| 1. |
- Lindblom, Cecilia, Universitetslektor, 1979-, et al.
(författare)
-
Challenges to Interdisciplinary teaching for nutrition and health in Swedish compulsory schools
- 2020
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Ingår i: International Journal of Home Economics. - Bonn : International Federation for Home Economics. - 1999-561X. ; 13:1, s. 15-29
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Tidskriftsartikel (refereegranskat)abstract
- The Swedish National Agency for Education states that educational provision should involve pupil opportunities for interdisciplinary work and the experience of learning in different ways. In this context, the current study aimed to explore the actual operation of interdisciplinary teaching (IDT) in Swedish compulsory schools, i.e. from preschool (6 years) to grade 9 (16 years) regarding nutrition and health. To investigate this, two web-based nationwide questionnaires were sent out in 2014 to compulsory schools in Sweden. One questionnaire was aimed at teachers in five subjects: Home and Consumer Studies (HCS), Natural Science Subjects (NSS) (Biology, Chemistry, Physics), and Physical Education and Health (PEH). The second questionnaire was for school principals. A total of 388 teachers and 216 principals answered the respective questionnaire. The study showed that 40% of the teachers and 59% of the principals reported that their school worked in an interdisciplinary way regarding nutrition and health education. Practical scheduling problems and a lack of time for planning were seen as the main barriers by both teachers and principals, but to a much larger extent by teachers. A prerequisite for successful IDT is that teachers have a chance to meet and plan, and this study indicates that frame factors have a critical impact on what is possible regarding IDT in Swedish schools. It is crucial that principals appreciate their part in facilitating IDT. Increased interdisciplinary teaching for nutrition and health (IDT-NH) might increase school potential for the better integration of knowledge and understanding about the importance of lifestyle for health, the environment and society.
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| 2. |
- Berg, Gita, 1982-, et al.
(författare)
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Sustainable Home Economics Teacher Education in Pandemic Times : Experiences from Swedish Universities
- 2022
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Konferensbidrag (refereegranskat)abstract
- One aspect of the Sustainable Development Goals is quality education. Not only should education work in favor of sustainable development on a global level; quality education needs to be sustainable in the sense that it provides opportunities for students’ learning in relation to the subject taught. The school subject Home Economics (HE) is often described as having cooking as a core feature. Consequently, HE-teacher education commonly includes practical cooking as a central part of the content. During the outbreak of the Covid-19 pandemic, educational preconditions suddenly changed, and possibilities for undertaking cooking on campus became limited. In this study, we investigate HE-teacher educators’ experiences of adjusting practical cooking activities during the covid-19 pandemic. The aim is twofold: first, to highlight how the pandemic situation has been tackled in Swedish HE-teacher education. Second, to contribute to the understanding of practical cooking as a part of sustainable HE-teacher education.In Sweden, there are four universities that offer HE-teacher education. Educators from these four universities were interviewed digitally in focus groups in two rounds. The first round was held in October 2020, the second in April/May 2021. The interviews followed a semi-structured design, and questions were asked about what the pandemic had meant for practical cooking activities in terms of problems, solutions and insights for the future. The interviews are analyzed using thematic analysis, and preliminary results show e.g. how educators used digital solutions, making the education accessible despite new restrictions. These solutions did not necessarily affect students’ possibilities to achieve formally stated learning goals, but there was a hesitation concerning whether tacit and situated aspects of learning was lost when physical encounters did not take place. In short, the results evoke new insights regarding what constitutes sustainable education for HE-teachers of today.
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| 3. |
- Bohm, Ingela, et al.
(författare)
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Absence, deviance and unattainable ideals : Discourses on vegetarianism in the Swedish school subject Home and Consumer Studies
- 2016
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Ingår i: Health Education Journal. - : Sage Publications. - 0017-8969 .- 1748-8176. ; 75:6, s. 676-688
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Tidskriftsartikel (refereegranskat)abstract
- Objective: This study aimed to describe Discourses on vegetarian food in the Swedish school subject Home and Consumer Studies. Design: The study involved the observation of naturally occurring classroom talk, with audio recording and in some cases video-taping. Setting: The study was conducted during Home and Consumer Studies lessons in five different northern Swedish schools. Method: Fifty-nine students and five teachers were observed, recorded and in some cases video-taped. The resulting data were analysed with a focus on big 'D' Discourses. Results: Results indicated that gendered Discourses of absence, deviance and unattainability restricted some students' access to vegetarian food. The absence of meat was constructed as simultaneously healthy and unhealthy, a lack of cultural familiarity with vegetarian cooking made finding recipes difficult and students perceived the loss of taste as very negative. The vegetarian was seen as deviant, with vegetarianism being conceptually equated with sickness. Access to meat-free food required a commitment to a vegetarian lifestyle, and this was seen as a sacrifice and as too much work, not only for the individual but also for others. Conclusion: To counteract the restricted access to vegetarian food, Home and Consumer Studies teachers can redesign activities in the subject with the help of critical food literacy. For example, cooking could focus on popular plant-based dishes instead of 'empty' vegetarian themes, all students could be allowed to share vegetarian dishes instead of reserving them for vegetarians, the possibly strict rules of vegetarianism could be relaxed for those who do not wish to commit to them and vegetarian food could be deliberately connected to strength and masculinity. However, this presupposes sufficient economic resources and ample food storage space.
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| 4. |
- Bohm, Ingela, et al.
(författare)
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'Don't give us an assignment where we have to use spinach!' : food choice and discourse in home and consumer studies
- 2016
-
Ingår i: International Journal of Consumer Studies. - : Wiley-Blackwell. - 1470-6423 .- 1470-6431. ; 40:1, s. 57-65
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to describe classroom Discourses about vegetables during the planning, cooking, eating and evaluation of meals in the Swedish school subject Home and Consumer Studies. Fifty-nine students and five teachers were recruited from five northern Swedish villages and towns, and then observed, recorded and in some cases video-taped during lessons that took place between 2010 and 2012. Based on 56 instances of talk about vegetables, four Discourses were identified and related to the three aspects of Belasco's culinary triangle of contradictions: identity, responsibility and convenience. The results indicated that the identity-based sensory and cultural Discourses sometimes clashed with the more responsibility-oriented health and evaluation Discourses. The health Discourse was only used when there was an element of evaluation, with assignments connected to grades. In all other cases, the sensory and cultural Discourses guided vegetable use. Sometimes different sensory or cultural assumptions could clash with each other, for example when the teacher insisted on the use of a specific recipe regardless of a student's taste preferences. Since these preferences did not always harmonize with curricular demands for responsibility, there might be a risk of basing grades on aspects of students' identity. Alternatively, students might feel constrained to argue against their own identity in order to be favourably evaluated. Then again, if teachers always bow to student tastes, this limits their chances of learning about food and physical health. Viewing the dilemma through the lens of the culinary triangle of contradictions may help teachers and researchers develop teaching methods that take all aspects of food choice into account.
