| 1. |
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| 2. |
- Yoo, Taekyeong, et al.
(författare)
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Disease-specific eQTL screening reveals an anti-fibrotic effect of AGXT2 in non-alcoholic fatty liver disease.
- 2021
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Ingår i: Journal of hepatology. - : Elsevier BV. - 1600-0641 .- 0168-8278. ; 75:3, s. 514-523
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Tidskriftsartikel (refereegranskat)abstract
- Nonalcoholic fatty liver disease (NAFLD) poses an impending clinical burden. Genome-wide association studies have revealed a limited contribution of genomic variants to the disease, requiring alternative but robust approaches to identify disease-associated variants and genes. We carried out a disease-specific expression quantitative trait loci (eQTL) screen to identify novel genetic factors that specifically act on NAFLD progression on the basis of genotype.We recruited 125 Korean biopsy-proven NAFLD patients and healthy individuals and performed eQTL analyses using 21,272 transcripts and 3,234,941 genotyped and imputed SNPs. We then selected eQTLs that were detected only in the NAFLD group, but not in the control group (i.e., NAFLD-eQTLs). An additional cohort of 162 Korean NAFLD individuals was used for replication. The function of the selected eQTL toward NAFLD development was validated using HepG2, primary hepatocytes and NAFLD mouse models.The NAFLD-specific eQTL screening yielded 242 loci. Among them, AGXT2, encoding alanine-glyoxylate aminotransferase 2, displayed decreased expression in NAFLD patients homozygous for the non-reference allele of rs2291702, compared to no-NAFLD subjects with the same genotype (P = 4.79 × 10-6). This change was replicated in an additional 162 individuals, yielding a combined P-value of 8.05 × 10-8 from a total of 245 NAFLD patients and 48 controls. Knockdown of AGXT2 induced palmitate-overloaded hepatocyte death by increasing ER stress, and exacerbated NAFLD diet-induced liver fibrosis in mice. However, overexpression of AGXT2 reversely attenuated liver fibrosis and steatosis as well.We implicate a new molecular role of AGXT2 in NAFLD. Our overall approach will serve as an efficient tool for uncovering novel genetic factors that contribute to liver steatosis and fibrosis in patients with NAFLD.Elucidating causal genes for NAFLD has been challenging due to limited tissue availability and the polygenic nature of the disease. Using liver and blood samples from 125 biopsy-proven NAFLD and no-NAFLD Korean individuals and an additional 162 individuals for replication, we devised a new analytic method to identify causal genes. Among the candidates, we found that AGXT2-rs2291702 protects against liver fibrosis in a genotype-dependent manner with the potential for therapeutic interventions. Our approach enables the discovery of NAFLD causal genes that act on the basis of genotype.
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| 3. |
- Cheon, Jae Yeong, et al.
(författare)
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Ordered mesoporous porphyrinic carbons with very high electrocatalytic activity for the oxygen reduction reaction
- 2013
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 3, s. 2715-
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Tidskriftsartikel (refereegranskat)abstract
- The high cost of the platinum-based cathode catalysts for the oxygen reduction reaction (ORR) has impeded the widespread application of polymer electrolyte fuel cells. We report on a new family of non-precious metal catalysts based on ordered mesoporous porphyrinic carbons (M-OMPC; M = Fe, Co, or FeCo) with high surface areas and tunable pore structures, which were prepared by nanocasting mesoporous silica templates with metalloporphyrin precursors. The FeCo-OMPC catalyst exhibited an excellent ORR activity in an acidic medium, higher than other non-precious metal catalysts. It showed higher kinetic current at 0.9 V than Pt/C catalysts, as well as superior long-term durability and MeOH-tolerance. Density functional theory calculations in combination with extended X-ray absorption fine structure analysis revealed a weakening of the interaction between oxygen atom and FeCo-OMPC compared to Pt/C. This effect and high surface area of FeCo-OMPC appear responsible for its significantly high ORR activity.
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| 4. |
- Choi, Joong Il Jake, et al.
