| 1. |
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Agglomeration, clusters and entrepreneurship : Studies in regional economic development
- 2014
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Samlingsverk (redaktörskap) (övrigt vetenskapligt/konstnärligt)abstract
- Regional economic development has experienced considerable dynamism over recent years. Perhaps the most notable cases were the rise of China and India to emergent country status by the turn of the millennium. With time now for hindsight, this book identifies some of the key forces behind these development successes, namely agglomeration, clusters and entrepreneurship.
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| 2. |
- Allamani, Allaman, et al.
(författare)
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Contextual Determinants of Alcohol Consumption Changes and Preventive Alcohol Policies : A 12-Country European Study in Progress 2011
- 2011
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Ingår i: Substance Use & Misuse. - : Informa UK Limited. - 1082-6084 .- 1532-2491. ; 46:10, s. 1288-1303
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Tidskriftsartikel (refereegranskat)abstract
- Beginning with France in the 1950s, alcohol consumption has decreased in Southern European countries with few or no preventive alcohol policy measures being implemented, while alcohol consumption has been increasing in Northern European countries where historically more restrictive alcohol control policies were in place, even though more recently they were loosened. At the same time, Central and Eastern Europe have shown an intermediate behavior. We propose that country-specific changes in alcohol consumption between 1960 and 2008 are explained by a combination of a number of factors: (1) preventive alcohol policies and (2) social, cultural, economic, and demographic determinants. This article describes the methodology of a research study designed to understand the complex interactions that have occurred throughout Europe over the past five decades. These include changes in alcohol consumption, drinking patterns and alcohol-related harm, and the actual determinants of such changes
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| 3. |
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| 4. |
- Andersson, Börje, et al.
(författare)
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Thermal stresses in large butt-welded plates
- 1981
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Ingår i: Journal of thermal stresses. - : Informa UK Limited. - 0149-5739 .- 1521-074X. ; 4:3-4, s. 491-500
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Tidskriftsartikel (refereegranskat)abstract
- In shipyard panel-production lines several rectangular plates ae butt welded to form large panels. One problem is the tendency of hot cracking in the end portion of the butt weld. In this paper transient temperature fields and stress fields in butt-welded plates are calculated using the finite-element method. The calculations are performed for different welding speeds, for different heat inputs, and for one-sided and two-sided welding.
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| 5. |
- Andersson, Håkan, 1944, et al.
(författare)
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Astatine-211-labeled antibodies for treatment of disseminated ovarian cancer: an overview of results in an ovarian tumor model
- 2003
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Ingår i: Clin Cancer Res. - 1078-0432. ; 9:10 Pt 2
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Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: The aim of the study was to establish and refine a preclinical model to alpha-immunoradiotherapy of ovarian cancer. EXPERIMENTAL DESIGN: At-211 was produced by cyclotron irradiation of a bismuth-209 target and isolated using a novel dry distillation procedure. Monoclonal antibodies were radiohalogenated with the intermediate reagent N-succinimidyl 3-(trimethylstannyl)benzoate and characterized in terms of radiochemical yield and in vitro binding properties. In vitro OVCAR-3 cells were irradiated using an external Cobalt-60 beam, as reference, or At-211-albumin and labeled antibody. Growth assays were used to establish cell survival. A Monte Carlo program was developed to simulate the energy imparted and the track length distribution. Nude mice were used for studies of WBC depression, with various activities of Tc-99m antibodies, as reference, and At-211 antibodies. In efficacy studies, OVCAR-3 cells were inoculated i.p., and animals were treated 2 weeks later. The animals were either dissected 6 weeks later or followed-up for long-term survival. RESULTS: A rapid distillation procedure, as well as a rapid and high-yield, single-pot labeling procedure, was achieved. From growth inhibition data, the relative biological effectiveness of the alpha-emission for OVCAR-3 cells was estimated to be approximately 5, which is in the same range as found in vivo for hematological toxicity. At-211 MOv18 was found to effectively inhibit the development of tumors and ascites, also resulting in long-term survival without significant toxic effect. CONCLUSIONS: Use of the short-range, high-linear energy transfer alpha-emitter At-211 conjugated to a surface epitope-recognizing monoclonal antibody appears to be highly efficient without significant toxicity in a mouse peritoneal tumor model, urging a Phase I clinical trial.
