| 1. |
- Ferreira, Daniel, et al.
(författare)
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The interactive effect of demographic and clinical factors on hippocampal volume : A multicohort study on 1958 cognitively normal individuals
- 2017
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Ingår i: Hippocampus. - : Wiley. - 1050-9631 .- 1098-1063. ; 27:6, s. 653-667
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Tidskriftsartikel (refereegranskat)abstract
- Alzheimer's disease is characterized by hippocampal atrophy. Other factors also influence the hippocampal volume, but their interactive effect has not been investigated before in cognitively healthy individuals. The aim of this study is to evaluate the interactive effect of key demographic and clinical factors on hippocampal volume, in contrast to previous studies frequently investigating these factors in a separate manner. Also, to investigate how comparable the control groups from ADNI, AIBL, and AddNeuroMed are with five population-based cohorts. In this study, 1958 participants were included (100 AddNeuroMed, 226 ADNI, 155 AIBL, 59 BRC, 295 GENIC, 279 BioFiNDER, 398 PIVUS, and 446 SNAC-K). ANOVA and random forest were used for testing between-cohort differences in demographic-clinical variables. Multiple regression was used to study the influence of demographic-clinical variables on hippocampal volume. ANCOVA was used to analyze whether between-cohort differences in demographic-clinical variables explained between-cohort differences in hippocampal volume. Age and global brain atrophy were the most important variables in explaining variability in hippocampal volume. These variables were not only important themselves but also in interaction with gender, education, MMSE, and total intracranial volume. AddNeuroMed, ADNI, and AIBL differed from the population-based cohorts in several demographic-clinical variables that had a significant effect on hippocampal volume. Variability in hippocampal volume in individuals with normal cognition is high. Differences that previously tended to be related to disease mechanisms could also be partly explained by demographic and clinical factors independent from the disease. Furthermore, cognitively normal individuals especially from ADNI and AIBL are not representative of the general population. These findings may have important implications for future research and clinical trials, translating imaging biomarkers to the general population, and validating current diagnostic criteria for Alzheimer's disease and predementia stages.
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| 2. |
- Moberg, Christina, et al.
(författare)
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De unga gör helt rätt när de stämmer staten
- 2022
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Ingår i: Aftonbladet. - 1103-9000.
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- 1 620 forskare och lärare i forskarvärlden: Vi ställer oss bakom Auroras klimatkrav
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| 3. |
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| 4. |
- Brechensbauer Brandin, Madeleine, et al.
(författare)
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Centralen : Studier i området kring Stockholms Centralstation
- 1989
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Rapport (populärvet., debatt m.m.)abstract
- Denna skrift handlar om Centralen i Stockholm och dess närmaste omgivningar, Centralplan, T-centralen, Vasagatan och Klarabergsgatan. Den är resultatet av Arkitekturskolans arbete läsåret 1983- 84 och innehåller förutom en rad projekt också historiska utblickar och samtidskritiska resonemang. En stor del av innehållet redovisades redan våren 1984 - det skedde genom en utställning på Arkitekturmuseet och en preliminär publikation. Materialet har sedan svällt ut med innehållsrika uppsatser om Centralen, om äldre och nyare insatser för att förena konst och arkitektur och om den föga kända, ännu obebyggda Blekholmen.
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| 10. |
- Ljungström, Lars, 1956-
(författare)
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Magnus Gabriel De la Gardies Venngarn : Herresätet som byggnadsverk och spegelbild
- 2004
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Starting in about 1640, Sweden experienced a building boom among the nobility lasting up to 1680, when Charles XI initiated a massive withdrawal of Crown fiefs. Based on contemporary Swedish sources, the strategies and ideology behind these large new houses are discussed in connection with questions concerning visual identity – corporate as well as individual. Opinions held in the Swedish debate are compared with the characteristics described by Volker Bauer in his analysis of competing German court ideal types, and are found strikingly reminiscent. This approach constitutes the framework for a close examination of Venngarn, one of Count Magnus Gabriel De la Gardie´s country houses, rebuilt and considerably enlarged from 1661 and onwards. De la Gardie was Rikskansler, Chancellor of the Realm, a dominating magnate and one of Sweden´s most eager building patrons. The remodelling of Venngarn was begun at a time when major works were in progress at five other estates, at the palace in Stockholm and his father´s burial chapel. Other projects were to follow suite. At Venngarn the idea of an ideal correspondence between the level of Magnificentia and the individual´s station is expressed by a systematic gradation of the apartments. Another court ideal, that of the prince (or aristocrat) as a disciple of the Muses, was just as befitting an attribute. The most telling example is the 'learned' décor of the chapel. More than fifty emblems, parables and devotional subjects are found to be organized according to a comprehensive programme, combining important tendencies in contemporary Lutheran spirituality, on the one hand themes from Johann Arndt, on the other the dogma of Ordo salutis. A more mundane aspect of aristocratic behaviour was villeggiatura, or the preference for temporary retreats from urban life and public affairs. De la Gardie has left several drafts defining the weekly round of a country life and aiming at an otium, as recommended by Seneca, Cicero and the neo-Stoics. Several circumstances indicate that De la Gardie considered this a component of a true statesman´s life and that Venngarn and his other country houses were to serve such a purpose.In these and other ways, De la Gardie could be said to have arranged the house at Venngarn as an ideal mirror image of his persona, an image that would testify to his greatness and further his reputation.
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| 11. |
- Rollborn, Niclas, et al.
(författare)
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Good Agreement Between Hba1c Analyzed Using Capillary Electrophoresis, HPLC, Immunological and Enzymatic Methods
- 2019
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Ingår i: Journal of Diabetes, Metabolism and its Complications. ; 1:1, s. 1-7
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: Hemoglobin A1c (HbA1c) is an essential marker for assessment of glycemic control in diabetes patients. The aim of this study was to evaluate the agreement between different HbA1c methods.Methodology: We used blood samples to compare HbA1c results analyzed with Capillarys 3 Tera, Roche Tina-Quant HbA1c Gen 3, BioRad Variant II Turbo (3 sites), Mono S® and Abbott Architect enzymatic method. The comparisons were made as paired instrument comparisons with Capillarys 3 Tera.Results: The linear correlations between the HbA1c methods were as follows:Cobas 6000 = 0.982 x Capillarys 3 Tera + 0.975, R² = 0.994;Architect c8000 = 0.982 x Capillarys 3 Tera + 1.064, R² = 0.994; Mono S® = 0.916 x Capillarys 3 Tera + 3.397, R² = 0.965;BioRad Variant II Turbo = 0.923 x Capillarys 3 Tera + 4.062, R² = 0.990; Tosoh G8 = 0.963 x Capillarys 3 Tera + 3.895, R² = 0.996.Conclusions: The different instrument platforms showed the best agreement in the 50-70 mmol/mol interval. Above and below this range the methods separated into 2 groups, one consisting of Capillarys 3 Tera, Roche Tina-Quant and Abbott enzymatic method and the other group consisting of BioRad Variant II Turbo, Tosoh G8 and Mono S®.