(författare)
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Atomic-scale view of stability and degradation of single-crystal MAPbBr(3) surfaces
- 2019
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Ingår i: Journal of Materials Chemistry A. - : Royal Society of Chemistry (RSC). - 2050-7488 .- 2050-7496. ; 7:36, s. 20760-20766
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Tidskriftsartikel (refereegranskat)abstract
- While organic-inorganic hybrid perovskite solar cells are emerging as promising candidates for next-generation solar cells with fascinating power conversion efficiency, the instability of perovskites remains a significant bottleneck for their commercialization. An atomic scale understanding of the degradation of hybrid perovskites, however, is only in its beginning stages because of the difficulty in preparing well-defined surface conditions for characterization. Using atomic force microscopy at ultra-high vacuum and room temperature, we report the first direct observation of the degradation process of a cleaved methylammonium lead bromide, MAPbBr(3) (MA: CH3NH3+), single crystal. Upon in situ cleavage, atomic force microscopy images show large flat terraces with monolayer height steps, which correspond to the surface of cubic MAPbBr(3) with methylammonium ligand termination. While this surface can be prepared via the cleavage process and is energetically stable, we observe that after several weeks under dark and vacuum conditions it degrades and produces clusters surrounded by pits. Guided by density functional theory calculations, we propose a degradation pathway that initiates even at low humidity levels and leads to the formation of surface PbBr2 species. We finally identify the electronic structure of the MA-bromine-terminated flat surface and find that it is correlated with a strong field-induced degradation of the MAPbBr(3) only at positive sample bias voltages.
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| 5. |
- JEONG, SJ, et al.
(författare)
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Potential impact of vegetation feedback on European heat waves in a 2 x CO 2 climate: Vegetation impact on European heat waves
- 2010
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Ingår i: Climatic Change. - : Springer Science and Business Media LLC. - 0165-0009 .- 1573-1480. ; 99:3-4, s. 625-635
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Tidskriftsartikel (refereegranskat)abstract
- Inclusion of the effects of vegetation feedback in a global climate change simulation suggests that the vegetation–climate feedback works to alleviate partially the summer surface warming and the associated heat waves over Europe induced by the increase in atmospheric CO2 concentrations. The projected warming of 4°C over most of Europe with static vegetation has been reduced by 1°C as the dynamic vegetation feedback effects are included.. Examination of the simulated surface energy fluxes suggests that additional greening in the presence of vegetation feedback effects enhances evapotranspiration and precipitation, thereby limiting the warming, particularly in the daily maximum temperature. The greening also tends to reduce the frequency and duration of heat waves. Results in this study strongly suggest that the inclusion of vegetation feedback within climate models is a crucial factor for improving the projection of warm season temperatures and heat waves over Europe
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| 6. |
- Chen, Deliang, 1961, et al.
(författare)
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Recent Recovery of the Siberian High Intensity
- 2011
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Ingår i: Journal of Geophysical Research. - 0148-0227 .- 2156-2202. ; 116
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Tidskriftsartikel (refereegranskat)abstract
- This study highlights the fast recovery of the wintertime Siberian High intensity (SHI) over the last two decades. The SHI showed a marked weakening trend from the 1970s to 1980s, leading to unprecedented low SHI in the early 1990s according to most observational data sets. This salient declining SHI trend, however, was sharply replaced by a fast recovery over the last two decades. Since the declining SHI trend has been considered as one of the plausible consequences of climate warming, the recent SHI recovery seemingly contradicts the continuous progression of climate warming in the Northern Hemisphere. We suggest that alleviated surface warming and decreased atmospheric stability in the central Siberia region, associated with an increase in Eurasian snow cover, in the recent two decades contributed to this rather unexpected SHI recovery. The prominent SHI change, however, is not reproduced by general circulation model (GCM) simulations used in the IPCC AR4. The GCMs indicate the steady weakening of the SHI for the entire 21st century, which is found to be associated with a decreasing Eurasian snow cover in the simulations. An improvement in predicting the future climate change in regional scale is desirable.
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| 7. |
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| 8. |
- JEONG, SJ, et al.
