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1.
  • Beal, Jacob, et al. (author)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • In: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Journal article (peer-reviewed)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Ablikim, M., et al. (author)
  • First measurement of e(+)e(-) -> pK(S)(0)(n)over-barK(-) + c.c. above open charm threshold
  • 2018
  • In: Physical Review D. - 2470-0010 .- 2470-0029. ; 98:3
  • Journal article (peer-reviewed)abstract
    • The process e(+)e(-) -> pK(S)(0)(n) over barK(-) + c.c. and its intermediate processes are studied for the first time, using data samples collected with the BESIII detector at BEPCII at center-of-mass energies of 3.773, 4.008, 4.226, 4.258, 4.358, 4.416, and 4.600 GeV, with a total integrated luminosity of 7.4 fb(-1). The Born cross section of e(+)e(-) -> pK(S)(0)(n) over barK(-) + c.c. is measured at each center-of-mass energy, but no significant resonant structure in the measured cross-section line shape between 3.773 and 4.600 GeV is observed. No evident structure is detected in the pK(-), nK(S)(0), pK(S)(0), nK(+), p (n) over bar, or (KSK-)-K-0 invariant mass distributions except for Lambda(1520). The Born cross sections of e(+)e(-) -> Lambda(1520)(n) over barK(S)(0) + c.c. and e(+)e(-) -> Lambda(1520)(p) over barK(+) + c.c. are measured, and the 90% confidence level upper limits on the Born cross sections of e(+)e(-) -> Lambda(1520)(Lambda) over bar (1520) are determined at the seven center-of-mass energies. There is an evident difference in line shape and magnitude of the measured cross sections between e(+)e(-) -> Lambda(1520)(-> pK(-))(n) over barK(S)(0) and e(+)e(-) -> pK-(Lambda) over bar (1520)(-> (n) over barK(S)(0)).
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3.
  • Ablikim, M., et al. (author)
  • Measurement of e(+)e(-) -> D(D)over-bar cross sections at the psi(3770) resonance
  • 2018
  • In: Chinese Physics C. - : IOP PUBLISHING LTD. - 1674-1137 .- 2058-6132. ; 42:8
  • Journal article (peer-reviewed)abstract
    • We report new measurements of the cross sections for the production of D (D) over bar final states at the psi(3770) resonance. Our data sample consists of an integrated luminosity of 2.93 fb(-1) of e(+)e(-) annihilation data produced by the BEPCII collider and collected and analyzed with the BESIII detector. We exclusively reconstruct three D-0 and six D+ hadronic decay modes and use the ratio of the yield of fully reconstructed D (D) over bar events ("double tags") to the yield of all reconstructed D or (D) over bar mesons ("single tags") to determine the number of D-0(D) over bar (0) and D+D- events, benefiting from the cancellation of many systematic uncertainties. Combining these yields with an independent determination of the integrated luminosity of the data sample, we find the cross sections to be sigma(e(+)e(-) -> D-0(D) over bar (0)(-) )=(3.615 +/- 0.010 +/- 0.038) nb and sigma(e(+)e(-) -> D+D-)=(2.830 +/- 0.011 +/- 0.026) nb, where the uncertainties are statistical and systematic, respectively.
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4.
  • Ablikim, M., et al. (author)
  • Measurement of e(+)e(-) -> K(K)over-barJ/psi cross sections at center-of-mass energies from 4.189 to 4.600 GeV
  • 2018
  • In: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:7
  • Journal article (peer-reviewed)abstract
    • We investigate the process e(+)e(-) -> K (K) over barJ/psi at center-of-mass energies from 4.189 to 4.600 GeV using 4.7 fb(-1) of data collected by the BESIII detector at the BEPCII collider. The Born cross sections for the reactions e(+)e(-) -> K(+)K(-)J/psi and K(S)(0)K(S)(0)J/psi are measured as a function of center-of-mass energy. The energy dependence of the cross section for e(+)e(-) -> K(+)K(-)J/psi is shown to differ from that for pi(+)pi(-)J/psi in the region around the Y(4260). In addition, there is evidence for a structure around 4.5 GeV in the e(+)e(-) -> K(+)K(-)J/psi cross section that is not present in pi(+)pi(-)J/psi.
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5.
  • Ablikim, M., et al. (author)
  • Measurement of the absolute branching fraction of D*(s0) (2317)(+/-) -> pi D-0(s)+/-
  • 2018
  • In: Physical Review D. - : American Physical Society. - 2470-0010 .- 2470-0029. ; 97:5
  • Journal article (peer-reviewed)abstract
    • The process e(+)e(-) -> D*D-+(s)*(s0) (2317)(-) + c.c. is observed for the first time with the data sample of 567 pb(-1) collected with the BESIII detector operating at the BEPCII collider at a center-of-mass energy root s = 4.6 GeV. The statistical significance of the D*(s0) (2317)(+/-) signal is 5.8 sigma and the mass is measured to be (2318.3 +/- 1.2 +/- 1.2) MeV/c(2). The absolute branching fraction B(D*(s0) (2317)(+/-) -> pi D-0(s)+/-) is measured as 1.00(-0.14)(+0.00) (stat)(-0.14)(+0.00) (syst) for the first time. The uncertainties are statistical and systematic, respectively.
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6.
  • Ablikim, M., et al. (author)
  • Observation of psi(3686) -> eta ' e(+)e(-)
  • 2018
  • In: Physics Letters B. - : ELSEVIER SCIENCE BV. - 0370-2693 .- 1873-2445. ; 783, s. 452-458
  • Journal article (peer-reviewed)abstract
    • Using a data sample of 448.1 x 10(6) psi(3686) events collected with the BESIII detector at the BEPCII collider, we report the first observation of the electromagnetic Dalitz decay psi(3686) -> eta'e(+)e(-), with significances of 7.0 sigma and 6.3 sigma when reconstructing the eta' meson via its decay modes eta' -> gamma pi(+)pi(-) and eta' -> pi(+)pi(-) eta (eta -> gamma gamma), respectively. The weighted average branching fraction is determined to be B(psi(3686) -> eta'e(+)e(-)) = (1.90 +/- 0.25 +/- 0.11) x 10(-6), where the first uncertainty is statistical and the second systematic.
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7.
