli tydliga. Tester har också gjorts för hur Remiils miljöövervakning i gräsmarker kan bidra för vanliga gräsmarks­naturtyper, men en bra lösning förutsätter kraftigt förbättrad samordning mellan olika inventeringar.
  •  
16.
  • Guey, Lin T., et al. (författare)
  • Power in the Phenotypic Extremes: A Simulation Study of Power in Discovery and Replication of Rare Variants
  • 2011
  • Ingår i: Genetic Epidemiology. - : Wiley. - 0741-0395. ; 35:4, s. 236-246
  • Tidskriftsartikel (refereegranskat)abstract
    • Next-generation sequencing technologies are making it possible to study the role of rare variants in human disease. Many studies balance statistical power with cost-effectiveness by (a) sampling from phenotypic extremes and (b) utilizing a two-stage design. Two-stage designs include a broad-based discovery phase and selection of a subset of potential causal genes/variants to be further examined in independent samples. We evaluate three parameters: first, the gain in statistical power due to extreme sampling to discover causal variants; second, the informativeness of initial (Phase I) association statistics to select genes/variants for follow-up; third, the impact of extreme and random sampling in (Phase 2) replication. We present a quantitative method to select individuals from the phenotypic extremes of a binary trait, and simulate disease association studies under a variety of sample sizes and sampling schemes. First, we find that while studies sampling from extremes have excellent power to discover rare variants, they have limited power to associate them to phenotype-suggesting high false-negative rates for upcoming studies. Second, consistent with previous studies, we find that the effect sizes estimated in these studies are expected to be systematically larger compared with the overall population effect size; in a well-cited lipids study, we estimate the reported effect to be twofold larger. Third, replication studies require large samples from the general population to have sufficient power; extreme sampling could reduce the required sample size as much as fourfold. Our observations offer practical guidance for the design and interpretation of studies that utilize extreme sampling. Genet. Epidemiol. 35: 236-246, 2011. (c) 2011 Wiley-Liss, Inc.
  •  
17.
  • Hertel, Jens K., et al. (författare)
  • FTO, Type 2 Diabetes, and Weight Gain Throughout Adult Life A Meta-Analysis of 41,504 Subjects From the Scandinavian HUNT, MDC, and MPP Studies
  • 2011
  • Ingår i: Diabetes. - : American Diabetes Association. - 1939-327X .- 0012-1797. ; 60:5, s. 1637-1644
  • Tidskriftsartikel (refereegranskat)abstract
    • OBJECTIVE-FTO is the most important polygene identified for obesity. We aimed to investigate whether a variant in FTO affects type 2 diabetes risk entirely through its effect on BMI and how FTO) influences BMI across adult life span. RESEARCH DESIGN AND METHODS-Through regression models, we assessed the relationship between the FTO single nucleotide polymorphisms rs9939609, type 2 diabetes, and BMI across life span in subjects from the Norwegian population-based HUNT study using cross-sectional and longitudinal perspectives. For replication and meta-analysis, we used data from the Malmo Diet and Cancer (MDC) and Malmo Preventive Project (MPP) cohorts, comprising a total sample of 41,504 Scandinavians. RESULTS-The meta-analysis revealed a highly significant association for rs9939609 with both type 2 diabetes (OR 1.13; P = 4.5 x 10(-8)) and the risk to develop incident type 2 diabetes (OR 1.16; P = 3.2 x 10(-8)). The associations remained also after correction for BMI and other anthropometric measures. Furthermore, we confirmed the strong effect on BMI (0.28 kg/m(2) per risk allele; P = 2.0 x 10(-26), with no heterogeneity between different age-groups. We found no differences in change of BMI over time according to rs9939609 risk alleles, neither overall (Delta BMI = 0.0 [-0.05, 0.05]) nor in any individual age stratum, indicating no further weight gain attributable to FTO genotype in adults. CONCLUSIONS-We have identified that a variant in FTO alters type 2 diabetes risk partly independent of its observed effect on BMI. The additional weight gain as a result of the FTO risk variant seems to occur before adulthood, and the BMI difference remains stable thereafter. Diabetes 60:1637-1644, 2011
  •  
18.
  • Heydorn, Per, et al. (författare)
  • Long-term rearing affects pheromone-mediated flight behaviour of the Indian meal moth, Plodia interpunctella
  • 2019
  • Ingår i: Journal of Applied Entomology. - : Wiley. - 0931-2048 .- 1439-0418. ; 143:10, s. 1193-1195
  • Tidskriftsartikel (refereegranskat)abstract
    • The pest Plodia interpunctella (Hübner) is reared in many research laboratories. In a culture established in 1996, attraction of males to the female-produced sex pheromone in flight tunnel assays gradually decreased after ≈15 years of rearing. A new culture was established to enable comparison with the old culture regarding traits associated with mate finding. Female calling activity, pheromone titre and male antennal response to pheromone components did not differ between cultures. In contrast, very few males from the old culture reached the pheromone source in flight tunnel assays compared with 61%–81% of males from the other culture. Our results highlight the importance of maintaining viable insect cultures for research purposes and suggest frequent evaluation of traits involved in chemical communication in such cultures to ensure reliable results in experiments.
  •  
19.
  • Hossain, Mohammad Moshin (författare)
  • Effects of HCO3- and ionic strength on the oxidation and dissolution of UO2
  • 2006
  • Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • The kinetics for radiation induced dissolution of spent nuclear fuel is a key issue in the safety assessment of a future deep repository. Spent nuclear fuel mainly consists of UO2 and therefore the release of radionuclides (fission products and actinides) is assumed to be governed by the oxidation and subsequent dissolution of the UO2 matrix. The process is influenced by the dose rate in the surrounding groundwater (a function of fuel age and burn up) and on the groundwater composition. In this licentiate thesis the effects of HCO3- (a strong complexing agent for UO22+) and ionic strength on the kinetics of UO2 oxidation and dissolution of oxidized UO2 have been studied experimentally. The experiments were performed using aqueous UO2 particle suspensions where the oxidant concentration was monitored as a function of reaction time. These reaction systems frequently display first order kinetics. Second order rate constants were obtained by varying the solid UO2 surface area to solution volume ratio and plotting the resulting pseudo first order rate constants against the surface area to solution volume ratio. The oxidants used were H2O2 (the most important oxidant under deep repository conditions), MnO4- and IrCl62-. The kinetics was studied as a function of HCO3- concentration and ionic strength (using NaCl and Na2SO4 as electrolytes). The rate constant for the reaction between H2O2 and UO2 was found to increase linearly with the HCO3- concentration in the range 0-1 mM. Above 1 mM the rate constant is independent of the HCO3- concentration. The HCO3- concentration independent rate constant is interpreted as being the true rate constant for oxidation of UO2 by H2O2 [(4.4 ± 0.3) x 10-6 m min-1] while the HCO3- concentration dependent rate constant is used to estimate the rate constant for HCO3- facilitated dissolution of UO22+ (oxidized UO2) [(8.8 ± 0.5) x 10-3 m min-1]. From experiments performed in suspensions free from HCO3- the rate constant for dissolution of UO22+ was also determined [(7 ± 1) x 10-8 mol m-2 s-1]. These rate constants are of significant importance for simulation of spent nuclear fuel dissolution. The rate constant for the oxidation of UO2 by H2O2 (the HCO3- concentration independent rate constant) was found to be independent of ionic strength. However, the rate constant for dissolution of oxidized UO2 displayed ionic strength dependence, namely it increases with increasing ionic strength. The HCO3- concentration and ionic strength dependence for the anionic oxidants is more complex since also the electron transfer process is expected to be ionic strength dependent. Furthermore, the kinetics for the anionic oxidants is more pH sensitive. For both MnO4- and IrCl62- the rate constant for the reaction with UO2 was found to be diffusion controlled at higher HCO3- concentrations (~0.2 M). Both oxidants also displayed ionic strength dependence even though the HCO3- independent reaction could not be studied exclusively. Based on changes in reaction order from first to zeroth order kinetics (which occurs when the UO2 surface is completely oxidized) in HCO3- deficient systems the oxidation site density of the UO2 powder was determined. H2O2 and IrCl62- were used in these experiments giving similar results [(2.1 ± 0.1) x 10-4 and (2.7 ± 0.5) x 10-4 mol m-2, respectively].
