| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
- Bajpai, Anju, et al.
(författare)
-
Isoforms diversity and transcriptional regulation of floral meristem identity and pathway integrators, driving floral transition in mango under subtropics
- 2024
-
Ingår i: Scientia Horticulturae. - 0304-4238. ; 334
-
Tidskriftsartikel (refereegranskat)abstract
- Floral initiation in mango is intricately regulated by a complex genetic network that integrates diverse environmental and endogenous cues. The flowering patterns in different varieties contingent upon their genetic network and sensitivity to the environmental signals, culminating in the phenomenon of "alternate bearing in several mango varieties. In this study, we conducted a comprehensive molecular dissection of the genetic regulators of flowering through comparative transcriptomics in mango varieties having contrast flowering pattern traits. A total of 45 and 54 million 150 base-paired reads were obtained and assembled in 58,094 and 50,945 transcripts for Amrapali and Chausa, respectively. Among them several candidates were identified as flowering regulators, operating at multilevel like floral meristem transition (SUPPRESSOR OF OVER-EXPRESSION OF CONSTANS, MiSOC1, and APETELA, MiAP1 and MiAP2), floral signal integrators (CONSTANS- LIKE, MiCOL; AGAMOUS LIKE MiAGL and SQUAMOSA PROMOTER BINDING PROTEIN LIKE, MiSPL), hormonal regulation (S-adenosyl methionine synthase, MiSAMS), cell division and cell cycle regulators (Cyclin dependant kinase, MiCDK5 and SIN-associated polypeptide, MiSAP18). Moreover, multiple isoforms of these flowering genes were also identified in these varieties. qRT-PCR analysis revealed differential transcriptional regulation of these multiple isoforms in floral and vegetative buds in the contrasting varieties. Furthermore, by investigating the gene expression of these multiple isoforms in various mango varieties with different flowering pattern traits, we established the role of these isoforms in mango flowering. Finally, gene expression findings supported by protein motif analysis and phylogenetic insights facilitated the development of a comprehensive flowering model that may applicable to subtropical ecology.
|
|
| 11. |
- Broda, Martyna, et al.
(författare)
-
Increased expression of ANAC017 primes for accelerated senescence
- 2021
-
Ingår i: Plant Physiology. - : Oxford University Press (OUP). - 0032-0889 .- 1532-2548. ; 186:4, s. 2205-2221
-
Tidskriftsartikel (refereegranskat)abstract
- Recent studies in Arabidopsis (Arabidopsis thaliana) have reported conflicting roles for NAC DOMAIN CONTAINING PROTEIN 17 (ANAC017), a transcription factor regulating mitochondria-to-nuclear signaling, and its closest paralog NAC DOMAIN CONTAINING PROTEIN 16 (ANAC016), in leaf senescence. By synchronizing senescence in individually darkened leaves of knockout and overexpressing mutants from these contrasting studies, we demonstrate that elevated ANAC017 expression consistently causes accelerated senescence and cell death. A time-resolved transcriptome analysis revealed that senescence-associated pathways such as autophagy are not constitutively activated in ANAC017 overexpression lines, but require a senescence-stimulus to trigger accelerated induction. ANAC017 transcript and ANAC017-target genes are constitutively upregulated in ANAC017 overexpression lines, but surprisingly show a transient "super-induction" 1 d after senescence induction. This induction of ANAC017 and its target genes is observed during the later stages of age-related and dark-induced senescence, indicating the ANAC017 pathway is also activated in natural senescence. In contrast, knockout mutants of ANAC017 showed lowered senescence-induced induction of ANAC017 target genes during the late stages of dark-induced senescence. Finally, promoter binding analyses show that the ANAC016 promoter sequence is directly bound by ANAC017, so ANAC016 likely acts downstream of ANAC017 and is directly transcriptionally controlled by ANAC017 in a feed-forward loop during late senescence.
|
|
| 12. |
- Darwish, Essam, et al.
