| 1. |
- Dwibedi, Chinmay Kumar, et al.
(författare)
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Biological amplification of low frequency mutations unravels laboratory culture history of the bio-threat agent Francisella tularensis
- 2020
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Ingår i: Forensic Science International. - : Elsevier. - 1872-4973 .- 1878-0326. ; 45
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Tidskriftsartikel (refereegranskat)abstract
- Challenges of investigating a suspected bio attack include establishing if microorganisms have been cultured to produce attack material and to identify their source. Addressing both issues, we have investigated genetic variations that emerge during laboratory culturing of the bacterial pathogen Francisella tularensis. Key aims were to identify genetic variations that are characteristic of laboratory culturing and explore the possibility of using biological amplification to identify genetic variation present at exceedingly low frequencies in a source sample. We used parallel serial passage experiments and high-throughput sequencing of F. tularensis to explore the genetic variation. We found that during early laboratory culture passages of F. tularensis, gene duplications emerged in the pathogen genome followed by single-nucleotide polymorphisms in genes for bacterial capsule synthesis. Based on a biological enrichment scheme and the use of high-throughput sequencing, we identified genetic variation that likely pre-existed in a source sample. The results support that capsule synthesis gene mutations are common during laboratory culture, and that a biological amplification strategy is useful for linking a F. tularensis sample to a specific laboratory variant among many highly similar variants.
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| 2. |
- Pettersson, Karl, 1983-, et al.
(författare)
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Simulation Aided Design and Testing of Hydromechanical Transmissions
- 2014
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Ingår i: The 9th JFPS International Symposium on Fluid Power, Matsue, 2014. - 4931070108
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Konferensbidrag (refereegranskat)abstract
- This paper demonstrates the use of high-speed simulation in transmission conceptual design and presents a transmission test bed for hardware-in-the-loop simulations of hydromechanical transmission concepts. Complex transmissions, such as multiple-mode hydromechanical transmissions and hydraulic hybrid transmissions, present new difficulties and costs in the development process. There is today a greater demand for more efficient product development and more work has shifted towards simulation. The Hopsan simulation package allows robust, high-speed simulations suitable for both offline and hardware-in-the-loop simulation. New simulation models for hydromechanical transmissions are developed and used to simulate a known two-mode transmission concept. The same concept is also tested in hardware-in-the-loop simulations in the proposed transmission test bed. Results show good agreement with the hardware tests and highlight the proficiency of the simulation tools.
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| 3. |
- Szczerba, Krzysztof, 1985, et al.
(författare)
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Impact of Forward Error Correction on Energy Consumption of VCSEL-based Transmitters
- 2015
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Ingår i: 41st European Conference on Optical Communication, ECOC 2015, Valencia, Spain, 27 September - 1 October 2015. - 9788460817413 ; 2015-November
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Konferensbidrag (refereegranskat)abstract
- We present an analysis of the effect of FEC on energy consumption of VCSEL based transmitters for short-range optical links. We show that a low-complexity FEC can reduce the overall energy consumption of the transmitter.
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| 4. |
- Ahlinder, Jon, et al.
(författare)
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Increased knowledge of Francisella genus diversity highlights the benefits of optimised DNA-based assays
- 2012
-
Ingår i: BMC Microbiology. - : Springer Science and Business Media LLC. - 1471-2180. ; 12, s. 220-
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Tidskriftsartikel (refereegranskat)abstract
- Background: Recent advances in sequencing technologies offer promising tools for generating large numbers of genomes, larger typing databases and improved mapping of environmental bacterial diversity. However, DNA-based methods for the detection of Francisella were developed with limited knowledge about genetic diversity. This, together with the high sequence identity between several Francisella species, means there is a high risk of false identification and detection of the highly virulent pathogen Francisella tularensis. Moreover, phylogenetic reconstructions using single or limited numbers of marker sequences often result in incorrect tree topologies and inferred evolutionary distances. The recent growth in publicly accessible whole-genome sequences now allows evaluation of published genetic markers to determine optimal combinations of markers that minimise both time and laboratory costs. Results: In the present study, we evaluated 38 previously published DNA markers and the corresponding PCR primers against 42 genomes representing the currently known diversity of the genus Francisella. The results highlight that PCR assays for Francisella tularensis are often complicated by low specificity, resulting in a high probability of false positives. A method to select a set of one to seven markers for obtaining optimal phylogenetic resolution or diagnostic accuracy is presented. Conclusions: Current multiple-locus sequence-typing systems and detection assays of Francisella, could be improved by redesigning some of the primers and reselecting typing markers. The use of only a few optimally selected sequence-typing markers allows construction of phylogenetic topologies with almost the same accuracy as topologies based on whole-genome sequences.
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| 5. |
- Aiempanakit, Montri, et al.
(författare)
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Effect of peak power in reactive high power impulse magnetron sputtering of titanium dioxide
- 2011
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Ingår i: Surface & Coatings Technology. - : Elsevier BV. - 0257-8972 .- 1879-3347. ; 205:20, s. 4828-4831
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Tidskriftsartikel (refereegranskat)abstract
- The effect of peak power in a high power impulse magnetron sputtering (HiPIMS) reactive deposition of TiO(2) films has been studied with respect to the deposition rate and coating properties. With increasing peak power not only the ionization of the sputtered material increases but also their energy. In order to correlate the variation in the ion energy distributions with the film properties, the phase composition, density and optical properties of the films grown with different HiPIMS-parameters have been investigated and compared to a film grown using direct current magnetron sputtering (DCMS). All experiments were performed for constant average power and pulse on time (100W and 35 mu s, respectively), different peak powers were achieved by varying the frequency of pulsing. Ion energy distributions for Ti and O and its dependence on the process conditions have been studied. It was found that films with the highest density and highest refractive index were grown under moderate HiPIMS conditions (moderate peak powers) resulting in only a small loss in mass-deposition rate compared to DCMS. It was further found that TiO2 films with anatase and rutile phases can be grown at room temperature without substrate heating and without post-deposition annealing.
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| 6. |
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| 7. |
- Aiempanakit, Montri, et al.