  •  
20.
  • Hulsart Billström, Gry, 1982- (författare)
  • Bone Regeneration with Cell-free Injectable Scaffolds
  • 2017
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Bone is a remarkable multifunctional tissue with the ability to regenerate and remodel without generating any scar tissue. However, bone loss due to injury or diseases can be a great challenge and affect the patient significantly. Autologous bone grafting is commonly used throughout the world. Autograft both fills the void and is bone inductive, housing the particular cells that are needed for bone regeneration. However, a regenerative complement to autograft is of great interest as the use of biomaterials loaded with bioactive molecules can avoid donor site morbidity and the problem of a limited volume of material. Two such regenerative products that utilise bone morphogenetic protein (BMP)-7 and -2 have been used for more than a decade clinically. Unfortunately, several side effects have been reported, such as severe swelling due to inflammation and ectopic bone formation. Additionally, the products require open surgery and use of supra physiological doses of the BMPs due to poor localisation and retention of the growth factor. The purpose of this thesis was to harness the strong inductive capacity of the BMP-2 by optimising the carrier of this bioactive protein, thereby minimising the side effects that are associated with the clinical products and facilitating safe and localised bone regeneration. We focused on an injectable hyaluronan-based carrier developed through polymer chemistry at the University of Uppsala. The strategy was to use the body’s own regenerative pathway to stimulate and enhance bone healing in a manner similar to the natural bone-healing process. The hyaluronan-based carrier has a similar composition to the natural extracellular matrix and is degraded by resident enzymes. Earlier studies have shown improved properties when adding hydroxyapatite, a calcium phosphate that constitutes the inorganic part of the bone matrix. In Paper I, the aim was to improve the carrier by adding other forms of calcium phosphate. The results indicated that bone formation was enhanced when using nano-sized hydroxyapatite. In Paper II, we discovered the importance of crushing the material, thus enhancing permeability and enlarging the surface area. We wished to further develop the carrier system, but were lacking an animal model with relatively high throughput, facilitated access, paired data, and we were also committed to the 3Rs of refinement, reduction, and replacement. To meet these challenges, we developed and refined an animal model, and this is described in Paper III. In Paper IV, we sought to further optimise the biomaterial properties of the hydrogel through covalent bonding of bisphosphonates to the hyaluronan hydrogel. This resulted in exceptional retention of the growth factor BMP-2. In Paper V, SPECT/PET/µCT was combined as a tri-modal imaging method to allow visualisation of the biomaterial’s in situ action, in terms of drug retention, osteoblast activity and mineralisation. Finally, in Paper VI the correlation between existing in vitro results with in vivo outcomes was observed for an array of biomaterials. The study identified a surprisingly poor correlation between in vitro and in vivo assessment of biomaterials for osteogenesis.
  •  
21.
  • Johnsson, Martin, et al. (författare)
  • A sexual ornament in chickens is affected by pleiotropic alleles at HAO1 and BMP2, selected during domestication.
  • 2012
  • Ingår i: PLoS genetics. - : Public Library of Science (PLoS). - 1553-7404 .- 1553-7390. ; 8:8
  • Tidskriftsartikel (refereegranskat)abstract
    • Domestication is one of the strongest forms of short-term, directional selection. Although selection is typically only exerted on one or a few target traits, domestication can lead to numerous changes in many seemingly unrelated phenotypes. It is unknown whether such correlated responses are due to pleiotropy or linkage between separate genetic architectures. Using three separate intercrosses between wild and domestic chickens, a locus affecting comb mass (a sexual ornament in the chicken) and several fitness traits (primarily medullary bone allocation and fecundity) was identified. This locus contains two tightly-linked genes, BMP2 and HAO1, which together produce the range of pleiotropic effects seen. This study demonstrates the importance of pleiotropy (or extremely close linkage) in domestication. The nature of this pleiotropy also provides insights into how this sexual ornament could be maintained in wild populations.
  •  
22.
  • Johnsson, Martin, et al. (författare)
  • The role of pleiotropy and linkage in genes affecting a sexual ornament and bone allocation in the chicken.
  • 2014
  • Ingår i: Molecular ecology. - : Wiley. - 1365-294X .- 0962-1083. ; 23:9, s. 2275-86
  • Tidskriftsartikel (refereegranskat)abstract
    • Sexual selection and the ornaments that inform such choices have been extensively studied, particularly from a phenotypic perspective. Although more is being revealed about the genetic architecture of sexual ornaments, much still remains to be discovered. The comb of the chicken is one of the most widely recognized sexual ornaments, which has been shown to be correlated with both fecundity and bone allocation. In this study, we use a combination of multiple intercrosses between White Leghorn populations and wild-derived Red Junglefowl to, first, map quantitative trait loci (QTL) for bone allocation and, second, to identify expression QTL that correlate and colocalize with comb mass. These candidate quantitative genes were then assessed for potential pleiotropic effects on bone tissue and fecundity traits. We identify genes that correlate with both relative comb mass and bone traits suggesting a combination of both pleiotropy and linkage mediates gene regulatory variation in these traits.
  •  
23.
  • Jonsson, Alexander, et al. (författare)
  • Protein Kinase R Is Constitutively Expressed in the Human Pancreas
  • 2019
  • Ingår i: Journal of Histochemistry & Cytochemistry. - : SAGE Publications. - 0022-1554 .- 1551-5044. ; 67:2, s. 99-105
  • Tidskriftsartikel (refereegranskat)abstract
    • Viral infection of the insulin-producing cells in the pancreas has been proposed in the etiology of type 1 diabetes. Protein kinase R (PKR) is a cytoplasmic protein activated through phosphorylation in response to cellular stress and particularly viral infection. As PKR expression in pancreatic beta-cells has been interpreted as a viral footprint, this cross-sectional study aimed at characterizing the PKR expression in non-diabetic human pancreases. PKR expression was evaluated in pancreas tissue from 16 non-diabetic organ donors, using immunohistochemistry, qPCR, and western blot. Immunohistochemistry and western blot showed readily detectable PKR expression in the pancreatic parenchyma. The qPCR detected PKR mRNA in both endocrine and exocrine samples, with a slightly higher expression in the islets. In conclusion, PKR is constitutively expressed in both endocrine and exocrine parts of the pancreas and its expression should not be interpreted as a viral footprint in pancreatic beta cells.