  • Ablikim, M., et al. (author)
  • Observation of the Semileptonic Decay D-0 -> a(0)(980)(-)e(+)nu(e) and Evidence for D+ -> a(0)(980)(0)e(+)nu(e)
  • 2018
  • In: Physical Review Letters. - : AMER PHYSICAL SOC. - 0031-9007 .- 1079-7114. ; 121:8
  • Journal article (peer-reviewed)abstract
    • Using an e(+)e(-) collision data sample of 2.93 fb(-1) collected at a center-of-mass energy of 3.773 GeV by the BESIII detector at BEPCII, we report the observation of D-0 -> a(0)(980)(-)e(+)nu(e) and evidence for D+ -> a(0)(980)(0)e(+)nu(e) with significances of 6.4 sigma and 2.9 sigma, respectively. The absolute branching fractions are determined to be B(D-0 -> a(0)(980)(-)e(+)nu(e)) x B(a(0)(980)(-) -> eta pi(-)) = [1.33(-0.29)(+0.33)(stat) +/- 0.09(syst)] x 10(-4) and B(D+ -> a(0)(980)(0)e(+)nu(e)) x B(a(0)(980)(0) -> eta pi(0)) = [1.66(-0.66)(+0.81)(stat) +/- 0.11(syst) x 10(-4). This is the first time the a(0)(980) meson has been measured in a D-0 semileptonic decay, which would open one more interesting page in the investigation of the nature of the puzzling a(0)(980) states.
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8.
  • Ablikim, M., et al. (author)
  • Precision Measurement of the e(+)e(-) -> Lambda(+)(c)(Lambda)over-bar(c)(-) Cross Section Near Threshold
  • 2018
  • In: Physical Review Letters. - : American Physical Society. - 0031-9007 .- 1079-7114. ; 120:13
  • Journal article (peer-reviewed)abstract
    • The cross section of the e(+)e(-) -> Lambda(+)(c)(Lambda) over bar (-)(c) process is measured with unprecedented precision using data collected with the BESIII detector at root s = 4574.5, 4580.0, 4590.0 and 4599.5 MeV. The nonzero cross section near the Lambda(+)(c)(Lambda) over bar (-)(c) production threshold is cleared. At center-of-mass energies root s = 4574.5 and 4599.5 MeV, the higher statistics data enable us to measure the Lambda(c) polar angle distributions. From these, the.c electric over magnetic form-factor ratios (vertical bar G(E)/G(M)vertical bar) are measured for the first time. They are found to be 1.14 +/- 0.14 +/- 0.07 and 1.23 +/- 0.05 +/- 0.03, respectively, where the first uncertainties are statistical and the second are systematic.
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9.
  • Ablikim, M., et al. (author)
  • Search for invisible decays of omega and phi with J/psi data at BESIII
  • 2018
  • In: Physical Review D. - : American Physical Society. - 2470-0010 .- 2470-0029. ; 98:3
  • Journal article (peer-reviewed)abstract
    • Using a data sample of (1310.6 +/- 7.0) x 10(6) J/psi events collected with the BESIII detector operating at the BEPCII collider, we perform the first experimental search for invisible decays of a light vector meson (V = omega, phi) via J/psi -> V-eta decays. The decay of eta -> pi(+)pi(-)pi(0) is utilized to tag the V meson decaying into the invisible final state. No evidence for a significant invisible signal is observed, and the upper limits on the ratio of branching fractions at the 90% confidence level are determined to be B(omega -> invisible)/B(omega -> pi(+)pi(-)pi(0)) < 8.1 x 10(-5) and B(phi -> invisible)/B(phi -> K+K-) < 3.4 x 10(-4). By using the world average values of B(omega -> pi(+)pi(-)pi(0) and B(phi -> K+K-,) the upper limits on the decay branching fractions at the 90% confidence level are set as B(omega -> invisible) < 7.3 x 10(-5) and B(phi -> invisible) < 1.7 x 10(-4), respectively.
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10.
  • Ablikim, M., et al. (author)
  • Search for the rare decay of ψ(3686)→Λ+c¯pe+e−+c.c. at BESIII
  • 2018
  • In: Physical Review D. - 2470-0010 .- 2470-0029. ; 97:9
  • Journal article (peer-reviewed)abstract
    • Based on a data sample of (448.1 +/- 2.9) x 10(6)Psi(3686) decays collected with the BESIII experiment, a search for the flavor changing neutral current transition Psi(3686) -> Lambda(+)(c) pe(+) e(-) + c.c. is performed for the first time. No signal candidates are observed and the upper limit on the branching fraction of Psi(3686) -> Lambda(+)(c) pe(+) e(-) is determined to be 1.7 x 10(-6) at the 90% confidence level. The result is consistent with expectations from the standard model, and no evidence for new physics is found.
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11.
  • Ablikim, M., et al. (author)
  • Search for the rare decays D -> h(h((')))e(+) e(-)
  • 2018
  • In: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:7
  • Journal article (peer-reviewed)abstract
    • We search for rare decays of D mesons to hadrons accompanied by an electron-positron pair (h(h((')))e(+)e(- )),using an e(+)e(-) collision sample corresponding to an integrated luminosity of 2.93 fb(-1) collected with the BESIII detector at root s = 3.773 GeV. No significant signals are observed, and the corresponding upper limits on the branching fractions at the 90% confidence level are determined. The sensitivities of the results are at the level of 10(-5)-10(-6), providing a large improvement over previous searches.
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12.
  • Ablikim, M., et al. (author)
  • Amplitude analysis of the KSKS system produced in radiative J /psi decays
  • 2018
  • In: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 98:7
  • Journal article (peer-reviewed)abstract
    • An amplitude analysis of the KSKS system produced in radiative J/psi decays is performed using the (1310.6 +/- 7.0) x 10(6) nip decays collected by the BESIII detector. Two approaches are presented. A mass-dependent analysis is performed by parametrizing the KSKS invariant mass spectrum as a sum of Breit-aligner line shapes. Additionally, a mass-independent analysis is performed to extract a piecewise function that describes the dynamics of the KSKS system while making minimal assumptions about the properties and number of poles in the amplitude. The dominant amplitudes in the mass-dependent analysis include the f(0)(1710), f(0)(2200), and f(2)'(1525). The mass-independent results, which are made available as input for further studies, are consistent with those of the mass-dependent analysis and are useful for a systematic study of hadronic interactions. The branching fraction of radiative J/psi decays to KSKS is measured to be (8.1 +/- 0.4) x 10(-4), where the uncertainty is systematic and the statistical uncertainty is negligible.
