| 1. |
- Allhorn, Maria, et al.
(författare)
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Heme-Scavenging Role of alpha1-Microglobulin in Chronic Ulcers.
- 2003
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Ingår i: Journal of Investigative Dermatology. - : Elsevier BV. - 1523-1747 .- 0022-202X. ; 121:3, s. 640-646
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Tidskriftsartikel (refereegranskat)abstract
- Chronic venous ulcers are characterized by chronic inflammation. Heme and iron, originating from blood cell hemolysis as well as extravascular necrosis, have been implicated as important pathogenic factors due to their promotion of oxidative stress. It was recently reported that the plasma and tissue protein alpha1-microglobulin is involved in heme metabolism. The protein binds heme, and a carboxy-terminally processed form, truncated alpha1-microglobulin, also degrades heme. Here, we show the presence of micromolar levels of heme and free iron in chronic leg ulcer fluids. Micromolar amounts of alpha1-microglobulin was also present in the ulcer fluids and bound to added radiolabeled heme. Truncated alpha1-microglobulin was found in the ulcer fluids and exogenously added alpha1-microglobulin was processed into the truncated alpha1-microglobulin form. Histochemical analysis of chronic wound tissue showed the presence of iron deposits, heme/porphyrins in infiltrating cells basement membranes and fibrin cuffs around vessels, and alpha1-microglobulin ubiquitously distributed but especially abundant in basement membranes around vessels and at fibrin cuffs. Our results suggest that alpha1-microglobulin constitutes a previously unknown defense mechanism against high heme and iron levels during skin wound healing. Excessive heme and iron, which are not buffered by alpha1-microglobulin, may underlie the chronic inflammation in chronic ulcers.
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| 2. |
- Andersson, Tilde, et al.
(författare)
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Common skin bacteria protect their host from oxidative stress through secreted antioxidant RoxP
- 2019
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Cutibacterium acnes is an abundant skin commensal with several proposed mutualistic functions. A protein with strong antioxidant activity was recently identified from the C. acnes secretome. This protein, termed RoxP, facilitated aerobic bacterial growth in vitro and ex vivo. As reducing events naturally occurred outside of the bacterial cell, it was further hypothesized that RoxP could also serve to modulate redox status of human skin. The biological function of RoxP was here assessed in vitro and in vivo, through oxidatively stressed cell cultures and through protein quantification from skin affected by oxidative disease (actinic keratosis and basal cell carcinoma), respectively. 16S rDNA amplicon deep sequencing and single locus sequence typing was used to correlate bacterial prevalence to cutaneous RoxP abundances. We show that RoxP positively influence the viability of monocytes and keratinocytes exposed to oxidative stress, and that a congruent concentration decline of RoxP can be observed in skin affected by oxidative disease. Basal cell carcinoma was moreover associated with microbial dysbiosis, characterized by reduced C. acnes prevalence. C. acnes's secretion of RoxP, an exogenous but naturally occurring antioxidant on human skin, is likely to positively influence the human host. Results furthermore attest to its prospective usability as a biopharmaceutical.
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| 3. |
- Bernhardsson, Susanne, 1958, et al.
(författare)
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Implementation of physical activity on prescription for children with obesity in paediatric health care (IMPA): protocol for a feasibility and evaluation study using quantitative and qualitative methods
- 2022
-
Ingår i: Pilot and Feasibility Studies. - : Springer Science and Business Media LLC. - 2055-5784. ; 8:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: Physical inactivity is a main cause of childhood obesity which tracks into adulthood obesity, making it important to address early in life. Physical activity on prescription (PAP) is an evidence-based intervention that has shown good effect on physical activity levels in adults, but has not been evaluated in children with obesity. This project aims to evaluate the prerequisites, determinants, and feasibility of implementing PAP adapted to children with obesity and to explore children's, parents', and healthcare providers' experiences of PAP. Methods: In the first phase of the project, healthcare providers and managers from 26 paediatric clinics in Region Vastra Gotaland, Sweden, will be invited to participate in a web-based survey and a subset of this sample for a focus group study. Findings from these two data collections will form the basis for adaptation of PAP to the target group and context. In a second phase, this adapted PAP intervention will be evaluated in a clinical study in a sample of approximately 60 children with obesity (ISO-BMI > 30) between 6 and 12 years of age and one of their parents/legal guardians. Implementation process and clinical outcomes will be assessed pre- and post-intervention and at 8 and 12 months' follow-up. Implementation outcomes are the four core constructs of the Normalization Process Theory; coherence, cognitive participation, collective action, and reflexive monitoring; and appropriateness, acceptability, and feasibility of the PAP intervention. Additional implementation process outcomes are recruitment and attrition rates, intervention fidelity, dose, and adherence. Clinical outcomes are physical activity pattern, BMI, metabolic risk factors, health-related quality of life, sleep, and self-efficacy and motivation for physical activity. Lastly, we will explore the perspectives of children and parents in semi-structured interviews. Design and analysis of the included studies are guided by the Normalization Process Theory. Discussion: This project will provide new knowledge regarding the feasibility of PAP for children with obesity and about whether and how an evidence-based intervention can be fitted and adapted to new contexts and populations. The results may inform a larger scale trial and future implementation and may enhance the role of PAP in the management of obesity in paediatric health care in Sweden.
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| 4. |
- Boman, Charlotte, et al.
(författare)
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Physical activity on prescription for children with obesity: a focus group study exploring experiences in paediatric healthcare
- 2024
-
Ingår i: Frontiers in Health Services. - 2813-0146. ; 4
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Insufficient physical activity is a growing public health concern and is closely linked to obesity in both adults and children. Swedish physical activity on prescription (PAP) is effective in increasing physical activity levels in adults, but knowledge about how PAP is used in paediatric healthcare is lacking. Therefore, this study aimed to explore experiences of working with PAP for children with obesity amongst paediatric staff and managers. Methods: Seven focus group discussions with 26 participants from paediatric outpatient clinics in western Sweden were conducted. Data were analysed both inductively and deductively, framed by the Normalization Process Theory's four core constructs: coherence, cognitive participation, collective action, and reflexive monitoring. Results: The PAP work for children with obesity was experienced to be about helping children to become physically active, and less about losing weight. Identified barriers for using PAP were the non-uniform nature of the work and a perceived lack of guidelines. Collaboration with physiotherapists and physical activity organisers outside the organisation was identified as an important facilitator. An important contextual factor for implementing PAP is the collaboration between paediatric clinics and physical activity organisers. In the transition between these stakeholders, maintaining a family-centred approach when working with PAP was experienced as challenging. Conclusions: PAP is a well-known intervention that is inconsistently used for children with obesity. The intervention should include a family-centred approach for this patient group. It also needs to align better with existing collaborations with other healthcare units as well as with new forms of collaboration with physical activity organisers in the community.