  •  
24.
  • Jonsson, Alexander, et al. (författare)
  • Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism
  • 2020
  • Ingår i: Scientific Reports. - BERLIN GERMANY : Springer Science and Business Media LLC. - 2045-2322. ; 10:1
  • Tidskriftsartikel (refereegranskat)abstract
    • In experimental studies, pancreatic islet microvasculature is essential for islet endocrine function and mass, and islet vascular morphology is altered in diabetic subjects. Even so, almost no information is available concerning human islet microvascular endothelial cell (MVEC) physiology and gene expression. In this study, islets and exocrine pancreatic tissue were acquired from organ donors with normoglycemia or impaired glucose metabolism (IGM) immediately after islet isolation. Following single-cell dissociation, primary islet- and exocrine MVECs were obtained through fluorescence-activated cell sorting (FACS) and transcriptional profiles were generated using AmpliSeq. Multiple gene sets involved in general vascular development and extracellular matrix remodeling were enriched in islet MVEC. In exocrine MVEC samples, multiple enriched gene sets that relate to biosynthesis and biomolecule catabolism were found. No statistically significant enrichment was found in gene sets related to autophagy or endoplasmic reticulum (ER) stress. Although ample differences were found between islet- and exocrine tissue endothelial cells, no differences could be observed between normoglycemic donors and donors with IGM at gene or gene set level. Our data is consistent with active angiogenesis and vascular remodeling in human islets and support the notion of ongoing endocrine pancreas tissue repair and regeneration even in the adult human.
  •  
25.
  • Jonsson, Emma, et al. (författare)
  • Cost and Health Outcomes Patterns in Patients Treated With Spinal Cord Stimulation Following Spine Surgery : A Register-Based Study
  • 2020
  • Ingår i: Neuromodulation. - : Elsevier BV. - 1094-7159 .- 1525-1403. ; 23:5, s. 626-633
  • Tidskriftsartikel (refereegranskat)abstract
    • ObjectiveSpinal cord stimulation (SCS), a minimally invasive treatment option for long‐term neuropathic pain, has been shown to be effective in patients with persisting neuropathic pain after spine surgery. However, little is known about the long‐term cost and quality‐of‐life (QoL) patterns in SCS‐treated patients. The aim is to describe the use of SCS, costs, pre‐spine‐surgery and post‐spine‐surgery QoL, and reported pain intensity, in patients who have undergone spine surgery and subsequent SCS implantation. The results will be related to outcome and cost in spine surgery patients in general.Materials and MethodsA research database comprised from six Swedish national and regional registers, and the spine surgery quality‐of‐care register Swespine was utilized. Two cohorts were identified: all patients who had spine surgery (N = 73,765) and patients who had spine surgery and subsequent SCS implantation (N = 239). Costs were analyzed before and after spine surgery for both cohorts, as well as before and after SCS implantation for the second cohort. QoL was explored by estimating patient‐reported outcome measures such as pain intensity, Oswestry Disability Index, and EuroQol‐5Dimensions from spine surgery up to five years post–spine surgery.ResultsIn spine surgery patients, mean QoL and pain intensity levels improved following surgery. Patients subsequently treated with SCS had lower reported QoL and higher costs before the initial spine surgery, and spine surgery did not lead to any substantial improvements, however, costs decreased following SCS implantation in these patients.
  •  
26.
  •  
27.
  • Jonsson, Fredrik, et al. (författare)
  • Ringlavsövervakning i Gävleborg 1996-2002
  • 2003
  • Rapport (övrigt vetenskapligt/konstnärligt)abstract
    • 1996 och 1997 startades en övervakning av 19 ringlavslokaler i Gävlebors län. I denna rapport redovisas resultatet från en återinventering av dessa lokaler år 2002. I rapporten redovisas också de förändringar som skett på lokaler år 2002. i rapporten redovisas också de förändringar som skett på lokalerna under perioden 1996 till 2002.Inventeringen skedde på fasta provytor, 10 x 20 m stora (provytekartering) och längs transekter utgående från dessa (grovkartering).I Hälsingland har 14 lokaler inventerats.På sex av lokalerna har antalet ringlavsbålar ökat kraftigt, mer än 40% i provytorna sedan inventeringsstarten. På ytterligare fyra har en måttligare ökning skett och på fyra har en minskning skett.I Gästrikland har fem lokaler övervakats och där har ringlaven ökat kraftigt på en lokal i norra Gästrikland medan den har minskat mer eller mindre kraftigt på de fyra som ligger i den södra halvan av landskapet.I Gästrikland växte ringlaven i lika stor utsträckning på gran som på en, medan ringlaven i Hälsingland växte främst på gran, men även ganska rikligt på tall och björk.på samtliga ringlavslokaler söder om gävle i gästrikland har ringlaven minskat kraftigt. Detta trots att inga stora förändringar tycks ha skett i provytornas omedelbara närhet sedan inventeringsstarten.I Hälsningland och norra gästrikland förfaller bestånden vara relativt stabila.En viktig del i skyddet av ringlav i Gävleborgs län är att genom inventeringsinsater finna och avgränsa hittils okända ringlavslokaler.det är troligt att flera oupptäckta ringlavslokaler avverkas på grund av att man saknar kännedom om dem.
  •  
28.
  • Jonsson, Fredrik, et al. (författare)
  • Övervakning av varglav i Gävleborg 1996-2001
  • 2002
  • Rapport (övrigt vetenskapligt/konstnärligt)abstract
    • År 1996 valdes 20 lokaler, varav 16 är myrlokaler och 4 fastmarkslokaler, med känd förekomst av varglav ut och inventerades. År 2001 återinventerades samtliga av dessa lokaler.På varje lokal gjordes en provytekartering,detaljkartering och grovkartering av varglav.Sammanfattningsvis av inventeringen kan sägas att de flesta myrlokaler hyser så stora och stabila populationer att de ej är hotade på kortsikt.För flera av fastmarkslokalerna är framtiden mer osäker.Dessa lokaler hyser endast några få bålar på enstaka träd och nyetableringen är otillfredställande.
  •  
29.
