| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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| 3. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 4. |
- Ablikim, M., et al.
(författare)
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Measurements of (XcJ)-> K+K-K+K- decays
- 2006
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Ingår i: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 642:3, s. 197-202
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Tidskriftsartikel (refereegranskat)abstract
- Using 14M psi(2S) events taken with the BESII detector, chi(cJ) -> 2(K+K-) decays are studied. For the four-kaon final state, the branching fractions are B(chi(c0,1,2) ->.2(K+K-)) = (3.48 +/- 0.23 +/- 0.47) x 10(-3), (0.70 +/- 0.13 +/- 0.10) x 10(-3), and (2.17 +/- 0.20 +/- 0.31) x 10(-3). For the phi K+K- final state, the branching fractions, which are measured for the first time, are B(chi(c0,1,2) -> phi K+K-) = (1.03 +/- 0.22 +/- 0.15) x 10(-3), (0.46 +/- 0.16 +/- 0.06) x 10(-3), and (1.67 +/- 0.26 +/- 0.24) x 10(-4). For the phi phi final state, B(chi(c0,2) -> phi phi) = (0.94 +/- 0.21 +/- 0.13) x 10(-3) and (1.70 +/- 0.30 +/- 0.25) x 10(-3).
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| 5. |
- Kristan, Matej, et al.
(författare)
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The Visual Object Tracking VOT2015 challenge results
- 2015
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Ingår i: Proceedings 2015 IEEE International Conference on Computer Vision Workshops ICCVW 2015. - : IEEE. - 9780769557205 ; , s. 564-586
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge 2015, VOT2015, aims at comparing short-term single-object visual trackers that do not apply pre-learned models of object appearance. Results of 62 trackers are presented. The number of tested trackers makes VOT 2015 the largest benchmark on short-term tracking to date. For each participating tracker, a short description is provided in the appendix. Features of the VOT2015 challenge that go beyond its VOT2014 predecessor are: (i) a new VOT2015 dataset twice as large as in VOT2014 with full annotation of targets by rotated bounding boxes and per-frame attribute, (ii) extensions of the VOT2014 evaluation methodology by introduction of a new performance measure. The dataset, the evaluation kit as well as the results are publicly available at the challenge website(1).
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| 6. |
- Ching, Tao-Chung, et al.
(författare)
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The JCMT BISTRO-2 Survey: Magnetic Fields of the Massive DR21 Filament
- 2022
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Ingår i: Astrophysical Journal. - : American Astronomical Society. - 1538-4357 .- 0004-637X. ; 941:2
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Tidskriftsartikel (refereegranskat)abstract
- We present 850 mu m dust polarization observations of the massive DR21 filament from the B-fields In STar-forming Region Observations (BISTRO) survey, using the POL-2 polarimeter and the SCUBA-2 camera on the James Clerk Maxwell Telescope. We detect ordered magnetic fields perpendicular to the parsec-scale ridge of the DR21 main filament. In the subfilaments, the magnetic fields are mainly parallel to the filamentary structures and smoothly connect to the magnetic fields of the main filament. We compare the POL-2 and Planck dust polarization observations to study the magnetic field structures of the DR21 filament on 0.1-10 pc scales. The magnetic fields revealed in the Planck data are well-aligned with those of the POL-2 data, indicating a smooth variation of magnetic fields from large to small scales. The plane-of-sky magnetic field strengths derived from angular dispersion functions of dust polarization are 0.6-1.0 mG in the DR21 filament and similar to 0.1 mG in the surrounding ambient gas. The mass-to-flux ratios are found to be magnetically supercritical in the filament and slightly subcritical to nearly critical in the ambient gas. The alignment between column density structures and magnetic fields changes from random alignment in the low-density ambient gas probed by Planck to mostly perpendicular in the high-density main filament probed by James Clerk Maxwell Telescope. The magnetic field structures of the DR21 filament are in agreement with MHD simulations of a strongly magnetized medium, suggesting that magnetic fields play an important role in shaping the DR21 main filament and subfilaments.
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| 7. |
- Kanoni, Stavroula, et al.
(författare)
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Implicating genes, pleiotropy, and sexual dimorphism at blood lipid loci through multi-ancestry meta-analysis.
- 2022
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Ingår i: Genome biology. - : Springer Science and Business Media LLC. - 1474-760X .- 1465-6906 .- 1474-7596. ; 23:1
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Tidskriftsartikel (refereegranskat)abstract
- Genetic variants within nearly 1000 loci are known to contribute to modulation of blood lipid levels. However, the biological pathways underlying these associations are frequently unknown, limiting understanding of these findings and hindering downstream translational efforts such as drug target discovery.To expand our understanding of the underlying biological pathways and mechanisms controlling blood lipid levels, we leverage a large multi-ancestry meta-analysis (N=1,654,960) of blood lipids to prioritize putative causal genes for 2286 lipid associations using six gene prediction approaches. Using phenome-wide association (PheWAS) scans, we identify relationships of genetically predicted lipid levels to other diseases and conditions. We confirm known pleiotropic associations with cardiovascular phenotypes and determine novel associations, notably with cholelithiasis risk. We perform sex-stratified GWAS meta-analysis of lipid levels and show that 3-5% of autosomal lipid-associated loci demonstrate sex-biased effects. Finally, we report 21 novel lipid loci identified on the X chromosome. Many of the sex-biased autosomal and X chromosome lipid loci show pleiotropic associations with sex hormones, emphasizing the role of hormone regulation in lipid metabolism.Taken together, our findings provide insights into the biological mechanisms through which associated variants lead to altered lipid levels and potentially cardiovascular disease risk.
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| 8. |
- Karoly, Janik, et al.
(författare)
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The JCMT BISTRO Survey: Studying the Complex Magnetic Field of L43
- 2023
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Ingår i: Astrophysical Journal. - 1538-4357 .- 0004-637X. ; 952:1
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Tidskriftsartikel (refereegranskat)abstract
- We present observations of polarized dust emission at 850 mu m from the L43 molecular cloud, which sits in the Ophiuchus cloud complex. The data were taken using SCUBA-2/POL-2 on the James Clerk Maxwell Telescope as a part of the BISTRO large program. L43 is a dense (N-H2 similar to 10(22) - 10(23) cm(-2)) complex molecular cloud with a submillimeter-bright starless core and two protostellar sources. There appears to be an evolutionary gradient along the isolated filament that L43 is embedded within, with the most evolved source closest to the Sco OB2 association. One of the protostars drives a CO outflow that has created a cavity to the southeast. We see a magnetic field that appears to be aligned with the cavity walls of the outflow, suggesting interaction with the outflow. We also find a magnetic field strength of up to similar to 160 +/- 30 mu G in the main starless core and up to similar to 90 +/- 40 mu G in the more diffuse, extended region. These field strengths give magnetically super- and subcritical values, respectively, and both are found to be roughly trans-Alfvenic. We also present a new method of data reduction for these denser but fainter objects like starless cores.
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| 9. |
- Pecunia, Vincenzo, et al.
(författare)
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Roadmap on energy harvesting materials
- 2023
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Ingår i: Journal of Physics. - : IOP Publishing. - 2515-7639. ; 6:4
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Tidskriftsartikel (refereegranskat)abstract
- Ambient energy harvesting has great potential to contribute to sustainable development and address growing environmental challenges. Converting waste energy from energy-intensive processes and systems (e.g. combustion engines and furnaces) is crucial to reducing their environmental impact and achieving net-zero emissions. Compact energy harvesters will also be key to powering the exponentially growing smart devices ecosystem that is part of the Internet of Things, thus enabling futuristic applications that can improve our quality of life (e.g. smart homes, smart cities, smart manufacturing, and smart healthcare). To achieve these goals, innovative materials are needed to efficiently convert ambient energy into electricity through various physical mechanisms, such as the photovoltaic effect, thermoelectricity, piezoelectricity, triboelectricity, and radiofrequency wireless power transfer. By bringing together the perspectives of experts in various types of energy harvesting materials, this Roadmap provides extensive insights into recent advances and present challenges in the field. Additionally, the Roadmap analyses the key performance metrics of these technologies in relation to their ultimate energy conversion limits. Building on these insights, the Roadmap outlines promising directions for future research to fully harness the potential of energy harvesting materials for green energy anytime, anywhere.
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| 10. |
- Peng, Shi-Yu, et al.