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| 12. |
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| 13. |
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| 14. |
- Sandström, Olof, et al.
(författare)
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Miljöpåverkan av skogsindustriutsläpp
- 1997
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Ingår i: Naturvårdsverkets rapportserie. Rapport 4695. ; , s. 1-89
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 15. |
- Sundström, Johan, Professor, 1971-, et al.
(författare)
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Risk factors for subarachnoid haemorrhage : a nationwide cohort of 950 000 adults
- 2019
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Ingår i: International Journal of Epidemiology. - : Oxford University Press. - 0300-5771 .- 1464-3685. ; 48:6, s. 2018-2025
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Subarachnoid haemorrhage (SAH) is a devastating disease, with high mortality rate and substantial disability among survivors. Its causes are poorly understood. We aimed to investigate risk factors for SAH using a novel nationwide cohort consortium.METHODS: We obtained individual participant data of 949 683 persons (330 334 women) between 25 and 90 years old, with no history of SAH at baseline, from 21 population-based cohorts. Outcomes were obtained from the Swedish Patient and Causes of Death Registries.RESULTS: During 13 704 959 person-years of follow-up, 2659 cases of first-ever fatal or non-fatal SAH occurred, with an age-standardized incidence rate of 9.0 [95% confidence interval (CI) (7.4-10.6)/100 000 person-years] in men and 13.8 [(11.4-16.2)/100 000 person-years] in women. The incidence rate increased exponentially with higher age. In multivariable-adjusted Poisson models, marked sex interactions for current smoking and body mass index (BMI) were observed. Current smoking conferred a rate ratio (RR) of 2.24 (95% CI 1.95-2.57) in women and 1.62 (1.47-1.79) in men. One standard deviation higher BMI was associated with an RR of 0.86 (0.81-0.92) in women and 1.02 (0.96-1.08) in men. Higher blood pressure and lower education level were also associated with higher risk of SAH.CONCLUSIONS: The risk of SAH is 45% higher in women than in men, with substantial sex differences in risk factor strengths. In particular, a markedly stronger adverse effect of smoking in women may motivate targeted public health initiatives.
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| 16. |
- Voevodskaya, Olga, et al.
(författare)
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The effects of intracranial volume adjustment approaches on multiple regional MRI volumes in healthy aging and Alzheimer's disease
- 2014
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Ingår i: Frontiers in Aging Neuroscience. - : Frontiers Media SA. - 1663-4365. ; 6, s. 264-
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Tidskriftsartikel (refereegranskat)abstract
- In neurodegeneration research, normalization of regional volumes by intracranial volume (ICV) is important to estimate the extent of disease-driven atrophy. There is little agreement as to whether raw volumes, volume-to-ICV fractions or regional volumes from which the ICV factor has been regressed out should be used for volumetric brain imaging studies. Using multiple regional cortical and subcortical volumetric measures generated by Freesurfer (51 in total), the main aim of this study was to elucidate the implications of these adjustment approaches. Magnetic resonance imaging (MRI) data were analyzed from two large cohorts, the population-based PIVUS cohort (N = 406, all subjects age 75) and the Alzheimer disease Neuroimaging Initiative (ADNI) cohort (N = 724). Further, we studied whether the chosen ICV normalization approach influenced the relationship between hippocampus and cognition in the three diagnostic groups of the ADNI cohort (Alzheimer's disease, mild cognitive impairment, and healthy individuals). The ability of raw vs. adjusted hippocampal volumes to predict diagnostic status was also assessed. In both cohorts raw volumes correlate positively with ICV, but do not scale directly proportionally with it. The correlation direction is reversed for all volume-to-ICV fractions, except the lateral and third ventricles. Most gray matter fractions are larger in females, while lateral ventricle fractions are greater in males. Residual correction effectively eliminated the correlation between the regional volumes and ICV and removed gender differences. The association between hippocampal volumes and cognition was not altered by ICV normalization. Comparing prediction of diagnostic status using the different approaches, small but significant differences were found. The choice of normalization approach should be carefully considered when designing a volumetric brain imaging study.
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| 17. |
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| 18. |
- Abrahamsson, Maria, et al.
(författare)
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A new strategy for the improvement of photophysical properties in ruthenium(II) polypyridyl complexes. Synthesis and photophysical and electrochemical characterization of six mononuclear ruthenium(II) bisterpyridine-type complexes
- 2005
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Ingår i: Inorganic Chemistry. - : American Chemical Society (ACS). - 0020-1669 .- 1520-510X. ; 44:9, s. 3215-3225
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Tidskriftsartikel (refereegranskat)abstract
- The synthesis and characterization of six ruthenium(II) bistridentate polypyridyl complexes is described. These were designed on the basis of a new approach to increase the excited-state lifetime of ruthenium(II) bisterpyridine-type complexes. By the use of a bipyridylpyridyl methane ligand in place of terpyridine, the coordination environment of the metal ion becomes nearly octahedral and the rate of deactivation via ligand-field (i.e., metal-centered) states was reduced as shown by temperature-dependent emission lifetime studies. Still, the possibility to make quasi-linear donor-ruthenium-acceptor triads is maintained in the complexes. The most promising complex shows an excited-state lifet me of tau = 15 ns in alcohol solutions at room temperature, which should be compared to a lifetime of tau = 0.25 ns for [Ru(tpy)(2)](2+). The X-ray structure of the new complex indeed shows a more octahedral geometry than that of [Ru(tpy)(2)](2+). Most importantly, the high excited-state energy was retained, and thus, so was the potential high reactivity of the excited complex, which has not been the case with previously published strategies based on bistridentate complexes.
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| 19. |
- Addinsall, Alex B., et al.
