| 1. |
- Li, Longkun, et al.
(författare)
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Changes in T-type calcium channel and its subtypes in overactive detrusor of the rats with partial bladder outflow obstruction
- 2007
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Ingår i: Neurourology and Urodynamics. - : Wiley. - 0733-2467 .- 1520-6777. ; 26:6, s. 870-878
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Tidskriftsartikel (refereegranskat)abstract
- AimsTo investigate the activity of the T-type calcium channel (TCC) and the expression of its subtypes in overactive detrusor (OD) myocytes in rats after partial bladder outflow obstruction (PBOO).MethodsThirteen male Wistar rats with OD after PBOO (OD group) and eight sham-operated rats (control group) were studied. The two groups were compared regarding the expression of TCC subtype genes by reverse transcription-polymerase chain reaction (RT-PCR) and the TCC kinetics and cell action potential by whole-cell patch-clamp.ResultsThe time course and density of the current were significantly higher in the OD cells than those in the control detrusor. Whole-cell patch-clamp analysis showed that the activation of TCCs in detrusor myocytes in the OD group was faster than the control group, but inactivation was almost the same in both groups, suggesting a significant enhancement of the Ca2+ window current in the OD group. Patch-clamp recording of action potentials in the OD cells indicated an increase in excitability and a decrease in the repolarization interval. RT-PCR assay showed an abnormal expression of 1G subtype in the OD cells.ConclusionsTCCs could be one of the crucial factors for the abnormal excitation in OD cells. The development of OD after PBOO presumably relates to the increase in TCC current in the bladder cells, the enhancement of the Ca2+ window current for Ca2+ inflow, the prolongation of the intracellular calcium oscillations, and the acceleration of the cell depolarization.
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| 2. |
- Li, Longkun, et al.
(författare)
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Changes of gap junctional cell-cell communication in overactive detrusor in rats
- 2007
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Ingår i: American Journal of Physiology - Cell Physiology. - : American Physiological Society. - 0363-6143 .- 1522-1563. ; 293:5, s. c1627-1635
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Tidskriftsartikel (refereegranskat)abstract
- To evaluate the changes in intercellular communication through gap junctions in detrusor overactivity (DO), we studied 23 adult female Wistar rats with DO after partial outflow obstruction (DO group) and 13 sham-operated rats (control group). The two groups were compared by means of urodynamics, light and electron microscopy, expression of Cx40, Cx43, and Cx45 mRNA genes with RT-PCR, Cx43 protein with Western blot analysis, and functional intercellular communication with scrape loading dye transfer (SLDT) and fluorescence recovery after photobleaching (FRAP). The number of gap junctions and the expression of connexin mRNA and Cx43 protein were increased in DO rats, and intercellular communication through gap junctions increased after 6 wk of partial outflow obstruction as assessed with SLDT and FRAP techniques. The findings provide a theoretical rationale for using Cx43 antagonists and gap junction inhibitors in the treatment of patients with overactive detrusor secondary to partial bladder outflow obstruction.
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| 3. |
- Li, longkun, et al.
(författare)
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Transurethral endoscopy technique with a ureteroscope for diagnosis and management of seminal tracts disorders: a new approach
- 2008
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Ingår i: Journal of endourology. - : Mary Ann Liebert Inc. - 0892-7790 .- 1557-900X. ; 22:4, s. 719-724
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: To apply a transurethral endoscopic technique for examining and managing suspicious distal seminal tracts disorders with a ureteroscope. Patients and Methods: Sixteen patients with distal seminal tracts disorders underwent transurethral endoscopy through the distal seminal tracts using a semirigid ureteroscope. Of the 16 patients, 6 had suspected hemospermia, 4 spermatocele, and 6 male infertility. Results: The ejaculatory duct, seminal vesicle, and ampulla of the vas deferens were observed under direct vision with the ureteroscope. The vas deferens was investigated by cannulation with a guidewire or an epidural anesthesia catheter. Four patients received a diagnosis of spermatocele, four seminal vesiculitis, and three vas deferens obstruction. All patients received appropriate treatment. The remaining five patients had no anatomic disorders. All patients received careful postoperative observation and treatment, and were monitored for at least 3 months. Three patients had postoperative discomfort in the perineal region. There were no further complications. Conclusions: This new technique with the ureteroscope enables diagnosis and management of distal seminal tracts disorders through the normal anatomic tract. This endoscopic technique can be performed easily with minimal complications.
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| 4. |
- Li, Pan, et al.