(författare)
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Reduced motor cortex GABABR function following chronic alcohol exposure
- 2021
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Ingår i: Molecular Psychiatry. - : SPRINGERNATURE. - 1359-4184 .- 1476-5578. ; 26:2, s. 383-395
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Tidskriftsartikel (refereegranskat)abstract
- The GABA(B) receptor (GABA(B)R) agonist baclofen has been used to treat alcohol and several other substance use disorders (AUD/SUD), yet its underlying neural mechanism remains unclear. The present study aimed to investigate cortical GABA(B)R dynamics following chronic alcohol exposure. Ex vivo brain slice recordings from mice chronically exposed to alcohol revealed a reduction in GABA(B)R-mediated currents, as well as a decrease of GABA(B1/2)R and G-protein-coupled inwardly rectifying potassium channel 2 (GIRK2) activities in the motor cortex. Moreover, our data indicated that these alterations could be attributed to dephosphorylation at the site of serine 783 (ser-783) in GABA(B2) subunit, which regulates the surface expression of GABA(B)R. Furthermore, a human study using paired-pulse-transcranial magnetic stimulation (TMS) analysis further demonstrated a reduced cortical inhibition mediated by GABA(B)R in patients with AUD. Our findings provide the first evidence that chronic alcohol exposure is associated with significantly impaired cortical GABA(B)R function. The ability to promote GABA(B)R signaling may account for the therapeutic efficacy of baclofen in AUD.
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| 11. |
- You, Xiaohu, et al.
(författare)
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Towards 6G wireless communication networks: vision, enabling technologies, and new paradigm shifts
- 2021
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Ingår i: Science China Information Sciences. - : Science Press. - 1674-733X .- 1869-1919. ; 64:1
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Forskningsöversikt (refereegranskat)abstract
- The fifth generation (5G) wireless communication networks are being deployed worldwide from 2020 and more capabilities are in the process of being standardized, such as mass connectivity, ultra-reliability, and guaranteed low latency. However, 5G will not meet all requirements of the future in 2030 and beyond, and sixth generation (6G) wireless communication networks are expected to provide global coverage, enhanced spectral/energy/cost efficiency, better intelligence level and security, etc. To meet these requirements, 6G networks will rely on new enabling technologies, i.e., air interface and transmission technologies and novel network architecture, such as waveform design, multiple access, channel coding schemes, multi-antenna technologies, network slicing, cell-free architecture, and cloud/fog/edge computing. Our vision on 6G is that it will have four new paradigm shifts. First, to satisfy the requirement of global coverage, 6G will not be limited to terrestrial communication networks, which will need to be complemented with non-terrestrial networks such as satellite and unmanned aerial vehicle (UAV) communication networks, thus achieving a space-air-ground-sea integrated communication network. Second, all spectra will be fully explored to further increase data rates and connection density, including the sub-6 GHz, millimeter wave (mmWave), terahertz (THz), and optical frequency bands. Third, facing the big datasets generated by the use of extremely heterogeneous networks, diverse communication scenarios, large numbers of antennas, wide bandwidths, and new service requirements, 6G networks will enable a new range of smart applications with the aid of artificial intelligence (AI) and big data technologies. Fourth, network security will have to be strengthened when developing 6G networks. This article provides a comprehensive survey of recent advances and future trends in these four aspects. Clearly, 6G with additional technical requirements beyond those of 5G will enable faster and further communications to the extent that the boundary between physical and cyber worlds disappears.
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| 12. |
- Chen, Yufeng, et al.
(författare)
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Oral fungal profiling and risk of nasopharyngeal carcinoma : a population-based case-control study
- 2023
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Ingår i: EBioMedicine. - 2352-3964. ; 96
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Dysbiosis of the oral mycobiome has been linked to some diseases, including cancers. However, the role of oral fungal communities in nasopharyngeal carcinoma (NPC) carcinogenesis has not previously been investigated.METHODS: We characterized the oral salivary fungal mycobiome in 476 untreated incident NPC patients and 537 population-based controls using fungal internal transcribed spacer (ITS)-2 sequencing. The relationship between oral fungal mycobiome and the risk of NPC was assessed through bioinformatic and biostatistical analyses.FINDINGS: We found that lower fungal alpha diversity was associated with an increased odds of NPC [lower vs. higher: observed features (adjusted odds ratio [OR] = 5.81, 95% confidence interval [CI] = 3.60-9.38); Simpson diversity (1.53, 1.03-2.29); Shannon diversity (2.03, 1.35-3.04)]. We also observed a significant difference in global fungal community patterns between cases and controls based on Bray-Curtis dissimilarity (P < 0.001). Carriage of oral fungal species, specifically, Saccharomyces cerevisiae, Candida tropicalis, Lodderomyces elongisporus, Candida albicans, and Fusarium poae, was associated with significantly higher odds of NPC, with ORs ranging from 1.56 to 4.66. Individuals with both low fungal and low bacterial alpha diversity had a profoundly elevated risk of NPC.INTERPRETATION: Our results suggest that dysbiosis in the oral mycobiome, characterized by a loss of fungal community diversity and overgrowth of several fungal organisms, is associated with a substantially increased risk of NPC.FUNDING: This work was funded by the US National Institutes of Health, the Swedish Research Council, the High-level Talents Research Start-up Project of Fujian Medical University, and the China Scholarship Council.
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| 13. |
- de Vries, Paul S., et al.
(författare)
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Multiancestry Genome-Wide Association Study of Lipid Levels Incorporating Gene-Alcohol Interactions
- 2019
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Ingår i: American Journal of Epidemiology. - : Oxford University Press. - 0002-9262 .- 1476-6256. ; 188:6, s. 1033-1054
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Tidskriftsartikel (refereegranskat)abstract
- A person's lipid profile is influenced by genetic variants and alcohol consumption, but the contribution of interactions between these exposures has not been studied. We therefore incorporated gene-alcohol interactions into a multiancestry genome-wide association study of levels of high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and triglycerides. We included 45 studies in stage 1 (genome-wide discovery) and 66 studies in stage 2 (focused follow-up), for a total of 394,584 individuals from 5 ancestry groups. Analyses covered the period July 2014-November 2017. Genetic main effects and interaction effects were jointly assessed by means of a 2-degrees-of-freedom (df) test, and a 1-df test was used to assess the interaction effects alone. Variants at 495 loci were at least suggestively associated (P < 1 x 10(-6)) with lipid levels in stage 1 and were evaluated in stage 2, followed by combined analyses of stage 1 and stage 2. In the combined analysis of stages 1 and 2, a total of 147 independent loci were associated with lipid levels at P < 5 x 10(-8) using 2-df tests, of which 18 were novel. No genome-wide-significant associations were found testing the interaction effect alone. The novel loci included several genes (proprotein convertase subtilisin/kexin type 5 (PCSK5), vascular endothelial growth factor B (VEGFB), and apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (APOBEC1) complementation factor (A1CF)) that have a putative role in lipid metabolism on the basis of existing evidence from cellular and experimental models.
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| 14. |
- Ding, Jiangwei, et al.
(författare)
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All Roads Lead to Rome? : Genes Causing Dravet Syndrome and Dravet Syndrome-Like Phenotypes
- 2022
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Ingår i: Frontiers in Neurology. - : Frontiers Media S.A.. - 1664-2295. ; 13
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Forskningsöversikt (refereegranskat)abstract
- Background: Dravet syndrome (DS) is a severe epileptic encephalopathy mainly caused by haploinsufficiency of the gene SCN1A, which encodes the voltage-gated sodium channel NaV1. 1 in the brain. While SCN1A mutations are known to be the primary cause of DS, other genes that may cause DS are poorly understood. Several genes with pathogenic mutations result in DS or DS-like phenotypes, which may require different drug treatment approaches. Therefore, it is urgent for clinicians, especially epilepsy specialists to fully understand these genes involved in DS in addition to SCN1A. Particularly for healthcare providers, a deep understanding of these pathogenic genes is useful in properly selecting and adjusting drugs in a more effective and timely manner.Objective: The purpose of this study was to identify genes other than SCN1A that may also cause DS or DS-like phenotypes. Methods: A comprehensive search of relevant Dravet syndrome and severe myoclonic epilepsy in infancy was performed in PubMed, until December 1, 2021. Two independent authors performed the screening for potentially eligible studies. Disagreements were decided by a third, more professional researcher or by all three. The results reported by each study were narratively summarized.Results: A PubMed search yielded 5,064 items, and other sources search 12 records. A total of 29 studies published between 2009 and 2021 met the inclusion criteria. Regarding the included articles, seven studies on PCDH19, three on SCN2A, two on SCN8A, five on SCN1B, two on GABRA1, three on GABRB3, three on GABRG2, and three on STXBP1 were included. Only one study was recorded for CHD2, CPLX1, HCN1 and KCNA2, respectively. It is worth noting that a few articles reported on more than one epilepsy gene.Conclusion: DS is not only identified in variants of SCN1A, but other genes such as PCDH19, SCN2A, SCN8A, SCN1B, GABRA1, GABRB3, GABRG2, KCNA2, CHD2, CPLX1, HCN1A, STXBP1 can also be involved in DS or DS-like phenotypes. As genetic testing becomes more widely available, more genes associated with DS and DS-like phenotypes may be identified and gene-based diagnosis of subtypes of phenotypes in this spectrum may improve the management of these diseases in the future.