(författare)
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Hysteresis and process stability in reactive high power impulse magnetron sputtering of metal oxides
- 2011
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Ingår i: Thin Solid Films. - : Elsevier BV. - 0040-6090 .- 1879-2731. ; 519:22, s. 7779-7784
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Tidskriftsartikel (refereegranskat)abstract
- In the further development of reactive sputter deposition, strategies which allow for stabilization of the transition zone between the metallic and compound modes, elimination of the process hysteresis, and increase of the deposition rate, are of particular interest. In this study, the hysteresis behavior and the characteristics of the transition zone during reactive high power impulse magnetron sputtering (HiPIMS) of Al and Ce targets in an Ar-O(2) atmosphere as a function of the pulsing frequency and the pumping speed are investigated. Comparison with reactive direct current magnetron sputtering (DCMS) reveals that HiPIMS allows for elimination/suppression of the hysteresis and a smoother transition from the metallic to the compound sputtering mode. For the experimental conditions employed in the present study, optimum behavior with respect to the hysteresis width is obtained at frequency values between 2 and 4 kHz, while HiPIMS processes with values below or above this range resemble the DCMS behavior. Al-O films are deposited using both HiPIMS and DCMS. Analysis of the film properties shows that elimination/suppression of the hysteresis in HiPIMS facilitates the growth of stoichiometric and transparent Al(2)O(3) at relatively high deposition rates over a wider range of experimental conditions as compared to DCMS.
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| 8. |
- Aijaz, Asim, et al.
(författare)
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Dual-magnetron open field sputtering system for sideways deposition of thin films
- 2010
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Ingår i: SURFACE and COATINGS TECHNOLOGY. - : Elsevier BV. - 0257-8972. ; 204:14, s. 2165-2169
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Tidskriftsartikel (refereegranskat)abstract
- A dual-magnetron system for deposition inside tubular substrates has been developed. The two magnetrons are facing each other and have opposing magnetic fields forcing electrons and thereby also ionized material to be transported radially towards the substrate. The depositions were made employing direct current magnetron sputtering (DCMS) and high power impulse magnetron sputtering (HiPIMS). To optimize the deposition rate, the system was characterized at different separation distances between the magnetrons under the same sputtering conditions. The deposition rate is found to increase with increasing separation distance independent of discharge technique. The emission spectrum from the HiPIMS plasma shows a highly ionized fraction of the sputtered material. The electron densities of the order of 10(16) m(-3) and 10(18) m(-3) have been determined in the DCMS and the HiPIMS plasma discharges respectively. The results demonstrate a successful implementation of the concept of sideways deposition of thin films providing a solution for coating complex shaped surfaces.
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| 9. |
- Andersson, Petter, et al.
(författare)
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A case study of how knowledge based engineering tools support experience re-use
- 2011
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Ingår i: Research into Design. - Bangalore, India : Research Publishing Services. - 9789810877217
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Konferensbidrag (refereegranskat)abstract
- A manufacturing company’s unique intellectual capital is to a large extent built on experience from its own product development and manufacturing processes. Thus, methods and tools to utilize and benefit from this experience in an efficient way have an impact on a company’s ability to stay competitive and advance on the global market. Knowledge Based Engineering (KBE) is an engineering methodology to capture engineering knowledge systematically into the design system. Hence, KBE tools are considered to support experience re-use and improve engineering activities. This paper presents the results from a study where the objective was to investigate the support for experience re-use in KBE applications in an aerospace company. A proposed framework is presented to analyze the capturing and use of experience in a company’s processes identifying gaps and propose improvements. The study revealed weaknesses in the process steps for experience feedback which can be used to improve KBE applications further.
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| 10. |
- Andersson, Petter, et al.
(författare)
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Project: Robust Machining
- 2010
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Annan publikation (populärvet., debatt m.m.)abstract
- New generations of environmentally friendly and safe vehicles require manufacturing of light weight materials with higher strength and, as a consequence, tougher machining conditions and increased machining robustness. There is a lack of practical and reliable methods and tools to meet these challenges in the automotive industry. The very complex system of machine tool, fixture, cutting tools and the machined part is almost impossible to model without complementary measurements of the real system at the factory floor in full production. Using Virtual Engineering together with studies of machining processes the project team will assess these challenges.
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| 11. |
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| 12. |
- Asp, Petter, et al.
(författare)
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25 kap. Om böter m.m.
- 2018
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Ingår i: Brottsbalken. - Stockholm : Karnov Group. - 9789176106686 ; , s. 1211-1231
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 13. |
- Asp, Petter, et al.
(författare)
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25 kap. Om böter m.m.
- 2018
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Ingår i: Brottsbalken. - Stockholm : Karnov Group. - 9789176106686 ; , s. 1211-1231
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 14. |
- Asp, Petter, et al.
(författare)
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26 kap. Om fängelse
- 2018
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Ingår i: Brottsbalken. - Stockholm : Karnov Group. - 9789176106686 ; , s. 1233-1276
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 15. |
- Aurell, Emelie, et al.
(författare)
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Mikroplaster : Redovisning av regeringsuppdrag om källor till mikroplaster och förslag på åtgärder för minskade utsläpp i Sverige
- 2017
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- I augusti 2015 fick Naturvårdsverket i uppdrag från regeringen att identifiera viktigare källor i Sverige till utsläpp av mikroplaster till havet och verka för att reducera utsläppen från dessa källor. I den här rapporten redovisar Naturvårdsverket uppdraget. Vi presenterar resultaten från den första, övergripande kartläggningen av källor till och spridning av mikroplaster i Sverige, en bedömning av vilka av de kartlagda källorna som primärt bör åtgärdas samt vilka steg som behöver tas för att förebygga utsläpp och minska spridning av mikroplaster till hav, sjöar och vattendrag från dessa källor.Förekomsten av mikroplast i den marina miljön har uppmärksammats allt mer under senare år, inte minst på global nivå. Mikroplast är ett samlingsnamn för små, små plastfragment (1 nm till 5 mm). De mikroplaster som hittats i världshaven, men även i sötvattensystem, har olika ursprung. Mikroplast kan bildas oavsiktligt när plastföremål slits och plastpartiklar frigörs, eller när vi inte återanvänder, återvinner eller slänger plastmaterial på rätt sätt utan plasten blir skräp som succesivt bryts ned till mindre och mindre bitar i naturen. Det finns också plast som från början tillverkas som små pellets eller korn.Utgångspunkten för arbetet har varit miljökvalitetsmålen Hav i balans samt levande kust och Levande sjöar och vattendrag samt målet om Giftfri miljö. Reduceradeutsläpp av mikroplaster till hav, sjöar och vattendrag bidrar till att nå dessa mål.Uppdraget har genomförts av Naturvårdsverket i samarbete med Havs- och vattenmyndigheten, andra berörda myndigheter, samt med deltagande av berörda organisationer och andra intressenter mellan augusti 2015 och maj 2017. Slutsatserna är Naturvårdsverkets egna.