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| 5. |
- Bohm, Ingela, et al.
(författare)
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"He just has to like ham" : the centrality of meat in home and consumer studies
- 2015
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Ingår i: Appetite. - : Elsevier. - 0195-6663 .- 1095-8304. ; 95, s. 101-112
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Tidskriftsartikel (refereegranskat)abstract
- This study aimed to describe Discourses on meat in the school subject Home and Consumer Studies in five different northern Swedish schools. Fifty-nine students and five teachers from five different schools were recorded and in some cases video-taped during lessons. Results indicate that meat was seen as central to nutritional health, sensory experience, culture and social relationships. This positive view was challenged by an alternative Discourse where meat was threatening to health, sensory experience and psychological comfort, but this was not strong enough to affect centrality. Even when participants sought to promote the health advantages of reducing meat consumption, the dominant centrality Discourse was strengthened. This implies that the possible tension between physical and psychosocial/emotional health can make the benefits of a reduction difficult both to convey and accept. A form of critical food literacy may help teachers deconstruct the arbitrary power of the centrality Discourse, but it may also strengthen meat-eater identities because the social norms that guide food choice become salient. A redesign of Discourses might facilitate a reduction in meat consumption, but such a paradigm shift is dependent on the development of society as a whole, and can only be briefly touched upon within the limited timeframes and resources of Home and Consumer Studies.
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| 6. |
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| 7. |
- Couch, Fergus J., et al.
(författare)
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Identification of four novel susceptibility loci for oestrogen receptor negative breast cancer
- 2016
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Ingår i: Nature Communications. - : NATURE PUBLISHING GROUP. - 2041-1723. ; 7:11375, s. 1-13
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Tidskriftsartikel (refereegranskat)abstract
- Common variants in 94 loci have been associated with breast cancer including 15 loci with genome-wide significant associations (P<5 x 10(-8)) with oestrogen receptor (ER)-negative breast cancer and BRCA1-associated breast cancer risk. In this study, to identify new ER-negative susceptibility loci, we performed a meta-analysis of 11 genome-wide association studies (GWAS) consisting of 4,939 ER-negative cases and 14,352 controls, combined with 7,333 ER-negative cases and 42,468 controls and 15,252 BRCA1 mutation carriers genotyped on the iCOGS array. We identify four previously unidentified loci including two loci at 13q22 near KLF5, a 2p23.2 locus near WDR43 and a 2q33 locus near PPIL3 that display genome-wide significant associations with ER-negative breast cancer. In addition, 19 known breast cancer risk loci have genome-wide significant associations and 40 had moderate associations (P<0.05) with ER-negative disease. Using functional and eQTL studies we implicate TRMT61B and WDR43 at 2p23.2 and PPIL3 at 2q33 in ER-negative breast cancer aetiology. All ER-negative loci combined account for similar to 11% of familial relative risk for ER-negative disease and may contribute to improved ER-negative and BRCA1 breast cancer risk prediction.
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| 8. |
- Dominguez, Cecilia A, et al.
(författare)
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Variability in C-type lectin receptors regulates neuropathic pain-like behavior after peripheral nerve injury
- 2014
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Ingår i: Molecular Pain. - : SAGE Publications. - 1744-8069. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- INTRODUCTION: Neuropathic pain is believed to be influenced in part by inflammatory processes. In this study we examined the effect of variability in the C-type lectin gene cluster (Aplec) on the development of neuropathic pain-like behavior after ligation of the L5 spinal nerve in the inbred DA and the congenic Aplec strains, which carries seven C-type lectin genes originating from the PVG strain.RESULTS: While both strains displayed neuropathic pain behavior early after injury, the Aplec strain remained sensitive throughout the whole study period. Analyses of several mRNA transcripts revealed that the expression of Interleukin-1β, Substance P and Cathepsin S were more up-regulated in the dorsal part of the spinal cord of Aplec rats compared to DA, indicating a stronger inflammatory response. This notion was supported by flow cytometric analysis revealing increased infiltration of activated macrophages into the spinal cord. In addition, macrophages from the Aplec strain stimulated in vitro displayed higher expression of inflammatory cytokines compared to DA cells. Finally, we bred a recombinant congenic strain (R11R6) comprising only four of the seven Aplec genes, which displayed similar clinical and immune phenotypes as the Aplec strain.CONCLUSION: We here for the first time demonstrate that C-type lectins, a family of innate immune receptors with largely unknown functions in the nervous system, are involved in regulation of inflammation and development of neuropathic pain behavior after nerve injury. Further experimental and clinical studies are needed to dissect the underlying mechanisms more in detail as well as any possible relevance for human conditions.
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| 9. |
- Fischer, Helmut, et al.
(författare)
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Contribution of fungi and bacteria to the formation of dissolved organic carbon from decaying common reed (Phragmites australis)
- 2006
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Ingår i: Archiv für Hydrobiologie. - : Schweizerbart. - 0003-9136. ; 166:1, s. 79-97
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Tidskriftsartikel (refereegranskat)abstract
- We examined release and subsequent utilization of DOC from leaves of common reed (Phragmites australis), a macrophyte which often dominates in shallow lakes and constitutes an important source of dissolved organic carbon (DOC). Leaves were incubated submersed in organic-free water for up to 63 days with natural and manipulated microbial communities. By this, we aimed to demonstrate differential effects of bacteria and fungi on the composition and amount of DOC originating from the leaves. DOC was analyzed by its total amount, spectral properties at wavelengths of 250-500 nm and its composition determined by size exclusion chromatography followed by organic carbon detection. Leaching of DOC was fast and the maximum DOC concentration was reached after 48 h. Mean molecule size increased during the first 14 days of incubation. Later on, humic-like substances accumulated, whereas low- and high-molecular-weight DOC were depleted. The formation of DOC from leaf detritus was strongly influenced by the composition of the microbial community present. Bacteria effectively removed low-molecular-weight DOC and accumulated high-molecular-weight DOC during a 7 day incubation. Leaf-degrading fungi promoted the accumulation of high amounts of intermediate-molecular-weight DOC, but were suppressed by the presence of bacteria. The presence of bacteria and/or fungi thus resulted in contrasting patterns of DOC composition, suggesting functional differences and strong interactions between those two major microbial groups during natural decomposition of leaves. The activity and interactions of both groups may therefore be significant for DOC composition in aquatic systems.
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| 10. |
- Gallini, Radiosa, et al.