(författare)
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Ruxolitinib : A new hope for ventilator-induced diaphragm dysfunction
- 2024
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Ingår i: Acta Physiologica. - : John Wiley & Sons. - 1748-1708 .- 1748-1716. ; 240:5
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Tidskriftsartikel (refereegranskat)abstract
- Aim: Mechanical ventilation (MV) results in diminished diaphragm size and strength, termed ventilator-induced diaphragm dysfunction (VIDD). VID increases dependence, prolongs weaning, and increases discharge mortality rates. The Janus kinase (JAK)/Signal Transducer and Activator of Transcription (STAT) pathway is implicated in VIDD, upregulated following MV. JAK/STAT inhibition alleviates chronic muscle wasting conditions. This study aimed to explore the therapeutic potential of Ruxolitinib, an FDA approved JAK1/2 inhibitor (JI) for the treatment of VIDD. Methods: Rats were subjected to 5 days controlled MV (CMV) with and without daily Ruxolitinib gavage. Muscle fiber size and function were assessed. RNAseq, mitochondrial morphology, respirometry, and mass spectrometry were determined. Results: CMV significantly reduced diaphragm size and specific force by 45% (p < 0.01), associated with a two-fold P-STAT3 upregulation (p < 0.001). CMV disrupted mitochondrial content and reduced the oxygen consumption rate (p < 0.01). Expression of the motor protein myosin was unaffected, however CMV alters myosin function via post-translational modifications (PTMs). Daily administration of JI increased animal survival (40% vs. 87%; p < 0.05), restricted P-STAT3 (p < 0.001), and preserved diaphragm size and specific force. JI was associated with preserved mitochondrial content and respiratory function (p < 0.01), and the reversal or augmentation of myosin deamidation PTMs of the rod and head region. Conclusion: JI preserved diaphragm function, leading to increased survival in an experimental model of VIDD. Functional enhancement was associated with maintenance of mitochondrial content and respiration and the reversal of ventilator-induced PTMs of myosin. These results demonstrate the potential of repurposing Ruxolitinib for treatment of VIDD.
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| 20. |
- Akre [Fall], Katja, 1971-, et al.
(författare)
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Risk for gastric cancer after antibiotic prophylaxis in patients undergoing hip replacement
- 2000
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Ingår i: Cancer Research. - Birmingham, USA : American Asoociation for Cancer Research. - 0008-5472 .- 1538-7445. ; 60, s. 6376-
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Tidskriftsartikel (refereegranskat)abstract
- Despite strong evidence of an association between Helicobacter pylori and gastric cancer, the benefit of eradicating H. pylori infection is unknown. Our aim was to test the hypothesis that exposure to high doses of antibiotics reduces risk for gastric cancer via possible eradication of H. pylori We conducted a nationwide case-control study nested in a cohort of 39,154 patients who underwent hip replacement surgery between 1965 and 1983. Such patients frequently receive prophylactic antibiotic treatment. During follow-up through 1989, we identified 189 incident cases of gastric cancer. For each case, three controls were selected from the cohort. Exposure data were abstracted from hospital records. Blood samples from a separate cohort undergoing hip replacement surgery were analyzed for anti-H. pylori IgG before and after surgery. Both long-term antibiotic treatment before surgery [odds ratio (OR), 0.3; 95% confidence interval (CI), 0.1-0.7] and prophylactic antibiotic treatment (OR, 0.7; 95% CI, 0.5-1.1) conferred a reduction in gastric cancer risk. The reduction appeared stronger after 5 years (OR, 0.6; 95% CI, 0.3-1.2) than during shorter follow-up after hip replacement (OR, 0.8; 95% CI, 0.4-1.7). There was an apparent decrease in risk with increasing body weight-adjusted doses of antibiotics (P = 0.13). However, the rate of H. pylori antibody disappearance was not strikingly higher in the cohort of patients undergoing hip replacement than in a control cohort. Our findings provide indirect support for the hypothesis that treatment with antibiotics at a relatively advanced age reduces the risk of gastric cancer.
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| 21. |
- Alamdari, Nima, et al.
(författare)
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Loss of muscle strength during sepsis is in part regulated by glucocorticoids and is associated with reduced muscle fiber stiffness
- 2012
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Ingår i: American Journal of Physiology. Regulatory Integrative and Comparative Physiology. - : American Physiological Society. - 0363-6119 .- 1522-1490. ; 303:10, s. R1090-R1099
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Tidskriftsartikel (refereegranskat)abstract
- Sepsis is associated with impaired muscle function but the role of glucocorticoids in sepsis-induced muscle weakness is not known. We tested the role of glucocorticoids in sepsis-induced muscle weakness by treating septic rats with the glucocorticoid receptor antagonist RU38486. In addition, normal rats were treated with dexamethasone to further examine the role of glucocorticoids in the regulation of muscle strength. Sepsis was induced in rats by cecal ligation and puncture and muscle force generation (peak twitch and tetanic tension) was determined in lower extremity muscles. In other experiments, absolute and specific force as well as stiffness (reflecting the function of actomyosin cross-bridges) were determined in isolated skinned muscle fibers from control and septic rats. Sepsis and treatment with dexamethasone resulted in reduced maximal twitch and tetanic force in intact isolated extensor digitorum longus muscles. The absolute and specific maximal force in isolated muscle fibers was reduced during sepsis together with decreased fiber stiffness. These effects of sepsis were blunted (but not abolished) by RU38486. The results suggest that muscle weakness during sepsis is at least in part regulated by glucocorticoids and reflects loss of contractility at the cellular (individual muscle fiber) level. In addition, the results suggest that reduced function of the cross-bridges between actin and myosin (documented as reduced muscle fiber stiffness) may be involved in sepsis-induced muscle weakness. An increased understanding of mechanisms involved in loss of muscle strength will be important for the development of new treatment strategies in patients with this debilitating consequence of sepsis.
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| 22. |
- Alrutz, Marie, et al.
(författare)
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Projektledning
- 2013
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Bok (populärvet., debatt m.m.)abstract
- Projektledning är ett yrke med egen certifiering. Det pågår en spännande utveckling inom området och det blir allt viktigare att hålla sig ajour med utvecklingen.Det övergripande målet med den här handboken är att vara en ständigt aktuell heltäckande bok om projektarbete. Innehållsmässigt täcker den både frågor som har med struktur och styrning att göra och frågor om ledning av människor och mänskliga processer i grupp. Kompetens inom projekt byggs av både kunskap och erfarenhet. Vi följer kontinuerligt aktuell forskning inom dessa områden och bjuder in intressanta forskare att medverka som författare. Vi skildrar verkliga projekt och låter erfarenheterna få plats, både de bästa erfarenheterna och de utmaningar som man tagit sig igenom.Handboken är levande och det innebär att artiklar tas bort för att ge plats för nya, i takt med att den uppdateras fortlöpande.
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| 23. |
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| 24. |
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| 26. |
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| 27. |
- Bergenstråhle-Wohlert, Malin, et al.
