| 1. |
- Sheng, Renwang, et al.
(författare)
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Material stiffness in cooperation with macrophage paracrine signals determines the tenogenic differentiation of mesenchymal stem cells
- 2023
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Ingår i: Advanced Science. - : John Wiley & Sons. - 2198-3844. ; 10:17
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Tidskriftsartikel (refereegranskat)abstract
- Stiffness is an important physical property of biomaterials that determines stem cell fate. Guiding stem cell differentiation via stiffness modulation has been considered in tissue engineering. However, the mechanism by which material stiffness regulates stem cell differentiation into the tendon lineage remains controversial. Increasing evidence demonstrates that immune cells interact with implanted biomaterials and regulate stem cell behaviors via paracrine signaling; however, the role of this mechanism in tendon differentiation is not clear. In this study, polydimethylsiloxane (PDMS) substrates with different stiffnesses are developed, and the tenogenic differentiation of mesenchymal stem cells (MSCs) exposed to different stiffnesses and macrophage paracrine signals is investigated. The results reveal that lower stiffnesses facilitates tenogenic differentiation of MSCs, while macrophage paracrine signals at these stiffnesses suppress the differentiation. When exposed to these two stimuli, MSCs still exhibit enhanced tendon differentiation, which is further elucidated by global proteomic analysis. Following subcutaneous implantation in rats for 2 weeks, soft biomaterial induces only low inflammation and promotes tendon-like tissue formation. In conclusion, the study demonstrates that soft, rather than stiff, material has a greater potential to guide tenogenic differentiation of stem cells, which provides comprehensive evidence for optimized bioactive scaffold design in tendon tissue engineering.
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| 2. |
- Liu, Haoyang, et al.
(författare)
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A Novel Vacuum Window System
- 2011
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Ingår i: Proceeding 10<sup>th</sup> International Conference on Sustainable Energy Technologies, Istanbul, Turkey, 04<sup>th</sup> -07<sup>th</sup> September 2011.
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Konferensbidrag (refereegranskat)
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| 3. |
- Zhai, Haoyang, et al.
(författare)
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Anti-ISI Demodulation Scheme and Its Experiment-based Evaluation for Diffusion-based Molecular Communication
- 2018
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Ingår i: IEEE Transactions on Nanobioscience. - : IEEE. - 1536-1241 .- 1558-2639. ; 17:2, s. 126-133
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Tidskriftsartikel (refereegranskat)abstract
- In diffusion-based molecular communication (MC), the most common modulation technique is based on the concentration of information molecules. However, the random delay of molecules due to the channel with memory causes severe inter-symbol interference (ISI) among consecutive signals. In this paper, we propose a detection technique for demodulating signals, the increase detection algorithm (IDA), to improve the reliability of concentration-encoded diffusion-based molecular communication. The proposed IDA detects an increase (i.e., a relative concentration value) in molecule concentration to extract the information instead of detecting an absolute concentration value. To validate the availability of IDA, we establish a real physical tabletop testbed. And we evaluate the proposed demodulation technique using bit error rate (BER) and demonstrate by the tabletop molecular communication platform that the proposed IDA successfully minimizes and even isolates ISI so that a lower BER is achieved than the common demodulation technique.
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| 4. |
- Zhai, Haoyang, et al.
(författare)
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Increase detection algorithm for concentration-encoded diffusion-based molecular communication
- 2017
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Ingår i: Proceedings of the 4th ACM International Conference on Nanoscale Computing and Communication, NanoCom 2017. - New York : ACM Digital Library.
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Konferensbidrag (refereegranskat)abstract
- In this paper, we propose a demodulation technique, the increase detection algorithm (IDA), to improve the reliability of concentration-encoded diffusion-based molecular communication. The proposed demodulation technique detects an increase (i.e., a relative concentration value) in molecule concentration to demodulate information as opposed to detecting an absolute concentration value. We evaluate the proposed demodulation technique in terms of bit error rate (BER) and demonstrate that the proposed demodulation technique successfully isolates ISI from concentration-encoded diffusion-based molecular communication to achieve a lower BER than a commonly used demodulation technique.
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| 5. |
- Zhang, Wei, et al.
(författare)
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Sustained Release of TPCA-1 from Silk Fibroin Hydrogels Preserves Keratocyte Phenotype and Promotes Corneal Regeneration by Inhibiting Interleukin-1β Signaling
- 2020
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Ingår i: Advanced Healthcare Materials. - : Wiley-VCH Verlagsgesellschaft. - 2192-2640 .- 2192-2659. ; 9:17
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Tidskriftsartikel (refereegranskat)abstract
- Corneal injury due to ocular trauma or infection is one of the most challenging vision impairing pathologies that exists. Many studies focus on the pro-inflammatory and pro-angiogenic effects of interleukin-1 beta(IL-1 beta) on corneal wound healing. However, the effect of IL-1 beta on keratocyte phenotype and corneal repair, as well as the underlying mechanisms, is not clear. This study reports, for the first time, that IL-1 beta induces phenotype changes of keratocytes in vitro, by significantly down-regulating the gene and protein expression levels of keratocyte markers (Keratocan, Lumican, Aldh3a1 and CD34). Furthermore, it is found that the NF-kappa B pathway is involved in the IL-1 beta-induced changes of keratocyte phenotype, and that the selective IKK beta inhibitor TPCA-1, which inhibits NF-kappa B, can preserve keratocyte phenotype under IL-1 beta simulated pathological conditions in vitro. By using a murine model of corneal injury, it is shown that sustained release of TPCA-1 from degradable silk fibroin hydrogels accelerates corneal wound healing, improves corneal transparency, enhances the expression of keratocyte markers, and supports the regeneration of well-organized epithelium and stroma. These findings provide insights not only into the pathophysiological mechanisms of corneal wound healing, but also into the potential development of new treatments for patients with corneal injuries.
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| 6. |
- Zhang, Wei, et al.
(författare)
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Tannic acid-mediated dual peptide-functionalized scaffolds to direct stem cell behavior and osteochondral regeneration
- 2020
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Ingår i: Chemical Engineering Journal. - : Elsevier. - 1385-8947 .- 1873-3212. ; 396
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Tidskriftsartikel (refereegranskat)abstract
- The development of cell-instructive scaffolds, which provide biochemical cues to direct endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior, has the potential to revolutionize osteochondral tissue engineering. However, scaffold material itself is generally lacking the inductive signals. Here, a novel peptide-functionalized scaffold was prepared by prime-coating Ca-alginate scaffold with tannic acid (TA) followed by conjugation of E7/P15 peptides (CA-TA-E7/P15). The system leveraged TA as a reactive intermediate between Ca-alginate and peptides due to the multiple functional groups of TA. These interactions induced by TA prime-coating contributed to enhanced scaffold stability and mechanical properties, increased peptide conjugation and sustained release of peptides without affecting their bioactivity, in a TA concentration-dependent manner. The conjugation of E7/P15 peptides endowed the scaffold with the potential to enhance BMSCs recruitment and deposition of cartilage and bone extracellular matrix (ECM). Furthermore, the prepared CA-TA-E7/P15 scaffold showed a promoted biological performance of simultaneous cartilage and subchondral bone regeneration in rabbit osteochondral defect model. These findings indicate that TA is an effective surface modification intermediate and crosslinking aid, and that the CA-TA-E7/P15 scaffold developed in this study serves as a promising cell-instructive scaffold for osteochondral regeneration.
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