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13.
  • Ablikim, M., et al. (author)
  • Measurement of the Absolute Branching Fraction of the Inclusive Decay Lambda(+)(c) -> Lambda plus X
  • 2018
  • In: Physical Review Letters. - : AMER PHYSICAL SOC. - 0031-9007 .- 1079-7114. ; 121:6
  • Journal article (peer-reviewed)abstract
    • Based on an e(+)e(-) collision data sample corresponding to an integrated luminosity of 567 pb(-1) taken at the center-of-mass energy of root s = 4.6 GeV with the BESIII detector, we measure the absolute branching fraction of the inclusive decay Lambda(+)(c) -> Lambda + X to be B(Lambda(+)(c) -> Lambda + X) = (38.2(-2.2)(+2.8) +/- 0.9)% using the double-tag method, where X refers to any possible final state particles. In addition, we search for direct CP violation in the charge asymmetry of this inclusive decay for the first time, and obtain A(CP) [B(Lambda(+)(c) -> Lambda + X) - B((Lambda) over bar (-)(c) -> (Lambda) over bar + X)]/[B(Lambda(+)(c) -> Lambda + X) + B((Lambda) over bar (-)(c) -> (Lambda) over bar + X)] = (2.1(-6.6)(+7.0) +/- 1.6)%, a statistically limited result with no evidence of CP violation.
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14.
  • Ablikim, M., et al. (author)
  • Measurements of the branching fractions of the singly Cabibbo-suppressed decays D0→ωη, η(')π0 and η(')η
  • 2018
  • In: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:5
  • Journal article (peer-reviewed)abstract
    • By analyzing a data sample of 2.93 fb(-1) collected at root s = 3.773 GeV with the BESIII detector operated at the BEPCII storage rings, we measure the branching fractions B(D-0 -> omega eta) = (2.15 +/- 0.17(stat) +/- 0.15(sys)) x 10(-3), B(D-0 ->eta pi(0)) = (0.58 +/- 0.05(stat) +/- 0.05(sys)) x 10(-3), B(D-0 ->eta'pi(0)) = (0.93 +/- 0.11(stat) +/- 0.09(sys)) x 10(-3), B(D-0 -> eta eta) = (2.20 +/- 0.07(stat) +/- 0.06(sys)) x 10(-3) and B(D-0 -> eta'eta) = (0.94 degrees +/- 0.25(stat) +/- 0.11(sys)) x 10(-3). We note that B(D-0 -> omega eta) is measured for the first time and that B(D-0 -> eta eta) is measured with much improved precision.
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15.
  • Ablikim, M., et al. (author)
  • Study of the decays D+-> eta(('))e(+)nu(e)
  • 2018
  • In: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:9
  • Journal article (peer-reviewed)abstract
    • The charm semileptonic decays D+ -> eta e(+)nu(e) and D+ -> eta'e(+)nu(e) are studied with a sample of e(+)e(-) collision data corresponding to an integrated luminosity of 2.93 fb(-1) collected at root s = 3.773 GeV with the BESIII detector. We measure the branching fractions for D+ -> eta e(+)upsilon(e) to be (10.74 +/- 0.81 +/- 0.51)x10(-4), and for D+ -> eta'e(+)nu(e) to be (1.91 +/- 0.51 +/- 0.13) x 10(-4), where the uncertainties are statistical and systematic, respectively. In addition, we perform a measurement of the form factor in the decay D+ -> eta e(+)nu(e) . All the results are consistent with those obtained by the CLEO-c experiment.
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16.
  • Kristan, Matej, et al. (author)
  • The Sixth Visual Object Tracking VOT2018 Challenge Results
  • 2019
  • In: Computer Vision – ECCV 2018 Workshops. - Cham : Springer Publishing Company. - 9783030110086 - 9783030110093 ; , s. 3-53
  • Conference paper (peer-reviewed)abstract
    • The Visual Object Tracking challenge VOT2018 is the sixth annual tracker benchmarking activity organized by the VOT initiative. Results of over eighty trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in the recent years. The evaluation included the standard VOT and other popular methodologies for short-term tracking analysis and a “real-time” experiment simulating a situation where a tracker processes images as if provided by a continuously running sensor. A long-term tracking subchallenge has been introduced to the set of standard VOT sub-challenges. The new subchallenge focuses on long-term tracking properties, namely coping with target disappearance and reappearance. A new dataset has been compiled and a performance evaluation methodology that focuses on long-term tracking capabilities has been adopted. The VOT toolkit has been updated to support both standard short-term and the new long-term tracking subchallenges. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The dataset, the evaluation kit and the results are publicly available at the challenge website (http://votchallenge.net).
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17.
  • Kristan, Matej, et al. (author)
  • The Visual Object Tracking VOT2016 Challenge Results
  • 2016
  • In: COMPUTER VISION - ECCV 2016 WORKSHOPS, PT II. - Cham : SPRINGER INT PUBLISHING AG. - 9783319488813 - 9783319488806 ; , s. 777-823
  • Conference paper (peer-reviewed)abstract
    • The Visual Object Tracking challenge VOT2016 aims at comparing short-term single-object visual trackers that do not apply pre-learned models of object appearance. Results of 70 trackers are presented, with a large number of trackers being published at major computer vision conferences and journals in the recent years. The number of tested state-of-the-art trackers makes the VOT 2016 the largest and most challenging benchmark on short-term tracking to date. For each participating tracker, a short description is provided in the Appendix. The VOT2016 goes beyond its predecessors by (i) introducing a new semi-automatic ground truth bounding box annotation methodology and (ii) extending the evaluation system with the no-reset experiment.
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18.