(författare)
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Concentration enrichment of urea at cellulose surfaces : results from molecular dynamics simulations and NMR spectroscopy
- 2012
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Ingår i: Cellulose. - : Springer Science and Business Media LLC. - 0969-0239 .- 1572-882X. ; 19:1, s. 1-12
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Tidskriftsartikel (refereegranskat)abstract
- A combined solid-state NMR and Molecular Dynamics simulation study of cellulose in urea aqueous solution and in pure water was conducted. It was found that the local concentration of urea is significantly enhanced at the cellulose/solution interface. There, urea molecules interact directly with the cellulose through both hydrogen bonds and favorable dispersion interactions, which seem to be the driving force behind the aggregation. The CP/MAS (13)C spectra was affected by the presence of urea at high concentrations, most notably the signal at 83.4 ppm, which has previously been assigned to C4 atoms in cellulose chains located at surfaces parallel to the (110) crystallographic plane of the cellulose I beta crystal. Also dynamic properties of the cellulose surfaces, probed by spin-lattice relaxation time (13)CT (1) measurements of C4 atoms, are affected by the addition of urea. Molecular Dynamics simulations reproduce the trends of the T (1) measurements and lends new support to the assignment of signals from individual surfaces. That urea in solution is interacting directly with cellulose may have implications on our understanding of the mechanisms behind cellulose dissolution in alkali/urea aqueous solutions.
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| 28. |
- Bergman, Karl-Olof, et al.
(författare)
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Clear-cuts are temporary habitats, not matrix, for endangered grassland burnet moths (Zygaena spp.)
- 2020
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Ingår i: Journal of Insect Conservation. - : Springer Science and Business Media LLC. - 1366-638X .- 1572-9753. ; 24:2, s. 269-277
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Tidskriftsartikel (refereegranskat)abstract
- Burnet moths (Zygaena spp.) are day-flying Lepidoptera considered indicative of species-rich grasslands. In the present study, our aim was to clarify whether clear-cuts are habitat, supporting habitat or matrix for three species of Zygaena. We did so by sampling these species with sex pheromones on 48 clear-cuts, varying in amount of host and nectar plants, in southern Sweden. To compare the efficiency of such sampling, we also conducted transect walks on these clearcuts. Overall, host-plants on clear-cuts best explained the abundance of Zygaena spp. recorded, better than nectar-plants or connectivity with nearby grasslands. These results indicate that clear-cuts with an abundance of host plants are used as a fully functional habitat, and not a supporting habitat in the sense of only providing nectar. There is no support in these results for considering clear-cuts as an inert matrix. With about half the work-effort, pheromone traps recorded 100 times more Zygaena spp. as transect walks. The poor correspondence between observations during transects walks and pheromone trap catches suggest Zygaena spp. being difficult to monitor by transect walks. In contrast to grasslands, clear-cuts are short-term in nature requiring repeated recolonization, indicating the importance of permanent grasslands. However, clear-cuts are important temporary insect habitats due to their great acreage, and suitable management can increase the time they remain a habitat.
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| 29. |
- Björk, Jonas, et al.
(författare)
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Accuracy of GFR estimating equations combining standardized cystatin C and creatinine assays: a cross-sectional study in Sweden
- 2015
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Ingår i: Clinical Chemistry and Laboratory Medicine. - : Walter de Gruyter GmbH. - 1434-6621 .- 1437-4331. ; 53:3, s. 403-414
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Tidskriftsartikel (refereegranskat)abstract
- Background: The recently established international cystatin C calibrator makes it possible to develop non-laboratory specific glomerular filtration rate (GFR) estimating (eGFR) equations. This study compares the performance of the arithmetic mean of the revised Lund-Malmo creatinine and CAPA cystatin C equations (MEAN(LM-REV+CAPA)), the arithmetic mean of the Chronic Kidney Disease Epidemiology Collaboration equation (CKD-EPI) creatinine and cystatin C equations (MEAN(CKD-EPI)), and the composite CKD-EPI equation (CKD-EPICREA+CYSC) with the corresponding single marker equations using internationally standardized calibrators for both cystatin C and creatinine. Methods: The study included 1200 examinations in 1112 adult Swedish patients referred for measurement of GFR (mGFR) 2008-2010 by plasma clearance of iohexol (median 51 mL/min/1.73 m(2)). Bias, precision (interquartile range, IQR) and accuracy (percentage of estimates +/- 30% of mGFR; P-30) were compared. Results: Combined marker equations were unbiased and had higher precision and accuracy than single marker equations. Overall results of MEAN(LM-REV+CAPA)/MEAN(CKD-EPI)/CKD-EPICREA+CYSC were: median bias -2.2%/-0.5%/-1.6%, IQR 9.2/9.2/8.8 mL/min/1.73 m(2), and P-30 91.3%/91.0%/91.1%. The P-30 figures were about 7-14 -percentage points higher than the single marker equations. The combined equations also had a more stable performance across mGFR, age and BMI intervals, generally with P-30 >= 90% and never <80%. Combined equations reached P-30 of 95% when the difference between eGFR(CREA) and eGFR(CYSC) was <10% but decreased to 82% at a difference of >= 40%. Conclusions: Combining cystatin C and creatinine assays improves GFR estimations with P-30 >= 90% in adults. Reporting estimates of both single and combined marker equations in clinical settings makes it possible to assess the validity of the combined equation based on the agreement between the single marker equations.
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| 30. |
- Borrelli, Pablo, et al.
(författare)
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Artificial intelligence-based detection of lymph node metastases by PET/CT predicts prostate cancer-specific survival
- 2021
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Ingår i: Clinical Physiology and Functional Imaging. - : Wiley. - 1475-0961 .- 1475-097X. ; 41:1, s. 62-67
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Tidskriftsartikel (refereegranskat)abstract
- Introduction Lymph node metastases are a key prognostic factor in prostate cancer (PCa), but detecting lymph node lesions from PET/CT images is a subjective process resulting in inter-reader variability. Artificial intelligence (AI)-based methods can provide an objective image analysis. We aimed at developing and validating an AI-based tool for detection of lymph node lesions. Methods A group of 399 patients with biopsy-proven PCa who had undergone(18)F-choline PET/CT for staging prior to treatment were used to train (n = 319) and test (n = 80) the AI-based tool. The tool consisted of convolutional neural networks using complete PET/CT scans as inputs. In the test set, the AI-based lymph node detections were compared to those of two independent readers. The association with PCa-specific survival was investigated. Results The AI-based tool detected more lymph node lesions than Reader B (98 vs. 87/117;p = .045) using Reader A as reference. AI-based tool and Reader A showed similar performance (90 vs. 87/111;p = .63) using Reader B as reference. The number of lymph node lesions detected by the AI-based tool, PSA, and curative treatment was significantly associated with PCa-specific survival. Conclusion This study shows the feasibility of using an AI-based tool for automated and objective interpretation of PET/CT images that can provide assessments of lymph node lesions comparable with that of experienced readers and prognostic information in PCa patients.