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| 5. |
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| 6. |
- Boman, Charlotte, et al.
(författare)
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Prerequisites for implementing physical activity on prescription for children with obesity in paediatric health care: A cross-sectional survey.
- 2023
-
Ingår i: Frontiers in Health Services. - : Frontiers Media SA. - 2813-0146. ; 2
-
Tidskriftsartikel (refereegranskat)abstract
- Physical inactivity is a main driver of childhood obesity that tracks into adulthood, making it crucial to address early in life. Swedish physical activity on prescription (PAP) is an effective intervention for increasing physical activity levels in adults and is being implemented in primary care in Sweden. Before implementing PAP for children, both intervention effectiveness and implementation prerequisites need to be examined. Framed by the Normalization Process Theory (NPT) domains, this study aimed to investigate perceptions of PAP amongst paediatric staff and managers working with children with obesity, as well as acceptability, appropriateness, feasibility, and barriers and facilitators for implementing PAP in paediatric health care.Staff and managers in 28 paediatric outpatient clinics in western Sweden were surveyed using validated implementation instruments and open-ended questions. Data were analysed using Mann-Whitney U tests and Kruskal-Wallis tests. Qualitative data were categorised into NPT domains.The survey response rate was 54% (125/229). Most respondents (82%) reported PAP to be familiar and many (56%) perceived it as a normal part of work; nurses and physiotherapists to a greater extent (p<0.001). This was anticipated to increase in the future (82%), especially amongst those with the longest work experience (p=0.012). Respondents reported seeing the potential value in their work with PAP (77%), being open to working in new ways to use PAP (94%), and having confidence in their colleagues' ability to use PAP (77%). Barriers and facilitators were found in all the NPT domains, mainly collective action and reflexive monitoring, where, for example, inadequacies of education, resources, and research on PAP for children were reported as barriers. Most respondents agreed that PAP was acceptable, appropriate, and feasible (71% to 88%).PAP is familiar and perceived as an acceptable, appropriate, and feasible intervention, and by many viewed as a normal part of clinical routines in paediatric outpatient clinics in western Sweden, especially by physiotherapists and nurses. Barriers and faciliators are mainly related to collective action and reflexive monitoring. The wide acceptance demonstrates receptiveness to PAP as an intervention to promote an active lifestyle for children with obesity.
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| 7. |
- Bäcklund, Katarina, 1982-, et al.
(författare)
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Building Occupants, Their Behavior and the Resulting Impact on Energy Use in Campus Buildings : A Literature Review with Focus on Smart Building Systems
- 2023
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Ingår i: Energies. - : MDPI AG. - 1996-1073. ; 16:17, s. 6104-6104
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Tidskriftsartikel (refereegranskat)abstract
- In the light of global climate change and the current energy crisis, it is crucial to target sustainable energy use in all sectors. Buildings still remain one of the most energy-demanding sectors. Campus buildings and higher educational buildings are important to target due to their high and increasing energy demand. This building segment also represents a research gap, as mostly office or domestic buildings have been studied previously. In the quest for thermal comfort, a key stakeholder in building energy demand is the building occupant. It is therefore crucial to promote energy-aware behaviors. The building systems are another key factor to consider. As conventional building systems are replaced with smart building systems, the entire scenario is redrawn for how building occupants interact with the building and its systems. This study argues that behavior is evolving with the smartness of building systems. By means of a semi-systematic literature review, this study presents key findings from peer-reviewed research that deal with building occupant behavior, building systems and energy use in campus buildings. The literature review was an iterative process based on six predefined research questions. Two key results are presented: a graph of reported energy-saving potentials and a conceptual framework to evaluate building occupants impact on building energy use. Furthermore, based on the identified research gaps in the selected literature, areas for future research are proposed.
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| 8. |
- Bäcklund, Katarina, 1982-
(författare)
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Human-Centric Operations of Smarter Higher Educational Buildings in Sweden
- 2024
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This PhD thesis is based on four articles, each focused on distinct research areas: building occupants, smarter higher educational buildings, smart building systems and the digital transformation of real estate organizations. Rather than concentrating on one specific research area, the objective was to provide a comprehensive view of the broader system and delve into these four key research areas. Thus, they are all interdependent and require equal attention to facilitate the digital transformation within the commercial real estate industry. This transformation aims to create a more climate neutral built environment while ensuring good quality indoor comfort. The findings reveal a paradox within smart building systems, where building occupants are often treated as passive agents and interactions are intentionally reduced. However, it can be argued that some interactions will be beneficial, as building occupants considered as active agents could contribute to a more sustainable building operation. This study also concludes that current smart buildings are not yet smart enough and that all data is not smart data. Furthermore, the investigation into what constitutes a smart building has led to the identification of key features of smarter higher educational buildings. Finally, the concluding part of this study delved into the organizational challenges associated with digital transformation, proposing potential solutions to enhance innovation processes. Based on these findings, a framework for human-centric digital transformation was proposed.
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| 9. |
- Bäcklund, Katarina, 1982-, et al.