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| 15. |
- Hering, Alessa, et al.
(författare)
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Learn2Reg: comprehensive multi-task medical image registration challenge, dataset and evaluation in the era of deep learning
- 2023
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Ingår i: IEEE Transactions on Medical Imaging. - : Institute of Electrical and Electronics Engineers (IEEE). - 0278-0062 .- 1558-254X. ; 42:3, s. 697-712
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Tidskriftsartikel (refereegranskat)abstract
- Image registration is a fundamental medical image analysis task, and a wide variety of approaches have been proposed. However, only a few studies have comprehensively compared medical image registration approaches on a wide range of clinically relevant tasks. This limits the development of registration methods, the adoption of research advances into practice, and a fair benchmark across competing approaches. The Learn2Reg challenge addresses these limitations by providing a multi-task medical image registration data set for comprehensive characterisation of deformable registration algorithms. A continuous evaluation will be possible at https:// learn2reg.grand-challenge.org. Learn2Reg covers a wide range of anatomies (brain, abdomen, and thorax), modalities (ultrasound, CT, MR), availability of annotations, as well as intra- and inter-patient registration evaluation. We established an easily accessible framework for training and validation of 3D registration methods, which enabled the compilation of results of over 65 individual method submissions from more than 20 unique teams. We used a complementary set of metrics, including robustness, accuracy, plausibility, and runtime, enabling unique insight into the current state-of-the-art of medical image registration. This paper describes datasets, tasks, evaluation methods and results of the challenge, as well as results of further analysis of transferability to new datasets, the importance of label supervision, and resulting bias. While no single approach worked best across all tasks, many methodological aspects could be identified that push the performance of medical image registration to new state-of-the-art performance. Furthermore, we demystified the common belief that conventional registration methods have to be much slower than deep-learning-based methods.
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| 16. |
- Hu, Li-Xin, et al.
(författare)
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What is in Nigerian waters? Target and non-target screening analysis for organic chemicals
- 2021
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Ingår i: Chemosphere. - : Elsevier BV. - 0045-6535 .- 1879-1298. ; 284
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Tidskriftsartikel (refereegranskat)abstract
- Emerging organic contaminants (e.g., active pharmaceutical ingredients and personal care products ingredients) are ubiquitous in the environment and potentially harmful to ecosystems, have gained increasing public attention worldwide. Nevertheless, there is a scarcity of data on these contaminants in Africa. In this study, various types of water samples (wastewater, surface water and tap water) collected from Lagos, Nigeria were analyzed for these chemicals by both target and non-target analysis on an UHPLC-Orbitrap-MS/MS. In total, 109 compounds were identified by non-target screening using the online database mzCloud. Level 1 identification confidence was achieved for 13 compounds for which reference standards were available and level 2 was achieved for the rest. In the quantitative analysis, 18 of 38 target compounds were detected, including the parent compounds and their metabolites. Acetaminophen, sulfamethoxazole, acesulfame, and caffeine were detected in all samples with their highest concentrations at 8000, 5300, 16, and 7700 μg/L in wastewater, 140000, 3300, 7.7, and 12000 μg/L in surface water, and 66, 62, 0.17 and 1000 μg/L in tap water, respectively. The occurrence of psychoactive substances, anticancer treatments, antiretrovirals, antihypertensives, antidiabetics and their metabolites were reported in Nigeria for the first time. These results indicate poor wastewater treatment and management in Nigeria, and provide a preliminary profile of organic contaminants occurring in Nigerian waters. The findings from this study urge more future research on chemical pollution in the aquatic environments in Nigeria.
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| 17. |
- Huang, Yong-Qing, et al.
(författare)
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The Evaluation of Basic Fibroblast Growth Factor and Fibroblastic Growth Factor Receptor 1 Levels in Saliva and Serum of Patients with Salivary Gland Tumor
- 2012
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Ingår i: DNA and Cell Biology. - : Mary Ann Liebert Inc. - 1044-5498 .- 1557-7430. ; 31:4, s. 520-523
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Tidskriftsartikel (refereegranskat)abstract
- Basic fibroblast growth factor (FGF2) is a well-known endothelial mitogen that regulates endothelial cell proliferation, migration, differentiation, and survival. In the present study, we investigated the levels of FGF2 and fibroblastic growth factor receptor 1 (FGFR1) in saliva and serum of patients with salivary gland tumors. Saliva and serum samples were collected from 43 patients with salivary gland tumors and 40 healthy volunteers. The FGF2 and FGFR1 concentrations in saliva and serum samples were measured by enzyme-linked immunosorbent assay. We found that the levels of FGF2 and FGFR1 in saliva and serum from patients with salivary gland tumors were significantly higher than those from healthy control subjects. These results suggest that salivary FGF2 and FGFR1 can be used as potential biomarkers in the diagnosis of salivary gland tumors.
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| 18. |
- Liang, Xifeng, et al.
(författare)
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On the aerodynamic loads when a high speed train passes under an overhead bridge
- 2020
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Ingår i: Journal of Wind Engineering and Industrial Aerodynamics. - : Elsevier BV. - 0167-6105. ; 202
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Tidskriftsartikel (refereegranskat)abstract
- The aerodynamic loads on the overhead bridge bottom surface induced by train passage are reported in this paper. Both moving model test and numerical simulation approaches at the 1:20 scale are used. The numerical work is validated through both mesh independence tests and comparison with experimental data. Typical pressure variation curves are plotted and compared with previous studies. The peak pressure values’ dependence on the Reynolds number is considered through four sets of experiments with different train running speeds. The peak pressure coefficient distribution law for the bridge bottom surface is presented. Differences in the pressure distribution in different bridge bottom areas are explained based on more detailed flow field information. The influence of the bridge height on the aerodynamic load magnitude and time interval is presented. Moreover, the application of the CEN Standard to practical engineering issues is discussed.
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| 19. |
- Liu, Jian-li, et al.
(författare)
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China's most typical nonferrous organic-metal facilities own specific microbial communities
- 2018
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Ingår i: Scientific Reports. - : Nature Publishing Group. - 2045-2322. ; 8:1
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Tidskriftsartikel (refereegranskat)abstract
- The diversity and function of microorganisms have yet to be explored at non-ferrous metal mining facilities (NMMFs), which are the world's largest and potentially most toxic sources of co-existing metal(loid)s and flotation reagents (FRs). The diversity and inferred functions of different bacterial communities inhabiting two types of sites (active and abandoned) in Guangxi province (China) were investigated for the first time. Here we show that the structure and diversity of bacteria correlated with the types of mine sites, metal(loid)s, and FRs concentrations; and best correlated with the combination of pH, Cu, Pb, and Mn. Combined microbial coenobium may play a pivotal role in NMMFs microbial life. Arenimonas, specific in active mine sites and an acidophilic bacterium, carries functions able to cope with the extreme conditions, whereas Latescibacteria specific in abandoned sites can degrade organics. Such a bacterial consortium provides new insights to develop cost-effective remediation strategies of co-contaminated sites that currently remain intractable for bioremediation.
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| 20. |
- Qian, Junpeng, et al.
(författare)
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Vision-Based Contactless Pose Estimation for Human Thermal Discomfort
- 2020
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Ingår i: Atmosphere. - : MDPI. - 2073-4433. ; 11:4
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Tidskriftsartikel (refereegranskat)abstract
- Real-time and effective human thermal discomfort detection plays a critical role in achieving energy efficient control of human centered intelligent buildings because estimation results can provide effective feedback signals to heating, ventilation and air conditioning (HVAC) systems. How to detect occupant thermal discomfort is a challenge. Unfortunately, contact or semi-contact perception methods are inconvenient in practical application. From the contactless perspective, a kind of vision-based contactless human discomfort pose estimation method was proposed in this paper. Firstly, human pose data were captured from a vision-based sensor, and corresponding human skeleton information was extracted. Five thermal discomfort-related human poses were analyzed, and corresponding algorithms were constructed. To verify the effectiveness of the algorithms, 16 subjects were invited for physiological experiments. The validation results show that the proposed algorithms can recognize the five human poses of thermal discomfort.
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| 21. |
- Su, Linjia, et al.
