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- Hill, Daniel, et al.
(författare)
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Microfluidic and Transducer Technologies for Lab on a Chip Applications
- 2010
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Ingår i: 2010 ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC). - : IEEE conference proceedings. - 9781424441242 ; , s. 305-307
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Konferensbidrag (refereegranskat)abstract
- Point-of-care diagnostic devices typically require six distinct qualities: they must deliver at least the same sensitivity and selectivity, and for a cost per assay no greater than that of today's central lab technologies, deliver results in a short period of time (<15 min at GP; <2h in hospital), be portable or at least small in scale, and require no or extremely little sample preparation. State-of-the-art devices deliver information of several markers in the same measurement.
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| 2. |
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| 3. |
- Dev Choudhury, Bikash, et al.
(författare)
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Silicon nanopillar arrays with SiO2 overlayer for biosensing application
- 2014
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Ingår i: Optical Materials Express. - 2159-3930. ; 4:7, s. 1345-1354
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Tidskriftsartikel (refereegranskat)abstract
- We present the fabrication of silicon dioxide (SiO2) coated silicon nanopillar array structures and demonstrate their application as sensitive optical biosensors. Colloidal lithography, plasma dry etching and deposition processes are used to fabricate SiO2 coated Si nanopillar arrays with two different diameters and periods. Proof of concept bio recognition experiments are carried out with the bovine serum albumin (BSA)/antiBSA model system using Fourier transform visible and IR spectrometry (FT-VIS-IR) in reflection mode. A limit of detection (LoD) value of 5.2 ng/ml is estimated taking in to account the wavenumber uncertainty in the measurements.
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| 4. |
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| 5. |
- Santiago-Felipe, S., et al.
(författare)
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DVD discs as low-cost diagnostics for GMO screening
- 2014
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Ingår i: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. - : Chemical and Biological Microsystems Society. - 9780979806476 ; , s. 1413-1415
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Konferensbidrag (refereegranskat)abstract
- We demonstrate the incorporation of low-cost and easy-to-fabricate microfluidic chambers on digital versatile discs (DVDs) to perform DNA assays using standard, commercially available and unmodified DVD drives. This simple microarray-based method is applied for the screening of seven prevalent strains of genetically modified organisms (GMOs) in maize and soybean food samples. The detection limit of our system was found to be 7 μg/g, well exceeding the EU regulation limits for GMOs in food products.
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| 8. |
- Tortajada-Genaro, Luis A., et al.
(författare)
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Isothermal solid-phase recombinase polymerase amplification on microfluidic digital versatile discs (DVDs)
- 2015
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Ingår i: RSC Advances. - : Royal Society of Chemistry (RSC). - 2046-2069. ; 5:38, s. 29987-29995
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Tidskriftsartikel (refereegranskat)abstract
- A new advancement in massive DNA-based screening in limited-resource settings is demonstrated through the incorporation of easy-to-fabricate microfluidic chambers on digital versatile discs (DVDs) to perform isothermal recombinase polymerase amplification (RPA) in a microarray format. Standard un-modified DVD discs and commercial drives are used for the low-cost detection method. DNA primers were printed in a microarray format on the polycarbonate surfaces of DVDs with integrated control spots to guarantee the absence of false-negatives and false-positives. The solid-phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of solutions through the inlet and by controlling the flow-movement by DVD drive centrifugation. The final disc with reaction products was inserted into a DVD player and microarray images were captured and automatically processed. This simple approach was applied for the screening of genetically modified organisms (GMOs) in food samples. The limit of detection was 7 mu g g(-1), which is well below the EU regulation limit for GMOs in food products. Therefore, the only required materials for food safety monitoring were standard store-bought DVDs, plastic chambers, tips, pipettes, an oven, and a standard DVD drive. The proposed strategy allows an integrated microarray system with low manipulation, reduced sample volume, and portability, which are beneficial for low-resource settings.
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