  • Kristan, Matej, et al. (author)
  • The Visual Object Tracking VOT2017 challenge results
  • 2017
  • In: 2017 IEEE INTERNATIONAL CONFERENCE ON COMPUTER VISION WORKSHOPS (ICCVW 2017). - : IEEE. - 9781538610343 ; , s. 1949-1972
  • Conference paper (peer-reviewed)abstract
    • The Visual Object Tracking challenge VOT2017 is the fifth annual tracker benchmarking activity organized by the VOT initiative. Results of 51 trackers are presented; many are state-of-the-art published at major computer vision conferences or journals in recent years. The evaluation included the standard VOT and other popular methodologies and a new "real-time" experiment simulating a situation where a tracker processes images as if provided by a continuously running sensor. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The VOT2017 goes beyond its predecessors by (i) improving the VOT public dataset and introducing a separate VOT2017 sequestered dataset, (ii) introducing a realtime tracking experiment and (iii) releasing a redesigned toolkit that supports complex experiments. The dataset, the evaluation kit and the results are publicly available at the challenge website(1).
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19.
  • Attié, David, et al. (author)
  • A time projection chamber with GEM-based readout
  • 2017
  • In: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. - : Elsevier BV. - 0168-9002. ; 856, s. 109-118
  • Journal article (peer-reviewed)abstract
    • For the International Large Detector concept at the planned International Linear Collider, the use of time projection chambers (TPC) with micro-pattern gas detector readout as the main tracking detector is investigated. In this paper, results from a prototype TPC, placed in a 1. T solenoidal field and read out with three independent Gas Electron Multiplier (GEM) based readout modules, are reported. The TPC was exposed to a 6. GeV electron beam at the DESY II synchrotron. The efficiency for reconstructing hits, the measurement of the drift velocity, the space point resolution and the control of field inhomogeneities are presented.
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20.
  • Li, Shuting, et al. (author)
  • Direct structure determination of vemurafenib polymorphism from compact spherulites using 3D electron diffraction
  • 2023
  • In: Communications Chemistry. - : Springer Science and Business Media LLC. - 2399-3669. ; 6
  • Journal article (peer-reviewed)abstract
    • The spherulitic morphology is considered to be the most common morphology of crystalline materials and is particularly apparent in melt-crystallized products. Yet, historically, the polycrystalline nature of spherulites has hindered successful crystal structure determination. Here, we report the direct structure determination of a clinical drug, vemurafenib (VMN), in compact spherulite form using 3D electron diffraction (3D ED). VMN has four known polymorphs. We first solved the crystal structures of α-, β-, and γ-VMN from compact spherulites using 3D ED, and the resulting structures were highly consistent with those obtained by single-crystal X-ray diffraction. We then determined the crystal structure of δ-VMN—the least stable polymorph which cannot be cultivated as a single crystal—directly from the compact spherulite sample. We unexpectedly discovered a new polymorph during our studies, denoted as ε-VMN. Single crystals of ε-VMN are extremely thin and not suitable for study by X-ray diffraction. Again, we determined the structure of ε-VMN in a compact spherulite form. This successful structure elucidation of all five VMN polymorphs demonstrates the possibility of directly determining structures from melt-grown compact spherulite samples. Thereby, this discovery will improve the efficiency and broaden the scope of polymorphism research, especially within the field of melt crystallization.
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21.
  • Sun, Yasong, et al. (author)
  • Angular-spatial upwind element differential method for radiative heat transfer in a concentric spherical participating medium
  • 2022
  • In: Engineering analysis with boundary elements. - : ELSEVIER SCI LTD. - 0955-7997 .- 1873-197X. ; 144, s. 19-30
  • Journal article (peer-reviewed)abstract
    • Radiative intensity with high directional and spatial resolutions can provide abundant useful information for combustion diagnosis systems based on radiative images. In this paper, an angular-spatial upwind element differential method (ASUEDM) is developed to discretize angular direction and spatial domain of radiative transfer equation (RTE) in a spherically participating medium. Because of the strong convection characteristic of radiative transfer equation, an upwind scheme is adopted to suppress the numerical oscillation. Meanwhile, Chebyshev-Gauss-Lobatto nodes are used to minimize the effect of the Runge phenomenon. Unlike conductive or convective boundary conditions, radiative boundary condition is unidirectional boundary condition, and a singularity node exists at the boundary. To deal with this singularity, we propose a discontinuous strategy. Three examples of radiative heat transfer in concentric spheres are chosen to test the capability of ASUEDM. Compared with benchmark solutions, ASUEDM can provide higher accuracy than discrete ordinates method or finite volume method. Besides, ASUEDM can flexibly provide hp convergence rate and achieve high-resolution characterization in angular direction and spatial domain.
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22.
  • Xu, Yue, et al. (author)
  • Pyrrolylmethylene Appended Corrorin: Peripheral Coordination and Transformation to Pyridyl Incorporated Hemiporphycene Analogue
  • 2023
  • In: Organic Letters. - : AMER CHEMICAL SOC. - 1523-7060 .- 1523-7052. ; 25:10, s. 1793-1798
  • Journal article (peer-reviewed)abstract
    • A pyrrolylmethylene appended corrorin 1 was synthesized and coordinated with [Rh(CO)2Cl]2 to afford 1-Rh with unique RhI-eta 2-CC bonding in addition to the coordination of the dipyrrin-like unit and a carbonyl ligand. Further oxidation of 1 afforded 2, exhibiting a hydrocorrorinone core, and it can be further transformed into pyrrolo[3,2c]pyridine incorporated hemiporphycene analogue 3 upon treatment with HOAc. The side chain modifies the reactivity of corrorin and effectively tunes the NIR absorption of the resulting porphyrinoids.
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23.
  • You, Fang, et al. (author)
  • Interface Color Design of Intelligent Vehicle Central Console
  • 2021
  • In: Advances in Usability, User Experience, Wearable and Assistive Technology. - Cham : Springer Nature. - 9783030800901 - 9783030800918 ; , s. 784-792
  • Conference paper (peer-reviewed)abstract
    • The development of intelligent automobiles puts forward higher requirements for HMI design. The information obtained by drivers through vision accounts for 80%. As a key part of visual perception, color affects driving performance. This article investigates vehicle interface design about icon-background contrast. Three colors commonly used in car-machine interface are chosen: red, green and blue, and contrast is divided into five levels under daytime and nighttime. The participants tested three colors and the five contrasts in an experiment using classic paradigm of secondary tasks. We collected data from their driving behavior and subjective experience, trying to find a most appropriate value. The results show that recommended contrast values under different lighting conditions are 3:1 to 4:1 for red, blue, and green during the day; 3:1 for red at night, and 5:1 for blue and green, so that the vehicle central console color design references are formed.