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| 31. |
- Burman, Joseph, et al.
(författare)
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Revealing hidden species distribution with pheromones: the caseof Synanthedon vespiformis (Lepidoptera: Sesiidae) in Sweden
- 2016
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Ingår i: Journal of Insect Conservation. - : Springer Science and Business Media LLC. - 1366-638X .- 1572-9753. ; 20:1, s. 11-21
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Tidskriftsartikel (refereegranskat)abstract
- Synanthedon vespiformis L. (Lepidoptera:Sesiidae) is considered a rare insect in Sweden, discoveredin 1860, with only a few observations recorded until a sexpheromone attractant became available recently. This studydetails a national survey conducted using pheromones as asampling method for this species. Through pheromonetrapping we captured 439 specimens in Southern Sweden at77 sites, almost tripling the number of previously reportedrecords for this species. The results suggest that S. vespiformisis truly a rare species with a genuinely scattereddistribution, but can be locally abundant. Habitat analyseswere conducted in order to test the relationship betweenhabitat quality and the number of individuals caught. InSweden, S. vespiformis is thought to be associated with oakhosts, but our attempts to predict its occurrence by theabundance of oaks yielded no significant relationships. Wetherefore suggest that sampling bias and limited knowledgeon distribution may have led to the assumption that thisspecies is primarily reliant on oaks in the northern part ofits range, whereas it may in fact be polyphagous, similar toS. vespiformis found as an agricultural pest in Central andSouthern Europe. We conclude that pheromones canmassively enhance sampling potential for this and otherrare lepidopteran species. Large-scale pheromone-basedsurveys provide a snapshot of true presences and absencesacross a considerable part of a species national distributionrange, and thus for the first time provide a viable means ofsystematically assessing changes in distribution over timewith high spatiotemporal resolution.
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| 32. |
- Dahlback, M, et al.
(författare)
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Enhanced insulin absorption in the rabbit airways and lung by sodium dioctyl sulfosuccinate
- 2002
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Ingår i: Journal of Aerosol Medicine. - : Mary Ann Liebert Inc. - 0894-2684 .- 1557-9026. ; 15:1, s. 27-36
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Tidskriftsartikel (refereegranskat)abstract
- The objective of this investigation was to study regional absorption of inhaled insulin together with an enhancer (sodium di-octyl-sulfosuccinate [DOSS]) in the rabbit airways and lung. Insulin was administered with or without DOSS by aerosol inhalation, intratracheal infusion, intranasally, sublingually, and without DOSS intravenously. Blood glucose and plasma levels of insulin were measured during 100 min from the start of administration. Inhalation of insulin (3 U) with 0.25% or 1% DOSS decreased average blood glucose levels significantly more than inhalation of insulin (3 U) without DOSS. Intratracheal administration of 1.5 U of insulin with 0.25% DOSS in 0.3 mL of vehicle decreased the average blood glucose level significantly compared with intratracheal administration of 1.5 U of insulin and no DOSS in 0.3 mL of vehicle and compared with 1.5 U of insulin with 0.25% DOSS in 0.15 mL of vehicle. Intravenous insulin (1.5 U) and inhaled (1.5 U) insulin in 0.25% DOSS decreased average blood glucose levels significantly compared with intratracheal (0.15 mL), intranasal, and sublingual administration of 1.5 U of insulin with 0.25% DOSS. The bioavailability of inhaled insulin (1.5 U) with 0.25% DOSS was estimated to be 16% in comparison with 7% for intratracheally (0.15 mL), 1% intranasally, and 0.8% sublingually administered insulin (1.5 U with 0.25% DOSS), respectively. Inhaled insulin together with the absorption enhancer DOSS decreased the blood glucose level more effectively than insulin given intratracheally, intranasally, or sublingually. The effect on blood glucose reflected the difference in plasma insulin concentration for the different routes of administration.
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| 33. |
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| 34. |
- DAWODY, JAZAER, 1959, et al.
(författare)
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An integrated system for energy-efficient exhaust aftertreatment for heavy-duty vehicles
- 2015
-
Ingår i: Renewable Energy in the Service of Mankind. - Cham : Springer International Publishing. - 9783319177779 - 9783319177762 ; 1, s. 133-143
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- © Springer International Publishing Switzerland 2015. This chapter presents a unique system approach applied in a joint academic- industrial research programme, E4 Mistra, to attain the goals of high energy efficiency and low emissions in an exhaust aftertreatment system for heavy-duty vehicles. The high energy efficiency is achieved by heat recuperation, onboard hydrogen production for NOx reduction, and by finding new solutions for making the aftertreatment system active at low exhaust temperatures. To reach low particulate emissions, a mechanical filter using a sintered metal powder is developed and coated with catalytic material to improve the soot oxidation efficiency. Low NOx emissions are achieved by an efficient NOx reduction catalyst. The integrated E4 Mistra system comprises four technological advances: thermoelectric (TE) materials for heat recuperation, catalytic reduction of NOx over innovative catalyst substrates using either the onboard diesel or biodiesel, H2 from a high-efficiency fuel reformer, and particulate filtration over a porous metal filter. The TE materials are used in a TE generator (TEG) which converts thermal energy into electricity. The TEG is used to recuperate heat from the exhaust-gas recirculation (EGR) circuit of heavy-duty trucks and is expected to generate ~1 kW electric power from 20 kW heat in the exhaust gas. The TEG is integrated in a plate heat exchanger (HEX) designed particularly for this application. Apart from the knowledge and experiences in TEG and heat exchange technologies, a thorough fluid dynamics and TE analysis are performed in this project to understand the governing processes and optimize the system accordingly. The components of the E4 Mistra system are explained in the chapter in addition to test results, which show the system's capacity for H2 production, NOx conversion, particulate matter filtration and soot oxidation, and finally electric power generation via heat recuperation from the exhaust gas using the developed TEG-HEX system.
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| 35. |
- Delsing, Lars-Olof, et al.
