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| 4. |
- Eklöw, Annelie, et al.
(författare)
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Three important clones of Listeria monocytogenes
- 1996
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Ingår i: Proceedings of the symposium Food Associated Pathogens, May 6-8, 1996, Uppsala, Sweden. - Uppsala : SLU, Department of Food Hygiene. - 9157651329 ; , s. 221-222
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Konferensbidrag (refereegranskat)abstract
- The object of this study was to characterize Swedish human strains of Listeria monocytogenes
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| 6. |
- Ericsson, Henrik, et al.
(författare)
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An outbreak of listeriosis suspected to have been caused by rainbow trout
- 1997
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Ingår i: Journal of Clinical Microbiology. - : American Society for Microbiology. - 0095-1137 .- 1098-660X. ; 35:11, s. 2904-2907
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Tidskriftsartikel (refereegranskat)abstract
- An outbreak of listeriosis in Sweden, consisting of nine cases, was investigated by means of molecular typing of strains from patients and strains isolated from suspected foodstuffs, together with interviews of the patients. Listeria monocytogenes was isolated from six of the patients, and all isolates were of the same clonal type. This clonal type was also isolated from a 'gravad' rainbow trout, made by producer Y, found in the refrigerator of one of the patients. Unopened packages obtained from producer y were also found to contain the same clonal type of L. monocytogenes. Based on the interview results and the bacteriological typing, we suspect that at least six of the nine cases were caused by gravad or cold-smoked rainbow trout made by producer Y. To our knowledge, this is the first rainbow trout-borne outbreak of listeriosis ever reported.
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| 7. |
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| 8. |
- Loncarevic, Semir, et al.
(författare)
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A case of foodborne listeriosis in Sweden
- 1997
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Ingår i: Letters in Applied Microbiology. - : John Wiley & Sons. - 0266-8254 .- 1472-765X. ; 24:1, s. 65-68
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Tidskriftsartikel (refereegranskat)abstract
- A 70-year-old woman fell seriously ill overnight with meningitis and was admitted to hospital. Cerebrospinal fluid culture yielded Listeria monocytogenes. One of the first problems in solving a human case of listeriosis suspected to be foodborne is to find the foods likely to have been transmitting L. monocytogenes. Two enrichment procedures and a direct plating procedure were used for isolation of the bacteria from different food items collected from the patient's refrigerator, local retail store and producer. Samples of vacuum-packed products of sliced pork brawn, sliced cooked medwurst and berliner wurst of the same brand harboured L. monocytogenes. Serotyping and restriction enzyme analysis (REA) with pulsed-field gel electrophoresis (PFGE) were used to characterize and compare 41 isolates, including the human strain. At least three clones were present in the foods investigated, and one of these was identical to the human clone. This clone was present in samples of medwurst from the patient's refrigerator and the local retail store. This is, to our knowledge, the first proven foodborne case of listeriosis reported in Sweden.
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| 9. |
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| 10. |
- Loncarevic, Semir, et al.
(författare)
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Characterization of Listeria strains isolated from soft and semi-soft cheeses
- 1998
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Ingår i: Food microbiology (Print). - : Elsevier. - 0740-0020 .- 1095-9998. ; 15:5, s. 521-525
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Tidskriftsartikel (refereegranskat)abstract
- Two hundred strains ofListeria monoctyogenespreviously isolated from 19 soft and semi-soft cheeses by enrichment were characterized by serotyping and pulsed-field gel electrophoresis (PFGE). Strains of serogroup 1/2 predominated, with 33.5% of all strains belonging to serovar 1/2a, 58.5% to serovar 1/2b and 5% to serovar 1/2c. By using a PFGE method, 16 different clonal types were obtained. All 10 isolates ofL. monocytogenesrecovered from one white mould cheese were serovar 1/2a, but displayed two different PFGE profiles. Another 10L. monocytogenesisolates from white mould cheese recovered after a 48-h enrichment broth procedure revealed two clonal types, only one of which could be detected after 5 additional days of incubation in the same enrichment broth. Cross-contamination in dairy plants and/or retail stores may play an important role in the incidence and diversity ofL. monocytogenesclonal types recovered from soft and semi-soft cheeses. Therefore, it is necessary to characterize several isolates from the same sample when conducting epidemiological investigations.Copyright © 1998 Academic Press. All rights reserved.