(författare)
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GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer
- 2022
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Ingår i: Journal of Nanobiotechnology. - : Springer Nature. - 1477-3155. ; 20:1
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Tidskriftsartikel (refereegranskat)abstract
- Practice of tumor-targeted suicide gene therapy is hampered by unsafe and low efficient delivery of plasmid DNA (pDNA). Using HIV-Tat-derived peptide (Tat) to non-covalently form Tat/pDNA complexes advances the delivery performance. However, this innovative approach is still limited by intracellular delivery efficiency and cell-cycle status. In this study, Tat/pDNA complexes were further condensed into smaller, nontoxic nanoparticles by Ca2+ addition. Formulated Tat/pDNA-Ca2+ nanoparticles mainly use macropinocytosis for intercellular delivery, and their macropinocytic uptake was persisted in mitosis (M-) phase and highly activated in DNA synthesis (S-) phase of cell-cycle. Over-expression or phosphorylation of a mitochondrial chaperone, 75-kDa glucose-regulated protein (GRP75), promoted monopolar spindle kinase 1 (MPS1)-controlled centrosome duplication and cell-cycle progress, but also driven cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles. Further in vivo molecular imaging based on DF (Fluc-eGFP)-TF (RFP-Rluc-HSV-ttk) system showed that Tat/pDNA-Ca2+ nanoparticles exhibited highly suicide gene therapy efficiency in mouse model xenografted with human ovarian cancer. Furthermore, arresting cell-cycle at S-phase markedly enhanced delivery performance of Tat/pDNA-Ca2+ nanoparticles, whereas targeting GRP75 reduced their macropinocytic delivery. More importantly, in vivo targeting GRP75 combined with cell-cycle or macropinocytosis inhibitors exhibited distinct suicide gene therapy efficiency. In summary, our data highlight that mitochondrial chaperone GRP75 moonlights as a biphasic driver underlying cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles in ovarian cancer.
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| 22. |
- Wang, Longxin, et al.
(författare)
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Telomere-to-telomere and haplotype-resolved genome assembly of the Chinese cork oak (Quercus variabilis)
- 2023
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Ingår i: Frontiers in Plant Science. - : Frontiers Media S.A.. - 1664-462X. ; 14
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Tidskriftsartikel (refereegranskat)abstract
- The Quercus variabilis, a deciduous broadleaved tree species, holds significant ecological and economical value. While a chromosome-level genome for this species has been made available, it remains riddled with unanchored sequences and gaps. In this study, we present a nearly complete comprehensive telomere-to-telomere (T2T) and haplotype-resolved reference genome for Q. variabilis. This was achieved through the integration of ONT ultra-long reads, PacBio HiFi long reads, and Hi-C data. The resultant two haplotype genomes measure 789 Mb and 768 Mb in length, with a contig N50 of 65 Mb and 56 Mb, and were anchored to 12 allelic chromosomes. Within this T2T haplotype-resolved assembly, we predicted 36,830 and 36,370 protein-coding genes, with 95.9% and 96.0% functional annotation for each haplotype genome. The availability of the T2T and haplotype-resolved reference genome lays a solid foundation, not only for illustrating genome structure and functional genomics studies but also to inform and facilitate genetic breeding and improvement of cultivated Quercus species.
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| 23. |
- Xie, Zhe, et al.
(författare)
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Combined effect of salinity and hypoxia on digestive enzymes and intestinal microbiota in the oyster Crassostrea hongkongensis
- 2023
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Ingår i: Environmental Pollution. - 0269-7491 .- 1873-6424. ; 331
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Tidskriftsartikel (refereegranskat)abstract
- Anthropologic activities caused frequent eutrophication in coastal and estuarine waters, resulting in diel-cycling hypoxia. Given global climate change, extreme weather events often occur, thus salinity fluctuation frequently breaks out in these waters. This study aimed to evaluate the combined effects of salinity and hypoxia on intestinal microbiota and digestive enzymes of Crassostrea hongkongensis. Specifically, we sequenced 16 S rRNA of intestinal microbiota and measured the digestive enzymes trypsin (TRS), lipase (LPS) and amylase (AMY) in oysters exposed for 28 days to three salinities (10, 25 and 35) and two dissolved oxygen conditions, normoxia (6 mg/L) and hypoxia (6 mg/L for 12 h, 2 mg/L for 12 h). Oysters in normoxia and salinity of 25 were treated as control. After 28-day exposure, for microbial components, Fusobacteriota, Firmicutes, Bacteroidota, Proteobacteria and Actinobacteriota comprised the majority for all experimental groups. Compared with the control group, the diversity and structure of intestinal microbiota tended to change in all treated groups. The species richness in C. hongkongensis intestine also changed. It was the most significant that high salinity increased Proteobacteria proportion while low salinity and hypoxia increased Fusobacteriota but decreased Proteobacteria, respectively. Additionally, Actinobacteriota was sensitive and changed under environmental stressor (P < 0.01). The prediction results on intestinal microbiota showed that, all functions of oysters were up-regulated to distinct degrees under low/high salinity with hypoxia. According to the KEGG prediction, cellular processes were more active and energy metabolism upregulated, indicating the adaptation of C. hongkongensis to environmental change. Periodical hypoxia and low/high salinity had complex effect on the digestive enzymes, in which the activity of TRS and LPS decreased while AMY increased. High/low salinity and periodical hypoxia can change the secretion of digestive enzymes and influence intestinal microbial diversity and species richness of C. hongkongensis, deducing the chronic adverse effects on the digestive physiology in long-term exposure.
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| 24. |
- Yang, Bin, et al.
(författare)
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Enhanced effects of footwarmer by wearing sandals in winter office : a Swedish case study
- 2021
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Ingår i: Indoor + Built Environment. - : Sage Publications. - 1420-326X .- 1423-0070. ; 30:7, s. 875-885
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Tidskriftsartikel (refereegranskat)abstract
- Human-centred thermal environment conditioning can guarantee thermal comfort needs of human occupants in their micro-environments by using localized heating/cooling devices. Meanwhile, less intensified thermal conditioning of unoccupied surrounding environments can achieve heating/cooling energy efficiency. The concept was originated from task/ambient conditioning, which was developed for personal comfort systems. Most of the localized heating/cooling devices are workstation based or chair based. Task conditioning would become more closely to targets (human bodies) by using special clothing materials or thermoelectric elements. From thermal physiological viewpoints, thermal stimulus to thermally sensitive body parts may generate better results for not only local thermal comfort but also for whole body thermal comfort. Thermal stimulus to the extremities (feet) has demonstrated good thermal comfort effects. Scandinavians are accustomed to outdoor harsh environments and prefer wearing heavy shoes' outdoors in winter. They have the habit of changing heavy shoes to sandals when entering offices, which give the opportunity to enhance localized heating effects of footwarmers by reducing shoes' thermal resistance. Climatic chamber tests with 32 Nordic subjects were performed under different indoor ambient heating temperatures with/without the aid of footwarmers. With footwarmers and sandals, indoor heating temperature at 16°C was acceptable, which achieved energy efficient thermal comfort.
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| 25. |
- Yang, Bin, et al.
(författare)
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Influence of relayed fans and low level exhausts on performance of attachment ventilation under heating mode
- 2021
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Ingår i: Journal of Building Engineering. - : Elsevier. - 2352-7102. ; 36
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Tidskriftsartikel (refereegranskat)abstract
- Compared with displacement ventilation, attachment ventilation characterized by high supply air momentum has the potential to generate warm air lake on the floor under winter heating mode. However, the airflow can not keep attaching to the floor and moves upwards by thermal buoyancy. The influence of relayed fans and low level exhausts on performance of attachment ventilation under heating mode was explored. One/two relayed fans and five dimensionless exhaust heights were investigated. Performance evaluating index include vertical temperature gradient, temperature efficiency, predicted mean vote (PMV), predicted percentage of dissatisfied (PPD) and Draught risk (DR). Simulation results showed that relayed fans and low level exhausts effectively increase the attachment length of warm air lake in winter. Operation of relayed fans reduces vertical temperature difference, maintaining the head and foot temperature difference less than 3 °C. Low level exhausts effectively increase occupant zone temperature, reduce airflow short circuit and improve temperature efficiency. The operation of relayed fans slightly increase the temperature efficiency under high level exhausts. Two relayed fans decrease the temperature efficiency under low level exhausts. Better performance can be achieved by using only one relayed fan.
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| 26. |
- Zhu, Zhenshuo, et al.
(författare)
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Histone demethylase complexes KDM3A and KDM3B cooperate with OCT4/SOX2 to define a pluripotency gene regulatory network
- 2021
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Ingår i: The FASEB Journal. - : John Wiley & Sons. - 0892-6638 .- 1530-6860. ; 35:6
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Tidskriftsartikel (refereegranskat)abstract
- The pluripotency gene regulatory network of porcine induced pluripotent stem cells(piPSCs), especially in epigenetics, remains elusive. To determine the biological function of epigenetics, we cultured piPSCs in different culture conditions. We found that activation of pluripotent gene- and pluripotency-related pathways requires the erasure of H3K9 methylation modification which was further influenced by mouse embryonic fibroblast (MEF) served feeder. By dissecting the dynamic change of H3K9 methylation during loss of pluripotency, we demonstrated that the H3K9 demethylases KDM3A and KDM3B regulated global H3K9me2/me3 level and that their co-depletion led to the collapse of the pluripotency gene regulatory network. Immunoprecipitation-mass spectrometry (IP-MS) provided evidence that KDM3A and KDM3B formed a complex to perform H3K9 demethylation. The genome-wide regulation analysis revealed that OCT4 (O) and SOX2 (S), the core pluripotency transcriptional activators, maintained the pluripotent state of piPSCs depending on the H3K9 hypomethylation. Further investigation revealed that O/S cooperating with histone demethylase complex containing KDM3A and KDM3B promoted pluripotency genes expression to maintain the pluripotent state of piPSCs. Together, these data offer a unique insight into the epigenetic pluripotency network of piPSCs.