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| 16. |
- Bains, Sandra, et al.
(författare)
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Plastid Molecular Pharming II. Production of Biopharmaceuticals by Plastid Transformation
- 2017
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Ingår i: Mini-Reviews in Medicinal Chemistry. - : Bentham Science Publishers Ltd.. - 1389-5575. ; 17:13, s. 1316-1330
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Tidskriftsartikel (refereegranskat)abstract
- Background: Higher plants have been used in medicine throughout human history. Method: While traditional medicinal uses relied on compounds produced naturally by plants, recent advances have enabled the use of plant-based factories to produce diverse agents including pharmaceuticals, antibiotics, and vaccines. The genes responsible for the production of these substances can be either transiently expressed in plants or integrated into their nuclear genome or plastid genome (plastome) by genetic transformation. This review focuses on the application of plastid transformation of higher plants to produce biopharmaceuticals for human applications that are neither antibiotics nor vaccines. Plastid transformation has several advantages over nuclear transformation and represents a minimal risk of transgene contamination to the environment via pollen grains because plastid genes are in most species normally maternally inherited and thus absent from pollen. Other advantages of sitedirected plastid insertion via homologous recombination include strong gene expression due to the plastid genome's high copy number and resistance to silencing, and the ability to achieve multi-gene expression with a single insertion step. Results: Compared to bacterial systems, plant-based bioreactors offer lower production costs, lower risks of human pathogen contamination, and the possibility of exploiting post-translational modification. Conclusion: Consequently, sustainable plant systems based on different species, plastids, and tissues could become an important source of added value in pharmaceutical production.
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| 17. |
- Baken, S., et al.
(författare)
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Ageing of vanadium in soils and consequences for bioavailability
- 2012
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Ingår i: European Journal of Soil Science. - : Elsevier. - 1351-0754 .- 1365-2389. ; 63:6, s. 839-847
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Tidskriftsartikel (refereegranskat)abstract
- Total vanadium (V) concentrations in soils commonly range from 20 to 120 mg kg-1. Vanadium added directly to soils is more soluble than geogenic V and can be phytotoxic at doses within this range of background concentrations. However, it is unknown how slow sorption reactions change the fate and effect of added V in soils. This study addresses the changes in V solubility, toxicity and bioavailability in soils over time. Four soils were amended with pentavalent V in the form of a soluble vanadate salt, and extractable V concentrations were monitored over 100 days. The toxicity to barley and tomato plants was evaluated in freshly spiked soils and in the corresponding aged soils that were equilibrated for up to 330 days after spiking. The V concentrations in 0.01 m CaCl2 soil extracts decreased approximately two-fold between 14 and 100 days after soil spiking, and the reaction kinetics were similar for all soils. The phytotoxicity of added V decreased on average two-fold between freshly spiked and aged soils. The reduced toxicity was associated with a corresponding decrease in V concentrations in the isolated soil solutions and in the shoots. The V speciation in the soil solution of the aged soils was dominated by V(V); less than 8% was present as V(IV). Oxalate extractions suggest that the V(V) added to soils is predominantly sorbed onto poorly crystalline oxyhydroxides. It is concluded that the toxicity of V measured in freshly spiked soils may not be representative of soils subject to a long-term V contamination in the field.
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| 18. |
- Bamiedakis, Nikos, et al.
(författare)
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25 Gb/s data transmission over a 1.4 m long multimode polymer spiral waveguide
- 2014
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Ingår i: Conference on Lasers and Electro-Optics Europe - Technical Digest. - Washington, D.C. : OSA. - 9781557529992
-
Konferensbidrag (refereegranskat)abstract
- Data transmission studies of a 1.4m long multimode polymer spiral waveguide using an 850nm VCSEL are presented. Error-free 25 Gb/s data transmission is demonstrated over that waveguide length, achieving a record bandwidth-length product of 21GHz×m.
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| 19. |
- Bamiedakis, N., et al.
(författare)
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40 Gb/s data transmission over a 1 m long multimode polymer spiral waveguide
- 2014
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Ingår i: European Conference on Optical Communication, ECOC 2014; Cannes; France; 21 September 2014 through 25 September 2014. - 9782954944401 ; , s. Paper P.4.7-
-
Konferensbidrag (refereegranskat)abstract
- We report record error-free data transmission of 40Gb/s over a 1m-long multimode polymer spiral waveguide. The waveguide imposes no significant transmission impairments in the link despite its highly-multimoded nature and long length, demonstrating its potential in high-speed board-level optical interconnections.
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| 20. |
- Bamiedakis, N., et al.
(författare)
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40 Gb/s Data Transmission Over a 1-m-Long Multimode Polymer Spiral Waveguide for Board-Level Optical Interconnects
- 2015
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Ingår i: Journal of Lightwave Technology. - 0733-8724 .- 1558-2213. ; 33:4, s. 882-888
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Tidskriftsartikel (refereegranskat)abstract
- Optical interconnects have attracted considerable attention for use in short-reach communication links within high-performance electronic systems, such as data centers, supercomputers, and data storage systems. Multimode polymer waveguides, in particular, constitute an attractive technology for use in board-level interconnects as they can be cost-effectively integrated onto standard PCBs and allow system assembly with relaxed alignment tolerances. However, their highly multimoded nature raises important concerns about their bandwidth limitations and their potential to support very high on-board data rates. In this paper, we report record error-free (BER
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| 21. |
- Bamiedakis, N., et al.