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| 6. |
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| 7. |
- Andersson, Martin, et al.
(författare)
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Multinationals in the Knowledge Economy : A case study of AstraZeneca in Sweden
- 2010
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Ingår i: Repositioning Europe and America for Growth. - Berlin : LIT Verlag. - 9783643107763
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Bokkapitel (refereegranskat)abstract
- This report presents a case study of the role of a large R&D intensive multinational company for a small open economy. The case study examines the role of AstraZeneca in the Swedish economy, i.e. an economy dominated by multinational companies. The purpose of the report is to analyze the interaction of AstraZeneca’s units in Sweden with the rest of the Swedish economy, and the Swedish innovation system in particular. We analyze the company’s role as an employer in the private sector, its transaction links with other Swedish firms and its role for Sweden’s exports. In a second perspective we focus on the company’s role in the Swedish knowledge economy and innovation system. The report analyses the company as a node for knowledge flows in the Swedish economy and innovation system, and its role as an employer of highly educated and skilled workers in Sweden.Our analysis of the Swedish units’ interaction with the rest of the Swedish economy shows that ’traditional’ couplings in the form of transactions with Swedish suppliers are limited. It is instead the company’s position in the ‘knowledge economy’ that makes its presence in Sweden important.
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| 8. |
- Andersson, Martin, et al.
(författare)
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Multinationals in the Knowledge Economy : A case study of AstraZeneca in Sweden
- 2008
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Multinational companies play a large and growing role in the world economy. Theycontribute about 10 percent to world GDP and about two thirds to global exports. Inthe vast majority of the countries in the world, the presence of multinationals has alsobeen growing over time. This report presents a case study of the role of a large multinational company, activein one of the most R&D and knowledge intensive industries of the world, withestablishments in a small open economy. The case study examines the role ofAstraZeneca in the Swedish economy, i.e. an economy dominated by multinationalcompanies. They account for almost all of Sweden’s aggregate investments in privateR&D, over 90 percent of the country’s exports and imports as well as a significantshare of the total number of employees in the private sector. The analyses in the reportmake it possible to assess the importance of the local presence of such a largeknowledge-intensive multinational for Sweden.
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| 9. |
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| 10. |
- Andersson, Martin, et al.
(författare)
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Preface
- 2013
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Ingår i: Innovation and Growth. - : Oxford University Press (OUP).
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Bokkapitel (refereegranskat)
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| 11. |
- Andersson, Martin, et al.
(författare)
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Reflections
- 2013
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Ingår i: Innovation and Growth. - : Oxford University Press. - 9780191748998 - 9780199646685
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Bokkapitel (refereegranskat)
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| 12. |
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| 13. |
- Biro, Thomas, et al.
(författare)
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Substrate Integrated Waveguide
- 2008
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Ingår i: Proceedings of RVK08/MMWP08, Växjö June 9-13, 2008. - : Växjö universitet. ; , s. 284-286
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- A substrate integrated waveguide, SIW, is a waveguide that is integrated directly on a subtrate. These structeres has recently been developed for different applications. The simplest SIW design is a rectangular waveguide in a subtrate formed by metallization of the upper and lower surfaces of the substrate and by placing vertical metal vias periodically along two parallel lines. Different types of SIW are analyzed and simulated in this contribution. In particular wave propagation along an SIW is analyzed.
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| 14. |
- Bjuggren, Per-Olof, et al.
(författare)
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Kommunal markskatt är mycket bättre
- 2006
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Ingår i: Svenska Dagbladet. - 1101-2412. ; 22 september
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 15. |
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| 16. |
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| 17. |
- Ebenhag, Sven-Christian, et al.