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| 27. |
- Cai, Yixiao, et al.
(författare)
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Strategy towards independent electrical stimulation from cochlear implants : Guided auditory neuron growth on topographically modified nanocrystalline diamond
- 2016
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Ingår i: Acta Biomaterialia. - : Elsevier BV. - 1742-7061 .- 1878-7568. ; 31, s. 211-220
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Tidskriftsartikel (refereegranskat)abstract
- Cochlear implants (CI) have been used for several decades to treat patients with profound hearing loss. Nevertheless, results vary between individuals, and fine hearing is generally poor due to the lack of discrete neural stimulation from the individual receptor hair cells. A major problem is the deliverance of independent stimulation signals to individual auditory neurons. Fine hearing requires significantly more stimulation contacts with intimate neuron/electrode interphases from ordered axonal re-growth, something current CI technology cannot provide.Here, we demonstrate the potential application of micro-textured nanocrystalline diamond (NCD) surfaces on CI electrode arrays. Such textured NCD surfaces consist of micrometer-sized nail-head-shaped pillars (size 5 5 lm2) made with sequences of micro/nano-fabrication processes, including sputtering, photolithography and plasma etching.The results show that human and murine inner-ear ganglion neurites and, potentially, neural progenitor cells can attach to patterned NCD surfaces without an extracellular matrix coating. Microscopic methods revealed adhesion and neural growth, specifically along the nail-head-shaped NCD pillars in an ordered manner, rather than in non-textured areas. This pattern was established when the inter-NCD pillar distance varied between 4 and 9 lm.The findings demonstrate that regenerating auditory neurons show a strong affinity to the NCD pillars, and the technique could be used for neural guidance and the creation of new neural networks. Together with the NCD’s unique anti-bacterial and electrical properties, patterned NCD surfaces could provide designed neural/electrode interfaces to create independent electrical stimulation signals in CI electrode arrays for the neural population.
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| 28. |
- Cao, Huiming, et al.
(författare)
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Effect of Enterohepatic Circulation on the Accumulation of Per- and Polyfluoroalkyl Substances : Evidence from Experimental and Computational Studies
- 2022
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Ingår i: Environmental Science and Technology. - : American Chemical Society (ACS). - 0013-936X .- 1520-5851. ; 56:5, s. 3214-3224
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Tidskriftsartikel (refereegranskat)abstract
- The pharmacokinetic characteristics of per- and polyfluoroalkyl substances (PFAS) affect their distribution and bioaccumulation in biological systems. The enterohepatic circulation leads to reabsorption of certain chemicals from bile back into blood and the liver and thus influences their elimination, yet its influence on PFAS bioaccumulation remains unclear. We explored the role of enterohepatic circulation in PFAS bioaccumulation by examining tissue distribution of various PFAS in wild fish and a rat model. Computational models were used to determine the reabsorbed fractions of PFAS by calculating binding affinities of PFAS for key transporter proteins of enterohepatic circulation. The results indicated that higher concentrations were observed in blood, the liver, and bile compared to other tissues for some PFAS in fish. Furthermore, exposure to a PFAS mixture on the rat model showed that the reabsorption phenomenon appeared during 8-12 h for most long-chain PFAS. Molecular docking calculations suggest that PFAS can bind to key transporter proteins via electrostatic and hydrophobic interactions. Further regression analysis adds support to the hypothesis that binding affinity of the apical sodium-dependent bile acid transporter is the most important variable to predict the human half-lives of PFAS. This study demonstrated the critical role of enterohepatic circulation in reabsorption, distribution, and accumulation of PFAS.
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| 29. |
- Chang, Haigang, et al.
(författare)
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Continuous High Frequency Deep Brain Stimulation of the Rat Anterior Insula Attenuates the Relapse Post Withdrawal and Strengthens the Extinction of Morphine Seeking
- 2020
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Ingår i: Frontiers in Psychiatry. - : Frontiers Media SA. - 1664-0640. ; 11
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Tidskriftsartikel (refereegranskat)abstract
- Deep brain stimulation (DBS) modulates the neuronal activity in specific brain circuits and has been recently considered as a promising intervention for refractory addiction. The insula cortex is the hub of interoception and is known to be involved in different aspects of substance use disorder. In the present study, we investigate the effects of continuous high frequency DBS in the anterior insula (AI) on drug-seeking behaviors and examined the molecular mechanisms of DBS action in morphine-addicted rats. Sprague-Dawley rats were trained to the morphine-conditioned place preference (CPP, day 1-8) followed by bilaterally implanted with DBS electrodes in the AI (Day 10) and recovery (Day 10-15). Continuous high-frequency (HF) -DBS (130 Hz, 150 mu A, 90 mu s) was applied during withdrawal (Day 16-30) or extinction sessions. CPP tests were conducted on days 16, 30, 40 during withdrawal session and several rats were used for proteomic analysis on day 30. Following the complete extinction, morphine-CPP was reinstated by a priming dose of morphine infusion (2 mg/kg). The open field and novel objective recognition tests were also performed to evaluate the DBS side effect on the locomotion and recognition memory. Continuous HF-DBS in the AI attenuated the expression of morphine-CPP post-withdrawal (Day 30), but morphine addictive behavior relapsed 10 days after the cessation of DBS (Day 40). Continuous HF-DBS reduced the period to full extinction of morphine-CPP and blocked morphine priming-induced recurrence of morphine addiction. HF-DBS in the AI had no obvious effect on the locomotor activity and novel objective recognition and did not cause anxiety-like behavior. In addition, our proteomic analysis identified eight morphine-regulated proteins in the AI and their expression levels were reversely changed by HF-DBS. Continuous HF-DBS in the bilateral anterior insula prevents the relapse of morphine place preference after withdrawal, facilitates its extinction, blocks the reinstatement induced by morphine priming and reverses the expression of morphine-regulated proteins. Our findings suggest that manipulation of insular activity by DBS could be a potential intervention to treat substance use disorder, although future research is warranted.
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| 30. |
- Chen, Cheng, et al.
(författare)
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Performance of multiple satellite precipitation estimates over a typical arid mountainous area of China : Spatiotemporal patterns and extremes
- 2020
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Ingår i: Journal of Hydrometeorology. - 1525-755X. ; 21:3, s. 533-550
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Tidskriftsartikel (refereegranskat)abstract
- Precipitation in arid mountainous areas is characterized by low rainfall intensity and large spatial heterogeneity, which challenges satellite-based monitoring by the spaceborne sensors. This study aims to comparatively evaluate the detection ability of spatiotemporal patterns and extremes of rainfall by a range of mainstream satellite precipitation products [TMPA, Climate Hazards Group Infrared Precipitation with Station Data (CHIRPS), and PERSIANN-Climate Data Record (PERSIANN-CDR)] over a typical arid mountainous basin of China, benchmarking against rain gauge data from 2000 to 2015. Results showed that satellite precipitation estimates had relatively low accuracy at the daily scale, while a significant improvement of correlation coefficient (CC;>0.6) and a significant reduction of relative root-mean-square error (RRMSE;, 1.0) were found as time scale increases beyond the monthly scale. CHIRPS tended to overestimate the gauge precipitation with positive relative bias (RB), while the negative RBvalues for TMPAand PERSIANN-CDR indicated there was an underestimation. CHIRPS had the most similar spatial pattern and slope trends of the seasonal precipitation and interannual variations of annual precipitation with gauge observations. With the increase in rainfall rates, the probability of detection (POD) and critical success index (CSI) were reduced and the false alarm ratio (FAR) was increased significantly, demonstrating the limited capability for all the three satellite products for detecting heavy rainfall events. CHIRPS showed the best performance in detecting rainfall extremes compared to TMPA and PERSIANN-CDR, evidenced by the largerCSI values and similar extreme rainfall indices obtained from gauge records. This study provides valuable guidance for choosing satellite precipitation products instead of gauge observations for rainfall monitoring (especially rainfall extremes) and agricultural production management over arid mountainous area.
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| 31. |
- Chen, Cheng, et al.