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24.
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25.
  • Bai, Yushi, et al. (author)
  • Protein nanoribbons template enamel mineralization
  • 2020
  • In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 117:32, s. 19201-19208
  • Journal article (peer-reviewed)abstract
    • As the hardest tissue formed by vertebrates, enamel represents nature's engineering masterpiece with complex organizations of fibrous apatite crystals at the nanometer scale. Supramolecular assemblies of enamel matrix proteins (EMPs) play a key role as the structural scaffolds for regulating mineral morphology during enamel development. However, to achieve maximum tissue hardness, most organic content in enamel is digested and removed at the maturation stage, and thus knowledge of a structural protein template that could guide enamel mineralization is limited at this date. Herein, by examining a gene-modified mouse that lacked enzymatic degradation of EMPs, we demonstrate the presence of protein nanoribbons as the structural scaffolds in developing enamel matrix. Using in vitro mineralization assays we showed that both recombinant and enamel-tissue-based amelogenin nanoribbons are capable of guiding fibrous apatite nanocrystal formation. In accordance with our understanding of the natural process of enamel formation, templated crystal growth was achieved by interaction of amelogenin scaffolds with acidic macromolecules that facilitate the formation of an amorphous calcium phosphate precursor which gradually transforms into oriented apatite fibers along the protein nanoribbons. Furthermore, this study elucidated that matrix metalloproteinase-20 is a critical regulator of the enamel mineralization as only a recombinant analog of a MMP20-cleavage product of amelogenin was capable of guiding apatite mineralization. This study highlights that supramolecular assembly of the scaffold protein, its enzymatic processing, and its ability to interact with acidic carrier proteins are critical steps for proper enamel development.
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26.
  • Du, Gaoming, et al. (author)
  • SSS : Self-aware System-on-chip Using a Static-dynamic Hybrid Method
  • 2019
  • In: ACM Journal on Emerging Technologies in Computing Systems. - : ASSOC COMPUTING MACHINERY. - 1550-4832 .- 1550-4840. ; 15:3
  • Journal article (peer-reviewed)abstract
    • Network-on-Chip (NoC) has become the de facto communication standard for multi-core or many-core System-on-Chip (SoC) due to its scalability and flexibility. However, an important factor in NoC design is temperature, which affects the overall performance of SoC-decreasing circuit frequency, increasing energy consumption, and even shortening chip lifetime. In this article, we propose SSS, a self-aware SoC using a static-dynamic hybrid method that combines dynamic mapping and static mapping to reduce the hotspot temperature for NoC-based SoCs. First, we propose monitoring and thermal modeling for self-state sensoring. Then, in static mapping stage, we calculate the optimal mapping solutions under different temperature modes using the discrete firefly algorithm to help self-decisionmaking. Finally, in dynamic mapping stage, we achieve dynamic mapping through configuring NoC and SoC sentient units for self-optimizing. Experimental results show that SSS has substantially reduced the peak temperature by up to 37.52%. The FPGA prototype proves the effectiveness and smartness of SSS in reducing hotspot temperature.
  •  
27.
  • Hu, Guojie, et al. (author)
  • Water and heat coupling processes and its simulation in frozen soils : Current status and future research directions
  • 2023
  • In: Catena. - : Elsevier BV. - 0341-8162. ; 222
  • Research review (peer-reviewed)abstract
    • To date, most studies on coupled-water-and-heat processes in frozen soils haves focused on the mechanism of changes in frozen soil and the contribution of climate change, hydrological processes, and ecosystems in cold regions. Several studies have demonstrated considerable improvements in the accuracy of simulating water and heat transfer processes in cold regions. However, substantial differences remain among the different models and parameterizations because of the lack of observations and in-depth understanding of the water and heat transfer processes. Hence, it is necessary to summarize recent advances in the simulation of water-and-heat-coupling processes and challenges for further research. Therefore, we present a theory-focused summary of progress in this field considering the aspects of water flow and coupled-water-and-heat transfer. The simulation progress is discussed in terms of physical process models; one type of model only considers the heat conduction transfer processes without water flow, and the other considers coupled-water-and-heat transfer processes. Aspects of model deficiencies related to non-conductive heat transfer and soil water transfer processes in the frozen soil are also summarized. Moreover, the major parameterizations are reviewed, including phase changes, freeze–thaw fronts, thermal conductivity, hydraulic conductivity, snow processes, surface parameterization schemes, ground ice, and lower boundary conditions. While models and parameterizations can suitably capture local-scale water and heat transfer processes in frozen soil, their applications are spatiotemporally constrained, requiring further improvement. We provide a theoretical basis for further studying water and heat transfer processes in frozen soil and suggest that future research should enhance the accuracy of frozen soil parameterization and improve the understanding of the coupling of water and heat transfer processes based on improved observation techniques and high-resolution data.
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28.
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29.
  • Huang, Xin, 1982-, et al. (author)
  • Privacy of Value-Added Context-Aware Service Cloud
  • 2009
  • In: Lecture notes in computer science Volume 5931. - Berlin, Heidelberg : Springer. - 9783642106644 ; , s. 547-552
  • Conference paper (peer-reviewed)abstract
    • In the cloud computing era, service provider cloud and context service cloud store all your personal context data. This is a positive aspect for value-added context-aware service cloud as it makes that context information collection are easier than was the case previously. However, this computing environment does add a series of threats in relation to privacy protection. Whoever receives the context information is able to deduce the status of the owners and, generally owners are not happy to share this information. In this paper, we propose a privacy preserved framework which can be utilized by value-added context-aware service cloud. Context data and related services access privileges are determined by context-aware role-based access control (CRAC) extended from role-based access control (RAC). Privacy preserved context service protocol (PPCS) is designed to protect user privacy from exposed context information. Additionally, user network and information diffusion is combined to evaluate the privacy protection effect.
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30.