(författare)
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Coherent optical two-way frequency transfer in a commercial DWDM network
- 2016
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Ingår i: Proceedings of the Annual Precise Time and Time Interval Systems and Applications Meeting. - : Institute of Navigation. ; , s. 116-120, s. 116-120
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Konferensbidrag (refereegranskat)abstract
- An experimental fiber connection for ultra-stable optical frequency transfer is established between SP Technical Research Institute of Sweden in Borås and Chalmers University of Technology in Gothenburg. The distance is approximately 60 km, and the connection is implemented in the Swedish University Computer Network. The sites are connected through an active flexible communication network where each optical channel can be configured with terminal equipment based on the user needs. The network is implemented with unidirectional optical amplifiers and duplex fibers. The signal quality and the stability when sending an optical coherent frequency utilizing a wavelength in a DWDM system fiber pair, is evaluated within this work. The aim of the system is to be ultra-stable which corresponds to a stability of 10-13 for t = 1 s (Overlapping Allan Variance), as well as providing the ability to distribute monitored ultra-stable frequency with a future traceability to UTC (SP) to multiple users within the future network. This paper describes the current status and results from the frequency transfer between SP and Chalmers.
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| 18. |
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| 19. |
- Ekebergh, Andreas, 1984, et al.
(författare)
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Oxidative Coupling as a Biomimetic Approach to the Synthesis of Scytonemin
- 2011
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Ingår i: Organic Letters. - : American Chemical Society (ACS). - 1523-7052 .- 1523-7060. ; 13:16, s. 4458-4461
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Tidskriftsartikel (refereegranskat)abstract
- The first total synthesis of the dimeric alkaloid pigment scytonemin is described. The key transformations In Its synthesis from 3-indole acetic acid are a Heck carbocyclization and a Suzuki-Miyaura cross-coupling, orchestrated In a stereospecific tandem fashion, followed by a biosynthetically inspired oxidative dimerization. The tandem sequence generates a tetracyclic (E)-3-(arylidene)-3,4-dihydrocyclopenta[b]indol-2(1H)-one that is subsequently dimerized into the unique homodimeric core structure of scytonemin.
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| 20. |
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| 21. |
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| 22. |
- Elgqvist, Jörgen, 1963, et al.
(författare)
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Myelotoxicity and RBE of 211At-conjugated monoclonal antibodies compared with 99mTc-conjugated monoclonal antibodies and 60Co irradiation in nude mice
- 2005
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Ingår i: J Nucl Med. - 0161-5505. ; 46:3, s. 464-71
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Tidskriftsartikel (refereegranskat)abstract
- The rationale of this study was to determine the myelotoxicity in nude mice of the alpha-emitter 211At conjugated to monoclonal antibodies (mAbs) and to compare the effect with an electron emitter, (99m)Tc, and external irradiation from a 60Co source, for estimation of the relative biological effectiveness (RBE). METHODS: 211At and (99m)Tc were conjugated to the IgG1 mAbs MX35 and 88BV59. Nude female BALB/c mice, 8- to 12-wk old, were injected intraperitoneally or intravenously. The biodistribution was determined 3, 6, and 18 h after injection. The bone-to-blood and bone marrow-to-blood activity concentration ratios (BBLR and BMBLR, respectively) were determined for simultaneously injected 211At- and (99m)Tc-mAbs. Bone marrow samples were taken from the femur. For each mouse, the whole-body retention was measured as well as the blood activity by repeated blood samples from the tail vein (0), 1, 3, 6, 12, and 18 h after injection. External-beam irradiation from a 60Co source was also performed at 3 different dose levels. White blood cell (WBC) counts, red blood cell counts, platelet counts, and hemoglobin were determined for each mouse initially and on days 1, 4, 5, 7, 15, 22, and 27 after injection. The calculations of the absorbed dose to the bone marrow were based on the BBLR, BMBLR, the cumulated activities, and the absorbed fractions. The absorbed fractions, phi, for alpha-particles and electrons in the bone marrow were calculated using Monte Carlo simulations based on a bone marrow dosimetry model. RESULTS: The BMBLR was 0.58 +/- 0.06 and 0.56 +/- 0.06 for the 211At- and (99m)Tc-mAbs, respectively. No significant variation in BMBLR with time was found. The absorbed fractions for alpha-particles and electrons in the bone marrow were 0.88 and 0.75, respectively. The mean absorbed fractions of the photons from (99m)Tc were 0.033 and 0.52 for 140 and 18.3 keV, respectively. When different amounts of 211At- and (99m)Tc-mAbs (0.09-1.3 and 250-1,300 MBq, respectively) were administered intraperitoneally or intravenously, corresponding to absorbed doses to the bone marrow of 0.01-0.60 and 0.39-1.92 Gy, respectively, the WBC counts was suppressed by 1%-90% and 23%-89%, respectively. When external-beam irradiation with a 60Co source was performed to absorbed doses of 1.4, 1.9, and 2.4 Gy, the WBC counts was suppressed by 47%-90%. These results indicate a myelotoxic in vivo RBE of 3.4 +/- 0.6 for alpha-particles compared with (99m)Tc and 5.0 +/- 0.9 compared with 60Co irradiation. CONCLUSION: The effect on the WBC counts from bone marrow irradiation with 211At-mAbs indicates an in vivo RBE of 3.4 +/- 0.6 in comparison with (99m)Tc-mAbs. The RBE value compared with external irradiation is 5.0 +/- 0.9.