(författare)
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Triple collocation-based error estimation and data fusion of global gridded precipitation products over the Yangtze River basin
- 2022
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Ingår i: Journal of Hydrology. - : Elsevier BV. - 0022-1694. ; 605
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Tidskriftsartikel (refereegranskat)abstract
- Error estimation and data fusion are critical to improving the accuracy of global model- and satellite-based precipitation products for practical applications. However, they face challenges over vast areas of the world due to limited ground observations. Triple collocation (TC) method can overcome this limitation and provide an efficient way for error estimation without the “ground truth” and thus also for data fusion, by leveraging multi-source observations and model outputs, which have been increasingly available in recent years. In this work, we conducted a comprehensive study on error estimation and data fusion of a number of global gridded precipitation products over the Yangtze River basin from 2015 to 2018 using TC and multiplicative TC (MTC) methods. We use three satellite-based precipitation products such as the IMERG Final (IMERG-F), PERSIANN-CDR (PCDR) and SM2RAIN-ASCAT (SM2R), and one reanalysis dataset ERA5 which contains precipitation estimates. They were grouped into two TC triplets based on different combinations: IMERG-F + SM2R + ERA5 and PCDR + SM2R + ERA5. For performance evaluation, the TC-based error estimation methods were compared to the traditional method using rain gauge data, and the TC-based data fusion methods were compared with two widely-used data fusion methods Bayesian Model Averaging (BMA) and Random Forest based MErging Procedure (RF-MEP). Results showed that ERA5 had the best performance with the largest correlation coefficient (CC, 0.435), while PCDR had the worst accuracy with the smallest CC (0.304) and the largest absolute relative bias (RB, 0.365). TC tended to underestimate the root mean square error (RMSE) with respect to the traditional gauged-based method, but MTC showed a consistent result owing to the employment of a multiplicative error model. The performance of TC-based data fusion methods had no significant difference from BMA and RF-MEP. All data fusion results were better than the original triplets, as the mean CC value increased from 0.38 to 0.47 and the mean RMSE decreased from 15.0 to 13.5 mm/day. In addition, we found that the zero value replacement in MTC had great influence on error estimation, while had limited impacts on data fusion.
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| 32. |
- Chen, Guang, et al.
(författare)
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Dynamic analysis of the effect of nose length on train aerodynamic performance
- 2019
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Ingår i: Journal of Wind Engineering and Industrial Aerodynamics. - : Elsevier BV. - 0167-6105. ; 184, s. 198-208
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Tidskriftsartikel (refereegranskat)abstract
- The improved delayed detached eddy simulation (IDDES) was used to study the influence of the train’s nose length on its aerodynamic performance. Both the time-averaged and instantaneous near-wake structures and the associated distribution of slipstream velocity are compared for three nose lengths. As the nose length increases, the mean and Std values of the drag and lift force are decreased. The shorter nose-length case results in a higher slipstream velocity. In particular, at the TSI track-side position, the TSI value U_2δ for the 5-m nose length case is 30% and 32% higher than the corresponding values for the 7.5-m and 10-m nose length cases, respectively. The dynamical flow topology in the wake reveals that the flow structures of the 5-m nose length are different from those of the other two cases in the tail streamline surface. As nose length increases, the longitudinal vortices are weaker, and the angle and distance between the longitudinal vortices are smaller. The shear production from the P_xy caused by the separation of the boundary layer at the lateral wall of the tail train is greater than that of the P_xz caused by the separation of the boundary layer at the top and bottom of the tail train.
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| 33. |
- Chen, Zhe, et al.
(författare)
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Toward FPGA Security in IoT : A New Detection Technique for Hardware Trojans
- 2019
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Ingår i: IEEE Internet of Things Journal. - : IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC. - 2327-4662. ; 6:4, s. 7061-7068
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Tidskriftsartikel (refereegranskat)abstract
- Nowadays, field programmable gate array (FPGA) has been widely used in Internet of Things (IoT) since it can provide flexible and scalable solutions to various IoT requirements. Meanwhile, hardware Trojan (HT), which may lead to undesired chip function or leak sensitive information, has become a great challenge for FPGA security. Therefore, distinguishing the Trojan-infected FPGAs is quite crucial for reinforcing the security of IoT. To achieve this goal, we propose a clock-tree-concerned technique to detect the HTs on FPGA. First, we present an experimental framework which helps us to collect the electromagnetic (EM) radiation emitted by FPGA clock tree. Then, we propose a Trojan identifying approach which extracts the mathematical feature of obtained EM traces, i.e., 2-D principal component analysis (2DPCA) in this paper, and automatically isolates the Trojan-infected FPGAs from the Trojan-free ones by using a BP neural network. Finally, we perform extensive experiments to evaluate the effectiveness of our method. The results reveal that our approach is valid in detecting HTs on FPGA. Specifically, for the trust-hub benchmarks, we can find out the FPGA with always on Trojans (100% detection rate) while identifying the triggered Trojans with high probability (by up to 92%). In addition, we give a thorough discussion on how the experimental setup, such as probe step size, scanning area, and chip ambient temperature, affects the Trojan detection rate.
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| 34. |
- Chirico, Nicola, et al.
(författare)
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In silico approaches for the prediction of the breakthrough of organic contaminants in wastewater treatment plants
- 2024
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Ingår i: Environmental Science. - 2050-7887 .- 2050-7895. ; 26:2, s. 400-410
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Tidskriftsartikel (refereegranskat)abstract
- The removal efficiency (RE) of organic contaminants in wastewater treatment plants (WWTPs) is a major determinant of the environmental impact of chemicals which are discharged to wastewater. In a recent study, non-target screening analysis was applied to quantify the percentage removal efficiency (RE%) of more than 300 polar contaminants, by analyzing influent and effluent samples from a Swedish WWTP with direct injection UHPLC-Orbitrap-MS/MS. Based on subsets extracted from these data, we developed quantitative structure–property relationships (QSPRs) for the prediction of WWTP breakthrough (BT) to the effluent water. QSPRs were developed by means of multiple linear regression (MLR) and were selected after checking for overfitting and chance relationships by means of bootstrap and randomization procedures. A first model provided good fitting performance, showing that the proposed approach for the development of QSPRs for the prediction of BT is reasonable. By further populating the dataset with similar chemicals using a Tanimoto index approach based on substructure count fingerprints, a second QSPR indicated that the prediction of BT is also applicable to new chemicals sufficiently similar to the training set. Finally, a class-specific QSPR for PEGs and PPGs showed BT prediction trends consistent with known degradation pathways.
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| 35. |
- Clarke, Andrew J., et al.
(författare)
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Non-fullerene acceptor photostability and its impact on organic solar cell lifetime
- 2021
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Ingår i: Cell Reports Physical Science. - : Elsevier. - 2666-3864. ; 2:7
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Tidskriftsartikel (refereegranskat)abstract
- The development of non-fullerene acceptors (NFAs) has facilitated the realization of efficient organic solar cells (OSCs) with minimal burn-in losses and excellent long-term stability. However, the role of NFA molecular structures on device stability remains unclear, limiting commercialization of NFA-based OSCs. Herein, the photostability of 10 OSC devices, fabricated with various NFAs (O-IDTBR, EH-IDTBR, ITIC, and ITIC-M) blended with donor polymers (PTB7-Th, PffBT4T-2OD, and PBDB-T), is investigated. O-IDTBR and EH-IDTBR form highly stable devices with all three polymers, whereas ITIC and ITIC-M devices suffer from burn-in losses and long-term degradation. Conformational instability is found to be responsible for the poor photostability of ITIC and ITIC-M, resulting in poor device stability. Twisting and potential breakage of the chemical bond that links the end group to the main backbone of ITIC and ITIC-M molecules causes undesirable conformational changes. Potential strategies to overcome such detrimental photo-induced conformational changes in NFAs are proposed.
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| 36. |
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| 37. |
- Coll, Claudia, et al.
(författare)
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Association between Aquatic Micropollutant Dissipation and River Sediment Bacterial Communities
- 2020
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Ingår i: Environmental Science and Technology. - : American Chemical Society (ACS). - 0013-936X .- 1520-5851. ; 54:22, s. 14380-14392
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Tidskriftsartikel (refereegranskat)abstract
- Assessment of micropollutant biodegradation is essential to determine the persistence of potentially hazardous chemicals in aquatic ecosystems. We studied the dissipation half-lives of 10 micropollutants in sediment–water incubations (based on the OECD 308 standard) with sediment from two European rivers sampled upstream and downstream of wastewater treatment plant (WWTP) discharge. Dissipation half-lives (DT50s) were highly variable between the tested compounds, ranging from 1.5 to 772 days. Sediment from one river sampled downstream from the WWTP showed the fastest dissipation of all micropollutants after sediment RNA normalization. By characterizing sediment bacteria using 16S rRNA sequences, bacterial community composition of a sediment was associated with its capacity for dissipating micropollutants. Bacterial amplicon sequence variants of the genera Ralstonia, Pseudomonas, Hyphomicrobium, and Novosphingobium, which are known degraders of contaminants, were significantly more abundant in the sediment incubations where fast dissipation was observed. Our study illuminates the limitations of the OECD 308 standard to account for variation of dissipation rates of micropollutants due to differences in bacterial community composition. This limitation is problematic particularly for those compounds with DT50s close to regulatory persistence criteria. Thus, it is essential to consider bacterial community composition as a source of variability in regulatory biodegradation and persistence assessments.