(författare)
-
From ’big’ to ’much’ : On the grammaticalisation of some gradable adjectives in Swedish
- 2021
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Ingår i: Morphosyntactic Change in Late Modern Swedish. - 9783961103256 - 9783985540211 ; 2, s. 319-337
-
Bokkapitel (refereegranskat)abstract
- In this paper, I give a short description of a language change that can be viewedas an instance of grammaticalization, namely the transition of the two adjectivesmycken/t and liten/t into quantifiers. Data from the corpus of Swedish drama dialogue reveal that liten/t became a quantifier as early as the 1700s, whereas mycken/t seems to have gone through the same change roughly 150 years later. Inherent plurals (such as pengar, ‘money’) appear to be a promising context for the starting point of the transition. I further illustrate how both quantifiers have weak and strong versions in present-day Swedish, and I argue that weak mycket (myke) has turned into a negative polarity item that is found in negated clauses (but not for example in questions and conditionals), whereas weak lite(t) has turned into a positive polarity item and is found elsewhere. If we assume that weak versions of quantifiers are more frequent than strong ones, and that positive polarity contexts are more frequent than negative ones, we expect the frequency of mycken/t to drop, whereas the frequency of liten/t should rise over time. A preliminary study that seems to confirm this prediction is presented here.
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| 36. |
- Edoff, Marika, 1965-, et al.
(författare)
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Post Deposition Treatments of (Ag,Cu)(In,Ga)Se-2 Thin Films for Solar Cells
- 2019
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Ingår i: 2019 IEEE 46th Photovoltaic Specialists Conference (PVSC). - New York : IEEE. - 9781728104942 ; , s. 618-621
-
Konferensbidrag (refereegranskat)abstract
- Different alkali alternatives for post-deposition of ACIGS were tested, both conventional fluoride salts and in the form of metals. XPS analysis of surfaces treated with K or KF as well as Rb or RbF have been performed, before (only for K and Rb) and after an ammonia etch. In addition to a strong suppression of Cu and Ag near the surface, we observe a difference in the re-distribution of Ga in the surface region after the etch depending on pdt element. Our results are consistent with the formation of K-In-Se and Rb-In-Se compounds for both metal alkalis and alkali fluorides. We find a similar beneficial effect on cell performance for the best cells with the metals as with the fluoride salts.
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| 37. |
- Ek, Helene, et al.
(författare)
-
Integrated fish monitoring in Sweden
- 2009
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The purpose of this report is to present a review and an evaluation of thestrategy of integrated fish monitoring included in the Swedish national marinemonitoring programme. As a background to the review an assessment is madeof some of the long-term changes observed in monitoring.The project is financed by the Environment Protection Agency (Contract no.212 0326) and the Faculty of Science, Göteborg University. The project leader isProf. Åke Larsson. Dr Olof Sandström, Skärgårdsutveckling SKUTAB AB, is contractedas external reviewer of the programme. Subproject leaders and participantsin the programme have contributed with results and comments on thepaper.
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| 38. |
- Ekblad, Tobias, 1979-, et al.
(författare)
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Patterned Hydrogels for Controlled Platelet Adhesion from Whole Blood and Plasma
- 2010
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Ingår i: Advanced Functional Materials. - : John Wiley & Sons. - 1616-301X .- 1616-3028. ; 20:15, s. 2396-2403
-
Tidskriftsartikel (refereegranskat)abstract
- This work describes the preparation and properties of hydrogel surface chemistries enabling controlled and well-defined cell adhesion. The hydrogels may be prepared directly on plastic substrates, such as polystyrene slides or dishes, using a quick and experimentally simple photopolymerization process, compatible with photolithographic and microfluidic patterning methods. The intended application for these materials is as substrates for diagnostic cell adhesion assays, particularly for the analysis of human platelet function. The adsorption of fibrinogen and other platelet promoting molecules is shown to be completely inhibited by the hydrogel, provided that the film thickness is sufficient (>5 nm). This allows the hydrogel to be used as a matrix for presenting selected bioactive ligands without risking interference from nonspecifically adsorbed platelet adhesion factors, even in undiluted whole blood and blood plasma. This concept is demonstrated by preparing patterns of proteins on hydrogel surfaces, resulting in highly controlled platelet adhesion. Further insights into the protein immobilization and platelet adhesion processes are provided by studies using imaging surface plasmon resonance. The hydrogel surfaces used in this work appear to provide an ideal platform for cell adhesion studies of platelets, and potentially also for other cell types.
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| 39. |
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| 40. |
- Eng Larsson, Fredrik, et al.
(författare)
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Explaining the cyclic behavior of freight transport CO2-emissions in Sweden over time
- 2012
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Ingår i: Transport Policy. - : Elsevier BV. - 1879-310X .- 0967-070X. ; 23, s. 79-87
-
Tidskriftsartikel (refereegranskat)abstract
- Economic growth is often considered to be the main factor behind, and tightly coupled to, the increase in freight transport work and its energy use. Recent research has quantified the relative contribution from underlying factors like value density of products, transport intensity and carbon intensity of fuel. In this work we rely on the theory of economic growth cycles in order to explain the dynamic behavior of some of these indicators. Focusing on the current growth cycle, we analyze Swedish data in a Shapley decomposition model, and the behaviors of the underlying factors are confronted against the growth cycle theory and recent findings in micro logistics. Our results suggests that the different and changing relations between growth and emission over the growth cycle indicate that the observed development in emissions is far from linear and cannot be explained straightforwardly by economic growth. The impact of the respective factor, and the relation between them, changes over time and results in different degrees of decoupling. The general trend is that micro-oriented factors tend to be more important in the rationalization period while macro-oriented factors have a stronger impact during the transformation period. We suggest that our approach might be useful not only for analyzing historical data, but also for medium-term and long-term scenarios for freight transport development and CO2- emissions. (c) 2012 Elsevier Ltd. All rights reserved.
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| 41. |
- Engstedt, Olof, et al.
