| 1. |
- Gkoutzamanis, V. G., et al.
(författare)
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Thermal Management System Considerations for a Hybrid-Electric Commuter Aircraft
- 2022
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Ingår i: Journal of thermophysics and heat transfer. - : AIAA International. - 0887-8722 .- 1533-6808. ; 36:3, s. 650-666
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Tidskriftsartikel (refereegranskat)abstract
- When it comes to novel aircraft concepts, thermal management system (TMS) design is a ubiquitous task, even at the conceptual design phase. This is owing to its impact on the total weight of the aircraft, cooling drag, and overall performance. The commuter air transportation has recently regained attention and is seen as a solution to employ partial or full electrification in the upcoming decades due to its low power requirement and potential benefit of faster “door-to-door” traveling. This work examines the TMS characteristics to cool a battery-powered aft-fan engine. A literature review is initially performed on other research associated with TMS design. The development and weight evaluation of the baseline TMS for this type of propulsive technology are then presented, including the characterization of system redundancy effects on the overall TMS weight. Results show that the TMS design is a function of the selected propulsive configuration and energy management throughout the mission. Primarily, this relates to the cooling method selected, the heat exchangers as the major mass contributors of the TMS, the positioning of components used for the propulsive configuration, and the imposed certification constraints. Finally, the selected TMS design is calculated to have a combined specific cooling of 0.79 kW∕kg.
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| 2. |
- Volgenant, C. M. C., et al.
(författare)
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Low prevalence of multi-resistant bacteria in undergraduate dental students; an observational case-control multi-centre study in Europe
- 2021
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Ingår i: Journal of Oral Microbiology. - : Informa UK Limited. - 2000-2297. ; 13:1
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Tidskriftsartikel (refereegranskat)abstract
- Objective: This study assessed the prevalence of MRSA, ESBL and VRE in students from four dental schools in Europe. Methods: The hand, tongue and nostrils of the students who treated patients (study group) and who did not treat patients (control group) were sampled. After incubation in TSB and subculturing in the presence of 4 mu g/ml oxacillin, positive cultures were identified for Staphylococcus aureus by Mannitol salt agar and agglutination tests. The presence of MRSA was confirmed by specific PCR on the species and on the SSCmec genes. ESBL and VRE were isolated using specific CHROMagar and confirmed using antibiotic sensitivity tests. Results: Of the 879 students who participated in this study (454 students which treated patients, 425 controls) a total of 50 students (5.7%) tested positive for a multi-drug resistant bacterium (MDRB); 13 (1.5%) students tested positive for MRSA, 26 (3.0%) for ESBL and 12 (1.4%) for VRE. No statistically significant differences were found between the students who treated patients compared to the control group for any of the MDRB and study centres, excluding MRSA carriage in the Italian student population. The use of antibiotics the year before sampling, was positively associated with the presence of an MDRB (OR 2.0; 95% Confidence Interval 1.10-3.68; p = 0.02). Conclusion: The risk for MDRB carriage and sequential transmission of MDRB for dental health care students and their patients were acceptably low.
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| 3. |
- Belibasakis, G N, et al.
(författare)
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Cytokine responses of human gingival fibroblasts to Actinobacillus actinomycetemcomitans cytolethal distending toxin.
- 2005
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Ingår i: Cytokine. - : Elsevier BV. - 1043-4666 .- 1096-0023. ; 30:2, s. 56-63
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Tidskriftsartikel (refereegranskat)abstract
- Actinobacillus actinomycetemcomitans is implicated in the pathogenesis of localized aggressive periodontitis, and has the capacity to express a cytolethal distending toxin (Cdt). Gingival fibroblasts (GF) are resident cells of the periodontium, which can express several osteolytic cytokines. The aims of this study were a) to investigate the role of Cdt in A. actinomycetemcomitans-induced expression of osteolytic cytokines and their cognate receptors in GF and b) to determine if the previously demonstrated induction of receptor activator of NFkappaB ligand (RANKL) by A. actinomycetemcomitans is mediated by these pro-inflammatory cytokines or by prostaglandin E(2) (PGE(2)). A. actinomycetemcomitans clearly induced interleukin (IL)-6, IL-1beta, and to a minimal extent, tumor necrosis factor (TNF)-alpha mRNA expression. At the protein level, IL-6 but not IL-1beta or TNF-alpha expression was stimulated. The mRNA expression of the different receptor subtypes recognizing IL-6, IL-1beta and TNF-alpha was not affected. A cdt-knockout strain of A. actinomycetemcomitans had similar effects on cytokine and cytokine receptor mRNA expression, compared to its parental wild-type strain. Purified Cdt stimulated IL-6, but not IL-1beta or TNF-alpha protein biosynthesis. Antibodies neutralizing IL-6, IL-1 or TNF-alpha, and the PGE(2) synthesis inhibitor indomethacin, did not affect A. actinomycetemcomitans-induced RANKL expression. In conclusion, a) A. actinomycetemcomitans induces IL-6 production in GF by a mechanism largely independent of its Cdt and b) A. actinomycetemcomitans-induced RANKL expression in GF occurs independently of IL-1, IL-6, TNF-alpha, or PGE(2).