  • Jonsson, Ing-Marie, 1960- (författare)
  • Social and Emotional Characteristics of Speech-based In-Vehicle Information Systems : Impact on Attitude and Driving Behaviour
  • 2009
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Modern vehicles use advanced information systems in vehicles to provide and control a wide variety of functions and features. Even modest vehicles today are equipped with systems that control diverse functions from air-conditioning to high quality audio/video systems.Since driving requires the use of eyes and hands, voice interaction has become more widely used by in-vehicle systems. Due to the technical complexity involved in voice recognition, focus has been on issues of speech ecognition. Speech generation is comparatively simple, but what effect does the choice of voice have on the driver? We know from human-human interaction that social cues of the voice itself influence attitude and interpretation of information. Introducing speech based communication with the car changes the relationship between driver and vehicle. So, for in-vehicle information systems, does the spoken voice matter?The work presented in this thesis studies the effects of the voice used by invehicle systems. A series of studies were used to answer the following questions: Do the characteristics of voices used by an in-vehicle system affect driver’s attitude? Do the characteristics of voice used by an in-vehicle system affect driver’s performance? Are social reactions to voice communication the same in the car environment as in the office environment?Results show that voices do matter! Voices trigger social and emotional effects that impact both attitude and driving performance. Moreover, there is not one effective voice that works for all drivers. Therefore an in-vehicle system that knows its driver and possibly adapts to its driver can be the most effective. Finally, an interesting observation from these studies is that social reactions to voice communication in the car are different than in the office, Similarity attraction, an otherwise solid finding in social science, did not hold all studies. It is hypothesized that this difference can be related to the different kinds of task demands when driving a car or working in an office environment.
  •  
30.
  •  
31.
  • Jonsson, Magnus H., et al. (författare)
  • Novel biomarkers for prediction of outcome in hip fracture patients—An exploratory study
  • 2020
  • Ingår i: Acta Anaesthesiologica Scandinavica. - : Wiley. - 0001-5172 .- 1399-6576. ; 64:7, s. 920-927
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Little is known about the value of biomarkers for prognostication in hip fracture patients. The main objective of the present study was to assess if biomarkers add useful information to an existing risk score for prediction of 30-day mortality in patients suffering from out of hospital hip fractures. Methods: In a prospective observational single centre study, association between plasma concentration of ninety-two biomarkers at admission and 30-day mortality was analysed using logistic regression adjusted for risk factors included in Nottingham Hip Fracture Score (NHFS). Biomarkers associated with the outcome in the adjusted analysis were further evaluated by calculating the net reclassification improvement (NRI) and the change in area under the receiver operating characteristics curve (AUC) relative to the NHFS. Results: 997 patients were included. Sixty-two patients died within 30 days (6.2%). Eleven biomarkers were associated with 30-day mortality in adjusted analysis. Of these biomarkers Growth Differentiation Factor-15 (GDF-15) had NRI for the primary outcome (12.1%; 95% CI: 1.2-23.3) and Carbohydrate Antigen 125 (CA-125) improved the AUC relative to NHFS (improvement: 0.05; 95% CI: 0.01-0.10, P =.027). Both CA-125 and GDF-15 improved the AUC for a composite outcome of 30-day mortality and cardiovascular complications. Conclusions: Adding GDF-15 or CA-125 to the Nottingham Hip Fracture Score improves the discrimination with regard to predicting 30-day mortality and may help to identify a subgroup of hip fracture patients with a particularly poor prognosis. The value of these biomarkers should be explored in further studies to confirm clinical utility.
  •  
32.
  • Jonsson, Magnus H., et al. (författare)
  • Plasma lactate at admission does not predict mortality and complications in hip fracture patients : a prospective observational study
  • 2018
  • Ingår i: Scandinavian Journal of Clinical and Laboratory Investigation. - : Informa UK Limited. - 0036-5513 .- 1502-7686. ; 78:6, s. 508-514
  • Tidskriftsartikel (refereegranskat)abstract
    • Hip fractures in elderly carry a high mortality. Our objective was to test the hypothesis that plasma lactate concentration at hospital admission can be used to identify patients with a high risk for poor outcome. Hip fracture patients admitted to a university hospital in Sweden from January 2011 to August 2014 in whom a venous lactate was obtained at admission were included in this prospective observational study. Primary outcome measure was 30-d mortality and secondary outcome measure was a composite outcome of 30-d mortality and postoperative complications. Lactate concentration was evaluated as a continuous predictor using logistic regression, crude and adjusted for age, gender and American Society of Anesthesiology Physical Status (ASA PS) score. Discrimination was evaluated using receiver operating characteristics (ROC) analysis. Totally, 690 patients were included. Median age was 84 years (interquartile range [IQR] 77–90). At 30-d follow-up, mortality was 7.2%, and 45% of the patients had suffered the composite outcome. Median lactate level was 1.3 mmol/L (IQR 1.0–1.8 mmol/L). The odds ratio (OR) by each 1.0 mmol/L increase in the lactate concentration for 30-d mortality was 1.13 (95% CI 0.77–1.68) while for the composite outcome it was 1.06 (95% CI 0.85–1.3). Similar results were obtained after adjustment for age, sex and ASA PS classification for both outcomes. Area under the ROC curve for lactate as a predictor of 30-d mortality was 0.51 (95% CI 0.45–0.57). In our cohort, plasma lactate at admission does not appear to be a useful biomarker to identify high-risk patients after hip fracture.
  •  
33.
  • Jonsson, Olle (författare)
  • Biogeografisk uppföljning av grynsnäckor: Startinventering med etablering av uppföljningsstationer 2013-2018
  • 2019
  • Rapport (övrigt vetenskapligt/konstnärligt)abstract
    • Denna rapport sammanfattar läget i den biogeografiska uppföljningen av grynsnäckor i Sverige. Uppföljningsarterna ingår i EU:s Art- och habitatdirektiv, bilaga 2, och deras bevarandestatus ska rapporteras till EU vart sjätte år. Efter fältinventeringar 2013-2018 är startdrevet i uppföljningen fullbordat och referensvärden för uppföljningsarterna finns att utgå från vid utvärdering av framtida uppföljningsomdrev. Totalt har 112 uppföljningsstationer etablerats och inventerats en gång, i rikkärr spridda över arternas utbredningsområden i landet. Bland dessa utgör 80 stationer för Vertigo geyeri och 70 stationer för V. genesii, båda arter rödlistade (nära hotade). Dessa arter har mycket likartade livsmiljökrav och till stora delar överlappande utbredningsområden. Ibland ingår bägge arter på samma uppföljningsstation. I huvudsak görs uppföljning av kända lokaler, men en mindre andel okända lokaler ingår. Vertigo angustior (ej rödlistad), som är vanlig på Öland och Gotland, är också en art för vilken bevarandestatus ska rapporteras till EU vart sjätte år. Arten ingår extensivt i uppföljningen via bifångstdata från uppföljningsstationer där den lever tillsammans med V. geyeri eller V. genesii och därför kan påträffas.
  •  
34.
