| 1. |
|
|
| 2. |
|
|
| 3. |
- Palmer, Nathan, et al.
(författare)
-
Identification PMS1 and PMS2 as potential meiotic substrates of CDK2 activity
- 2023
-
Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 18:3
-
Tidskriftsartikel (refereegranskat)abstract
- Cyclin dependent-kinase 2 (CDK2) plays important functions during the mitotic cell cycle and also facilitates several key events during germ cell development. The majority of CDK2's known meiotic functions occur during prophase of the first meiotic division. Here, CDK2 is involved in the regulation of meiotic transcription, the pairing of homologous chromosomes, and the maturation of meiotic crossover sites. Despite that some of the CDK2 substrates are known, few of them display functions in meiosis. Here, we investigate potential meiotic CDK2 substrates using in silico and in vitro approaches. We find that CDK2 phosphorylates PMS2 at Thr337, PMS1 at Thr331, and MLH1 in vitro. Phosphorylation of PMS2 affects its interaction with MLH1 to some degree. In testis extracts from mice lacking Cdk2, there are changes in expression of PMS2, MSH2, and HEI10, which may be reflective of the loss of CDK2 phosphorylation. Our work has uncovered a few CDK2 substrates with meiotic functions, which will have to be verified in vivo. A better understanding of the CDK2 substrates will help us to gain deeper insight into the functions of this universal kinase.
|
|
| 4. |
- Patel, Vinay, et al.
(författare)
-
Development of vascular cambium in the leaf rachis of Kigelia Africana (Lam.) Benth
- 2010
-
Ingår i: PRAJÑĀ - Journal of Pure and Applied Sciences. - Gujarat, India : Sardar Patel University. - 0975-2595. ; 18:1-4, s. 40-43
-
Tidskriftsartikel (refereegranskat)abstract
- The structural changes during the vascular cambium development from procambium in the leaf rachis of Kigelia africana bearing leaflets of different developmental stages (young, mature and old rachis) were investigated. The different regions from the internodes of rachis bearing young, copper brown coloured leaves revealed the sequential stages of cambial development. Terminal region of the rachis showed developing procambium along with protoxylem and protopholem derivatives, middle regions exhibited the development of interfascicular cambium whereas basal region showed complete ring of vascular cambium with secondary phloem derivatives. Rachis bearing brownish coloured leaflets showed an active cambium with both secondary xylem and phloem derivatives indicating bidirectional cambial growth. Rachis with fully matured dark green leaflets was characterized by the presence of dormant cambium surrounded by mature xylem and phloem elements and occurrence of calcium oxalate crystals in the phloem parenchyma cells. The study also describes the pattern of vascular cambial development in the leaf rachis of Kigelia, a deciduous tree and it was compared with development of vascular cambium in the leaves of conifers and evergreen dicotyledons. Influence of leaf maturity on cambial activity and secondary xylem development is discussed.
|
|