  • Li, Xinmin, et al. (author)
  • Molecular dynamics study on friction of the iron-aluminum alloy
  • 2022
  • In: Materials Today Communications. - : Elsevier BV. - 2352-4928. ; 33, s. 104402-
  • Journal article (peer-reviewed)abstract
    • Iron-aluminum alloy has been widely used in aerospace, nuclear power, electronics and other fields. Taking into account the limitations of the continuum mechanics model at the microscopic scale, it is critical to use nanoscale simulation methods like molecular dynamics (MD) to have a deeper understanding of the friction and wear behavior. Through MD simulation, this thesis mainly studied the friction and wear process of iron aluminum alloy under different conditions. The main conclusions are as follows:(1) The frictional force first experiences a period of growth, and then stabilizes with some fluctuations due to the release of stress-energy caused by deformation.(2) Temperature and friction speed has no significant effect on friction force.(3) The higher the friction speed is, the more wear debris it produces, and the more severely the material is damaged.(4) The force and coefficient of friction will increase with the rise of aluminum content, and the plastic deformation of the alloy is strengthened, while the elastic deformation decreases.(5) The frictional force increases with the wear depth since the accumulation phenomenon of atoms becomes more obvious.
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31.
  • Liu, Yehai, et al. (author)
  • Prognostic significance of matrix metalloproteinase-20 overexpression in laryngeal squamous cell carcinoma
  • 2011
  • In: Acta Oto-Laryngologica. - : Informa UK Limited. - 0001-6489 .- 1651-2251. ; 131:7, s. 769-773
  • Journal article (peer-reviewed)abstract
    • Conclusion: Matrix metalloproteinase (MMP)-20 is overexpressed in laryngeal squamous cell carcinoma (LSCC) compared with the adjacent normal laryngeal epithelium and MMP-20 plays a role in lymph node metastasis. Overexpression of MMP-20 may be used as a significant prognostic factor for lymph node metastasis. All the findings indicate that MMP-20 may play a role in the initiation and progression of LSCC. Objective: The MMPs are a gene family of zinc-dependent endopeptidases that have been implicated in tumor invasion and metastasis, and MMP-20 is a new member of the MMP family. The purpose of this study was to investigate whether MMP-20 is overexpressed in human LSCC and, if so, the significance of its overexpression in relation to clinical parameters. Methods: We analyzed 33 cases of LSCC with RT-PCR and 73 cases of LSCC with immunohistochemistry compared with normal laryngeal epithelium. Results: We found that MMP-20 is overexpresssed in LSCC compared with the adjacent normal laryngeal epithelium.
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32.
  • Mu, Liwen, et al. (author)
  • Molecular Origin of Efficient Phonon Transfer in Modulated Polymer Blends : Effect of Hydrogen Bonding on Polymer Coil Size and Assembled Microstructure
  • 2017
  • In: The Journal of Physical Chemistry C. - : American Chemical Society (ACS). - 1932-7447 .- 1932-7455. ; 121:26, s. 14204-14212
  • Journal article (peer-reviewed)abstract
    • Molecular level engineering of polymer or polymer blends has been recently demonstrated effective strategy to regulate thermal conductivity. Such materials are of great interest to meet critical requirements of transparent, light weight, flexible, etc for thermal management in electronic applications. In this work, modulated polymer blends with poly(vinyl alcohol) (PVA) and biopolymers (lignin, gelatin) were designed and significantly enhanced thermal conductivity was achieved by tuning the intermolecular interaction among polymer components. The hydrogen bond interaction has been revealed as the major driving force that affects the polymer coil dimension in aqueous solution, the microstructure of coil-coil interaction in solid film and thus the thermal conduction. A solid relationship across molecular level interaction to macro-scale thermal conduction is constructed via careful characterization of the coil size in liquid phase and assembled microstructure in solid phase. Appropriate integration of biopolymers and PVA is essential to achieve synergistic effect. Specifically, thermal conductivity of polymer blend with 10% lignin and 10% G90 in PVA reaches 0.71 W/m·K, which is 184% enhancement as compared to pure PVA. This work reveals the fundamental molecular origin of polymer blends in association with thermal conductivity and has great potential to guide molecular engineering for superior physicochemical properties.
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33.
  • Pang, Xiaoying, et al. (author)
  • The Present-day Mass Function of Star Clusters in the Solar Neighborhood
  • 2024
  • In: Astrophysical Journal. - 0004-637X .- 1538-4357. ; 966:2
  • Journal article (peer-reviewed)abstract
    • This work analyzes the present-day mass function (PDMF) of 93 star clusters utilizing Gaia Data Release 3 data, with membership determined by the StarGo machine-learning algorithm. The impact of unresolved binary systems on mass estimation is rigorously assessed, adopting three mass ratio profiles for correction. The PDMF is characterized by the power-law index, α, derived through a robust maximum likelihood method that avoids biases associated with data binning. The value of α for stars between the completeness limited mass of Gaia (with a mean 0.3 M⊙ for our cluster samples) and 2 M⊙ exhibits stability for clusters younger than 200 Myr, decreasing for older clusters, particularly when considering stars within the half-mass radius. The PDMF of these star clusters is consistent with a dynamically evolved Kroupa initial mass function via the loss of low-mass stars. Cluster morphology shows a correlation with α, as α values exhibit a decreasing trend from filamentary to tidal-tail clusters, mirroring the sequence of increasing cluster age. The dependence of α on the total cluster mass is weak, with a subtle increase for higher-mass clusters, especially outside the half-mass radius. We do not observe a correlation between α and the mean metallicity of the clusters. Younger clusters have lower metallicity compared to their older counterparts, which indicates that the older clusters might have migrated to the solar neighborhood from the inner disk. A comparison with numerical models incorporating a black hole population suggests the need for observations of distant, older, massive open clusters to determine whether or not they contain black holes.
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34.
  • Wienecke, Jacob, et al. (author)
  • Spinal Cord Injury Enables Aromatic l-Amino Acid Decarboxylase Cells to Synthesize Monoamines.