(författare)
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Silver nano-needles : Focused optical field induced solution synthesis and application in remote-excitation nanofocusing SERS
- 2019
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Ingår i: Nanoscale. - : Royal Society of Chemistry (RSC). - 2040-3364 .- 2040-3372. ; 11:5, s. 2153-2161
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Tidskriftsartikel (refereegranskat)abstract
- Tapered metallic nanostructures that harbor surface plasmons are highly interesting for nanophotonic applications because of their waveguiding and field-focusing properties. Here, we developed a focused optical field induced solution synthesis for unique crystallized silver nano-needles. Under the focused laser spot, inhomogeneous Ag monomer concentration is created, which triggers the uniaxial growth of silver nanostructures along the radial direction with decreasing rate, forming nano-needle structures. These nano-needles are several micrometers long, with diameter attenuating from hundreds to tens of nanometers, and terminated by a sharp apex only a few nanometers in diameter. Moreover, nano-needles with atomically smooth surfaces show excellent performance for plasmonic waveguiding and unique near-field compression abilities. This nano-needle structure can be used for effective remote-excitation detection/sensing. We also demonstrate the assembling and picking up of nano-needles, which indicate potential applications in intracellular endoscopy, high resolution scanning tips, on-chip nanophotonic devices, etc.
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| 5. |
- Li, Longkun, et al.
(författare)
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Altered expression of calcium-activated K and Cl channels in detrusor overactivity of rats with partial bladder outflow obstruction
- 2008
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Ingår i: BJU International. - 1464-4096 .- 1464-410X. ; 101:12, s. 1588-1594
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Tidskriftsartikel (refereegranskat)abstract
- To evaluate the activity of large- and small-conductance calcium-activated potassium channels (BKCa, SKCa) and calcium-activated chloride channels (ClCa) in detrusor overactivity (DO) cells after partial bladder outlet obstruction (PBOO) in rats. MATERIALS AND METHODS Thirteen female Wistar rats with DO caused by PBOO were studied simultaneously with eight sham-operated rats. The expression of KCa and ClCa channels was assessed by reverse transcription-polymerase chain reaction, and the function of the two groups compared. RESULTS In the DO cells the expression of BKCa, SKCa2 and SKCa3 was lower, and that of ClCa channels higher, than in the control group cells. Using confocal laser scanning microscopic analysis, the function of BKCa and SKCa channels was suppressed, and that of ClCa channels was enhanced in DO group cells. KCa and ClCa effectors altered the cell membrane potentials more significantly in the DO cells than in the control cells, indicating a decrease in KCa and an increase in ClCa in DO group in either iso- or hypo-osmolar medium. Moreover, the change in BKCa, SKCa and ClCa channel activators in DO cells showed a more excitable state in hypo-osmolar medium than in iso-osmolar medium. CONCLUSION In DO myocytes after PBOO, the expression and function of KCa channels were decreased, and those of ClCa channels increased. These changes all provoke greater cell excitability, and could partly account for the DO.
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| 6. |
- Song, Bo, et al.
(författare)
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Newly found prostate-bladder neural reflex in rats--possible mechanism for voiding dysfunction associated with prostatitis/pelvic pain
- 2009
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Ingår i: Urology. - : Elsevier BV. - 0090-4295 .- 1527-9995. ; 74:6, s. 1365-1369
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Tidskriftsartikel (refereegranskat)abstract
- Objectives To demonstrate the existence of a prostate-bladder neural reflex that might help clarify a neurologic mechanism for voiding dysfunction associated with chronic prostatitis/chronic pelvic pain syndrome. Methods Experiments were performed on anesthetized adult male Wistar rats. Repeated cystometry was used to study the changes in urinary bladder behavior induced by injecting formalin into the prostate. The pathway of a prostate-bladder reflex was identified by recording the electromyographic (EMG) response of the detrusor to electrical stimulation of the prostate, after saline or lidocaine injections into the prostate, and transection of the prostate nerves. Results Intraprostatic formalin injection induced significant changes in the parameters of cystometry. Electrical stimulation of the prostate consistently evoked a bladder EMG response. Intraprostatic lidocaine injection increased the delay and reduced the amplitude of this EMG response. The bladder EMG response was not affected by transection of the cervical spinal cord nor by just cutting the sympathetic chain; however, it was suppressed by resection of the sympathetic chain and by transection of the ipsilateral L6-S3 nerve roots. Conclusions A prostate-bladder reflex is proposed, and the lumbosacral spinal cord is assumed to be the primary center of the reflex. These findings might help determine the therapeutic approach to voiding dysfunction in patients with chronic prostatitis/chronic pelvic pain syndrome.
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