(författare)
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Reduction of spring warming over East Asia associated with vegetation feedback
- 2009
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Ingår i: GEOPHYSICAL RESEARCH LETTERS. - 0094-8276. ; 36
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Tidskriftsartikel (refereegranskat)abstract
- Over East Asia, surface air temperature displays a significant increasing trend particularly in early months of the year for the period of 1982-2000. Warming per decade is strongest in late winter, 1.5 degrees C in February and 1.1 degrees C in March, but is significantly reduced in spring, 0.4 degrees C in April and 0.1 degrees C in May. During the analysis period, the reduced temperature increase from late winter to spring is found to be in contrast with the increased vegetation greenness derived from the satellite-measured leaf area index over the domain. We examined this inverse relationship using two climate model experiments-coupled with and without a dynamic vegetation model. In both experiments, strong warming in winter is relatively well reproduced, but weak warming in spring is observed only in the coupled experiment. Analysis of the surface energy budget indicates that weaker spring warming results from an evaporative cooling effect due to the increased vegetation greenness. Over East Asia, the vegetation-evaporation feedback, therefore, may produce seasonal asymmetry in the warming trend. Citation: Jeong, S.-J., C.-H. Ho, K.-Y. Kim, and J.-H. Jeong (2009), Reduction of spring warming over East Asia associated with vegetation feedback, Geophys. Res. Lett., 36, L18705, doi: 10.1029/2009GL039114.
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| 9. |
- Kim, B-M., et al.
(författare)
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Investigation of Stratospheric Precursor for the Cold Surge Event using Potential Vorticity Inversion Technique
- 2009
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Ingår i: Asia-Pacific Journal of Atmospheric Sciences. - 1976-7633. ; 45:4, s. 513-522
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Tidskriftsartikel (refereegranskat)abstract
- The downward influence of stratospheric potential vorticity (PV) on the cold surge occurrence over East Asia was examined. Strong negative PV anomalies in the upper stratosphere and the rising of geopotential height in the upper troposphere about one week ahead of the cold surge occurrence were suggested as precursory perturbations triggering the development of the cold surge, which is dynamically validated by the piecewise potential vorticity inversion technique. When the inversion is applied to the stratospheric PV anomalies, geopotential and temperature anomalies balanced to the stratospheric PV were remarkably well reproduced in both amplitude and penetrating depth as in the observations. Although the PV anomalies are mostly confined to the stratosphere, our results confirm that its downward influences are sufficiently strong such that associated tropospheric anomalies with the stratospheric PV are capable of triggering the cold surge occurrence within the given mechanism.
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| 10. |
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| 11. |
- Reddy, A. Satyanarayana, et al.
(författare)
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Ultrathin titania coating for high-temperature stable SiO(2)/Pt nanocatalysts
- 2011
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Ingår i: Chemical Communications. - : Royal Society of Chemistry (RSC). - 1359-7345 .- 1364-548X. ; 47, s. 8412-8414
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Tidskriftsartikel (refereegranskat)abstract
- The facile synthesis of silica supported platinum nanoparticles with ultrathin titania coating to enhance metal-support interactions suitable for high temperature reactions is reported, as thermal and structure stability of metal nanoparticles is important for catalytic reactions.
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| 12. |
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| 13. |
- Choi, Joong Il Jake, et al.
(författare)
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Surface Termination-Dependent Nanotribological Properties of Single-Crystal MAPbBr(3) Surfaces
- 2020
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Ingår i: The Journal of Physical Chemistry C. - : AMER CHEMICAL SOC. - 1932-7447 .- 1932-7455. ; 124:2, s. 1484-1491
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Tidskriftsartikel (refereegranskat)abstract
- Atomistic characterization of surface termination and the corresponding mechanical properties of single-crystal methylammonium lead tribromide (MAPbBr(3)) are performed using combined atomic force microscopy (AFM) measurements and density functional theory (DFT) calculations. A clean MAPbBr(3) surface is obtained by in situ cleavage in ultrahigh vacuum at room temperature, and the subsequent AFM measurements of the as-cleaved MAPbBr(3) exhibit the coexistence of two different surface terrace types with step height differences corresponding to about half the thickness of a PbI6 octahedron layer. Concurrent friction force microscopy measurements show that the two surfaces result in two distinct friction values. Based on DFT calculations, we attribute the higher-friction and lower-friction surfaces to MABr-terminated flat and PbBr2-terminated vacant surface terminations, respectively. The calculated electronic band structures of the various MABr- and PbBr2-terminated surfaces show that the midgap states are absent, revealing the defect-tolerant nature of the ideal single-crystal MAPbBr(3) surfaces.
