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Sökning: WFRF:(Marrazza G)

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1.
  • Al-Khafaji, Q A M, et al. (författare)
  • An Electrochemical Immunoassay for HER2 Detection
  • 2012
  • Ingår i: Electroanalysis. - : Wiley-VCH Verlag Berlin. - 1040-0397 .- 1521-4109. ; 24:4, s. 735-742
  • Tidskriftsartikel (refereegranskat)abstract
    • In this paper, a simple and sensitive approach for human epidermal growth factor receptor 2 (HER2) detection is presented, using antibody-functionalised magnetic beads coupled to screen-printed cells. The immunoassay is based on a sandwich format in which a primary monoclonal antibody anti-HER2 is coupled to protein A modified magnetic beads. The modified beads are then used to capture the protein from the sample solution and a sandwich assay is performed by adding a secondary monoclonal antibody anti-HER2 labelled with biotin. The enzyme alkaline phosphatase (AP) conjugated with streptavidin and its substrate (1-naphthyl-phosphate) are then used for the electrochemical detection by differential pulse voltammetry (DPV). The experimental conditions for the immunoassay were optimised. The performance of the assay in terms of sensitivity, reproducibility and selectivity has been studied in buffer and serum samples from hospital patients.
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2.
  • Lucarelli, F, et al. (författare)
  • Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors
  • 2004
  • Ingår i: Biosensors & bioelectronics. - : Elsevier Science B.V., Amsterdam.. - 0956-5663 .- 1873-4235. ; 19:6, s. 515-530
  • Forskningsöversikt (refereegranskat)abstract
    • Genosensor technology relying on the use of carbon and gold electrodes is reviewed. The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discussed in detail with separate sections devoted to label-free and newly emerging magnetic assays. Special emphasis has been given to protocols that have been used with real DNA samples. (C) 2003 Elsevier B.V. All fights reserved.
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3.
  • NEWMAN, JD, et al. (författare)
  • INK-JET PRINTING FOR THE FABRICATION OF AMPEROMETRIC GLUCOSE BIOSENSORS
  • 1992
  • Ingår i: Analytica Chimica Acta. - : Elsevier Science B.V., Amsterdam.. - 0003-2670 .- 1873-4324. ; 262:1, s. 13-17
  • Tidskriftsartikel (refereegranskat)abstract
    • Ink-jet printing has been demonstrated as a manufacturing technique that facilitates the rapid, reproducible and economical production of amperometric glucose biosensors. Glucose was chosen as the analyte for demonstrating the process. For eight electrodes produced, the relative standard deviation of the response was less than 5%. The technique is extremely versatile, and will enable a wide variety of reagents to be placed on virtually any sensor design. This technique will be of particular benefit for the mass manufacture of intricate devices, where existing production techniques, such as screen-printing, may not be suitable.
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4.
  • Rasinger, JD, et al. (författare)
  • Evaluation of an FIA operated amperometric bacterial biosensor, based on pseudomonas putida F1 for the detection of benzene, toluene, ethylbenzene, and xylenes (BTEX)
  • 2005
  • Ingår i: Analytical Letters. - : Taylor andamp;amp; Francis. - 0003-2719 .- 1532-236X. ; 38:10, s. 1531-1547
  • Tidskriftsartikel (refereegranskat)abstract
    • Recently, the development and optimization of a flow injection analysis (FIA) operated bacterial biosensor based on the aerobic catabolism of Pseudomonas putida ML2 was reported in the literature (Lanyon et al. 2004, 2005). By adapting information from these reports, we investigated whether operating parameters and procedures of the benzene biosensor could be directly applied to a new system based on a different bacterial strain for the detection of the whole benzene, toluene, ethylbenzene, and xylenes range. Cells of the investigated bacterial strain, Pseudomonas putida F1, were immobilized between two cellulose acetate membranes and fixed onto a Clark dissolved oxygen electrode. The P. putida F1 aerobically degrades benzene, toluene, and ethylbenzene (BTE) (Cho et al. 2000). The BTE biosensor in kinetic mode FIA displayed a linear range of 0.02-0.14 mM benzene (response time: 5 min, baseline recovery time: 15 min), 0.05-0.2 mM toluene (response time: 8 min, baseline recovery time: 20 min), and 0.1-0.2 mM ethylbenzene (response time: 12 min, baseline recovery time: 30 min), respectively. Due to the differences in sensitivity, response, and baseline recovery times for BTE, it was possible to differentiate each compound in mixtures of these volatile organic compounds (VOCs). No response for xylenes could be obtained since they cannot be completely metabolized by this bacterial strain. However, it was reported that the range of compounds degradable by P. putida F1 can possibly be expanded by cultivating the cells on different carbon sources (Choi et al. 2003). The sensor showed good intra- and interassay reproducibility, and all obtained results were comparable with those reported in the literature. The demonstrated reproducibility and the simplicity and ease of use as well as the portability for in situ measurements indicates that the biosensor could be suitable as a reliable initial warning device for elevated BTE levels in indoor and outdoor environments.
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