(författare)
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In Search for Untapped Energy-Saving Potential in Green and Smart Higher Educational Buildings—An Empirical Case Study Involving the Building Occupants
- 2023
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Ingår i: Buildings. - : MDPI AG. - 2075-5309. ; 13:12
-
Tidskriftsartikel (refereegranskat)abstract
- Energy-intense activities and the unpredictable and complex behavior of building occupants lead to an increase in building energy demand. It is, therefore, crucial to study underlying factors for building energy demand related to the users. Higher educational buildings are relevant to study for several reasons: they host the future workforce and citizens, they are predicted to increase in numbers, and they represent a building type less studied. Furthermore, green-rated buildings equipped with smart building systems also represent a research gap that is relevant to address since such a building design involves IoT-functionalities and digital features for the building occupants to interact with. There is also a conceivable risk that if the users know that the building is green-rated and technologically advanced, this may alter their perception of the building operation and thus their behavior. To study the relationship between building occupants and such green and smart educational structure, a survey was conducted in a Swedish higher educational building; as a result, 300 responses were collected and analyzed. The responses revealed that the building occupants act with energy awareness, and they are conscious about energy-saving behaviors. One building feature in particular was studied: the Digital Room Panels (DRPs). The DRP allows the building occupants to modify the indoor temperature and is, therefore, essential for thermal comfort. One key finding from the survey revealed that 70% of the building occupants did not know how the DRPs operate. This study argues that this result can be explained with a lack of communication and user friendliness. Inadequate interactions with building systems could also result in opportunities for energy saving might not be realized. The findings of this case study led to valuable recommendations and suggestions for future research endeavors.
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| 10. |
- Bäcklund, Katarina, 1982-, et al.
(författare)
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Showcasing a Digital Twin for Higher Educational Buildings : Developing the Concept Towards Human Centricity
- 2024
-
Ingår i: Frontiers in Built Environment. - : Frontiers Media SA. - 2297-3362. ; 10
-
Tidskriftsartikel (refereegranskat)abstract
- Digital twin technology is an emerging technology within the built environment. There are yet many unexplored opportunities to utilize digital twins for facilitating the transformation towards a climate neutral building stock while also meeting the expectations from the building occupants. This article presents a case study of a digital twin, developed for an existing commercial building stock of campus areas in Sweden. The overarching purpose of the digital twin is to support both building occupants and building operators. This twofold human-centric approach represents a novel approach for building digital twins. The digital twin is based on 3D scanning and together with geospatial data, a real-like navigational indoor environment is created. Three innovative features are presented; the building analysis module, the digital twin mobile application and the building operations module. The results show that the digital twin improves the building occupant’s experience by supporting navigation and providing access to room booking system via this dedicated interface. Building management is also benefited by the digital twin through easier access to building data aggregated into one platform and a state-of-the-art analysis tool for optimizing the use of indoor space. The digital twin holds future potential to achieve operational excellence by incorporating feedback mechanisms and utilizing Artificial Intelligence to enable intelligent fault detection and prevention. Keywords:
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| 11. |
- Bäcklund, Katarina, 1982-, et al.
(författare)
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Showcasing the First Steps Towards a Digital Twin for Campus Environments
- 2022
-
Ingår i: 2022 BuildSim Nordic. - : EDP Sciences.
-
Konferensbidrag (refereegranskat)abstract
- This paper presents a path towards the implementation of a Digital Twin for campus environments. The main purpose of the Digital Twin is to accomplish an advanced analytical tool, which supports building owners, building operators and building users to reach an improved performance of the building. Digital Twins is new to the building and the real estate industry, hence research within this field is scarce. This paper contributes to the research by providing a methodology to implement a Digital Twin of an existing building stock of campus areas in Sweden. The main results obtained so far are presented. They indicate that the potential of a Digital Twin expands beyond the aspects of a navigational digital 3D model, including a state-of-the-art app that is developed from the Digital Twin platform.
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| 12. |
- Chaudhari, Aditi, et al.
(författare)
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p110alpha hot spot mutations E545K and H1047R exert metabolic reprogramming independently of p110alpha kinase activity : Kinase-independent signaling of p110 alpha mutants
- 2015
-
Ingår i: Molecular and Cellular Biology. - 0270-7306 .- 1098-5549. ; 35:19, s. 3258-3273
-
Tidskriftsartikel (refereegranskat)abstract
- The phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) catalytic subunit p110α is the most frequently mutated kinase in human cancer, and the hot spot mutations E542K, E545K, and H1047R are the most common mutations in p110α. Very little is known about the metabolic consequences of the hot spot mutations of p110α in vivo. In this study, we used adenoviral gene transfer in mice to investigate the effects of the E545K and H1047R mutations on hepatic and whole-body glucose metabolism. We show that hepatic expression of these hot spot mutations results in rapid hepatic steatosis, paradoxically accompanied by increased glucose tolerance, and marked glycogen accumulation. In contrast, wild-type p110α expression does not lead to hepatic accumulation of lipids or glycogen despite similar degrees of upregulated glycolysis and expression of lipogenic genes. The reprogrammed metabolism of the E545K and H1047R p110α mutants was surprisingly not dependent on altered p110α lipid kinase activity.
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| 13. |
- Chaudhari, Aditi, et al.
(författare)
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p110α hot spot mutations E545K and H1047R exert metabolic reprogramming independently of p110α kinase activity
- 2015
-
Ingår i: Molecular and Cellular Biology. - : American Society for Microbiology. - 0270-7306 .- 1098-5549. ; 35:19, s. 3258-3273
-
Tidskriftsartikel (refereegranskat)abstract
- The phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) catalytic subunit p110α is the most frequently mutated kinase in human cancer, and the hot spot mutations E542K, E545K, and H1047R are the most common mutations in p110α. Very little is known about the metabolic consequences of the hot spot mutations of p110α in vivo. In this study, we used adenoviral gene transfer in mice to investigate the effects of the E545K and H1047R mutations on hepatic and whole-body glucose metabolism. We show that hepatic expression of these hot spot mutations results in rapid hepatic steatosis, paradoxically accompanied by increased glucose tolerance, and marked glycogen accumulation. In contrast, wild-type p110α expression does not lead to hepatic accumulation of lipids or glycogen despite similar degrees of upregulated glycolysis and expression of lipogenic genes. The reprogrammed metabolism of the E545K and H1047R p110α mutants was surprisingly not dependent on altered p110α lipid kinase activity.