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| 14. |
- HO, CH, et al.
(författare)
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Influence of stratospheric quasi-biennial oscillation on tropical cyclone tracks in the western North Pacific
- 2009
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Ingår i: GEOPHYSICAL RESEARCH LETTERS. - 0094-8276. ; 36
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Tidskriftsartikel (refereegranskat)abstract
- The possible influence of the stratospheric quasi-biennial oscillation (QBO) on tropical cyclone (TC) passages in the western North Pacific (WNP) is examined using TC data recorded by the Tokyo Typhoon Center and the QBO index derived from reanalysis data. The influence is observed to be significant. The number of TCs approaching the East China Sea is large during the westerly phase of the QBO; however, during the easterly phase, the number of TCs approaching the eastern offshore of Japan is large. This difference in the TC tracks is found to be related to the background flow change associated with the QBO. However, the total number of TC events over the WNP and the sum of the number of TCs approaching the aforementioned two regions appear to be unrelated to the QBO phases. Citation: Ho, C.-H., H.- S. Kim, J.- H. Jeong, and S.-W. Son (2009), Influence of stratospheric quasi-biennial oscillation on tropical cyclone tracks in the western North Pacific, Geophys. Res. Lett., 36, L06702, doi:10.1029/2009GL037163.
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| 15. |
- Jang, Jeong Keun, et al.
(författare)
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Area-efficient scheduling scheme based FFT processor for various OFDM systems
- 2018
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Ingår i: 2018 IEEE ASIA PACIFIC CONFERENCE ON CIRCUITS AND SYSTEMS (APCCAS 2018). - : IEEE. - 9781538682401 ; , s. 338-341
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- This paper presents an area-efficient fast Fouriertransform (FFT) processor for orthogonal frequency-division multiplexing systems based on multi-path delay commutator architecture. This paper proposes a data scheduling scheme to reduce the number of complex constant multipliers. The proposed mixed-radix multi-path delay commutator FFT processor can support 128-, 256-, and 512-point FFT sizes. The proposed processor was synthesized using the Samsung 65-nm CMOS standard cell library. The proposed processor with eight parallel data paths can achieve a high throughput rate of up to 2.64 GSample/s at 330 MHz.
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| 16. |
- Jeong, Jee-Hoon, 1976, et al.
(författare)
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Greening in the circumpolar high-latitude may amplify warming in the growing season
- 2012
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Ingår i: Climate Dynamics. - : Springer Science and Business Media LLC. - 0930-7575 .- 1432-0894. ; 38:7-8, s. 1421-1431
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Tidskriftsartikel (refereegranskat)abstract
- We present a study that suggests greening in the circumpolar high-latitude regions amplifies surface warming in the growing season (May–September) under enhanced greenhouse conditions. The investigation used a series of climate simulations with the Community Atmospheric Model version 3—which incorporates a coupled, dynamic global vegetation model—with and without vegetation feedback, under both present and doubled CO2 concentrations. Results indicate that climate warming and associated changes promote circumpolar greening with northward expansion and enhanced greenness of both the Arctic tundra and boreal forest regions. This leads to additional surface warming in the high-latitudes in the growing season, primarily through more absorption of incoming solar radiation. The resulting surface and tropospheric warming in the high-latitude and Arctic regions weakens prevailing tropospheric westerlies over 45–70N, leading to the formation of anticyclonic pressure anomalies in the Arctic regions. These pressure anomalies resemble the anomalous circulation pattern during the negative phase of winter Arctic Oscillation. It is suggested that these circulation anomalies reinforce the high-latitude and Arctic warming in the growing season.
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| 17. |
- Jeong, Jee-Hoon, 1976, et al.
(författare)
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Intensified Arctic warming under greenhouse warming by vegetation–atmosphere–sea ice interaction
- 2014
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Ingår i: Environmental Research Letters. - : IOP Publishing. - 1748-9326. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Observations and modeling studies indicate that enhanced vegetation activities over high latitudes under an elevated CO2 concentration accelerate surface warming by reducing the surface albedo. In this study, we suggest that vegetation-atmosphere-sea ice interactions over high latitudes can induce an additional amplification of Arctic warming. Our hypothesis is tested by a series of coupled vegetation-climate model simulations under 2xCO2 environments. The increased vegetation activities over high latitudes under a 2xCO2 condition induce additional surface warming and turbulent heat fluxes to the atmosphere, which are transported to the Arctic through the atmosphere. This causes additional sea-ice melting and upper-ocean warming during the warm season. As a consequence, the Arctic and high-latitude warming is greatly amplified in the following winter and spring, which further promotes vegetation activities the following year. We conclude that the vegetation-atmosphere-sea ice interaction gives rise to additional positive feedback of the Arctic amplification.
