| 1. |
- Madsen, Jan Busk, et al.
(författare)
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Structural and Mechanical Properties of Thin Films of Bovine Submaxillary Mucin (BSM) vs. Porcine Gastric Mucin (PGM) on a Hydrophobic Surface in Aqueous Solutions
- 2016
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Ingår i: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 32:38, s. 9687-9696
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Tidskriftsartikel (refereegranskat)abstract
- The structural and mechanical properties of thin films generated from two types of mucins, namely, bovine submaxillary mucin (BSM) and porcine gastric mucin (PGM) in aqueous environment were investigated with several bulk and surface analytical techniques. Both mucins generated hydrated films on hydrophobic polydimethylsiloxane (PDMS) surfaces from spontaneous adsorption arising from their amphiphilic characteristic. However, BSM formed more elastic films than PGM at neutral pH condition. This structural difference was manifested from the initial film formation processes to the responses to shear stresses applied to the films. Acidification of environmental pH led to strengthening the elastic character of BSM films with increased adsorbed mass, whereas an opposite trend was observed for PGM films. We propose that this contrast originates from that negatively charged motifs are present for both the central and terminal regions of BSM molecule, whereas a similar magnitude of negative charges is localized at the termini of PGM molecule. Given that hydrophobic motifs acting as an anchor are also localized in the terminal region, electrostatic repulsion between anchoring units of PGM molecules on a nonpolar PDMS surface leads to weakening of the mechanical integrity of the films.
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| 2. |
- Sotres, Javier, et al.
(författare)
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Adsorption and nanowear properties of bovine submaxillary mucin films on solid surfaces : influence of solution pH and substrate hydrophobicity
- 2014
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Ingår i: Journal of Colloid and Interface Science. - : Elsevier. - 0021-9797 .- 1095-7103. ; 428, s. 242-250
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Tidskriftsartikel (refereegranskat)abstract
- The adsorption and mechanical stability of bovine submaxillary mucins (BSM) films at solid–liquid interfaces were studied with respect to both substrate hydrophobicity and solution pH. Dynamic light scattering revealed a single peak distribution in neutral aqueous solution (pH 7.4) and a small fraction with enhanced aggregation was observed in acidic solution (pH 3.8). Both substrate hydrophobicity and solution pH were found to affect the spontaneous adsorption of BSM onto solid surfaces; BSM adsorbed more onto hydrophobic surfaces than hydrophilic ones, and adsorbed more at pH 3.8 than at pH 7.4. Thus, the highest “dry” adsorbed mass was observed for hydrophobic surfaces in pH 3.8 solution. However, a highest “wet” adsorbed mass, i.e. which includes the solvent coupled to the film, was observed for hydrophobic surfaces at pH 7.4. The mechanical stability of the films was studied at the nanoscale with an atomic force microscope operated in the friction force spectroscopy mode. Results revealed that BSM films formed on hydrophobic substrates were stronger than those formed on hydrophilic ones. Moreover, the film stability also depended on the ambient pH and stronger films were formed at acidic conditions, i.e. close to the BSM isoelectric point.
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| 3. |
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- 2018
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Ingår i: Nuclear Fusion. - : IOP Publishing. - 1741-4326 .- 0029-5515. ; 58:1
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Forskningsöversikt (refereegranskat)
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