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| 4. |
- Belibasakis, G N, et al.
(författare)
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The cytolethal distending toxin induces receptor activator of NF-kappaB ligand expression in human gingival fibroblasts and periodontal ligament cells.
- 2005
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Ingår i: Infection and immunity. - 0019-9567 .- 1098-5522. ; 73:1, s. 342-51
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Tidskriftsartikel (refereegranskat)abstract
- Actinobacillus actinomycetemcomitans is associated with localized aggressive periodontitis, a disease characterized by rapid loss of the alveolar bone surrounding the teeth. Receptor activator of NF-kappaB Ligand (RANKL) and osteoprotegerin (OPG) are two molecules that regulate osteoclast formation and bone resorption. RANKL induces osteoclast differentiation and activation, whereas OPG blocks this process by acting as a decoy receptor for RANKL. The purpose of this study was to investigate the effect of A. actinomycetemcomitans on the expression of RANKL and OPG in human gingival fibroblasts and periodontal ligament cells. RANKL mRNA expression was induced in both cell types challenged by A. actinomycetemcomitans extract, whereas OPG mRNA expression remained unaffected. Cell surface RANKL protein was also induced by A. actinomycetemcomitans, whereas there was no change in OPG protein secretion. A cytolethal distending toxin (Cdt) gene-knockout strain of A. actinomycetemcomitans did not induce RANKL expression, in contrast to its wild-type strain. Purified Cdt from Haemophilus ducreyi alone, or in combination with extract from the A. actinomycetemcomitans cdt mutant strain, induced RANKL expression. Pretreatment of A. actinomycetemcomitans wild-type extract with Cdt antiserum abolished RANKL expression. In conclusion, A. actinomycetemcomitans induces RANKL expression in periodontal connective tissue cells. Cdt is crucial for this induction and may therefore be involved in the pathological bone resorption during the process of localized aggressive periodontitis.
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| 5. |
- Collins, M D, et al.
(författare)
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Characterization of Actinomyces isolates from infected root canals of teeth: description of Actinomyces radicidentis sp. nov.
- 2000
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Ingår i: Journal of clinical microbiology. - 0095-1137. ; 38:9, s. 3399-403
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Tidskriftsartikel (refereegranskat)abstract
- Two strains of a previously undescribed Actinomyces-like bacterium were recovered in pure culture from infected root canals of teeth. Analysis by biochemical testing and polyacrylamide gel electrophoresis of whole-cell proteins indicated that the strains closely resembled each other phenotypically but were distinct from previously described Actinomyces and Arcanobacterium species. Comparative 16S rRNA gene-sequencing studies showed the bacterium to be a hitherto unknown subline within a group of Actinomyces species which includes Actinomyces bovis, the type species of the genus. Based on phylogenetic and phenotypic evidence, we propose that the unknown bacterium isolated from human clinical specimens be classified as Actinomyces radicidentis sp. nov. The type strain of Actinomyces radicidentis is CCUG 36733.
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| 6. |
- Hahn Berg, IC, et al.