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| 18. |
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| 19. |
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| 20. |
- Kim, HoUng, et al.
(författare)
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The Future of Biosimilars : Maximizing Benefits Across Immune-Mediated Inflammatory Diseases
- 2020
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Ingår i: Drugs. - : Adis International. - 0012-6667 .- 1179-1950. ; 80:2, s. 99-113
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Tidskriftsartikel (refereegranskat)abstract
- Biologics have transformed the treatment of immune-mediated inflammatory diseases such as rheumatoid arthritis (RA) and inflammatory bowel disease (IBD). Biosimilars-biologic medicines with no clinically meaningful differences in safety or efficacy from licensed originators-can stimulate market competition and have the potential to expand patient access to biologics within the parameters of treatment recommendations. However, maximizing the benefits of biosimilars requires cooperation between multiple stakeholders. Regulators and developers should collaborate to ensure biosimilars reach patients rapidly without compromising stringent quality, safety, or efficacy standards. Pharmacoeconomic evaluations and payer policies should be updated following biosimilar market entry, minimizing the risk of imposing nonmedical barriers to biologic treatment. In RA, disparities between treatment guidelines and national reimbursement criteria could be addressed to ensure more uniform patient access to biologics and enable rheumatologists to effectively implement treat-to-target strategies. In IBD, the cost-effectiveness of biologic treatment earlier in the disease course is likely to improve when biosimilars are incorporated into pharmacoeconomic analyses. Patient understanding of biosimilars is crucial for treatment success and avoiding nocebo effects. Full understanding of biosimilars by physicians and carefully considered communication strategies can help support patients initiating or switching to biosimilars. Developers must operate efficiently to be sustainable, without undermining product quality, the reliability of the supply chain, or pharmacovigilance. Developers should also facilitate information sharing to meet the needs of other stakeholders. Such collaboration will help to ensure a sustainable future for both the biosimilar market and healthcare systems, supporting the availability of effective treatments for patients.
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| 21. |
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| 22. |
- Kim, Min-Seok, et al.
(författare)
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The Potential of Using Tree-Ring Chronology from the Southern Coast of Korea to Reconstruct the Climate of Subtropical Western North Pacific: A Pilot Study
- 2020
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Ingår i: Atmosphere. - : MDPI AG. - 2073-4433. ; 11
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Tidskriftsartikel (refereegranskat)abstract
- Tree-ring width (TRW) chronologies have successfully been used as climate proxies to infer climate variabilities over the past hundreds to thousands of years worldwide beyond observational records. However, these data are scarce over parts of subtropical East Asia, and especially over the Korean Peninsula. In this pilot study, Korean red pine (Pinus densiflora Siebold and Zucc.) TRW chronologies from Mt. Mudeung and Mt. Wolchul, South Korea, were developed, and their localto large-scale climatic responses were investigated. Mt. Mudeung TRWhad a positive association with local temperature in the preceding December and April. Mt. Wolchul TRW had a positive association with local temperature in the preceding December and most of the early summer to autumn months, and with local precipitation in February and October. On a large scale, both TRWs retained meaningful temperature and monsoon precipitation signals over East Asia and sea surface temperature signals over the Western North Pacific. The results suggest that the subtropical trees from South Korea can be used to infer past long-term climate variability at both local and large scales over East Asia and the Western North Pacific, such as the East Asian summer monsoon, the Kuroshio Current, theWestern North Pacific Subtropical High, and El Niño–Southern Oscillation.
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| 23. |
- Kim, Tae-Yun, et al.