  • 2014
  • In: The Journal of Neuroscience. - 1529-2401. ; 34:36, s. 11984-12000
  • Journal article (peer-reviewed)abstract
    • Serotonin (5-HT), an important modulator of both sensory and motor functions in the mammalian spinal cord, originates mainly in the raphe nuclei of the brainstem. However, following complete transection of the spinal cord, small amounts of 5-HT remain detectable below the lesion. It has been suggested, but not proven, that this residual 5-HT is produced by intraspinal 5-HT neurons. Here, we show by immunohistochemical techniques that cells containing the enzyme aromatic l-amino acid decarboxylase (AADC) occur not only near the central canal, as reported by others, but also in the intermediate zone and dorsal horn of the spinal gray matter. We show that, following complete transection of the rat spinal cord at S2 level, AADC cells distal to the lesion acquire the ability to produce 5-HT from its immediate precursor, 5-hydroxytryptophan. Our results indicate that this phenotypic change in spinal AADC cells is initiated by the loss of descending 5-HT projections due to spinal cord injury (SCI). By in vivo and in vitro electrophysiology, we show that 5-HT produced by AADC cells increases the excitability of spinal motoneurons. The phenotypic change in AADC cells appears to result from a loss of inhibition by descending 5-HT neurons and to be mediated by 5-HT1B receptors expressed by AADC cells. These findings indicate that AADC cells are a potential source of 5-HT at spinal levels below an SCI. The production of 5-HT by AADC cells, together with an upregulation of 5-HT2 receptors, offers a partial explanation of hyperreflexia below a chronic SCI.
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35.
  • Wu, Hongwei, et al. (author)
  • Crystal Multi-Conformational Control Through Deformable Carbon-Sulfur Bond for Singlet-Triplet Emissive Tuning
  • 2019
  • In: Angewandte Chemie International Edition. - : WILEY-V C H VERLAG GMBH. - 1433-7851 .- 1521-3773. ; 58:13, s. 4328-4333
  • Journal article (peer-reviewed)abstract
    • Crystal-state luminophores have been of great interest in optoelectronics for years, whereas the excited state regulation at the crystal level is still restricted by the lack of control ways. We report that the singlet-triplet emissive property can be profoundly regulated by crystal conformational distortions. Employing fluoro-substituted tetrakis(arylthio)benzene luminophores as prototype, we found that couples of molecular conformations formed during different crystallizations. The deformable carbon-sulphur bond essentially drove the distortion of the molecular conformation and varied the stacking mode, together with diverse non-covalent interactions, leading to the proportional adjustment of the fluorescence and phosphorescence bands. This intrinsic strategy was further applied for solid-state multicolor emissive conversion and mechanoluminescence, probably offering new insights for design of smart crystal luminescent materials.
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36.
  • Yang, Bin, et al. (author)
  • Computer Vision and Machine Learning Methods for Heat Transfer and Fluid Flow in Complex Structural Microchannels : A Review
  • 2023
  • In: Energies. - : MDPI. - 1996-1073. ; 16:3
  • Research review (peer-reviewed)abstract
    • Heat dissipation in high-heat flux micro-devices has become a pressing issue. One of the most effective methods for removing the high heat load of micro-devices is boiling heat transfer in microchannels. A novel approach to flow pattern and heat transfer recognition in microchannels is provided by the combination of image and machine learning techniques. The support vector machine method in texture characteristics successfully recognizes flow patterns. To determine the bubble dynamics behavior and flow pattern in the micro-device, image features are combined with machine learning algorithms and applied in the recognition of boiling flow patterns. As a result, the relationship between flow pattern evolution and boiling heat transfer is established, and the mechanism of boiling heat transfer is revealed.
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37.
  • Yin, Shi, et al. (author)
  • Experimental investigation of near-field stream-wise flow development and spatial structure in triple buoyant plumes
  • 2019
  • In: Building and Environment. - : Elsevier Ltd. - 0360-1323 .- 1873-684X. ; 149, s. 79-89
  • Journal article (peer-reviewed)abstract
    • This paper presents a systematic experimental study on stream-wise flow development and spatial structure of triple buoyant plumes. Two-dimensional Particle Image Velocimetry (2-D PIV) is employed to resolve velocity fields. Stream-wise axial velocity profiles, flow structure, flow region parameters, and self-similarity properties are analyzed at different configurations that are characterized by spacing ratios S/W (source spacing S divided by source width W). From velocity fields and axial velocity profiles, a similar stream-wise developing trend is identified in different source configurations. When near-field plumes travel downstream, axial velocities increase rapidly, off-center velocity peaks get merged with the central peak, and the number of velocity peaks reduces with the downstream distance. A compact source layout, comparing with the wide one, could enhance the near-field plumes interaction and promote the plumes deflection significantly. Fundamentally, the stream-wise spatial structure of the triple plumes initially consists of a converging region, followed by a merging region, and finally a combined region. By examining the averaged velocity fields, flow recirculation with negative axial velocities is found to scarcely exist in the converging region. Merging level Zm and quasi-combined level Zqc are analyzed quantitatively and statistically. Within the studied S/W range, the normalized Zm shows a linear increase with S/W in the formula of Zm/H=2.007(S/W)+1.173 and the normalized Zqc gives a power law increase with S/W in the formula of Zqc/H=6.035(S/W)0.4959. In addition, triple plumes are found to establish self-similarity approximately at Z = 3H with S/W of 0.2 and at Z = 4.5H with S/W of 0.5.
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38.
  • Yin, Shi, et al. (author)
  • Experimental study of thermal plumes generated by a cluster of high-rise compact buildings under moderate background wind conditions
  • 2020
  • In: Building and Environment. - : Elsevier. - 0360-1323 .- 1873-684X. ; 181
  • Journal article (peer-reviewed)abstract
    • When background wind is moderate, urban environment may face great challenges as removal ability of airborne pollutants, anthropogenic heat and moisture reduces. Under this condition, buoyancy-driven flow can break the dominant role of the background wind and affect the urban environment greatly. Knowledge of buoyant flow dynamics is essential for understanding urban wind/thermal environment and related pollutant transport. As one important buoyant flow, thermal plumes generated by a cluster of high-rise compact buildings are modelled using a laboratory water channel in this study. Flow similarity, when the buoyancy is prominent, is well satisfied. Mean flows, turbulent statistics, and rising features under different Froude (Fr) numbers are systematically explored by two-dimensional particle image velocimetry (PIV) technique. Overall, the transition of buoyancy-dominated and wind-dominated flow dynamics is observed when Fr number varies. For horizontal and vertical velocity components, peak magnitude and position are quantitatively determined on four building poles. Fr number obviously changes the relative magnitude of v-peak among four poles. Turbulence statistics, including velocity variance, turbulence kinetic energy, power spectral density, turbulent momentum flux, and turbulence production, are systematically analyzed, which show consistent results. As Fr number decreases, the plume mixing is enhanced. When particularly aiming at the plume region, smaller Fr numbers usually make the maximum velocities occur more in the plume central part rather than the boundaries. A quantitative rational function is given to describe the relation between Fr number and the rising angle, as a = 8.76/(Frq + 0.097).