(författare)
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Assessment of natal origin of pike (Esox lucius) in the Baltic Sea using Sr:Ca in otoliths
- 2010
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Ingår i: Environmental Biology Of Fishes. - : Springer Science and Business Media LLC. - 0378-1909 .- 1573-5133. ; 89:3-4, s. 547-555
-
Konferensbidrag (refereegranskat)abstract
- Spawning habitat of pike (Esox lucius) in the Baltic Sea include brackish water bays, brooks and rivers. Elevated salinity concentrations are one of several stressors that might increase the use and importance of freshwater habitats for spawning. In the Baltic Sea, one of the largest brackish seas in the world, freshwater species like pike, perch (Perca fluviatilis), whitefish (Coregonus sp), bream (Abramis brama), ide (Leuciscus idus), roach (Rutilus rutilus) and burbot (Lola iota) all undertake spawning migrations to freshwater. However, over the last decades populations densities of these species have declined, and recruitment failure has been argued to be at least part of the problem. The importance of brooks and rivers as spawning areas for these species have not been quantified and set in relation to spawning success in brackish bays. In this study, we collected 175 adult pike (Esox lucius) on their foraging grounds in the sea. Fish were collected in two regions on the Baltic coast, more than 600 km apart. Subsequently we determined their origin (freshwater or marine) using otolith chemistry. Sagittal otoliths were analysed for strontium using the PIXE-method. The results show that 80 of the 175 pike were recruited in freshwater, and several of the larger specimens showed reoccurring migration behaviour. Data show that freshwater is an important recruitment habitat for Baltic Sea pike, suggesting that habitat improvements in rivers entering the Baltic Sea might significantly contribute to population restoration.
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| 42. |
- Engström, Carl-Peter, 1945, et al.
(författare)
-
Functional status and well being in chronic obstructive pulmonary disease with regard to clinical parameters and smoking: a descriptive and comparative study.
- 1996
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Ingår i: Thorax. - 0040-6376. ; 51:8, s. 825-30
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Self-assessment questionnaires which measure the functional and affective consequences of chronic obstructive pulmonary disease (COPD) give valuable information about the effects of the disease and may serve as important tools with which to evaluate treatment. METHODS: A cross sectional comparative study was performed between patients with COPD (n = 68), stratified according to pulmonary function, and a healthy control group (n = 89). A battery of well established clinical and quality of life measures (the Sickness Impact Profile (SIP), Mood Adjective Check List (MACL), and Hospital Anxiety and Depression scale (HAD)) was used to examine in which functional and affective aspects the patient group differed from the control group and how these measures related to pulmonary function and smoking habits. RESULTS: Compared with the controls, COPD affected functional status in most areas, not just those requiring physical activity. Forty six patients with forced expiratory volume in one second (FEV1) below 50% predicted showed particularly high levels of dysfunction in ambulation, eating, home management, and recreation/ pastimes (SIP). Despite this, their level of psychosocial functioning and mood status was little different from that of the healthy controls. Among the patients, a subgroup reported substantial psychological distress, but mood status was only weakly, or not at all, related to pulmonary function. Smoking habits did not affect functional status or well being. CONCLUSIONS: Quality of life is not significantly affected in patients with mild to moderate loss of pulmonary function, possibly due to coping and/or pulmonary reserve capacity. This suggests that generic self-assessment questionnaires are of limited value for detecting the early consequences of COPD. However, in later stages of the disease they are sensitive enough to discriminate between patients with different levels of pulmonary dysfunction. The low correlations between the indices of pulmonary function and the indices of affective status suggest that well being depends, to a large extent, on factors outside the clinical domain.
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| 43. |
- Engström, Carl-Peter, 1945, et al.
(författare)
-
Health-related quality of life in COPD: why both disease-specific and generic measures should be used.
- 2001
-
Ingår i: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology. - 0903-1936. ; 18:1, s. 69-76
-
Tidskriftsartikel (refereegranskat)abstract
- Although research has consistently demonstrated that chronic obstructive pulmonary disease (COPD) impairs health-related quality of life (HRQL), little agreement has been evidenced regarding the factors identified as contributing to impaired HRQL. The aim was to study such factors using well established generic and specific HRQL instruments. The patients (n=68) were stratified by forced expiratory volume in one second (FEV1) to represent a wide range of disease severity. Pulmonary function, blood gases and 6-min walking distance test (6MWD) were assessed. HRQL instruments included: St George's Respiratory Questionnaire (SGRQ), Sickness Impact Profile (SIP), Hospital Anxiety and Depression Scale and Mood Adjective Check List. The strength of the impact of COPD on HRQL was represented along a continuum ranging from lung function, functional status (physical and psychosocial) to wellbeing. Although correlations between FEV1 versus SGRQ total and SIP overall scores (r=-0.42 and -0.32) were stronger than previously reported, multiple regression analyses showed that lung function contributed little to the variance when dyspnoea-related limitation, depression scores and 6MWD were included in the models. These three factors were important to varying degrees along the whole range of HRQL. Physiological, functional and psychosocial consequences of chronic obstructive pulmonary disease are only poorly to moderately related to each other. The present study concludes that a comprehensive assessment of the effects of chronic obstructive pulmonary disease requires a battery of instruments that not only tap the disease-specific effects, but also the overall burden of the disease on everyday functioning and emotional wellbeing.
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| 44. |
- Engström, Carl-Peter, 1945, et al.
(författare)
-
Long-term effects of a pulmonary rehabilitation programme in outpatients with chronic obstructive pulmonary disease: a randomized controlled study.
- 1999
-
Ingår i: Scandinavian journal of rehabilitation medicine. - 0036-5505. ; 31:4, s. 207-13
-
Tidskriftsartikel (refereegranskat)abstract
- Fifty patients with severe chronic obstructive pulmonary disease (FEV1 < 50% pred.) were randomized to a rehabilitation group and a control group. The rehabilitation group took part in an individualized multidisciplinary, outpatient 12-month rehabilitation programme. Exercise training was intensive during the first 6 weeks and was then gradually replaced by an individual home-training programme and booster sessions. Controls received the usual outpatient care. Positive effects were found in terms of maximum symptom-limited exercise tolerance and walking distance (13.5 and 12.1% increase, respectively) in the rehabilitation group compared with the controls. Quality of life measurements showed minor beneficial effects on the Sickness Impact Profile, indicating a higher level of activity. No effect was seen on the St George's Respiratory Questionnaire or the Mood Adjective Check List. Patients expressed their enthusiasm for the rehabilitation programme in a study-specific questionnaire.
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| 45. |
- Engström, Carl-Peter, 1945, et al.
(författare)
-
Reliability and validity of a Swedish version of the St George's Respiratory Questionnaire.