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| 38. |
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| 39. |
- Gao, Feng, et al.
(författare)
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Trap-Induced Losses in Hybrid Photovoltaics
- 2014
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Ingår i: ACS Nano. - : American Chemical Society. - 1936-0851 .- 1936-086X. ; 8:4, s. 3213-3221
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Tidskriftsartikel (refereegranskat)abstract
- We investigate the loss mechanisms in hybrid photovoltaics based on blends of poly(3-hexylthiophene) with CdSe nanocrystals of various sizes. By combining the spectroscopic and electrical measurements on working devices as well as films, we identify that high trap-mediated recombination is responsible for the loss of photogenerated charge carriers in devices with small nanocrystals. In addition, we demonstrate that the reduced open-circuit voltage for devices with small nanocrystals is also caused by the traps.
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| 40. |
- Goss, Matthew, et al.
(författare)
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A simple field-based biodegradation test shows pH to be an inadequately controlled parameter in laboratory biodegradation testing
- 2020
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Ingår i: Environmental Science. - : Royal Society of Chemistry (RSC). - 2050-7887 .- 2050-7895. ; 22:4, s. 1006-1013
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Tidskriftsartikel (refereegranskat)abstract
- Biodegradation tests are essential for characterizing the behavior of organic micropollutants in the environment, but they are carried out almost exclusively in the laboratory. Test parameters such as temperature and test chemical concentration are often applied in ways that affect observed biodegradation, and laboratory testing requires sophisticated temperature-controlled facilities. We developed a field-based test based on OECD 309 which minimizes the need for laboratory resources such as temperature-controlled facilities by using bottles incubated in the natural water body. The test also utilized contaminant residues present in unspiked natural water to increase the relevance of the results to the local system. A test in a local river and a matching lab-based test were conducted in parallel. We quantified 26 of 40 targeted micropollutants and observed dissipation for 13. Significant differences in half-life (up to a factor of 3.5) between lab and field bottles were observed for 7 compounds, with 6 of 7 degrading more slowly in field bottles. For 4 of these, dissipation was positively correlated to the neutral fraction of the chemical. Differences in the neutral fraction arose due to a higher pH in the lab bottles induced by outgassing of CO2 from the oversaturated river water. We conclude that pH is an important parameter to control in biodegradation testing and that field-based tests may be more environmentally relevant.
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| 41. |
- Guo, Shize, et al.
(författare)
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Securing IoT Space via Hardware Trojan Detection
- 2020
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Ingår i: IEEE Internet of Things Journal. - : IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC. - 2327-4662. ; 7:11, s. 11115-11122
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Tidskriftsartikel (refereegranskat)abstract
- Hardware Trojan (HT) is a malicious modification in the chip circuitry, which may lead to undesired chip function changing or sensitive information leaking once activated. As recently studied, HT has become one of the main threats for Internet-of-Things (IoT) security, and therefore, protecting IoT against the HT attack attracts growing attention from IoT researchers. In this article, we propose an HT detection technique which makes use of chip temporal thermal information and self-organizing map (SOM) neural network to automatically isolate the Trojan-infected chips with the Trojan-free ones, and meanwhile, confirm the Trojan location at the infected chips. The experimental results reveal that our method is effective. Specifically, for the Trust-hub benchmarks, it can detect HTs which increase only 0.02% power consumption of the original design and localize the Trojan positions precisely without any error. In addition, we demonstrate the advantages of our method over two existing HT detection methods, namely, the thermal and power map (TPM) and ring oscillator net (RON), and make a thorough discussion on how the thermal image resolution, chip technology, and clustering algorithm affect the Trojan detection results.
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| 42. |
- Guo, Xiaoxian, et al.
(författare)
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Understand the genomic diversity and evolution of fungal pathogen Candida glabrata by genome-wide analysis of genetic variations
- 2020
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Ingår i: Methods. - : Elsevier BV. - 1095-9130 .- 1046-2023. ; 176, s. 82-90
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Tidskriftsartikel (refereegranskat)abstract
- The yeast Candida glabrata, an opportunistic human fungal pathogen, is the second most prevalent cause of candidiasis worldwide, with an infection incidence that has been increasing in the past decades. The completion of the C. glabrata reference genome made fundamental contributions to the understanding of the molecular basis of its pathogenic phenotypes. However, knowledge of genome-wide genetic variations among C. glabrata strains is limited. In this study, we present a population genomic study of C. glabrata based on whole genome re-sequencing of 47 clinical strains to an average coverage of ∼63×. Abundant genetic variations were identified in these strains, including single nucleotide polymorphisms (SNPs), small insertion/deletions (indels) and copy number variations (CNVs). The observed patterns of variations revealed clear population structure of these strains. Using population genetic tests, we detected fast evolution of several genes involved in C. glabrata adherence ability, such as EPA9 and EPA10. We also located genome structural variations, including aneuploidies and large fragment CNVs, in regions that are functionally related to virulence. Subtelometric regions were hotspots of CNVs, which may contribute to variation in expression of adhesin genes that are important for virulence. We further conducted a genome-wide association study that identified two SNPs in the 5′UTR region of CST6 that were associated with fluconazole susceptibility. These observations provide convincing evidence for the highly dynamic nature of the C. glabrata genome with potential adaptive evolution to clinical environments, and offer valuable resources for investigating the mechanisms underlying drug resistance and virulence in this fungal pathogen. (249 words)
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| 43. |
- Hammoud, Hayma, et al.
(författare)
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Insulin differentially modulates GABA signalling in hippocampal neurons and, in an age-dependent manner, normalizes GABA-activated currents in the tg-APPSwe mouse model of Alzheimer's disease
- 2021
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Ingår i: Acta Physiologica. - : John Wiley & Sons. - 1748-1708 .- 1748-1716. ; 232:2
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Tidskriftsartikel (refereegranskat)abstract
- AimWe examined if tonic γ‐aminobutyric acid (GABA)‐activated currents in primary hippocampal neurons were modulated by insulin in wild‐type and tg‐APPSwe mice, an Alzheimer’s disease (AD) model.MethodsGABA‐activated currents were recorded in dentate gyrus (DG) granule cells and CA3 pyramidal neurons in hippocampal brain slices, from 8‐10 weeks old (young) wild‐type mice and in dorsal DG granule cells in adult, 5‐6 and 10‐12 (aged) months old wild‐type and tg‐APPSwe mice, in the absence or presence of insulin, by whole‐cell patch‐clamp electrophysiology.ResultsIn young mice, insulin (1 nM) enhanced the total spontaneous inhibitory postsynaptic current (sIPSCT) density in both dorsal and ventral DG granule cells. The extrasynaptic current density was only increased by insulin in dorsal CA3 pyramidal neurons. In absence of action potentials, insulin enhanced DG granule cells and dorsal CA3 pyramidal neurons miniature IPSCT (mIPSCT) frequency, consistent with insulin regulation of presynaptic GABA release. sIPSCT densities in DG granule cells were similar in wild‐type and tg‐APPSwe mice at 5‐6 months but significantly decreased in aged tg‐APPSwe mice where insulin normalized currents to wild‐type levels. The extrasynaptic current density was increased in tg‐APPSwe mice relative to wild‐type littermates but, only in aged tg‐APPSwe mice did insulin decrease and normalize the current.ConclusionInsulin effects on GABA signalling in hippocampal neurons are selective while multifaceted and context‐based. Not only is the response to insulin related to cell‐type, hippocampal axis‐location, age of animals and disease but also to the subtype of neuronal inhibition involved, synaptic or extrasynaptic GABAA receptors‐activated currents.
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| 44. |
- Han, Fenghui, et al.