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| 23. |
- Elgqvist, Jörgen, 1963, et al.
(författare)
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Therapeutic efficacy and tumor dose estimations in radioimmunotherapy of intraperitoneally growing OVCAR-3 cells in nude mice with (211)At-labeled monoclonal antibody MX35
- 2005
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Ingår i: J Nucl Med. - 0161-5505. ; 46:11, s. 1907-15
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Tidskriftsartikel (refereegranskat)abstract
- The purpose of this study was to investigate the therapeutic efficacy of-and to estimate the absorbed dose to-tumor cells from radioimmunotherapy (RIT) in an ovarian cancer model using the alpha-particle-emitting nuclide (211)At labeled to monoclonal antibody (mAb) MX35. Previous studies on mAb MOv18 did not allow for dosimetry because of antigen shedding in vitro. METHODS: Five-week-old female nude BALB/c nu/nu mice were inoculated intraperitoneally with 1 x 10(7) cells of the human tumor cell line OVCAR-3. Three weeks later, the animals were given approximately 400, 800, or 1,200 kBq of (211)At-labeled mAb MX35 intraperitoneally. As controls, one group of animals was injected with unlabeled mAb and another group was injected with phosphate-buffered saline (PBS). Another group was given approximately 400 kBq of (211)At labeled to the previously investigated mAb MOv18 for efficacy comparison. Two months after treatment, the animals were sacrificed and the presence of macroscopic and microscopic tumors, as well as ascites, was determined. The absorbed dose to tumor cells on the peritoneal surface was estimated in terms of the sum of a specific and a nonspecific contribution. The specific contribution, arising from mAbs binding to the antigenic sites on the cell membrane, was calculated using a dynamic compartment model developed in-house and Monte Carlo software. The model used as input values the number of mAbs injected into the abdominal cavity, N(mAb), the specific activity, A(sp), the association rate constant, k(on), and the maximal number of mAbs bound per cell, B(max)-all determined by in vitro experiments. This specific component of the absorbed dose was calculated for assumed cell cluster sizes with radii of 25, 50, and 100 microm. The nonspecific contribution to the absorbed dose was derived from unbound mAbs freely circulating in the abdominal cavity, also using the Monte Carlo software. RESULTS: In the control groups given unlabeled MX35 or PBS, all 18 animals had ascites, 6 of 9 animals in each group had macroscopic tumors, and all animals had microscopic growth. In the 3 groups given different amounts of (211)At-MX35, only 3 of 25 animals developed ascites. None of these animals had any sign of macroscopic tumors, but 8 had microscopic growth. In the group given (211)At-MOv18, no animals had ascites or macroscopic tumors, but 3 of 10 animals had microscopic tumors. After injecting 400 kBq of (211)At-MX35, the absorbed dose due to specific binding, for a cell cluster with a radius of 50 microm, ranged from 413 to 223 Gy between 0- and 45-microm distance from the cluster center, assuming a homogeneous distribution of (211)At-MX35 in the cluster. The contribution from unbound (211)At-MX35 and (211)At-MX35 only distributed on the cluster surface, for this cluster size, ranged from 7 to 14 Gy and from 29 to 94 Gy, between 0- and 45-microm distance from the cluster center, respectively. The calculated total absorbed doses are in a clinically relevant range and were effective as verified in the nude mice with subclinical intraperitoneal growth of OVCAR-3 cells. CONCLUSION: (211)At-MX35 injected intraperitoneally exhibits a high efficacy when treating micrometastatic growth of the ovarian cancer cell line OVCAR-3 on the peritoneum of nude mice.