  • Jonsson, Olle (författare)
  • Genetic variation, clonal diversity and breeding system in sedges (Carex)
  • 1998
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • In this thesis I investigate genetic variation in clonal plants within the wind-pollinated plant family Cyperaceae and, in particular, in species of Carex. Clonal diversity and the spatial distribution of clones (genets) within populations was investigated in Carex bigelowii (in Iceland and Scandinavia) and C. arenaria (within the species' entire distributional range in W Europe). Both species reproduce mainly by vegetative growth, via rhizomes with long internodes between shoots, and seedling recruitment in established populations is extremely rare or absent. The C. bigelowii populations show high levels of clonal diversity but Ð contrary to predictions based on the extensive clone structure Ð little interdigitation of genets within local patches. Carex arenaria populations are generally multiclonal, but there appear to be some monoclonal populations in SW Europe. A literature survey of of allozyme data in species of Cyperaceae revealed a strong correlation between clonal growth form and plant breeding system. While caespitose species are generally inbreeding and show little within-population variation, rhizomatous species are outcrossing and have high levels of within population variation. Allozyme investigations of the rhizomatous sedges Carex arenaria, C. bigelowii, C. caryophyllea and C. ericetorum, suggest that these four species are all outbreeders. Carex arenaria, has, however, unusually low overall levels of genetic variation for a rhizomatous sedge. This lack of variation was interpreted in terms of the species' history of glacial survival and postglacial spread. The species-pair C. caryophyllea and C. ericetorum was investigated for ecological amplitude and niche separation in mixed populations in Alvar grassland communities. The two species showed an almost complete overlap in habitat niche, but had partially separate flowering periods. There was a phenological window for pollen transfer from C. caryophyllea to C. ericetorum and allozyme data suggest that there may be gene flow from C. caryophyllea to C. ericetorum.
  •  
35.
  •  
36.
  • Jonsson, Viktor, 1987, et al. (författare)
  • Modelling of zero-inflation improves inference of metagenomic gene count data
  • 2019
  • Ingår i: Statistical Methods in Medical Research. - : SAGE Publications. - 0962-2802 .- 1477-0334. ; 28:12, s. 3712-3728
  • Tidskriftsartikel (refereegranskat)abstract
    • Metagenomics enables the study of gene abundances in complex mixtures of microorganisms and has become a standard methodology for the analysis of the human microbiome. However, gene abundance data is inherently noisy and contains high levels of biological and technical variability as well as an excess of zeros due to non-detected genes. This makes the statistical analysis challenging. In this study, we present a new hierarchical Bayesian model for inference of metagenomic gene abundance data. The model uses a zero-inflated overdispersed Poisson distribution which is able to simultaneously capture the high gene-specific variability as well as zero observations in the data. By analysis of three comprehensive datasets, we show that zero-inflation is common in metagenomic data from the human gut and, if not correctly modelled, it can lead to substantial reductions in statistical power. We also show, by using resampled metagenomic data, that our model has, compared to other methods, a higher and more stable performance for detecting differentially abundant genes. We conclude that proper modelling of the gene-specific variability, including the excess of zeros, is necessary to accurately describe gene abundances in metagenomic data. The proposed model will thus pave the way for new biological insights into the structure of microbial communities.
  •  
37.
  • Jonsson, Viktor, et al. (författare)
  • Photoelectron dispersion in metallic and insulating thin films
  • 2021
  • Ingår i: Physical Review Research. - : American Physical Society. - 2643-1564. ; 3:3
  • Tidskriftsartikel (refereegranskat)abstract
    • The underlying mechanism behind the metal-to-insulator transition in is still a topic of intense debate. The two leading theoretical interpretations associate the transition with either electron-lattice or electron-electron correlations. Novel experimental results are required to converge towards one of the two scenarios. Here we report on a temperature-dependent angle-resolved photoelectron study of thin films across the metal-to-insulator transition. The obtained experimental results are compared to density functional theory calculations. We find an overall energy shift and compression of the electronic band structure across the transition while the overall band topology is conserved. The results demonstrate the importance of electron-electron correlations in establishing the insulating state.
  •  
38.
  • Jonsson, Viktor, 1987, et al. (författare)
  • Statistical evaluation of methods for identification of differentially abundant genes in comparative metagenomics
  • 2016
  • Ingår i: BMC Genomics. - : Springer Science and Business Media LLC. - 1471-2164. ; 17
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Metagenomics is the study of microbial communities by sequencing of genetic material directly from environmental or clinical samples. The genes present in the metagenomes are quantified by annotating and counting the generated DNA fragments. Identification of differentially abundant genes between metagenomes can provide important information about differences in community structure, diversity and biological function. Metagenomic data is however high-dimensional, contain high levels of biological and technical noise and have typically few biological replicates. The statistical analysis is therefore challenging and many approaches have been suggested to date. Results: In this article we perform a comprehensive evaluation of 14 methods for identification of differentially abundant genes between metagenomes. The methods are compared based on the power to detect differentially abundant genes and their ability to correctly estimate the type I error rate and the false discovery rate. We show that sample size, effect size, and gene abundance greatly affect the performance of all methods. Several of the methods also show non-optimal model assumptions and biased false discovery rate estimates, which can result in too large numbers of false positives. We also demonstrate that the performance of several of the methods differs substantially between metagenomic data sequenced by different technologies. Conclusions: Two methods, primarily designed for the analysis of RNA sequencing data (edgeR and DESeq2) together with a generalized linear model based on an overdispersed Poisson distribution were found to have best overall performance. The results presented in this study may serve as a guide for selecting suitable statistical methods for identification of differentially abundant genes in metagenomes.
  •  
39.
  • Jonsson, Viktor, 1987, et al. (författare)
  • Variability in Metagenomic Count Data and Its Influence on the Identification of Differentially Abundant Genes.
  • 2017
  • Ingår i: Journal of Computational Biology. - : Mary Ann Liebert Inc. - 1066-5277 .- 1557-8666. ; 24:4, s. 311-326
  • Tidskriftsartikel (refereegranskat)abstract
    • Metagenomics is the study of microorganisms in environmental and clinical samples using high-throughput sequencing of random fragments of their DNA. Since metagenomics does not require any prior culturing of isolates, entire microbial communities can be studied directly in their natural state. In metagenomics, the abundance of genes is quantified by sorting and counting the DNA fragments. The resulting count data are high-dimensional and affected by high levels of technical and biological noise that make the statistical analysis challenging. In this article, we introduce an hierarchical overdispersed Poisson model to explore the variability in metagenomic data. By analyzing three comprehensive data sets, we show that the gene-specific variability varies substantially between genes and is dependent on biological function. We also assess the power of identifying differentially abundant genes and show that incorrect assumptions about the gene-specific variability can lead to unacceptable high rates of false positives. Finally, we evaluate shrinkage approaches to improve the variance estimation and show that the prior choice significantly affects the statistical power. The results presented in this study further elucidate the complex variance structure of metagenomic data and provide suggestions for accurate and reliable identification of differentially abundant genes.
  •  
40.
  • Lindstroem, Marianne, et al. (författare)
  • Attitudes towards the Conservation of Biological Diversity - a Case Study in Kristianstad Municipality, Sweden
  • 2006
  • Ingår i: Journal of Environmental Planning and Management. - : Informa UK Limited. - 0964-0568 .- 1360-0559. ; 49:4, s. 495-513
  • Tidskriftsartikel (refereegranskat)abstract
    • Human actions towards land, freshwater and oceans have already caused biodiversity to decline. This study aims to investigate attitudes towards the conservation of biological biodiversity among different groups in a Swedish city, Kristianstad. An inquiry including statements measuring attitudes towards the conservation of habitats, animals and plants, to the biological diversity within selected local areas, to global and national areas, and to societal issues, was replied to by 271 persons. Deciduous forests, birds and wild flowers were given highest priority. An area categorized as wetland including lakeshore meadows with a rich bird life was prioritized as most important for conservation while a forest area was chosen as best for recreation. The experts gave lower priority to arable land, urban parks, domestic animals, agricultural and garden plants and to conifer forests compared to the other groups. Knowledge about what people in general value as important could facilitate the future planning of nature areas in the city of Kristianstad. 