(författare)
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56 Gb/s PAM-4 data transmission over a 1 m long multimode polymer interconnect
- 2015
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Ingår i: CLEO: Science and Innovations, CLEO-SI 2015, San Jose; United States; 10-15 May 2015. - 9781557529688 ; , s. 2267-
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Konferensbidrag (refereegranskat)abstract
- Advanced modulation formats can enable >40 Gb/s data rates in waveguide-based optical interconnects without the need for high-specification optoelectronic components. Record 56Gb/s PAM-4 data transmission is demonstrated over a 1 m-long multimode polymer waveguide.
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| 22. |
- Bandaru, Manoj Kumar, et al.
(författare)
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Zebrafish larvae as a model system for systematic characterization of drugs and genes in dyslipidemia and atherosclerosis
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Tidskriftsartikel (refereegranskat)abstract
- Background: Hundreds of loci have been robustly associated with circulating lipids, atherosclerosis and coronary artery disease; but for most loci the causal genes and mechanisms remain uncharacterized.Methods: We developed a semi-automated experimental pipeline for systematic, quantitative, large-scale characterization of mechanisms, drugs and genes associated with dyslipidemia and atherosclerosis in a zebrafish model system. We validated our pipeline using a dietary (n>2000), drug treatment (n>1000), and genetic intervention (n=384).Results: Our results show that five days of overfeeding and cholesterol supplementation had independent pro-atherogenic effects, which could be diminished by concomitant treatment with atorvastatin and ezetimibe. CRISPR-Cas9-induced mutations in orthologues of proof-of-concept genes resulted in higher LDL cholesterol levels (apoea), and more early stage atherosclerosis (apobb.1).Conclusions: In summary, our pipeline facilitates systematic, in vivo characterization of drugs and candidate genes to increase our understanding of disease etiology, and can likely help identify novel targets for therapeutic intervention.
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| 23. |
- Baveja, Prashant, 1985, et al.
(författare)
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Impact of device parameters on thermal performance of high speed oxide confined 850 nm VCSELs
- 2012
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Ingår i: IEEE Journal of Quantum Electronics. - 0018-9197 .- 1558-1713. ; 48:1, s. 17-26
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Tidskriftsartikel (refereegranskat)abstract
- We study the impact of device parameters, such asinner-aperture diameter and cavity photon lifetime, on thermal rollover mechanisms in 850-nm, oxide-confined, vertical-cavity surface-emitting lasers (VCSELs) designed for high-speed operation. We perform measurements on four different VCSELs of different designs and use our empirical thermal model for calculating the power dissipated with increasing bias currents through various physical processes such as absorption within the cavity, carrier thermalization, carrier leakage, spontaneous carrier recombination, and Joule heating. When reducing the topmirror reflectivity to reduce internal optical absorption loss we find an increase of power dissipation due to carrier leakage. There is therefore a trade-ff between the powers dissipated owing to optical absorption and carrier leakage in the sense that overcompensating for optical absorption enhances carrier leakage (and vice versa). We further find that carrier leakage places the ultimate limit on the thermal performance for this entire class ofdevices. Our analysis yields useful design optimization strategies for mitigating the impact of carrier leakage and should thereby prove useful for the performance enhancement of 850-nm, highspeed, oxide-confined VCSELs.
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| 24. |
- Baveja, P. P., et al.
(författare)
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Assessment of VCSEL thermal rollover mechanisms from measurements and empirical modeling
- 2011
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Ingår i: Optics Express. - 1094-4087 .- 1094-4087. ; 19:16, s. 15490-15505
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Tidskriftsartikel (refereegranskat)abstract
- We use an empirical model together with experimental measurements for studying mechanisms contributing to thermal rollover in vertical-cavity surface-emitting lasers (VCSELs). The model is based on extraction of the temperature dependence of threshold current, internal quantum efficiency, internal optical loss, series resistance and thermal impedance from measurements of output power, voltage and lasing wavelength as a function of bias current over an ambient temperature range of 15-100 degrees C. We apply the model to an oxide-confined, 850-nm VCSEL, fabricated with a 9-mu m inner-aperture diameter and optimized for highspeed operation, and show for this specific device that power dissipation due to linear power dissipation (sum total of optical absorption, carrier thermalization, carrier leakage and spontaneous carrier recombination) exceeds power dissipation across the series resistance (quadratic power dissipation) at any ambient temperature and bias current. We further show that the dominant contributors to self-heating for this particular VCSEL are quadratic power dissipation, internal optical loss, and carrier leakage. A rapid reduction of the internal quantum efficiency at high bias currents (resulting in high temperatures) is identified as being the major cause of thermal rollover. Our method is applicable to any VCSEL and is useful for identifying the mechanisms limiting the thermal performance of the device and to formulate design strategies to ameliorate them.
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| 25. |
- Baveja, P. P., et al.
(författare)
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Impact of photon lifetime on thermal rollover in 850-nm high-speed VCSELs
- 2012
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Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 0277-786X .- 1996-756X. - 9780819489197 ; 8276
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Konferensbidrag (refereegranskat)abstract
- We present an empirical thermal model for VCSELs based on extraction of temperature dependence of macroscopic VCSEL parameters from CW measurements. We apply our model to two, oxide-confined, 850-nm VCSELs, fabricated with a 9-mu m inner-aperture diameter and optimized for high-speed operation. We demonstrate that for both these devices, the power dissipation due to linear heat sources dominates the total self-heating. We further show that reducing photon lifetime down to 2 ps drastically reduces absorption heating and improves device static performance by delaying the onset of thermal rollover. The new thermal model can identify the mechanisms limiting the thermal performance and help in formulating the design strategies to ameliorate them.