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| 14. |
- Gedda, Lars, et al.
(författare)
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Experimental radionuclide therapy of HER2-expressing xenografts using two-step targeting Nuclisome-particles
- 2012
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Ingår i: Journal of Nuclear Medicine. - : Society of Nuclear Medicine. - 0161-5505 .- 1535-5667 .- 2159-662X. ; 53:3, s. 480-487
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Tidskriftsartikel (refereegranskat)abstract
- The therapeutic potential of Auger-electron emitting radionuclides is strongly dependent on their close vicinity to DNA, since the energy deposition is mainly localized within a few cubic nanometers around the site of decay. Thus, apart from specificity, successful tumor therapy relies on a nuclear delivery strategy. We recently presented a two-step targeting strategy to transport Auger-electron-emitting radionuclides into the cell nucleus by means of nuclide-filled liposomes (Nuclisome particles), that is, polyethylene glycol-stabilized, tumor-cell-targeting liposomes loaded with (125)I-labeled anthracyclines. In the present study, the survival of mice intraperitoneally inoculated with human HER2-expressing SKOV-3 tumor cells and treated with HER2-targeting Nuclisome particles was studied.METHODS:BALB/c nu/nu mice were inoculated with 10(7) SKOV-3 cells intraperitoneally and thereafter directly injected with Nuclisome particles with increasing specific radioactivity. Groups of 10-12 mice were treated with 0.01 MBq/mouse up to 2 MBq/mouse, and survival was monitored and compared with that in control groups (n = 33). Organs were analyzed for HER2 expression and radiotoxic effects histologically. Absorbed doses were estimated using dose factors from the online Radiation Dose Assessment Resource model.RESULTS:The results showed a clear correlation between administered radioactive dose and survival. No such dose-dependent survival was observed for mice treated with Nuclisome particles lacking HER2-targeting ability. With HER2-targeting Nuclisome particles, a significant increase in survival, compared with that of untreated control mice, could already be seen at an administered activity of 0.1 MBq/mouse (P = 0.0301). At the highest activity administered, 2 MBq/mouse (P < 0.0001), 70% of the mice survived the study and most were tumor-free. Neither macroscopic nor microscopic radiotoxic side effects were observed. Dosimetric calculations, assuming nonreceptor targeting, revealed that the radioactive doses to normal tissues were low.CONCLUSION:Taken together the results show that with successful targeting to the tumor-cell nucleus it is possible to obtain a therapeutic effect from Auger-electron-emitting radionuclides administered at radioactive doses low enough to spare normal tissue from radiotoxic side effects.
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| 15. |
- Gram, Magnus, et al.
(författare)
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Up-Regulation of A1M/α(1)-Microglobulin in Skin by Heme and Reactive Oxygen Species Gives Protection from Oxidative Damage.
- 2011
-
Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 6:11
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Tidskriftsartikel (refereegranskat)abstract
- During bleeding the skin is subjected to oxidative insults from free heme and radicals, generated from extracellular hemoglobin. The lipocalin α(1)-microglobulin (A1M) was recently shown to have reductase properties, reducing heme-proteins and other substrates, and to scavenge heme and radicals. We investigated the expression and localization of A1M in skin and the possible role of A1M in the protection of skin tissue from damage induced by heme and reactive oxygen species. Skin explants, keratinocyte cultures and purified collagen I were exposed to heme, reactive oxygen species, and/or A1M and investigated by biochemical methods and electron microscopy. The results demonstrate that A1M is localized ubiquitously in the dermal and epidermal layers, and that the A1M-gene is expressed in keratinocytes and up-regulated after exposure to heme and reactive oxygen species. A1M inhibited the heme- and reactive oxygen species-induced ultrastructural damage, up-regulation of antioxidation and cell cycle regulatory genes, and protein carbonyl formation in skin and keratinocytes. Finally, A1M bound to purified collagen I (K(d) = 0.96×10(-6) M) and could inhibit and repair the destruction of collagen fibrils by heme and reactive oxygen species. The results suggest that A1M may have a physiological role in protection of skin cells and matrix against oxidative damage following bleeding.
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| 16. |
- Götherström, Cecilia, et al.
(författare)
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Fetal and adult multipotent mesenchymal stromal cells are killed by different pathways
- 2011
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Ingår i: Cytotherapy. - : Elsevier BV. - 1465-3249 .- 1477-2566. ; 13:3, s. 269-278
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Tidskriftsartikel (refereegranskat)abstract
- Background aims. Multipotent mesenchymal stromal cells, also known as mesenchymal stem cells (MSC), can be isolated from adult and fetal tissues. Recently, there has been considerable interest in MSC because they have features favorable for transplantation, namely their multipotency and non-immunogenic properties. Methods. We analyzed how human MSC derived from first-trimester fetal liver and adult bone marrow interact with naive and activated innate natural killer (NK) cells. NK cell function was studied by measuring killing of MSC, as well as degranulation (CD107a) induced by MSC. To assess the importance of NK cell killing, expression of surface epitopes was analyzed by flow cytometry on MSC before and after stimulation with interferon (IFN)gamma gamma. Results. Fetal and adult MSC express several ligands to activating NK cell receptors as well as low levels of HLA class I, with large inter-individual variation. Naive peripheral blood NK cells did not lyse fetal or adult MSC, whereas interleukin (IL)2 activated allogeneic as well as autologous NK cells did. Pre-incubation of MSC with IFN-gamma gamma increased their levels of HLA class I, protecting them from NK cell recognition. Fetal and adult MSC were preferably killed via the tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and Fas ligand (FasL) pathways, respectively. Blocking NKG2D reduced NK cell degranulation in both fetal and adult MSC. Conclusions. Fetal and adult MSC differ in their interactions with NK cells. Both fetal and adult MSC are susceptible to lysis by activated NK cells, which may have implications for the use of MSC in cell therapy.