(författare)
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PDGF-A and PDGF-B induces cardiac fibrosis in transgenic mice
- 2016
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Ingår i: Experimental Cell Research. - : Elsevier BV. - 0014-4827 .- 1090-2422. ; 349:2, s. 282-290
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Tidskriftsartikel (refereegranskat)abstract
- Platelet-derived growth factors (PDGFs) and their receptors (PDGFRs) contribute to normal heart development. Deficient or abnormal expression of Pdgf and Pdgfr genes have a negative impact on cardiac development and function. The cellular effects of PDGFs in the hearts of Pdgf/Pdgfr mutants and the pathogenesis of the resulting abnormalities are poorly understood, but different PDGF isoforms induce varying effects. Here, we generated three new transgenic mouse types which complete a set of studies, where all different PDGF ligands have been expressed under the same heart specific alpha-myosin heavy chain promoter. Transgenic expression of the natural isoforms of Pdgfa and Pdgfb resulted in isoform specific fibrotic reactions and cardiac hypertrophy. Pdgfa overexpression resulted in a severe fibrotic reaction with up to 8-fold increase in cardiac size, leading to lethal cardiac failure within a few weeks after birth. In contrast, Pdgfb overexpression led to focal fibrosis and moderate cardiac hypertrophy. As PDGF-A and PDGF-B have different affinity for the two PDGF receptors, we analyzed the expression of the receptors and the histology of the fibrotic hearts. Our data suggest that the stronger fibrotic effect generated by Pdgfa overexpression was mediated by Pdgfrα in cardiac interstitial mesenchymal cells, i.e. the likely source of extracellular matrix depostion and fibrotic reaction. The apparent sensitivity of the heart to ectopic PDGFRα agonists supports a role for endogenous PDGFRα agonists in the pathogenesis of cardiac fibrosis. © 2016 The Authors
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| 11. |
- Hollestelle, Antoinette, et al.
(författare)
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No clinical utility of KRAS variant rs61764370 for ovarian or breast cancer
- 2016
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Ingår i: Gynecologic Oncology. - : Elsevier BV. - 0090-8258 .- 1095-6859. ; 141:2, s. 386-401
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Tidskriftsartikel (refereegranskat)abstract
- Objective Clinical genetic testing is commercially available for rs61764370, an inherited variant residing in a KRAS 3′ UTR microRNA binding site, based on suggested associations with increased ovarian and breast cancer risk as well as with survival time. However, prior studies, emphasizing particular subgroups, were relatively small. Therefore, we comprehensively evaluated ovarian and breast cancer risks as well as clinical outcome associated with rs61764370. Methods Centralized genotyping and analysis were performed for 140,012 women enrolled in the Ovarian Cancer Association Consortium (15,357 ovarian cancer patients; 30,816 controls), the Breast Cancer Association Consortium (33,530 breast cancer patients; 37,640 controls), and the Consortium of Modifiers of BRCA1 and BRCA2 (14,765 BRCA1 and 7904 BRCA2 mutation carriers). Results We found no association with risk of ovarian cancer (OR = 0.99, 95% CI 0.94-1.04, p = 0.74) or breast cancer (OR = 0.98, 95% CI 0.94-1.01, p = 0.19) and results were consistent among mutation carriers (BRCA1, ovarian cancer HR = 1.09, 95% CI 0.97-1.23, p = 0.14, breast cancer HR = 1.04, 95% CI 0.97-1.12, p = 0.27; BRCA2, ovarian cancer HR = 0.89, 95% CI 0.71-1.13, p = 0.34, breast cancer HR = 1.06, 95% CI 0.94-1.19, p = 0.35). Null results were also obtained for associations with overall survival following ovarian cancer (HR = 0.94, 95% CI 0.83-1.07, p = 0.38), breast cancer (HR = 0.96, 95% CI 0.87-1.06, p = 0.38), and all other previously-reported associations. Conclusions rs61764370 is not associated with risk of ovarian or breast cancer nor with clinical outcome for patients with these cancers. Therefore, genotyping this variant has no clinical utility related to the prediction or management of these cancers.
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| 12. |
- Höijer, Karin, 1971-, et al.
(författare)
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"Vi har fått syn på hur viktig den praktiska kunskapen är” : Lärarutbildare på fyra lärosäten om att utbilda under pandemin
- 2021
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Ingår i: ABSTRACTS LÄRARLÄRDOM 2021.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Mat och matlagning beskrivs ofta som själva kärnan i skolämnet hem- och konsumentkunskap (HK). Att utbilda nya lärare inom ämnet med för således att praktisk matlagning ingår som en central del. I och med Coronapandemin ändrades förutsättningarna för att bedriva undervisning, vilket påverkade förutsättningarna för undervisning i praktisk matlagning vid de fyra lärosätena som bedriver utbildning av HK-lärare. Vad innebär det för lärarutbildare att bedriva undervisning i praktiska moment under en pandemi?I det här projektet samlades lärarutbildare från Göteborg universitet, Högskolan Kristianstad, Umeå universitet samt Uppsala universitet i fokusgrupper i två omgångar för att berätta vad pandemin inneburit för undervisningen i praktisk matlagning, vilka lösningar som har framkommit och vilka lärdomar man tar med sig in i framtiden. En första intervjuomgång genomfördes i oktober 2020 och uppföljningsintervjuer gjordes under april och maj 2021.Preliminära resultat visade att den praktiska matlagningen inte bara handlade om matlagningsmetoder utan allt det som händer “runt omkring”. De förändrade förutsättningarna påverkade inte nödvändigtvis studenternas förutsättningar att uppnå lärandemålen men det uttrycktes en oro för att den tysta kunskap som ofta genereras i det nära fysiska mötet mellan människor gått förlorad.Denna studie är den första i sitt slag som inkluderar lärare inom högre utbildning för ämnet HK från samtliga utbildningsorter och de preliminära resultaten väcker nya frågeställningar kring lärandemålens formuleringar och deras relevans för framtidens HK-lärare.
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| 13. |
- Höijer, Karin, et al.
(författare)
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"Vi har fått syn på hur viktig den praktiska kunskapen är” : Lärarutbildare på fyra lärosäten om att utbilda under pandemin
- 2021
-
Ingår i: ABSTRACTS LÄRARLÄRDOM 2021.