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| 24. |
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| 25. |
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| 26. |
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| 27. |
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| 28. |
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| 29. |
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Entrepreneurship and regional development : Local processes and global patterns
- 2010
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Samlingsverk (redaktörskap) (refereegranskat)abstract
- 'The world is experiencing the fourth globalization trend since the collapse of the Berlin Wall twenty years ago. This trend unlike previous ones is characterized by both broader global interconnection and deeper localization. In other words, the world is both flatter and spikier at the same time. The key to a successful development policy is to integrate these two seemingly counter intuitive trends. The solution to this is a more or less regional strategy with a very strong focus on entrepreneurship. While this approach is not new and is not the first, it is the best one that I have seen. The editors of this collection are some of the best informed, most careful and deep thinking scholars in the business and have produced a work worthy of their stature.
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| 30. |
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Entrepreneurship, social capital and governance : Directions for the sustainable development and competitiveness of regions
- 2012. - 1
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Samlingsverk (redaktörskap) (refereegranskat)abstract
- This book highlights the role of entrepreneurship, social capital and governance for regional economic development. In recent decades, many researchers have claimed that entrepreneurship is the most critical factor in sustaining regional economic growth. However, most entrepreneurship research is undertaken without considering the fundamental importance of the regional context. Other research has emphasized the role of social capital but there are substantial problems in empirically relating measures of social capital to regional economic development. © Charlie Karlsson, Börje Johansson and Roger R. Stough 2012. All rights reserved.
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| 31. |
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| 32. |
- Gustafsson, Stefan, et al.
(författare)
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Electromagnetic dispersion modeling and measurements for HVDC power cables
- 2014
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Ingår i: IEEE Transactions on Power Delivery. - : IEEE Press. - 0885-8977 .- 1937-4208. ; 29:6, s. 2439-2447
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Tidskriftsartikel (refereegranskat)abstract
- This paper provides a general framework for electromagnetic (EM) modeling, sensitivity analysis, computation, and measurements regarding the wave propagation characteristics of high-voltage direct-current (HVDC) power cables. The modeling is motivated by the potential use with transient analysis, partial-discharge measurements, fault localization and monitoring, and is focused on very long (10 km or more) HVDC power cables with transients propagating in the low-frequency regime of about 0-100 kHz. An exact dispersion relation is formulated together with a discussion on practical aspects regarding the computation of the propagation constant. Experimental time-domain measurement data from an 80-km-long HVDC power cable are used to validate the electromagnetic model, and a mismatch calibration procedure is devised to account for the connection between the measurement equipment and the cable. Quantitative sensitivity analysis is devised to study the impact of parameter uncertainty on wave propagation characteristics. The sensitivity analysis can be used to study how material choices affect the propagation characteristics, and to indicate which material parameters need to be identified accurately in order to achieve accurate fault localization. The analysis shows that the sensitivity of the propagation constant due to a change in the conductivity in the three metallic layers (the inner conductor, the intermediate lead shield, and the outer steel armor) is comparable to the sensitivity with respect to the permittivity of the insulating layer. Hence, proper modeling of the EM fields inside the metallic layers is crucial in the low-frequency regime of 0-100 kHz.
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| 33. |
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| 34. |
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| 35. |
- Ingelsten, Madeleine, 1978, et al.
(författare)
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Is Indoleamine 2,3-Dioxygenase Important for Graft Acceptance in Highly Sensitized Patients After Combined Auxiliary Liver-Kidney Transplantation?