- 1998
-
Ingår i: The European respiratory journal : official journal of the European Society for Clinical Respiratory Physiology. - 0903-1936. ; 11:1, s. 61-6
-
Tidskriftsartikel (refereegranskat)abstract
- The St George's Respiratory Questionnaire (SGRQ) was designed to measure quality of life (QoL) in obstructive pulmonary disease. Its reliability, validity and sensitivity have been demonstrated. The aim was to develop a Swedish version of the SGRQ and to confirm its scaling and clinical properties. The SGRQ was adapted for Swedish conditions following a translation-backtranslation procedure. The psychometric and clinical evaluation included 68 patients with chronic obstructive pulmonary disease (COPD). Supplementary QoL, clinical and physiological data were collected. A follow-up study was performed 1 yr later. Correlation analysis used a multitrait-multimethod model. Internal consistency reliability and discriminant validity were documented by performing a multitrait analysis. The results confirmed expected levels of associations. Correlation coefficients between the SGRQ total score and the Sickness Impact Profile Total score (a generic health measure), forced expiratory volume in one second (FEV1) and 6 min walking distance were 0.69, -0.42 and -0.61 respectively. The pattern of correlations in the Swedish data set was very similar to that of the original. The stability of the SGRQ scores was confirmed at follow-up after 1 yr. The reliability was satisfactory, with Cronbach's alpha coefficients >0.80 for the SGRQ and its subdimensions. In conclusion, the Swedish version of the St George's Respiratory Questionnaire is reliable, valid and compares well with the corresponding tests of the original version.
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| 46. |
- Fjällbrant, Harald, 1961, et al.
(författare)
-
BCG scar and tuberculin reactivity in children and adults.
- 2008
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Ingår i: Scandinavian journal of infectious diseases. - : Informa UK Limited. - 0036-5548 .- 1651-1980. ; 40:5, s. 387-92
-
Tidskriftsartikel (refereegranskat)abstract
- Bacille Calmette-Guérin (BCG) vaccination generally leads to scar formation and tuberculin skin test (TST) reactivity. This study aimed at analysing these 2 parameters and their correlation in a setting with a low prevalence of tuberculosis. Retrospectively, we analysed 314 children and 390 adults living in Sweden and known from records or individual recall to have undergone BCG vaccination. A BCG scar was present in 161 (51%) of the children and in 340 (87%) of the adults. Among children with a scar, 94 (58%) were TST-positive (>/=6 mm) compared to 23 (15%) of 154 children lacking a visible scar. Among adults with a scar, 258 (76%) were TST- positive compared to 23 (46%) of 50 with no scar. Out of 152 non-vaccinated adults, 142 (94.4%) were TST-negative. When 175 TST-negative health care students were BCG-vaccinated in a prospective part of the study, 174 (99%) were found to develop a scar. In essence, the study showed a positive correlation between scar presence and TST reactivity. Furthermore, BCG vaccination of adults in the present setting resulted in consistent scar formation, while scar prevalence in previously vaccinated children was low.
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| 47. |
- Fjällbrant, Harald, 1961, et al.
(författare)
-
Primary vaccination and revaccination of young adults with BCG: a study using immunological markers.
- 2007
-
Ingår i: Scandinavian journal of infectious diseases. - : Informa UK Limited. - 0036-5548 .- 1651-1980. ; 39:9, s. 792-8
-
Tidskriftsartikel (refereegranskat)abstract
- Questions have been raised about the effectiveness of Bacille Calmette-Guérin (BCG) vaccination against tuberculosis (TB) in adults. We therefore analysed the immune response after BCG vaccination in primary-vaccinated and revaccinated young adults. 31 tuberculin skin test (TST) negative healthy students were BCG-vaccinated; 15 were primary-vaccinated and 16 revaccinated. Tuberculin-induced lymphocyte transformation (LT) and cytokine production of peripheral blood mononuclear cells were studied before BCG vaccination, as well as after 2 months and 1 y. In the primary-vaccinated as well as the revaccinated group the LT response increased after 2 months and remained significantly higher than baseline values after 1 y. In both groups the interferon-gamma (IFN-gamma) levels increased significantly after 2 months and the increase was maintained after 1 y. LT increased more in the revaccinated group than in the primary-vaccinated group, while the increase in IFN-gamma response did not differ between the 2 groups. Both primary vaccination and revaccination of TST negative young adults caused a significant increase in the T-helper 1-type immune response, suggesting a protective effect against TB. The present in vitro results thus support the policy in several low-endemic countries of primary vaccination as well as revaccination of young adults at risk of TB exposure.
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| 48. |
- Flodin, Mats, et al.
(författare)
-
Evaluation of Dade Behring N Latex Cystatin C reagent on Abbott ci8200
- 2006
-
Ingår i: Upsala Journal of Medical Sciences. - 0300-9734 .- 2000-1967. ; 111:2, s. 209-213
-
Tidskriftsartikel (refereegranskat)abstract
- Plasma cystatin C has been shown in several studies to be superior to plasma creatinine for estimation of glomerular filtration rate (GFR). Reporting cystatin C results in mL/min using conversion formulas for transforming cystatin C expressed as mg/L to GFR expressed as mL/min has greatly facilitated the clinical use of the marker. At our hospital we have an increasing demand for cystatin C and at present we perform over 1,400 cystatin C analyses a month. The test is available at all hours. This in combination with the volume emphasises the need to have the assay close to the routine chemistry instrument to reduce handling time per test and time to report test results. We have thus evaluated the Dade Behring N Latex Cystatin C assay (Dade Behring, Deerfield, IL, USA) on Architect 68200 (Abbott Laboratories, Abbott Park, IL, USA). The nephelometric method on the ProSpec (Dade Behring) and the turbidimetric method on Architect 68200 showed very good agreement (y = 1.0072x + 0.0042; R-2 = 0.987). Accordingly, running the cystatin C analyses on a chemistry instrument (Architect 68200) would be proper to increase the availability of the analysis and reduce turnaround times.
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| 49. |
- Flodin, Mats, et al.
(författare)
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Variations in assay protocol for the Dako cystatin C method may change patient results by 50% without changing the results for controls
- 2006
-
Ingår i: Clinical Chemistry and Laboratory Medicine. - 1434-6621 .- 1437-4331. ; 44:12, s. 1481-1485
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Cystatin C is increasingly used as a glomerular filtration marker, but so far only a few companies produce most of the cystatin C reagents suited for turbidimetry or nephelometry use in clinical laboratories. Methods: We studied different protocols for measuring cystatin C on an Architect ci8200 system using cystatin C reagents from Dako (Glostrup, Denmark). The results were compared with those obtained with Dade Behring reagents (Deerfield, IL, USA) on a BN ProSpec system. Results: Differences in assay protocol on the same instrument with the Dako reagent yielded an up to 50% difference in glomerular filtration rate calculated from the cystatin C results when analyzing patient samples, but had no effect on the results for controls. There were also significant differences regarding linearity and kinetics between samples and controls/calibrators. Conclusions: The results indicate different reactivity of the Dako antibodies against calibrators and controls in comparison with patient samples, highlighting the importance of using controls and calibrators that do not differ from patient samples.
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| 50. |
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