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| 17. |
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| 18. |
- Johannesson, Tomas, 1975-
(musiker, creator_code:sds_t, creator_code:cre_t)
-
Norrbotten blues : CD-produktion av Ronny Eriksson och Ramblin' Minds
- 2019
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Konstnärligt arbeteabstract
- CD-inspelning: Norrbotten blues - Ronny Eriksson & Ramblin' MindsLåtlista1. Norrbotten Blues2. Gråtvals3. På kanten av en grop4. Flyktingarna5. Då tänker jag på Syrien6. Låt mig gå, jag har inte tid att stanna7. Tröstvals8. Blues för ingen alls9. Brorsan10. Den officiella versionen11. Blues under fullmånenMusikerRonny Eriksson - sångMikael Bäckman - munspelLeo Holmberg - gitarr/körAnders Wedin - gitarrAnna Wedin - bas/körKatarina Pettersson - trummorMathias Lundqvist - piano/orgelTomas Johannesson - barytonsaxStefan Tjerngren - tenorsaxTomas Johannesson - Ljudtekniker (inspelning, redigering och mix)Stockholm Mastering - masteringRootsy Music - bolagInspelat på musikhögskolan i Piteå 7-9 september 2018.Publicerad 9 mars 2019.
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| 19. |
- Khmaladze, Ia, et al.
(författare)
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Mannan induces ROS-regulated, IL-17A-dependent psoriasis arthritis-like disease in mice
- 2014
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - Washington, DC : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 111:35, s. E3669-E3678
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Tidskriftsartikel (refereegranskat)abstract
- Psoriasis (Ps) and psoriasis arthritis (PsA) are poorly understood common diseases, induced by unknown environmental factors, affecting skin and articular joints. A single i.p. exposure to mannan from Saccharomyces cerevisiae induced an acute inflammation in inbred mouse strains resembling human Ps and PsA-like disease, whereas multiple injections induced a relapsing disease. Exacerbation of disease severity was observed in mice deficient for generation of reactive oxygen species (ROS). Interestingly, restoration of ROS production, specifically in macrophages, ameliorated both skin and joint disease. Neutralization of IL-17A, mainly produced by gammadelta T cells, completely blocked disease symptoms. Furthermore, mice depleted of granulocytes were resistant to disease development. In contrast, certain acute inflammatory mediators (C5, Fcgamma receptor III, mast cells, and histamine) and adaptive immune players (alphabeta T and B cells) were redundant in disease induction. Hence, we propose that mannan-induced activation of macrophages leads to TNF-alpha secretion and stimulation of local gammadelta T cells secreting IL-17A. The combined action of activated macrophages and IL-17A produced in situ drives neutrophil infiltration in the epidermis and dermis of the skin, leading to disease manifestations. Thus, our finding suggests a new mechanism triggered by exposure to exogenous microbial components, such as mannan, that can induce and exacerbate Ps and PsA.
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| 20. |
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| 21. |
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| 22. |
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| 23. |
- Lundqvist, Anette, 1963-
(författare)
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Nutritional aspects of behaviour and biology during pregnancy and postpartum
- 2016
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- BackgroundA well-balanced nutritious diet is important for the pregnant woman and the growing fetus, as well as for their future health. Poor nutrition results from both over-consumption of energy-rich foods which can lead to a higher weight gain than is healthy and under-nutrition of essential nutrients. Food intake is regulated in complex biological systems by many factors, where steroid hormone is one factor involved.The overall aim of this thesis is to describe dietary intake, vitamin D levels, dietary information and dietary changes, and to study the relation between allopregnanolone and weight gain during pregnancy and postpartum.Methods Study I was a qualitative study with focus group interviews with 23 pregnant women. The text was analysed with content analysis. Study II was a quantitative cross-sectional study conducted in early pregnancy (n=209) with a reference group (n=206). Self-reported dietary data from a questionnaire was analysed using descriptive comparative statistics and a cluster analysis model (Partial Least Squares modelling). Study III had a quantitative longitudinal design. Vitamin D concentrations were analysed in 184 women, collected on five occasions during pregnancy and postpartum. Descriptive comparative statistics and a linear mixed model were used. Study IV was a quantitative longitudinal study with 60 women. Concentrations of allopregnanolone were analysed in gestational week 12 and 35. Descriptive and comparative statistics as well as Spearman’s correlation (rho) were used to describe the relationship between weight gain and allopregnanolone concentrations. Results The focus group interviews showed that women wanted to know more about different foods to reduce any risk for their child but the information about foods was partly up to themselves to find out. They expressedfeelingsof insecurityand guiltif they accidentallyate something“forbidden”. The recommendationswere followedas best as possiblealong withcommon sense todeal with dietchanges. The main themes were “Finding out by oneself”, “Getting professional advice when health problems occur”, “Being uncertain” and “Being responsible with a pinch of salt”. Some differences in the dietary patterns were found among the pregnant women compared to references, with less, vegetables (47 g/day), potatoes/rice/pasta (31 g/day), meat/fish (24 g/day) and intake of alcohol and tobacco/snuff but a higher intake of supplements. Bothpregnant women and referenceshad intakes offolatethrough diet45% (pregnant) and 22% (references) lower than current recommendations(500vs400g/day). Vitamin Dintake was34% lower than the recommendationsof 10mg/day. At least a third of the participants had insufficient plasma levels below 50 nmol/L of vitamin D. Season was a strong factor influencing the longitudinal pattern. Gestational week, season, total energy intake, dietary intake of vitamin D, and multivitamin supplementation over the previous 14 days were factors related to vitamin D levels. A correlation betweenallopregnanoloneconcentrations ingestationalweek 35and weight gainin weeks12–35was seen (p = 0.016). Therewas alsoa correlation betweenthe increase inallopregnanolone(weeks12–35) andweight gain(see above) (p = 0.028). ConclusionsDietary recommendations were described as contradictory and confusing and the dietary advice felt inadequate. The women faced their diet changes and sought information on their own but would have wished for more extensive advice from the midwife. The intake of vitamins essential for pregnancy was lower than recommended, which is also confirmed by low plasma levels of vitamin D in at least one third of the pregnant women. Vitamin D levels peaked in late pregnancy. Aside from gestational week and season which were related to plasma levels, intake from foods and supplements also affected the levels. Reasons for weight gain are complex and depend on many factors. Allopregnanolone is a factor that was seen to relate to the weight gain of the studied pregnant women.