- 2009
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Ingår i: Transplantation. - 0041-1337. ; 88:7, s. 911-919
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Tidskriftsartikel (refereegranskat)abstract
- Background. In the clinical setting, transplanted liver seems to protect other grafts from the same donor from rejection. Our previous findings suggest that an auxiliary liver transplantation a few hours before a renal transplantation not only inhibits hyperacute antibody-mediated rejection but also improves long-term kidney graft survival in sensitized recipients. Here, we investigated indoleamine 2,3-dioxygenase (IDO) activity, as one potential mechanism for liver-induced long-term acceptance of kidney grafts. Methods. Tryptophan degradation was measured to estimate IDO activity in patient sera and cell culture supernatants with high performance liquid chromatography. Gene expression in the grafted organs and cell lysates was studied using real time polymerase chain reaction analysis. Results. Tryptophan degradation increased in peripheral blood from patients undergoing combined auxiliary liver-kidney transplantation, whereas it decreased in patients after regular renal transplantation. A 100-fold increase in IDO mRNA, preceded by upregulation of the IDO-inducing cytokines tumor necrosis factor-[alpha], interleukin-1[beta], and interferon-[gamma], was observed in the transplanted organs after graft reperfusion in patients undergoing combined graft transplantation. Subsequent studies in vitro revealed that immature dendritic cells, but not hepatocytes, strongly activated IDO on maturation with tumor necrosis factor-[alpha], interleukin-1[beta], and interferon-[gamma]. Finally, serum from liver-transplanted patients elicited an even stronger IDO-activity in such cytokine-stimulated dendritic cells. Conclusions. Taken together these findings suggest that the liver-induced long-term acceptance seen in human combined auxiliary liver and kidney transplantation is at least partly mediated by IDO activity. (C) 2009 Lippincott Williams & Wilkins, Inc.
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| 36. |
- Ingelsten, Madeleine, 1978, et al.
(författare)
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Liver-derived IL-10 as a potential inhibitor of a TH1-deviating crosstalk between donor DC and recipient NK cells during combined liver-kidney transplantation
- 2009
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Ingår i: The Transplantation society XI Basic science symposium, European society for organ transplantation I Basic science meeting, Brussels 2009..
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Konferensbidrag (refereegranskat)abstract
- During transplantation DCs within the allograft become activated and migrate to secondary lymphoid organs (SLO) where they activate NK cells and alloreactive T cell. Previous findings suggest that a liver graft is less prone to induce rejection compared to a renal graft but the mechanisms are still unclear. We compared changes in graft gene expression of inflammatory mediators known to participate in transplantation-induced DC activation in the liver and kidney in patients undergoing combined liver-kidney transplantation (n=8). Real-time PCR analysis revealed a similar up-regulation of mRNA for the DC-activation-associated inflammatory mediators TNF-α, IL-1β and IFN-gamma after reperfusion in the liver and kidney. While no reperfusion-dependent mRNA increase for IL-10 was found in transplated grafts, serial measurement of cytokine levels in peripheral blood revealed that IL-10 levels increased 60-fold in serum shortly (within 1 h) after liver, but before kidney, reperfusion and returned to pre-transplant levels at day 2 post-transplantation (n=3). No significant changes were seen in IL-12 levels. DCs matured with a cocktail consisting of IFN-γ, TNF-α and IL-1β demonstrated a strong and sustained production of CXCR3-ligands which are indispensable for DC-mediated recruitment of blood NK cells into SLO and their subsequent boosting of TH1-deviated alloresponses. Addition of IL-10 during DC maturation significantly decreased the production of all three CXCR3-ligands (CXCL9, CXCL10 and CXCL11). These findings indicate that IL-10 is selectively and rapidly released during liver transplantation, probably due to accumulation during the ischehmia period. This release may potentially inhibit a TH1-deviating crosstalk between donor DC and recipient NK cells.
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| 37. |
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| 38. |
- Ingelsten, Madeleine, 1978, et al.
(författare)
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Postischemic Inflammatory Response in an Auxiliary Liver Graft Predicts Renal Graft Outcome in Sensitized Patients
- 2011
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Ingår i: Transplantation. - 0041-1337 .- 1534-6080. ; 91:8, s. 888-894
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Tidskriftsartikel (refereegranskat)abstract
- Background. The liver is considered a tolerogenic organ that favors the induction of peripheral tolerance and protects other organs from the same donor from rejection. This has been exploited in combined auxiliary liver-kidney transplantation, where a renal graft is transplanted against a positive crossmatch under the protection of a liver transplanted from the same donor. Methods. To elucidate mechanisms behind the liver protective effect, we studied early transcriptional changes of inflammatory mediators in the grafts during combined auxiliary liver-kidney transplantation using microarrays and real-time polymerase chain reaction. The results were correlated to clinical data. Results. Liver and kidney grafts both exhibited an upregulation of the leukocyte-recruiting chemokines CCL2, CCL3, and CCL4. Notably, liver grafts strongly upregulated CCL20, a dendritic cell, and T-cell recruiting chemokine. By comparing the gene expression in liver grafts with the clinical outcome, we found that 14 of 45 investigated inflammatory genes were expressed significantly higher in patients without early rejection when compared with those with early rejections. This included the above-mentioned chemokines and the T-cell-recruiting CX3CL1, NFKB1, and the tolerance-inducing gene indoleamine 2,3-dioxygenase. Conclusions. In this study, the protective role of the liver was associated with a proinflammatory reaction within this organ after ischemia-reperfusion. In particular, we found an increased expression of leukocyte-recruiting chemokines in patients without rejection, indicating a protective role of host inflammatory cells infiltrating the auxiliary liver graft in presensitized patients. Second, gene expression profiling of transplant biopsies shortly after reperfusion predicted the risk of early rejection in these patients.