(författare)
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Energy analysis and multi-objective optimization of waste heat and cold energy recovery process in LNG-fueled vessels based on a triple organic Rankine cycle
- 2019
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Ingår i: Energy Conversion and Management. - : Elsevier BV. - 0196-8904. ; 195, s. 561-572
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Tidskriftsartikel (refereegranskat)abstract
- Due to the high level of pollutant emissions from traditional marine diesel engines, Liquefied Natural Gas (LNG) as clean energy is becoming a better choice for main engines to replace the traditional fuels. Meanwhile, in order to improve the energy efficiency of the marine power system, the Organic Rankine Cycle (ORC) has been regarded as the most suitable solution to recover the waste heat for the power generation of vessels. In this paper, both the waste heat of the main engine and the cold energy of LNG have been fully considered, and a novel triple ORC process has been proposed for the waste heat and cold energy recovery of LNG-fueled vessels. It adopts the exhaust gas of the main engine and the cooling water from the engine jacket as heat sources, and uses the cold energy of LNG and the sea water as cold sources. Based on the 15 optional working fluid conditions, the heat source utilization rate, system exergy efficiency, net output power, and system cost are, respectively, combined as two objectives, and the multi-objective adaptive firefly algorithm is used to optimize the thermodynamic performance of the system. The optimization results of different heat and cold sources as well as the design parameters have been discussed. Finally, the system's exergy loss has been analyzed to make suggestions for further improvement. The results show that this novel ORC system can better meet the energy recovery requirements of LNG-fueled vessels, with higher net output power, lower cost, and greater energy recovery efficiency. The largest exergy loss of the system exists in the condensers of the stages 2 and 3, and the expanders in the various stages. Therefore, subsequent cooling energy recovery and the use of Stirling engines can be considered to further improve the system efficiency.
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| 45. |
- He, Qinglong, et al.
(författare)
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Additive manufacturing of dense zirconia ceramics by fused deposition modeling via screw extrusion
- 2021
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Ingår i: Journal of the European Ceramic Society. - : Elsevier BV. - 0955-2219 .- 1873-619X. ; 41:1, s. 1033-1040
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Tidskriftsartikel (refereegranskat)abstract
- A fused deposition modeling(FDM)system via screw extrusion suitable for feeding granular feedstocks with high solid loading was developed. Key parameters included aspect ratio of the screw, the compression ratio and pitch etc. In order to get constant extruded mass flow and wire diameter a processing window was determined by optimizing the barrel temperature, the nozzle diameter and the screwing speed. Microstructural characterization coupled with flexural strength measurement revealed that a higher printing temperature was beneficial to the inter layer bonding. The sintered zirconia ceramic samples with 99% of theoretical density of the 3 mol% yttria stabilized tetragonal zirconia polycrystal (3Y-TZP) and flexural strength of 890 +/- 60 MPa was obtained. A set of zirconia ceramic parts with complex geometries and controllable dimensional accuracy was also successfully prepared for demonstrating the potential of the technique.
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| 46. |
- Hsu, Hsu-Cheng, et al.
(författare)
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Polarized and diameter-dependent Raman scattering from individual aluminum nitride nanowires: The antenna and cavity effects
- 2012
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Ingår i: Applied Physics Letters. - : American Institute of Physics (AIP). - 0003-6951 .- 1077-3118. ; 101:12, s. 121902-
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Tidskriftsartikel (refereegranskat)abstract
- Raman scattering of individual aluminum nitride (AlN) nanowires is investigated systematically. The axial direction of single nanowire can be rapidly verified by polarized Raman scattering. The angular dependencies of E-2(high) mode show strongly anisotropic behavior in smaller nanowires, which results from optical antenna effect. Raman enhancement (RE) per unit volume of E-2(high) increases with decreasing diameter of nanowires. Compared to the thin film, similar to 200-fold increase of RE is observed in AlN nanowires having diameter less than 50 nm, which is far beyond the quantum confinement regime. Such a large RE can be attributed to the effects of resonant cavity and stimulated Raman scattering.
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| 47. |
- Hu, Wenxiu, et al.
(författare)
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High-Dimensional Feature Based Non-Coherent Detection for Multi-Intensity Modulated Ultraviolet Communications
- 2022
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Ingår i: Journal of Lightwave Technology. - : Institute of Electrical and Electronics Engineers (IEEE). - 0733-8724 .- 1558-2213. ; 40:7, s. 1879-1887
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Tidskriftsartikel (refereegranskat)abstract
- Ultraviolet communication (UVC) has been regarded as a promising supplement for overloaded conventional wireless communications. One challenge lies in the communication deterioration caused by the UV-photon scattering induced intersymbol-interference (ISI), which will be even worse when encountering multilevel pulse amplitude modulation (multi-PAM) symbols. To address this ISI, traditional coherent detection methods (e.g., maximum-likelihood sequence detection, MLSD) require high computational complexities for UV channel estimation and sequential detection space formation, thereby making them less attractive. Current non-coherent detection, which simply combines the ISI-insensitive UV signal features (e.g., the rising edge) into a one-dimensional (1D) metric, cannot guarantee reliable communication accuracy. In this work, a novel high-dimensional (HD) non-coherent detection scheme is proposed, leveraging a HD construction of the ISI-insensitive UV signal features. By doing so, we transform the ISI caused sequential detection into an ISI-released HD detection framework, which avoids complex channel estimation and sequential detection space computation. Then, to compute the detection surface, a UV feature based unsupervised learning approach is designed. We deduce the theoretical bit error rate (BER), and prove that the proposed HI) non-coherent detection method has a lower BER than that of the current 1D non-coherent scheme. Simulation results validate our results, and more importantly, demonstrate a BER that approaches that of the state-of-the-art coherent MLSD (<1 dB in SNR at BER = 4.5 x 10(-8), the 7% overhead forward-error-correction limit), and also a reduction of computational complexity by at least two orders of magnitude.
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| 48. |
- Hu, Xiaosong, et al.
(författare)
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Co-estimation of state of charge and state of health for lithium-ion batteries based on fractional-order calculus
- 2018
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Ingår i: IEEE Transactions on Vehicular Technology. - 0018-9545 .- 1939-9359. ; 67:11, s. 10319-10329
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Tidskriftsartikel (refereegranskat)abstract
- Lithium-ion batteries have emerged as the state-of-The-Art energy storage for portable electronics, electrified vehicles, and smart grids. An enabling Battery Management System holds the key for efficient and reliable system operation, in which State-of-Charge (SOC) estimation and State-of-Health (SOH) monitoring are of particular importance. In this paper, an SOC and SOH co-estimation scheme is proposed based on the fractional-order calculus. First, a fractional-order equivalent circuit model is established and parameterized using a Hybrid Genetic Algorithm/Particle Swarm Optimization method. This model is capable of predicting the voltage response with a root-mean-squared error less than 10 mV under various driving-cycle-based tests. Comparative studies show that it improves the modeling accuracy appreciably from its second-and third-order counterparts. Then, a dual fractional-order extended Kalman filter is put forward to realize simultaneous SOC and SOH estimation. Extensive experimental results show that the maximum steady-state errors of SOC and SOH estimation can be achieved within 1%, in the presence of initial deviation, noise, and disturbance. The resilience of the co-estimation scheme against battery aging is also verified through experimentation.
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| 49. |
- Huang, Yuting, et al.
(författare)
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Host-Guest Strategy Enabling Nonhalogenated Solvent Processing for High-Performance All-Polymer Hosted Solar Cells
- 2023
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Ingår i: Chinese journal of chemistry. - : WILEY-V C H VERLAG GMBH. - 1001-604X .- 1614-7065. ; 41:9, s. 1066-1074
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Tidskriftsartikel (refereegranskat)abstract
- The power conversion efficiencies (PCEs) of all-polymer solar cells (all-PSCs), usually processed from low-boiling-point and toxic solvents, have reached high values of 18%. However, poor miscibility and uncontrollable crystallinity in polymer blends lead to a notable drop in the PCEs when using green solvents, limiting the practical development of all-PSCs. Herein, a third component (guest) BTO was employed to optimize the miscibility and enhance the crystallinity of PM6/PY2Se-F host film processed from green solvent toluene (TL), which can effectively suppress the excessive aggregation of PY2Se-F and facilitate a nano-scale interpenetrating network morphology for exciton dissociation and charge transport. As a result, TL-processed all-polymer hosted solar cells (all-PHSCs) exhibited an impressive PCE of 17.01%. Moreover, the strong molecular interaction between the host and guest molecules also enhances the thermal stability of the devices. Our host-guest strategy provides a unique approach to developing high-efficiency and stable all-PHSCs processed from green solvents, paving the way for the industrial development of all-PHSCs.
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| 50. |
- Jin, Cenqin, et al.
(författare)
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High-Speed Long-Haul Multi-Channel Nonlinear Optical Communication Systems Influenced by Equalization Enhanced Phase Noise
- 2022
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Ingår i: 2022 IEEE 14Th International Conference On Advanced Infocomm Technology (ICAIT 2022). - : Institute of Electrical and Electronics Engineers (IEEE). ; , s. 103-106
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Konferensbidrag (refereegranskat)abstract
- In this work, the performance of high-speed long-haul nonlinear Nyquist-spaced multi-channel coherent optical fiber communication systems utilizing electronic dispersion compensation and digital nonlinearity compensation is explored taking into consideration the enhanced equalization phase noise. The analytical model has also been developed to estimate the system performance under different transmission scenarios.
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