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| 24. |
- Lundqvist, Anette, et al.
(författare)
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Reported dietary intake in early pregnant compared to non-pregnant women : a cross-sectional study
- 2014
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Ingår i: BMC Pregnancy and Childbirth. - : BioMed Central. - 1471-2393 .- 1471-2393. ; 14:373
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Tidskriftsartikel (refereegranskat)abstract
- Background: A woman's nutritional status before conception and during pregnancy is important for maternal health and the health of the foetus. The aim of the study was to compare diet intake in early pregnant women with non-pregnant women. Methods: Between September 2006 and March 2009, 226 women in early pregnancy were consecutively recruited at five antenatal clinics in Northern Sweden. Referent women (n = 211) were randomly selected from a current health screening project running in the same region (the Vasterbotten Intervention Program; VIP). We collected diet data with a self-reported validated food frequency questionnaire with 66 food items/food aggregates, and information on portion size, alcohol consumption, and supplement intake. Data were analysed using descriptive, comparative statistics and multivariate partial least square modelling. Results: Intake of folate and vitamin D from foods was generally low for both groups. Intake of folate and vitamin D supplements was generally high in the pregnant group and led to significantly higher total estimated intake of vitamin D and folate in the pregnant group. Iron intake from foods tended to be lower in pregnant women although iron supplement intake evened out the difference with respect to iron intake from foods only. Energy intake was slightly lower in pregnant women but not significant, a reflection of that they reported consuming significantly less of potatoes/rice/pasta, meat/fish, and vegetables (grams/day) than the women in the referent group. Conclusions: In the present study, women in early pregnancy reported less intake of vegetables, potatoes, meat, and alcohol than non-pregnant women. As they also had a low intake (below the Nordic Nutritional Recommendations) of folate, vitamin D, and iron from foods, some of these women and their unborn children are possibly at risk for adverse effects on the pregnancy and birth outcome.
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| 25. |
- Lundqvist, Adam, et al.
(författare)
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Validation of the IHE Cohort Model of Type 2 Diabetes and the Impact of Choice of Macrovascular Risk Equations.
- 2014
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 9:10
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Tidskriftsartikel (refereegranskat)abstract
- Health-economic models of diabetes are complex since the disease is chronic, progressive and there are many diabetic complications. External validation of these models helps building trust and satisfies demands from decision makers. We evaluated the external validity of the IHE Cohort Model of Type 2 Diabetes; the impact of using alternative macrovascular risk equations; and compared the results to those from microsimulation models.
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| 26. |
- Lundqvist, Katarina, et al.
(författare)
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Cyclin A expression in chronic leg ulcers
- 2006
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Ingår i: Acta Dermato-Venereologica. - : Medical Journals Sweden AB. - 1651-2057 .- 0001-5555. ; 86:1, s. 61-62
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 27. |
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| 28. |
- Lundqvist, Katarina, et al.
(författare)
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Immunohistochemical studies on proteoglycan expression in normal skin and chronic ulcers
- 2001
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Ingår i: British Journal of Dermatology. - : Oxford University Press (OUP). - 1365-2133 .- 0007-0963. ; 144:2, s. 254-259
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Proteoglycans (PGs) represent a large family of complex molecules. They are found either as integral membrane components or constituents of the extracellular matrix. Their protein backbones are linked to different glycosaminoglycans, such as dermatan-, chondroitin-, keratan- or heparan sulphate. The molecules have specific functions during developmental processes as well as in diseases, such as cancer and inflammation. OBJECTIVES: The expression patterns of various cell-associated heparan and chondroitin/dermatan-sulphate PGs in human skin and chronic venous ulcers were investigated. METHODS: Tissue sections from 11 patients with chronic venous ulcers were used in this study. Monoclonal antibodies were used for detection of the proteoglycans syndecan-1, -2 and -4, glypican, CD44 and perlecan. RESULTS: The different PGs exhibited individual staining patterns. Syndecan-1 and -4 and glypican expression in chronic ulcers differed from the staining in normal skin. Whereas the expression of syndecan-4 and glypican in intact skin was mostly in the pericellular regions of keratinocytes, the epidermal cells from the wound edge contained mostly intracellular PGs. In the wound edge, syndecan-4 was predominantly expressed by epidermal basal layer cells. Syndecan-1 was less expressed at the epidermal wound margins. PGs bind growth factors, regulate proteolytic activity and act as matrix receptors. CONCLUSIONS: The altered expression patterns of glypican and syndecan-1 and -4 in chronic ulcers reflect their possible roles during inflammation and cell proliferation. Hence, analysis of PG expression should be of interest in future studies on normal as well as defective wound healing.
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| 29. |
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| 30. |
- Lundqvist, Katarina
(författare)
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Molecular and morphological studies on non-healing venous leg ulcers.
- 2006
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Chronic skin ulcers are a major health problem and an increasing burden on health care providers. These ulcers have various underlying causes, such as venous or arterial insufficiency, diabetes, or vasculitis. Non-healing venous leg ulcers represent a condition characterized by excessive inflammation and presence of bacteria. The overall aim of this thesis has been to explore the pathogenesis of these ulcers, with particular focus on bacterial influence, capillary leakage, neutrophil efflux, and oxidative stress as well as cell growth. The results showed that cyclin A, a proliferation marker, was upregulated in the margins of chronic venous ulcers, at levels similar to those detected in acute wounds. Furthermore, several cell-associated proteoglycans were detected in the wound margins of venous ulcers, suggesting that epidermal cells in these ulcers are able to proliferate as well as express molecules important for cell homeostasis and growth. The venous ulcers contained significantly increased levels of chemotactic ?-defensins, as well as heparin binding protein (HBP), a factor known to induce capillary leakage. Secreted products of Pseudomonas aeruginosa, a bacterium often present in chronic ulcers, were found to induce release of HBP from human neutrophils. Chronic venous leg ulcers contained high levels of heme and porphyrin. Furthermore, a novel heme-scavenger, ?1-microglobulin, was identified in these ulcers. Taken together, the data suggests a possible link between bacterial presence, neutrophil activation and HBP-release in venous ulcers, which will help to explain the clinical observation of classic inflammatory signs such as high exudation, swelling, and erythema often accompanied by P. aeruginosa infected wounds. Thus, high HBP and defensin levels, promoting capillary leakage and chemotaxis, respectively, in concert with heme-mediated oxidative stress, may constitute a "vicious loop", which underlies the high inflammation noted in non-healing venous ulcers. These findings provide novel diagnostic markers and reinforce the view that future therapeutic approaches directed at venous ulcers should target inflammatory mechanisms and excessive bacterial influence.