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| 39. |
- Ingelsten, Madeleine, 1978, et al.
(författare)
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Rapid Increase of Interleukin-10 Plasma Levels After Combined Auxiliary Liver-Kidney Transplantation in Presensitized Patients
- 2014
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Ingår i: Transplantation. - : Ovid Technologies (Wolters Kluwer Health). - 0041-1337. ; 98:2, s. 208-215
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Tidskriftsartikel (refereegranskat)abstract
- Background. After transplantation, donor dendritic cells (DCs) in the grafted organ are activated by an ischemia/reperfusion-induced inflammatory process that induces their migration to the recipient's secondary lymphoid tissues. The subsequent interaction between migrated and mature donor DCs, recipient T cells, and natural killer (NK) cells is proposed to be crucial in directing host immune reactions toward allograft rejection. A liver transplant is less prone to induce rejection compared with most other solid organ transplants, and simultaneous transplantation of liver and kidney is known to improve the clinical outcome of kidney transplantation. Methods and Results. Here we show that liver as well as combined auxiliary liver-kidney transplantation in patients induces a rapid increase in plasma interleukin-10 (IL-10) to levels that are significantly higher than those seen after standard kidney transplantation. Addition of IL-10 during in vitro maturation of human monocyte-derived DCs with ischemia/reperfusion-associated factors was found to affect phenotypic DC maturation significantly. Addition of IL-10 inhibited DC production of the NK cell- and T cell-recruiting chemokines CXCL9, CXCL10 and CXCL11. Conclusion. Our findings indicate that liver transplantation induces a substantial systemic release of IL-10, which may inhibit T cell- and NK cell- mediated rejection processes toward the transplanted liver and concurrently transplanted kidney.
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| 40. |
- Ingelsten, Madeleine, 1978, et al.
(författare)
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The role of IL-10 in the liver tolerance effect
- 2009
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Ingår i: World congress of Nephrology, Milan 2009.
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Konferensbidrag (refereegranskat)abstract
- Introduction and aim: A liver graft is less prone to induce rejection compared to a renal graft and previous findings suggest that an auxiliary liver transplantation a few hours before a renal transplantation improves kidney graft survival in highly sensitized patients. Dendritic cells (DC) are important initiators of rejection. During transplantation DC within the allograft become activated and migrate to secondary lymphoid organs where they activate NK cells and alloreactive T cell. The aim of this study was to investigate the role of DC maturation in the liver mediated acceptance of a renal graft. Methods: Real-time PCR was used to compare changes in graft gene expression of IFN-γ, TNF-α, IL-1β and IL-10 in the liver and kidney in patients undergoing combined auxiliary liver-kidney transplantation (n=8). These are inflammatory mediators known to participate in transplantation-induced DC activation. Presence of interleukin (IL) 10 and 12 was measured in peripheral blood in these patients. Monocyte-derived DCs was matured with a cytokine-cocktail consisting of IFN-γ, TNF-α and IL-1β +/- IL-10. The effect of these cytokines on DC secretion of chemokines MIG, IP-10 and I-TAC was investigated with ELISA. Results: The DC-activation-associated inflammatory mediators TNF-α, IL-1β and IFN-γ were similarly upregulated in both liver and kidney grafts after reperfusion. While no reperfusion-dependent mRNA increase for IL-10 was found in transplated grafts, serial measurement of cytokine levels in peripheral blood revealed that IL-10 levels increased 60-fold in serum shortly (within 1 h) after liver, but before kidney, reperfusion and returned to pre-transplant levels at day 2 post-transplantation (n=3). No significant changes were seen in IL-12 levels. DCs matured with a cocktail consisting of IFN-γ, TNF-α and IL-1β demonstrated a strong production of CXCR3-ligands (MIG 280 ng/ml, IP-10 19 ng/ml, I-TAC 619 pg/ml) which was sustained even after removal of the cytokine-cocktail. These chemokines are indispensable for DC-mediated recruitment of blood NK cells into secondary lymphoid organs and their subsequent boosting of TH1-deviated alloresponses. Addition of IL-10 during DC maturation significantly decreased the production of all three CXCR3-ligands (MIG -33%, IP-10 -25%, I-TAC -67%). Conclusions: These findings indicate that IL-10 is selectively and rapidly released during liver transplantation, probably due to accumulation during the ischehmia period. This release may potentially inhibit a TH1-deviating crosstalk between donor DC and recipient NK cells, suggesting a role for IL-10 in the liver-mediated acceptance of a renal graft in highly sensitized patients.