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| 26. |
- Bengtsson, Petter, 1976-
(författare)
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Buyers and Sellers on the Stockholm Housing Market
- 2023
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Buying and selling housing is for most a very important event that can strongly affect their financial situation. At the same time homes are recognized as places with great importance above and beyond financial matters and in everyday talk and in the previous literature homes are described as safe-havens, status symbols, influencing networks of friends and acquaintances, canvasses for projecting identity, tied to gentrification, segregation and much more. Previous literature tends to separate and focus on either economic/financial aspects or more symbolic values in housing, and/or separate its analysis to either owners of homes or buyers of homes. This study bridges the restricted focus in the previous literature by analyzing both buying and selling housing from an economical sociological viewpoint. The market and case is buying and selling housing in the County of Stockholm and since often the same household appear on both sides of the market close in time, a switch-role activity, the notion of switching and how the two roles relate to one and the same actor is central to explain. The explanation provided is further warranted in that this type of market, understudied in all types of previous literature, is a type of market which, not at least via internet platforms, is growing in volume. By ethnographic work, interviews and observations, buyers and sellers of housing are studied and analyzed. The theoretical concept of modes is used to create an understanding and explanation of housing buyers’ and sellers’ actions. The three research questions; what buyers and sellers do, how they do it, and why they do it are tied to the mode apparatus. Buyers are found to be disperse as their mode displays are plenty and varied. Some buyers are committed and certain of what they want in the future home and what the right price is, while some are eager to learn and find out what a good housing deal is. Others display modes of dreaming of future homes, play shop or try to learn what the proper way to buy housing really is or should be. Sellers are found to be much more coherent as group and as one mode display. The relatively set way in which sellers are provided a script on what to do and how to do it make them remarkably conformist. Sellers are found to show great trust in the institutional practice of the housing market. The lack of personal and subjective displays of identity and taste stand out. Sellers are found to display a general mode of involution, where culture and style tends to be ever watered down. Comparing the two sides of the market, buying and selling housing are therefore understood as two very different activities housed under one roof. Many actors appear on both sides of the market, as both buyer and seller close in time, but what the actor subjectively value as buyer and what influence their mode display, is not relevant when switching to selling. What is won for the buyer in the sense of having a distinct subjective mode is lost in uncertainty about what is the best deal on a unit of housing. Sellers on the other hand have little ability to display any real image and identity in their home for sale. However, what is lost in not having a distinct subjective mode display is then won in the certainty that following the script will secure the best possible deal. The study’s results point towards the value of further empirical work on switch-role markets to provide an extended knowledge of what is found here.
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| 27. |
- Berggren, Hanna, et al.
(författare)
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Effects of environmental translocation and host characteristics on skin microbiomes of sun-basking fish
- 2023
-
Ingår i: Proceedings of the Royal Society of London. Biological Sciences. - : Royal Society. - 0962-8452 .- 1471-2954. ; 290:2013
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Tidskriftsartikel (refereegranskat)abstract
- Variation in the composition of skin-associated microbiomes has been attributed to host species, geographical location and habitat, but the role of intraspecific phenotypic variation among host individuals remains elusive. We explored if and how host environment and different phenotypic traits were associated with microbiome composition. We conducted repeated sampling of dorsal and ventral skin microbiomes of carp individuals (Cyprinus carpio) before and after translocation from laboratory conditions to a semi-natural environment. Both alpha and beta diversity of skin-associated microbiomes increased substantially within and among individuals following translocation, particularly on dorsal body sites. The variation in microbiome composition among hosts was significantly associated with body site, sun-basking, habitat switch and growth, but not temperature gain while basking, sex, personality nor colour morph. We suggest that the overall increase in the alpha and beta diversity estimates among hosts were induced by individuals expressing greater variation in behaviours and thus exposure to potential colonizers in the pond environment compared with the laboratory. Our results exemplify how biological diversity at one level of organization (phenotypic variation among and within fish host individuals) together with the external environment impacts biological diversity at a higher hierarchical level of organization (richness and composition of fish-associated microbial communities).
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| 28. |
- Berggren, Hanna, et al.
(författare)
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Fish skin microbiomes are highly variable among individuals and populations but not within individuals
- 2022
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Ingår i: Frontiers in Microbiology. - : Frontiers Media S.A.. - 1664-302X. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- Fish skin-associated microbial communities are highly variable among populations and species and can impact host fitness. Still, the sources of variation in microbiome composition, and particularly how they vary among and within host individuals, have rarely been investigated. To tackle this issue, we explored patterns of variation in fish skin microbiomes across different spatial scales. We conducted replicate sampling of dorsal and ventral body sites of perch (Perca fluviatilis) from two populations and characterized the variation of fish skin-associated microbial communities with 16S rRNA gene metabarcoding. Results showed a high similarity of microbiome samples taken from the left and right side of the same fish individuals, suggesting that fish skin microbiomes can be reliably assessed and characterized even using a single sample from a specific body site. The microbiome composition of fish skin differed markedly from the bacterioplankton communities in the surrounding water and was highly variable among individuals. No ASV was present in all samples, and the most prevalent phyla, Actinobacteria, Bacteroidetes, and Proteobacteria, varied in relative abundance among fish hosts. Microbiome composition was both individual- and population specific, with most of the variation explained by individual host. At the individual level, we found no diversification in microbiome composition between dorsal and ventral body sites, but the degree of intra-individual heterogeneity varied among individuals. To identify how genetic and phenotypic characteristics of fish hosts impact the rate and nature of intra-individual temporal dynamics of the skin microbiome, and thereby contribute to the host-specific patterns documented here, remains an important task for future research.
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| 29. |
- Berggren, Hanna, et al.
(författare)
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Testing for local adaptation to spawning habitat in sympatric subpopulations of northern pike by reciprocal translocation of embryos
- 2016
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 11:5
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Tidskriftsartikel (refereegranskat)abstract
- We tested for local adaption in early life-history traits by performing a reciprocal translocation experiment with approximately 2500 embryos of pike (Esox lucius) divided in paired split-family batches. The experiment indicated local adaptation in one of the two subpopulations manifested as enhanced hatching success of eggs in the native habitat, both when compared to siblings transferred to a non-native habitat, and when compared to immigrant genotypes from the other subpopulation. Gene-by-environment effects on viability of eggs and larvae were evident in both subpopulations, showing that there existed genetic variation allowing for evolutionary responses to divergent selection, and indicating a capacity for plastic responses to environmental change. Next, we tested for differences in female life-history traits. Results uncovered that females from one population invested more resources into reproduction and also produced more (but smaller) eggs in relation to their body size compared to females from the other population. We suggest that these females have adjusted their reproductive strategies as a counter-adaptation because a high amount of sedimentation on the eggs in that subpopulations spawning habitat might benefit smaller eggs. Collectively, our findings point to adaptive divergence among sympatric subpopulations that are physically separated only for a short period during reproduction and early development – which is rare. These results illustrate how combinations of translocation experiments and field studies of life-history traits might infer about local adaptation and evolutionary divergence among populations. Local adaptations in subdivided populations are important to consider in management and conservation of biodiversity, because they may otherwise be negatively affected by harvesting, supplementation, and reintroduction efforts targeted at endangered populations.