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| 31. |
- Lundqvist, Lars-Olov, 1958-, et al.
(författare)
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Influence of mental health service provision on the perceived quality of life among psychiatric outpatients : associations and mediating factors
- 2024
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Ingår i: Frontiers in Psychiatry. - : Frontiers Media S.A.. - 1664-0640. ; 14
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Tidskriftsartikel (refereegranskat)abstract
- Objective To investigate the relationship between perceived mental health service provision and quality of life (QoL) as perceived by patients in psychiatric outpatient care.Methods A total of 373 adult patients registered at 15 psychiatric outpatient clinics in three regions in central and southern Sweden were included in the study. Survey data were collected using a questionnaire on mental health service provision, symptom severity, recovery, clinical diagnosis, sociodemographics (serving as independent variables) and QoL (serving as the dependent variable). Three aspects of mental health service provision were used: patients' perceived quality of care, perceived staff-patient interaction, and patient reported psychiatric treatments. Structural equation modelling was used to model the relationship among the variables.Results Variables in mental health service provision showed few direct associations with patients' perceived QoL. Instead, the associations of mental health service provision on QoL were mainly mediated through symptom severity and recovery. These relationships were retained after adjusting for sociodemographic variables and clinical diagnoses. The final model achieved excellent goodness of fit (chi 2 = 49.502, p = 0.230, RMSEA = 0.020, CFI = 0.997 and a SRMR = 0.024).Conclusion This study shows that mental health service provision is associated with patients' perceived QoL; however, this association is mostly indirect and mediated by reduced symptom severity and increased recovery. This finding can help inform the design of future interventions to enhance service provision to improve patients' QoL.
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| 32. |
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| 33. |
- Nätterdahl, Carolina, et al.
(författare)
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Risk Factors for Complicated Mohs Surgery in the South Sweden Mohs Cohort
- 2022
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Ingår i: Journal of the European Academy of Dermatology and Venereology. - : Wiley. - 1468-3083 .- 0926-9959. ; 36:7, s. 1113-1117
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Tidskriftsartikel (refereegranskat)abstract
- Background: Mohs micrographic surgery (MMS) is a precise, tissue-sparing surgical technique that offers superior cure rates compared to traditional surgical excision. However, the degree of difficulty of MMS depends on many variables, and consequently, the number of surgical stages required for each case is quite unpredictable.Objectives: To identify risk factors for complicated MMS, defined as MMS requiring ≥3 stages.Methods: In a cohort study design, data were prospectively collected from 612 patients that underwent MMS for basal cell carcinoma (BCC) at the Department of Dermatology, Skåne University Hospital, Lund, between 2009 and 2020. Univariate and multivariate logistic regression were used to estimate the risk of MMS requiring ≥3 stages. Due to the risk of multicollinearity between recurrent or incompletely excised BCC and previous treatments, a partially and a fully adjusted multivariate logistic regression model were constructed.Results: In fully adjusted multivariate analyses, age (odds ratio (OR) 1.02; confidence interval (CI) 95% 1.00-1.04), previous cryotherapy (OR 2.3; CI 95% 1.1-4.8), and >1 previous surgery (OR 3.4; CI 95% 1.5-7.7) were significantly associated with risk of complicated MMS. Recurrent BCC was associated with the risk of complicated MMS in partially adjusted multivariate analyses, but not in the fully adjusted analyses. In this highly selected cohort, histopathological subtype, and tumour localization were not associated with the risk of complicated MMS.Conclusions: Older age and tumours previously treated with cryotherapy or multiple prior surgeries increased the risk of MMS requiring ≥3 stages. Whether recurrent BCC is an independent risk factor for complicated MMS needs further evaluation. Knowledge of these risk factors may ameliorate the planning of Mohs surgeries.
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| 34. |
- Ohm, Lotta, et al.
(författare)
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Real-world cost-effectiveness in chronic myeloid leukemia: the price of success during four decades of development from non-targeted treatment to imatinib
- 2015
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Ingår i: Leukemia & Lymphoma. - : Informa UK Limited. - 1042-8194 .- 1029-2403. ; 56:5, s. 1385-1391
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Tidskriftsartikel (refereegranskat)abstract
- Imatinib has revolutionized the treatment of chronic myeloid leukemia (CML). We evaluated clinical outcome and cost-effectiveness, using Swedish registry data based on patients with CML diagnosed 1973-2008. Outcome from three time periods (I: 1973-1979; II: 1991-1997; III: 2002-2008) associated with symptomatic treatment, interferon-alpha/stem cell transplant and implementation of imatinib, respectively, were compared and a lifetime cost-effectiveness model developed. Survival data from population registries, estimated resource use from clinical practice and quality of life estimates were employed. Substantial health gains were noted over time, paralleled by increased treatment costs. Median survival was 1.9, 4.0 and 13 years during the respective time periods. The incremental cost-effectiveness ratio (ICER) between periods III and II was (sic)52 700 per quality-adjusted life year (QALY) gained. An estimated 80% price reduction of imatinib, related to patent expiry, would reduce this ICER to (sic)22 700. Our data from four decades reveal dramatically improved survival in CML, paralleled by ICER levels generally accepted by health authorities.
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| 35. |
- Papareddy, Praveen, et al.