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| 41. |
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| 42. |
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Innovation, Agglomeration and Regional Competition
- 2009
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Samlingsverk (redaktörskap) (refereegranskat)abstract
- 'The rise of globalization has triggered a fundamental rethinking about the role of regions in economic development policy. In this important new book, Karlsson, Johansson and Stough assemble a cast of leading international scholars to unravel the new role for regions and local economic development policy to harness the possibilities unleashed by the forces of globalization. This book contains important new insights and ideas that will be welcomed by both scholars and policymakers.
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| 43. |
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Innovation and Growth : From R&D strategies of innovating firms to economy-wide technological change
- 2013
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Samlingsverk (redaktörskap) (övrigt vetenskapligt/konstnärligt)abstract
- This volume provides an understanding of firms' R&D and innovation strategies and their economy-wide consequences. It is based on the premise that differences in firm-level returns, as well as economy-wide outcomes, may be linked to the heterogeneous ways in which firms organize and undertake R&D and innovation activities. It emphasizes innovation strategies of innovating firms, and reflects that innovation efforts do not represent a uniform type of expenditure. Organized into three parts the volume moves from the micro to the macro-level. This structure highlights the notion that R&D and innovation and growth are two interdependent perspectives. The first of these is micro-oriented and focuses on innovation processes of firms, where R&D activities and other innovation efforts give rise to consequences such as a strengthening of resource bases, growth of sales and employment, patents, new products, increasing productivity and profits, and improved chances of survival. The second perspective comprises economy-wide effects in the form of overall technological change, growth in total factor productivity, and structural change processes, where certain sectors may benefit from new inputs from other sectors while others experience declining markets and reduced output.
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| 44. |
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| 45. |
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| 46. |
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| 47. |
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| 48. |
- Johansson, Börje, et al.
(författare)
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Entrepreneurship and Development - local processes and global patterns
- 2009
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The general motivation for this paper is the current interest in globalization as a phenomenon that strongly affects the conditions of local economic development. Our purpose is to contribute to some of the current development aspects, in particular those that foster the evolution of entrepreneurs in local-global processes. We present four eras of globalization, in recent decades and which have been described as different aspects of globalization are not new at all. In conclusion, we stress that those global patterns of change that are observed, and reported in the media and by social scientists are the result of innumerable local processes driven by economic, political and social entrepreneurs in localities, regions, and nations all around the globe.
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| 49. |
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| 50. |
- Johansson, Börje, et al.
(författare)
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Entrepreneurship and Innovation in Functional Regions
- 2008
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The purpose of this paper is to discuss the role of entrepreneurship and innovations foreconomic development in functional regions and in doing that highlighting the differentconditions offered for entrepreneurship and innovations in functional regions of various sizes.In conclusion, the conditions for entrepreneurship and innovations vary substantially betweenfunctional regions, since the necessary knowledge resources tend to be local and to cluster incertain regions and not others. Functional regions with a high capacity to generate new ideas,create knowledge, organizational learning and innovations are characterized as learningregions. Large functional regions offer a large market potential and a superior accessibility toknowledge and knowledge resources and they will further develop their creative capabilitiesdue to an accumulation of innovative and entrepreneurial knowledge.
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