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| 30. |
- Berggren, Lars, et al.
(författare)
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Bomben som skakade Sverige
- 2008
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Ingår i: Ekot från Amalthea. - 9789163332517 ; , s. 19-64
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 31. |
- Bergqvist, Filip, et al.
(författare)
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Inhibition of mPGES-1 or COX-2 Results in Different Proteomic and Lipidomic Profiles in A549 Lung Cancer Cells
- 2019
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Ingår i: Frontiers in Pharmacology. - : Frontiers Media SA. - 1663-9812. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- Pharmacological inhibition of microsomal prostaglandin E synthase (mPGES)-1 for selective reduction in prostaglandin E-2 (PGE(2)) biosynthesis is protective in experimental models of cancer and inflammation. Targeting mPGES-1 is envisioned as a safer alternative to traditional non-steroidal anti-inflammatory drugs (NSAIDs). Herein, we compared the effects of mPGES-1 inhibitor Compound III (CIII) with the cyclooxygenase (COX)-2 inhibitor NS-398 on protein and lipid profiles in interleukin (IL)-1 beta-induced A549 lung cancer cells using mass spectrometry. Inhibition of mPGES-1 decreased PGE(2) production and increased PGF(2 alpha) and thromboxane B-2 (TXB2) formation, while inhibition of COX-2 decreased the production of all three prostanoids. Our proteomics results revealed that CIII downregulated multiple canonical pathways including eIF2, eIF4/P70S6K, and mTOR signaling, compared to NS-398 that activated these pathways. Moreover, pathway analysis predicted that CIII increased cell death of cancer cells (Z = 3.8, p = 5.1E-41) while NS-398 decreased the same function (Z = -5.0, p = 6.5E-35). In our lipidomics analyses, we found alterations in nine phospholipids between the two inhibitors, with a stronger alteration in the lysophospholipid (LPC) profile with NS-398 compared to CIII. Inhibition of mPGES-1 increased the concentration of sphinganine and dihydroceramide (C16:0D hCer), while inhibition of COX-2 caused a general decrease in most ceramides, again suggesting different effects on cell death between the two inhibitors. We showed that CIII decreased proliferation and potentiated the cytotoxic effect of the cytostatic drugs cisplatin, etoposide, and vincristine when investigated in a live cell imaging system. Our results demonstrate differences in protein and lipid profiles after inhibition of mPGES-1 or COX-2 with important implications on the therapeutic potential of mPGES-1 inhibitors as adjuvant treatment in cancer. We encourage further investigations to illuminate the clinical benefit of mPGES-1 inhibitors in cancer.
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| 32. |
- Bergström, Kristofer, et al.
(författare)
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Exceptional longevity in northern peripheral populations of Wels catfish (Siluris glanis)
- 2022
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Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 12:1
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Tidskriftsartikel (refereegranskat)abstract
- Studies of life-history variation across a species range are crucial for ecological understanding and successful conservation. Here, we examined the growth and age of Wels catfish (Silurus glanis) in Sweden, which represent the northernmost populations in Europe. A total of 1183 individuals were captured, marked and released between 2006 and 2020. Mark-recapture data from 162 individuals (size range: 13-195 cm) were used to estimate von Bertalanffy growth curve parameters which revealed very slow growth rates compared to catfish within the core distribution area (central Europe). The fitted von Bertalanffy growth curve predicted a 150 cm catfish to be around 40 years old, while the largest recaptured individual (length 195 cm) was estimated to be 70 (95% CI 50-112) years old. This was substantially older than the previously documented maximum age of a catfish. The weight at length relationships in these northern peripheral populations were similar to those documented for catfish in central Europe indicating that resources did not constrain growth. This indicates that the slow growth and exceptional high age in the northern catfish populations are the result of lower temperatures and/or local adaptations.
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| 33. |
- Berndtsson, Ronny, et al.
(författare)
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Drivers of changing urban flood risk : A framework for action
- 2019
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Ingår i: Journal of Environmental Management. - : Elsevier. - 0301-4797 .- 1095-8630. ; 240, s. 47-56
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Tidskriftsartikel (refereegranskat)abstract
- This study focuses on drivers for changing urban flood risk. We suggest a framework for guiding climate change adaptation action concerning flood risk and manageability in cities. The identified key drivers of changing flood hazard and vulnerability are used to provide an overview of each driver's impact on flood risk and manageability at the city level. We find that identified drivers for urban flood risk can be grouped in three different priority areas with different time horizon. The first group has high impact but is manageable at city level. Typical drivers in this group are related to the physical environment such as decreasing permeability and unresponsive engineering. The second group of drivers is represented by public awareness and individual willingness to participate and urbanization and urban sprawl. These drivers may be important and are manageable for the cities and they involve both short-term and long-term measures. The third group of drivers is related to policy and long-term changes. This group is represented by economic growth and increasing values at risk, climate change, and increasing complexity of society. They have all high impact but low manageability. Managing these drivers needs to be done in a longer time perspective, e.g., by developing long-term policies and exchange of ideas.
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| 34. |
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| 35. |
- Braun, Robert, 1984-, et al.
(författare)
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Hopsan: An Open-Source Tool for Rapid Modelling and Simulation of Fluid and Mechatronic Systems
- 2020
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Ingår i: <em>Proceedings of the BATH/ASME 2020 Symposium on Fluid Power and Motion Control</em>. - 9780791883754
-
Konferensbidrag (refereegranskat)abstract
- Hopsan is an open-source simulation package developed as a collaboration project between industry and academia. The simulation methodology is based on transmission line modelling, which provides several benefits such as linear model scalability, numerical robustness and parallel simulation. All sub-models are pre-compiled, so that no compilation is required prior to starting a simulation. Default component libraries are available for hydraulic, mechanic, pneumatic, electric and signal domains. Custom components can be written in C++ or generated from Modelica and Mathematica. Support for simulation-based optimization is provided using population-based, evolutionary or direct-search algorithms. Recent research has largely focused on co-simulation with other simulation tools. This is achieved either by using the Functional Mock-up Interface standard, or by tool-to-tool communications. This paper provides a description of the program and its features, the current status of the project, and an overview of recent and ongoing use cases from industry and academia.