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| 12. |
- Loncarevic, Semir, et al.
(författare)
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Occurrence of Listeria monocytogenes in soft and semi-soft cheeses in retail outlets in Sweden
- 1995
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Ingår i: International Journal of Food Microbiology. - : Elsevier. - 0168-1605 .- 1879-3460. ; 26:2, s. 245-250
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Tidskriftsartikel (refereegranskat)abstract
- Samples of 333 retail cheeses produced in or imported into Sweden were examined for the presence of Listeria monocytogenes. Listeria monocytogenes was isolated from 6% of the cheese samples. Cheeses made from raw milk were more frequently contaminated with L. monocytogenes (42%) than cheeses made from heat-treated milk (2%). The incidence of the organism in whole cheeses and pre-cut wedges was similar (6%). L. monocytogenes was only found in imported cheeses (18 from France, and one from Germany and Italy, respectively). The numbers of L. monocytogenes varied from < 1 x 10(2) to 1 X 10(5) cfu/g. All L. monocytogenes strains belonged to serogroup 1/2, except isolates from two samples that belonged to serogroup 4.
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| 13. |
- Loncarevic, Semir, et al.
(författare)
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Occurrence of Listeria species in an abattoir for cattle and pigs in Bosnia and Hercegovina
- 1994
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Ingår i: Acta Veterinaria Scandinavica. - : Danske Dyrelaegeforening. - 0044-605X .- 1751-0147. ; 35:1, s. 11-15
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Tidskriftsartikel (refereegranskat)abstract
- Altogether 496 samples of meat, lymph nodes, process water and swabs from different places in the abattoir were examined for the presence of Listeria spp. L. monocytogenes was isolated from 31 (6%) and other Listeria spp. from 65 (13%) samples L. monocytogenes was isolated from 2 of 10 beef meat samples, 4 of 50 pig meat samples and 1 of 21 lymph nodes of pigs. No Listeria bacteria were isolated from lymph nodes of cattle. The highest percentage of Listeria was recovered from the unclean sections (cattle 22% and pigs 27%) and the highest frequency was observed during the winter months.
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| 14. |
- Loncarevic, Semir, et al.
(författare)
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Occurrence of Listeria species in broilers pre- and post-chilling in chlorinated water at the two slaughterhouses
- 1994
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Ingår i: Acta Veterinaria Scandinavica. - : Danske Dyrlaegeforening. - 0044-605X .- 1751-0147. ; 35:2, s. 149-154
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Tidskriftsartikel (refereegranskat)abstract
- Altogether 323 pooled samples of neck skins from 1615 broilers from 2 processing plants (A and B) were examined for the presence of Listeria species. The broilers were sampled pre-chilling - after leaving the final rinser but before entering the chiller with chlorinated water - and post-chilling - immediately upon leaving the chiller. Free available chlorine in the chilling water varied from 2 to 15 ppm in plant A and was about 10 ppm in plant B. Listeria monocytogenes was only isolated from broilers in plant A sampled post-chilling (58% of 62 samples). L. innocua was isolated from 19% and 39% of broilers sampled pre-chilling in plants A and B, respectively. Post-chilling, L. innocua was isolated from 3% and 6% of samples from plants A and B, respectively.
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| 15. |
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| 16. |
- Loncarevic, Semir, et al.