  •  
41.
  • Lundberg, J., et al. (författare)
  • Endovascular Method for Transplantation of Insulin-Producing Cells to the Pancreas Parenchyma in Swine
  • 2014
  • Ingår i: American Journal of Transplantation. - : Elsevier BV. - 1600-6135 .- 1600-6143. ; 14:3, s. 694-700
  • Tidskriftsartikel (refereegranskat)abstract
    • Insulin-producing cells are transplanted by portal vein injection as an alternative to pancreas transplantation in both clinical and preclinical trials. Two of the main limitations of portal vein transplantation are the prompt activation of the innate immunity and concomitant loss of islets and a small but significant risk of portal vein thrombosis. Furthermore, to mimic physiological release, the insulin-producing cells should instead be located in the pancreas. The trans-vessel wall approach is an endovascular method for penetrating the vessel wall from the inside. In essence, a working channel is established to the parenchyma of organs that are difficult to access by percutaneous technique. In this experiment, we accessed the extra-vascular pancreatic parenchyma in swine by microendovascular technique and injected methylene blue, contrast fluids and insulin-producing cells without acute adverse events. Further, we evaluated the procedure itself by a 1-year angiographical follow-up, without adverse events. This study shows that the novel approach utilizing endovascular minimal invasiveness coupled to accurate trans-vessel wall placement of an injection in the pancreatic parenchyma with insulin-producing cells is possible. In clinical practice, the potential benefits compared to portal vein cell transplantation should significantly improve endocrine function of the graft and potentially reduce adverse events. This study presents one-year follow-up safety data on the microendovascular trans-vessel wall technique and shows that the technique can be used to transplant insulin-producing cells to the swine pancreas parenchyma.
  •  
42.
  • Lyssenko, Valeriya, et al. (författare)
  • Pleiotropic Effects of GIP on Islet Function Involve Osteopontin
  • 2011
  • Ingår i: Diabetes. - : American Diabetes Association. - 1939-327X .- 0012-1797. ; 60:9, s. 2424-2433
  • Tidskriftsartikel (refereegranskat)abstract
    • OBJECTIVE-The incretin hormone GIP (glucose-dependent insulinotropic polypeptide) promotes pancreatic beta-cell function by potentiating insulin secretion and beta-cell proliferation. Recently, a combined analysis of several genome-wide association studies (Meta-analysis of Glucose and Insulin-Related Traits Consortium [MAGIC]) showed association to postprandial insulin at the GIP receptor (GIPR) locus. Here we explored mechanisms that could explain the protective effects of GIP on islet function. RESEARCH DESIGN AND METHODS-Associations of GIPR rs10423928 with metabolic and anthropometric phenotypes in both nondiabetic (N = 53,730) and type 2 diabetic individuals (N = 2,731) were explored by combining data from 11 studies.Insulin secretion was measured both in vivo in nondiabetic subjects and in vitro in islets from cadaver donors. Insulin secretion was also measured in response to exogenous GIP. The in vitro measurements included protein and gene expression as well as measurements of beta-cell viability and proliferation. RESULTS-The A allele of GIPR rs10423928 was associated with impaired glucose- and GIP-stimulated insulin secretion and a decrease in BMI, lean body mass, and waist circumference. The decrease in BMI almost completely neutralized the effect of impaired insulin secretion on risk of type 2 diabetes. Expression of GIPR mRNA was decreased in human islets from carriers of the A allele or patients with type 2 diabetes. GIP stimulated osteopontin (OPN) mRNA and protein expression. OPN expression was lower in carriers of the A allele. Both GIP and OPN prevented cytokine-induced reduction in cell viability (apoptosis). In addition, OPN stimulated cell proliferation in insulin-secreting cells. CONCLUSIONS-These findings support beta-cell proliferative and antiapoptotic roles for GIP in addition to its action as an incretin hormone. Identification of a link between GIP and OPN may shed new light on the role of GIP in preservation of functional beta-cell mass in humans. Diabetes 60:2424-2433, 2011
  •  
43.
  • Malmqvist, Olle, et al. (författare)
  • Seizures in newborn infants without hypoxic ischemic encephalopathy - antenatal and labor-related risk factors : a case-control study
  • 2020
  • Ingår i: The Journal of Maternal-Fetal & Neonatal Medicine. - : Informa Healthcare. - 1476-7058 .- 1476-4954. ; 33:5, s. 799-805
  • Tidskriftsartikel (refereegranskat)abstract
    • OBJECTIVE: To identify antepartum and intrapartum risk factors for neonatal seizures in the absence of hypoxic ischemic encephalopathy (HIE).METHODS: Population-based case-control study. Of 98 484 births, 40 newborns at 34 gestational weeks or later had seizures within the first 7 days of life. Cases (n = 40) and controls (n = 160) were retrieved from the University hospitals of Örebro for 1994-2013 and Uppsala for 2003-2013. Demographics and characteristics of pregnancy, labor, delivery, and neonatal data were analyzed. Crude odds ratio (OR) and adjusted odds ratios (AOR) with 95% confidence intervals (CIs) for antenatal and intrapartum factors were calculated using logistic regression analysis. Main outcome measure was neonatal seizures within the first 7 days of life.RESULTS: The incidence of neonatal seizures without HIE was 0.41/1000 live births. Antenatal risk factors for neonatal seizures were as follows: short maternal stature (AOR: 5.4; 1.8-16.5); previous caesarean section (AOR: 4.8; 1.5-15.0); and assisted fertilization (AOR: 6.8; 1.3-35.2). Intrapartum risk factors were as follows: induction of labor (AOR: 5.7; 1.8-17.7); preterm birth (AOR: 13.5; 3.7-48.9); and head circumference >37 cm (AOR: 6.9; 1.4-34.8).CONCLUSIONS: Preterm birth was the strongest risk factor for neonatal seizures in the absence of HIE. The results also indicate that feto-pelvic disproportion is associated with the occurrence of seizures.RATIONALE: Antepartum and intrapartum risk factors for newborn seizures in the absence of HIE were investigated in a case-control study. Out of 98 484 births at 34 gestational weeks or more, 40 newborns had seizures without HIE. All had a normal Apgar score although they later presented with seizures. Preterm birth was the strongest risk factor (OR: 13.5; 95% CI: 3.7-48.9). Our results also indicate that feto-pelvic disproportion is of importance. Furthermore, a history of prior caesarean was associated with seizures. This is the first study to assess obstetric risk factors for newborn seizures separate from those with seizures and concomitant HIE. The distinction is of importance due to different etiologies, treatments, and preventive strategies.
  •  
44.