(författare)
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C-terminal Peptides of Tissue Factor Pathway Inhibitor Are Novel Host Defense Molecules
- 2010
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Ingår i: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 285:36, s. 28387-28398
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Tidskriftsartikel (refereegranskat)abstract
- Tissue factor pathway inhibitor (TFPI) inhibits tissue factor-induced coagulation, but may, via its C terminus, also modulate cell surface, heparin, and lipopolysaccharide interactions as well as participate in growth inhibition. Here we show that C-terminal TFPI peptide sequences are antimicrobial against the Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, Gram-positive Bacillus subtilis and Staphylococcus aureus, as well as the fungi Candida albicans and Candida parapsilosis. Fluorescence studies of peptide-treated bacteria, paired with analysis of peptide effects on liposomes, showed that the peptides exerted membrane-breaking effects similar to those seen for the "classic" human antimicrobial peptide LL-37. The killing of E. coli, but not P. aeruginosa, by the C-terminal peptide GGLIKTKRKRKKQRVKIAYEEIFVKNM (GGL27), was enhanced in human plasma and largely abolished in heat-inactivated plasma, a phenomenon linked to generation of antimicrobial C3a and activation of the classic pathway of complement activation. Furthermore, GGL27 displayed anti-endotoxic effects in vitro and in vivo in a mouse model of LPS shock. Importantly, TFPI was found to be expressed in the basal layers of normal epidermis, and was markedly up-regulated in acute skin wounds as well as wound edges of chronic leg ulcers. Furthermore, C-terminal fragments of TFPI were associated with bacteria present in human chronic leg ulcers. These findings suggest a new role for TFPI in cutaneous defense against infections.
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| 36. |
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| 37. |
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| 38. |
- Rydengård, Victoria, et al.
(författare)
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Histidine-rich glycoprotein protects from systemic Candida infection
- 2008
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Ingår i: PLoS pathogens. - : Public Library of Science (PLoS). - 1553-7374 .- 1553-7366. ; 4:8, s. e1000116-
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Tidskriftsartikel (refereegranskat)abstract
- Fungi, such as Candida spp., are commonly found on the skin and at mucosal surfaces. Yet, they rarely cause invasive infections in immunocompetent individuals, an observation reflecting the ability of our innate immune system to control potentially invasive microbes found at biological boundaries. Antimicrobial proteins and peptides are becoming increasingly recognized as important effectors of innate immunity. This is illustrated further by the present investigation, demonstrating a novel antifungal role of histidine-rich glycoprotein (HRG), an abundant and multimodular plasma protein. HRG bound to Candida cells, and induced breaks in the cell walls of the organisms. Correspondingly, HRG preferentially lysed ergosterol-containing liposomes but not cholesterol-containing ones, indicating a specificity for fungal versus other types of eukaryotic membranes. Both antifungal and membrane-rupturing activities of HRG were enhanced at low pH, and mapped to the histidine-rich region of the protein. Ex vivo, HRG-containing plasma as well as fibrin clots exerted antifungal effects. In vivo, Hrg(-/-) mice were susceptible to infection by C. albicans, in contrast to wild-type mice, which were highly resistant to infection. The results demonstrate a key and previously unknown antifungal role of HRG in innate immunity.
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| 39. |
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| 40. |
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| 41. |
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| 42. |
- Wennberg, Anna Lena, 1952-, et al.
(författare)
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Swedish women's food habits during pregnancy up to six months post-partum : a longitudinal study
- 2016
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Ingår i: Sexual & Reproductive HealthCare. - : Elsevier. - 1877-5756 .- 1877-5764. ; 8, s. 31-36
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: Diet influences the health of the foetus and the woman during pregnancy and later in life. It is therefore important to investigate pregnant women's food habits. The aim of this study was to describe women's food habits during pregnancy and up to six months post-partum. Study design: A Food Frequency Questionnaire (VIP-FFQ) was distributed to 163 pregnant women on five occasions during and after pregnancy. Data were analysed using Friedman's ANOVA and a Bonferroni post-hoc test.Main outcome measures: Food habits in relation to the National Food Agency's (NFA) food index.Results: The pregnant women's diets were inadequate according to the NFA food index. A tendency towards an even poorer diet after delivery was identified, something which was related to an increased intake of discretionary food, e.g. sweets, cakes, cookies, crisps, ice cream, and decreased intake of fruit and vegetable. The alcohol consumption was low throughout.Conclusions: The food habits during pregnancy were inadequate compared to recommendations and these habits became unhealthier after delivery. These suggest that dietary counselling needs to be more effective and continued into the lactating period. An increased focus should be given to healthy eating from the life course perspective, not just focus on effects on the foetus and pregnancy outcomes.
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| 43. |
- Wennberg, Anna Lena, et al.
(författare)
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Women's experiences of dietary advice and dietary changes during pregnancy
- 2013
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Ingår i: Midwifery. - : Elsevier BV. - 0266-6138 .- 1532-3099. ; 29:9, s. 1027-1034
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE:to describe women's experiences of dietary information and the change of dietary habits during pregnancy.DESIGN:a qualitative design was used. In 2007 we conducted six focus group interviews using open-ended questions.SETTINGS: five rural and city antenatal clinics in northern Sweden were includedPARTICIPANTS: twenty-three women in mid-pregnancy participated in groups of three to sevenFINDINGS:three domains were found 'Dietary information gain', 'Reactions to dietary information' and 'Dietary management'. The women had to discover dietary information by themselves, and only when health problems or symptoms occurred did they receive guidance from the midwife. Their reactions to the dietary information were 'being confused', 'feeling fear and guilt' and 'being monitored', summed up in 'being uncertain'. The diet was managed by 'checking food content', 'following bodily signals', 'using common sense', and 'making exceptions', summed up as 'being responsible but with a pinch of salt'.KEY CONCLUSIONS:the women expressed problems with dietary changes, but they could mostly manage them on their own. The pregnant women experienced that the midwives gave dietary information and advice first when problems arise. When struggling with diet, the women experienced confusion, and they had to seek information by themselves.IMPLICATIONS FOR PRACTICE:sources of information about diet during pregnancy were experienced as inconsistent and contradictory. Midwives are important in motivation for healthy lifestyle during pregnancy and with sufficient dietary knowledge and counselling skills they can help pregnant women effect dietary changes by providing guidance and support in early pregnancy.
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| 44. |
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