(författare)
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3D Texture Analysis in Renal Cell Carcinoma Tissue Image Grading
- 2014
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Ingår i: Computational & Mathematical Methods in Medicine. - : Hindawi Publishing Corporation. - 1748-670X .- 1748-6718. ; 2014, s. 536217:1-12
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Tidskriftsartikel (refereegranskat)abstract
- One of the most significant processes in cancer cell and tissue image analysis is the efficient extraction of features for grading purposes. This research applied two types of three-dimensional texture analysis methods to the extraction of feature values from renal cell carcinoma tissue images, and then evaluated the validity of the methods statistically through grade classification. First, we used a confocal laser scanning microscope to obtain image slices of four grades of renal cell carcinoma, which were then reconstructed into 3D volumes. Next, we extracted quantitative values using a 3D gray level cooccurrence matrix (GLCM) and a 3D wavelet based on two types of basis functions. To evaluate their validity, we predefined 6 different statistical classifiers and applied these to the extracted feature sets. In the grade classification results, 3D Haar wavelet texture features combined with principal component analysis showed the best discrimination results. Classification using 3D wavelet texture features was significantly better than 3D GLCM, suggesting that the former has potential for use in a computer-based grading system.
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| 24. |
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| 25. |
- Lee, Sang Hoon, 1982-, et al.
(författare)
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Global organization of protein complexome in the yeast Saccharomyces cerevisiae
- 2011
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Ingår i: BMC Systems Biology. - : BioMed Central. - 1752-0509. ; 5:126, s. 15-
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Tidskriftsartikel (refereegranskat)abstract
- Background: Proteins in organisms, rather than act alone, usually form protein complexes to perform cellular functions. We analyze the topological network structure of protein complexes and their component proteins in the budding yeast in terms of the bipartite network and its projections, where the complexes and proteins are its two distinct components. Compared to conventional protein-protein interaction networks, the networks from the protein complexes show more homogeneous structures than those of the binary protein interactions, implying the formation of complexes that cause a relatively more uniform number of interaction partners. In addition, we suggest a new optimization method to determine the abundance and function of protein complexes, based on the information of their global organization. Estimating abundance and biological functions is of great importance for many researches, by providing a quantitative description of cell behaviors, instead of just a "catalogues" of the lists of protein interactions.Results: With our new optimization method, we present genome-wide assignments of abundance and biological functions for complexes, as well as previously unknown abundance and functions of proteins, which can provide significant information for further investigations in proteomics. It is strongly supported by a number of biologically relevant examples, such as the relationship between the cytoskeleton proteins and signal transduction and the metabolic enzyme Eno2's involvement in the cell division process.Conclusions: We believe that our methods and findings are applicable not only to the specific area of proteomics, but also to much broader areas of systems biology with the concept of optimization principle.
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| 26. |
- Lee, Sang Hoon, 1982-, et al.
(författare)
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Googling social interactions : web search engine based social network construction
- 2010
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Ingår i: PLoS ONE. - : Public Library of Science. - 1932-6203. ; 5:7, s. e11233-
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Tidskriftsartikel (refereegranskat)abstract
- Social network analysis has long been an untiring topic of sociology. However, until the era of information technology, the availability of data, mainly collected by the traditional method of personal survey, was highly limited and prevented large-scale analysis. Recently, the exploding amount of automatically generated data has completely changed the pattern of research. For instance, the enormous amount of data from so-called high-throughput biological experiments has introduced a systematic or network viewpoint to traditional biology. Then, is “high-throughput” sociological data generation possible? Google, which has become one of the most influential symbols of the new Internet paradigm within the last ten years, might provide torrents of data sources for such study in this (now and forthcoming) digital era. We investigate social networks between people by extracting information on the Web and introduce new tools of analysis of such networks in the context of statistical physics of complex systems or socio-physics. As a concrete and illustrative example, the members of the 109th United States Senate are analyzed and it is demonstrated that the methods of construction and analysis are applicable to various other weighted networks.
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| 27. |
- Lee, Sang Hoon, 1982-, et al.
(författare)
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Statistical properties of sampled networks
- 2006
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Ingår i: Physical Review E. Statistical, Nonlinear, and Soft Matter Physics. - 1063-651X .- 1095-3787. ; 73:016102
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Tidskriftsartikel (refereegranskat)abstract
- We study the statistical properties of the sampled scale-free networks, deeply related to the proper identification of various real-world networks. We exploit three methods of sampling and investigate the topological properties such as degree and betweenness centrality distribution, average path length, assortativity, and clustering coefficient of sampled networks compared with those of original networks. It is found that the quantities related to those properties in sampled networks appear to be estimated quite differently for each sampling method. We explain why such a biased estimation of quantities would emerge from the sampling procedure and give appropriate criteria for each sampling method to prevent the quantities from being overestimated or underestimated.