  • Marky, Ildiko, 1940, et al. (författare)
  • Intensive chemotherapy without radiotherapy gives more than 85% event-free survival for non-Hodgkin lymphoma without central nervous involvement: a 6-year population-based study from the nordic society of pediatric hematology and oncology.
  • 2004
  • Ingår i: Journal of pediatric hematology/oncology : official journal of the American Society of Pediatric Hematology/Oncology. - : Ovid Technologies (Wolters Kluwer Health). - 1077-4114. ; 26:9, s. 555-60
  • Tidskriftsartikel (refereegranskat)abstract
    • BACKGROUND: The prognosis in childhood non-Hodgkin lymphoma (NHL) has improved dramatically during recent decades. The authors report the results from a 6-year population-based study of clinical characteristics and treatment results of NHL from the five Nordic countries. METHODS: All children younger than 15 years of age at diagnosis with NHL diagnosed from 1995 to 2000 were stratified and treated according to immunophenotypic classification and stage of disease. RESULTS: A total of 230 patients were diagnosed with primary NHL, which gives an annual incidence of 0.9/100.000 children, with a median age of 8 years. Seven percent of the children were below 3 years of age at diagnosis. The male/female ratio was 2.3 and was unrelated to age. Patients with pre-B and T-cell NHL constituted 33%, B-cell NHL 53%, and anaplastic large cell lymphoma (ALCL) 14%. According to Murphy's classification, 14% had stage 1, 17% stage 2, 50% stage 3, and 19% stage 4 disease, 12 of whom (28%) had central nervous involvement (CNS) at diagnosis. By January 1, 2003, four children had died during induction, three children died in remission (2, 6, and 26 months from diagnosis), and 24 children experienced a relapse. At 5 years, the probability of event-free survival (p-EFS) was 86+/-2% for all children. The 5-year p-EFS values for stages 1 through 4 were 94%, 97%, 83%, and 79%, respectively. The 5-year p-EFS values were 91% for B-cell, 87% for pre-B, 81% for ALCL, and 79% for T-cell NHL. The 12 patients with CNS involvement at diagnosis had a significantly poorer outcome than stage 4 patients with CNS involvement (p-EFS = 50% vs. 90%, P < 0.01). The 218 patients without CNS disease at diagnosis had a 5-year p-EFS of 88%. CONCLUSIONS: With modern intensive chemotherapy, more than 85% of NHL patients will achieve long-lasting first remission. In the future, preventing death during induction and remission and improving therapy for patients with CNS disease would have a major impact on the overall p-EFS.
  •  
45.
  • Marsell, Richard, et al. (författare)
  • GSK-3 inhibition by an orally active small molecule increases bone mass in rats
  • 2012
  • Ingår i: Bone. - : Elsevier BV. - 8756-3282 .- 1873-2763. ; 50:3, s. 619-627
  • Tidskriftsartikel (refereegranskat)abstract
    • Glycogen synthase kinase 3β (GSK-3β) actions are central in the canonical Wnt pathway, important in many biological processes and a potential drug target for treating several diseases. It is appreciated that a balanced Wnt canonical signaling is crucial for the maintenance of normal bone mass. In this study we investigated the effects of a potent orally active GSK-3 inhibitor, AZD2858, on bone mass in rats. Treatment (1μM) of human osteoblast cells with AZD2858 in vitro increased β-catenin levels after a short period of time. In rats, oral AZD2858 treatment caused a dose-dependent increase in trabecular bone mass compared to control after a two-week treatment with a maximum effect at a dose of 20mg/kg once daily (total BMC: 172% of control; p<0.001). A small but significant effect was also seen at cortical sites (total BMC: 111% of control; p<0.001). Biomechanical testing demonstrated an increase in both vertebral compression strength at a dose of 20mg/kg once daily (Load at failure: 370% of control, p<0.001) and diaphyseal strength of femora subjected to a three point bending test (Load at failure: 115% of control; p<0.01). Furthermore, histomorphometry showed a dramatic increase in bone formation indices, and serum markers of both bone formation (Osteocalcin, 146% of control; p<0.001) and resorption (CTX, 189% of control; p<0.001) were elevated. Our conclusion is that a GSK-3 inhibitor drug may prove effective as an anabolic strategy in the treatment of diseases characterized by low bone mass, since AZD2858 has extensive bone building effects at predominantly trabecular sites.
  •  
46.
  • Mermod, Philippe, et al. (författare)
  • 95 MeV neutron scattering on hydrogen, deuterium, carbon and oxygen
  • 2006
  • Ingår i: Physical Review C. Nuclear Physics. - 0556-2813 .- 1089-490X. ; 74:5, s. 054002-
  • Tidskriftsartikel (refereegranskat)abstract
    • Three neutron-deuteron scattering experiments at 95 MeV have been performed recently at The Svedberg Laboratory in Uppsala. Subsets of the results of these experiments have been reported in two short articles, showing clear evidence for three-nucleon force effects. In this paper, we present a more detailed description of the experimental methods as well as further discussion of the results. In addition to neutron-deuteron scattering data, neutron-proton and C12(n,n) elastic scattering data have been measured for normalization purposes, and O16(n,n) data have been obtained for the first time at this energy. It was possible to extract C12(n,n') and O16(n,n') inelastic scattering cross sections to excited states below 12 MeV excitation energy. The inelastic scattering data (for both carbon and oxygen) are shown to have a significant impact on the determination of nuclear recoil kerma coefficients.
  •  
47.
  •  
48.
  • Nordin, Ulrika, et al. (författare)
  • Övervakning av ringlav i Gävleborgs län 1996 - 2013
  • 2016
  • Rapport (övrigt vetenskapligt/konstnärligt)abstract
    • Sedan år 1996 pågår en övervakning av ringlav Evernia divaricata på 19 lokaler i Gävleborgs län. I denna rapport redovisas resultatet från den senaste återinventeringen år 2012/2013, samt jämförelser med tidigare inventeringar. Övervakningen omfattar dels räkning av lavbålar i en provyta på varje lokal, dels räkning av antalet träd med ringlav i transekter som går ut 50 meter från provytan i alla fyra väderstreck (”grovkartering”).Resultatet från inventeringen i provytan visar att antalet ringlavbålar har minskat på 12 ringlavslokaler och ökat på 7 lokaler sedan inventeringsstarten. Antalet träd med ringlav visar liknande trend, en minskning på 13 lokaler och en ökning på 5 lokaler. Men den senaste trenden, sedan år 2002, visar att minskningar av antalet ringlavbålar skett på alla 19 lokalerna. För antalet ringlavsträd under motsvarande period har minskningar skett på 17 lokaler, ökning på en lokal och oförändrat antal på en lokal.I grovkarteringen har antal träd med ringlav, under perioden år 1999 till år 2012/2013, minskat på 13 lokaler, ökat på 5 lokaler och är oförändrat på en lokal.Både storskaliga och lokala faktorer som kan ha påverkat ringlaven diskuteras i rapporten. Till de storskaliga hör förtätning av skogsbestånden; klimatvariationer och stormen Dagmar. Mer lokala påverkansfaktorer som diskuteras är luftföroreningar, markavvattning, skogsbruk (hygge) och utdöendeskuld.Slutsatsen från övervakningen är att ringlavens framtid fortfarande är osäker i länet. Även på två lokaler som är klassade som nyckelbiotoper har avverkningar skett. På en lokal har en kraftig minskning skett på grund av dikning och vägbygge som har skett ganska långt tillbaka i tiden. På ytterligare några lokaler har ringlaven minskat av okänd anledning, eventuellt som en följd av utdöendeskuld. På de fyra sydligaste lokalerna är utvecklingen alarmerande. Södra Gästrikland har tidigare ansetts vara ett starkt fäste för ringlaven, men nu verkar det som om den helt håller på att försvinna från området.