(författare)
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Proteolytic degradation of oral biofilms in vitro and in vivo: potential of proteases originating from Euphausia superba for plaque control
- 2001
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Ingår i: European Journal of Oral Sciences. - 0909-8836 .- 1600-0722. ; 109, s. 316-324
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Tidskriftsartikel (refereegranskat)abstract
- This paper deals with enzymatic removal of dental plaque, in vitro as well as in vivo, using proteases from the Antarctic krill shrimp (Euphausia superba), referred to as Krillase®. Krillase exhibits both endo- and exopeptidase activity but has no microbicidal effect. In model systems with pure cultures of oral microorganisms, Krillase demonstrated inhibition of microbial adhesion to saliva-coated hydroxyapatite. Furthermore, a protocol for the growth of reproducible in vitro plaque films has been developed, and effects of Krillase on the plaque film were investigated by means of scanning electron microscopy (SEM). The results showed that Krillase efficiently released microorganisms from plaque in vitro, the effect being dependent on the enzymatic activity. The surface energy of the substratum had a minor influence on the formation and removal of plaque in vitro. Ellipsometric studies on the formation and enzymatic removal of a salivary pellicle indicated that the enzymatic effect on plaque may partly depend on degradation of the salivary pellicle. Krillase was also able to remove plaque accumulated on dentures in vivo. Our results demonstrate the potential of Krillase for plaque control, and that these enzymes are worthy of further investigations including clinical studies and work to find a suitable vehicle
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| 7. |
- HILDINGSSON, L, et al.
(författare)
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HIGH-SPIN PHENOMENA IN OS-174
- 1992
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Ingår i: Nuclear Physics A. - 0375-9474 .- 1873-1554. ; 545:4, s. 871-888
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Tidskriftsartikel (refereegranskat)abstract
- High-spin states of 174Os have been investigated by means of the (Nd(S, 4n)Os)-Nd-146-S-32-Os-174 reaction using the ESSA30 multidetector system. The decay is dominated by the ground-state positive-parity band, two negative-parity 4- and 5- bands and another band starting at spin 9. Deformed shell-model calculations have been carried out to interpret the observed band structures. The role of the strongly shape-driving, non-aligned, pi-h9/2 configuration in the low-spin region of the ground-state band is discussed. The first band crossing is interpreted as due to the nu-i13/2 alignment. The two lowest side-bands are understood in terms of coupling to octupole excitations.
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| 8. |
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| 9. |
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| 10. |
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| 11. |
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| 12. |
- Johansson, Anders, et al.
(författare)
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Serum-mediated release of leukotoxin from the cell surface of the periodontal pathogen Actinobacillus actinomycetemcomitans.
- 2003
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Ingår i: European Journal of Oral Sciences. - : Wiley. - 0909-8836 .- 1600-0722. ; 111:3, s. 209-215
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Tidskriftsartikel (refereegranskat)abstract
- The leukotoxin of the periodontopathogen Actinobacillus actinomycetemcomitans is an important virulence factor that lyses human neutrophils and monocytes and thus, it may enable the bacterium to evade the local host defense. The toxin also induces degranulation of neutrophils and cytokine release in monocytes. To trigger these biological activities, leukotoxin has to be released from the bacterium and diffuse into the periodontal tissues. To date, the conditions found to cause toxin release have been artificial and have included high ion concentration and alkaline conditions. To study the release of the toxin under conditions mimicking the natural environment of the periodontium the ability of human serum to enable leukotoxin release from the bacterial surface was examined. Suspensions of leukotoxic A. actinomycetemcomitans strains were incubated with various concentrations of human serum or serum albumin. The suspensions were centrifuged and the leukotoxin in the supernatants or the cell pellets was detected by gel electrophoresis and immunoblotting. Serum was found to cause the rapid release of leukotoxin from the bacteria in a concentration-dependent manner. Pure albumin exhibited a similar effect. The leukotoxin released was active against human neutrophils. Only a minor proportion of it was associated with membranous vesicles produced by the bacteria. The results indicate that serum, a fluid closely related to the exudate in inflamed periodontal pockets, releases leukotoxin from the cell surface of A. actinomycetemcomitans. The process may enable the diffusion of the toxin from the bacterial biofilm into the surrounding tissues, where it can exert its biological effect.
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| 13. |
- Kelk, Peyman, 1976-, et al.
(författare)
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Abundant secretion of bioactive interleukin-1beta by human macrophages induced by Actinobacillus actinomycetemcomitans leukotoxin.