-
Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Mat och matlagning beskrivs ofta som själva kärnan i skolämnet hem- och konsumentkunskap (HK). Att utbilda nya lärare inom ämnet med för således att praktisk matlagning ingår som en central del. I och med Coronapandemin ändrades förutsättningarna för att bedriva undervisning, vilket påverkade förutsättningarna för undervisning i praktisk matlagning vid de fyra lärosätena som bedriver utbildning av HK-lärare. Vad innebär det för lärarutbildare att bedriva undervisning i praktiska moment under en pandemi? I det här projektet samlades lärarutbildare från Göteborg universitet, Högskolan Kristianstad, Umeå universitet samt Uppsala universitet i fokusgrupper i två omgångar för att berätta vad pandemin inneburit för undervisningen i praktisk matlagning, vilka lösningar som har framkommit och vilka lärdomar man tar med sig in i framtiden. En första intervjuomgång genomfördes i oktober 2020 och uppföljningsintervjuer gjordes under april och maj 2021. Preliminära resultat visade att den praktiska matlagningen inte bara handlade om matlagningsmetoder utan allt det som händer “runt omkring”. De förändrade förutsättningarna påverkade inte nödvändigtvis studenternas förutsättningar att uppnå lärandemålen men det uttrycktes en oro för att den tysta kunskap som ofta genereras i det nära fysiska mötet mellan människor gått förlorad. Denna studie är den första i sitt slag som inkluderar lärare inom högre utbildning för ämnet HK från samtliga utbildningsorter och de preliminära resultaten väcker nya frågeställningar kring lärandemålens formuleringar och deras relevans för framtidens HK-lärare.
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| 14. |
- Jansson, Agneta, 1973-, et al.
(författare)
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A new polymorphism in the coding region of exon four in HSD17B2 in relation to risk of sporadic and hereditary breast cancer
- 2007
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Ingår i: Breast Cancer Research and Treatment. - : Springer Science and Business Media LLC. - 0167-6806 .- 1573-7217. ; 106:1, s. 57-64
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Tidskriftsartikel (refereegranskat)abstract
- In situ synthesis of oestrogens is of great importance in the development and progression of breast cancer. 17β-hydroxysteroid dehydrogenase (17HSD) type 2 catalyses oxidation from oestradiol to oestrone, and thereby protects the breast epithelial cells from oestradiol. Low expression of 17HSD type 2 has been associated with decreased survival in breast cancer, but no studies have investigated the mechanism behind the low expression. The 17HSD type 2 gene (HSD17B2) was screened for mutations with Single Stranded Conformation Polymorphism (SSCP)-DNA sequencing in 59 sporadic breast cancer cases, 19 hereditary breast cancer cases and seven breast cancer cell lines. DNA samples from 226 healthy individuals were used to identify if changes were previously unknown polymorphisms. No mutation was detected and therefore mutations in HSD17B2 do not explain why some breast tumours exhibit low 17HSD type 2 expression. However, a previously unknown polymorphism was found in exon four (Met226Val). Using molecular modelling, we found that the substituted residue is located at the outer part of the steroid binding site, probably causing minor alterations in the substrate binding. We further studied if the polymorphism contributes to breast cancer susceptibility in a larger material, but did not find an increased risk in the group of 317 sporadic breast cancer patients, 188 breast cancer patients with two close relatives with breast cancer or 122 hereditary breast cancer patients, compared to the healthy control group. We suggest that the detected polymorphism does not contribute to a higher risk of developing breast cancer.
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| 15. |
- Kiemeney, Lambertus A, et al.
(författare)
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A sequence variant at 4p16.3 confers susceptibility to urinary bladder cancer.
- 2010
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Ingår i: Nature genetics. - : Springer Science and Business Media LLC. - 1546-1718 .- 1061-4036. ; 42:5, s. 415-9
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Tidskriftsartikel (refereegranskat)abstract
- Previously, we reported germline DNA variants associated with risk of urinary bladder cancer (UBC) in Dutch and Icelandic subjects. Here we expanded the Icelandic sample set and tested the top 20 markers from the combined analysis in several European case-control sample sets, with a total of 4,739 cases and 45,549 controls. The T allele of rs798766 on 4p16.3 was found to associate with UBC (odds ratio = 1.24, P = 9.9 x 10(-12)). rs798766 is located in an intron of TACC3, 70 kb from FGFR3, which often harbors activating somatic mutations in low-grade, noninvasive UBC. Notably, rs798766[T] shows stronger association with low-grade and low-stage UBC than with more aggressive forms of the disease and is associated with higher risk of recurrence in low-grade stage Ta tumors. The frequency of rs798766[T] is higher in Ta tumors that carry an activating mutation in FGFR3 than in Ta tumors with wild-type FGFR3. Our results show a link between germline variants, somatic mutations of FGFR3 and risk of UBC.
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| 16. |
- Lawrenson, Kate, et al.
(författare)
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Functional mechanisms underlying pleiotropic risk alleles at the 19p13.1 breast-ovarian cancer susceptibility locus
- 2016
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Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 7
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Tidskriftsartikel (refereegranskat)abstract
- A locus at 19p13 is associated with breast cancer (BC) and ovarian cancer (OC) risk. Here we analyse 438 SNPs in this region in 46,451 BC and 15,438 OC cases, 15,252 BRCA1 mutation carriers and 73,444 controls and identify 13 candidate causal SNPs associated with serous OC (P=9.2 × 10-20), ER-negative BC (P=1.1 × 10-13), BRCA1-associated BC (P=7.7 × 10-16) and triple negative BC (P-diff=2 × 10-5). Genotype-gene expression associations are identified for candidate target genes ANKLE1 (P=2 × 10-3) and ABHD8 (P<2 × 10-3). Chromosome conformation capture identifies interactions between four candidate SNPs and ABHD8, and luciferase assays indicate six risk alleles increased transactivation of the ADHD8 promoter. Targeted deletion of a region containing risk SNP rs56069439 in a putative enhancer induces ANKLE1 downregulation; and mRNA stability assays indicate functional effects for an ANKLE1 3′-UTR SNP. Altogether, these data suggest that multiple SNPs at 19p13 regulate ABHD8 and perhaps ANKLE1 expression, and indicate common mechanisms underlying breast and ovarian cancer risk.
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| 17. |
- Lindblom, Cecilia, 1979-, et al.
(författare)
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Group work interaction among pupils In Home and Consumer Studies in Sweden
- 2016
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Ingår i: International Journal of Home Economics. - : International Federation for Home Economics. - 1999-561X. ; 9:1, s. 35-53
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to describe and analyze different types of group work during Home and Consumer Studies lessons in four Swedish schools, located in two different municipalities. Observations of pupils' practical tasks were carried out in six classrooms, and video/sound recorded. Study participants totaled 44 pupils (11 – 16 years old). Qualitative content analysis of the observations showed four different types of group work; 1) integrated, 2) expert (appointed by the kitchen partner/s and self-appointed), 3) divided, and 4) parallel. The most important finding was that pupils belonging to a self-appointed expert group, a divided group or a parallel group did not have the best conditions for learning. The integrated type appeared more conducive to individual learning than the other three. As a result, teachers have to be aware that non-functioning group work can negatively impact pupils' possibility of achieving the grade-related tasks for the subject.
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| 18. |
- Lindblom, Cecilia, Universitetslektor, 1979-
(författare)
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Grupparbete i hem- och konsumentkunskap
- 2019. - 1
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Ingår i: Didaktik för hem- och konsumentkunskap. - Malmö : Gleerups Utbildning AB. - 9789151100463 ; , s. 83-94
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 19. |
- Lindblom, Cecilia, 1979-, et al.
(författare)
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Interdisciplinary work regarding nutrition and health in Swedish compulsory school : a survey study
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- The Swedish National Agency for Education state that implementation of education should involve possibilities for pupils to work interdisciplinary and experience knowledge in different ways. The aim of the study was to explore the presence of interdisciplinary work related to the content of nutrition and health in Swedish compulsory school, and factors influencing interdisciplinary work. To investigate this, two web-based nationwide questionnaires were sent out in 2014 to compulsory schools in Sweden. One questionnaire was aimed at teachers in five subjects: Home and Consumer Studies, Physical Education and Health, Biology, Chemistry, and Physics. The second questionnaire was aimed at head teachers. A total of 388 teachers and 216 head teachers answered the respective questionnaires. The study showed that 40% of the teachers and 59% of the head teachers reported that their school worked interdisciplinary regarding nutrition and health. Lack of time for planning and scheduling problems were seen as the main barriers by both teachers and head teachers. A pre-requisite for interdisciplinary work to be successful is that teachers have a chance to meet and plan, and the study indicate that frame factors have a critical impact on what is possible to perform regarding interdisciplinary work in Swedish schools. Improving the interdisciplinary work regarding nutrition and health might increase the potential for schools to achieve the overall goal of pupils gaining knowledge and understanding of the importance of their lifestyle for health, the environment and society.
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| 20. |
- Lindblom, Cecilia, 1979-, et al.
(författare)
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Practical conditions for home and consumer studies in Swedish compulsory education : a survey study
- 2013
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Ingår i: International Journal of Consumer Studies. - : Wiley-Blackwell. - 1470-6423 .- 1470-6431. ; 37:5, s. 556-563
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to map the field of internal and external conditions that Swedish Home and Consumer Studies teachers and pupils have contend with. A questionnaire was constructed and delivered online in November 2010. It contained 27 question covering teacher qualification, quality of premises and equipment, lesson time and collaboration, and the use of national syllabi. A total of 385 persons across the country answered the questionnaire; about 21% of those teaching Home and Consumer Studies in Swedish compulsory school during the school year 2009–2010. These respondents taught in a total of 392 compulsory schools, which equalled about 22% of the schools teaching grades 7–9 in Sweden. Almost a quarter (23%) of the teachers lacked formal training for Home and Consumer Studies. Respondents without an appropriate degree qualification included those trained as nursery school teachers, dietitians and civil engineers. As regards classrooms, while 88% of respondents reported access to fully equipped kitchens with stoves, sinks and work surfaces, 5% used regular classrooms and the remainder were obliged to come up with alternative solutions, such as using portable kitchens in regular classrooms or conducting their lessons in the school restaurant. This study raises many questions about the quality of Home and Consumer Studies provision in a number of schools. The local deficiencies in the nationally decided frame factors for Home and Consumer Studies found by the present study gives us reason to doubt that all pupils achieve the overall learning goals of ‘knowing in practice’ and making informed choices utilizing environmental, economic and health perspectives. This might affect the health and economy of the individuals in the long run, with implications for the national economy and public health. On the basis of these findings, we therefore recommend that current conditions for Home and Consumer Studies and how the subject should operate in schools become a focus for national debate.
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| 21. |
- Lindblom, Cecilia, 1979-
(författare)
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Skolämnet Hem- och konsumentkunskap på 2000-talet : förutsättningar för elevers möjlighet till måluppfyllelse
- 2016
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Background People are faced with a variety of consumer choices every day. If they do not have adequate and appropriate knowledge their choices create a greater risk of negative effects for the environment and the economy, and also for the individual's personal health and economic circumstances. Home and Consumer Studies (HC-studies) provides school pupils with educational opportunities on which to base their choice decisions.Aim The overall aim of this thesis is to describe and analyze the impact of frame factors such as, for example, teachers’ qualifications, classrooms, lesson time, interdisciplinary work and group work on the educational experience, focusing on pupils' opportunities for goal achievement in an HC-studies context.Methods Two different methods were used to obtain data, 1) surveys and 2) classroom observations. One nationwide survey were sent out in 2010 to people working with HC-studies (N=385) and two surveys were sent out in 2014, one to people working with the five subjects HC-studies, Physical Education and Health, Chemistry, Physics and Biology (n=388), and one to head teachers (N=216). All three surveys involved municipal and independent schools. The observations were carried out in four different schools in grades 5, 8 and 9, when pupils worked in the kitchen units and were made using video and audio recording. In total, 44 pupils participated.Results The surveys showed that the HC-studies teacher qualifications were higher in municipality schools compared to independent schools (86% vs 39%). Classrooms with 4 – 10 kitchen units were more common in the municipality schools than independent schools (94% vs 71%). Among teachers in the five subjects HC-studies, Physical Education and Health, Chemistry, Physics and Biology, as well as Head teachers, about half stated that the school they worked in, or had the responsibility for, used interdisciplinary teaching in the area of nutrition and health. Regarding barriers to interdisciplinary teaching, teachers and head teachers both considered a lack of planning time and scheduling to be the biggest problems.Video and audio recordings showed that lesson time, in combination with the amount of content to be delivered during the lesson, led to stressed and insecure pupils, and both teachers and pupils seemed to have a strong focus on the end result; the meal. Some pupils used a variety of strategies to change the cooking in order to have more chance of completing the process on time. Four different ways in which pupils worked together in the kitchen units emerged: 1) integrated, 2) expert, 3) divided, and 4) parallel group work. Integrated group work seemed to provide opportunities for learning because pupils communicated with each other, discussed how they would divide the cooking within the group, who should do what and how they would work so that everyone could take part in the cooking. The other three types of groups did not seem conductive to learning. Many pupils in these groups did not communicate and were not able to show their skills and creativity. It also emerged that, if they got the chance, pupils chose tasks about which they already had some knowledge.Conclusions This thesis highlights various conditions shaping pupil opportunity to reach goals in the subject. The study raises many questions about the extent to which pupils around the country have the same opportunity to reach goals in the HC-studies syllabus.
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| 22. |
- Lindblom, Cecilia, 1979-, et al.
(författare)
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The importance of time frames in Swedish Home and Consumer Studies
- 2016
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Ingår i: International Journal of Consumer Studies. - : John Wiley & Sons. - 1470-6423 .- 1470-6431. ; 40:3, s. 299-308
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to explore how time frames affect pupils and teachers in cooking activities during lessons of different durations for Home and Consumer Studies, with a specific focus on the pupils’ experiences. To investigate this, classroom observations were carried out in 2011 and 2012, with the help of audio and video recordings in three different schools in Sweden and included altogether 22 pupils (18 girls, 4 boys) in grade 9 (15–16 years). Each class was observed during two separate lessons, lasting between 1 h 50 minutes (110 minutes) and 2 h 20 minutes (140 minutes). The six lessons resulted in 12 hours of video recorded time and 80 hours of sound recordings.The study showed that the importance of finishing on time seemed to be ingrained in most pupils in all classes and the end result was perceived as more important than the process of cooking. The study also showed that knowledge of cooking skills and time management seemed important for success in grade-related tasks. Pupils demonstrated different ways of handling the time frames, e.g. using various methods to speed up the cooking process. This study raises important questions about the relationship between national and local time frames and the curriculum aims in Home and Consumer Studies.
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| 23. |
- Lindblom, Rickard P. F., et al.
(författare)
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Complement receptor 2 is up regulated in the spinal cord following nerve root injury and modulates the spinal cord response
- 2015
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Ingår i: Journal of Neuroinflammation. - : Springer Science and Business Media LLC. - 1742-2094. ; 12
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Tidskriftsartikel (refereegranskat)abstract
- Background: Activation of the complement system has been implicated in both acute and chronic states of neurodegeneration. However, a detailed understanding of this complex network of interacting components is still lacking. Methods: Large-scale global expression profiling in a rat F2(DAxPVG) intercross identified a strong cis-regulatory influence on the local expression of complement receptor 2 (Cr2) in the spinal cord after ventral root avulsion (VRA). Expression of Cr2 in the spinal cord was studied in a separate cohort of DA and PVG rats at different time-points after VRA, and also following sciatic nerve transection (SNT) in the same strains. Consequently, Cr2(-/-) mice and Wt controls were used to further explore the role of Cr2 in the spinal cord following SNT. The in vivo experiments were complemented by astrocyte and microglia cell cultures. Results: Expression of Cr2 in naive spinal cord was low but strongly up regulated at 5-7 days after both VRA and SNT. Levels of Cr2 expression, as well as astrocyte activation, was higher in PVG rats than DA rats following both VRA and SNT. Subsequent in vitro studies proposed astrocytes as the main source of Cr2 expression. A functional role for Cr2 is suggested by the finding that transgenic mice lacking Cr2 displayed increased loss of synaptic nerve terminals following nerve injury. We also detected increased levels of soluble CR2 (sCR2) in the cerebrospinal fluid of rats following VRA. Conclusions: These results demonstrate that local expression of Cr2 in the central nervous system is part of the axotomy reaction and is suggested to modulate subsequent complement mediated effects.
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| 24. |
- Lindblom, Rickard P F, et al.
(författare)
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Genetic variability in the rat Aplec C-type lectin gene cluster regulates lymphocyte trafficking and motor neuron survival after traumatic nerve root injury.
- 2013
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Ingår i: Journal of Neuroinflammation. - : Springer Science and Business Media LLC. - 1742-2094. ; 10
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: C-type lectin (CLEC) receptors are important for initiating and shaping immune responses; however, their role in inflammatory reactions in the central nervous system after traumatic injuries is not known. The antigen-presenting lectin-like receptor gene complex (Aplec) contains a few CLEC genes, which differ genetically among inbred rat strains. It was originally thought to be a region that regulates susceptibility to autoimmune arthritis, autoimmune neuroinflammation and infection.METHODS: The inbred rat strains DA and PVG differ substantially in degree of spinal cord motor neuron death following ventral root avulsion (VRA), which is a reproducible model of localized nerve root injury. A large F2 (DAxPVG) intercross was bred and genotyped after which global expressional profiling was performed on spinal cords from F2 rats subjected to VRA. A congenic strain, Aplec, created by transferring a small PVG segment containing only seven genes, all C-type lectins, ontoDA background, was used for further experiments together with the parental strains.RESULTS: Global expressional profiling of F2 (DAxPVG) spinal cords after VRA and genome-wide eQTL mapping identified a strong cis-regulated difference in the expression of Clec4a3 (Dcir3), a C-type lectin gene that is a part of the Aplec cluster. Second, we demonstrate significantly improved motor neuron survival and also increased T-cell infiltration into the spinal cord of congenic rats carrying Aplec from PVG on DA background compared to the parental DA strain. In vitro studies demonstrate that the Aplec genes are expressed on microglia and upregulated upon inflammatory stimuli. However, there were no differences in expression of general microglial activation markers between Aplec and parental DA rats, suggesting that the Aplec genes are involved in the signaling events rather than the primary activation of microglia occurring upon nerve root injury.CONCLUSIONS: In summary, we demonstrate that a genetic variation in Aplec occurring among inbred strains regulates both survival of axotomized motor neurons and the degree of lymphocyte infiltration. These results demonstrate a hitherto unknown role for CLECs for intercellular communication that occurs after damage to the nervous system, which is relevant for neuronal survival.
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| 25. |
- Lindblom, Rickard P. F., et al.
(författare)
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Unbiased expression mapping identifies a link between the complement and cholinergic systems in the rat central nervous system
- 2014
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Ingår i: Journal of Immunology. - : The American Association of Immunologists. - 0022-1767 .- 1550-6606. ; 192:3, s. 1138-1153
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Tidskriftsartikel (refereegranskat)abstract
- The complement system is activated in a wide spectrum of CNS diseases and is suggested to play a role in degenerative phenomena such as elimination of synaptic terminals. Still, little is known of mechanisms regulating complement activation in the CNS. Loss of synaptic terminals in the spinal cord after an experimental nerve injury is increased in the inbred DA strain compared with the PVG strain and is associated with expression of the upstream complement components C1q and C3, in the absence of membrane attack complex activation and neutrophil infiltration. To further dissect pathways regulating complement expression, we performed genome-wide expression profiling and linkage analysis in a large F2(DA × PVG) intercross, which identified quantitative trait loci regulating expression of C1qa, C1qb, C3, and C9. Unlike C1qa, C1qb, and C9, which all displayed distinct coregulation with different cis-regulated C-type lectins, C3 was regulated in a coexpression network immediately downstream of butyrylcholinesterase. Butyrylcholinesterase hydrolyses acetylcholine, which exerts immunoregulatory effects partly through TNF-α pathways. Accordingly, increased C3, but not C1q, expression was demonstrated in rat and mouse glia following TNF-α stimulation, which was abrogated in a dose-dependent manner by acetylcholine. These findings demonstrate new pathways regulating CNS complement expression using unbiased mapping in an experimental in vivo system. A direct link between cholinergic activity and complement activation is supported by in vitro experiments. The identification of distinct pathways subjected to regulation by naturally occurring genetic variability is of relevance for the understanding of disease mechanisms in neurologic conditions characterized by neuronal injury and complement activation.
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| 26. |
- Lönnqvist, Tomas, 1979-, et al.
(författare)
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The potential for waste to biogas in La Paz and El Alto in Bolivia
- 2013
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Ingår i: 1st International Water Association Conference on HolisticSludge Management, 2013, Västerås Sweden.
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Konferensbidrag (refereegranskat)abstract
- In the cities of La Paz and El Alto, 573 tons of organic material are disposed in landfills every day. These residues can be used to produce biogas and recycle nutrients, thus alleviating environmental impacts related to waste management. Technical solutions are evaluated through a multicriteria analysis with the purpose of defining a strategy for implementing waste-to-biogas in the two cities. As a result, the development for waste-to-biogas-system is defined in three steps. Step 1 consists of an active extraction system of landfill gas in the already existing landfills. Step 2 implies the establishment of a dry-digestion biogas facility based on present waste collection practices, that is, not segregated waste. Step 3 consists of a biogas plant using dry digestion for processing source segregated bio-waste. The economic feasibility of these three steps is evaluated. Despite prevailing fossil fuels subsidies in the country, implementing waste-to-biogas turn out feasible in the country provided the digestate is commercialized as bio-fertilizer or erosion control material and additional services such as waste collection and deposition are computed in the total economy of the biogas production plant.
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| 27. |
- Mattsson, C. Mikael, et al.
(författare)
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Träna för en svensk klassiker och andra långlopp
- 2015
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Bok (populärvet., debatt m.m.)abstract
- Förbered dig rätt inför långlopp. Att under ett år genomföra Vätternrundan, Vansbrosimningen, Lidingöloppet och Engelbrektsloppet/Vasaloppet, är ett av de mest populära sätten att skörda frukten av sin träning och nå sitt mål. För att lyckas krävs goda förberedelser, och ju bättre förberedd du är desto mer positiv blir upplevelsen. Innehållet i denna bok är en fördjupning av samlad kunskap och erfarenhet inom simning, cykel, löpning, längdskidåkning och triathlon samt hur man på bästa sätt kombinerar de olika idrotterna – deras olika moment – i träning och tävling. Här hittar du också kapitel om träningslära, idrottsnutrition, mental träning, förebyggande av idrottsskador och träningsplanering för att öka förståelsen av vad som påverkar en idrottsprestation.Extramaterial på webbplatsen: För att kunna erbjuda mer än vad som ryms i en bok, har vi också skapat en webbplats. Med hjälp av koden som följer med i boken kan du registrera dig för att ta del av fördjupande texter, träningsprogram och kostupplägg, övningar och intressanta länkar.(Text från förlaget)
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| 28. |
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| 29. |
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| 30. |
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| 31. |
- Mille-Lindblom, Cecilia, et al.
(författare)
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Ergosterol as a measure of living fungal biomass : persistence in environmental samples after fungal death
- 2004
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Ingår i: Journal of Microbiological Methods. - : Elsevier BV. - 0167-7012. ; 59:2, s. 253-262
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Tidskriftsartikel (refereegranskat)abstract
- The membrane lipid ergosterol is found almost exclusively in fungi, and is frequently used by environmental microbiologists as an indicator of living fungal biomass, based on the assumption that ergosterol is labile, and therefore rapidly degraded after the death of fungal hyphae. We studied the degradation of ergosterol in environmental samples without living fungi. Under the conditions used in this study, ergosterol was very stable both when added as a pure compound and when associated with dead fungi. The decrease of ergosterol was at most 34% during 2 months when protected from sunlight. Presence of a natural bacterial assemblage did not enhance degradation over this time period, as compared to sterile controls. However, photochemical degradation was significant, and led to a 43% decrease of in ergosterol content during 24 h. These results suggest that ergosterol should be used cautiously as a biomarker for living fungi.
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| 32. |
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| 33. |
- Mille-Lindblom, Cecilia, 1974-
(författare)
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Interactions between Bacteria and Fungi on Aquatic Detritus – Causes and Consequences
- 2005
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Bacteria and fungi dominate the decomposition of aquatic plants, a major process in the carbon and nutrient cycling in many aquatic systems. Although phylogenetically distant, bacteria and fungi often live in close proximity with each other. Since these microorganisms also have similar ecological functions, interactions have developed between them. This thesis explores the nature of such interactions, and the potential effects on key components of the decomposition process. The thesis includes a critical assessment of the ergosterol method for determination of fungal biomass, a survey of the environmental factors determining the distribution and taxa numbers of litter-decomposing bacteria and fungi in lakes, and a number of experiments on the interactions between bacteria and fungi. In all the experiments performed, fungi responded to bacterial presence through antagonism, although different fungal strains, bacterial communities and substrates were used. The antagonism seemed to be caused by interference competition for substrate. The fungal effect on bacteria was less consistent. Bacterial growth was suppressed, unaffected, or even enhanced by the presence of fungi. Fungi contributed more to extracellular enzyme production than bacteria, and bacteria were probably able to assimilate intermediate decomposition products formed through the activity of extracellular enzymes of fungal origin. Thus, the effect on bacteria from interacting with fungi was determined by the balance between competition and benefit from excreted enzymes. Bacteria and fungi also used different size fractions of the organic matter, according to their different enzymatic capacities. Hence, bacteria appeared to assimilate low-molecular-weight compounds, while high-molecular-weight compounds were utilized primarily by fungi. In brief, the ecological interactions influenced the growth and hence also the biomass development of bacteria and fungi, which affected enzyme activity as well as utilization of dissolved organic matter. Therefore, I suggest that interactions between bacteria and fungi influence degradation of plant litter in aquatic systems.
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| 34. |
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| 35. |
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| 36. |
- Persson, Johanna, et al.
(författare)
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Can journey mapping be used to visualize information sharing in home care?
- 2022. - 2. ed
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Ingår i: Conference Proceedings of the 51st NES Conference 23-25 October 2022 Uppsala Sweden. - 9789150629750 ; , s. 179-179
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Konferensbidrag (refereegranskat)abstract
- This study investigates the use of journey mapping as a tool for visualizing theflow of information in home care. Journey mapping is a design method with the purpose of visualizing the interaction of a user and a product or service. It tries to encompass the whole user experience including actions and touch points between user and product, feelings and other related information. Hence, the visualized journey in a journey map is originally from one persons’ perspective. Here we will instead emanate from the perspective of the information, and draw the journey map based on different pieces of information, using a set of concrete scenarios from home care. The aim is to get a better understanding of how information flows in the home care setting, and the journey map will be a useful tool in the process of developing home care further. This can for example be in the process of designing digital support systems, for designing the information itself, or for developing work routines around the information.
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| 37. |
- Rafnar, Thorunn, et al.
(författare)
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European genome-wide association study identifies SLC14A1 as a new urinary bladder cancer susceptibility gene.
- 2011
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Ingår i: Human molecular genetics. - : Oxford University Press (OUP). - 1460-2083 .- 0964-6906. ; 20:21, s. 4268-81
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Tidskriftsartikel (refereegranskat)abstract
- Three genome-wide association studies in Europe and the USA have reported eight urinary bladder cancer (UBC) susceptibility loci. Using extended case and control series and 1000 Genomes imputations of 5 340 737 single-nucleotide polymorphisms (SNPs), we searched for additional loci in the European GWAS. The discovery sample set consisted of 1631 cases and 3822 controls from the Netherlands and 603 cases and 37 781 controls from Iceland. For follow-up, we used 3790 cases and 7507 controls from 13 sample sets of European and Iranian ancestry. Based on the discovery analysis, we followed up signals in the urea transporter (UT) gene SLC14A. The strongest signal at this locus was represented by a SNP in intron 3, rs17674580, that reached genome-wide significance in the overall analysis of the discovery and follow-up groups: odds ratio = 1.17, P = 7.6 × 10(-11). SLC14A1 codes for UTs that define the Kidd blood group and are crucial for the maintenance of a constant urea concentration gradient in the renal medulla and, through this, the kidney's ability to concentrate urine. It is speculated that rs17674580, or other sequence variants in LD with it, indirectly modifies UBC risk by affecting urine production. If confirmed, this would support the 'urogenous contact hypothesis' that urine production and voiding frequency modify the risk of UBC.
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| 38. |
- Romaní, Anna Maria, et al.
(författare)
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Interactions of bacteria and fungi on decomposing litter : Differential extracellular enzyme activities
- 2006
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Ingår i: Ecology. - 0012-9658 .- 1939-9170. ; 87:10, s. 2559-2569
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Tidskriftsartikel (refereegranskat)abstract
- Fungi and bacteria are key agents in plant litter decomposition in freshwater ecosystems. However, the specific roles of these two groups and their interactions during the decomposition process are unclear. We compared the growth and patterns of degradative enzymes expressed by communities of bacteria and fungi grown separately and in coexistence on Phragmites leaves. The two groups displayed both synergistic and antagonistic interactions. Bacteria grew better together with fungi than alone. In addition, there was a negative effect of bacteria on fungi, which appeared to be caused by suppression of fungal growth and biomass accrual rather than specifically affecting enzyme activity. Fungi growing alone had a high capacity for the decomposition of plant polymers such as lignin, cellulose, and hemicellulose. In contrast, enzyme activities were in general low when bacteria grew alone, and the activity of key enzymes in the degradation of lignin and cellulose (phenol oxidase and cellobiohydrolase) was undetectable in the bacteria-only treatment. Still, biomass-specific activities of most enzymes were higher in bacteria than in fungi. The low total activity and growth of bacteria in the absence of fungi in spite of apparent high enzymatic efficiency during the degradation of many substrates suggest that fungi provide the bacteria with resources that the bacteria were not able to acquire on their own, most probably intermediate decomposition products released by fungi that could be used by bacteria.
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| 39. |
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| 40. |
- Vrede, Tobias, et al.
(författare)
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Effects of N:P loading ratios on phytoplankton community composition, primary production, and N fixation in a eutrophic lake
- 2009
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Ingår i: Freshwater Biology. - : Wiley. - 0046-5070 .- 1365-2427. ; 54:2, s. 331-344
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Tidskriftsartikel (refereegranskat)abstract
- 1. The aim of this study was to assess the effects of different nitrogen (N) to phosphorus (P) loading ratios on phytoplankton community composition and primary production in a naturally eutrophic lake. Furthermore, the sources of N fuelling primary production were estimated using 15N stable isotope tracers.2. A mesocosm experiment was performed with the same amount of P added to all mesocosms (similar to internal loading rates) but with a range of N additions (0–86 μm N), resulting in a gradient of N : P supply ratios.3. Low N : P supply ratios resulted in a significant shift in the phytoplankton assemblage to a community dominated by N-fixing cyanobacteria and a supply of atmospheric N2 estimated to be up to 60% of total supply.4. The N : P loading ratio had no significant effect on primary production, total nitrogen (TN) concentration or particulate N concentration.5. Our results imply that a reduced N : P ratio of the nutrient load does not necessarily result in a lower TN concentration and downstream N export due to compensation by N-fixing cyanobacteria.
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| 41. |
- Zeng, Chenjie, et al.
(författare)
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Identification of independent association signals and putative functional variants for breast cancer risk through fine-scale mapping of the 12p11 locus
- 2016
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Ingår i: Breast Cancer Research. - : Springer Science and Business Media LLC. - 1465-5411 .- 1465-542X. ; 18
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Tidskriftsartikel (refereegranskat)abstract
- Background: Multiple recent genome-wide association studies (GWAS) have identified a single nucleotide polymorphism (SNP), rs10771399, at 12p11 that is associated with breast cancer risk. Method: We performed a fine-scale mapping study of a 700 kb region including 441 genotyped and more than 1300 imputed genetic variants in 48,155 cases and 43,612 controls of European descent, 6269 cases and 6624 controls of East Asian descent and 1116 cases and 932 controls of African descent in the Breast Cancer Association Consortium (BCAC; http://bcac.ccge.medschl.cam.ac.uk/), and in 15,252 BRCA1 mutation carriers in the Consortium of Investigators of Modifiers of BRCA1/2 (CIMBA). Stepwise regression analyses were performed to identify independent association signals. Data from the Encyclopedia of DNA Elements project (ENCODE) and the Cancer Genome Atlas (TCGA) were used for functional annotation. Results: Analysis of data from European descendants found evidence for four independent association signals at 12p11, represented by rs7297051 (odds ratio (OR) = 1.09, 95 % confidence interval (CI) = 1.06-1.12; P = 3 x 10(-9)), rs805510 (OR = 1.08, 95 % CI = 1.04-1.12, P = 2 x 10(-5)), and rs1871152 (OR = 1.04, 95 % CI = 1.02-1.06; P = 2 x 10(-4)) identified in the general populations, and rs113824616 (P = 7 x 10(-5)) identified in the meta-analysis of BCAC ER-negative cases and BRCA1 mutation carriers. SNPs rs7297051, rs805510 and rs113824616 were also associated with breast cancer risk at P < 0.05 in East Asians, but none of the associations were statistically significant in African descendants. Multiple candidate functional variants are located in putative enhancer sequences. Chromatin interaction data suggested that PTHLH was the likely target gene of these enhancers. Of the six variants with the strongest evidence of potential functionality, rs11049453 was statistically significantly associated with the expression of PTHLH and its nearby gene CCDC91 at P < 0.05. Conclusion: This study identified four independent association signals at 12p11 and revealed potentially functional variants, providing additional insights into the underlying biological mechanism(s) for the association observed between variants at 12p11 and breast cancer risk.
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