  •  
49.
  • Olle, Jonsson B., et al. (författare)
  • Allozyme diversity and geographic variation in the widespread coastal sedge, Carex arenaria
  • 2000
  • Ingår i: Diversity & distributions. - : Wiley. - 1366-9516 .- 1472-4642. ; 6:2, s. 65-80
  • Tidskriftsartikel (refereegranskat)abstract
    • Allozyme electrophoresis was used to investigate the structure of genetic variation in the rhizomatous coastal sedge, Carex arenaria, throughout its European range - from the SW Iberian peninsula to the Baltic region. Material was sampled from 77 sites in five geographic regions. Nine of the 13 investigated loci were polymorphic in the total material and there were interregional differences in the number of polymorphic loci per site and the percentage of variable sites. In the Scandinavia/Baltic region only 61% of the sites contained at least one locus with more than one allele, whereas all the British and SW Iberian sites were variable. There was a general tendency for the regional frequencies of the less common alleles at individual loci to decline from SW to NE. The mean (over loci and sites) within-site gene diversity (H(site)) was 0.064 (in calculations based on the number of observed multilocus allozyme genotypes within each sampling site). Although there was considerable variation between geographically adjacent sites, within-site diversity showed a general decrease from SW to NE in Europe. There were significant differences in within-region gene diversity (H(reg)) for the four most variable loci between the five regions. H(reg) generally decreased from SW to NE Europe and most loci showed the highest diversity in the SW Iberian peninsula and the Bay of Biscay regions. The mean (over loci) gene diversity in the total material (H(tot)) was 0.070 and the levels of diversity in Carex arenaria are substantially lower than is usual in rhizomatous sedges. The within-site, between-site and between-regional components of the total diversity were 92.4%, 2.5% and 5.1%, respectively. The low levels of overall gene diversity in C. arenaria and the successive decrease in diversity from SW to NE are interpreted in terms of the species' history of post-glacial spread into northern Europe. Despite the overall northwards decrease in diversity, the widespread occurrence of less common alleles and the lack of regional deviations from Hardy-Weinberg genotype frequency expectations suggest that C. arenaria is not predominantly self-fertilized.
  •  
50.
  • Prentice, Honor C., et al. (författare)
  • Fragmented grasslands on the Baltic island of Öland : Plant community composition and land-use history
  • 2007
  • Ingår i: Acta Phytogeographica Suecica. - 0084-5914. ; 88, s. 83-94
  • Forskningsöversikt (refereegranskat)abstract
    • The relationships between properties of present landscapes and species diversity within fragmented grasslands have been the subject of many studies. However the potential roles of grassland history and past landscape structure as determinants of diversity have not been widely studied. We therefore focus on these roles with an overview of patterns of variation in plant community composition in grassland fragments, of known ages, within a 22 km2 site around the village of Jordtorp on the Baltic island of Öland. While the frequencies of individual vascular plant species in 328 50 cm × 50 cm quadrats showed trends related to grassland continuity and previous land use, the dominant gradients of plant community composition were interpreted in terms of gradients of soil moisture and eutrophication. Because relationships between grassland age and plant community composition are confounded by local grassland eutrophication, it is difficult to use the present grassland data set to draw conclusions about the extent to which the distributions of individual species reflect a long history of continuous grassland management or an absence of eutrophication. Our results suggest that studies that attempt to explore associations between grassland age and fine-scale species richness, or the occurrence of individual species, should be based on sampling rules that standardize the type of plant community that is to be compared between grassland fragments.
  •  
Skapa referenser, mejla, bekava och länka
  • Resultat 1-50 av 67
Typ av publikation
tidskriftsartikel (48)
rapport (8)
doktorsavhandling (6)
konstnärligt arbete (1)
bok (1)
annan publikation (1)
visa fler...
konferensbidrag (1)
forskningsöversikt (1)
licentiatavhandling (1)
visa färre...
Typ av innehåll
refereegranskat (50)
övrigt vetenskapligt/konstnärligt (17)
Författare/redaktör
Melander, Olle (11)
Nilsson, Peter (8)
Lyssenko, Valeriya (8)
Groop, Leif (8)
Korsgren, Olle (4)
Kämpe, Olle (4)
visa fler...
Lindholm, Eero (4)
Kristiansson, Erik, ... (3)
Tuomi, Tiinamaija (3)
Ahlqvist, Emma (3)
Johansson, Cecilia (3)
Larsson, Sune (3)
Lind, Lars (3)
Ohlsson, Claes, 1965 (3)
Nilsson, Leif (3)
Chen, H. (2)
Meyer, J. (2)
James, A. (2)
Peters, A (2)
Kumar, A. (2)
Mahajan, A. (2)
Salehi, S Albert (2)
Shah, S (2)
Balkau, B. (2)
Langenberg, C. (2)
Raychaudhuri, S (2)
Kong, A. (2)
Stenlund, Hans (2)
Fontanillas, P (2)
Pedersen, NL (2)
Loos, RJF (2)
Jonsson, Håkan (2)
Isomaa, B. (2)
Tuomi, T. (2)
Fadista, Joao (2)
Hansson, Ola (2)
Roden, M (2)
Johnsson, Martin (2)
Watanabe, Y. (2)
Hofman, A (2)
Muhleisen, TW (2)
Nothen, MM (2)
Uitterlinden, AG (2)
Nilsson, Lena (2)
Syvänen, Ann-Christi ... (2)
Bentzer, Peter (2)
Gertow, K (2)
Li, M. (2)
Jensen, Per, 1956- (2)
Prentice, Honor C (2)
visa färre...
Lärosäte
Uppsala universitet (27)
Lunds universitet (18)
Göteborgs universitet (14)
Linköpings universitet (9)
Karolinska Institutet (8)
Naturvårdsverket (5)
visa fler...
Chalmers tekniska högskola (5)
Umeå universitet (4)
Kungliga Tekniska Högskolan (4)
Stockholms universitet (3)
Linnéuniversitetet (3)
Örebro universitet (2)
Malmö universitet (2)
Högskolan Kristianstad (1)
RISE (1)
Karlstads universitet (1)
Kungl. Konsthögskolan (1)
visa färre...
Språk
Engelska (58)
Svenska (9)
Forskningsämne (UKÄ/SCB)
Medicin och hälsovetenskap (26)
Naturvetenskap (22)
Lantbruksvetenskap (2)
Teknik (1)
Samhällsvetenskap (1)
Humaniora (1)

År

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy