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| 2. |
- Malmsten, M, et al.
(författare)
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Adsorption of apolipoprotein B at phospholipid model surfaces
- 1995
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Ingår i: Journal of Colloid and Interface Science. - 0021-9797 .- 1095-7103. ; 172, s. 485-493
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Tidskriftsartikel (refereegranskat)abstract
- The adsorption of apolipoprotein B (Apo B) at a series of surfaces was investigated with in situ ellipsometry. For silica and methylated silica, the adsorbed amount (G), the adsorbed layer thickness (del) and the mean adsorbed layer refractive index (nf) were obtained by a procedure involving studies of the bare substrate at two different ambient refractive indices, as well as four-zone averaging. The adsorbed amount of Apo B is much higher at silica than at methylated silica. Despite this, the adsorbed layer thickness is the same at the two surfaces, and the adsorbed layer formation proceeds similarly. In both cases, the adsorbed layer formation occurs through the adsorption of Apo B molecules in an essentially random orientation, the difference between silica and methylated silica being the number of molecules adsorbed per unit area. Furthermore, the adsorption of Apo B at phospholipid surfaces was investigated. It was found that the adsorption at phosphatidylcholine (PC) was quite limited, whereas that at phosphatidic acid (PA) was substantial. Studies with mixed PA/PC layers showed that the Apo B adsorption depends on the mixed phospholipid layer composition in an essentially linear fashion. Finally, mixed phospholipid layers of PC and ganglioside GM1, as well as phosphatidylinositol (PI) layers, showed a dramatic preferential adsorption of Apo B over, e. g. human serum albumin (HSA), IgG, fibronectin and fibrinogen.
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| 3. |
- Masquelier, M, et al.
(författare)
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Plasma stability and cytotoxicity of lipophilic daunorubicin derivatives incorporated into low density lipoproteins
- 2000
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Ingår i: European Journal of Medicinal Chemistry. - 0223-5234 .- 1768-3254. ; 35:4, s. 429-438
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Tidskriftsartikel (refereegranskat)abstract
- The selective targeting of antineoplastic drugs to tumours by incorporation in low density lipoproteins (LDL) is an attractive possibility if the drug-LDL complex remains stable in the circulation and is taken up by the tumour. In previous studies we have shown that vincristine- and N- trifluoroacetyladriamycin-14-valerate-LDL complexes were unstable in vivo. We synthesized five N-substituted lipophilic derivatives of daunorubicin and studied their incorporation into LDL. Three out of five daunorubicin derivatives incorporated successfully into LDL. In vitro these complexes were more cytotoxic towards LDL receptor positive Chinese hamster ovary cells than LDL receptor negative cells. Non-specific cytotoxicity was explained by slow dissociation of the drug-LDL complex in plasma. Our results underline the importance of careful studies of plasma stability when investigating lipoproteins and other carriers in drug targeting.
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| 5. |
- Masquelier, M, et al.
(författare)
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Low density lipoprotein as a carrier of cytostatics in cancer chemotherapy : Study of stability of drug-carrier complexes in blood
- 2000
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Ingår i: Journal of drug targeting (Print). - 1061-186X .- 1029-2330. ; 8:3, s. 155-164
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Tidskriftsartikel (refereegranskat)abstract
- Several solid tumour and leukemia cell types have a higher low density lipoprotein (LDL) uptake than the corresponding normal cells. We are investigating the possibilities to use LDL as a drug carrier to increase the selectivity of antineoplastic drugs in cancer chemotherapy. We have developed a method to incorporate lipophilic cytotoxic agents without interfering with the in vitro and in vivo properties of LDL, In this study, we examined the stability of some drug-LDL complexes in blood and plasma as this is an important prerequisite to achieve a selective therapy. The in vitro dialysis of N-trifluoroacetyl-adriamycin-14-valerat-LDL (AD-32-LDL) against plasma revealed a slow dissociation of the complex. The same method showed a fast and total leakage of paclitaxel from paclitaxel-LDL into the plasma chamber. The dissociation of paclitaxel was confirmed by an autoradiographic study of the distribution of paclitaxel-LDL in tumour-bearing mice. In patients with leukemia the rapid plasma dissociation of AD-32 from LDL illustrated a much higher in vivo instability of this complex. With this method, cholesteryl-linoleate only could be incorporated into LDL in a stable manner as shown by dialysis and autoradiography results. The incorporation of cytotoxic drug derivatives, containing lipophilic anchors, is now under study in order to obtain LDL complexes with better plasma stability.
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| 7. |
- Pohanka, A, et al.
(författare)
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Quantification of valproic acid in dried blood spots
- 2014
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Ingår i: Scandinavian journal of clinical and laboratory investigation. - : Informa UK Limited. - 1502-7686 .- 0036-5513. ; 74:7, s. 648-652
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Tidskriftsartikel (refereegranskat)
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| 8. |
- Ponrouch, A., et al.
(författare)
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Towards high energy density sodium ion batteries through electrolyte optimization
- 2013
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Ingår i: Energy and Environmental Sciences. - : Royal Society of Chemistry (RSC). - 1754-5692 .- 1754-5706. ; 6:8, s. 2361-2369
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Tidskriftsartikel (refereegranskat)abstract
- A comprehensive study is reported entailing optimization of sodium ion electrolyte formulation and compatibility studies with positive and negative electrode materials. EC:PC:DMC and EC:PC:DME were found to exhibit optimum ionic conductivities and lower viscosities. Yet, hard carbon negative electrode materials tested in such electrolytes exhibit significant differences in performance, rooted in the different resistivity of the SEI, which results in too large polarization and concomitant loss of capacity at low potentials when DME is used as a co-solvent. EC0.45:PC0.45:DMC0.1 was found to be the optimum composition resulting in good rate capability and high capacity upon sustained cycling for hard carbon electrodes. Its compatibility with positive Na3V2(PO4)(2)F-3 (NVPF) electrodes was also confirmed, which led to the assembly of full Na-ion cells displaying an operation voltage of 3.65 V, very low polarisation and excellent capacity retention upon cycling with ca. 97 mA h g(-1) of NVPF after more than 120 cycles together with satisfactory coulombic efficiency (>98.5%) and very good power performance. Such values lead to energy densities comparable to those of the current state-of-the-art lithium-ion technology.
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| 9. |
- Razmara, M, et al.
(författare)
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Glycoprotein IIb/IIIa blockade inhibits platelet aminophospholipid exposure by potentiating translocase and attenuating scramblase activity.
- 2007
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Ingår i: Cellular and Molecular Life Sciences (CMLS). - : Springer Science and Business Media LLC. - 1420-682X .- 1420-9071. ; 64:7-8, s. 999-1008
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Tidskriftsartikel (refereegranskat)abstract
- The present study investigated the mechanisms underlying the inhibition of platelet phosphatidylserine (PS) exposure by GPIIb/IIIa blockade. Platelet PS exposure induced by thrombin stimulation was cell-cell contact dependent. GPIIb/IIIa blockade by c7E3 or SR121566 inhibited thrombin-induced platelet PS exposure. Thrombin stimulation induced mild, while A23187 induced extensive platelet-derived microparticle (PDMP) generation. Thrombin-induced PDMP generation was not inhibited by GPIIb/IIIa blockade. Aminophospholipid translocase activity was reduced upon platelet activation by thrombin. The reduction of non-PS-exposing platelets was attenuated by GPIIb/IIIa blockade, while little translocase activity was seen in PS-exposing platelets. Thrombin increased scramblase activity slightly in non-PS-exposing platelets, which was inhibited by GPIIb/IIIa blockade, and markedly enhanced scramblase activity in PS-exposing platelets. Activation of platelet calpain and caspase-3 or cytosolic calcium mobilization were not altered by GPIIb/IIIa inhibition. Thus, GPIIb/IIIa blockade inhibits platelet PS exposure by enhancing translocase activity and attenuating scramblase activity, but does not inhibit PDMP generation.
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