| 1. |
- Lewin, Harris A., et al.
(författare)
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The Earth BioGenome Project 2020 : Starting the clock
- 2022
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences (PNAS). - 0027-8424 .- 1091-6490. ; 119:4
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 2. |
- Creek, D. J., et al.
(författare)
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Probing the Metabolic Network in Bloodstream-Form Trypanosoma brucei Using Untargeted Metabolomics with Stable Isotope Labelled Glucose
- 2015
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Ingår i: PLoS Pathogens. - : Public Library of Science (PLoS). - 1553-7366 .- 1553-7374. ; 11:3, s. 1-25
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Tidskriftsartikel (refereegranskat)abstract
- Metabolomics coupled with heavy-atom isotope-labelled glucose has been used to probe the metabolic pathways active in cultured bloodstream form trypomastigotes of Trypanosoma brucei, a parasite responsible for human African trypanosomiasis. Glucose enters many branches of metabolism beyond glycolysis, which has been widely held to be the sole route of glucose metabolism. Whilst pyruvate is the major end-product of glucose catabolism, its transamination product, alanine, is also produced in significant quantities. The oxidative branch of the pentose phosphate pathway is operative, although the non-oxidative branch is not. Ribose 5-phosphate generated through this pathway distributes widely into nucleotide synthesis and other branches of metabolism. Acetate, derived from glucose, is found associated with a range of acetylated amino acids and, to a lesser extent, fatty acids; while labelled glycerol is found in many glycerophospholipids. Glucose also enters inositol and several sugar nucleotides that serve as precursors to macromolecule biosynthesis. Although a Krebs cycle is not operative, malate, fumarate and succinate, primarily labelled in three carbons, were present, indicating an origin from phosphoenolpyruvate via oxaloacetate. Interestingly, the enzyme responsible for conversion of phosphoenolpyruvate to oxaloacetate, phosphoenolpyruvate carboxykinase, was shown to be essential to the bloodstream form trypanosomes, as demonstrated by the lethal phenotype induced by RNAi-mediated downregulation of its expression. In addition, glucose derivatives enter pyrimidine biosynthesis via oxaloacetate as a precursor to aspartate and orotate.
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| 3. |
- Davies, R., et al.
(författare)
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BIM in Europe : Innovation networks in the construction sectors of Sweden, France and the UK
- 2015
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Ingår i: Proceedings of the 31st Annual Association of Researchers in Construction Management Conference, ARCOM 2015. - : Association of Researchers in Construction Management. - 9780955239090 ; , s. 1135-1144
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Konferensbidrag (refereegranskat)abstract
- European countries are developing or implementing policies that promote or require the use of Building Information Modelling (BIM) and give BIM a central role in strategies for national sector-level transformation. It is necessary to understand BIM as a systemic innovation that is enacted and adopted by firms, projects and users but also by national actors. The Industrial Marketing and Purchasing (IMP) approach has shown how the evolution of innovations can be understood in terms of networks in which actors mobilise and combine technical and social resources in order to perform activities-the Actors-Activities-Resources (ARA) model. A comparative study of BIM adoption in France, Sweden and the UK was undertaken using data from independent country-specific research projects and a pooled desktop study. A grid was developed based on the ARA model that provided a framework to inform data collection and analysis salient for explaining the extent, processes and type of adoption of BIM in each country. Similarities between countries included: the importance of large and international firms in the innovation network; and project types (non-residential public buildings and either complex or repetitive building types). Differences were found in, for example, the activities and national institutions of architecture and the policy positions and mechanisms of government actors. The analysis highlights both the value and some limitations of a country-level focus and provides a basis for thoroughgoing network analysis.
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