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| 28. |
- Park, Kwang-Hyun, et al.
(författare)
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RNA activation-independent DNA targeting of the Type III CRISPR-Cas system by a Csm complex
- 2017
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Ingår i: EMBO Reports. - : Wiley-VCH Verlagsgesellschaft. - 1469-221X .- 1469-3178. ; 18:5, s. 826-840
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Tidskriftsartikel (refereegranskat)abstract
- The CRISPR-Cas system is an adaptive and heritable immune response that destroys invading foreign nucleic acids. The effector complex of the Type III CRISPR-Cas system targets RNA and DNA in a transcription-coupled manner, but the exact mechanism of DNA targeting by this complex remains elusive. In this study, an effector Csm holocomplex derived from Thermococcus onnurineus is reconstituted with a minimalistic combination of Csm1(1)2(1)3(3)4(1)5(1), and shows RNA targeting and RNA-activated single-stranded DNA (ssDNA) targeting activities. Unexpectedly, in the absence of an RNA transcript, it cleaves ssDNA containing a sequence complementary to the bound crRNA guide region in a manner dependent on the HD domain of the Csm1 subunit. This nuclease activity is blocked by a repeat tag found in the host CRISPR loci. The specific cleavage of ssDNA without a target RNA suggests a novel ssDNA targeting mechanism of the Type III system, which could facilitate the efficient and complete degradation of foreign nucleic acids.
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| 29. |
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| 30. |
- Ugawa, Masashi, et al.
(författare)
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Reduced acoustic resonator dimensions improve focusing efficiency of bacteria and submicron particles
- 2022
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Ingår i: Analyst. - : Royal Society of Chemistry (RSC). - 1364-5528. ; 147:2, s. 274-281
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Tidskriftsartikel (refereegranskat)abstract
- In this study, we demonstrate an acoustofluidic device that enables single-file focusing of submicron particles and bacteria using a two-dimensional (2D) acoustic standing wave. The device consists of a 100 μm × 100 μm square channel that supports 2D particle focusing in the channel center at an actuation frequency of 7.39 MHz. This higher actuation frequency compared with conventional bulk acoustic systems enables radiation-force-dominant motion of submicron particles and overcomes the classical size limitation (≈2 μm) of acoustic focusing. We present acoustic radiation force-based focusing of particles with diameters less than 0.5 μm at a flow rate of 12 μL min−1, and 1.33 μm particles at flow rates up to 80 μL min−1. The device focused 0.25 μm particles by the 2D acoustic radiation force while undergoing a channel cross-section centered, single-vortex acoustic streaming. A suspension of bacteria was also investigated to evaluate the biological relevance of the device, which demonstrated the alignment of bacteria in the channel at aflow rate of up to 20 μL min−1. The developed acoustofluidic device can align submicron particles within a narrow flow stream in a highly robust manner, validating its use as a flow-through focusing chamber to perform high-throughput and accurate flow cytometry of submicron objects
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| 31. |
- Zhang, Peng, et al.
(författare)
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Abrupt shift to hotter and drier climate over inner East Asia beyond the tipping point
- 2020
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 370:6520, s. 1095-1099
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Tidskriftsartikel (refereegranskat)abstract
- Unprecedented heatwave-drought concurrences in the past two decades have been reported over inner East Asia. Tree-ring–based reconstructions of heatwaves and soil moisture for the past 260 years reveal an abrupt shift to hotter and drier climate over this region. Enhanced land-atmosphere coupling, associated with persistent soil moisture deficit, appears to intensify surface warming and anticyclonic circulation anomalies, fueling heatwaves that exacerbate soil drying. Our analysis demonstrates that the magnitude of the warm and dry anomalies compounding in the recent two decades is unprecedented over the quarter of a millennium, and this trend clearly exceeds the natural variability range. The “hockey stick”–like change warns that the warming and drying concurrence is potentially irreversible beyond a tipping point in the East Asian climate system.
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