- 2005
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Ingår i: Infection and immunity. - 0019-9567 .- 1098-5522. ; 73:1, s. 453-8
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Tidskriftsartikel (refereegranskat)abstract
- Actinobacillus actinomycetemcomitans produces a leukotoxin that selectively kills human leukocytes. Recently, we reported that macrophages are highly sensitive to leukotoxin and that their lysis involves activation of caspase 1. In this study, we show that leukotoxin also induces the production and release of proinflammatory cytokines from human macrophages. The macrophages were challenged with leukotoxin or lipopolysaccharide (LPS) from A. actinomycetemcomitans or LPS from Escherichia coli, and the production and secretion of interleukin-1beta (IL-1beta), IL-6, and tumor necrosis factor alpha (TNF-alpha) were determined at the mRNA and protein levels by reverse transcription-PCR and enzyme-linked immunosorbent assay, respectively. Leukotoxin (1 to 30 ng/ml) induced abundant production and secretion of IL-1beta, while the effects on IL-6 and TNF-alpha production were limited. Leukotoxin (1 ng/ml) caused a 10-times-higher release of IL-1beta than did LPS (100 ng/ml). The secreted IL-1beta was mainly the bioactive 17-kDa protein. At higher concentrations (>30 ng/ml), leukotoxin caused secretion of mainly inactive cytokine, the 31-kDa pro-IL-1beta. The presence of specific antibodies to IL-1beta or of a caspase 1 inhibitor blocked the secretion and production of the cytokine. Supernatants of leukotoxin-challenged macrophages stimulated bone resorption when tested in a mouse calvarial model. The activity could be blocked by an IL-1 receptor antagonist or specific antibodies to IL-1beta. We concluded that A. actinomycetemcomitans leukotoxin can trigger abundant production and secretion of bioactive IL-1beta by human macrophages, which is mediated by activation of caspase 1.
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| 14. |
- Kelk, Peyman, 1976-, et al.
(författare)
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Caspase 1 involvement in human monocyte lysis induced by Actinobacillus actinomycetemcomitans leukotoxin
- 2003
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Ingår i: Infection and Immunity. - 0019-9567 .- 1098-5522. ; 71:8, s. 4448-4455
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Tidskriftsartikel (refereegranskat)abstract
- Actinobacillus actinomycetemcomitans, an oral bacterium implicated in the etiology of periodontal diseases, produces a leukotoxin that selectively lyses primate neutrophils and monocytes, the major populations of defense cells in the periodontium. Though lysis requires expression of the receptor lymphocyte function-associated molecule 1 (LFA-1) on the cell surface, not all LFA-1-expressing leukocyte populations are equally susceptible to the toxin. In this study, the susceptibility of human leukocytes to leukotoxin-induced lysis is compared to their expression of LFA-1 and the activity of caspase 1. Cytolysis was determined by the activity of lactate dehydrogenase released from peripheral human leukocytes after 1-h exposure to leukotoxin. Monocytes were lysed at leukotoxin concentrations of > or = 5 ng/ml, while the corresponding values for neutrophils and lymphocytes were approximately 10 times greater. Similar LFA-1 expression was found in all susceptible cell populations irrespective of their degree of sensitivity to the toxin. Exposure of monocytes to leukotoxin increased their caspase 1 activity about fivefold within 10 to 20 min. Presence of the caspase 1 inhibitor Ac-YVAD-CMK significantly blocked the leukotoxin-induced lysis of monocytes only. At sublytic concentrations, leukotoxin induced no apoptotic activity in monocytes, as revealed by the lack of caspase 3 activation and DNA fragmentation. Monocytes are the most lysis-sensitive leukocytes for A. actinomycetemcomitans leukotoxin. Their lysis by this toxin depends on caspase 1 activation and proceeds through a process that differs from classical apoptosis.
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| 15. |
- Kyprianidis, Konstantinos G., et al.