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39.
  • Yin, Shi, et al. (author)
  • PIV based POD analysis of coherent structures in flow patterns generated by triple interacting buoyant plumes
  • 2019
  • In: Building and Environment. - : Elsevier BV. - 0360-1323 .- 1873-684X. ; 158, s. 165-181
  • Journal article (peer-reviewed)abstract
    • The paper performs an investigation on multi-scale coherent structures that exist in different flow patterns of triple interacting plumes. Time-averaged and turbulent flow characteristics are first obtained by the two-dimensional (2-D) particle image velocimetry (PIV) technique. Proper orthogonal decomposition (POD) method is then applied to extract the dominant coherent structures. Energy contributions, POD modes, and POD coefficients are systematically explored to understand the spatial-temporal characteristics of the coherent structures. The first three POD modes are most important in the turbulent kinetic energy (TKE). Mode 1, as the most dominant mode, can contain about 10% TKE in an average sense. In addition, the first 12–14 POD modes can totally contribute 50% TKE. Hierarchical four-scale flow structures, independent of the flow patterns, are clearly identified when mode number increases, which are successively characterized by main flows (the 1st scale), larger-scale outer-side vortex rings (the 2nd scale), shear-layer vortex shedding (the 3rd scale), and smaller-scale homogeneous turbulent vortices (the 4th scale). Frequency spectra analysis confirms the co-existence of lower-frequency and higher-frequency periodic cycles in one certain POD mode. Dominant frequencies of the first three POD coefficient series (a1–a3) fall into the range of 0.025–0.187 Hz. Probability density analysis has identified negative/positive peak probability densities of the series a1. Cumulative probabilities of negative and positive coefficients are approximately equivalent during the sampling period. 
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40.
  • Yin, Shi, et al. (author)
  • Unsteady large-scale flow patterns and dynamic vortex movement in near-field triple buoyant plumes
  • 2018
  • In: Building and Environment. - : Elsevier Ltd. - 0360-1323 .- 1873-684X. ; 142, s. 288-300
  • Journal article (peer-reviewed)abstract
    • Unsteady flow patterns of interacting buoyant plumes are important for buoyant ventilation and particularly influence pollutant and heat transports in indoor and outdoor environments. This study reveals fundamental large-scale flow patterns in triple building plumes, investigates vortex moving trends during the pattern transition processes, and explores possible mechanisms of pattern diversity by two-dimensional (2-D) Particle Image Velocimetry (PIV). Total five tests are studied, including three different heat strengths Q (180, 90, and 30 W) and three source layouts characterized by the ratios of source spacing S to source width W (0.2, 0.5, and 1.0). Streamline distributions and axial velocity profiles clearly reveal three fundamental global flow patterns: a right-slanting asymmetrical flow pattern, a left-slanting asymmetrical flow pattern, and an axisymmetric flow pattern. Correspondingly, it indicates four basic transition processes, i.e., right-to-center, left-to-center, center-to-right, and center-to-left transitions (“center” represents the axisymmetric pattern). A novel vortex tracking method, based on lambda-2 (λ2) criterion and principles of the PIV technique, is developed and successfully applied to qualitatively track the vortex moving trends during the transition processes. The regular vortex moving trends are found to be reasonably consistent with the global pattern transition trends. The flow pattern diversity is speculated to be mainly driven by unstable heat source wall flows and downstream swaying motions in this study. These critical unstable motions are considered to probably relate to unstable lateral entrainment and vortex interaction, particularly beside the central plume. Consistently, the regular vortex moving trends are usually observed in and around the central plume.
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41.
  • You, Fang, et al. (author)
  • Icon Design Recommendations for Central Consoles of Intelligent Vehicles
  • 2020
  • In: Human Interaction, Emerging Technologies and Future Applications II. - Cham : Springer. - 9783030442668 - 9783030442675 ; , s. 285-291
  • Conference paper (peer-reviewed)abstract
    • Advances in vehicle technology open up both opportunities and challenges for human-machine interface (HMI) design in intelligent vehicles. Design guidelines for icons in central consoles of vehicles have been discussed in human-computer interaction community, but more study and innovation are needed in icon design. In this paper, we investigated the displayed line thickness and size of icons in the intelligent vehicle’s central control screen, based on international design guidelines and standards. The experiment involved 17 participants performing simulated in-vehicle secondary task. The result from the experiments shows that the usability of icons increases and the driver’s workload decreases as the icons get larger. We also found a set of recommended values for size and line thickness of icons in this context. Future work will focus on providing design guidelines for other aspects of HMI design in intelligent vehicles.
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42.
  • Zhang, Litianyi, et al. (author)
  • Enabling Real-Time Quality-of-Service and Fine-Grained Aggregation for Wireless TSN
  • 2022
  • In: Sensors. - : MDPI AG. - 1424-8220. ; 22:10
  • Journal article (peer-reviewed)abstract
    • Wireless Time-Sensitive Networking (WTSN) has emerged as a promising technology for Industrial Internet of Things (IIoT) applications. To meet the latency requirements of WTSN, wireless local area network (WLAN) such as IEEE 802.11 protocol with the time division multiple access (TDMA) mechanism is shown to be a practical solution. In this paper, we propose the RT-WiFiQA protocol with two novel schemes to improve the latency and reliability performance: real-time quality of service (RT-QoS) and fine-grained aggregation (FGA) for TDMA-based 802.11 systems. The RT-QoS is designed to guarantee the quality-of-service requirements of different traffic and to support the FGA mechanism. The FGA mechanism aggregates frames for different stations to reduce the physical layer transmission overhead. The trade-off between the reliability and FGA packet size is analyzed with numerical results. Specifically, we derive a critical threshold such that the FGA can achieve higher reliability when the aggregated packet size is smaller than the critical threshold. Otherwise, the non-aggregation scheme outperforms the FGA scheme. Extensive experiments are conducted on the commercial off-the-shelf 802.11 interface. The experiment results show that compared with the existing TDMA-based 802.11 system, the developed RT-WiFiQA protocol can achieve deterministic bounded real-time latency and greatly improves the reliability performance.
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