(författare)
-
Touch signaling and thigmomorphogenesis are regulated by complementary CAMTA3- and JA-dependent pathways
- 2022
-
Ingår i: Science Advances. - : American Association for the Advancement of Science (AAAS). - 2375-2548. ; 8:20
-
Tidskriftsartikel (refereegranskat)abstract
- Plants respond to mechanical stimuli to direct their growth and counteract environmental threats. Mechanical stimulation triggers rapid gene expression changes and affects plant appearance (thigmomorphogenesis) and flowering. Previous studies reported the importance of jasmonic acid (JA) in touch signaling. Here, we used reverse genetics to further characterize the molecular mechanisms underlying touch signaling. We show that Piezo mechanosensitive ion channels have no major role in touch-induced gene expression and thigmomorphogenesis. In contrast, the receptor-like kinase Feronia acts as a strong negative regulator of the JA-dependent branch of touch signaling. Last, we show that calmodulin-binding transcriptional activators CAMTA1/2/3 are key regulators of JA-independent touch signaling. CAMTA1/2/3 cooperate to directly bind the promoters and activate gene expression of JA-independent touch marker genes like TCH2 and TCH4. In agreement, camta3 mutants show a near complete loss of thigmomorphogenesis and touch-induced delay of flowering. In conclusion, we have now identified key regulators of two independent touch-signaling pathways.
|
|
| 13. |
- Tran, Huy Cuong, et al.
(författare)
-
An mTRAN-mRNA interaction mediates mitochondrial translation initiation in plants
- 2023
-
Ingår i: Science. - 1095-9203. ; 381:6661
-
Tidskriftsartikel (refereegranskat)abstract
- Plant mitochondria represent the largest group of respiring organelles on the planet. Plant mitochondrial messenger RNAs (mRNAs) lack Shine-Dalgarno-like ribosome-binding sites, so it is unknown how plant mitoribosomes recognize mRNA. We show that “mitochondrial translation factors” mTRAN1 and mTRAN2 are land plant–specific proteins, required for normal mitochondrial respiration chain biogenesis. Our studies suggest that mTRANs are noncanonical pentatricopeptide repeat (PPR)–like RNA binding proteins of the mitoribosomal “small” subunit. We identified conserved Adenosine (A)/Uridine (U)-rich motifs in the 5′ regions of plant mitochondrial mRNAs. mTRAN1 binds this motif, suggesting that it is a mitoribosome homing factor to identify mRNAs. We demonstrate that mTRANs are likely required for translation of all plant mitochondrial mRNAs. Plant mitochondrial translation initiation thus appears to use a protein-mRNA interaction that is divergent from bacteria or mammalian mitochondria.
|
|
| 14. |
- Tyagi, Gunjan, et al.
(författare)
-
Light Responsiveness and Assembly of Arylazopyrazole-Based Surfactants in Neat and Mixed CTAB Micelles
- 2022
-
Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863. ; 2:12, s. 2670-2677
-
Tidskriftsartikel (refereegranskat)abstract
- The self-assembly of an arylazopyrazole-based photosurfactant (PS), based on cetyltrimethylammonium bromide (CTAB), and its mixed micelle formation with CTAB in aqueous solution was investigated by small angle neutron and X-ray scattering (SANS/SAXS) and UV-vis absorption spectroscopy. Upon UV light exposure, PS photoisomerizes from E-PS (trans) to Z-PS (cis), which transforms oblate ellipsoidal micelles into smaller, spherical micelles with larger shell thickness. Doping PS with CTAB resulted in mixed micelle formation at all stoichiometries and conditions investigated; employing selectively deuterated PS, a monotonic variation in scattering length density and dimensions of the micellar core and shell is observed for all contrasts. The concentration- and irradiance-dependence of the E to Z configurational transition was established in both neat and mixed micelles. A liposome dye release assay establishes the enhanced efficacy of photosurfactants at membrane disruption, with E-PS exhibiting a 4-fold and Z-PS a 10-fold increase in fluorescence signal with respect to pure CTAB. Our findings pave the way for external triggering and modulation of the wide range of CTAB-based biomedical and material applications.
|
|
| 15. |
|
|