(författare)
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EVA : A Tool for EnVironmental Assessment of Novel Propulsion Cycles
- 2008
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Ingår i: <em><em></em></em>ASME Turbo Expo 2008: Power for Land, Sea, and AirVolume 2: Controls, Diagnostics and Instrumentation; Cycle Innovations; Electric PowerBerlin, Germany, June 9–13, 2008. - 9780791843123 - 0791838242 ; , s. 547-556
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Konferensbidrag (refereegranskat)abstract
- This paper presents the development of a tool for EnVironmental Assessment (EVA) of novel propulsion cycles implementing the Technoeconomical Environmental and Risk Analysis (TERA) approach. For nearly 3 decades emissions certification and legislation has been mainly focused on the landing and take-off cycle. Exhaust emissions measurements of NOx, CO and unburned hydrocarbons are taken at Sea Level Static (SLS) conditions for 4 different power settings (idle, descent, approach and take-off) and are consecutively used for calculating the total emissions during the ICAO landing and take-off cycle. With the global warming issue becoming ever more important, stringent emissions legislation is soon to follow, focusing on all flight phases of an aircraft. Unfortunately, emissions measurements at altitude are either extremely expensive, as in the case of altitude test facility measurements, or unrealistic, as in the case of direct in flight measurements. Compensating for these difficulties, various existing methods can be used to estimate emissions at altitude from ground measurements. Such methods, however, are of limited help when it comes to assessing novel propulsion cycles or existing engine configurations with no SLS measurements available. The authors are proposing a simple and fast method for the calculation of SLS emissions, mainly implementing ICAO exhaust emissions data, corrections for combustor inlet conditions and technology factors. With the SLS emissions estimated, existing methods may be implemented to calculate emissions at altitude. The tool developed couples emissions predictions and environmental models together with engine and aircraft performance models in order to estimate the total emissions and Global Warming Potential of novel engine designs during all flight phases (i.e. the whole flight cycle). The engine performance module stands in the center of all information exchange. In this study, EVA and the described emissions prediction methodology have been used for the preliminary design analysis of three spool high bypass ratio turbofan engines. The capability of EVA to radically explore the design space available in novel engine configurations, while accounting for fuel burn and global warming potential during the whole flight cycle of an aircraft, is illustrated.
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| 16. |
- Kyprianidis, Konstantinos, et al.
(författare)
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Uncertainty in gas turbine thermo-fluid modelling and its impact on performance calculations and emissions predictions at aircraft system level
- 2012
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Ingår i: Proceedings of the Institution of Mechanical Engineers, Part G: Journal of Aerospace Engineering. - : SAGE Publications. - 0954-4100 .- 2041-3025. ; 226:2, s. 163-181
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Tidskriftsartikel (refereegranskat)abstract
- In this article, various aspects of thermo-fluid modelling for gas turbines are described and the impact on performance calculations and emissions predictions at aircraft system level is assessed. Accurate and reliable fluid modelling is essential for any gas turbine performance simulation software as it provides a robust foundation for building advanced multi-disciplinary modelling capabilities. Caloric properties for generic and semi-generic gas turbine performance simulation codes can be calculated at various levels of fidelity; selection of the fidelity level is dependent upon the objectives of the simulation and execution time constraints. However, rigorous fluid modelling may not necessarily improve performance simulation accuracy unless all modelling assumptions and sources of uncertainty are aligned to the same level.A comprehensive analysis of thermo-fluid modelling for gas turbines is presented, and the fluid models developed are discussed in detail. Common technical models, used for calculating caloric properties, are compared while typical assumptions made in fluid modelling, and the uncertainties induced, are examined. Several analyses, which demonstrate the effects of composition, temperature, and pressure on caloric properties of working media for gas turbines, are presented. The working media examined include dry air and combustion products for various fuels and H/C ratios. The uncertainty induced in calculations by (a) using common technical models for evaluating fluid caloric properties and (b) ignoring dissociation effects is examined at three different levels: (i) component level, (ii) engine level, and (iii) aircraft system level. An attempt is made to shed light on the trade-off between improving the accuracy of a fluid model and the accuracy of a multi-disciplinary simulation at aircraft system level, against computational time penalties. The validity of the ideal gas assumption for future turbofan engines and novel propulsion cycles is discussed. The results obtained demonstrate that accurate modelling of the working fluid is essential, especially for assessing novel and/or aggressive cycles at aircraft system level. Where radical design space exploration is concerned, improving the accuracy of the fluid model will need to be carefully balanced with the computational time penalties involved.
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| 17. |
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| 18. |
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