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| 36. |
- Castro, Jose Manuel, et al.
(författare)
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48.7 Gb/s 4-PAM Transmission over 200 m of High Bandwidth MMF using an 850 nm VCSEL
- 2015
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Ingår i: IEEE Photonics Technology Letters. - 1041-1135 .- 1941-0174. ; 27:17, s. 1799-1801
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Tidskriftsartikel (refereegranskat)abstract
- We demonstrate 4-PAM 48.67 Gb/s transmission, using a directly modulated VCSEL, over 50, 100 and 200 m of multimode fiber with partial dispersion compensation properties. The results of our experiments indicate that 200 m reach is achievable with forward error correcting codes with small overhead.
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| 37. |
- Castro, Jose Manuel, et al.
(författare)
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50 Gb/s 4-PAM over 200 m of High Bandwidth MMF using a 850 nm VCSEL
- 2015
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Ingår i: Optical Fiber Communication Conference 2015, Los Angeles, California, United States, 22–26 March 2015. - 9781557529374
-
Konferensbidrag (refereegranskat)abstract
- Experiments for transmission of 50Gb/s, 4-PAM using direct modulated VCSEL over 100m and 200m of MMF with partial dispersion compensation properties are presented.
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| 38. |
- Castro, J. M., et al.
(författare)
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Investigation of 60 Gb/s 4-PAM Using an 850 nm VCSEL and Multimode Fiber
- 2016
-
Ingår i: Journal of Lightwave Technology. - : Institute of Electrical and Electronics Engineers (IEEE). - 0733-8724 .- 1558-2213. ; 34:16, s. 3825-3836
-
Tidskriftsartikel (refereegranskat)abstract
- We investigate 4-pulse-amplitude modulation (PAM) at data rates in the range of 50-60 Gb/s using a directly modulated 850 nm VCSEL and multimode fiber links up to 200 m. The channel conditions in which 4-PAM has performance advantages over traditional ON-OFF keying are evaluated followed by experimental results at data rates of 56.7 Gb/s over distances up to 200 m using equalization and FEC, with a post-FEC bit error rate of 10(-12). The reported analysis is applicable to future Ethernet and Fibre Channel applications requiring 50 Gb/s serial data rates per lane over multimode fibers.
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| 39. |
- Chen, Jingjing, 1982, et al.
(författare)
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An Energy Efficient 56 Gbps PAM-4 VCSEL Transmitter Enabled by a 100 Gbps Driver in 0.25 µm InP DHBT Technology
- 2016
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Ingår i: Journal of Lightwave Technology. - 0733-8724 .- 1558-2213. ; 34:21, s. 954-4964
-
Tidskriftsartikel (refereegranskat)abstract
- Datacenters demand higher speed VCSEL-based optical interconnects at low power consumption. As a potential technology enabler, this paper presents a wide bandwidth and energy efficient multilevel PAM VCSEL driver implemented in an InP 0.25 μm double heterojunction bipolar transistor (DHBT) technology. The operational bandwidth of the driver is verified and error-free electrical data transmission up to 56 Gbps PAM-2 and 100 Gbps PAM-4 is demonstrated at a driver energy consumption less than 2 pJ/bit. The driver is integrated and tested with an in-house fabricated 850 nm VCSEL. Error-free 56 Gbps PAM-4 optical transmission at a transmitter energy consumption of 3.7 pJ/bit is demonstrated without equalization. This is the highest data rate reported for an integrated PAM-4 modulated VCSEL transmitter, while being the most energy efficient above 40 Gbps operation. Moreover, the VCSEL driver offers a pre-emphasis feature at PAM-2 operation for improved link throughput and receiver sensitivity.
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| 40. |
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| 41. |
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| 42. |
- Davani, Hooman A., et al.
(författare)
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Polarization investigation of a tunable high-speed short-wavelength bulk-micromachined MEMS-VCSEL
- 2012
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Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 0277-786X .- 1996-756X. - 9780819489197 ; 8276, s. Art. no. 82760T-
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Konferensbidrag (refereegranskat)abstract
- We report the investigation of the state of polarization (SOP) of a tunable vertical-cavity surface-emitting laser (VCSEL) operating near 850 nm with a mode-hop free single-mode tuning range of about 12 nm and an amplitude modulation bandwidth of about 5 GHz. In addition, the effect of a sub-wavelength grating on the device and its influence on the polarization stability and polarization switching has been investigated. The VCSEL with an integrated sub-wavelength grating shows a stable SOP with a polarization mode suppression ratio (PMSR) more than 35 dB during the tuning.
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| 43. |
- Davani, Hooman A., et al.
(författare)
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Widely Electro Thermal Tunable Bulk-Micromachined MEMS-VCSEL Operating Around 850nm
- 2011
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Ingår i: Conference on Lasers and Electro-Optics/Pacific Rim, CLEOPR 2011; Sydney; Australia; 28 August 2011 through 1 September 2011. - 2162-2701. - 9780977565771 ; , s. 32-34
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Konferensbidrag (refereegranskat)abstract
- We present the highest reported continues tuning range of 37 nm and fastest electro thermal tuning speed of 700 Hz achieved with tunable vertical-cavity surface-emitting lasers (VCSEL) and semiconductor DBRs at 850 nm wavelength range.
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| 44. |
- Davani, Hooman A., et al.
(författare)
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Widely tunable high-speed bulk-micromachined short-wavelength MEMS-VCSEL
- 2010
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Ingår i: Semiconductor Laser Conference (ISLC), 2010 22nd IEEE International. - 0899-9406. - 9781424456833 ; , s. 9-10
-
Konferensbidrag (refereegranskat)abstract
- We present the first results of a high-speed bulk-micromachined tunable vertical-cavity surface-emitting laser (VCSEL) operating near 850nm using a half-symmetric resonator with a movable curved microelectromechanical system (MEMS) membrane.
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| 45. |
- de Frias, Cindy M., et al.