(författare)
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Prevalence of Listeria monocytogenes and other Listeria spp. in smoked and ’’gravad’’ fish
- 1996
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Ingår i: Acta Veterinaria Scandinavica. - : Danske Dyrelaegeforening. - 0044-605X .- 1751-0147. ; 37:1, s. 13-18
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Tidskriftsartikel (refereegranskat)abstract
- Altogether 150 samples of vacuum-packed fish were examined for the presence of listeria species. Listeria monocytogenes was isolated from 12 of 58 ''gravad'' fish samples, 3 of 26 cold-smoked and one of 66 hot-smoked fish samples. Ten of these 16 positive samples harboured more than 100 L. monocytogenes cfu/g. The highest level (132 000) was found in a sample of hot-smoked rainbow trout (Oncorhynchus mykiss). Serogroup 1/2 was most frequently found, followed by 4 and 3. One sample of gravad rainbow trout harboured more than one serogroup of L. monocytogenes. L. innocua and L. seeligeri were isolated from 12 and 1 samples, respectively.
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| 17. |
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| 18. |
- Loncarevic, Semir, et al.
(författare)
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The clones of Listeria monocytogenes detected in food depend on the method used
- 1996
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Ingår i: Letters in Applied Microbiology. - : Blackwell Science. - 0266-8254 .- 1472-765X. ; 22:5, s. 381-384
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Tidskriftsartikel (refereegranskat)abstract
- Restriction enzyme analysis (REA) with pulsed-field gel electrophoresis (PFGE) has been used to characterize and compare Listeria monocytogenes strains isolated from foods by two methods, an enrichment procedure and a direct plating procedure. In total 151 isolates from nine foods were investigated, In six of the foods (101 strains investigated) only one clone of L, monocytogenes was found irrespective of the method used. In three foods (50 strains investigated) the direct plating procedure yielded more clones than the enrichment procedure. At the most, five clones were detected in the same food. The results presented here indicate that direct plating from the food reveals more L, monocytogenes clones than revealed by an enrichment procedure.
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| 19. |
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| 20. |
- Tham, Wilhelm, 1951-, et al.
(författare)
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Lessons from an outbreak of listeriosis related to vacuum-packed gravad and cold-smoked fish
- 2000
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Ingår i: International Journal of Food Microbiology. - 0168-1605 .- 1879-3460. ; 62:3, s. 173-175
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Tidskriftsartikel (refereegranskat)abstract
- The first lesson learned from this outbreak was that vacuum-packed rainbow trout is not only an excellent medium for the growth of Listeria monocytogenes, but may also cause human listeriosis. Another lesson is that one single fish processing plant may spread multiple clonal types of L. monocytogenes by selling contaminated products to consumers. Thus, when investigating fish-borne outbreaks of listeriosis one should identify and type several isolates of L. monocytogenes from each food and environmental sample, since multiple clonal types might be present. The outbreak described in this paper involved at least eight human cases, three clonal types of L. monocytogenes, and lasted for 11 months. During the outbreak investigation, L. monocytogenes was also isolated from another brand of rainbow trout found in the refrigerator of one of the patients. These latter isolates belonged to a clonal type not associated with the outbreak. However, this clonal type is of considerable interest since it has been associated with foodborne outbreaks of listeriosis in several countries, and is also the second most common clonal type among human clinical isolates of L. monocytogenes in Sweden. Besides the described outbreak, it is likely that vacuum-packed, cold-smoked and gravad rainbow trout have been involved in additional cases of foodborne listeriosis in Sweden.
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| 21. |
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| 22. |
- Tham, Wilhelm, 1951-, et al.
(författare)
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Swedish human cases of Listeriosis, 1958–1999 : Visulized by LEGO Bricks
- 2000
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Ingår i: Nordic PFGE meeting January 27–28, 2000. Collection of abstracts. Department of Food Hygiene, Faculty of Veterinary Medicine, SLU, Uppsala. - Uppsala : Dep of Food Hygiene, Faculty of Veterinary Medicine, SLU. - 9157659621 ; , s. 27-27
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Konferensbidrag (refereegranskat)
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| 23. |
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