(författare)
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Influence of COMT Gene Polymorphism on fMRI-assessed Sustained and Transient Activity during a Working Memory Task
- 2010
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Ingår i: Journal of cognitive neuroscience. - Cambridge, Mass. : MIT Press. - 0898-929X .- 1530-8898. ; 22:7, s. 1614-1622
-
Tidskriftsartikel (refereegranskat)abstract
- The catechol O-methyltransferase (COMT) gene-encoding an enzyme that is essential for the degradation of dopamine (DA) in prefrontal cortex (PFC)-contains a single nucleotide polymorphism (val/met) important for cognition. According to the tonic-phasic hypothesis, individuals carrying the low-enzyme- activity allele (met) are characterized by enhanced tonic DA activity in PFC, promoting sustained cognitive representations in working memory. Val carriers have reduced tonic but enhanced phasic dopaminergic activity in subcortical regions, enhancing cognitive flexibility. We tested the tonic-phasic DA hypothesis by dissociating sustained and transient brain activity during performance on a 2-back working memory test using mixed blocked/event-related functional magnetic resonance imaging. Participants were men recruited from a random sample of the population (the Betula study) and consisted of 11 met/met and 11 val/val carriers aged 50 to 65 years, matched on age, education, and cognitive performance. There were no differences in 2-back performance between genotype groups. Met carriers displayed a greater transient medial temporal lobe response in the updating phase of working memory, whereas val carriers showed a greater sustained PFC activation in the maintenance phase. These results support the tonic-phasic theory of DA function in elucidating the specific phenotypic influence of the COMT val(158)met polymorphism on different components of working memory.
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| 46. |
- Dussex, Nicolas, et al.
(författare)
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Integrating multi-taxon palaeogenomes and sedimentary ancient DNA to study past ecosystem dynamics
- 2021
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Ingår i: Proceedings of the Royal Society of London. Biological Sciences. - : The Royal Society. - 0962-8452 .- 1471-2954. ; 288:1957
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Forskningsöversikt (refereegranskat)abstract
- Ancient DNA (aDNA) has played a major role in our understanding of the past. Important advances in the sequencing and analysis of aDNA from a range of organisms have enabled a detailed understanding of processes such as past demography, introgression, domestication, adaptation and speciation. However, to date and with the notable exception of microbiomes and sediments, most aDNA studies have focused on single taxa or taxonomic groups, making the study of changes at the community level challenging. This is rather surprising because current sequencing and analytical approaches allow us to obtain and analyse aDNA from multiple source materials. When combined, these data can enable the simultaneous study of multiple taxa through space and time, and could thus provide a more comprehensive understanding of ecosystem-wide changes. It is therefore timely to develop an integrative approach to aDNA studies by combining data from multiple taxa and substrates. In this review, we discuss the various applications, associated challenges and future prospects of such an approach.
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| 47. |
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| 48. |
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| 49. |
- Ekenberg, Love, et al.
(författare)
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Deliberation, representation, equity : research approaches, tools and algorithms for participatory processes
- 2017
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- What can we learn about the development of public interaction in e-democracy from a drama delivered by mobile headphones to an audience standing around a shopping center in a Stockholm suburb? In democratic societies there is widespread acknowledgment of the need to incorporate citizens' input in decision-making processes in more or less structured ways. But participatory decision making is balancing on the borders of inclusion, structure, precision and accuracy. To simply enable more participation will not yield enhanced democracy, and there is a clear need for more elaborated elicitation and decision analytical tools. This rigorous and thought-provoking volume draws on a stimulating variety of international case studies, from flood risk management in the Red River Delta of Vietnam, to the consideration of alternatives to gold mining in Ro?ia Montana in Transylvania, to the application of multi-criteria decision analysis in evaluating the impact of e-learning opportunities at Uganda's Makerere University. Editors Love Ekenberg (senior research scholar, International Institute for Applied Systems Analysis [IIASA], Laxenburg, professor of Computer and Systems Sciences, Stockholm University), Karin Hansson (artist and research fellow, Department of Computer and Systems Sciences, Stockholm University), Mats Danielson (vice president and professor of Computer and Systems Sciences, Stockholm University, affiliate researcher, IIASA) and Göran Cars (professor of Societal Planning and Environment, Royal Institute of Technology, Stockholm) draw innovative collaborations between mathematics, social science, and the arts. They develop new problem formulations and solutions, with the aim of carrying decisions from agenda setting and problem awareness through to feasible courses of action by setting objectives, alternative generation, consequence assessments, and trade-off clarifications. As a result, this book is important new reading for decision makers in government, public administration and urban planning, as well as students and researchers in the fields of participatory democracy, urban planning, social policy, communication design, participatory art, decision theory, risk analysis and computer and systems sciences.
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| 50. |
- Ekholm, Marcus, et al.
(författare)
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Ab initio calculations and synthesis of the off-stoichiometric half-Heusler phase Ni1-xMn1+xSb
- 2010
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Ingår i: JOURNAL OF APPLIED PHYSICS. - : American Institute of Physics. - 0021-8979 .- 1089-7550. ; 108:9
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Tidskriftsartikel (refereegranskat)abstract
- We perform a combined theoretical and experimental study of the phase stability and magnetism of the off-stoichiometric Ni1-xMn1+xSb in the half-Heusler crystal phase. Our work is motivated by the need for strategies to engineer the magnetism of potentially half-metallic materials, such as NiMnSb, for improved performance at elevated temperatures. By means of ab initio calculations we investigate Ni1-xMn1+xSb over the whole composition range 0 andlt;= x andlt;= 1 of Ni replacing Mn and show that at relevant temperatures, the half-Heusler phase should be thermodynamically stable up to at least x=0.20 with respect to the competing C38 structure of Mn2Sb. Furthermore we find that half-Heusler Ni1-xMn1+xSb retains half-metallic band structure over the whole concentration range and that the magnetic moments of substitutional Mn-Ni atoms display magnetic exchange interactions an order of magnitude larger than the Ni-Mn interaction in NiMnSb. We also demonstrate experimentally that the alloys indeed can be created by synthesizing off-stoichiometric Ni1-xMn1+xSb films on MgO substrates by means of magnetron sputtering.
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